Document rpR4nD1Nyb42XmeenmmJKabBE

Younger Laboratories BiadtemUti . .. PiiMmx&doqUii-... Attaltfiii 6620 TKLEghaph road St, Loula 29* MISSOURI PlIONBl TILDEN 6*2640 GeAiijficate c^- /Inabf&ii SUBJECT - Toxicological Investigation Ofi Monsanto Sample Number 36 Monsanto Project Number 1-62-31* Aroclor 12l2 June 19th, 1962 STUDY CONDUCTED FOR Monsanto Chemical Company, St, Louis, Missouri EXPERIMENTAL PROCEDURE - t.) Oral Ll-80 (Rots) The undiluted ccmoound ua oibe to Sprague-Iawley strain albino rats. After the appri female rets we, intervals at ing data for Miniakim Lethal iope wae determined, groups of male and 'fed in increasing dps/s at increments of 0.1 fractional log r levels deb$te*<iW^/61anket the toxicity range thereby supply- culntion of tns--which was lone according to a modification of tho method E. J. d</^eer. Observations we suocumbod were toxic symptoms and the viscera of the animals that microscopically. Hie data are shewn in Table I. B) Skin Absorption ffLI) (Rabbits) Tho undiluted compound was applied in increasing doses at increments of 0.2 fractional log intervals to the closely clipped, intact skin of New Zealand white female rabbits. Tho treated areas were covered with plastic strips and the animals placed in wooden stock3 for periods up to twenty-four hours, after which time they were assigned to individual cages. Observations wore made for toxic symptoms and the viscera of the animals that succumbed were examined macroscopically. The data are shown in Table H. MOMS 037571 ThW confidralUl ..port 1, not to bo und in iny form o[ .dv.rti.in. without wiittun permiMion Toi Monsanto Chemical mpany St, Louis, Missouri Younger Laboratories Certificate of Analysis - Pago 2 (6/19/62) - 1-62-34 SUMMARY Aroclor 1242 A) Oral Lr'80 (Rate) The Oral LP80 for male and female rats was placed at 8650 milligrams per kilogram with lower and upper limits of 7610 to 977? milligrams per kilogran. The compound was classed as practically non-toxic by oral ingestion in male and female rats. B) Skin Absorption MLG (Rabbits) The Minimum Le$ial l ose by Skin Absorption in female rabbits, was found to be greater thiuT?9l Milligrams per kilogram and less than 1260 milligrams per kilogram, \Aniaal #2 was moderately affected but was not in danger of collapse. The compound was classed as moderately toxic by skin absorption in female rabbits. YOUNGER LABORATORIES MONS 03 75 22 Toi Monsto Chemioa. ompany St, Louie, Missouri lounger Laberatoriee Oertifioate of Analysis - Page 3 (6/19/62) - T-62-JJ* TABLE I THE ORAL LD eo CP ' Aroclor 12lt2' FOR RATS Scrapie Fed Undiluted Animal No. - Sam Weight On* 1- Female 2- Female 3- Female Male 5- Male 6- Male 7- Female 8- Female 9- Female 10- Male 11- Male 12- Kale 13- Female IV Female 15- Female 16- Kale 17- Male 18- Male 19- Ftaale 20- Female 21- Male 220 220 225 2l*0 225 235 210 215 225 225 2lt5 230 215 205 220 235 250 21t0 215 215 230 Does Mg. /Kg. 6,310 6,310 6,310 6,310 6,310 7,9U0 7.9U0 7,9kO 7,9U0 7,9l0 7,9UO 10,000 10,000 10,000 10,000 10,000 12,600 12,600 12,600 12,600 12,600 Fate Survived Survived Survived Survived Survived Survived Died Died Survived Died Survived Died Died Died Died Survived Died Died Died Died Died niSCUSSICM The Oral Lnso for mala and fanale rata was plsoed at 8650 mllllgrama per kilogram with lower and upper limit* of 7610 to 9775 milligrams per kilogram. The compound was classed as practically non-toxic by oral ingestion in male and female rats. Survlvd time was about twenty-four hours to three days with most deaths occurring in two days. Tooclo symptoms included diarrhea, loss of appetite, increasing weakness, dyspnea, and oollapee. t autopsy there was liver and renal hyperemia maeroaoopleelly. MGNS 037573 TO. Konaanto Chaaical .^kpany Si, Lewie, Miaeourl Teuagor Labaretoriae Oertifieate at Ar>aly-eie - Pag* 3 (6/l?/62) - T-62-JI* 1ABLI II THE KDUJKM LETHAL DOSE OF 'Aroolor 121*2' BT SEIM ABSORPTICir W RABBITS Sample Applied Undiluted Anlaal No, - Sax 1 - Faaala 2 - Faaala 3 - Faaala It - Faaala S - Faaala 6 - Faaala 7 - Faaala Weight Kg. 2.It 2.0 2.2 2.3 2.1 2.2 2.1t Doaa *C0L 7?lt 1260 2000 2000 3160 $00.0 Weight Change $ Day* Later U. - 0.2 - 0.2 - 0.U - 0.3 - 0.1* -- - o.$ Fata Surrirad Surriwed Mad - 7 Daya Died - 10 Daya Died - 6 Daya Died - It Daya Diad - 6 Daya DXSCU8SICM - The Minima Lethal Dos* by Bkla Abaorptlon la faaala rabbit* out found to b* greater than ?9l| nllligraae par kilogre and la a* than 1260 allllgraa* par kilogram. Anlaal ft U moderately affootod hut vaa net la dangor of oollapa*. lb* eoapound vaa slaaaed aa moderately teode bp skin abeorptiaa In faaale rebbite. Surriral tlaa vaa four to ton day*. Teode aymptaae included poor appetite with aarkad weight loe, ocoalienal aqueallng after aanral days, and oollapae. At autopay tfcaao waa llrar dlaoolaratioa (light color and aoft), and pulaonary congaatloa naoro*opl**Uy. MONS 037574