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z c Perfluorochemicals in Mississippi River Pool 2: 2012 Update Fish, benthic invertebrates, water, and sediment were tested for PFCs in 2012. Results are summarized and compared to previous results from Pool 2. May 2013 Authors Bruce Monson Contributors / acknowledgements Paul Hoff Phil Monson Joel Stiras Harold Wiegner Editing and Graphic Design Cover photo: Upper Midwest Environmental Sciences Center. http://www.umesc.usgs.gov/aerial_photos/s/s31g 1_1997ob.html The MPCA is reducing printing and mailing costs by using the Internet to distribute reports and information to wider audience. Visit our website for more information. MPCA reports are printed on 100 percent postconsumer recycled content paper manufactured without chlorine or chlorine derivatives. Minnesota Pollution Control Agency 520 Lafayette Road North | Saint Paul, MN 55155-4194 | www.pca.state.mn.us | 651-296-6300 Toll free 800-657-3864 | TTY 651-282-5332 This report is available in alternative formats upon request, and online at www.pca.state.mn.us Document number: c-pfc1-21 Table of Contents Abbreviations glossary ........................................................................................................... i Executive summary ............................................................................................................ E-1 Introduction .......................................................................................................................... 1 What are PFCs and why are they an environmental contaminant?......................................................... 1 Mississippi River Pool 2 ........................................................................................................................ 1 Why this study? ................................................................................................................................... 2 Methods ............................................................................................................................... 3 Fish collection ...................................................................................................................................... 3 Water collection .................................................................................................................................. 3 Sediment and benthic macroinvertebrate collection .............................................................................. 4 Laboratory analysis.............................................................................................................................. 4 Quality assurance ................................................................................................................................ 4 Results and discussion ........................................................................................................... 5 Surface water ...................................................................................................................................... 5 Fish ..................................................................................................................................................... 5 Bioaccumulation factors and site-specific water quality criterion ........................................................... 6 Sediments............................................................................................................................................ 7 Benthic invertebrates ........................................................................................................................... 8 Conclusions.......................................................................................................................................... 9 References ........................................................................................................................................... 9 Tables ................................................................................................................................. 11 Figures ................................................................................................................................ 14 Appendix A: Fish and Water Data........................................................................................ 23 Appendix B: Benthic Macroinvertebrate and Sediment Data ............................................... 55 Tables Table 1. PFOS concentrations (ng/L) in water samples collected at the same twelve stations in 2009 and 2012; A, B, and C refer to consecutive grab samples collected at each station; in 2012 two samples from Stations 1 and 5 were used for laboratory matrix spikes. ................................................... 11 Table 2. Descriptive statistics for PFOS (ng/g -ww) in the five target fish species; combined results for 2004-2012 ................................................................................................................................................... 11 Table 3. PFOS concentrations (ng/g -ww) for each fish species and year from all of Pool 2 (left) and only Lower Pool 2--Section 4 (right); the top row of each species is for all years; results from the study in 2011 used lower Reach 9 and Reach 10 to represent the Lower Pool 2; in 2004-2008 all fish were collected in the Lower Pool 2............................................................................................................. 12 Table 4. Summary statistics for sediment and invertebrate measurements ............................................. 13 Table 5. Replicates of sediment PFOS concentrations with matching invertebrate PFOS concentrations and BSAF ............................................................................................................................ 13 Figures Figure ES-1. Locations of Sections and Stations and summary tables of mean PFOS concentrations in fish and water ...................................................................................................................................... ES-3 Figure ES-2. PFOS concentrations in sediment and benthic invertebrates in Pool 2 from 2011-2012 ... ES-4 Figure 1. Pool 2 showing river miles between Lock & Dam No. 1 at river mile 847.6 to Lock & Dam No. 2 at river mile 815.6 (~32.5 miles); shows four sections and PFC water stations for 2009 and 2012........... 14 Figure 2. Water levels at Mississippi River South St. Paul: (a) record between 1 July 2009 and 1 October 2012; (b) hourly water levels on sampling days (http://www.mvpwc.usace.army.mil/dcp/SSPM5.html)......................................................................................................... 15 Figure 3. Fish-collection runs in Pool 2: a. entire Pool 2 showing reaches and runs; b. runs in Section 4.................................................................................................................................................. 16 Figure 4. Box-whisker plots of PFOS concentrations in Lower Pool 2 by year for (a) smallmouth bass, (b) carp, and (c) freshwater drum............................................................................................................... 17 Figure 5. Species-Section matrix showing distributions of PFOS in 2009 (red) and 2012 (blue); horizontal mid-line is the median; vertical lines are PFOS thresholds of 40 ng/g and 200 ng/g................ 18 Figure 6. PFOS concentrations by fish-collection runs in Section 4 (2012) ................................................ 19 Figure 7. PFOS concentrations (dry weight) in surface sediments collected between November 2011 and November 2012; concentrations are color coded and insert map shows measured concentrations in Section 4 of Pool 2 ...................................................................................................................................... 20 Figure 8. PFOS concentrations in sediment (ng/g dry weight) and benthic macroinvertebrates (ng/g wet weight)........................................................................................................................................ 21 Figure 9. Relationship between invertebrate and sediment PFOS concentrations (both wet weight and log-scale) .............................................................................................................................................. 22 Abbreviations glossary Groups PFCs - perfluorochemicals or perfluorinated compounds PASs - perfluoroalkyl surfactants PFCAs - perfluorocarboxylic acid PFSAs - polyfluorinated alkyl substances FTOH - fluorotelomer alcohols PFAAs - perfluoroalkyl acids Individual PFBA - perfluorobutanoic acid (C4 Acid) PFBS - perfluorobutane sulfonate (C4 Sulfonate) PFPeA - perfluoropentanoic acid (C5 Acid) PFHxA - perfluorohexanoic acid (C6 Acid) PFHxS - perfluorohexane sulfonate (C6 Sulfonate) PFHpA - perfluoroheptanoic acid (C7 Acid) PFOA - perfluorooctanoic acid (C8 Acid) PFOS - perfluorooctane sulfonate (C8 Sulfonate) PFOSA - perfluorooctane sulfonamide (C8 Sulfonamide, branched) PFNA - perfluorononanoic acid (C9 Acid) PFDA - perfluorodecanoic acid (C10 Acid) PFUnA - perfluoroundecanoic acid (C11 Acid) PFDoA - perfluorododecanoic acid (C12 Acid) Other abbreviations AFFF - aqueous fire-fighting foam ECF - electrochemical fluorination FCA - fish consumption advisory MDH - Minnesota Department of Health MDNR - Minnesota Department of Natural Resources MPCA - Minnesota Pollution Control Agency WWTP - wastewater treatment plant Perfluorochemicals in Mississippi River Pool 2 May 2013 i Minnesota Pollution Control Agency Executive summary Mississippi River Pool 2 is the 32.5 mile reach between Lock & Dam No. 1 (Ford Dam) and Lock & Dam No. 2 (Hastings Dam), dissecting Minneapolis, St. Paul, and communities south of St. Paul to Hastings. Perfluorochemicals (PFCs) were produced by 3M between the 1950s and 2002 at its manufacturing plant on the river in Cottage Grove, Minnesota. The Minnesota Pollution Control Agency (MPCA) began testing fish from Pool 2 for PFCs in 2004. Results showed high levels of one PFC, perfluorooctane sulfonate (PFOS), in the fish. Since 2004, fish from many lakes and rivers in Minnesota have been tested for PFCs. Several Pool 2 lakes in Twin Cities Metropolitan Area had high PFOS concentrations in the fish that led to fish consumption advisories from the Minnesota Department of Health (MDH) and impairment classifications by the MPCA. The MDH developed a reference dose for PFOS that is used as the basis for fish consumption advice. Mean PFOS concentrations in fish greater than 40 parts per billion (ppb or nanogram per gram, ng/g) to 200 ppb are assigned meal advice of one meal per week; greater than 200 to 800 ppb, the advice is one meal per month, and above 800 ppb, do not eat. In 2009, freshwater drum from Pool 2 had the highest PFOS concentrations among the five species tested and drum were assigned a consumption advisory of one meal per month, based on a mean PFOS concentration of 229 ppb. Smallmouth bass exceeded the threshold for a meal per month with a mean PFOS concentration of 209 ppb when data from 2004 to 2009 are combined; however, meal advice was not given for this species in Pool 2 because only catchand-release fishing is allowed (http://www.health.state.mn.us/divs/eh/fish/). Pool 2 had been listed as impaired for PFOS in fish tissue in 2008 because of a meal per month advisory for bluegill sunfish. It stayed on the impaired waters list in 2010 because of the freshwater drum. This report presents the results of an intensive monitoring of PFCs in fish, benthic macroinvertebrates (sediment-dwelling insects), water, and sediments in 2012, which was a follow-up to the first intensive study in 2009 (MPCA 2010) and a second study conducted by 3M in 2011 (Cardno-Entrix 2011). The sampling protocols for fish and water in 2012 were identical to the 2009 sampling protocols, with the exception that in 2012 the fish-collection runs were georeferenced. Split sampling of water and fish, which occurred in 2009 with 3M, was not repeated in 2012. The sampling protocol by 3M's contractor, Cardno-Entrix, for the 2011 study differed from the 2009 MPCA study in site locations for water samples, number of fish, and species collected. All three studies collected bluegill sunfish, freshwater drum, smallmouth bass, and white bass. Common carp were collected in 2009 and 2012. Drum had not been sampled prior to 2009, because it was not considered a typical sport fish. Based on the Minnesota Department of Natural Resources' (MDNR) observation that drum have been harvested in Pool 2 for human consumption, it was included as a target species in 2009 and subsequent years. To characterize possible differences within Pool 2, it was divided into four sections for the monitoring in 2009 and 2012 (Figure ES-1). Three water stations in each section were sampled in triplicate in 2009 and 2012, for a total of 12 stations. The 3M study in 2011 divided Pool 2 into ten reaches; three water stations in 10 reaches gave a total of 30 stations in 2011. Perfluorochemicals in Mississippi River Pool 2 May 2013 ES-1 Minnesota Pollution Control Agency Sediment and benthic macroinvertebrates had not been collected in 2009 or 2011. Testing of sediment and invertebrates were added in 2012 to better understand the extent of PFC contamination in the Pool 2 food web, and to further characterize any differences among sampling stations. Fish can potentially swim throughout Pool 2, whereas sediment and the insect nymphs in the sediments are stationary. The results of fish and water PFOS concentrations are summarized for 2009 and 2012 in Figure ES-1. PFOS was detected in all but two of the 296 fish collected in 2012 from Pool 2. Pool-wide means decreased substantially between 2009 and 2012 for four of the five fish species. Only carp showed an increase in the mean PFOS concentration. The means for each of the four sections in the river indicate the higher pool-wide PFOS concentration for carp in 2012 was caused by an increase in Section 4 despite drops in the mean in the other sections. The highest PFOS concentrations in fish coincided with the location of the highest water concentrations in Section 4. Of the five targeted fish species in Pool 2, freshwater drum had the highest average PFOS concentration in 2009, while carp had the highest concentration in 2012. In the water samples from 2012, perfluorobutanoic acid (PFBA) was detected at all 12 water-collection stations. Perfluorooctanoic acid (PFOA) and perfluorohexanoic acid (PFHxA) were detected in Sections 2, 3, and 4. The highest water concentrations of PFCs were found in Section 4 (lower Pool 2). Three other PFCs were detected in Section 4: perfluoropentanoic acid (PFPeA), perfluorobutane sulfonate (PFBS), and perfluorohexane sulfonate (PFHxS). In 2012, PFOS concentrations in water continued to show a similar geographic pattern to 2009 results. PFOS concentrations in water were generally below the detection limit in the upper 10 sampling stations. Stations 6, 7, and 8, had detectable PFOS concentrations in 2009, but were below detection in 2012 (except for one of the samples at Station 8). PFOS concentrations in water were relatively high at the lower two stations, 11 and 12. Station 11 was immediately downstream of the 3M Cottage Grove Center and Station 12 was farther downstream. PFOS concentrations at the two downstream stations were higher in 2012 than in 2009. In both years, the PFOS concentrations at these two downstream stations were well above the site-specific water quality criteria, 7 ng/L, established in 2009. The reason for the higher concentrations in 2012 is not known, but a comparison of PFOS concentrations and river water levels for 2009, 2011, and 2012 indicated the higher level in 2012 was not because of lower flows and less dilution from point source discharges. Site-specific water quality criteria to protect fish tissue for human consumption are calculated when a water concentration is needed for regulatory purposes. To calculate the water quality criterion, the needed site-specific information is the ratio of fish tissue concentration to water concentration, referred to as the bioaccumulation factor (BAF). BAFs were calculated for PFOS using the water and fish data in 2009 and updated in 2012. Species-specific mean BAFs for PFOS based on the 2012 data ranged from 2700 L/kg to 4600 L/kg; compared to the range of 4000 to 6000 L/Kg from the 2009 data. Lower average BAFs in 2012 compared to 2009 resulted in the site-specific water quality criterion for PFOS in Pool 2 increasing from 7 ng/L to 14 ng/L. Sediment and benthic invertebrate PFOS concentrations were detectable but relatively low for sites upstream of the 3M Cottage Grove Center (Figure ES-2). The high PFOS concentrations in sediment and invertebrates near Station 11 reinforce the results from fish and water. In summary, PFOS concentrations in fish have declined in Pool 2; however, PFOS concentrations remain high in fish, invertebrates, sediments, and water from the lower section of Pool 2. Perfluorochemicals in Mississippi River Pool 2 May 2013 ES-2 Minnesota Pollution Control Agency Figure ES-1. Locations of Sections and Stations and summary tables of mean PFOS concentrations in fish and water Perfluorochemicals in Mississippi River Pool 2 May 2013 ES-3 Minnesota Pollution Control Agency Figure ES-2. PFOS concentrations in sediment and benthic invertebrates in Pool 2 from 2011-2012 Perfluorochemicals in Mississippi River Pool 2 May 2013 ES-4 Minnesota Pollution Control Agency Introduction What are PFCs and why are they an environmental contaminant? Perfluorochemicals (PFCs)--also known as perfluorinated compounds--are composed of a carbon chain saturated with fluorine atoms, with a functional group at the end of the chain. The functional groups are typically a sulfonate (-SO3) or a carboxylic acid group (-COOH). The functional group makes the PFC soluble in water (hydrophilic), whereas the carbon chain makes the PFC molecule soluble in lipids (lipophilic) and resistant to water (hydrophobic). The unique properties of PFCs have been exploited for numerous consumer products, most notably fire-fighting foam, stain protection, and non-stick surfaces. PFCs emerged as a global pollutant in 2001 when scientists reported perfluorooctane sulfonate (PFOS) in wildlife throughout the world (Giesy and Kannan, 2001). Subsequently, PFCs were described as "globally distributed, environmentally persistent, bioaccumulative, and potentially harmful" (Giesy and Kannan, 2002). PFOS was shown to be the predominant PFC in most fish (Houde et al., 2006). Detection limits for PFCs generally range from 2.5 to 5 nanograms per gram (ng/g), or parts per billion (ppb). Although there are naturally occurring fluorinated organic compounds that contain one fluorine atom, all PFCs in the environment are anthropogenic (Giesy and Kannan, 2002). Rather than being lipophilic like many other persistent organic pollutants (POPs), several studies have suggested that PFCs are proteinophilic (Conder et al., 2008). PFOS behaves very differently from other POPs, having differences in intrinsic factors, such as surface activity, water solubility, non-measurable octanol/water coefficient values, and relatively low bioaccumulation potential. Fish from contaminated areas may be a significant source of dietary PFOS exposure (Berger et al., 2009). Various toxic endpoints have been measured in laboratory studies of PFCs. Observed effects of low doses of PFOS include decreased high density lipoprotein cholesterol (HDL) and changes in thyroid hormone levels in some animals. Recent studies have reported direct hormonal effects in fish exposed to PFCs in water. In addition to demonstrated direct toxic effects, PFCs might enhance cell membrane permeability of other pollutants (Wang et al., 2009). Mississippi River Pool 2 Mississippi River Pool 2 (Figure 1) extends from Lock & Dam No. 2 at Hastings, Minnesota, upstream 32.5 miles to Lock & Dam No. 1 (Ford Dam). The Mississippi confluence with the Minnesota River is at the upper reach of Pool 2 and the confluence with the St. Croix River is just below Lock & Dam No. 2. Within Pool 2, average annual flow is about 15,000 cubic feet per second (cfs). Pool 2 is a receivingwater for the largest publically-owned wastewater treatment plant (WWTP) in Minnesota, the Metropolitan WWTP, with an average discharge of 185 million gallons per day (286 cfs). The Eagles Point WWTP, with an average discharge of 4.2 mgd, is next to the 3M Company Cottage Grove facility at the lower end of Pool 2. The 3M facility discharges industrial process water and cooling water to the Mississippi River, with a combined flow of 8 mgd. 3M Company's Cottage Grove facility was a major manufacturer of PFOS from the 1950's to 2002. Although the facility discontinued production of PFOS, wastewater discharge from the facility continues to contain measurable PFOS concentrations. Discharge from the Metropolitan WWTP also has measureable PFCs because these chemicals have been included in a wide range of consumer and industrial products that have been disposed of in municipal and industrial wastewater systems. This report characterizes the levels of PFCs in Pool 2 and does not evaluate possible sources of PFCs to Pool 2. Perfluorochemicals in Mississippi River Pool 2 May 2013 1 Minnesota Pollution Control Agency Why this study? The MPCA is the state agency responsible for assessing and listing impaired waters for Minnesota, subject to federal U.S. Environmental Protection Agency (EPA) approval. MPCA rules specify that the MPCA shall list waters as impaired if the fish consumption advisory for a lake or river is more restrictive than a meal per week (Minn. R. 7050.0150, subp. 7). Pool 2 had been listed as impaired for mercury and PCBs in fish. Impairment caused by PFOS in fish was added in 2008, based on a one meal per month advisory for bluegill sunfish in 2007. Freshwater drum, first sampled in 2009, also had PFOS levels that warranted a meal per month advisory. The 2012 sample collection was a follow-up to the 2009 intensive study by the MPCA and the 2011 study by Cardno-Entrix for 3M. The MPCA and the Minnesota Department of Natural Resources (MDNR), with participation of the Minnesota Department of Health (MDH), have analyzed fish and river water for PFCs from Pool 2 since 2004. The 2009 study--which was designed together by 3M, MPCA, MDNR, and MDH--was the first year when the entire 32.5 mile reach of Pool 2 was sampled for PFCs in fish and water. Earlier collections focused on the lower five miles of Pool 2. Of the 367 fish analyzed from 2004 to 2009, 297 (81 percent) were collected in 2009. To characterize the spatial distribution of PFCs in 2009, Pool 2 was divided into four sections, with Section 1 at the most upstream end and Section 4 above the Hastings Dam (Figure 1). That same monitoring plan was followed in 2012. The 2011 study conducted by 3M was similar to the 2009 study design except that Pool 2 was divided into ten reaches (instead of four), three water samples were collected in each reach but not at the same location. Carp were not collected in 2011, but the other four species tested in 2009 were collected. Ten fish of each species were collected in each reach, for a total of 400 fish. Compared to the 2009 study, the 2011 sampling design collected 100 of each species instead of 60 of each species and reduced the percentage of fish collected in the lower reach (Section 4) from 25 percent (15/60 per species) to 18 percent (18/100 per species). The report by Cardno-Entrix and a second report by Exponent in November 2011 comparing the 2009 and 2011 results showed PFOS concentrations had declined from 2009 in the four tested fish species. Because of lower PFOS concentrations in 2011, the average PFOS concentration for the combined years dropped below the meal per month advisory threshold to an advisory level of one meal per week. MPCA did not delist Pool 2 as impaired for PFOS in fish based on the 2011 study because it was not sufficient to indicate a permanent downward trend in the PFOS concentrations and the sampling design in 2011 differed from the 2009 sampling design. Consequently, MPCA conducted the 2012 study, following the same sample design as 2009, to determine if the PFOS concentrations had indeed dropped to lower levels throughout Pool 2. In addition to collection of fish and water in 2012, the MPCA collected sediments and benthic macroinvertebrates in 2011 and 2012 to better understand the extent of the PFC contamination in Pool 2 and the possible direct source of PFOS in the fish. Unlike fish and water, sediments and insect nymphs living in the sediments are stationary and better represent the specific locations within the river. Freshwater drum and common carp, which had the highest PFOS concentrations in 2009, feed on sediments and benthic invertebrates more so than the other tested fish species. Perfluorochemicals in Mississippi River Pool 2 May 2013 2 Minnesota Pollution Control Agency Methods Fish collection Because of the long length of Pool 2, it was divided into four sections in the 2009 and 2012 studies for fish and water sampling (Figure 1). Average PFOS concentrations were then compared among sections as well as for all of Pool 2. Description of the four Pool 2 Sections: Section 1: Section 2: Section 3: Section 4: Approximately four river miles from Lock & Dam No. 1 (Ford Dam) to confluence with the Minnesota River. Ten river miles from the Minnesota River confluence to the lower channel to Hog Lake and Pig's Eye Lake, but not including Hog and Pig's Eye lakes; Metropolitan (Metro) wastewater treatment plant and Holman Field airport are in this section. Approximately 13.5 river miles include Hog Lake and Pig's Eye Lake as well as River, Baldwin, Mooers, and Spring lakes and Lower Grey Cloud Slough. The lower five river miles to Lock & Dam No. 2 in Hastings, which includes Spring Lake; Eagles Point wastewater treatment plant and 3M Cottage Grove facility are in this section. As in the 2009 study, the five target fish species in 2012 were bluegill sunfish (BGS), common carp (C), freshwater drum (FWD), smallmouth bass (SMB), and white bass (WHB). MDNR collected fish from the entire length of Pool 2 using electroshocking equipment. Collections occurred between July 30, 2012 and September 14, 2012. The beginning and end of each fish-collection run was geo-referenced (latitude and longitude). Each fish was identified by the section and run in which it was collected. Fish were wrapped in aluminum foil and immediately frozen. The frozen fish were labeled with a unique sample ID before shipping overnight to AXYS laboratory. Water collection Water samples were collected at 12 stations--3 stations per section--on September 24, 2012. Three samples at each station were collected as consecutive grab samples in 2 liter polyethylene bottles provided by AXYS. The bottle cap was removed after the bottle was completely immersed below the water surface and allowed to fill completely. The description of 12 water sample stations is as follows: Section 1 Water Station 1 2 3 Description Main channel at river mile 847 Old Minnesota River channel, near river mile 845.4 Main channel upstream of confluence with Minnesota River at river mile 844 2 4 Main channel near boat launch at river mile 842 5 Main channel adjacent to Holman Field at river mile 837 6 Bay south of Pigs Eye Lake near river mile 834 3 7 Main channel near boat launch; downstream from St. Paul Park Refinery at river mile 829.5 8 Main channel upstream of Island 112 at river mile 827.5 9 Near Spring Lake at river mile 821 4 10 Main channel, downstream of Grey Cloud Slough at river mile 819 11 Near shoreline, downstream of 3M Cottage Grove discharge, near river mile 817.5 12 Main channel, upstream of Lock & Dam No. 2 at river mile 816 Perfluorochemicals in Mississippi River Pool 2 May 2013 3 Minnesota Pollution Control Agency Sediment and benthic macroinvertebrate collection Sediment samples were collected with a modified Hongve surface-sediment drop corer, with a rubber ball valve at the top (modification by Harold Wiegner, MPCA). Before each collection the corer and stainless steel spatula were washed with methanol (tested and provided by AXYS lab). The top 5 cm of sediment were collected and dispensed into 250 mL polyethylene jars (provided by AXYS lab). Jars were labeled with Site ID, date, and time. The sediment sample was stirred with the spatula and a sub-sample was placed in a 250 mL glass jar (provided by Pace Laboratories) for total organic carbon. All samples were kept on ice in a cooler. Benthic macroinvertebrates (invertebrates) were collected with a Petite Ponar sampler, which grabs a sediment sample with an area of 6 x 6 inches (152 x 152 mm). The Ponar was dropped from the bow of the boat multiple times as needed to collect a sufficient mass of invertebrates. The sediment sample was deposited into a stainless-steel wash screen (356 x 598 x 192 mm; 500 m mesh) and rinsed with river water as needed to expose invertebrates. All benthic invertebrates known to live in the sediments (excluding species that lived on or near sediment surface such as amphipods) were removed from the sediments with stainless-steel forceps and placed in a polyethylene 250 mL jar (provided by AXYS lab). The number of Ponar grabs and general description of invertebrates were noted in the field notebook. Invertebrate samples were kept on ice in a cooler. At the end of a sample day, all sediment and invertebrate samples were placed in a freezer until they were shipped to the laboratory. Laboratory analysis AXYS Analytical Services Ltd analyzed all samples for PFCs. Empty coolers were shipped by AXYS to the MPCA for fish samples and coolers with bottles were shipped for other samples. The whole fish shipped to AXYS were measured for length and weight; also, gender and age were recorded. AXYS homogenized fish fillets, with scales off and skin on, which is the protocol used for fish contaminant analysis when results are used for fish consumption advisories. The USEPA has not yet approved analytical methods for PFCs in matrices (except for drinking water); however, all laboratories conducting PFC analysis on environmental samples use a method of liquid chromatography-electrospray tandem mass spectrometry (LC-MS/MS). Sample homogenization, extraction, and clean-up steps can vary among laboratories. AXYS describes their aqueous method as, AXYS Method MLA-060: Analytical Procedure for the Analysis of Perfluorinated Organic Compounds in Aqueous Samples by LC-MSIMS and their tissue method as, AXYS Method MLA-043: Analytical Procedure for the Analysis of Perfluorinated Organic Compounds in Tissue Samples by LC-MS/MS. The reporting level is the limit of quantitation (LOQ), defined as the lowest non-zero calibration standard having accuracy of 100 30 percent. Quality assurance In addition to the multiple level quality assurance protocol at AXYS, William Scruton, Quality Assurance Coordinator for MPCA's Environmental Analysis and Outcomes Division, reviewed all data packages from AXYS and identified any results needing to be qualified as an estimate if all QA requirements were not met. Laboratory blank and matrix spike results for each batch (Work Group) and the qualified analytes are tabulated, along with all PFC results, in Appendix A (fish and water) and Appendix B (sediment and benthic macroinvertebrates). In the 2009 study of PFCs in fish and water from Pool 2 (MPCA 2010), water samples from all 12 stations and 30 randomly-selected fish samples were analyzed by 3M Environmental Laboratory. The 3Msponsored study in 2011 also split samples of water and fish with AXYS. The high degree of data correlation between the two labs provided confidence in the quality of the results. Because of the positive results from 2009 and 2011, split samples with the 3M Environmental Laboratory were not included in the 2012 study. Perfluorochemicals in Mississippi River Pool 2 May 2013 4 Minnesota Pollution Control Agency Results and discussion PFOS concentrations in water, fish, sediment, and benthic macroinvertebrates are presented in this section. Laboratory results for all 13 analyzed PFCs are tabulated in Appendix A for fish and water and Appendix B for sediment and benthic macroinvertebrates. Surface water PFOS concentrations in water were similar in 2009 and 2012 (Table 1). Reporting limits were approximately 5 ng/L in both years. PFOS concentrations were consistently below the detection limit in the upper Stations, 1 to 5. In 2009, measureable PFOS levels at Stations 6, 7, and 8 averaged 7.7, 10.3, and 8.5 ng/L. In 2012, PFOS levels at the three Stations were below the reporting limit, with the exception of one sample at Station 8 (19.8 ng/L). Stations 9 and 10 were below the reporting limit in both years, with the exception of a detectable PFOS concentration in one sample from Station 9 in 2012 (6.09 ng/L). The most noteworthy results were in Stations 11 and 12. Station 11 is immediately downstream of the 3M Cottage Grove Center and the East Cove, which had been dredged in late 2011 to remove PFCcontaminated sediments. Station 12 is farther downstream (Figure 1). PFOS concentrations were similar among the replicates at each Station within each year. The coefficients of variation (standard deviation/mean) were 5.7 percent and 4.4 percent for Station 11 in 2009 and 2012, and 26.4 percent and 5.8 percent for Station 12 in 2009 and 2012. The mean PFOS concentrations increased from 2009 to 2012, from 90.1 ng/L to 149 ng/L at Station 11 and 15.2 ng/L to 24.4 ng/L at Station 12. These were increases of 65 percent and 61 percent, respectively. The 2011 study results for PFOS in water concur with the results from 2012. The most downstream station in the 2011 study was at approximately the same location as Station 11. The water sample, collected on 11 August 2011 had a PFOS concentration of 136 ng/L. Results from all years indicate substantially higher PFOS concentrations in water downstream of the 3M facility compared to upstream. The increased PFOS concentrations in 2011 and 2012 could have been caused by greater inputs to Pool 2 or inputs could have remained the same, but there was less dilution because of lower water volume (i.e., low river flows). To test the possibility of change in dilution, the water levels at the time of sampling were compared. The closest water level gaging station is U.S. Army Corp of Engineer's CP2 station at South St. Paul. Water level at CP2 was actually very similar on July 2, 2009 and September 24, 2012, but higher on August 11, 2011 (Figure 2). The 24-hour average water levels for the three sample days were 287.2 ft. in 2009, 289.5 ft. in 2011, and 287.1 ft. in 2012. Water level in 2011 was over two feet higher than in 2009 and 2012. Therefore, the water level results do not support an argument that dilution was the reason for differences among years. Fish The goal was to collect 15 of each species in each section, for a total of 60 per species. That goal was met for all but the white bass. In Sections 1 and 4, 13 white bass were collected. Fish-collection runs are shown in Figure 3. Appendix A lists the georeferenced start and stop of each run and the number of fish in each species captured in each run. All results, from 2004 to 2012, were combined for the initial descriptive statistics for the five target species (Table 2). Four of the species have over 200 individual fish measurements. Common carp was the exception with 125 fish tested, mostly because that species was not included in the 2011 study by 3M. Of the 296 fish analyzed for PFCs in 2012, only four fish had PFOS concentrations less than the reporting limit (1.0 ng/g). The highest PFOS concentration, 6,160 ng/g, was measured in a carp from Section 4 in 2012. The next highest, 5,150 ng/g, was measured in a smallmouth bass in 2006. The similarity of the median with the geometric mean rather than the Perfluorochemicals in Mississippi River Pool 2 May 2013 5 Minnesota Pollution Control Agency arithmetic mean indicates the distributions of PFOS concentrations are log-normally distributed. White bass had the lowest maximum PFOS concentration, but they had a median concentration approximately double the median of the other four species. Descriptive statistics by species and year are summarized in Table 3 on page 12. The statistics for all years are presented at the top row for each species. The left side of the table shows values for all of Pool 2, while the right side shows values for the Lower Pool 2. Lower Pool 2 is Section 4 in 2009 and 2012, and Reaches 9 and 10 in 2011. Prior to 2009 all fish were collected in the Lower Pool 2; therefore, the statistics for years 2004-2008 are the same in both sides of the table. Consequently, comparisons among years within a species are best done only for the Lower Pool 2. Smallmouth bass (SMB) had the longest period of record--seven years--followed by bluegill sunfish (BGS), six years, and white bass (WHB), five years. Freshwater drum (FWD) were first collected in 2009. Comparing annual medians in the Lower Pool 2 for each species shows a general decline over time, especially in the most recent two years. Freshwater Drum shows a striking drop from 401 ng/g in 2009 to 22 ng/g and 23 ng/g in 2011 and 2012. Common carp (C) had the lowest median, 15 ng/g, in 2012 and the highest maximum PFOS concentration, 6,160 ng/g. The distributions of PFOS concentration within species over time are illustrated in box-whisker plots (see diagram at right), which show the median, the interquartile range and outliers (Figure 4, page 17). PFOS concentrations are on a log-10 scale to improve the symmetry of the distributions. Smallmouth bass did not show a trend between 2004 and 2009, but with the addition of 2011 and 2012 there appears to be a downward trend in the PFOS concentrations (Figure 4a). The three years of PFOS in common carp (2005, 2009, and 2012) show a downward trend as well (Figure 4b). The downward trend is not as clear for freshwater drum (Figure 4c); there is a big drop in median PFOS concentration from 2009 to 2011 and 2012, but the ranges of PFOS concentrations in FWD remain similar among the three years. Comparing the distributions of PFOS by species and section for 2009 and 2012, there was a general shift to the left to lower PFOS concentrations in 2012 (Figure 5). The shifts to the left increased from Section 1 to Section 4 for all species (quantile plots from top to bottom). The exception to this pattern is carp, which shows a large decline in PFOS concentrations in Section 1 and less of a shift in Sections 2 and 3. There was less of a difference among Sections for any given species in 2012 than in 2009, when Section 4 PFOS concentrations were sharply higher than in the three upstream sections. Despite the shift to lower concentrations, all species show the higher PFOS concentrations in Section 4. Bluegill sunfish, carp, and freshwater drum continued to have a few high PFOS concentrations in Section 4. Looking at the specific fish-collection runs in Section 4 from 2012 shows the high PFOS concentrations (> 400 ng/g) for carp and freshwater drum were in Run 2, located downstream of the 3M Cottage Grove Center (Figure 6; see Figure 3b for a map of the runs in Section 4). The high outlier for bluegill sunfish was in Run 1 (1,020 ng/g), immediately upstream of the 3M center. Bioaccumulation factors and site-specific water quality criterion The MPCA followed EPA guidance and Minnesota rules in calculating bioaccumulation factors (BAFs) and site-specific water quality criterion for PFOS. Water was sampled in each river section at three stations, which were averaged to calculate the PFOS water concentration for that section. Fish were collected from multiple sites within each river section. BAFs for PFOS were calculated for each fish fillet using the PFOS tissue concentration from that fillet divided by the average PFOS water concentration reported from the river section where the fish was captured. PFOS water concentrations in the upper 3 Sections of Pool 2 were predominantly less than the reporting level. For water concentrations below the Perfluorochemicals in Mississippi River Pool 2 May 2013 6 Minnesota Pollution Control Agency reporting level, the reporting level was used. The individual BAFs were then combined as a geometric mean (geomean) for each species. (A geometric mean is the nth root (where n is the count of numbers) of the product of the numbers or is alternatively defined as the antilog of the average of logtransformed values.) The final BAF is a geomean of all species geomeans. The BAFs are applied to the equation for calculating a fish consumption criterion (fCC), which is the site-specific water criterion. The equation is RfD + BW + K = IW + (CR BAF) where, RfD = reference dose, 0.00008 mg/kg/d BW = body weight, 70 kg K = exposure fraction attributed to water and fish consumption, 0.2 IW = incidental ingestion of water, 0.01 L/d CR = fish consumption rate, 0.030 Kg/d This calculation had been done in previous years. An fCC for PFOS in Pool 2 was calculated as 6 ng/L in 2007 using a small dataset (STS Consultants, 2007). Calculations from the 2009 study resulted in an fCC of 7 ng/L. Lower fish tissue concentrations in 2012, while water concentrations remained about the same as in 2009, resulted in lower BAFs and subsequently a higher calculated fCC of 14 ng/L. Sediments Sediment sampling began in late November 2011. The top 5 cm of surface sediments were collected at 50 sites in Pool 2 for PFCs analysis (Figure 7). Another round of sampling occurred in May 2012. A third and final round was completed in October 2012. PFOS concentrations in sediment were measured in dry samples. AXYS provided percent moisture content of the samples, allowing for calculation of wet weight concentrations. Total organic carbon was measured in the sediment samples by PACE Laboratories and presented as fraction organic carbon (fOC). Moisture content of sediment samples ranged from 21 percent to 62 percent, with a median of 37.5 percent (Table 4). The ranges of moisture content were very similar among the three periods of sample collection and were not significantly different (Kruskal-Wallis One-way ANOVA test statistic: 0.016: p = 0.992). The same was true for fraction organic carbon (Kruskal-Wallis test statistic: 1.594; p = 0.451). The median fOC for the combined samples was 1.0 percent. fOC was less than 2.6 percent in all but one sample; the site on the left bank of river mile 836 in Section 2 (upstream of the Metro WWTP) had an fOC of 12.8 percent. Sediment PFOS concentrations ranged from less than the reporting limit (< 0.188 ng/g-dw) to 80.2 ng/gdw, with a median of 0.5 ng/g-dw. One-half the reporting limit was substituted for the eight sediment samples that had dry weight PFOS concentrations less than the reporting limit to calculate the arithmetic and geometric means of dry weight concentrations, but were not used to calculate wet weight concentrations. Field replicates were collected at five sites; three had duplicate sediment samples and two had triplicate samples (Table 5). The relative standard deviation (RSD = Std. Dev *100/Average) is shown for the measurements in each set of replicates. RSD was reduced when dry weight PFOS concentrations were converted to wet weight. RSD was reduced in four of the five replicate sets when PFOS was converted to per unit organic carbon (ng/g - OC). The exception was the set of triplicate samples from the Metro WWTP discharge channel (MC_1, MC_2, and MC_3). The fOC were sufficiently different among the replicates in the channel to cause an increase in the RSD when PFOS was converted to ng/g-OC. Perfluorochemicals in Mississippi River Pool 2 May 2013 7 Minnesota Pollution Control Agency One site at river mile 828 (Saint Paul Park area) was sampled in November 2011 and in May 2012. The dry weight PFOS concentrations in sediment were 0.33 ng/g and 0.53 ng/g. Percent moisture for the respective samples was 39 percent and 62 percent, resulting in wet weight PFOS concentrations of 0.198 and 0.200 ng/g. The fraction of organic carbon (foc) was 1.17 percent and 2.03 percent, giving PFOS per OC of 27.9 and 25.9 ng/g. The similarity of the PFOS concentrations provided added confidence in the results for sediments. The highest sediment and invertebrate PFOS concentrations were in samples collected downstream of the 3M Cottage Grove Center, near the East Cove. In November 2011, three sediment samples (Site ID: 517Sed) were collected in that area and another set of duplicate samples were collected in October 2012 (Site ID: K Sed) (Table 5). One of the triplicate samples in 2011 was 80 ng/g-dw, while the other two were 33 ng/g-dw and 41 ng/g-dw. The high concentration also had the highest organic carbon content (foc). Consequently, weighted by organic carbon, the three PFOS concentrations were more similar. The following fall sampling, in October 2012, found much lower sediment PFOS concentrations (8.6 and 6.4 ng/g-dw), although still much higher than seen at any other station in Pool 2. The sediment and invertebrate samples in 2012 were collected in the same vicinity as collections in 2011 but not precisely in the same location as the previous fall, which may explain the differences in PFOS concentrations between sampling events. The other triplicate set of samples was collected in May 2012 in the Metro WWTP discharge channel. The dry weight PFOS concentrations were 0.581, 0.672, and 0.905 ng/g. Most of the PFOS concentrations in the main channel of Pool 2, upstream and downstream of the discharge, were slightly below those sediment concentrations. Two stations immediately downstream of the Metro Channel had somewhat higher concentrations: 1.58 and 2.07 ng/g-dw in the sediment. A station across the channel from the first downstream station had sediment concentration of less than 0.195 ng/g-dw. Benthic invertebrates Benthic invertebrates were sufficiently abundant at 39 of the 50 sediment stations for PFC analysis. Sediments in the upper half of Pool 2 were sandy and had few invertebrates. The collected invertebrates were primarily larvae of Chironomids (non-biting midges) and Hexagenia (mayflies). PFOS concentrations ranged from 1.7 ng/g-ww to 684 ng/g-ww, with a median of 11.9 ng/g-ww (Table 4). Only two samples had PFOS concentrations greater than 50 ng/g-ww (194 ng/g and 684 ng/g) and they were both collected immediately downstream of the 3M Cottage Grove Center. When PFOS concentrations in benthic invertebrates are compared alongside the PFOS concentrations in sediments, they both show the highest concentrations in the lower section of Pool 2, downstream of the 3M Cottage Grove Center (Figure 8). The invertebrate PFOS concentration at this site in 2011 was the highest measurement among all benthic macroinvertebrate samples. Similar to the relative PFOS concentrations in sediments at these sites, the invertebrate PFOS concentration was lower in 2012 than was measured in 2011, but much higher than any other station in Pool 2. As noted above, different sample locations could explain slight differences between sampling. The relationship between invertebrate and sediment PFOS concentrations is a significant positive correlation when the relatively high concentrations downstream of 3M are included (Figure 9; logtransformed slope: p < 0.000). Excluding the sediment PFOS concentrations greater than 5 ng/g, there appears to be a positive relationship, but a least square regression indicates the slope is not significantly different from zero whether linear or log-transformed (linear: p = 0.77; log-log: p = 0.121). PFOS concentrations in invertebrates appeared to be higher at low organic carbon content of sediments, but they were not significantly correlated (Pearson r = 0.082 or r = -0.387 when outliers excluded). Perfluorochemicals in Mississippi River Pool 2 May 2013 8 Minnesota Pollution Control Agency Conclusions Compared to 2009 PFOS concentrations in the targeted fish species declined in the last two years (2011 and 2012) of monitoring in Pool 2. PFOS concentrations in water from the Lower Pool 2 did not decline. Consequently, the calculated bioaccumulation factors were lower, which led to a higher site-specific water quality criterion for PFOS: from 7 ng/L calculated from 2009 results to 14 ng/L calculated from 2012 results. Despite the average decline for all of Pool 2, PFOS concentrations in all tested media--fish, benthic macroinvertebrates, sediment, and water--remain high in the Lower Pool 2, downstream of the 3M Cottage Grove Center. References Berger U., Glynn A., Holmstrom K.E., Berglund M., Ankarberg E.H., Tornkvist A. (2009) Fish consumption as a source of human exposure to perfluorinated alkyl substances in Sweden - Analysis of edible fish from Lake Vattern and the Baltic Sea. Chemosphere 76:799-804. DOI: 10.1016/j.chemosphere.2009.04.044. Cardno-Entrix (2011) Mississippi River Pool 2 PFOS Assessment - Fish Tissues and Water - Summer 2011. Prepared for 3M Company. November 2011. Project No. 70784004. Conder J.M., Hoke R.A., De Wolf W., Russell M.H., Buck R.C. (2008) Are PFCAs bioaccumulative? A critical review and comparison with regulatory lipophilic compounds. Environmental Science & Technology 42:995-1003. DOI: 10.1021/es070895g. Exponent (2011) Review and Evaluation of 2011 Report on Mississippi River Pool 2 PFOS Assessment of Fish and Water. Prepared for 3M Company. November 2011. Giesy J.P., Kannan K. (2001) Global distribution of perfluorooctane sulfonate in wildlife. Environmental Science & Technology 35:1339-1342. DOI: 10.1021/es001834k. Giesy J.P., Kannan K. (2002) Perfluorochemical surfactants in the environment. Environmental Science & Technology 36:146A-152A. Houde M., Martin J.W., Letcher R.J., Solomon K.R., Muir D.C.G. (2006) Biological monitoring of polyfluoroalkyl substances: A review. Environmental Science & Technology 40:3463-3473. DOI: 10.1021/es052580b. MPCA. (2010) Mississippi River Pool 2 Intensive Study of Perfluorochemicals in Fish and Water: 2009. 8 March 2010. STS Consultants. (2007) Surface Water Quality Criterion for Perfluorooctane Sulfonic Acid Minnesota Pollution Control Agency. Wang T., Wang Y.W., Liao C.Y., Cai Y.Q., Jiang G.B. (2009) Perspectives on the Inclusion of Perfluorooctane Sulfonate into the Stockholm Convention on Persistent Organic Pollutants. Environmental Science & Technology 43:5171-5175. DOI: 10.1021/es900464a. For more information on this report contact: Bruce Monson Research Scientist Minnesota Pollution Control Agency 520 Lafayette Road N St. Paul, MN 55155 Phone: 651-757-2579 Perfluorochemicals in Mississippi River Pool 2 May 2013 9 Minnesota Pollution Control Agency Minnesota Pollution Control Agency PFCs http://www.pca.state.mn.us/cleanup/pfc/index.html Minnesota Department of Health PFCs http://www.health.state.mn.us/divs/eh/hazardous/topics/pfcs/index.html Minnesota Department of Health Fish Consumption Guidelines http://www.health.state.mn.us/divs/eh/fish/index.html Perfluorochemicals in Mississippi River Pool 2 May 2013 10 Minnesota Pollution Control Agency Tables Table 1. PFOS concentrations (ng/L) in water samples collected at the same twelve stations in 2009 and 2012; A, B, and C refer to consecutive grab samples collected at each station; in 2012 two samples from Stations 1 and 5 were used for laboratory matrix spikes. Station A 1 < 5.12 2 < 5.08 3 < 5.15 4 < 5.03 5 < 5.07 6 7.1 7 10.5 8 8.74 9 < 5.05 10 < 5.07 11 84.4 12 15.1 2 July 2009 B C < 5.14 < 5.08 < 5.10 < 5.02 < 5.03 < 4.97 < 5.09 < 5.04 < 5.11 < 4.93 7.8 8.24 10.5 9.91 8.4 8.4 < 5.04 < 5.17 < 5.05 < 4.97 91.3 94.5 19.2 11.2 Mean < DL < DL < DL < DL < DL 7.7 10.3 8.5 < DL < DL 90.1 15.2 24 September 2012 A B C Mean < 4.99 < DL < 4.84 < 4.83 < 4.91 < DL < 4.90 < 4.92 < 4.93 < DL < 5.00 < 4.99 < 4.85 < DL < 5.06 < DL < 4.99 < 4.97 < 5.08 < DL < 5.02 < 4.88 < 4.88 < DL < 4.92 < 4.81 19.8 < DL? < 4.89 < 5.28 6.09 < DL? < 4.54 < 5.18 < 5.29 < DL 150 142 155 149 24.2 25.9 23.1 24.4 Table 2. Descriptive statistics for PFOS (ng/g -ww) in the five target fish species; combined results for 2004-2012 Species Bluegill Sunfish (BGS) Common Carp (C) Freshwater Drum (FWD) Smallmouth Bass (SMB) White Bass (WHB) N 230 125 220 246 219 Minimum 3.57 0.995 3.17 2.37 13.0 Statistic Arithmetic Maximum Mean 2097 83.0 6160 107.0 3580 113.1 5150 99.7 1860 80.9 Geometric Mean 31.4 21.2 36.9 40.4 62.5 Median 28.6 23.6 34.1 32.8 59.7 Perfluorochemicals in Mississippi River Pool 2 May 2013 11 Minnesota Pollution Control Agency Table 3. PFOS concentrations (ng/g -ww) for each fish species and year from all of Pool 2 (left) and only Lower Pool 2--Section 4 (right); the top row of each species is for all years; results from the study in 2011 used lower Reach 9 and Reach 10 to represent the Lower Pool 2; in 2004-2008 all fish were collected in the Lower Pool 2. SpeciesYear BGS 2006 2007 2008 2009 2011 2012 C 2005 2009 2012 FWD 2009 2011 2012 SMB 2004 2005 2006 2008 2009 2011 2012 WHB 2004 2005 2009 2011 2012 Mississippi Pool 2 (all reaches) Arith. N Min Max Mean Median 230 3.6 2097 83 29 6 30 709 249 195 2 2001 2097 2049 2049 5 102 317 173 157 57 8.3 1350 110 45 100 3.6 392 36 18 60 6.8 1020 46 26 125 1.00 6160 107 24 5 66 420 216 175 60 4.7 1340 77 28 60 1.00 6160 127 11 220 3.2 3580 113 34 60 4.8 3580 229 63 100 3.2 607 46 30 60 3.9 1580 109 25 246 2.4 5150 100 33 4 92 985 528 517 6 122 336 201 163 11 19 5150 793 178 5 49 380 162 120 60 13 612 94 41 100 4.9 757 39 30 60 2.4 192 35 28 219 13 1860 81 60 2 137 139 138 138 5 84 1860 559 240 60 38 764 97 75 96 13 394 64 58 56 20 153 48 42 Lower Pool 2 (Section 4) Arith. N Min Max Mean Median 61 4.5 2097 204 46 6 30 709 249 195 2 2001 2097 2049 2049 5 102 317 173 157 15 32 1350 260 53 18 4.5 305 63 26 15 11 1020 98 28 35 6.0 6160 315 56 5 66 420 216 175 15 8.7 1340 224 87 15 6.0 6160 438 15 48 4.8 3580 356 33 15 24 3580 739 401 18 4.8 607 81 22 15 6.0 1580 347 23 69 7.3 5150 252 83 4 92 985 528 517 6 122 336 201 163 11 19 5150 793 178 5 49 380 162 120 15 42 612 260 210 13 9.5 757 109 44 15 8.1 192 59 47 50 27 1860 144 78 2 137 139 138 138 5 84 1860 559 240 15 44 764 159 88 15 41 394 92 71 13 27 153 59 46 Perfluorochemicals in Mississippi River Pool 2 May 2013 12 Minnesota Pollution Control Agency Table 4. Summary statistics for sediment and invertebrate measurements Statistic Measurement Arithmetic Geometric N Minimum Maximum Mean Mean Median Percent Moisture 58 20.8 61.9 38.95 37.57 37.5 Fraction Organic Carbon, fOC (%) 58 Sediment PFOS dry wt. (ng/g) 58 0.10 <0.188 12.8 1.30 0.90 1.00 80.2 3.405 0.548 0.497 Sediment PFOS wet wt. (ng/g)* 50 0.072 32.6 1.848 0.423 0.310 Sediment PFOS per OC. (ng/g)* 50 2.3 2,823 235.3 66.5 56.1 Invertebrate PFOS wet wt. (ng/g) 39 1.65 684 35.71 13.41 11.9 *Eight dry weight sediment PFOS concentrations were less than the reporting limit, precluding calculation of wet weight concentrations for those eight. Table 5. Replicates of sediment PFOS concentrations with matching invertebrate PFOS concentrations and BSAF Date 11/23/11 15:00 11/23/11 15:10 11/23/11 15:18 10/16/12 15:06 10/16/12 15:10 10/16/12 11:20 10/16/12 11:25 10/16/12 13:55 10/16/12 13:58 05/22/12 14:00 05/22/12 14:05 05/22/12 14:15 05/23/12 13:55 11/30/11 13:10 Sample ID_Sed 517 SED1 517 SED2 517 SED3 RSD K SED K SED FD RSD G SED G SED FD RSD M SED M SED FD RSD MC_1_SED MC_2_SED MC_3_SED RSD P2_525_SED 525 SED RSD Sed_PFOS_ ng/g_dw 80.2 33.3 41.1 48.8 8.58 6.38 20.8 0.601 0.860 25.1 0.203 0.366 40.5 0.905 0.672 0.581 23.2 0.525 0.330 33.1 sed_% Moisture 59.3 51.1 52.9 7.9 45.8 43.5 3.6 40.6 45.9 8.7 25.9 28.6 7.0 44.6 32.2 26.8 26.4 61.9 39.2 31.8 Sed_PFOS ng/g_ww 32.64 16.28 19.36 38.2 4.65 3.60 17.9 0.357 0.465 18.6 0.150 0.261 38.1 0.501 0.456 0.425 8.3 0.200 0.198 0.6 foc 2.84% 1.75% 1.62% 32.4 0.98% 0.70% 24.1 0.95% 1.13% 11.9 0.36% 0.66% 41.1 2.17% 1.67% 0.59% 54.6 2.03% 1.17% 37.8 Sed_PFOS ng/g _OC 2,824 1,903 2,537 19.5 874.2 916.7 3.4 63.0 76.1 13.4 56.2 55.8 0.6 41.8 40.2 98.6 55.2 25.9 27.9 5.1 Perfluorochemicals in Mississippi River Pool 2 May 2013 13 Minnesota Pollution Control Agency Figures Figure 1. Pool 2 showing river miles between Lock & Dam No. 1 at river mile 847.6 to Lock & Dam No. 2 at river mile 815.6 (~32.5 miles); shows four sections and PFC water stations for 2009 and 2012 Perfluorochemicals in Mississippi River Pool 2 May 2013 14 Minnesota Pollution Control Agency a 690 689.5 689 Elevation (feet) 688.5 688 687.5 687 686.5 0 4 8 12 16 20 24 Hour b 1-Jul-09 10-Aug-11 24-Sep-12 Figure 2. Water levels at Mississippi River South St. Paul: (a) record between 1 July 2009 and 1 October 2012; (b) hourly water levels on sampling days (http://www.mvp-wc.usace.army.mil/dcp/SSPM5.html) Perfluorochemicals in Mississippi River Pool 2 May 2013 15 Minnesota Pollution Control Agency a b Figure 3. Fish-collection runs in Pool 2: a. entire Pool 2 showing reaches and runs; b. runs in Section 4 Perfluorochemicals in Mississippi River Pool 2 May 2013 16 Minnesota Pollution Control Agency a b c 1000 PFOS (ng/g) 100 10 2004 2005 2006 2008 2009 2011 2012 Year 2004 2005 2006 2008 2009 2011 2012 Year 2004 2005 2006 2008 2009 2011 2012 Year Figure 4. Box-whisker plots of PFOS concentrations in Lower Pool 2 by year for (a) smallmouth bass, (b) carp, and (c) freshwater drum Perfluorochemicals in Mississippi River Pool 2 May 2013 17 Minnesota Pollution Control Agency Fraction of Data Fraction of Data Species Section BGS C FWD SMB WHB 1.0 0.9 0.8 0.7 0.6 1 0.5 0.4 0.3 0.2 0.1 01..00 0.9 0.8 0.7 0.6 2 0.5 0.4 0.3 0.2 0.1 01..00 0.9 0.8 0.7 0.6 3 0.5 0.4 0.3 0.2 0.1 10..00 0.9 0.8 0.7 0.6 0.5 4 0.4 0.3 0.2 0.1 0.0 1 10 100 1000 1 10 100 1000 1 10 100 1000 1 10 100 1000 1 10 100 1000 PFOS (ng/g) PFOS (ng/g) PFOS (ng/g) PFOS (ng/g) PFOS (ng/g) Figure 5. Species-Section matrix showing distributions of PFOS in 2009 (red) and 2012 (blue); horizontal mid-line is the median; vertical lines are PFOS thresholds of 40 ng/g and 200 ng/g Fraction of Data Fraction of Data Perfluorochemicals in Mississippi River Pool 2 May 2013 18 Minnesota Pollution Control Agency PFOS (ng/g) 1000 100 10 123456 RUN Figure 6. PFOS concentrations by fish-collection runs in Section 4 (2012) Species BGS C FWD SMB WHB Perfluorochemicals in Mississippi River Pool 2 May 2013 19 Minnesota Pollution Control Agency Figure 7. PFOS concentrations (dry weight) in surface sediments collected between November 2011 and November 2012; concentrations are color coded and insert map shows measured concentrations in Section 4 of Pool 2 Perfluorochemicals in Mississippi River Pool 2 May 2013 20 Minnesota Pollution Control Agency Figure 8. PFOS concentrations in sediment (ng/g dry weight) and benthic macroinvertebrates (ng/g wet weight) Perfluorochemicals in Mississippi River Pool 2 May 2013 21 Minnesota Pollution Control Agency Inv PFOS (ng/g-ww) 100 10 1 0.1 1.0 10.0 Sed PFOS (ng/g - ww) Figure 9. Relationship between invertebrate and sediment PFOS concentrations (both wet weight and log-scale) Perfluorochemicals in Mississippi River Pool 2 May 2013 22 Minnesota Pollution Control Agency Appendix A: Fish and Water Data Appendix A-1. PFCs results for fish (ng/g - wet weight)............................................................................. 24 Appendix A-2. PFCs results for water (ng/L) ............................................................................................... 34 Appendix A-3. QA Results for Fish Samples: lab blanks and matrix spikes by work group ........................ 36 Appendix A-4. QA Results for Water Samples: lab blanks and matrix spikes by work group..................... 39 Appendix A-5. Fish sample identification ................................................................................................... 40 Appendix A-6. Georeferences for fish-collection runs................................................................................ 51 Appendix A-7. Georeferences for water sample stations........................................................................... 54 Perfluorochemicals in Mississippi River Pool 2 May 2013 23 Minnesota Pollution Control Agency Appendix A-1. PFCs results for fish (ng/g - wet weight) CLIENT ID 123076 123077 123078 123079 123080 123081 123082 123083 123084 123085 123086 123087 123088 123089 123090 123091 123092 123093 123094 123095 123096 123097 123098 123099 123100 123101 123102 123103 123104 123105 WORK GROUP WG40901 WG40901 WG40901 WG40901 WG40901 WG40901 WG40901 WG40901 WG40901 WG40901 WG40901 WG40901 WG40901 WG40901 WG40901 WG40901 WG40901 WG40901 WG40901 WG40902 WG40902 WG40902 WG40902 WG40902 WG40902 WG40902 WG40902 WG40902 WG40902 WG40902 Sample (g - wet) 2.00 2.05 2.05 2.03 2.04 2.00 2.00 2.01 2.05 2.04 2.00 2.07 2.00 2.05 2.04 2.05 2.02 2.05 2.01 2.02 2.01 2.03 2.04 2.01 2.00 2.03 2.00 2.05 2.05 2.01 PFBA < 0.500 < 0.488 < 0.488 < 0.493 < 0.490 < 0.500 < 0.500 < 0.498 < 0.488 < 0.490 < 0.500 < 0.483 < 0.500 < 0.488 < 0.490 < 0.488 < 0.495 < 0.488 < 0.498 < 0.495 < 0.498 < 0.493 < 0.490 < 0.498 < 0.500 < 0.493 < 0.500 < 0.488 < 0.488 < 0.498 PFPeA < 0.500 < 0.488 < 0.488 < 0.493 < 0.490 < 0.500 < 0.500 < 0.498 < 0.488 < 0.490 < 0.500 < 0.483 < 0.500 < 0.488 < 0.490 < 0.488 < 0.495 < 0.488 < 0.498 < 0.495 < 0.498 < 0.493 < 0.490 < 0.498 < 0.500 < 0.493 < 0.500 < 0.488 < 0.488 < 0.498 PFHxA < 0.500 < 0.488 < 0.488 < 0.493 < 0.490 < 0.500 < 0.500 < 0.498 < 0.488 < 0.490 < 0.500 < 0.483 < 0.500 < 0.488 < 0.490 < 0.488 < 0.495 < 0.488 < 0.498 < 0.495 < 0.498 < 0.493 < 0.490 < 0.498 < 0.500 < 0.493 < 0.500 < 0.488 < 0.488 < 0.498 PFHpA < 0.500 < 0.488 < 0.488 < 0.493 < 0.490 < 0.500 < 0.500 < 0.498 < 0.488 < 0.490 < 0.500 < 0.483 < 0.500 < 0.488 < 0.490 < 0.488 < 0.495 < 0.488 < 0.498 < 0.495 < 0.498 < 0.493 < 0.490 < 0.498 < 0.500 < 0.493 < 0.500 < 0.488 < 0.488 < 0.498 PFOA < 0.500 < 0.488 < 0.488 < 0.493 < 0.490 < 0.500 < 0.500 < 0.498 < 0.488 < 0.490 < 0.500 < 0.483 < 0.500 < 0.488 < 0.490 < 0.488 < 0.495 < 0.488 < 0.498 < 0.495 < 0.498 < 0.493 < 0.490 < 0.498 < 0.500 < 0.493 < 0.500 < 0.488 < 0.488 < 0.498 PFNA < 0.500 < 0.488 < 0.488 < 0.493 < 0.490 < 0.500 < 0.500 < 0.498 < 0.488 < 0.490 < 0.500 < 0.483 < 0.500 < 0.488 < 0.490 < 0.488 < 0.495 < 0.488 < 0.498 < 0.495 < 0.498 < 0.493 0.781 < 0.498 0.715 0.717 < 0.500 0.671 < 0.488 < 0.498 PFDA < 0.500 < 0.488 0.587 < 0.493 < 0.490 < 0.500 < 0.500 3.2 2.37 < 0.490 1.46 1.85 1.53 1.73 2.45 2.23 4.1 1.83 0.935 0.615 0.586 1.2 2.2 0.567 1.53 0.669 1.44 1.26 1.75 1.77 PFUnA < 0.500 < 0.488 < 0.488 < 0.493 < 0.490 < 0.500 < 0.500 1.48 1.32 < 0.490 0.603 0.724 0.735 0.835 1.42 0.905 2.3 1.14 < 0.498 < 0.495 < 0.498 0.548 0.829 0.639 0.85 < 0.493 0.898 0.5 0.866 0.851 PFDoA < 0.500 < 0.488 < 0.488 < 0.493 0.564 < 0.500 < 0.500 1.64 1.73 < 0.490 0.983 0.666 1.43 1.42 1.83 1.62 3.91 1.73 < 0.498 < 0.495 < 0.498 < 0.493 0.727 1.57 0.753 < 0.493 1.52 < 0.488 1.23 1.13 PFBS < 1.00 < 0.976 < 0.976 < 0.985 < 0.980 < 1.00 < 1.00 < 0.995 < 0.976 < 0.980 < 1.00 < 0.966 < 1.00 < 0.976 < 0.980 < 0.976 < 0.990 < 0.976 < 0.995 < 0.990 < 0.995 < 0.985 < 0.980 < 0.995 < 1.00 < 0.985 < 1.00 < 0.976 < 0.976 < 0.995 PFHxS < 1.00 < 0.976 < 0.976 < 0.985 < 0.980 < 1.00 < 1.00 < 0.995 < 0.976 < 0.980 < 1.00 < 0.966 < 1.00 < 0.976 < 0.980 < 0.976 < 0.990 < 0.976 < 0.995 < 0.990 < 0.995 < 0.985 < 0.980 < 0.995 < 1.00 < 0.985 < 1.00 < 0.976 < 0.976 < 0.995 PFOS < 1.00 1.02 4.01 7.67 6.24 3.84 8.38 55 40.2 2.37 16.4 21.4 12.5 28.2 30.2 30.1 50.5 22.3 18.4 23.8 9.46 44.4 36.6 3.99 63.3 24.3 46.4 46.1 56.9 47.4 PFOSA < 0.600 < 0.585 < 0.585 < 0.591 < 0.588 < 0.600 < 0.600 < 0.597 < 0.585 < 0.588 < 0.600 < 0.580 < 0.600 < 0.585 < 0.588 < 0.585 0.626 < 0.585 < 0.597 < 0.594 < 0.597 < 0.591 < 0.588 < 0.597 < 0.600 < 0.591 < 0.600 < 0.585 < 0.585 < 0.597 QA Review Date 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 Qualified Analytes PFOS, PFDA Perfluorochemicals in Mississippi River Pool 2 May 2013 24 Minnesota Pollution Control Agency CLIENT ID 123106 123107 123108 123109 123110 123111 123112 123113 123114 123115 123116 123117 123118 123119 123120 123121 123122 123123 123124 123125 123126 123127 123128 123129 123130 123131 123132 123133 123134 123135 123136 WORK GROUP WG40902 WG40902 WG40902 WG40902 WG40902 WG40902 WG40902 WG40902 WG40903 WG40903 WG40903 WG40903 WG40903 WG40903 WG40903 WG40903 WG40903 WG40903 WG40898 WG40898 WG40898 WG40898 WG40898 WG40898 WG40898 WG40898 WG40898 WG40898 WG40911 WG40911 WG40911 Sample (g - wet) 2.03 2.02 2.03 2.02 2.02 1.99 2.02 1.99 2.03 1.98 1.99 2.00 1.98 2.00 1.99 2.06 2.02 2.01 2.06 1.99 1.99 2.01 1.99 2.04 2.05 2.03 2.02 2.06 1.98 2.06 2.06 PFBA < 0.493 < 0.495 < 0.493 < 0.495 < 0.495 < 0.503 < 0.495 < 0.503 < 0.493 < 0.505 < 0.503 < 0.500 < 0.505 < 0.500 < 0.503 < 0.485 < 0.495 < 0.498 < 0.485 < 0.503 < 0.503 < 0.498 < 0.503 < 0.490 < 0.488 < 0.493 < 0.495 < 0.485 < 0.505 < 0.485 < 0.485 PFPeA < 0.493 < 0.495 < 0.493 < 0.495 < 0.495 < 0.503 < 0.495 < 0.503 < 0.493 < 0.505 < 0.503 < 0.500 < 0.505 < 0.500 < 0.503 < 0.485 < 0.495 < 0.498 < 0.485 < 0.503 < 0.503 < 0.498 < 0.503 < 0.490 < 0.488 < 0.493 < 0.495 < 0.485 < 0.505 < 0.485 < 0.485 PFHxA < 0.493 < 0.495 < 0.493 < 0.495 < 0.495 < 0.503 < 0.495 < 0.503 < 0.493 < 0.505 < 0.503 < 0.500 < 0.505 < 0.500 < 0.503 < 0.485 < 0.495 < 0.498 < 0.485 < 0.503 < 0.503 < 0.498 < 0.503 < 0.490 < 0.488 < 0.493 < 0.495 < 0.485 < 0.505 < 0.485 < 0.485 PFHpA < 0.493 < 0.495 < 0.493 < 0.495 < 0.495 < 0.503 < 0.495 < 0.503 < 0.493 < 0.505 < 0.503 < 0.500 < 0.505 < 0.500 < 0.503 < 0.485 < 0.495 < 0.498 < 0.485 < 0.503 < 0.503 < 0.498 < 0.503 < 0.490 < 0.488 < 0.493 < 0.495 < 0.485 < 0.505 < 0.485 < 0.485 PFOA < 0.493 < 0.495 < 0.493 < 0.495 < 0.495 < 0.503 < 0.495 < 0.503 < 0.493 < 0.505 < 0.503 < 0.500 < 0.505 < 0.500 < 0.503 < 0.485 < 0.495 < 0.498 < 0.485 < 0.503 < 0.503 < 0.498 < 0.503 < 0.490 < 0.488 < 0.493 < 0.495 < 0.485 < 0.505 < 0.485 < 0.485 PFNA < 0.493 < 0.495 < 0.493 < 0.495 < 0.495 < 0.503 < 0.495 < 0.503 < 0.493 < 0.505 < 0.503 < 0.500 < 0.505 < 0.500 < 0.503 < 0.485 < 0.495 < 0.498 < 0.485 < 0.503 < 0.503 < 0.498 < 0.503 < 0.490 < 0.488 < 0.493 < 0.495 1.0 < 0.505 < 0.485 0.668 PFDA 1.25 1.8 1.11 0.957 0.903 0.849 2.67 1.25 0.892 1.84 1.55 1.84 1.08 0.78 2.0 0.982 0.707 1.36 1.2 0.773 < 0.503 0.991 < 0.503 < 0.490 1.89 2.53 1.54 2.82 < 0.505 0.654 3.62 PFUnA 0.77 0.959 < 0.493 < 0.495 < 0.495 < 0.503 1.61 0.558 < 0.493 0.905 0.553 0.676 0.81 < 0.500 0.934 0.553 < 0.495 0.977 < 0.485 0.511 < 0.503 < 0.498 < 0.503 < 0.490 0.789 1.26 0.718 1.36 < 0.505 < 0.485 1.54 PFDoA 1.01 0.621 < 0.493 < 0.495 < 0.495 < 0.503 0.68 0.673 0.572 0.952 < 0.503 0.528 0.725 0.617 1.14 0.591 < 0.495 < 0.498 < 0.485 0.584 < 0.503 < 0.498 < 0.503 < 0.490 1.02 1.05 0.837 1.06 < 0.505 < 0.485 1.22 PFBS < 0.985 < 0.990 < 0.985 < 0.990 < 0.990 < 1.01 < 0.990 < 1.01 < 0.985 < 1.01 < 1.01 < 1.00 < 1.01 < 1.00 < 1.01 < 0.971 < 0.990 < 0.995 < 0.971 < 1.01 < 1.01 < 0.995 < 1.01 < 0.980 < 0.976 < 0.985 < 0.990 < 0.971 < 1.01 < 0.971 < 0.971 PFHxS < 0.985 < 0.990 < 0.985 < 0.990 < 0.990 < 1.01 < 0.990 < 1.01 < 0.985 < 1.01 < 1.01 < 1.00 < 1.01 < 1.00 < 1.01 < 0.971 < 0.990 < 0.995 < 0.971 < 1.01 < 1.01 < 0.995 < 1.01 < 0.980 < 0.976 < 0.985 < 0.990 < 0.971 < 1.01 < 0.971 < 0.971 PFOS 56.8 8.51 20.7 7.46 34.9 21.8 6.8 9.29 20.6 53.1 15.5 12.2 41.8 10.9 96.8 24.2 22.3 41.8 40 34.6 3.71 37 11.3 1.56 36.2 42.5 30 38.7 2.06 23.3 80.3 PFOSA < 0.591 < 0.594 < 0.591 < 0.594 < 0.594 < 0.603 < 0.594 < 0.603 < 0.591 < 0.606 < 0.603 < 0.600 < 0.606 < 0.600 < 0.603 < 0.583 < 0.594 < 0.597 < 0.485 < 0.503 < 0.503 < 0.498 < 0.503 < 0.490 < 0.488 < 0.493 < 0.495 0.698 < 0.606 < 0.583 < 0.583 QA Review Date 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 10/4/2012 10/4/2012 10/4/2012 Qualified Analytes PFDoA PFOSA Perfluorochemicals in Mississippi River Pool 2 May 2013 25 Minnesota Pollution Control Agency CLIENT ID 123137 123138 123139 123140 123141 123142 123143 123144 123145 123146 123147 123148 123149 123150 123151 123152 123153 123154 123155 123156 123157 123158 123159 123160 123161 123162 123163 123164 123165 123166 123167 WORK GROUP WG40911 WG40911 WG40911 WG40911 WG40911 WG40911 WG40911 WG40911 WG40911 WG40911 WG40898 WG40898 WG40898 WG40898 WG40898 WG40898 WG40898 WG41155 WG40898 WG40898 WG40903 WG40903 WG40903 WG40903 WG40903 WG40903 WG40903 WG40903 WG40903 WG40904 WG40904 Sample (g - wet) 2.04 2.09 2.06 1.98 2.00 1.99 2.02 1.96 2.01 2.06 2.01 2.02 2.05 2.05 1.99 2.00 2.01 1.97 2.05 2.01 2.03 2.15 2.03 2.00 2.08 2.01 2.11 2.05 1.98 1.99 2.01 PFBA < 0.490 < 0.478 < 0.485 < 0.505 < 0.500 < 0.503 < 0.495 < 0.510 < 0.498 < 0.485 < 0.498 < 0.495 < 0.488 < 0.488 < 0.503 < 0.500 < 0.498 < 2.54 < 0.488 < 0.498 < 0.493 < 0.465 < 0.493 < 0.500 < 0.481 < 0.498 < 0.474 < 0.488 < 0.505 < 0.503 < 0.498 PFPeA < 0.490 < 0.478 < 0.485 < 0.505 < 0.500 < 0.503 < 0.495 < 0.510 < 0.498 < 0.485 < 0.498 < 0.495 < 0.488 < 0.488 < 0.503 < 0.500 < 0.498 < 2.54 < 0.488 < 0.498 < 0.493 < 0.465 < 0.493 < 0.500 < 0.481 < 0.498 < 0.474 < 0.488 < 0.505 < 0.503 < 0.498 PFHxA < 0.490 < 0.478 < 0.485 < 0.505 < 0.500 < 0.503 < 0.495 < 0.510 < 0.498 < 0.485 < 0.498 < 0.495 < 0.488 < 0.488 < 0.503 < 0.500 < 0.498 < 2.54 < 0.488 < 0.498 < 0.493 < 0.465 < 0.493 < 0.500 < 0.481 < 0.498 < 0.474 < 0.488 < 0.505 < 0.503 < 0.498 PFHpA < 0.490 < 0.478 < 0.485 < 0.505 < 0.500 < 0.503 < 0.495 < 0.510 < 0.498 < 0.485 < 0.498 < 0.495 < 0.488 < 0.488 < 0.503 < 0.500 < 0.498 < 2.54 < 0.488 < 0.498 < 0.493 < 0.465 < 0.493 < 0.500 < 0.481 < 0.498 < 0.474 < 0.488 < 0.505 < 0.503 < 0.498 PFOA < 0.490 < 0.478 < 0.485 < 0.505 < 0.500 < 0.503 < 0.495 < 0.510 < 0.498 < 0.485 < 0.498 < 0.495 < 0.488 < 0.488 < 0.503 < 0.500 < 0.498 < 2.54 < 0.488 < 0.498 < 0.493 < 0.465 < 0.493 < 0.500 < 0.481 < 0.498 < 0.474 < 0.488 < 0.505 < 0.503 < 0.498 PFNA 0.865 < 0.478 0.608 < 0.505 < 0.500 < 0.503 < 0.495 < 0.510 < 0.498 1.2 0.792 0.568 0.57 0.701 0.865 0.717 < 0.498 < 2.54 < 0.488 0.707 1.06 0.787 1.03 0.755 < 0.481 < 0.498 < 0.474 < 0.488 < 0.505 < 0.503 < 0.498 PFDA 3.01 1.76 2.6 < 0.505 1.1 0.548 < 0.495 < 0.510 0.506 5.44 4.12 3.28 2.37 2.84 4.0 4.23 2.53 3.27 2.15 3.43 2.68 2.65 3.51 3.24 1.23 1.55 0.725 0.935 0.599 0.785 < 0.498 PFUnA 1.6 1.09 1.38 < 0.505 0.629 < 0.503 < 0.495 < 0.510 < 0.498 2.69 2.06 1.56 1.44 1.54 1.91 2.0 1.12 < 2.54 0.964 2.13 0.993 1.21 1.66 1.49 0.498 0.878 < 0.474 < 0.488 < 0.505 < 0.503 < 0.498 PFDoA 1.84 1.47 1.45 < 0.505 < 0.500 < 0.503 < 0.495 < 0.510 < 0.498 2.49 1.68 1.25 1.4 1.21 1.74 2.16 1.08 < 2.54 0.725 1.86 1.21 1.19 1.43 1.44 0.636 1.45 1.26 0.997 < 0.505 0.514 < 0.498 PFBS < 0.980 < 0.957 < 0.971 < 1.01 < 1.00 < 1.01 < 0.990 < 1.02 < 0.995 < 0.971 < 0.995 < 0.990 < 0.976 < 0.976 < 1.01 < 1.00 < 0.995 < 5.08 < 0.976 < 0.995 < 0.985 < 0.930 < 0.985 < 1.00 < 0.962 < 0.995 < 0.948 < 0.976 < 1.01 < 1.01 < 0.995 PFHxS < 0.980 < 0.957 < 0.971 < 1.01 < 1.00 < 1.01 < 0.990 < 1.02 < 0.995 < 0.971 < 0.995 < 0.990 < 0.976 < 0.976 < 1.01 < 1.00 < 0.995 < 5.08 < 0.976 < 0.995 < 0.985 < 0.930 < 0.985 < 1.00 < 0.962 < 0.995 < 0.948 < 0.976 < 1.01 < 1.01 < 0.995 PFOS 55.7 29.6 35.4 3.12 17.5 28.4 2.38 2.82 8.14 66.8 44.4 41.3 35 44.5 58.3 77.5 40.1 47.9 39.5 38.8 35.5 54 50.3 32.8 18.5 18.6 9.22 9.53 13.9 38.4 13.8 PFOSA 3.25 < 0.574 < 0.583 < 0.606 < 0.600 < 0.603 < 0.594 < 0.612 < 0.597 0.718 0.669 < 0.495 < 0.488 0.793 1.22 J 0.570 1.18 < 2.54 < 0.488 J 0.555 < 0.591 0.866 0.653 < 0.600 < 0.577 < 0.597 < 0.569 < 0.585 < 0.606 < 0.603 < 0.597 QA Review Date 10/4/2012 10/4/2012 10/4/2012 10/4/2012 10/4/2012 10/4/2012 10/4/2012 10/4/2012 10/4/2012 10/4/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 9/26/2012 10/15/2012 9/26/2012 9/26/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 Qualified Analytes PFOSA PFOSA PFOSA PFOSA PFDoA PFDoA Perfluorochemicals in Mississippi River Pool 2 May 2013 26 Minnesota Pollution Control Agency CLIENT ID 123168 123169 123170 123171 123172 123173 123174 123175 123176 123177 123178 123179 123180 123181 123182 123183 123184 123185 123186 123187 123188 123189 123190 123191 123192 123193 123194 123195 123196 123197 123198 WORK GROUP WG40904 WG40904 WG40904 WG40904 WG40904 WG40904 WG40904 WG40904 WG40904 WG40904 WG40904 WG41230 WG41230 WG41230 WG41230 WG41230 WG41230 WG41230 WG41230 WG40910 WG40910 WG40910 WG40910 WG40910 WG40910 WG40910 WG40910 WG40910 WG40910 WG40910 WG40910 Sample (g - wet) 2.03 2.00 2.01 2.01 1.99 2.00 2.00 2.02 1.99 2.02 2.00 2.02 2.03 2.05 2.03 1.99 1.98 2.00 1.98 2.12 2.03 2.01 1.99 1.99 2.01 2.28 2.22 2.07 2.14 2.22 2.17 PFBA < 0.493 < 0.500 < 0.498 < 0.498 < 0.503 < 0.500 0.689 < 0.495 < 0.503 < 0.495 < 0.500 < 0.495 < 0.493 < 0.488 < 0.493 < 0.503 < 0.505 < 0.500 < 0.505 < 0.472 < 0.493 < 0.498 < 0.503 < 0.503 < 0.498 < 0.439 < 0.450 < 0.483 < 0.467 < 0.450 < 0.461 PFPeA < 0.493 < 0.500 < 0.498 < 0.498 < 0.503 < 0.500 < 0.500 < 0.495 < 0.503 < 0.495 < 0.500 < 0.495 < 0.493 < 0.488 < 0.493 < 0.503 < 0.505 < 0.500 < 0.505 < 0.472 < 0.493 < 0.498 < 0.503 < 0.503 < 0.498 < 0.439 < 0.450 < 0.483 < 0.467 < 0.450 < 0.461 PFHxA < 0.493 < 0.500 < 0.498 < 0.498 < 0.503 < 0.500 < 0.500 < 0.495 < 0.503 < 0.495 < 0.500 < 0.495 < 0.493 < 0.488 < 0.493 < 0.503 < 0.505 < 0.500 < 0.505 < 0.472 < 0.493 < 0.498 < 0.503 < 0.503 < 0.498 < 0.439 < 0.450 < 0.483 < 0.467 < 0.450 < 0.461 PFHpA < 0.493 < 0.500 < 0.498 < 0.498 < 0.503 < 0.500 < 0.500 < 0.495 < 0.503 < 0.495 < 0.500 < 0.495 < 0.493 < 0.488 < 0.493 < 0.503 < 0.505 < 0.500 < 0.505 < 0.472 < 0.493 < 0.498 < 0.503 < 0.503 < 0.498 < 0.439 < 0.450 < 0.483 < 0.467 < 0.450 < 0.461 PFOA < 0.493 < 0.500 < 0.498 < 0.498 < 0.503 < 0.500 < 0.500 < 0.495 < 0.503 < 0.495 < 0.500 < 0.495 < 0.493 < 0.488 < 0.493 < 0.503 < 0.505 < 0.500 < 0.505 < 0.472 < 0.493 < 0.498 < 0.503 < 0.503 < 0.498 < 0.439 < 0.450 < 0.483 < 0.467 < 0.450 < 0.461 PFNA < 0.493 < 0.500 < 0.498 < 0.498 < 0.503 < 0.500 < 0.500 < 0.495 1.64 < 0.495 < 0.500 < 0.495 < 0.493 < 0.488 < 0.493 < 0.503 < 0.505 < 0.500 1.38 0.777 0.694 1.27 < 0.503 < 0.503 < 0.498 < 0.439 < 0.450 < 0.483 < 0.467 < 0.450 < 0.461 PFDA < 0.493 < 0.500 1.3 < 0.498 < 0.503 < 0.500 < 0.500 < 0.495 1.79 4.37 0.925 0.692 1.68 1.27 1.46 1.53 1.74 2.16 4.55 1.43 2.67 2.91 1.53 1.74 1.25 1.59 0.807 1.33 1.35 1.36 1.45 PFUnA < 0.493 < 0.500 0.76 < 0.498 < 0.503 < 0.500 < 0.500 < 0.495 0.688 4.4 < 0.500 0.567 1.79 0.927 0.647 1.4 1.6 1.11 2.2 0.707 1.47 1.49 1.82 2.15 1.28 1.08 1.05 1.5 1.42 2.17 1.15 PFDoA < 0.493 < 0.500 1.75 < 0.498 < 0.503 < 0.500 < 0.500 < 0.495 < 0.503 5.19 < 0.500 0.569 1.73 1.01 0.914 1.66 1.52 0.999 2.09 0.602 1.18 1.26 2.07 2.43 1.63 1.49 1.38 1.86 1.45 5.22 1.04 PFBS < 0.985 < 1.00 < 0.995 < 0.995 < 1.01 < 1.00 < 1.00 < 0.990 < 1.01 < 0.990 < 1.00 < 0.990 < 0.985 < 0.976 < 0.985 < 1.01 < 1.01 < 1.00 < 1.01 < 0.943 < 0.985 < 0.995 < 1.01 < 1.01 < 0.995 < 0.877 < 0.901 < 0.966 < 0.935 < 0.901 < 0.922 PFHxS < 0.985 < 1.00 < 0.995 < 0.995 < 1.01 < 1.00 < 1.00 < 0.990 < 1.01 < 0.990 < 1.00 < 0.990 < 0.985 < 0.976 < 0.985 < 1.01 < 1.01 < 1.00 < 1.01 < 0.943 < 0.985 < 0.995 < 1.01 < 1.01 < 0.995 < 0.877 < 0.901 < 0.966 < 0.935 < 0.901 < 0.922 PFOS 15.1 9.95 113 6.47 17.5 24 7.84 9.87 34.2 17.1 13.4 19 6.01 23.3 104 8.17 8.17 37.5 153 27.1 61.9 41.3 47.1 67.5 66.4 74 8.11 45.7 26.6 103 30.8 PFOSA < 0.591 < 0.600 0.774 < 0.597 < 0.603 < 0.600 < 0.600 < 0.594 < 0.603 < 0.594 < 0.600 < 0.594 < 0.591 < 0.585 1.92 < 0.603 < 0.606 < 0.600 2.28 0.675 0.943 0.944 0.798 2.52 < 0.597 1.14 1.02 1.23 0.811 2.89 < 0.553 QA Review Date 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 10/5/2012 10/5/2012 10/5/2012 10/5/2012 10/5/2012 10/5/2012 10/5/2012 10/5/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 Qualified Analytes PFDoA, PFOSA PFOS Perfluorochemicals in Mississippi River Pool 2 May 2013 27 Minnesota Pollution Control Agency CLIENT ID 123199 123200 123201 123202 123203 123204 123205 123206 123207 WORK GROUP WG40910 WG40910 WG40910 WG40910 WG40910 WG40910 WG40907 WG40907 WG40907 Sample (g - wet) 2.23 2.13 2.01 2.31 2.08 2.04 1.99 2.01 2.01 PFBA < 0.448 1.18 < 0.498 < 0.433 < 0.481 < 0.490 24.8 9.08 11.6 PFPeA < 0.448 < 0.469 < 0.498 < 0.433 < 0.481 < 0.490 0.732 < 1.49 0.544 PFHxA < 0.448 < 0.469 < 0.498 < 0.433 < 0.481 < 0.490 < 0.503 < 1.49 < 0.498 PFHpA < 0.448 < 0.469 < 0.498 < 0.433 < 0.481 < 0.490 < 0.503 < 1.49 < 0.498 PFOA < 0.448 0.887 < 0.498 < 0.433 < 0.481 < 0.490 8.89 1.32 1.29 PFNA < 0.448 < 0.469 < 0.498 < 0.433 < 0.481 < 0.490 0.505 < 0.498 < 0.498 PFDA 1.08 1.93 2.49 1.61 1.04 0.689 11.5 < 0.498 < 0.498 PFUnA 0.985 1.04 3.74 1.11 0.823 0.534 3.41 < 0.498 < 0.498 PFDoA 0.82 1.17 4.61 1.18 0.751 0.687 4.6 < 0.498 0.847 PFBS < 0.897 1.3 < 0.995 < 0.866 < 0.962 < 0.980 < 1.01 < 0.995 < 0.995 PFHxS < 0.897 < 0.939 < 0.995 < 0.866 < 0.962 < 0.980 13.2 < 0.995 1.52 PFOS 44.8 28.4 1020 47.4 46 11 6160 25.4 28 123208 WG40907 2.02 5.96 < 0.495 < 0.495 < 0.495 0.67 < 0.495 0.788 < 0.495 0.673 < 0.990 < 0.990 132 123209 WG40907 1.97 2.44 < 0.508 < 0.508 < 0.508 1.61 < 0.508 6.16 4.89 26.3 < 1.02 1.49 440 123210 WG40907 2.00 3.96 < 0.500 < 0.500 < 0.500 1.13 < 0.500 8.62 6.79 30.3 < 1.00 < 1.00 661 123211 WG40907 2.02 < 0.495 < 0.495 < 0.495 < 0.495 < 0.495 < 0.495 7.78 6.14 18.1 < 0.990 < 0.990 718 123212 WG40907 1.98 0.875 < 0.505 < 0.505 < 0.505 < 0.505 < 0.505 7.02 3.01 8.85 < 1.01 < 1.01 1580 123213 WG40907 2.02 0.845 < 0.495 < 0.495 < 0.495 < 0.495 < 0.495 9.64 4.59 9.62 < 0.990 < 0.990 1560 123214 WG40907 2.00 1.37 < 0.500 < 0.500 < 0.500 < 0.500 < 0.500 2.05 1.75 8.59 < 1.00 < 1.00 192 123215 WG40908 1.96 0.958 < 0.510 < 0.510 < 0.510 < 0.510 < 0.510 0.729 < 0.510 < 0.510 < 1.02 < 1.02 8.24 123216 WG40908 1.99 < 0.503 < 0.503 < 0.503 < 0.503 < 0.503 < 0.503 0.654 < 0.503 < 0.503 < 1.01 < 1.01 5.99 123217 WG40908 2.14 < 0.467 < 0.467 < 0.467 < 0.467 < 0.467 0.539 1.27 0.754 0.939 < 0.935 < 0.935 10.8 123218 WG40908 2.08 < 0.481 < 0.481 < 0.481 < 0.481 < 0.481 < 0.481 1.46 1.14 1.27 < 0.962 < 0.962 19.6 123219 WG40908 2.01 < 0.498 < 0.498 < 0.498 < 0.498 < 0.498 < 0.498 2.66 2.8 3.65 < 0.995 < 0.995 55.4 123220 WG40908 2.00 < 0.500 < 0.500 < 0.500 < 0.500 < 0.500 < 0.500 1.71 1.13 2.02 < 1.00 < 1.00 112 123221 WG40908 2.00 < 0.500 < 0.500 < 0.500 < 0.500 < 0.500 < 0.500 1.59 0.67 0.656 < 1.00 < 1.00 29.1 123222 WG40908 1.99 < 0.503 < 0.503 < 0.503 < 0.503 < 0.503 < 0.503 1.22 1.53 2.0 < 1.01 < 1.01 14.6 123223 WG40908 1.99 < 0.503 < 0.503 < 0.503 < 0.503 < 0.503 < 0.503 1.49 1.07 1.36 < 1.01 < 1.01 27.9 123224 WG40908 2.01 < 0.498 < 0.498 < 0.498 < 0.498 < 0.498 1.92 4.28 2.0 1.46 < 0.995 < 0.995 87 123225 WG40908 2.01 < 0.498 < 0.498 < 0.498 < 0.498 < 0.498 0.983 2.63 0.854 0.89 < 0.995 < 0.995 53.2 123226 WG40908 2.00 < 0.500 < 0.500 < 0.500 < 0.500 < 0.500 1.06 3.0 1.42 1.36 < 1.00 < 1.00 45.5 123227 WG40908 2.01 < 0.498 < 0.498 < 0.498 < 0.498 < 0.498 1.26 2.32 0.876 0.745 < 0.995 < 0.995 44.5 123228 WG40908 2.09 < 0.478 < 0.478 < 0.478 < 0.478 < 0.478 1.2 2.11 0.973 0.743 < 0.957 < 0.957 31.1 PFOSA < 0.538 < 0.563 6.21 < 0.519 < 0.577 < 0.588 19.5 4.53 8.74 4.61 34.7 51.6 17.1 31.3 23.8 46.9 < 0.612 < 0.603 < 0.561 0.713 2.14 3.45 0.603 < 0.603 0.631 1.17 1.1 1.26 1.6 0.834 QA Review Date 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 10/4/2012 10/4/2012 10/4/2012 10/4/2012 10/4/2012 10/4/2012 10/4/2012 10/4/2012 10/4/2012 10/4/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 Qualified Analytes PFDoA PFPeA, PFDoA, PFOSA, PFOS PFDoA PFDA Perfluorochemicals in Mississippi River Pool 2 May 2013 28 Minnesota Pollution Control Agency CLIENT ID 123229 123230 123231 123232 123233 123237 123238 123239 123240 123241 123242 123243 123244 123245 123246 123247 123248 123249 123250 123251 123252 123253 123254 123255 123256 123257 123258 123259 123260 123261 123262 WORK GROUP WG40908 WG40908 WG40908 WG40908 WG40908 WG40909 WG40904 WG40904 WG40904 WG40904 WG40904 WG40904 WG40905 WG40905 WG40905 WG40905 WG40905 WG40905 WG40905 WG40905 WG40905 WG40905 WG40905 WG40905 WG40905 WG40905 WG40905 WG40905 WG40905 WG40905 WG40905 Sample (g - wet) 2.09 1.99 2.03 2.00 2.01 2.05 1.99 2.00 2.03 1.99 2.01 2.02 1.98 1.99 2.00 2.01 2.08 2.04 1.98 1.97 2.01 1.97 2.00 2.02 1.99 2.01 1.98 2.07 2.01 2.06 2.04 PFBA < 0.478 < 0.503 < 0.493 < 0.500 < 0.498 < 0.488 < 0.503 < 0.500 < 0.493 < 0.503 < 0.498 < 0.495 < 0.505 1.77 < 0.500 < 0.498 < 0.481 < 0.490 < 0.505 < 0.508 < 0.498 1.14 < 0.500 < 0.495 < 0.503 < 0.498 < 0.505 < 0.483 < 0.498 < 0.485 < 0.490 PFPeA < 0.478 < 0.503 < 0.493 < 0.500 < 0.498 < 0.488 < 0.503 < 0.500 < 0.493 < 0.503 < 0.498 < 0.495 < 0.505 < 0.503 < 0.500 < 0.498 < 0.481 < 0.490 < 0.505 < 0.508 < 0.498 < 0.508 < 0.500 < 0.495 < 0.503 < 0.498 < 0.505 < 0.483 < 0.498 < 0.485 < 0.490 PFHxA < 0.478 < 0.503 < 0.493 < 0.500 < 0.498 < 0.488 < 0.503 < 0.500 < 0.493 < 0.503 < 0.498 < 0.495 < 0.505 < 0.503 < 0.500 < 0.498 < 0.481 < 0.490 < 0.505 < 0.508 < 0.498 < 0.508 < 0.500 < 0.495 < 0.503 < 0.498 < 0.505 < 0.483 < 0.498 < 0.485 < 0.490 PFHpA < 0.478 < 0.503 < 0.493 < 0.500 < 0.498 < 0.488 < 0.503 < 0.500 < 0.493 < 0.503 < 0.498 < 0.495 < 0.505 < 0.503 < 0.500 < 0.498 < 0.481 < 0.490 < 0.505 < 0.508 < 0.498 < 0.508 < 0.500 < 0.495 < 0.503 < 0.498 < 0.505 < 0.483 < 0.498 < 0.485 < 0.490 PFOA < 0.478 < 0.503 < 0.493 < 0.500 < 0.498 < 0.488 < 0.503 < 0.500 < 0.493 < 0.503 < 0.498 < 0.495 < 0.505 < 0.503 < 0.500 < 0.498 < 0.481 < 0.490 < 0.505 < 0.508 < 0.498 < 0.508 < 0.500 < 0.495 < 0.503 < 0.498 < 0.505 < 0.483 < 0.498 < 0.485 < 0.490 PFNA 0.684 < 0.503 < 0.493 < 0.500 < 0.498 < 0.488 0.734 1.15 < 0.493 < 0.503 < 0.498 < 0.495 < 0.505 < 0.503 < 0.500 < 0.498 < 0.481 < 0.490 < 0.505 < 0.508 < 0.498 < 0.508 < 0.500 < 0.495 < 0.503 < 0.498 < 0.505 < 0.483 < 0.498 < 0.485 < 0.490 PFDA 8.52 1.54 1.07 0.72 1.33 1.98 2.03 2.58 0.546 0.811 0.931 1.75 1.08 0.943 0.64 0.629 0.976 0.493 1.34 0.596 0.956 0.705 < 0.500 1.01 0.535 < 0.498 < 0.505 < 0.483 < 0.498 1.99 1.02 PFUnA 3.48 0.626 0.626 < 0.500 1.08 1.33 0.872 1.03 < 0.493 0.562 0.625 1.02 0.573 < 0.503 0.561 0.779 0.94 < 0.490 0.722 < 0.508 0.556 0.627 < 0.500 0.835 < 0.503 < 0.498 < 0.505 < 0.483 < 0.498 1.51 < 0.490 PFDoA 2.68 0.595 < 0.493 < 0.500 0.879 1.28 1.09 0.932 < 0.493 < 0.503 0.963 0.97 0.615 < 0.503 < 0.500 0.71 0.934 < 0.490 0.629 < 0.508 0.593 0.52 < 0.500 1.87 < 0.503 < 0.498 < 0.505 < 0.483 < 0.498 3.05 < 0.490 PFBS < 0.957 < 1.01 < 0.985 < 1.00 < 0.995 < 0.976 < 1.01 < 1.00 < 0.985 < 1.01 < 0.995 < 0.990 < 1.01 < 1.01 < 1.00 < 0.995 < 0.962 < 0.980 < 1.01 < 1.02 < 0.995 < 1.02 < 1.00 < 0.990 < 1.01 < 0.995 < 1.01 < 0.966 < 0.995 < 0.971 < 0.980 PFHxS < 0.957 < 1.01 < 0.985 < 1.00 < 0.995 < 0.976 < 1.01 < 1.00 < 0.985 < 1.01 < 0.995 < 0.990 < 1.01 < 1.01 < 1.00 < 0.995 < 0.962 < 0.980 < 1.01 < 1.02 < 0.995 < 1.02 < 1.00 < 0.990 < 1.01 < 0.995 < 1.01 < 0.966 < 0.995 < 0.971 < 0.980 PFOS 82.6 18.9 17.1 13.4 25.4 45.2 41.2 52.2 12.1 26.5 40.4 53 44.9 34.5 24.5 36.2 60.4 19.9 37.9 26.1 31.1 25.2 12.8 40.5 8.13 < 0.995 2.26 1.52 < 0.995 12.9 18.3 PFOSA < 0.574 < 0.603 < 0.591 < 0.600 < 0.597 0.982 < 0.603 0.801 < 0.591 < 0.603 < 0.597 < 0.594 < 0.606 < 0.603 < 0.600 < 0.597 < 0.577 < 0.588 < 0.606 < 0.609 < 0.597 < 0.609 < 0.600 < 0.594 < 0.603 < 0.597 < 0.606 < 0.580 < 0.597 < 0.583 < 0.588 QA Review Date 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 Qualified Analytes PFBA PFBA, PFDA PFDoA Perfluorochemicals in Mississippi River Pool 2 May 2013 29 Minnesota Pollution Control Agency CLIENT ID 123263 123264 123265 123266 123267 123268 123269 123270 123271 123272 123273 123274 123275 123276 123277 123278 123279 123280 123281 123282 123283 123284 123285 123286 123287 123288 123289 123290 123291 123292 123293 WORK GROUP WG40906 WG40906 WG40906 WG40906 WG40906 WG40906 WG40906 WG40906 WG40906 WG40906 WG40906 WG40906 WG40906 WG40906 WG40906 WG40906 WG40906 WG40906 WG40906 WG40907 WG40907 WG40907 WG40907 WG40907 WG40907 WG40907 WG40907 WG40907 WG40909 WG40909 WG40909 Sample (g - wet) 1.97 1.99 1.97 1.98 2.03 2.03 2.02 1.99 2.02 1.97 1.98 1.97 1.98 2.00 2.00 2.02 1.97 1.99 2.03 2.05 1.97 2.02 1.97 2.01 1.97 1.99 2.00 1.97 2.04 1.99 2.05 PFBA < 0.508 < 0.503 < 0.508 < 0.505 < 0.493 < 0.493 < 0.495 < 0.503 < 0.495 < 0.508 < 0.505 < 0.508 < 0.505 < 0.500 < 0.500 < 0.495 < 0.508 < 0.503 < 0.493 < 0.488 < 0.508 < 0.495 < 0.508 < 0.498 < 0.508 < 0.503 < 0.500 < 0.508 < 0.490 < 0.503 < 0.488 PFPeA < 0.508 < 0.503 < 0.508 < 0.505 < 0.493 < 0.493 < 0.495 < 0.503 < 0.495 < 0.508 < 0.505 < 0.508 < 0.505 < 0.500 < 0.500 < 0.495 < 0.508 < 0.503 < 0.493 < 0.488 < 0.508 < 0.495 < 0.508 < 0.498 < 0.508 < 0.503 < 0.500 < 0.508 < 0.490 < 0.503 < 0.488 PFHxA < 0.508 < 0.503 < 0.508 < 0.505 < 0.493 < 0.493 < 0.495 < 0.503 < 0.495 < 0.508 < 0.505 < 0.508 < 0.505 < 0.500 < 0.500 < 0.495 < 0.508 < 0.503 < 0.493 < 0.488 < 0.508 < 0.495 < 0.508 < 0.498 < 0.508 < 0.503 < 0.500 < 0.508 < 0.490 < 0.503 < 0.488 PFHpA < 0.508 < 0.503 < 0.508 < 0.505 < 0.493 < 0.493 < 0.495 < 0.503 < 0.495 < 0.508 < 0.505 < 0.508 < 0.505 < 0.500 < 0.500 < 0.495 < 0.508 < 0.503 < 0.493 < 0.488 < 0.508 < 0.495 < 0.508 < 0.498 < 0.508 < 0.503 < 0.500 < 0.508 < 0.490 < 0.503 < 0.488 PFOA < 0.508 < 0.503 < 0.508 < 0.505 < 0.493 < 0.493 < 0.495 < 0.503 < 0.495 < 0.508 < 0.505 < 0.508 < 0.505 < 0.500 < 0.500 < 0.495 < 0.508 < 0.503 < 0.493 < 0.488 < 0.508 < 0.495 < 0.508 < 0.498 < 0.508 < 0.503 < 0.500 < 0.508 < 0.490 < 0.503 < 0.488 PFNA < 0.508 < 0.503 < 0.508 < 0.505 < 0.493 0.56 < 0.495 < 0.503 0.772 0.965 < 0.505 < 0.508 < 0.505 < 0.500 < 0.500 < 0.495 < 0.508 < 0.503 0.551 < 0.488 < 0.508 < 0.495 < 0.508 < 0.498 < 0.508 < 0.503 < 0.500 < 0.508 < 0.490 < 0.503 < 0.488 PFDA < 0.508 0.98 1.23 1.92 0.552 1.49 1.29 0.859 1.31 2.51 1.36 1.44 1.21 1.12 1.21 < 0.495 0.809 < 0.503 1.05 1.28 0.677 0.638 < 0.508 < 0.498 < 0.508 < 0.503 < 0.500 2.15 1.26 0.939 1.63 PFUnA < 0.508 < 0.503 0.733 0.743 < 0.493 0.555 0.707 0.609 0.63 1.09 0.624 0.664 0.509 0.625 1.2 < 0.495 < 0.508 < 0.503 0.549 0.546 < 0.508 < 0.495 < 0.508 < 0.498 < 0.508 < 0.503 0.548 1.58 0.859 0.51 1.25 PFDoA < 0.508 < 0.503 0.921 0.744 < 0.493 1.21 1.25 0.563 < 0.495 0.822 0.639 0.71 0.573 < 0.500 1.07 < 0.495 < 0.508 < 0.503 0.854 0.73 0.542 0.51 < 0.508 < 0.498 < 0.508 < 0.503 < 0.500 2.23 1.48 0.603 2.26 PFBS < 1.02 < 1.01 < 1.02 < 1.01 < 0.985 < 0.985 < 0.990 < 1.01 < 0.990 < 1.02 < 1.01 < 1.02 < 1.01 < 1.00 < 1.00 < 0.990 < 1.02 < 1.01 < 0.985 < 0.976 < 1.02 < 0.990 < 1.02 < 0.995 < 1.02 < 1.01 < 1.00 < 1.02 < 0.980 < 1.01 < 0.976 PFHxS < 1.02 < 1.01 < 1.02 < 1.01 < 0.985 < 0.985 < 0.990 < 1.01 < 0.990 < 1.02 < 1.01 < 1.02 < 1.01 < 1.00 < 1.00 < 0.990 < 1.02 < 1.01 < 0.985 < 0.976 < 1.02 < 0.990 < 1.02 < 0.995 < 1.02 < 1.01 < 1.00 < 1.02 < 0.980 < 1.01 < 0.976 PFOS 33.2 17.4 19.4 22.9 3.88 36.3 18.8 9.99 44.8 42.5 17.6 17.1 15.3 16.7 46.7 3.27 46.1 < 1.01 21.7 29.5 28.9 34.4 20.5 23.8 18.9 16.9 35.3 63.8 18 27.2 22.3 PFOSA < 0.609 < 0.603 < 0.609 < 0.606 < 0.591 < 0.591 < 0.594 < 0.603 < 0.594 < 0.609 < 0.606 < 0.609 < 0.606 < 0.600 < 0.600 < 0.594 < 0.609 < 0.603 < 0.591 < 0.585 1.01 1.36 < 0.609 < 0.597 < 0.609 < 0.603 < 0.600 < 0.609 < 0.588 < 0.603 < 0.585 QA Review Date 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 10/4/2012 10/4/2012 10/4/2012 10/4/2012 10/4/2012 10/4/2012 10/4/2012 10/4/2012 10/4/2012 9/27/2012 9/27/2012 9/27/2012 Qualified Analytes Perfluorochemicals in Mississippi River Pool 2 May 2013 30 Minnesota Pollution Control Agency CLIENT ID 123294 123295 123296 123297 123298 123299 123300 123301 123302 123303 123304 123305 123306 123307 123308 123309 123310 123311 123312 123313 123314 123315 123316 123317 123318 123319 123320 123321 123322 123323 123324 WORK GROUP WG40909 WG40909 WG40909 WG40909 WG40909 WG41230 WG41230 WG41230 WG41230 WG40910 WG40909 WG41230 WG41077 WG41077 WG41077 WG41077 WG41077 WG41077 WG41077 WG41077 WG41078 WG41078 WG41078 WG41078 WG41079 WG41079 WG41079 WG41079 WG41079 WG41079 WG41079 Sample (g - wet) 2.02 2.04 2.00 1.98 2.05 2.00 2.03 2.02 2.05 2.01 2.02 2.01 2.02 2.08 2.03 2.00 2.04 2.03 2.03 2.02 2.00 2.00 1.98 2.00 1.99 1.96 1.94 1.96 2.04 2.04 2.03 PFBA < 0.495 < 0.490 < 0.500 < 0.505 < 0.488 < 0.500 < 0.493 < 0.495 < 0.488 < 0.498 < 0.495 < 0.498 < 0.495 < 0.481 < 0.493 < 0.500 < 0.490 < 0.493 < 0.493 < 0.495 < 0.500 < 0.500 < 0.505 < 0.500 < 0.503 < 0.510 < 0.515 < 0.510 < 0.490 < 0.490 < 0.493 PFPeA < 0.495 < 0.490 < 0.500 < 0.505 < 0.488 < 0.500 < 0.493 < 0.495 < 0.488 < 0.498 < 0.495 < 0.498 < 0.495 < 0.481 < 0.493 < 0.500 < 0.490 < 0.493 < 0.493 < 0.495 < 0.500 < 0.500 < 0.505 < 0.500 < 0.503 < 0.510 < 0.515 < 0.510 < 0.490 < 0.490 < 0.493 PFHxA < 0.495 < 0.490 < 0.500 < 0.505 < 0.488 < 0.500 < 0.493 < 0.495 < 0.488 < 0.498 < 0.495 < 0.498 < 0.495 < 0.481 < 0.493 < 0.500 < 0.490 < 0.493 < 0.493 < 0.495 < 0.500 < 0.500 < 0.505 < 0.500 < 0.503 < 0.510 < 0.515 < 0.510 < 0.490 < 0.490 < 0.493 PFHpA < 0.495 < 0.490 < 0.500 < 0.505 < 0.488 < 0.500 < 0.493 < 0.495 < 0.488 < 0.498 < 0.495 < 0.498 < 0.495 < 0.481 < 0.493 < 0.500 < 0.490 < 0.493 < 0.493 < 0.495 < 0.500 < 0.500 < 0.505 < 0.500 < 0.503 < 0.510 < 0.515 < 0.510 < 0.490 < 0.490 < 0.493 PFOA < 0.495 < 0.490 < 0.500 < 0.505 < 0.488 < 0.500 < 0.493 < 0.495 < 0.488 < 0.498 < 0.495 < 0.498 < 0.495 < 0.481 0.5 < 0.500 < 0.490 < 0.493 < 0.493 < 0.495 < 0.500 < 0.500 < 0.505 < 0.500 < 0.503 < 0.510 < 0.515 < 0.510 < 0.490 < 0.490 < 0.493 PFNA < 0.495 < 0.490 < 0.500 < 0.505 < 0.488 < 0.500 < 0.493 < 0.495 < 0.488 < 0.498 < 0.495 < 0.498 < 0.495 < 0.481 < 0.493 < 0.500 < 0.490 < 0.493 < 0.493 < 0.495 < 0.500 < 0.500 < 0.505 < 0.500 < 0.503 0.612 < 0.515 0.774 < 0.490 < 0.490 < 0.493 PFDA 2.74 1.07 1.33 1.26 1.24 0.666 0.972 3.56 0.902 5.5 0.53 1.9 1.21 0.615 2.85 2.25 < 0.490 2.1 1.42 1.47 1.85 1.8 1.58 1.75 1.86 2.08 2.23 1.42 1.94 0.851 1.16 PFUnA 1.5 0.635 0.748 0.672 < 0.488 < 0.500 0.554 2.1 < 0.488 3.73 < 0.495 1.19 0.91 < 0.481 1.44 0.856 < 0.490 1.03 0.569 0.683 0.919 1.37 1.07 0.975 0.915 1.0 1.34 0.615 1.15 0.511 0.576 PFDoA 1.33 0.536 < 0.500 0.66 0.614 < 0.500 < 0.493 3.76 < 0.488 2.43 < 0.495 1.3 0.959 0.76 0.946 1.53 < 0.490 1.96 0.763 1.31 1.47 2.1 0.624 0.738 0.711 0.761 1.34 < 0.510 0.699 < 0.490 < 0.493 PFBS < 0.990 < 0.980 < 1.00 < 1.01 < 0.976 < 1.00 < 0.985 < 0.990 < 0.976 < 0.995 < 0.990 < 0.995 < 0.990 < 0.962 < 0.985 < 1.00 < 0.980 < 0.985 < 0.985 < 0.990 < 1.00 < 1.00 < 1.01 < 1.00 < 1.01 < 1.02 < 1.03 < 1.02 < 0.980 < 0.980 < 0.985 PFHxS < 0.990 < 0.980 < 1.00 < 1.01 < 0.976 < 1.00 < 0.985 < 0.990 < 0.976 < 0.995 < 0.990 < 0.995 < 0.990 < 0.962 < 0.985 < 1.00 < 0.980 < 0.985 < 0.985 < 0.990 < 1.00 < 1.00 < 1.01 < 1.00 < 1.01 < 1.02 < 1.03 < 1.02 < 0.980 < 0.980 < 0.985 PFOS 50.9 16.4 26.6 21.5 27.6 18.8 42.2 107 12.1 106 4.62 43.2 35.9 3.85 68.1 108 6.97 61.6 37.7 87.5 50.7 13.4 38.6 32.8 40.7 42.9 41.9 33.5 26.4 15.8 15.6 PFOSA < 0.594 < 0.588 < 0.600 < 0.606 0.97 < 0.600 < 0.591 0.754 < 0.585 < 0.597 < 0.594 < 0.597 < 0.594 < 0.577 0.769 0.848 < 0.588 < 0.591 < 0.591 < 0.594 < 0.600 < 0.600 1.16 1.41 1.63 1.46 1.46 1.85 0.728 < 0.588 < 0.591 QA Review Date 9/27/2012 9/27/2012 9/27/2012 9/27/2012 9/27/2012 10/5/2012 10/5/2012 10/5/2012 10/5/2012 9/27/2012 9/27/2012 10/5/2012 10/11/2012 10/11/2012 10/11/2012 10/11/2012 10/11/2012 10/11/2012 10/11/2012 10/11/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 Qualified Analytes PFDoA PFDoA PFDoA, PFOSA PFDoA PFDoA PFDoA PFDoA PFDoA PFDoA Perfluorochemicals in Mississippi River Pool 2 May 2013 31 Minnesota Pollution Control Agency CLIENT ID 123325 123326 123327 123328 123329 123330 123331 123332 123333 123334 123335 123336 123337 123338 123339 123340 123341 123342 123343 123344 123345 123346 123347 123348 123349 123350 123351 123352 123353 123354 123355 WORK GROUP WG41079 WG41079 WG41079 WG41079 WG41079 WG41079 WG41079 WG41079 WG41079 WG41079 WG41079 WG41079 WG41079 WG41077 WG41077 WG41077 WG41077 WG41077 WG41077 WG41077 WG41077 WG41077 WG41077 WG41077 WG41077 WG41078 WG41078 WG41078 WG41078 WG41078 WG41078 Sample (g - wet) 1.97 2.05 2.01 1.97 1.99 1.95 1.96 2.00 2.02 2.03 2.06 1.96 1.95 2.01 2.01 2.02 2.07 2.03 2.00 2.01 2.04 1.99 1.99 2.05 2.02 1.95 2.00 2.02 2.00 2.08 2.02 PFBA < 0.508 < 0.488 < 0.498 < 0.508 < 0.503 < 0.513 < 0.510 < 0.500 < 0.495 < 0.493 < 0.485 < 0.510 < 0.513 < 0.498 < 0.498 < 0.495 < 0.483 < 0.493 < 0.500 < 0.498 < 0.490 < 0.503 < 0.503 < 0.488 < 0.495 < 0.513 < 0.500 < 0.495 < 0.500 < 0.481 < 0.495 PFPeA < 0.508 < 0.488 < 0.498 < 0.508 < 0.503 < 0.513 < 0.510 < 0.500 < 0.495 < 0.493 < 0.485 < 0.510 < 0.513 < 0.498 < 0.498 < 0.495 < 0.483 < 0.493 < 0.500 < 0.498 < 0.490 < 0.503 < 0.503 < 0.488 < 0.495 < 0.513 < 0.500 < 0.495 < 0.500 < 0.481 < 0.495 PFHxA < 0.508 < 0.488 < 0.498 < 0.508 < 0.503 < 0.513 < 0.510 < 0.500 < 0.495 < 0.493 < 0.485 < 0.510 < 0.513 < 0.498 < 0.498 < 0.495 < 0.483 < 0.493 < 0.500 < 0.498 < 0.490 < 0.503 < 0.503 < 0.488 < 0.495 < 0.513 < 0.500 < 0.495 < 0.500 < 0.481 < 0.495 PFHpA < 0.508 < 0.488 < 0.498 < 0.508 < 0.503 < 0.513 < 0.510 < 0.500 < 0.495 < 0.493 < 0.485 < 0.510 < 0.513 < 0.498 < 0.498 < 0.495 < 0.483 < 0.493 < 0.500 < 0.498 < 0.490 < 0.503 < 0.503 < 0.488 < 0.495 < 0.513 < 0.500 < 0.495 < 0.500 < 0.481 < 0.495 PFOA < 0.508 < 0.488 < 0.498 < 0.508 < 0.503 < 0.513 < 0.510 < 0.500 < 0.495 < 0.493 < 0.485 < 0.510 < 0.513 < 0.498 < 0.498 < 0.495 < 0.483 < 0.493 < 0.500 < 0.498 < 0.490 < 0.503 < 0.503 < 0.488 < 0.495 < 0.513 < 0.500 < 0.495 < 0.500 < 0.481 < 0.495 PFNA < 0.508 < 0.488 < 0.498 0.742 < 0.503 < 0.513 0.574 0.802 1.16 < 0.493 < 0.485 < 0.510 < 0.513 0.518 0.557 0.971 0.643 0.653 < 0.500 < 0.498 < 0.490 < 0.503 0.76 1.2 < 0.495 0.625 0.595 0.842 < 0.500 < 0.481 < 0.495 PFDA 1.06 1.94 0.853 1.3 1.3 1.5 1.79 3.77 2.89 2.31 1.01 1.03 1.69 4.35 3.7 4.37 3.09 2.44 1.95 1.85 1.2 1.7 1.43 2.57 1.29 2.57 1.69 1.14 1.49 1.0 1.91 PFUnA 0.847 1.32 2.47 0.609 0.799 0.928 1.11 2.19 1.74 1.23 0.625 0.649 1.27 1.61 1.3 1.64 1.29 0.783 1.14 0.961 0.693 0.814 0.573 1.07 0.655 1.38 0.997 0.565 1.26 0.804 1.85 PFDoA 0.83 0.865 < 0.498 < 0.508 0.554 0.885 0.676 1.86 1.4 0.949 < 0.485 < 0.510 0.875 9.5 2.27 1.95 2.11 1.19 1.37 1.26 0.836 0.999 0.753 1.86 1.0 1.12 1.27 < 0.495 2.12 1.37 2.7 PFBS < 1.02 < 0.976 < 0.995 < 1.02 < 1.01 < 1.03 < 1.02 < 1.00 < 0.990 < 0.985 < 0.971 < 1.02 < 1.03 < 0.995 < 0.995 < 0.990 < 0.966 < 0.985 < 1.00 < 0.995 < 0.980 < 1.01 < 1.01 < 0.976 < 0.990 < 1.03 < 1.00 < 0.990 < 1.00 < 0.962 < 0.990 PFHxS < 1.02 < 0.976 < 0.995 < 1.02 < 1.01 < 1.03 < 1.02 < 1.00 < 0.990 < 0.985 < 0.971 < 1.02 < 1.03 < 0.995 < 0.995 < 0.990 < 0.966 < 0.985 < 1.00 < 0.995 < 0.980 < 1.01 < 1.01 < 0.976 < 0.990 < 1.03 < 1.00 < 0.990 < 1.00 < 0.962 < 0.990 PFOS 20.4 44.2 50.4 32.8 37.9 24.5 20.4 62.7 42.8 52.8 17.9 22.7 29.1 32.8 71.9 61 46.8 29.2 37.8 23.3 18.3 23.4 21.8 60.1 25.1 65 31.8 14.5 35.9 7.62 12.3 PFOSA < 0.609 < 0.585 < 0.597 < 0.609 1.62 < 0.615 0.826 < 0.600 0.668 1.31 < 0.583 < 0.612 < 0.615 1.29 < 0.597 1.46 0.908 < 0.591 < 0.600 < 0.597 < 0.588 < 0.603 < 0.603 < 0.585 < 0.594 < 0.615 < 0.600 < 0.594 < 0.600 < 0.577 < 0.594 QA Review Date 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/11/2012 10/11/2012 10/11/2012 10/11/2012 10/11/2012 10/11/2012 10/11/2012 10/11/2012 10/11/2012 10/11/2012 10/11/2012 10/11/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 Qualified Analytes PFDoA PFDoA PFDoA PFDoA PFDoA PFDoA PFDoA Perfluorochemicals in Mississippi River Pool 2 May 2013 32 Minnesota Pollution Control Agency CLIENT ID 123356 123357 123358 123359 123360 123361 123362 123363 123364 123365 123366 123367 123368 123369 123370 123371 123372 123373 123374 WORK GROUP WG41078 WG41078 WG41078 WG41078 WG41078 WG41078 WG41078 WG41078 WG41078 WG41078 WG41790 WG41790 WG41790 WG41790 WG41790 WG41790 WG41790 WG41790 WG41790 Sample (g - wet) 2.04 2.06 1.99 2.02 2.00 2.05 2.08 2.06 2.09 2.04 2.08 2.02 2.04 2.02 2.00 2.00 2.00 1.99 1.99 PFBA < 0.490 < 0.485 < 0.503 < 0.495 < 0.500 < 0.488 < 0.481 < 0.485 < 0.478 < 0.490 < 0.481 < 0.495 < 0.490 < 0.495 < 0.500 < 0.500 < 0.500 < 0.503 < 0.503 PFPeA < 0.490 < 0.485 < 0.503 < 0.495 < 0.500 < 0.488 < 0.481 < 0.485 < 0.478 < 0.490 < 0.481 < 0.495 < 0.490 < 0.495 < 0.500 < 0.500 < 0.500 < 0.503 < 0.503 PFHxA < 0.490 < 0.485 < 0.503 < 0.495 < 0.500 < 0.488 < 0.481 < 0.485 < 0.478 < 0.490 < 0.481 < 0.495 < 0.490 < 0.495 < 0.500 < 0.500 < 0.500 < 0.503 < 0.503 PFHpA < 0.490 < 0.485 < 0.503 < 0.495 < 0.500 < 0.488 < 0.481 < 0.485 < 0.478 < 0.490 < 0.481 < 0.495 < 0.490 < 0.495 < 0.500 < 0.500 < 0.500 < 0.503 < 0.503 PFOA < 0.490 < 0.485 < 0.503 < 0.495 < 0.500 < 0.488 < 0.481 < 0.485 < 0.478 < 0.490 < 0.481 < 0.495 < 0.490 < 0.495 < 0.500 < 0.500 < 0.500 < 0.503 < 0.503 PFNA < 0.490 < 0.485 < 0.503 < 0.495 < 0.500 < 0.488 < 0.481 < 0.485 < 0.478 < 0.490 1.0 < 0.495 0.656 0.631 < 0.500 < 0.500 < 0.500 0.523 1.14 PFDA 1.02 1.08 1.44 1.15 1.48 0.713 2.03 0.997 1.73 0.785 3.56 3 2.29 1.6 0.812 1.15 1.04 2.67 4.28 PFUnA 0.672 0.716 1.34 1.02 0.907 < 0.488 1.55 0.807 1.49 0.885 1.66 1.57 0.877 0.716 0.874 0.816 1.1 1.19 1.92 PFDoA 0.765 1.08 1.43 1.0 0.845 < 0.488 1.43 0.744 1.39 0.759 1.48 1.17 0.861 0.585 0.524 0.548 0.797 0.665 1.39 PFBS < 0.980 < 0.971 < 1.01 < 0.990 < 1.00 < 0.976 < 0.962 < 0.971 < 0.957 < 0.980 < 0.962 < 0.990 < 0.980 < 0.990 < 1.00 < 1.00 < 1.00 < 1.01 < 1.01 PFHxS < 0.980 < 0.971 < 1.01 < 0.990 < 1.00 < 0.976 < 0.962 < 0.971 < 0.957 < 0.980 < 0.962 < 0.990 < 0.980 < 0.990 < 1.00 < 1.00 < 1.00 < 1.01 < 1.01 PFOS 31 21.8 25.5 21.2 32.8 19.7 43.5 22.7 30.3 21.9 38.7 34.5 46.4 21.4 21.5 25.7 70.4 31.5 75.6 PFOSA < 0.588 < 0.583 < 0.603 < 0.594 < 0.600 < 0.585 < 0.577 < 0.583 < 0.574 < 0.588 < 0.577 < 0.594 0.715 < 0.594 < 0.600 < 0.600 < 0.600 < 0.603 1.04 QA Review Date 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 10/19/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 Qualified Analytes Perfluorochemicals in Mississippi River Pool 2 May 2013 33 Minnesota Pollution Control Agency Appendix A-2. PFCs results for water (ng/L) CLIENT ID 1A 2A 2B 2C 3A 3B 3C 4A 4B 4C 5A 6A 6B 6C 7A 7B 7C 8A WORK GROUP WG41442 WG41443 WG41443 WG41442 WG41442 WG41442 WG41442 WG41442 WG41442 WG41442 WG41443 WG41442 WG41442 WG41442 WG41443 WG41442 WG41442 WG41443 Sample (g - wet) 0.501 L 0.517 L 0.518 L 0.509 L 0.511 L 0.508 L 0.508 L 0.500 L 0.501 L 0.516 L 0.494 L 0.501 L 0.503 L 0.492 L 0.498 L 0.513 L 0.512 L 0.508 L PFBA 9.78 10.9 9.21 10.5 10.8 10.4 9.66 9.08 10.4 9.81 13.6 21.3 19.4 19.7 30 29.5 26.4 37.8 PFPeA < 2.50 < 2.42 < 2.41 < 2.46 < 2.45 < 2.46 < 2.46 < 2.50 < 2.49 < 2.42 < 2.53 2.85 < 2.49 < 2.54 < 2.51 2.89 2.52 3.55 8B WG41443 0.520 L 34.9 3.11 8C WG41443 0.510 L 36 3.37 9A WG41443 0.512 L 42.8 4.24 9B WG41443 0.473 L 44.7 4.05 9C WG41443 0.492 L 40.9 4.13 10A WG41443 0.551 L 47.8 4.05 10B WG41443 0.483 L 48.3 4.56 10C WG41443 0.472 L 50.7 4.34 11A WG41443 0.490 L 300 25.3 PFHxA < 2.50 < 2.42 < 2.41 < 2.46 < 2.45 < 2.46 < 2.46 < 2.50 < 2.49 < 2.42 < 2.53 4.35 4.22 4.47 4.74 4.21 4.05 5.08 5.02 5.29 5.93 6.54 6.83 6.33 6.01 6.56 32.8 PFHpA < 2.50 < 2.42 < 2.41 < 2.46 < 2.45 < 2.46 < 2.46 < 2.50 < 2.49 < 2.42 < 2.53 < 2.49 2.64 < 2.54 < 2.51 < 2.44 < 2.44 2.76 < 2.40 3.1 4.34 3.98 4.94 4.8 5.56 3.96 15.5 PFOA < 2.50 < 2.42 < 2.41 < 2.46 < 2.45 < 2.46 < 2.46 < 2.50 < 2.49 < 2.42 3.58 5.02 5.23 5.29 7.69 7.59 7.55 8.29 8.3 8.39 8.26 8.03 8.45 8.89 8.76 8.51 210 PFNA < 2.50 < 2.42 < 2.41 < 2.46 < 2.45 < 2.46 < 2.46 < 2.50 < 2.49 < 2.42 < 2.53 < 2.49 < 2.49 < 2.54 < 2.51 < 2.44 < 2.44 < 2.46 < 2.40 3.55 < 2.44 < 2.64 < 2.54 < 2.27 < 2.59 < 2.65 < 2.55 PFDA < 2.50 < 2.42 < 2.41 < 2.46 < 2.45 < 2.46 < 2.46 < 2.50 < 2.49 < 2.42 < 2.53 < 2.49 < 2.49 < 2.54 < 2.51 < 2.44 < 2.44 < 2.46 < 2.40 15.7 < 2.44 < 2.64 < 2.54 < 2.27 < 2.59 < 2.65 < 2.55 PFUnA < 2.50 < 2.42 < 2.41 < 2.46 < 2.45 < 2.46 < 2.46 < 2.50 < 2.49 < 2.42 < 2.53 < 2.49 < 2.49 < 2.54 < 2.51 < 2.44 < 2.44 < 2.46 < 2.40 60.2 < 2.44 < 2.64 < 2.54 < 2.27 < 2.59 < 2.65 < 2.55 PFDoA < 2.50 < 2.42 < 2.41 < 2.46 < 2.45 < 2.46 < 2.46 < 2.50 < 2.49 < 2.42 < 2.53 < 2.49 < 2.49 < 2.54 < 2.51 < 2.44 < 2.44 < 2.46 < 2.40 117 < 2.44 < 2.64 < 2.54 < 2.27 < 2.59 < 2.65 < 2.55 PFBS < 4.99 < 4.84 < 4.83 < 4.91 < 4.90 < 4.92 < 4.93 < 5.00 < 4.99 < 4.85 < 5.06 < 4.99 < 4.97 < 5.08 < 5.02 < 4.88 < 4.88 < 4.92 < 4.81 < 4.90 < 4.89 < 5.28 < 5.08 < 4.54 < 5.18 < 5.29 31 PFHxS < 4.99 < 4.84 < 4.83 < 4.91 < 4.90 < 4.92 < 4.93 < 5.00 < 4.99 < 4.85 < 5.06 < 4.99 < 4.97 < 5.08 < 5.02 < 4.88 < 4.88 < 4.92 < 4.81 < 4.90 < 4.89 < 5.28 < 5.08 < 4.54 < 5.18 < 5.29 25.7 PFOS < 4.99 < 4.84 < 4.83 < 4.91 < 4.90 < 4.92 < 4.93 < 5.00 < 4.99 < 4.85 < 5.06 < 4.99 < 4.97 < 5.08 < 5.02 < 4.88 < 4.88 < 4.92 < 4.81 19.8 < 4.89 < 5.28 6.09 < 4.54 < 5.18 < 5.29 150 PFOSA < 2.50 < 2.42 < 2.41 < 2.46 < 2.45 < 2.46 < 2.46 < 2.50 < 2.49 < 2.42 < 2.53 < 2.49 < 2.49 < 2.54 < 2.51 < 2.44 < 2.44 < 2.46 < 2.40 208 < 2.44 < 2.64 < 2.54 < 2.27 < 2.59 < 2.65 < 2.55 QA Review Date 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 Qualified Analytes PFBA PFBA PFBA PFBA PFBA PFBA PFBA PFBA PFBA PFBA PFBA PFBA PFBA PFBA PFBA PFBA PFBA PFBA, PFPeA, PFHxA, PFHpA, PFHxA PFBA PFBA, PFUnA, PFDoA PFBA, PFPeA, PFHxA, PFHpA, PFHxA PFBA PFBA PFBA PFBA, PFPeA, PFHxA, PFHpA, PFHxA PFBA PFBA Perfluorochemicals in Mississippi River Pool 2 May 2013 34 Minnesota Pollution Control Agency CLIENT ID 11B 11C 12A 12B 12C WORK GROUP WG41443 WG41443 WG41443 WG41443 WG41443 Sample (g - wet) 0.458 L 0.490 L 0.517 L 0.512 L 0.489 L PFBA 309 326 187 187 183 PFPeA 23.4 26.7 12.1 12.5 9.78 PFHxA 31.4 34.2 12.6 12.5 11.8 PFHpA 15.1 17.4 5.34 5.09 5.87 PFOA 202 220 37.1 37.8 34.9 PFNA < 2.73 < 2.55 < 2.42 < 2.44 < 2.56 PFDA < 2.73 < 2.55 < 2.42 < 2.44 < 2.56 PFUnA < 2.73 < 2.55 < 2.42 < 2.44 < 2.56 PFDoA < 2.73 < 2.55 < 2.42 < 2.44 < 2.56 PFBS 33.4 30.3 13 13.6 13.4 PFHxS 24.2 26.4 13.9 14.4 14 PFOS 142 155 24.2 25.9 23.1 PFOSA < 2.73 < 2.55 < 2.42 < 2.44 < 2.56 QA Review Date 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 Qualified Analytes PFBA PFBA PFBA PFBA PFBA Perfluorochemicals in Mississippi River Pool 2 May 2013 35 Minnesota Pollution Control Agency Appendix A-3. QA Results for Fish Samples: lab blanks and matrix spikes by work group WORK GROUP WG40905 WG40905 WG40905 WG40905 WG40904 WG40904 WG40904 WG40904 WG40903 WG40903 WG40903 WG40903 WG40901 WG40901 WG40901 WG40901 WG40901 WG40901 WG40902 WG40902 WG40902 WG40902 WG40898 WG40898 WG40898 WG40898 WG40906 WG40906 WG40906 WG40906 CLIENT ID Lab Blank Spiked Matrix 123255 (MS) 123255 (MSD) Lab Blank Spiked Matrix 123172 (MS) 123172 (MSD) Lab Blank Spiked Matrix 123121 (MS) 123121 (MSD) Lab Blank Spiked Matrix 123077 (MS) 123077 (MS) 123077 (MSD) 123077 (MSD) Lab Blank Spiked Matrix 123105 (MS) 123105 (MSD) Lab Blank Spiked Matrix 123152 (MS) 123152 (MSD) Lab Blank Spiked Matrix 123278 (MS) 123278 (MSD) Sample (g - wet) 2.00 2.01 1.99 2.00 2.00 2.00 2.00 2.00 2.05 2.00 2.00 2.00 2.00 2.00 2.00 2.04 2.02 2.00 1.99 2.00 2.00 2.04 1.98 UNITS ng/g % Recov % Recov % Recov ng/g % Recov % Recov % Recov ng/g % Recov % Recov % Recov ng/g % Recov ng ww % Recov ng ww % Recov ng/g % Recov ng/g ww ng/g ww ng/g % Recov ng/g ww ng/g ww ng/g % Recov % Recov % Recov PFBA < 0.500 95.2 94.5 97.4 < 0.500 107 99.1 99.4 < 0.500 123 115 118 < 0.500 113 22 110 22.2 111 < 0.500 101 19.6 20.7 < 0.500 102 22.7 22.6 < 0.500 97.3 93.9 92 PFPeA < 0.500 96.8 108 131 < 0.500 95 146 162 < 0.500 107 111 113 < 0.500 99.6 23.3 116 23.6 118 < 0.500 94.3 17.1 17.6 < 0.500 96.1 21.2 21 < 0.500 96.5 119 128 PFHxA < 0.500 105 101 97.6 < 0.500 104 107 112 < 0.500 124 125 127 < 0.500 112 23 115 22.9 114 < 0.500 106 20.7 21.2 < 0.500 108 24.1 23.6 < 0.500 104 104 102 PFHpA < 0.500 100 95.3 107 < 0.500 97 120 129 < 0.500 117 118 115 < 0.500 105 24.2 121 22.9 115 < 0.500 98.4 16.6 18.5 < 0.500 102 21.4 20.6 < 0.500 96.4 105 107 PFOA < 0.500 97.7 101 95.4 < 0.500 101 109 109 < 0.500 122 128 122 < 0.500 106 22 110 23 115 < 0.500 102 20.4 21.2 < 0.500 106 23.1 22.3 < 0.500 101 103 99.8 PFNA < 0.500 91.3 99.4 96.1 < 0.500 96.2 96.7 105 < 0.500 122 116 117 < 0.500 111 22 110 21.1 106 < 0.500 99.3 20.3 20.1 < 0.500 105 22.9 23.7 < 0.500 104 96.2 105 PFDA < 0.500 104 106 103 < 0.500 116 117 118 < 0.500 128 134 132 < 0.500 126 22.2 111 26.1 131 < 0.500 114 24 22.5 < 0.500 114 28.9 29.6 < 0.500 105 116 108 PFUnA < 0.500 78.3 79.4 84.1 < 0.500 104 79.8 88 < 0.500 113 100 105 < 0.500 110 15.7 78.3 21.1 106 < 0.500 105 21.4 20.1 < 0.500 112 24.1 27.2 < 0.500 92.5 97.9 99.5 PFDoA < 0.500 104 98.6 97.5 < 0.500 105 106 108 < 0.500 124 129 127 < 0.500 110 22.7 113 22.3 111 < 0.500 101 21.9 22.7 < 0.500 113 26.2 26.8 < 0.500 104 106 101 PFBS < 1.00 101 90.3 70.3 < 1.00 107 65.4 68.8 < 1.00 114 122 126 < 1.00 122 38.3 95.8 37.7 94.2 < 1.00 118 43.5 43.1 < 1.00 106 45 46.6 < 1.00 101 78.7 72.5 PFHxS < 1.00 96.5 98.6 95.6 < 1.00 100 95 101 < 1.00 111 114 118 < 1.00 105 41.3 103 43.2 108 < 1.00 95.9 39.1 37.6 < 1.00 99.1 42.2 42.4 < 1.00 100 97 94.6 PFOS < 1.00 92.8 83.1 85.2 < 1.00 91.9 98.8 88.8 < 1.00 120 126 116 < 1.00 108 46.6 114 42.3 103 < 1.00 94 90.2 83.8 < 1.00 101 120 115 < 1.00 98.2 108 101 PFOSA < 0.600 95.9 103 110 < 0.600 98.5 113 115 < 0.600 125 143 131 < 0.600 109 27.1 135 25.9 129 < 0.600 102 22.2 21.4 < 0.500 111 23.4 24 < 0.600 104 108 107 Perfluorochemicals in Mississippi River Pool 2 May 2013 36 Minnesota Pollution Control Agency WORK GROUP WG40910 WG40910 WG40910 WG40910 WG40910 WG40910 WG40909 WG40909 WG40909 WG40909 WG40909 WG40909 WG40908 WG40908 WG40908 WG40908 WG40908 WG40908 WG40907 WG40907 WG40907 WG40907 WG40907 WG40907 WG40911 WG40911 WG40911 WG40911 WG40911 WG40911 WG41230 CLIENT ID Lab Blank Spiked Matrix 123190 (MS) 123190 (MSD) 123190 (MSD) 123190 (MS) Lab Blank Spiked Matrix 123291 (MS) 123291 (MSD) 123291 (MSD) 123291 (MS) Lab Blank Spiked Matrix 123229 (MS) 123229 (MSD) 123229 (MSD) 123229 (MS) Lab Blank Spiked Matrix 123205 (MS) 123205 (MSD) 123205 (MSD) 123205 (MS) Lab Blank Spiked Matrix 123142 (MS) 123142 (MSD) 123142 (MSD) 123142 (MS) Lab Blank Sample (g - wet) 2.00 2.07 2.04 2.04 2.07 2.00 2.09 2.05 2.05 2.09 2.00 1.99 2.03 2.03 1.99 2.00 2.00 1.98 1.98 2.00 2.00 2.04 1.99 1.99 2.04 2.00 UNITS ng/g % Recov ng ww ng ww % Recov % Recov ng/g % Recov ng ww ng ww % Recov % Recov ng/g % Recov ng ww ng ww % Recov % Recov ng/g % Recov ng/g ww ng/g ww % Recov % Recov ng/g % Recov ng ww ng ww % Recov % Recov ng/g PFBA < 0.500 110 21 19 96.9 109 < 0.500 113 23.3 21.8 112 122 < 0.500 115 22.9 22.4 114 114 < 0.500 95.3 41.2 46.8 109 82.3 < 0.500 96.2 18.9 17.3 86.2 96.4 < 0.500 PFPeA < 0.500 111 40.5 39.4 201 209 < 0.500 123 31.6 33.8 173 165 < 0.500 129 45.7 46.5 236 227 < 0.500 103 26.8 24.5 118 130 < 0.500 104 27.3 28.2 140 139 < 0.500 PFHxA < 0.500 120 23.8 24.7 126 123 < 0.500 126 23.1 26.4 135 121 < 0.500 127 25.6 24.2 123 128 < 0.500 98.4 21.3 21.7 107 107 < 0.500 104 19.6 21.2 106 100 < 0.500 PFHpA < 0.500 113 22.5 25.5 130 116 < 0.500 108 23.1 26.3 135 121 < 0.500 119 23.3 23.2 118 116 < 0.500 82 20.8 22.5 111 104 < 0.500 100 19.6 20.8 104 100 < 0.500 PFOA < 0.500 121 23.2 22.5 115 120 < 0.500 129 24.4 24.7 127 128 < 0.500 127 26.8 23.5 119 133 < 0.500 97.1 30.3 28.9 98.9 107 < 0.500 99.1 20 21 104 102 < 0.500 PFNA < 0.500 118 23.6 21.4 109 122 < 0.500 119 22.2 22.9 117 116 < 0.500 120 24.7 23.1 114 120 < 0.500 91.1 19.3 18.1 87 94.1 < 0.500 99.2 18.5 19.6 97.3 94.3 < 0.500 PFDA < 0.500 129 24.4 28.3 137 118 < 0.500 123 26.5 26.6 130 132 < 0.500 137 36.1 36 139 137 < 0.500 110 30.9 31.5 99.1 96.8 < 0.500 98.8 22.6 21.6 105 113 < 0.500 PFUnA < 0.500 113 20.7 22.5 105 97.7 < 0.500 108 20.7 21.4 105 103 < 0.500 116 24.9 22.7 97.3 107 < 0.500 98.7 19.1 22.9 96.4 78.5 < 0.500 95.5 17.4 16.5 82 89 < 0.500 PFDoA < 0.500 121 25.1 26.2 123 119 < 0.500 130 26.1 24.8 120 128 < 0.500 126 26.9 27.1 124 121 < 0.500 98.2 26.5 25.3 102 109 < 0.500 109 21.6 22 109 110 < 0.500 PFBS < 1.00 121 27.2 32.9 84 70.3 < 1.00 133 33.8 42.5 109 88.4 < 1.00 120 38.7 37.1 94.1 96.3 < 1.00 99.8 22 38.6 95.6 55.1 < 1.00 85.2 25.3 25.9 64.3 64.5 < 1.00 PFHxS < 1.00 119 38.8 48 122 100 < 1.00 125 44.4 53.2 136 116 < 1.00 112 49.7 52.3 133 124 < 1.00 96 49.4 64 126 90.4 < 1.00 84.1 34.4 36.3 90.3 87.6 < 1.00 PFOS < 1.00 99.5 104 108 154 148 < 1.00 119 62.1 69.1 131 115 < 1.00 111 134 141 148 128 < 1.00 110 5730 5460 < 1.00 94.9 60.4 65.3 92 81.8 < 1.00 PFOSA < 0.600 118 22.4 23.3 115 112 < 0.600 116 23.3 26 133 122 < 0.600 112 25 25 127 124 < 0.600 103 43.4 43.6 119 120 < 0.600 97 23.7 22.2 111 121 < 0.600 Perfluorochemicals in Mississippi River Pool 2 May 2013 37 Minnesota Pollution Control Agency WORK GROUP WG41230 WG41230 WG41230 WG41230 WG41230 WG41077 WG41077 WG41077 WG41077 WG41155 WG41155 WG41079 WG41079 WG41079 WG41079 WG41790 WG41790 WG41790 WG41790 CLIENT ID Spiked Matrix 123300 (MS) 123300 (MS) 123300 (MSD) 123300 (MSD) Lab Blank Spiked Matrix 123306 (MS) 123306 (MSD) Lab Blank Spiked Matrix Lab Blank Spiked Matrix 123334 (MS) 123334 (MSD) Lab Blank Spiked Matrix 123368 (MS) 123368 (MSD) Sample (g - wet) 1.99 1.99 2.06 2.06 2.00 2.02 2.01 2.00 2.00 2.04 2.03 2.00 2.00 2.09 UNITS % Recov ng/g ww % Recov ng/g ww % Recov ng/g % Recov ng ww ng ww ng/g % Recov ng/g % Recov % Recov % Recov ng/g % Recov % Recov % Recov PFBA 107 20.6 103 18.8 97 < 0.500 95.4 20.3 21.3 < 2.50 94.2 < 0.500 88.3 93.2 97.1 < 0.500 112 112 109 PFPeA 111 29.1 145 29.1 150 < 0.500 115 32.6 34.4 < 2.50 92.2 < 0.500 81.9 93 84.3 < 0.500 94 99.4 87.8 PFHxA 109 20.6 103 19.8 102 < 0.500 106 20.3 21.4 < 2.50 97 < 0.500 96.2 96.6 94.5 < 0.500 112 118 106 PFHpA 107 22.3 111 22 113 < 0.500 98.3 22 22.7 < 2.50 93.4 < 0.500 85.5 66.9 72.7 < 0.500 106 103 90.5 PFOA 99.5 22.9 114 21 108 < 0.500 111 23.6 22.6 < 2.50 104 < 0.500 93.7 93 87.9 < 0.500 107 109 101 PFNA 102 22 109 20.1 103 < 0.500 106 21.8 21.4 < 2.50 94.6 < 0.500 87 92.8 94.3 < 0.500 107 110 95 PFDA 114 23.3 111 23.6 116 < 0.500 124 24.7 25.7 < 2.50 80.8 < 0.500 103 103 102 < 0.500 121 135 116 PFUnA 108 16.9 81.4 19 95.2 < 0.500 89.5 17.7 17.9 < 2.50 102 < 0.500 103 107 110 < 0.500 116 109 110 PFDoA 112 22.9 114 22.4 115 < 0.500 113 24.6 23.5 < 2.50 103 < 0.500 103 104 103 < 0.500 115 115 104 PFBS 106 28.7 71.4 21.8 56.1 < 1.00 124 31.3 33.9 < 5.00 98.3 < 1.00 91.2 94.5 99.2 < 1.00 111 111 99.2 PFHxS 103 39.1 97.2 33.3 85.9 < 1.00 110 39.7 47.5 < 5.00 91.5 < 1.00 90.4 95.8 93.9 < 1.00 112 111 99.3 PFOS 105 84.9 106 78.6 93.6 < 1.00 95.1 84.8 80.6 < 5.00 89.9 < 1.00 89.5 118 91.6 < 1.00 118 98.3 86.1 PFOSA 95.9 25.5 127 21.5 110 < 0.600 121 27.1 28.6 < 2.50 98.1 < 0.600 91.1 88.8 81.7 < 0.600 109 113 94.8 Perfluorochemicals in Mississippi River Pool 2 May 2013 38 Minnesota Pollution Control Agency Appendix A-4. QA Results for Water Samples: lab blanks and matrix spikes by work group WORK GROUP WG41443 WG41443 WG41443 WG41443 WG41442 WG41442 WG41442 WG41442 CLIENT ID Lab Blank Spiked Matrix 5A (MS) 5A (MSD) Lab Blank Spiked Matrix 1A (MS) 1A (MSD) Sample Size (L) 0.500 0.504 0.539 0.500 0.509 0.500 UNITS ng/L % Recov % Recov % Recov ng/L % Recov ng/L ng/L PFBA < 2.50 123 113 130 < 2.50 97.6 492 527 PFPeA < 2.50 105 79.9 93.2 < 2.50 90.8 377 417 PFHxA < 2.50 120 105 124 < 2.50 108 503 541 PFHpA < 2.50 114 130 154 < 2.50 97.6 623 690 PFOA < 2.50 113 105 116 < 2.50 102 483 497 PFNA < 2.50 113 105 116 < 2.50 102 468 502 PFDA < 2.50 116 100 113 < 2.50 104 470 517 PFUnA < 2.50 122 107 120 < 2.50 109 473 512 PFDoA < 2.50 123 104 112 < 2.50 106 481 525 PFBS < 5.00 115 92.9 97.6 < 5.00 91.3 788 844 PFHxS < 5.00 117 109 120 < 5.00 84.3 905 983 PFOS < 5.00 106 108 120 < 5.00 107 935 1010 PFOSA < 2.50 119 119 130 < 2.50 113 496 557 Perfluorochemicals in Mississippi River Pool 2 May 2013 39 Minnesota Pollution Control Agency Appendix A-5. Fish sample identification MPCA ID Section Run Sample Date Species 12 3076 1 1 7/30/2012 Common Carp 12 3077 1 1 7/30/2012 Common Carp 12 3078 1 1 7/30/2012 Common Carp 12 3079 1 1 7/30/2012 Common Carp 12 3080 1 1 7/30/2012 Common Carp 12 3081 1 1 7/30/2012 Common Carp 12 3082 1 1 7/30/2012 Common Carp 12 3083 1 1 7/30/2012 Smallmouth Bass 12 3084 1 1 7/30/2012 Smallmouth Bass 12 3085 1 1 7/30/2012 Smallmouth Bass 12 3086 1 1 7/30/2012 Smallmouth Bass 12 3087 1 1 7/30/2012 Smallmouth Bass 12 3088 1 1 7/30/2012 Smallmouth Bass 12 3089 1 1 7/30/2012 Smallmouth Bass 12 3090 1 1 7/30/2012 Smallmouth Bass 12 3091 1 1 7/30/2012 Smallmouth Bass 12 3092 1 1 7/30/2012 Smallmouth Bass 12 3093 1 1 7/30/2012 Smallmouth Bass 12 3094 1 1 7/30/2012 Freshwater Drum 12 3095 1 1 7/30/2012 Freshwater Drum 12 3096 1 1 7/30/2012 Freshwater Drum 12 3097 1 1 7/30/2012 Freshwater Drum 12 3098 1 1 7/30/2012 Freshwater Drum 12 3099 1 1 7/30/2012 Freshwater Drum 12 3100 1 1 7/30/2012 Freshwater Drum 12 3101 1 1 7/30/2012 Freshwater Drum 12 3102 1 1 7/30/2012 Freshwater Drum Perfluorochemicals in Mississippi River Pool 2 May 2013 Length Sex Age (cm) Weight (g) AXYS ID Sample Received Work Group m 8 65 3500 L18329-1 8-Aug-12 WG40901 m 8 62 3950 L18329-2 (A) 8-Aug-12 WG40901 m 8 62 1275 L18329-3 8-Aug-12 WG40901 ind 5 45 1300 L18329-4 8-Aug-12 WG40901 f 6 52 2500 L18329-5 8-Aug-12 WG40901 f 7 57.5 2750 L18329-6 8-Aug-12 WG40901 f 8 60.5 2600 L18329-7 8-Aug-12 WG40901 m 7 37 956 L18330-1 7-Aug-12 WG40901 m 6 34 832 L18330-2 7-Aug-12 WG40901 m 6 32 451 L18330-3 7-Aug-12 WG40901 ind 5 33.5 572 L18330-4 7-Aug-12 WG40901 f 6 34.5 716 L18330-5 7-Aug-12 WG40901 m 7 38 950 L18330-6 7-Aug-12 WG40901 f 4 29 377 L18330-7 7-Aug-12 WG40901 m 7 34 684 L18330-8 7-Aug-12 WG40901 f 4 30.5 432 L18330-9 7-Aug-12 WG40901 f 3 29 402 L18330-10 7-Aug-12 WG40901 j 3 23.3 205 L18330-11 7-Aug-12 WG40901 f 5 34 483 L18330-12 7-Aug-12 WG40901 m 6 37 719 L18330-13 7-Aug-12 WG40902 m 8 42 862 L18330-14 7-Aug-12 WG40902 f 5 34 413 L18330-15 7-Aug-12 WG40902 m 4 29.5 308 L18330-16 7-Aug-12 WG40902 m 8 39.5 671 L18330-17 7-Aug-12 WG40902 m 3 31.5 348 L18330-18 7-Aug-12 WG40902 m 3 29 284 L18330-19 7-Aug-12 WG40902 m 7 38.5 617 L18330-20 7-Aug-12 WG40902 40 Minnesota Pollution Control Agency MPCA ID Section Run Sample Date Species Length Sex Age (cm) Weight (g) AXYS ID Sample Received Work Group 12 3103 1 1 7/30/2012 Freshwater Drum ind 4 33 372 L18330-21 7-Aug-12 WG40902 12 3104 1 1 7/30/2012 Freshwater Drum f 4 31 258 L18330-22 7-Aug-12 WG40902 12 3105 1 1 7/30/2012 Freshwater Drum f 5 34 422 L18330-23 (A) 7-Aug-12 WG40902 12 3106 1 1 7/30/2012 Bluegill Sunfish m 5 15.5 98 L18330-24 7-Aug-12 WG40902 12 3107 1 1 7/30/2012 Bluegill Sunfish ind 5 15.5 115 L18330-25 7-Aug-12 WG40902 12 3108 1 1 7/30/2012 Bluegill Sunfish j 2 10 20.62 L18330-26 7-Aug-12 WG40902 12 3109 1 1 7/30/2012 Bluegill Sunfish f 5 15 87 L18330-27 7-Aug-12 WG40902 12 3110 1 1 7/30/2012 Bluegill Sunfish ind 5 15 74 L18330-28 7-Aug-12 WG40902 12 3111 1 1 7/30/2012 Bluegill Sunfish j 3 13.5 56 L18330-29 7-Aug-12 WG40902 12 3112 1 1 7/30/2012 Bluegill Sunfish j 3 12.5 45 L18330-30 7-Aug-12 WG40902 12 3113 1 1 7/30/2012 Bluegill Sunfish f 7 18.5 69 L18330-31 7-Aug-12 WG40902 12 3114 1 1 7/30/2012 Bluegill Sunfish m 4 14.5 175 L18330-32 7-Aug-12 WG40903 12 3115 1 1 7/30/2012 Bluegill Sunfish m 4 14 88 L18330-33 7-Aug-12 WG40903 12 3116 1 1 7/30/2012 Bluegill Sunfish m 4 14 64 L18330-34 7-Aug-12 WG40903 12 3117 1 1 7/30/2012 Bluegill Sunfish 1nd 4 14 59.14 L18330-35 7-Aug-12 WG40903 12 3118 1 1 7/30/2012 Bluegill Sunfish j 2 10.5 27.77 L18330-36 7-Aug-12 WG40903 12 3119 1 1 7/30/2012 Bluegill Sunfish f 2 12 56.34 L18330-37 7-Aug-12 WG40903 12 3120 1 1 7/30/2012 Bluegill Sunfish m 5 15.5 55.66 L18330-38 7-Aug-12 WG40903 12 3121 1 2 7/30/2012 Common Carp m 7 53.5 2643 L18330-39 (A) 7-Aug-12 WG40903 12 3122 1 2 7/30/2012 Common Carp m 7 55 3072 L18330-40 7-Aug-12 WG40903 12 3123 1 2 7/30/2012 Freshwater Drum m 4 35 540 L18330-41 7-Aug-12 WG40903 12 3124 1 2 7/30/2012 Freshwater Drum m 8 39.5 559 L18328-1 7-Aug-12 WG40898 12 3125 1 2 7/30/2012 Freshwater Drum f 5 33 433 L18328-2 7-Aug-12 WG40898 12 3126 1 3 7/30/2012 Common Carp f 8 59 3700 L18328-3 7-Aug-12 WG40898 12 3127 1 3 7/30/2012 Common Carp f 8 58.6 2900 L18328-4 7-Aug-12 WG40898 12 3128 1 3 7/30/2012 Common Carp f 8 57 2640 L18328-5 7-Aug-12 WG40898 12 3129 1 3 7/30/2012 Common Carp m 8 62 3500 L18328-6 7-Aug-12 WG40898 12 3130 1 3 7/30/2012 Smallmouth Bass m 3 24.5 205 L18328-7 7-Aug-12 WG40898 Perfluorochemicals in Mississippi River Pool 2 May 2013 41 Minnesota Pollution Control Agency MPCA ID Section Run Sample Date Species 12 3131 1 3 7/30/2012 Smallmouth Bass 12 3132 1 3 7/30/2012 Smallmouth Bass 12 3133 1 3 7/30/2012 White Bass 12 3134 1 4 7/30/2012 Common Carp 12 3135 1 4 7/30/2012 Common Carp 12 3136 1 4 7/30/2012 White Bass 12 3137 1 4 7/30/2012 White Bass 12 3138 1 4 7/30/2012 Smallmouth Bass 12 3139 1 5 7/30/2012 White Bass 12 3140 2 1 7/30/2012 Common Carp 12 3141 2 1 7/30/2012 Common Carp 12 3142 2 1 7/30/2012 Common Carp 12 3143 2 1 7/30/2012 Common Carp 12 3144 2 1 7/30/2012 Common Carp 12 3145 2 1 7/30/2012 Common Carp 12 3146 2 1 7/30/2012 White Bass 12 3147 2 1 7/30/2012 White Bass 12 3148 2 1 7/30/2012 White Bass 12 3149 2 1 7/30/2012 White Bass 12 3150 2 1 7/30/2012 White Bass 12 3151 2 1 7/30/2012 White Bass 12 3152 2 1 7/30/2012 White Bass 12 3153 2 1 7/30/2012 White Bass 12 3154 2 1 7/30/2012 White Bass 12 3155 2 1 7/30/2012 White Bass 12 3156 2 1 7/30/2012 White Bass 12 3157 2 1 7/30/2012 White Bass 12 3158 2 1 7/30/2012 White Bass Perfluorochemicals in Mississippi River Pool 2 May 2013 Length Sex Age (cm) Weight (g) AXYS ID Sample Received Work Group m 6 34 778 L18328-8 7-Aug-12 WG40898 m 2 22 167 L18328-9 7-Aug-12 WG40898 f 1 20 131 L18328-10 7-Aug-12 WG40898 m 7 57.5 2900 L18336-1 8-Aug-12 WG40911 m 7 53 2500 L18336-2 8-Aug-12 WG40911 m 3 31 493 L18336-3 8-Aug-12 WG40911 f 2 23 225 L18336-4 8-Aug-12 WG40911 f 8 36 721 L18336-5 8-Aug-12 WG40911 f 5 36 815 L18336-6 8-Aug-12 WG40911 f 7 54 2700 L18336-7 8-Aug-12 WG40911 m 7 53 2500 L18336-8 8-Aug-12 WG40911 m 7 55 3100 L18336-9 (A) 8-Aug-12 WG40911 j 3 32.5 624 L18336-10 8-Aug-12 WG40911 j 3 33 684 L18336-11 8-Aug-12 WG40911 f 5 36 2100 L18336-12 8-Aug-12 WG40911 f 6 50 820 L18336-13 8-Aug-12 WG40911 m 4 35 792 L18328-11 7-Aug-12 WG40898 f 4 33.5 686 L18328-12 7-Aug-12 WG40898 f 5 36 809 L18328-13 7-Aug-12 WG40898 m 2 29 417 L18328-14 7-Aug-12 WG40898 f 4 35 727 L18328-15 7-Aug-12 WG40898 m 3 31 494 L18328-16 (A) 7-Aug-12 WG40898 ind 3 29.5 416 L18328-17 7-Aug-12 WG40898 f 2 27.5 346 L18328-18 7-Aug-12 WG41155 m 4 35 714 L18328-19 7-Aug-12 WG40898 f 3 29.5 437 L18328-20 7-Aug-12 WG40898 j 1 20 141.82 L18331-1 8-Aug-12 WG40903 j 1 19 113.28 L18331-2 8-Aug-12 WG40903 42 Minnesota Pollution Control Agency MPCA ID Section Run Sample Date Species Length Sex Age (cm) Weight (g) AXYS ID Sample Received Work Group 12 3159 2 1 7/30/2012 White Bass j 1 21 143 L18331-3 8-Aug-12 WG40903 12 3160 2 1 7/30/2012 White Bass j 1 19 131 L18331-4 8-Aug-12 WG40903 12 3161 2 1 7/30/2012 Freshwater Drum m 4 30 303 L18331-5 8-Aug-12 WG40903 12 3162 2 1 7/30/2012 Freshwater Drum f 7 38 635 L18331-6 8-Aug-12 WG40903 12 3163 2 1 7/30/2012 Freshwater Drum m 9 43 1056 L18331-7 8-Aug-12 WG40903 12 3164 2 1 7/30/2012 Freshwater Drum m 6 36 523 L18331-8 8-Aug-12 WG40903 12 3165 2 1 7/30/2012 Freshwater Drum m 5 33 435 L18331-9 8-Aug-12 WG40903 12 3166 2 1 7/30/2012 Bluegill Sunfish m 5 15 71.56 L18331-10 8-Aug-12 WG40904 12 3167 4 1 7/31/2012 Common Carp m 6 49.5 1876 L18331-11 8-Aug-12 WG40904 12 3168 4 1 7/31/2012 Common Carp f 7 56 2350 L18331-12 8-Aug-12 WG40904 12 3169 4 1 7/31/2012 Common Carp m 3 34 682 L18331-13 8-Aug-12 WG40904 12 3170 4 1 7/31/2012 Common Carp m 5 43 1424 L18331-14 8-Aug-12 WG40904 12 3171 4 1 7/31/2012 Common Carp m 4 40 1004 L18331-15 8-Aug-12 WG40904 12 3172 4 1 7/31/2012 Common Carp m 6 52 2260 L18331-16 (A) 8-Aug-12 WG40904 12 3173 4 1 7/31/2012 Common Carp m 3 34 710 L18331-17 8-Aug-12 WG40904 12 3174 4 1 7/31/2012 Common Carp f 3 30 497 L18331-18 8-Aug-12 WG40904 12 3175 4 1 7/31/2012 Common Carp m 6 50 2072 L18331-19 8-Aug-12 WG40904 12 3176 4 1 7/31/2012 Freshwater Drum m 6 36 540 L18331-20 8-Aug-12 WG40904 12 3177 4 1 7/31/2012 Freshwater Drum m 12 51.5 1590 L18331-21 8-Aug-12 WG40904 12 3178 4 1 7/31/2012 Freshwater Drum m 5 33.5 425 L18331-22 8-Aug-12 WG40904 12 3179 4 1 7/31/2012 Freshwater Drum f 11 49 1430 L18335-1 8-Aug-12 WG41230 12 3180 4 1 7/31/2012 Freshwater Drum m 8 40 831 L18335-2 8-Aug-12 WG41230 12 3181 4 1 7/31/2012 Freshwater Drum m 5 34 439 L18335-3 8-Aug-12 WG41230 12 3182 4 1 7/31/2012 Freshwater Drum m 4 32 463 L18335-4 8-Aug-12 WG41230 12 3183 4 1 7/31/2012 Freshwater Drum f 8 40 794 L18335-5 8-Aug-12 WG41230 12 3184 4 1 7/31/2012 Freshwater Drum f 9 43 908 L18335-6 8-Aug-12 WG41230 12 3185 4 1 7/31/2012 White Bass f 7 39 956 L18335-7 8-Aug-12 WG41230 12 3186 4 1 7/31/2012 White Bass f 4 33 604 L18335-8 8-Aug-12 WG41230 Perfluorochemicals in Mississippi River Pool 2 May 2013 43 Minnesota Pollution Control Agency MPCA ID Section Run Sample Date Species Length Sex Age (cm) Weight (g) AXYS ID Sample Received Work Group 12 3187 4 1 7/31/2012 White Bass j 1 20 127 L18335-9 8-Aug-12 WG40910 12 3188 4 1 7/31/2012 White Bass m 4 35 731 L18335-10 8-Aug-12 WG40910 12 3189 4 1 7/31/2012 White Bass j 1 19.5 112 L18335-11 8-Aug-12 WG40910 12 3190 4 1 7/31/2012 Smallmouth Bass ind 5 31 522 L18335-12 (A) 8-Aug-12 WG40910 12 3191 4 1 7/31/2012 Smallmouth Bass ind 4 29 417 L18335-13 8-Aug-12 WG40910 12 3192 4 1 7/31/2012 Smallmouth Bass m 4 28 376 L18335-14 8-Aug-12 WG40910 12 3193 4 1 7/31/2012 Smallmouth Bass j 2 17 330 L18335-15 8-Aug-12 WG40910 12 3194 4 1 7/31/2012 Smallmouth Bass j 2 16 283 L18335-16 8-Aug-12 WG40910 12 3195 4 1 7/31/2012 Smallmouth Bass j 1 12.5 171 L18335-17 8-Aug-12 WG40910 12 3196 4 1 7/31/2012 Smallmouth Bass f 5 32 490 L18335-18 8-Aug-12 WG40910 12 3197 4 1 7/31/2012 Smallmouth Bass j 2 22.5 485 L18335-19 8-Aug-12 WG40910 12 3198 4 1 7/31/2012 Bluegill Sunfish j 5 15 91.9 L18335-20 8-Aug-12 WG40910 12 3199 4 1 7/31/2012 Bluegill Sunfish j 4 14 85.1 L18335-21 8-Aug-12 WG40910 12 3200 4 1 7/31/2012 Bluegill Sunfish f 2 12 45.88 L18335-22 8-Aug-12 WG40910 12 3201 4 1 7/31/2012 Bluegill Sunfish f 2 12 46.92 L18335-23 8-Aug-12 WG40910 12 3202 4 1 7/31/2012 Bluegill Sunfish f 5 15.5 101 L18335-24 8-Aug-12 WG40910 12 3203 4 1 7/31/2012 Bluegill Sunfish f 4 14 86.09 L18335-25 8-Aug-12 WG40910 12 3204 4 1 7/31/2012 Bluegill Sunfish j 2 11 29.15 L18335-26 8-Aug-12 WG40910 12 3205 4 2 7/31/2012 Common Carp m 7 53.5 2800 L18333-1 (A) 8-Aug-12 WG40907 12 3206 4 2 7/31/2012 Common Carp m 7 53 2100 L18333-2 8-Aug-12 WG40907 12 3207 4 2 7/31/2012 Common Carp m 7 56 2300 L18333-3 8-Aug-12 WG40907 12 3208 4 2 7/31/2012 Common Carp m 3 35 735 L18333-4 8-Aug-12 WG40907 12 3209 4 2 7/31/2012 Freshwater Drum m 8 42 932 L18333-5 8-Aug-12 WG40907 12 3210 4 2 7/31/2012 Freshwater Drum f 7 38 708 L18333-6 8-Aug-12 WG40907 12 3211 4 2 7/31/2012 Freshwater Drum m 7 38 718 L18333-7 8-Aug-12 WG40907 12 3212 4 2 7/31/2012 Freshwater Drum m 4 32 407 L18333-8 8-Aug-12 WG40907 12 3213 4 2 7/31/2012 Freshwater Drum m 5 33 476 L18333-9 8-Aug-12 WG40907 12 3214 4 2 7/31/2012 Smallmouth Bass m 3 26 288 L18333-10 8-Aug-12 WG40907 Perfluorochemicals in Mississippi River Pool 2 May 2013 44 Minnesota Pollution Control Agency MPCA ID Section Run Sample Date Species Length Sex Age (cm) Weight (g) AXYS ID Sample Received Work Group 12 3215 4 3 7/31/2012 Common Carp j 2 26 339 L18333-11 8-Aug-12 WG40908 12 3216 4 3 7/31/2012 Common Carp m 2 26 301 L18333-12 8-Aug-12 WG40908 12 3217 4 3 7/31/2012 Freshwater Drum m 8 40 888 L18333-13 8-Aug-12 WG40908 12 3218 4 3 7/31/2012 Smallmouth Bass f 3 26 236 L18333-14 8-Aug-12 WG40908 12 3219 4 3 7/31/2012 Smallmouth Bass m 5 33 628 L18333-15 8-Aug-12 WG40908 12 3220 4 3 7/31/2012 Smallmouth Bass j 2 17 62.4 L18333-16 8-Aug-12 WG40908 12 3221 4 3 7/31/2012 Smallmouth Bass j 2 16 65.04 L18333-17 8-Aug-12 WG40908 12 3222 4 3 7/31/2012 Smallmouth Bass j 3 23 198 L18333-18 8-Aug-12 WG40908 12 3223 4 3 7/31/2012 Smallmouth Bass m 2 21 168 L18333-19 8-Aug-12 WG40908 12 3224 4 3 7/31/2012 White Bass m 2 27 338 L18333-20 8-Aug-12 WG40908 12 3225 4 3 7/31/2012 White Bass j 1 19.6 123.88 L18333-21 8-Aug-12 WG40908 12 3226 4 4 7/31/2012 White Bass j 1 19.5 112.24 L18333-22 8-Aug-12 WG40908 12 3227 4 4 7/31/2012 White Bass j 1 18 99.32 L18333-23 8-Aug-12 WG40908 12 3228 4 4 7/31/2012 White Bass m 1 19 117.95 L18333-24 8-Aug-12 WG40908 12 3229 4 4 7/31/2012 White Bass m 5 35.5 705 L18333-25 (A) 8-Aug-12 WG40908 12 3230 4 4 7/31/2012 Bluegill Sunfish j 1 9 15.64 L18333-26 8-Aug-12 WG40908 12 3231 4 4 7/31/2012 Bluegill Sunfish j 1 9 16.21 L18333-27 8-Aug-12 WG40908 12 3232 4 4 7/31/2012 Bluegill Sunfish m 5 15 94.27 L18333-28 8-Aug-12 WG40908 12 3233 4 4 7/31/2012 Bluegill Sunfish m 5 15 99.2 L18333-29 8-Aug-12 WG40908 12 3237 4 4 7/31/2012 Bluegill Sunfish j 1 8 11.61 L18333-33 8-Aug-12 WG40909 12 3238 3 1 8/1/2012 White Bass m 6 37 815 L18332-1 7-Aug-12 WG40904 12 3239 3 1 8/1/2012 White Bass m 2 28 367 L18332-2 7-Aug-12 WG40904 12 3240 3 1 8/1/2012 Bluegill Sunfish f 6 17.5 137 L18332-3 7-Aug-12 WG40904 12 3241 3 1 8/1/2012 Bluegill Sunfish f 6 17.5 115 L18332-4 7-Aug-12 WG40904 12 3242 3 1 8/1/2012 Bluegill Sunfish m 6 17.5 144 L18332-5 7-Aug-12 WG40904 12 3243 3 1 8/1/2012 Bluegill Sunfish f 6 17.5 113 L18332-6 7-Aug-12 WG40904 12 3244 3 1 8/1/2012 Bluegill Sunfish f 7 18.5 274 L18332-7 7-Aug-12 WG40905 12 3245 3 1 8/1/2012 Bluegill Sunfish m 7 18.5 160.18 L18332-8 7-Aug-12 WG40905 Perfluorochemicals in Mississippi River Pool 2 May 2013 45 Minnesota Pollution Control Agency MPCA ID Section Run Sample Date Species Length Sex Age (cm) Weight (g) AXYS ID Sample Received Work Group 12 3246 3 1 8/1/2012 Bluegill Sunfish m 6 17 123.56 L18332-9 7-Aug-12 WG40905 12 3247 3 1 8/1/2012 Bluegill Sunfish m 5 15 93 L18332-10 7-Aug-12 WG40905 12 3248 3 1 8/1/2012 Bluegill Sunfish m 6 16.5 119 L18332-11 7-Aug-12 WG40905 12 3249 3 1 8/1/2012 Bluegill Sunfish m 6 17 116 L18332-12 7-Aug-12 WG40905 12 3250 3 1 8/1/2012 Bluegill Sunfish m 6 17.5 110 L18332-13 7-Aug-12 WG40905 12 3251 3 1 8/1/2012 Bluegill Sunfish f 4 14.5 66.36 L18332-14 7-Aug-12 WG40905 12 3252 3 1 8/1/2012 Bluegill Sunfish ind 8 19.5 173 L18332-15 7-Aug-12 WG40905 12 3253 3 1 8/1/2012 Bluegill Sunfish m 5 16.5 129 L18332-16 7-Aug-12 WG40905 12 3254 3 1 8/1/2012 Bluegill Sunfish f 6 17 121 L18332-17 7-Aug-12 WG40905 12 3255 2 2 8/1/2012 Common Carp m 6 51 2300 L18332-18 (A) 7-Aug-12 WG40905 12 3256 2 2 8/2/2012 Common Carp m 7 52 2500 L18332-19 7-Aug-12 WG40905 12 3257 2 2 8/2/2012 Common Carp m 3 33 528 L18332-20 7-Aug-12 WG40905 12 3258 2 2 8/2/2012 Common Carp j 3 31 508 L18332-21 7-Aug-12 WG40905 12 3259 2 2 8/2/2012 Common Carp j 2 30 414 L18332-22 7-Aug-12 WG40905 12 3260 2 2 8/2/2012 Common Carp m 3 35 646 L18332-23 7-Aug-12 WG40905 12 3261 2 2 8/2/2012 Bluegill Sunfish j 5 15 68 L18332-24 7-Aug-12 WG40905 12 3262 2 2 8/2/2012 Bluegill Sunfish j 2 10 35 L18332-25 7-Aug-12 WG40905 12 3263 2 2 8/2/2012 Bluegill Sunfish j 2 10 19 L18332-26 7-Aug-12 WG40906 12 3264 2 2 8/2/2012 Bluegill Sunfish j 1 8.5 12 L18332-27 7-Aug-12 WG40906 12 3265 2 2 8/2/2012 Bluegill Sunfish j 2 11 30 L18332-28 7-Aug-12 WG40906 12 3266 2 2 8/2/2012 Bluegill Sunfish j 1 9.5 19 L18332-29 7-Aug-12 WG40906 12 3267 2 2 8/2/2012 Freshwater Drum m 9 43 880 L18332-30 7-Aug-12 WG40906 12 3268 2 2 8/2/2012 Freshwater Drum m 5 33 506 L18332-31 7-Aug-12 WG40906 12 3269 2 2 8/2/2012 Freshwater Drum m 6 36 596 L18332-32 7-Aug-12 WG40906 12 3270 2 2 8/2/2012 Freshwater Drum m 6 37 603 L18332-33 7-Aug-12 WG40906 12 3271 2 2 8/2/2012 Freshwater Drum m 5 35 498 L18332-34 7-Aug-12 WG40906 12 3272 2 2 8/2/2012 Freshwater Drum m 4 32.5 422 L18332-35 7-Aug-12 WG40906 12 3273 2 2 8/2/2012 Smallmouth Bass j 2 15 43 L18332-36 7-Aug-12 WG40906 Perfluorochemicals in Mississippi River Pool 2 May 2013 46 Minnesota Pollution Control Agency MPCA ID Section Run Sample Date Species Length Sex Age (cm) Weight (g) AXYS ID Sample Received Work Group 12 3274 2 2 8/2/2012 Smallmouth Bass j 2 16 61 L18332-37 7-Aug-12 WG40906 12 3275 2 2 8/2/2012 Smallmouth Bass j 2 16.5 64 L18332-38 7-Aug-12 WG40906 12 3276 2 2 8/2/2012 Smallmouth Bass m 2 19.5 117 L18332-39 7-Aug-12 WG40906 12 3277 2 2 8/2/2012 Smallmouth Bass m 2 21 127 L18332-40 7-Aug-12 WG40906 12 3278 2 3 8/2/2012 Common Carp m 3 37 815 L18332-41 (A) 7-Aug-12 WG40906 12 3279 2 3 8/2/2012 Common Carp m 3 34 725 L18332-42 7-Aug-12 WG40906 12 3280 2 3 8/2/2012 Common Carp m 3 35 729 L18332-43 7-Aug-12 WG40906 12 3281 2 3 8/2/2012 Freshwater Drum m 5 33 452 L18332-44 7-Aug-12 WG40906 12 3282 2 3 8/2/2012 Smallmouth Bass m 2 22 157 L18332-45 7-Aug-12 WG40907 12 3283 2 3 8/2/2012 Bluegill Sunfish j 2 11 30.22 L18332-46 7-Aug-12 WG40907 12 3284 2 3 8/2/2012 Bluegill Sunfish f 4 14.5 56 L18332-47 7-Aug-12 WG40907 12 3285 2 3 8/2/2012 Bluegill Sunfish m 3 14 64 L18332-48 7-Aug-12 WG40907 12 3286 2 3 8/2/2012 Bluegill Sunfish f 5 16 105 L18332-49 7-Aug-12 WG40907 12 3287 2 3 8/2/2012 Bluegill Sunfish f 2 11 31 L18332-50 7-Aug-12 WG40907 12 3288 2 3 8/2/2012 Bluegill Sunfish m 4 15 74 L18332-51 7-Aug-12 WG40907 12 3289 2 3 8/2/2012 Bluegill Sunfish m 2 11 30.75 L18332-52 7-Aug-12 WG40907 12 3290 2 3 8/2/2012 Bluegill Sunfish m 6 17 124 L18332-53 7-Aug-12 WG40907 12 3291 2 4 8/2/2012 Freshwater Drum m 9 44 1106 L18334-1 (A) 7-Aug-12 WG40909 12 3292 2 4 8/2/2012 Freshwater Drum m 6 37 765 L18334-2 7-Aug-12 WG40909 12 3293 2 4 8/2/2012 Freshwater Drum m 10 46 1353 L18334-3 7-Aug-12 WG40909 12 3294 2 4 8/2/2012 Smallmouth Bass m 6 33.5 596 L18334-4 7-Aug-12 WG40909 12 3295 2 4 8/2/2012 Smallmouth Bass f 1 15 44.8 L18334-5 7-Aug-12 WG40909 12 3296 2 4 8/2/2012 Smallmouth Bass m 3 24 204 L18334-6 7-Aug-12 WG40909 12 3297 2 4 8/2/2012 Smallmouth Bass j 2 21 114 L18334-7 7-Aug-12 WG40909 12 3298 2 4 8/2/2012 Smallmouth Bass j 2 16 62 L18334-8 7-Aug-12 WG40909 12 3299 3 2 8/2/2012 Common Carp m 7 56.5 3200 L18334-9 7-Aug-12 WG41230 12 3300 3 2 8/2/2012 Common Carp m 7 55 3800 L18334-10 (A) 7-Aug-12 WG41230 12 3301 3 2 8/2/2012 Common Carp m 4 39.5 1184 L18334-11 7-Aug-12 WG41230 Perfluorochemicals in Mississippi River Pool 2 May 2013 47 Minnesota Pollution Control Agency MPCA ID Section Run Sample Date Species Length Sex Age (cm) Weight (g) AXYS ID Sample Received Work Group 12 3302 3 2 8/2/2012 Common Carp m 5 47 1722 L18334-12 7-Aug-12 WG41230 12 3303 3 2 8/2/2012 Common Carp f 4 43 1248 L18334-13 7-Aug-12 WG40910 12 3304 3 2 8/2/2012 Common Carp m 3 37.5 934 L18334-14 7-Aug-12 WG40909 12 3305 3 2 8/2/2012 Common Carp m 6 51.5 2500 L18334-15 7-Aug-12 WG41230 12 3306 3 2 8/2/2012 Common Carp m 7 52.5 2300 L18425-1 (A) 22-Aug-12 WG41077 12 3307 3 2 8/2/2012 Common Carp m 3 36.5 839 L18425-2 22-Aug-12 WG41077 12 3308 3 2 8/2/2012 Common Carp m 4 43 1617 L18425-3 22-Aug-12 WG41077 12 3309 3 2 8/2/2012 Common Carp j 4 41 1256 L18425-4 22-Aug-12 WG41077 12 3310 3 2 8/2/2012 Common Carp f 3 34.5 604 L18425-5 22-Aug-12 WG41077 12 3311 3 2 8/2/2012 Common Carp f 7 56 3000 L18425-6 22-Aug-12 WG41077 12 3312 3 2 8/2/2012 Common Carp f 7 56 3300 L18425-7 22-Aug-12 WG41077 12 3313 3 2 8/2/2012 Common Carp f 7 55.5 3200 L18425-8 22-Aug-12 WG41077 12 3314 3 2 8/2/2012 Freshwater Drum f 6 37.0 736 L18427-1 22-Aug-12 WG41078 12 3315 3 2 8/2/2012 Freshwater Drum m 6 36.0 1264 L18427-2 22-Aug-12 WG41078 12 3316 3 2 8/2/2012 White Bass m 5 36.5 849 L18427-3 22-Aug-12 WG41078 12 3317 3 2 8/2/2012 White Bass f 5 36.0 721 L18427-4 (A) 22-Aug-12 WG41078 12 3318 3 2 8/2/2012 White Bass f 3 31.0 566 L18427-5 22-Aug-12 WG41079 12 3319 3 2 8/2/2012 White Bass m 3 31.5 500 L18427-6 22-Aug-12 WG41079 12 3320 3 2 8/2/2012 White Bass f 3 31.5 592 L18427-7 22-Aug-12 WG41079 12 3321 3 2 8/2/2012 White Bass f 3 31.0 541 L18427-8 22-Aug-12 WG41079 12 3322 3 2 8/2/2012 White Bass m 2 28.0 379 L18427-9 22-Aug-12 WG41079 12 3323 3 2 8/2/2012 Smallmouth Bass j 2 16.5 65 L18427-10 22-Aug-12 WG41079 12 3324 3 2 8/2/2012 Smallmouth Bass j 2 16.0 69 L18427-11 22-Aug-12 WG41079 12 3325 3 2 8/2/2012 Smallmouth Bass j 2 22.0 168 L18427-12 22-Aug-12 WG41079 12 3326 3 2 8/2/2012 Smallmouth Bass f 6 35.5 698 L18427-13 22-Aug-12 WG41079 12 3327 3 3 8/2/2012 Freshwater Drum f 8 40.5 938 L18427-14 22-Aug-12 WG41079 12 3328 3 3 8/2/2012 Freshwater Drum f 5 33.0 494 L18427-15 22-Aug-12 WG41079 12 3329 3 3 8/2/2012 White Bass j 3 33.0 636 L18427-16 22-Aug-12 WG41079 Perfluorochemicals in Mississippi River Pool 2 May 2013 48 Minnesota Pollution Control Agency MPCA ID Section Run Sample Date Species Length Sex Age (cm) Weight (g) AXYS ID Sample Received Work Group 12 3330 3 3 8/2/2012 White Bass f 5 36.0 870 L18427-17 22-Aug-12 WG41079 12 3331 3 3 8/2/2012 White Bass f 6 37.5 985 L18427-18 22-Aug-12 WG41079 12 3332 3 3 8/2/2012 White Bass ind 4 33.0 605 L18427-19 22-Aug-12 WG41079 12 3333 3 3 8/2/2012 White Bass m 4 34.0 728 L18427-20 22-Aug-12 WG41079 12 3334 3 3 8/2/2012 White Bass f 4 35.0 780 L18427-21 (A) 22-Aug-12 WG41079 12 3335 3 3 8/2/2012 Smallmouth Bass j 1 15.5 52 L18427-22 22-Aug-12 WG41079 12 3336 3 3 8/2/2012 Smallmouth Bass j 1 14.0 48 L18427-23 22-Aug-12 WG41079 12 3337 3 3 8/2/2012 Smallmouth Bass j 2 24.5 267 L18427-24 22-Aug-12 WG41079 12 3338 1 6 8/3/2012 White Bass m 3 32.5 621 L18426-1 22-Aug-12 WG41077 12 3339 1 7 8/3/2012 White Bass j 4 34.0 582 L18426-2 22-Aug-12 WG41077 12 3340 1 7 8/3/2012 White Bass f 3 32.5 621 L18426-3 22-Aug-12 WG41077 12 3341 1 7 8/3/2012 White Bass f 4 34.0 619 L18426-4 22-Aug-12 WG41077 12 3342 1 7 8/3/2012 White Bass j 1 18.5 101 L18426-5 22-Aug-12 WG41077 12 3343 2 5 8/3/2012 Smallmouth Bass m 7 39.5 1121 L18426-6 22-Aug-12 WG41077 12 3344 2 5 8/3/2012 Smallmouth Bass m 4 30.0 514 L18426-7 22-Aug-12 WG41077 12 3345 2 5 8/3/2012 Smallmouth Bass j 1 20.5 129 L18426-8 22-Aug-12 WG41077 12 3346 2 5 8/3/2012 Smallmouth Bass j 0 13.5 41 L18426-9 22-Aug-12 WG41077 12 3347 3 5 8/7/2012 Freshwater Drum f 3 28.0 309 L18426-10 22-Aug-12 WG41077 12 3348 3 5 8/7/2012 Freshwater Drum m 4 31.5 407 L18426-11 22-Aug-12 WG41077 12 3349 3 5 8/7/2012 Freshwater Drum m 5 33.5 523 L18426-12 22-Aug-12 WG41077 12 3350 3 5 8/7/2012 Freshwater Drum m 5 33.5 397 L18426-13 22-Aug-12 WG41078 12 3351 3 5 8/7/2012 Freshwater Drum f 5 34.0 497 L18426-14 22-Aug-12 WG41078 12 3352 3 5 8/7/2012 Freshwater Drum j 3 26.5 260 L18426-15 22-Aug-12 WG41078 12 3353 3 6 8/7/2012 Freshwater Drum m 5 34.5 530 L18426-16 22-Aug-12 WG41078 12 3354 3 6 8/7/2012 Freshwater Drum f 11 48.0 1280 L18426-17 22-Aug-12 WG41078 12 3355 3 6 8/7/2012 Freshwater Drum f 7 37.5 654 L18426-18 22-Aug-12 WG41078 12 3356 3 5 8/7/2012 Freshwater Drum ind 5 34.0 432 L18426-19 22-Aug-12 WG41078 12 3357 3 5 8/7/2012 Freshwater Drum f 7 38.5 828 L18426-20 22-Aug-12 WG41078 Perfluorochemicals in Mississippi River Pool 2 May 2013 49 Minnesota Pollution Control Agency MPCA ID Section Run Sample Date Species Length Sex Age (cm) Weight (g) AXYS ID Sample Received Work Group 12 3358 3 4 8/6/2012 Smallmouth Bass ind 6 34.5 755 L18426-21 22-Aug-12 WG41078 12 3359 3 4 8/6/2012 Smallmouth Bass j 2 22.5 172 L18426-22 22-Aug-12 WG41078 12 3360 3 4 8/6/2012 Smallmouth Bass j 2 23.5 215 L18426-23 22-Aug-12 WG41078 12 3361 3 4 8/6/2012 Smallmouth Bass f 3 25.0 195 L18426-24 22-Aug-12 WG41078 12 3362 3 4 8/6/2012 Smallmouth Bass j 3 26.0 309 L18426-25 22-Aug-12 WG41078 12 3363 3 4 8/6/2012 Smallmouth Bass j 2 22.5 161 L18426-26 22-Aug-12 WG41078 12 3364 3 4 8/6/2012 Smallmouth Bass j 3 25.5 266 L18426-27 22-Aug-12 WG41078 12 3365 3 4 8/6/2012 Smallmouth Bass j 2 21.5 168 L18426-28 22-Aug-12 WG41078 12 3366 1 8 8/23/2012 White Bass M 2 27.5 357 L18746-1 25-Oct-12 WG41790 12 3367 1 9 8/23/2012 White Bass M 3 29.5 453 L18746-2 25-Oct-12 WG41790 12 3368 1 9 8/23/2012 White Bass F 3 29.0 457 L18746-3 (A) 25-Oct-12 WG41790 12 3369 1 10 9/14/2012 White Bass M 1 22.5 205 L18746-4 25-Oct-12 WG41790 12 3370 4 5 9/14/2012 Bluegill Sunfish F 5 16.5 126 L18746-5 25-Oct-12 WG41790 12 3371 4 5 9/14/2012 Bluegill Sunfish M 4 15.0 101 L18746-6 25-Oct-12 WG41790 12 3372 4 5 9/14/2012 Bluegill Sunfish M 4 14.5 79 L18746-7 25-Oct-12 WG41790 12 3373 4 6 9/14/2012 White Bass F 2 23.5 198 L18746-8 25-Oct-12 WG41790 12 3374 4 6 9/14/2012 White Bass M 2 27.5 403 L18746-9 25-Oct-12 WG41790 Perfluorochemicals in Mississippi River Pool 2 May 2013 50 Minnesota Pollution Control Agency Appendix A-6. Georeferences for fish-collection runs SECTION 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 RUN 1 1 2 2 3 3 4 4 5 5 6 6 7 7 8 9 9 10 10 SHOCKER START/STOP POINT START STOP START STOP START STOP START STOP START STOP START STOP START STOP START/STOP START STOP START STOP WAY POINT A11 A12 A13 A14 A15 A16 A17 A18 A19 A20 A21 A22 A23 A24 A25 A26 A27 A28 A29 DATE 7/30/2012 7/30/2012 7/30/2012 7/30/2012 7/30/2012 8/3/2012 8/3/2012 8/23/2012 8/23/2012 9/14/2012 2 1 START 2 1 STOP 2 2 START 2 2 STOP B11 7/30/2012 B12 B13 8/2/2012 B14 LAT 44.91298500 44.90704600 44.91334400 44.91065300 44.91300200 44.90414000 44.90265100 44.89440400 44.89791100 44.89823200 44.89339600 44.88920600 44.91494900 44.89812900 44.90580000 44.89131000 44.89001500 44.91268800 44.90779800 44.89816700 44.90448800 44.91427600 44.92089800 LONG -93.20236500 -93.19834200 -93.19959200 -93.20110100 -93.19911400 -93.19102000 -93.19044600 -93.16683400 -93.15615200 -93.15167100 -93.17746200 -93.17806300 -93.19989300 -93.15141900 -93.19552100 -93.17790600 -93.17793300 -93.20227600 -93.19938700 -93.14680100 -93.13751200 -93.13118100 -93.12492400 BLUEGILL 15 1 6 COMMON CARP 7 2 4 2 6 6 FRESHWATER DRUM 12 3 5 6 SMALLMOUTH BASS 11 3 1 5 WHITE BASS 1 2 1 1 4 1 2 1 15 Perfluorochemicals in Mississippi River Pool 2 May 2013 51 Minnesota Pollution Control Agency SECTION 2 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 3 3 3 3 4 4 4 4 RUN 3 3 4 4 5 5 1 1 2 2 3 3 4 4 5 5 6 6 7 7 1 1 2 2 SHOCKER START/STOP POINT START STOP START STOP START STOP START STOP START STOP START STOP START STOP START STOP START STOP START STOP START STOP START STOP WAY POINT B15 B16 B17 B18 B19 B20 C11 C12 C13 C14 C15 C16 C17 C18 C19 C20 C21 C22 C23 C24 D11 D12 D13 D14 DATE 8/2/2012 8/2/2012 8/3/2012 8/1/2012 8/2/2012 8/2/2012 8/6/2012 8/6/2012 8/7/2012 8/7/2012 7/31/2012 7/31/2012 LAT 44.92934000 44.92989700 44.90931400 44.89890800 44.90548900 44.91835700 44.83996700 44.83113900 44.89320800 44.89088900 44.87685700 44.87221900 44.84301000 44.83152500 44.86691200 44.83817400 44.88340400 44.87792600 44.89320800 44.89265500 44.78347500 44.78496000 44.78348100 44.78201300 LONG -93.11015700 -93.11013000 -93.04486800 -93.03396000 -93.13855700 -93.13107800 -93.00670700 -93.00342900 -93.02393300 -93.02063400 -93.01267000 -93.01331900 -93.00897500 -93.00890000 -93.01354400 -93.01379000 -93.01758800 -93.01650200 -93.02355800 -93.02391300 -92.93407500 -92.91887800 -92.89555300 -92.88821700 BLUEGILL 8 15 7 COMMON CARP 3 15 9 4 FRESHWATER DRUM 1 3 2 2 6 3 2 9 5 SMALLMOUTH BASS 1 5 4 4 3 8 8 1 WHITE BASS 2 7 6 5 Perfluorochemicals in Mississippi River Pool 2 May 2013 52 Minnesota Pollution Control Agency SECTION 4 4 4 4 4 4 4 4 RUN 3 3 4 4 5 5 6 6 SHOCKER START/STOP POINT START STOP START STOP START STOP START STOP WAY POINT D15 D16 D17 D18 D19 D20 D21 D22 DATE 7/31/2012 7/31/2012 9/14/2012 9/14/2012 LAT 44.77302400 44.77055200 44.76532300 44.76219200 44.78423600 44.78530800 44.78191000 44.78166100 LONG -92.91954700 -92.89832600 -92.89108600 -92.87201600 -92.93068700 -92.92324300 -92.92643200 -92.92239600 BLUEGILL 8 3 COMMON CARP 2 FRESHWATER DRUM 1 SMALLMOUTH BASS 6 WHITE BASS 2 4 2 Perfluorochemicals in Mississippi River Pool 2 May 2013 53 Minnesota Pollution Control Agency Appendix A-7. Georeferences for water sample stations Water Stations 1 2 3 4 5 6 7 8 9 10 11 12 Section 1 1 1 2 2 2 3 3 3 4 4 4 Description main channel at river mile 847 old Minnesota River channel, near river mile 845.4 main channel upstream of confluence with Minnesota River at river mile 844 main channel near boat launch at river mile 842 main channel adjacent to Holman Field at river mile 837 bay south of Pigs Eye Lake near river mile 834 main channel near boat launch and downstream from St. Paul Park Refinery at river mile 829.5 main channel upstream of Island 112 at river mile 827.5 Spring Lake, near river mile 821 main channel, downstream of Grey Cloud Slough at river mile 819 near shoreline, downstream of 3M Cottage Grove discharge, near river mile 817.5 main channel, upstream of Lock & Dam No. 2 at river mile 816 Lat(DD) 44.9103 44.8936 44.8974 44.9186 44.9317 44.8964 44.8395 44.8229 44.7653 44.7810 44.7823 44.7663 Long (DD) 93.2009 93.1765 93.1568 93.1300 93.0497 93.0266 93.0116 93.0092 92.9540 92.9324 92.8923 92.8731 Perfluorochemicals in Mississippi River Pool 2 May 2013 54 Minnesota Pollution Control Agency Appendix B: Benthic Macroinvertebrate and Sediment Data Appendix B-1. PFCs in Sediment samples from Pool 2 ............................................................................... 56 Appendix B-2. PFCs for Benthic Macroinvertebrate samples from Pool 2 ................................................. 58 Appendix B-3. Combined sediment and invertebrate PFOS concentrations, sediment organic carbon, and georeferences ......................................................................................................................... 61 Appendix B-4. QA results for sediment and invertebrate work groups ..................................................... 64 PFCs Mississippi River Pool 2 May 2013 55 Minnesota Pollution Control Agency Appendix B-1. PFCs in Sediment samples from Pool 2 CLIENT ID P2_837_SED MC_3_SED P2_833_R_SED P2_835_L_SED P2_835_R_SED P2_836_L_SED P2_836_R_SED P2_525_SED P2_829.5_SED P2_831_SED P2_832.5_R_SED P2_838_SED P2_832_SED P2_841_SED P2_842_SED P2_844_SED P2_846_SED P2_847_SED MC_1_SED MC_2_SED A SED S SED T SED F SED B SED C SED D SED E SED O SED P SED Q SED R SED WORK GROUP 40114 40114 40114 40114 40114 40114 40114 40114 40114 40114 40114 40114 40114 40114 40114 40114 40114 40114 40114 40114 41757 41757 41757 41757 41757 41757 41757 41757 41757 41757 41757 41757 Sample (g-dry) 5.00 5.05 4.97 5.10 5.13 5.01 5.16 5.02 4.96 5.23 5.02 5.32 5.16 5.26 5.03 5.21 5.27 5.05 4.98 5.25 5.16 5.15 5.03 5.09 5.14 4.98 4.99 5.00 5.01 5.15 5.72 5.06 PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA QA Mtuoreis Review (%) Date Qualified Analytes < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.200 < 0.200 < 0.200 < 0.100 25.4 7/24/2012 NONE < 0.0991 < 0.0991 < 0.0991 < 0.0991 < 0.0991 < 0.0991 < 0.0991 < 0.0991 < 0.0991 < 0.198 < 0.198 0.581 < 0.0991 26.8 7/24/2012 NONE < 0.101 < 0.101 < 0.101 < 0.101 < 0.101 0 . 1 1 3 0 . 1 2 6 < 0.101 < 0.101 < 0.201 < 0.201 0.681 < 0.101 57.2 7/24/2012 NONE < 0.0980 < 0.0980 < 0.0980 < 0.0980 < 0.0980 < 0.0980 < 0.0980 < 0.0980 < 0.0980 < 0.196 < 0.196 1 . 5 8 < 0.0980 40.9 7/24/2012 NONE < 0.0975 < 0.0975 < 0.0975 < 0.0975 < 0.0975 < 0.0975 < 0.0975 < 0.0975 < 0.0975 < 0.195 < 0.195 < 0.195 < 0.0975 33.2 7/24/2012 NONE < 0.0998 < 0.0998 < 0.0998 < 0.0998 < 0.0998 < 0.0998 < 0.0998 < 0.0998 < 0.0998 < 0.200 < 0.200 0.292 < 0.0998 53.0 7/24/2012 NONE < 0.0970 < 0.0970 < 0.0970 < 0.0970 < 0.0970 < 0.0970 < 0.0970 < 0.0970 < 0.0970 < 0.194 < 0.194 0 . 4 9 < 0.0970 55.1 7/24/2012 NONE < 0.0995 < 0.0995 < 0.0995 < 0.0995 < 0.0995 < 0.0995 0 . 1 0 1 < 0.0995 < 0.0995 < 0.199 < 0.199 0.525 < 0.0995 61.9 7/24/2012 NONE < 0.101 < 0.101 < 0.101 < 0.101 < 0.101 < 0.101 < 0.101 < 0.101 < 0.101 < 0.202 < 0.202 < 0.202 < 0.101 33.5 7/24/2012 NONE < 0.0956 < 0.0956 < 0.0956 < 0.0956 < 0.0956 < 0.0956 < 0.0956 < 0.0956 < 0.0956 < 0.191 < 0.191 0.195 < 0.0956 45.1 7/24/2012 NONE < 0.0996 < 0.0996 < 0.0996 < 0.0996 < 0.0996 < 0.0996 < 0.0996 < 0.0996 < 0.0996 < 0.199 < 0.199 0.594 < 0.0996 38.3 7/24/2012 NONE < 0.0941 < 0.0941 < 0.0941 < 0.0941 < 0.0941 < 0.0941 < 0.0941 < 0.0941 < 0.0941 < 0.188 < 0.188 < 0.188 < 0.0941 36.7 7/24/2012 NONE < 0.0969 < 0.0969 < 0.0969 < 0.0969 < 0.0969 < 0.0969 < 0.0969 < 0.0969 < 0.0969 < 0.194 < 0.194 0.299 < 0.0969 28.6 7/24/2012 NONE < 0.0950 < 0.0950 < 0.0950 < 0.0950 < 0.0950 < 0.0950 < 0.0950 < 0.0950 < 0.0950 < 0.190 < 0.190 0.252 < 0.0950 45.6 7/24/2012 NONE < 0.0995 < 0.0995 < 0.0995 < 0.0995 < 0.0995 < 0.0995 < 0.0995 < 0.0995 < 0.0995 < 0.199 < 0.199 0.222 < 0.0995 42.9 7/24/2012 NONE < 0.0959 < 0.0959 < 0.0959 < 0.0959 < 0.0959 < 0.0959 < 0.0959 < 0.0959 < 0.0959 < 0.192 < 0.192 < 0.192 < 0.0959 32.8 7/24/2012 NONE < 0.0948 < 0.0948 < 0.0948 < 0.0948 < 0.0948 < 0.0948 < 0.0948 < 0.0948 < 0.0948 < 0.190 < 0.190 < 0.190 < 0.0948 20.8 7/24/2012 NONE < 0.0990 < 0.0990 < 0.0990 < 0.0990 < 0.0990 < 0.0990 < 0.0990 < 0.0990 < 0.0990 < 0.198 < 0.198 < 0.198 < 0.0990 22.2 7/24/2012 NONE < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 0 . 1 9 8 0 . 1 2 4 < 0.201 < 0.201 0.905 0 . 2 8 44.6 7/24/2012 NONE < 0.0952 < 0.0952 < 0.0952 < 0.0952 < 0.0952 < 0.0952 < 0.0952 < 0.0952 < 0.0952 < 0.190 < 0.190 0.672 0 . 3 4 4 32.2 7/24/2012 NONE < 0.0969 < 0.0969 < 0.0969 < 0.0969 < 0.0969 < 0.0969 < 0.0969 < 0.0969 < 0.0969 < 0.194 < 0.194 0.299 < 0.0969 36.0 11/19/2012 NONE 0 . 1 4 8 < 0.0971 < 0.0971 < 0.0971 0 . 1 4 2 < 0.0971 0 . 1 0 8 < 0.0971 < 0.0971 < 0.194 < 0.194 0.943 < 0.0971 56.9 11/19/2012 NONE 0 . 1 7 6 < 0.0994 < 0.0994 < 0.0994 0 . 1 4 7 < 0.0994 0 . 1 0 1 < 0.0994 < 0.0994 < 0.199 < 0.199 1 . 1 0 < 0.0994 54.4 11/19/2012 NONE < 0.0983 < 0.0983 < 0.0983 < 0.0983 < 0.0983 < 0.0983 < 0.0983 < 0.0983 < 0.0983 < 0.197 < 0.197 0.282 < 0.0983 28.6 11/19/2012 NONE < 0.0973 < 0.0973 < 0.0973 < 0.0973 < 0.0973 < 0.0973 < 0.0973 < 0.0973 < 0.0973 < 0.195 < 0.195 0.566 < 0.0973 47.4 11/19/2012 NONE 0 . 1 2 4 < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.201 < 0.201 0.723 < 0.100 50.8 11/19/2012 NONE < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.200 < 0.200 0.473 < 0.100 42.7 11/19/2012 NONE 0 . 1 1 8 < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 0 . 1 0 5 < 0.100 < 0.100 < 0.200 < 0.200 0.586 < 0.100 52.6 11/19/2012 NONE < 0.0999 < 0.0999 < 0.0999 < 0.0999 < 0.0999 < 0.0999 < 0.0999 < 0.0999 < 0.0999 < 0.200 < 0.200 0.537 < 0.0999 30.4 11/19/2012 NONE < 0.0971 < 0.0971 < 0.0971 < 0.0971 < 0.0971 < 0.0971 < 0.0971 < 0.0971 < 0.0971 < 0.194 < 0.194 0.435 < 0.0971 26.3 11/19/2012 NONE 0 . 0 9 5 < 0.0874 < 0.0874 < 0.0874 < 0.0874 < 0.0874 < 0.0874 < 0.0874 < 0.0874 < 0.175 < 0.175 0.647 < 0.0874 33.8 11/19/2012 NONE 0 . 1 1 9 < 0.0987 < 0.0987 < 0.0987 0 . 1 8 0 < 0.0987 0 . 1 3 1 < 0.0987 < 0.0987 < 0.197 < 0.197 2 . 1 0 < 0.0987 44.9 11/19/2012 NONE Perfluorochemicals in Mississippi River Pool 2 May 2013 56 Minnesota Pollution Control Agency CLIENT ID G SED G SED FD H SED I SED J SED K SED K SED FD L SED M SED M SED FD N SED 517 SED1 517 SED2 517 SED3 518 SED 519 SED 520 SED 521 SED 522 SED 523 SED 524 SED 525 SED 526 SED 530 SED 532 SED 533 SED WORK GROUP 41758 41758 41758 41758 41758 41758 41758 41758 41758 41758 41758 38714 38714 38714 38714 38714 38714 38714 38714 38714 38714 38714 38714 38714 38714 38714 Sample (g-dry) 5.34 5.09 5.10 5.14 5.33 5.01 4.64 5.09 4.98 5.14 5.18 5.3 5.38 5.19 5.22 5.3 5.52 5.4 5.44 5.06 5.56 5.48 5.63 5.38 5.45 5.33 PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA QA Mtuoreis Review (%) Date Qualified Analytes < 0.0937 < 0.0937 < 0.0937 < 0.0937 < 0.0937 < 0.0937 < 0.0937 < 0.0937 < 0.0937 < 0.187 < 0.187 0.601 < 0.0937 40.6 12/3/2012 NONE < 0.0983 < 0.0983 < 0.0983 < 0.0983 < 0.0983 < 0.0983 < 0.0983 < 0.0983 < 0.0983 < 0.197 < 0.197 0 . 8 6 < 0.0983 45.9 12/3/2012 NONE < 0.0981 < 0.0981 < 0.0981 < 0.0981 < 0.0981 < 0.0981 < 0.0981 < 0.0981 < 0.0981 < 0.196 < 0.196 0.504 < 0.0981 42.8 12/3/2012 NONE 0 . 1 0 2 < 0.0972 < 0.0972 < 0.0972 < 0.0972 < 0.0972 < 0.0972 < 0.0972 < 0.0972 < 0.194 < 0.194 0.825 < 0.0972 36.3 12/3/2012 NONE 2 . 7 2 0 0 . 5 0 6 0 . 2 6 3 < 0.0938 0 . 0 9 7 < 0.0938 < 0.0938 < 0.0938 < 0.0938 < 0.188 < 0.188 0.335 < 0.0938 29.6 12/3/2012 PFHxA, PFOA 0 . 7 0 2 < 0.0997 < 0.0997 < 0.0997 0 . 7 1 9 < 0.0997 0 . 1 7 8 < 0.0997 < 0.0997 < 0.199 < 0.199 8 . 5 8 0 . 3 1 9 45.8 12/3/2012 PFDA, PFOA 0 . 6 7 2 < 0.108 < 0.108 < 0.108 0 . 5 9 4 < 0.108 0 . 1 3 4 < 0.108 < 0.108 < 0.216 < 0.216 6 . 3 8 0 . 3 2 3 43.5 12/3/2012 PFDA, PFOA < 0.0981 < 0.0981 < 0.0981 < 0.0981 < 0.0981 < 0.0981 < 0.0981 < 0.0981 < 0.0981 < 0.196 < 0.196 < 0.196 < 0.0981 25.2 12/3/2012 NONE < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.100 < 0.201 < 0.201 0.203 < 0.100 25.9 12/3/2012 NONE < 0.0973 < 0.0973 < 0.0973 < 0.0973 < 0.0973 < 0.0973 < 0.0973 < 0.0973 < 0.0973 < 0.195 < 0.195 0.366 < 0.0973 28.6 12/3/2012 NONE 0 . 2 5 2 < 0.0965 < 0.0965 < 0.0965 0 . 0 9 9 < 0.0965 < 0.0965 < 0.0965 < 0.0965 < 0.193 < 0.193 1 . 0 2 < 0.0965 30.6 12/3/2012 PFOA 1 . 9 5 0 0 . 2 2 9 0 . 2 8 7 0 . 1 5 2 3 . 9 2 0 0 . 1 3 6 0 . 5 5 3 0 . 1 3 4 0 . 3 2 9 0 . 4 5 5 0 . 6 4 6 80.20 2 . 5 1 59.3 2/8/2012 PFDoA 1 . 1 7 0 0 . 1 5 4 0 . 1 8 5 0 . 1 1 2 3 . 0 1 0 . 0 9 6 0 . 3 6 5 < 0.0930 0 . 2 4 7 0 . 2 9 8 0 . 4 2 8 33.30 1 . 3 9 51.1 2/8/2012 PFDoA 1 . 3 6 0 0 . 2 5 3 0 . 3 0 1 0 . 1 4 9 5 . 4 6 0 0 . 1 2 4 0 . 3 8 0 < 0.0964 0 . 2 2 4 0 . 3 2 9 0 . 6 3 2 41.10 1 . 6 7 52.9 2/8/2012 PFDoA < 0.0959 < 0.0959 < 0.0959 < 0.0959 < 0.0959 < 0.0959 < 0.0959 < 0.0959 < 0.0959 < 0.192 < 0.192 0.655 < 0.0959 4 2 2/8/2012 NONE < 0.0943 < 0.0943 < 0.0943 < 0.0943 < 0.0943 < 0.0943 < 0.0943 < 0.0943 < 0.0943 < 0.189 < 0.189 < 0.189 < 0.0943 23.7 2/8/2012 NONE < 0.0906 < 0.0906 < 0.0906 < 0.0906 < 0.0906 < 0.0906 < 0.0906 < 0.0906 < 0.0906 < 0.181 < 0.181 0.309 < 0.0906 31.1 2/8/2012 NONE < 0.0926 < 0.0926 < 0.0926 < 0.0926 < 0.0926 < 0.0926 < 0.0926 < 0.0926 < 0.0926 < 0.185 < 0.185 0.280 < 0.0926 32.3 2/8/2012 NONE < 0.0920 < 0.0920 < 0.0920 < 0.0920 < 0.0920 < 0.0920 < 0.0920 < 0.0920 < 0.0920 < 0.184 < 0.184 0.288 < 0.0920 32.1 2/8/2012 NONE < 0.0988 < 0.0988 < 0.0988 < 0.0988 < 0.0988 < 0.0988 < 0.0988 < 0.0988 < 0.0988 < 0.198 < 0.198 0.290 < 0.0988 36.7 2/8/2012 NONE < 0.0900 < 0.0900 < 0.0900 < 0.0900 < 0.0900 < 0.0900 < 0.0900 < 0.0900 < 0.0900 < 0.180 < 0.180 0.257 < 0.0900 25.9 2/8/2012 NONE 0 . 1 0 3 < 0.0912 < 0.0912 < 0.0912 < 0.0912 < 0.0912 < 0.0912 < 0.0912 < 0.0912 < 0.182 < 0.182 0.326 < 0.0912 39.2 2/8/2012 NONE 0 . 1 2 7 < 0.0888 < 0.0888 < 0.0888 0 . 0 9 2 < 0.0888 < 0.0888 < 0.0888 < 0.0888 < 0.178 < 0.178 0.713 < 0.0888 33.8 2/8/2012 NONE < 0.0930 < 0.0930 < 0.0930 < 0.0930 < 0.0930 < 0.0930 < 0.0930 < 0.0930 < 0.0930 < 0.186 < 0.186 0.882 < 0.0930 36.3 2/8/2012 NONE < 0.0918 < 0.0918 < 0.0918 < 0.0918 < 0.0918 < 0.0918 0 . 1 9 0 < 0.0918 < 0.0918 < 0.184 < 0.184 2.070 < 0.0918 45.6 2/8/2012 NONE < 0.0938 < 0.0938 < 0.0938 < 0.0938 < 0.0938 < 0.0938 < 0.0938 < 0.0938 < 0.0938 < 0.188 < 0.188 0.303 < 0.0938 40.7 2/8/2012 NONE Perfluorochemicals in Mississippi River Pool 2 May 2013 57 Minnesota Pollution Control Agency Appendix B-2. PFCs for Benthic Macroinvertebrate samples from Pool 2 CLIENT ID J INV M INV A INV WORK GROUP 41820 41820 41756 Sample (g-wet) 0.26 0.13 2.02 PFBA 2.54 < 3.85 < 0.495 PFPeA < 1.92 < 3.85 < 0.495 PFHxA < 1.92 < 3.85 < 0.495 PFHpA < 1.92 < 3.85 < 0.495 PFOA < 1.92 < 3.85 0.65 B INV C INV D INV 41756 41756 41756 1.00 < 1.00 < 1.00 < 1.00 < 1.00 2.01 < 0.498 < 0.498 < 0.498 < 0.536 < 1.00 0.976 2.01 < 0.498 < 1.22 < 0.797 < 0.498 < 0.498 PFNA < 1.92 < 3.85 1.53 PFDA < 1.92 < 3.85 0.522 PFUnA < 1.92 < 3.85 1.26 PFDoA < 1.92 < 3.85 < 0.495 PFBS < 3.85 < 7.69 < 0.990 PFHxS < 3.85 < 7.69 < 0.990 < 1.00 2.05 < 1.00 0.726 < 1.00 1.61 < 1.00 < 0.498 < 2.00 < 0.995 < 2.00 < 0.995 1.07 < 0.498 < 0.498 < 0.498 < 0.995 < 0.995 PFOS 31.6 35.4 9.28 PFOSA < 2.69 < 5.38 < 0.594 QA Review Date 12/27/2012 12/27/2012 12/27/2012 Qualified Analytes NONE NONE PFNA, PFDA, PFUnA, PFDoA, PFOSA 8.02 < 1.20 12/27/2012 NONE 15.8 < 0.597 12/27/2012 PFDOA, PFOS, PFOSA, PFUnA 8.6 < 0.597 12/27/2012 PFBA, PFDA, PFUnA, PFDoA, PFBS, PFHxS, PFOS, PFOSA E INV F INV G INV I INV K INV N INV O INV Q INV R INV 41756 41756 41756 41756 41756 41756 41756 41756 41756 1.41 < 0.709 < 0.709 < 0.709 < 0.709 0.944 1.37 < 0.709 0.96 < 0.709 < 1.42 < 1.42 11.9 < 0.851 12/27/2012 PFBA, PFDoA, PFOS, PFOSA, PFUnA 0.96 < 1.04 < 1.04 < 1.04 < 1.04 1.30 < 0.769 < 0.769 < 0.769 < 0.769 < 1.04 1.03 < 1.04 2.45 < 1.04 1.07 1.1 < 1.04 1.61 < 0.769 < 2.08 < 1.54 < 2.08 < 1.54 0.60 < 1.67 < 1.67 < 1.67 < 1.67 < 1.67 < 1.67 < 1.67 < 1.67 < 1.67 < 3.33 < 3.33 0.96 1.84 < 1.04 < 1.04 < 1.04 6.1 < 1.04 1.33 < 1.04 < 1.04 < 2.08 3.47 1.85 < 0.541 < 0.541 < 0.541 < 0.541 0.47 < 2.13 < 2.13 < 2.13 < 2.13 2.03 < 0.493 2.76 < 0.493 < 0.493 1.21 < 2.13 0.87 0.976 < 2.13 1.48 < 0.541 < 2.13 0.604 < 0.541 < 2.13 0.724 < 0.541 < 2.13 < 0.493 < 1.08 < 4.26 < 0.985 < 1.08 < 4.26 < 0.985 1.31 < 0.763 1.03 < 0.763 < 0.763 1.75 1.93 < 0.763 1.02 < 0.763 < 1.53 < 1.53 12.8 < 1.25 12/27/2012 PFDoA, PFUnA 23.1 < 0.923 12/27/2012 PFNA, PFDoA, PFUnA 18.8 < 2.00 12/27/2012 NONE 194 4.18 12/27/2012 PFBA, PFDoA, PFOS, PFOSA 37.2 < 0.649 12/27/2012 PFNA 23.7 < 2.55 12/27/2012 PFDoA 14.2 < 0.591 12/27/2012 PFPeA, PFDoA, PFBS, PFHxS, PFOS, PFOSA, PFUnA 32.2 < 0.916 12/27/2012 PFPea, PFBA, PFDoA, PFBS, PFHxS, PFOS, PFOSA, PFUnA Perfluorochemicals in Mississippi River Pool 2 May 2013 58 Minnesota Pollution Control Agency CLIENT ID S INV T INV P2_838_INV P2_836_R_INV P2_525_INV P2_831_INV P2_832.5_R_INV P2_832_INV P2_841_INV P2_842_INV P2_844_INV MC_1_INV P2_833_R_INV P2_835_L_INV P2_835_R_INV P2_836_L_INV 517 INV 526 INV 527 INV 530 INV 532 INV 533 INV 518 INV 519 INV 520 INV 521 INV 522 INV 523 INV WORK GROUP 41756 41756 40110 40110 40110 40110 40110 40110 40110 40110 40110 40110 40110 40110 40110 40110 38738 38738 38738 38738 38738 38738 38738 38738 38738 38738 38738 38738 Sample (g-wet) 1.54 1.73 0.46 0.38 0.36 0.68 0.46 0.53 0.30 0.68 0.33 0.69 0.38 0.24 0.75 0.71 1.98 2.04 0.68 2.07 1.99 2.04 2.04 2.00 2.05 2.03 1.01 1.98 PFBA < 0.649 < 0.578 < 1.09 2.8 < 1.39 < 0.735 < 1.09 < 0.943 < 0.735 < 1.52 < 0.725 < 1.32 < 2.08 < 0.667 < 0.704 6.10 < 0.245 < 0.735 < 0.242 < 0.251 < 0.245 < 0.245 < 0.250 < 0.244 < 0.332 < 0.495 < 0.253 PFPeA < 0.780 < 1.01 < 1.09 < 1.32 < 1.39 < 0.735 < 1.09 < 0.943 < 1.67 < 0.735 < 1.52 < 0.725 < 1.32 < 2.08 < 0.667 < 0.704 D 0.847 < 0.245 < 0.735 < 0.242 < 0.251 < 0.245 < 0.245 < 0.250 < 0.244 0.258 < 0.495 1.53 PFHxA < 0.729 < 0.578 < 1.09 < 1.32 < 1.39 < 0.735 < 1.09 < 0.943 < 1.67 < 0.735 < 1.52 < 0.725 < 1.32 < 2.08 < 0.667 < 0.704 < 0.758 < 0.245 < 0.735 < 0.242 0.327 0.287 < 0.245 < 0.250 < 0.244 0.562 0.678 < 0.253 PFHpA < 0.649 0.872 < 1.09 < 1.32 < 1.39 < 0.735 < 1.09 < 0.943 < 1.67 < 0.735 < 1.52 < 0.725 < 1.32 < 2.08 < 0.667 < 0.704 < 0.758 < 0.245 < 0.735 < 0.242 < 0.251 < 0.245 < 0.245 < 0.250 < 0.244 < 0.246 < 0.495 0.272 PFOA 1.81 1.11 < 1.09 4.3 < 1.39 < 0.735 < 1.09 < 0.943 < 1.67 < 0.735 < 1.52 < 0.725 < 1.32 < 2.08 < 0.667 0.743 13.6 < 0.245 < 0.735 < 0.242 < 0.251 < 0.245 < 0.245 < 0.250 < 0.244 0.36 < 0.495 0.529 PFNA 2.23 1.33 < 1.09 1.53 < 1.39 < 0.735 < 1.09 < 0.943 < 1.67 < 0.735 < 1.52 < 0.725 < 1.32 < 2.08 < 1.33 < 0.704 0.366 < 0.245 < 0.735 < 0.242 < 0.251 < 0.245 1.07 < 0.250 0.646 1.29 < 0.495 1.41 PFDA < 0.649 < 0.578 < 1.09 < 1.32 < 1.39 < 0.735 < 1.09 < 0.943 < 1.67 < 0.735 < 1.52 < 0.725 < 1.32 < 2.08 < 0.667 < 0.704 1.83 < 0.245 < 0.735 < 0.242 0.435 < 0.245 0.775 < 0.250 0.279 0.698 < 0.495 0.713 PFUnA < 0.649 < 0.578 < 1.09 < 1.32 < 1.39 < 0.735 < 1.09 < 0.943 < 1.67 < 0.735 < 1.52 < 0.725 < 1.32 < 2.08 < 0.667 < 0.704 1.45 < 0.245 < 0.735 < 0.242 0.503 < 0.245 0.533 < 0.250 < 0.244 0.386 < 0.495 0.697 PFDoA < 0.649 < 0.578 < 1.09 < 1.32 < 1.39 < 0.735 < 1.09 < 0.943 < 1.67 < 0.735 < 1.52 < 0.725 < 1.32 < 2.08 < 0.667 < 0.704 D 0.956 < 0.245 < 0.735 < 0.242 < 0.251 < 0.735 < 0.735 < 0.250 < 0.244 < 0.739 < 0.495 < 0.758 PFBS < 1.30 < 1.16 < 2.17 < 2.63 < 2.78 < 1.47 < 2.17 < 1.89 < 3.33 < 1.47 < 3.03 < 1.45 < 2.63 < 4.17 < 1.33 < 1.41 < 0.505 < 0.490 < 1.47 < 0.483 < 0.503 < 0.490 < 0.490 < 1.50 < 0.488 < 0.493 < 0.990 < 0.505 PFHxS < 1.30 < 1.16 < 5.43 < 6.58 < 6.94 < 3.68 < 5.43 < 4.72 < 8.33 < 3.68 < 7.58 < 3.62 < 6.58 < 10.4 < 3.33 < 3.52 6.28 < 0.490 < 1.47 < 0.483 < 0.503 < 0.490 < 0.490 < 1.50 < 0.488 < 0.493 < 0.990 < 0.505 PFOS 17.5 12.5 8.09 23.8 9.89 < 1.47 8.11 6.15 < 3.33 3.69 < 3.03 1.65 6.83 < 4.17 18.5 7.3 D 684 D 6.84 D 24.9 D 3.95 D 11.1 D 4.56 D 20.0 D 3.85 D 8.44 D 10.4 D 11.1 D 13.7 PFOSA < 0.779 < 0.694 < 1.52 < 1.84 < 1.94 < 1.03 < 1.52 < 1.32 < 2.33 < 1.03 < 2.12 1.76 < 1.84 < 2.92 1.42 < 0.986 D 18.3 < 0.343 < 1.03 0.622 < 0.352 < 0.343 < 1.03 < 0.350 < 0.341 < 0.345 < 0.693 < 1.06 QA Review Date 12/27/2012 12/27/2012 7/30/2012 7/30/2012 7/30/2012 7/30/2012 7/30/2012 7/30/2012 7/30/2012 7/30/2012 7/30/2012 7/30/2012 7/30/2012 7/30/2012 7/30/2012 7/30/2012 2/24/2012 2/24/2012 2/24/2012 2/24/2012 2/24/2012 2/24/2012 2/24/2012 2/24/2012 2/24/2012 2/24/2012 2/24/2012 2/24/2012 Qualified Analytes PFBA, PFPeA, PFHzA, PFHpA, PFNA, PFDA, PFUnA, PFDoA, PFBS, PFHxS, PFOS, PFOSA PFBA, PFPeA, PFHxA, PFHpA, PFNA, PFDA, PFUnA, PFDoA, PFBS, PFHxS, PFOS, PFOSA PFDoA, PFOSA PFOS, PFNA, PFDoA PFDoA, PFOSA PFDoA, PFOSA PFDoA, PFOSA PFDoA, PFOSA PFDoA, PFOSA PFDoA, PFOSA PFDoA, PFOSA PFDoA PFDoA, PFOSA PFDoA, PFOSA PFDoA PFOS, PFDoA, PFOSA PFNA NONE NONE NONE NONE NONE PFNA NONE PFNA NONE NONE PFNA, PFPeA, PFHpA Perfluorochemicals in Mississippi River Pool 2 May 2013 59 Minnesota Pollution Control Agency CLIENT ID 524 INV 525 INV WORK GROUP 38738 38738 Sample (g-wet) 2.01 1.99 PFBA < 0.249 < 0.251 PFPeA < 0.249 D 1.18 PFHxA < 0.249 < 0.754 PFHpA < 0.249 < 0.754 PFOA < 0.249 0.435 PFNA < 0.249 1.22 PFDA < 0.249 0.585 PFUnA < 0.249 0.752 PFDoA < 0.249 < 0.754 PFBS < 0.498 < 0.503 PFHxS < 0.498 < 0.503 PFOS < 1.49 D 14.3 PFOSA < 0.348 < 0.352 QA Review Date 2/24/2012 2/24/2012 Qualified Analytes NONE PFNA, PFPeA Perfluorochemicals in Mississippi River Pool 2 May 2013 60 Minnesota Pollution Control Agency Appendix B-3. Combined sediment and invertebrate PFOS concentrations, sediment organic carbon, and georeferences Site A B C D E F G G-FD H I J K K-FD L M M-FD N O P Q R S T P2_525 P2_829.5 P2_831 P2_832.5 P2_832 LAT (DD) 44.76008 44.75987 44.76015 44.75560 44.75565 44.77228 44.77130 44.77130 44.77388 44.78248 44.78188 44.78153 44.78153 44.77753 44.77775 44.77775 44.77783 44.76930 44.77142 44.76315 44.76607 44.75747 44.75977 44.82145 44.84305 44.86515 44.88443 44.87737 LONG (DD) -92.97092 -92.95783 -92.94590 -92.96452 -92.95207 -92.94797 -92.93517 -92.93517 -92.92642 -92.91725 -92.90643 -92.89293 -92.89293 -92.91138 -92.90055 -92.90055 -92.88902 -92.89307 -92.88312 -92.88422 -92.87648 -92.87737 -92.87493 -93.00985 -93.01288 -93.01217 -93.01708 -93.01652 Date Collected 10/10/12 15:00 10/10/12 14:40 10/10/12 14:45 10/10/12 15:25 10/10/12 15:45 10/16/12 10:40 10/16/12 11:20 10/16/12 11:25 10/16/12 11:42 10/16/12 11:36 10/16/12 13:10 10/16/12 15:06 10/16/12 15:10 10/16/12 13:25 10/16/12 13:55 10/16/12 13:58 10/16/12 14:16 10/10/12 13:11 10/10/12 13:47 10/10/12 12:45 10/10/12 12:05 10/10/12 11:00 10/10/12 11:40 05/23/12 13:55 05/23/12 14:30 05/23/12 13:05 05/23/12 11:20 05/23/12 10:45 Sediment Sample ID A SED B SED C SED D SED E SED F SED G SED G SED FD H SED I SED J SED K SED K SED FD L SED M SED M SED FD N SED O SED P SED Q SED R SED S SED T SED P2_525_SED P2_829.5_SED P2_831_SED P2_832.5_R_SED P2_832_SED Sediment PFOS (ng/g- dw) 0.299 0.566 0.723 0.473 0.586 0.282 0.601 0.86 0.504 0.825 0.335 8.58 6.38 < 0.196 0.203 0.366 1.02 0.537 0.435 0.647 2.1 0.943 1.1 0.525 < 0.202 0.195 0.594 0.299 Sediment Moisture (%) 36.0 47.4 50.8 42.7 52.6 28.6 40.6 45.9 42.8 36.3 29.6 45.8 43.5 25.2 25.9 28.6 30.6 30.4 26.3 33.8 44.9 56.9 54.4 61.9 33.5 45.1 38.3 28.6 Sediment PFOS (ng/gww) 0.191 0.298 0.356 0.271 0.278 0.201 0.357 0.465 0.288 0.526 0.236 4.650 3.605 0.150 0.261 0.708 0.374 0.321 0.428 1.157 0.406 0.502 0.200 0.107 0.366 0.213 Fraction Organic Carbon (foc) 0.97% 2.34% 1.91% 1.63% 1.80% 0.48% 0.95% 1.13% 1.06% 0.61% 0.62% 0.98% 0.70% 0.51% 0.36% 0.66% 0.63% 0.73% 0.55% 0.83% 0.99% 1.38% 1.19% 2.03% 0.80% 0.95% 0.99% 1.36% Sediment PFOS (ng/g OC) 30.7 24.2 38.0 29.0 32.6 59.2 63.0 76.1 47.8 135.6 54.2 874.2 916.7 56.2 55.8 161.8 73.9 78.7 77.7 212.2 68.3 92.4 25.9 20.5 59.9 22.0 Invertebrate Sample ID A INV B INV C INV D INV E INV F INV G INV I INV J INV K INV M INV N INV O INV Q INV R INV S INV T INV P2_525_INV P2_831_INV P2_832.5_R_INV P2_832_INV Invertebrate PFOS (ng/g- ww) 9.28 8.02 15.8 8.6 11.9 12.8 23.1 18.8 31.6 194 35.4 37.2 23.7 14.2 32.2 17.5 12.5 9.89 < 1.47 8.11 6.15 Perfluorochemicals in Mississippi River Pool 2 May 2013 61 Minnesota Pollution Control Agency Site MC_1 MC_2 MC_3 P2_833_R P2_835_L P2_835_R P2_836_L P2_836_R P2_837_R P2_838 P2_841 P2_842 P2_844 P2_846 P2_847 517 SED1 517 SED2 517 SED3 518 SED 519 SED 520 SED 521 SED 522 SED 523 SED 524 SED 525 SED 526 SED 530 SED 532 SED LAT (DD) 44.91560 44.91560 44.91560 44.88912 44.90982 44.91002 44.92053 44.92035 44.92900 44.93913 44.92575 44.91890 44.89698 44.89567 44.91193 44.78210 44.78210 44.78210 44.77012 44.76645 44.77222 44.77407 44.78468 44.79105 44.81015 44.82175 44.83353 44.89130 44.90618 LONG (DD) -93.04452 -93.04452 -93.04452 -93.01937 -93.04267 -93.04515 -93.05037 -93.05315 -93.05142 -93.05597 -93.11460 -93.12847 -93.15080 -93.18923 -93.20093 -92.88855 -92.88855 -92.88855 -92.94168 -92.95885 -92.96918 -93.01647 -93.01883 -93.02527 -93.00707 -93.00955 -93.01233 -93.01835 -93.04245 Date Collected 05/22/12 14:00 05/22/12 14:05 05/22/12 14:15 05/22/12 15:05 05/22/12 12:55 05/22/12 11:55 05/22/12 11:13 05/22/12 10:30 05/16/12 16:40 05/16/12 16:00 05/16/12 15:05 05/16/12 14:20 05/16/12 13:10 05/16/12 11:48 05/16/12 11:19 11/23/11 15:00 11/23/11 15:10 11/23/11 15:18 11/23/11 13:00 11/23/11 13:30 11/23/11 12:30 11/30/11 15:45 11/18/11 15:30 11/18/11 14:00 11/30/11 14:30 11/30/11 13:10 11/30/11 12:00 11/28/11 12:30 11/28/11 14:00 Sediment Sample ID MC_1_SED MC_2_SED MC_3_SED P2_833_R_SED P2_835_L_SED P2_835_R_SED P2_836_L_SED P2_836_R_SED P2_837_R_SED P2_838_SED P2_841_SED P2_842_SED P2_844_SED P2_846_SED P2_847_SED 517 SED1 517 SED2 517 SED3 518 SED 519 SED 520 SED 521 SED 522 SED 523 SED 524 SED 525 SED 526 SED 530 SED 532 SED Sediment PFOS (ng/g- dw) 0.905 0.672 0.581 0.681 1.58 < 0.195 0.292 0.49 < 0.200 < 0.188 0.252 0.222 < 0.192 < 0.190 < 0.198 80.20 33.30 41.10 0.66 0.09 0.31 0.28 0.29 0.29 0.26 0.33 0.71 0.88 2.07 Sediment Moisture (%) 44.6 32.2 26.8 57.2 40.9 33.2 53 55.1 25.4 36.7 45.6 42.9 32.8 20.8 22.2 59.3 51.1 52.9 42.0 23.7 31.1 32.3 32.1 36.7 25.9 39.2 33.8 36.3 45.6 Sediment PFOS (ng/gww) 0.501 0.456 0.425 0.291 0.934 0.137 0.220 0.137 0.127 32.641 16.284 19.358 0.380 0.072 0.213 0.190 0.196 0.184 0.190 0.198 0.472 0.562 1.126 Fraction Organic Carbon (foc) 2.17% 1.67% 0.59% 1.92% 1.32% 0.58% 12.75% 1.61% 0.14% 1.33% 2.52% 1.54% 0.79% 0.05% 0.06% 2.84% 1.75% 1.62% 1.17% 0.16% 0.63% 1.02% 0.64% 1.16% 0.25% 1.17% 0.95% 0.89% 1.52% Sediment PFOS (ng/g OC) 41.8 40.2 98.6 35.5 119.7 2.3 30.4 10.0 14.4 2823.9 1902.9 2537.0 56.0 57.6 48.8 27.5 44.7 25.0 104.0 27.9 74.7 98.7 136.2 Invertebrate Sample ID MC_1_INV P2_833_R_INV P2_835_L_INV P2_835_R_INV P2_836_L_INV P2_836_R_INV P2_838_INV P2_841_INV P2_842_INV P2_844_INV 517 INV 518 INV 519 INV 520 INV 521 INV 522 INV 523 INV 524 INV 525 INV 526 INV 530 INV 532 INV Invertebrate PFOS (ng/g- ww) 1.65 6.83 < 4.17 18.5 7.3 23.8 8.09 < 3.33 3.69 < 3.03 684 20 3.85 8.44 10.4 11.1 13.7 < 1.49 14.3 6.84 3.95 11.1 Perfluorochemicals in Mississippi River Pool 2 May 2013 62 Minnesota Pollution Control Agency Site 533 SED LAT (DD) 44.92512 LONG (DD) -93.05007 Date Collected 11/28/11 15:30 Sediment Sample ID 533 SED Sediment PFOS (ng/g- dw) 0.30 Sediment Moisture (%) 40.7 Sediment PFOS (ng/gww) 0.180 Fraction Organic Carbon (foc) 1.33% Sediment PFOS (ng/g OC) 22.8 Invertebrate Sample ID 533 INV Invertebrate PFOS (ng/g- ww) 4.56 Perfluorochemicals in Mississippi River Pool 2 May 2013 63 Minnesota Pollution Control Agency Appendix B-4. QA results for sediment and invertebrate work groups WORK GROUP 40114 CLIENT ID Lab Blank 40114 Spiked Matrix 40114 40114 41757 P2_846_SED (MS) P2_846_SED (MSD) Lab Blank 41757 Spiked Matrix 41757 F SED (MS) 41757 F SED (MSD) 41758 Lab Blank 41758 Spiked Matrix 41758 M SED (MS) 41758 M SED (MSD) 38714 Lab Blank 38714 Spiked Matrix 38714 530 SED (MS) 38714 41820 530 SED (MSD) Lab Blank 41820 Spiked Matrix 41756 Lab Blank AXYS ID WG40114101 WG40114102 WG40114103 WG40114104 WG41757101 WG41757102 WG41757103 WG41757104 WG41758101 WG41758102 WG41758103 WG41758104 WG38714101 WG38714102 WG38714103 WG38714104 WG41820101 WG41820102 WG41756- Sample (g) 5.00 UNITS ng/g % Recov 5.33 ng/g (dry wt) 5.25 ng/g (dry wt) 5.00 ng/g % Recov 5.18 ng/g 5.25 ng/g 5.00 ng/g % Recov 5.21 % Recov 5.15 % Recov 5.00 ng/g % Recov 5.36 % Recov 5.38 % Recov 0.500 ng/g % Recov 2.00 ng/g PFBA < 0.100 PFPeA < 0.100 90 87.5 42.3 46 41.5 47.6 < 0.100 < 0.100 125 113 55.5 58 57.9 55.9 < 0.100 < 0.100 130 117 123 127 129 131 < 0.100 < 0.100 87.3 84.7 94.2 94.6 95.3 102 < 1.00 < 1.00 106 107 < 0.500 < 0.500 PFHxA < 0.100 94.7 43.4 45.8 < 0.100 128 60.2 60 < 0.100 141 124 125 < 0.100 89 88.2 94.1 < 1.00 115 < 0.500 PFHpA < 0.100 87.5 45.6 46.4 < 0.100 109 60.8 56.8 < 0.100 114 129 131 < 0.100 94.2 97.8 106 < 1.00 107 < 0.500 PFOA < 0.100 93.8 41.9 46.2 < 0.100 117 58.3 57.5 < 0.100 131 122 120 < 0.100 83.8 98.8 101 < 1.00 105 < 0.500 PFNA < 0.100 92.7 40.3 44.3 < 0.100 119 55.5 56.4 < 0.100 139 123 129 < 0.100 70 89.7 93.9 < 1.00 105 < 0.500 PFDA < 0.100 104 44.2 46.1 < 0.100 127 57 57.9 < 0.100 155 126 121 < 0.100 106 94.3 93.3 < 1.00 120 < 0.500 PFUnA < 0.100 60.1 33.6 28.4 < 0.100 103 51.5 47 < 0.100 105 106 115 < 0.100 75.8 57.3 53.7 < 1.00 109 < 0.500 PFDoA < 0.100 90.8 38.2 40 < 0.100 128 52 50.6 < 0.100 130 113 108 < 0.100 94.7 84.8 85.9 < 1.00 120 < 0.500 PFBS < 0.200 94 86.2 88.2 < 0.200 121 112 112 < 0.200 129 116 105 < 0.200 90.6 96.8 87.8 < 2.00 118 < 1.00 PFHxS < 0.200 88.5 87 91.3 < 0.200 121 117 120 < 0.200 129 119 111 < 0.200 87.9 92.3 85.5 < 2.00 113 < 1.00 PFOS < 0.200 85.5 82.5 82 < 0.200 113 125 113 < 0.200 125 128 127 < 0.200 91.7 101 96.4 < 2.00 106 < 1.00 PFOSA < 0.100 64.1 43.1 37.1 < 0.100 103 60.6 55.2 < 0.100 105 132 131 < 0.100 64.5 62.2 57.2 < 1.40 104 < 0.600 Mois ture (%) 20.7 21.8 28.7 28.4 25 25.3 37 36.4 Perfluorochemicals in Mississippi River Pool 2 May 2013 64 Minnesota Pollution Control Agency WORK GROUP CLIENT ID 41756 Spiked Matrix 41756 E INV (MS) 41756 E INV (MSD) 38738 Lab Blank 38738 Spiked Matrix 38738 524 INV (MS) 38738 524 INV (MS) 38738 38738 40110 524 INV (MSD) 524 INV (MSD) Lab Blank 40110 Spiked Matrix 40110 40110 P2_525_INV (MS) P2_525_INV (MSD) AXYS ID 101 WG41756- 102 WG41756- 103 WG41756- 104 WG38738- 101 WG38738- 102 WG38738- 103 WG38738- 103 WG38738- 104 WG38738- 104 WG40110- 101 WG40110- 102 WG40110- 103 WG40110- 104 Sample (g) UNITS % Recov 1.34 % Recov 1.33 % Recov 2.00 ng/g % Recov 2.00 ng/g (wet wt) 2.00 % Recov 2.04 ng/g (wet wt) 2.04 % Recov 0.500 ng/g % Recov 0.370 0.390 ng/g (wet wt) ng/g (wet wt) PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA Mois ture (%) 106 93.7 105 96 109 106 125 98.2 113 112 116 108 104 113 466 116 228 106 109 130 435 115 50.6 108 94.5 116 113 489 111 228 108 106 124 429 115 50.6 121 95.8 118 < 0.250 < 0.250 < 0.250 < 0.250 < 0.250 < 0.250 < 0.250 < 0.250 < 0.250 < 0.500 < 0.500 < 0.500 < 0.350 94.7 99.8 108 103 105 94.6 123 110 107 107 105 105 97.9 10.7 8.07 11.3 12.1 11.2 9.75 11.9 12.9 11.1 21.7 21.3 D 20.1 9.54 107 80.7 113 121 112 97.5 119 129 111 108 107 101 95.4 10.2 8.21 10.5 11.5 11 9.89 11.4 12.7 10.9 19.4 20.8 D 22.8 9.84 104 83.8 107 117 112 101 116 129 111 99.1 106 116 100 < 1.00 < 1.00 < 1.00 < 1.00 < 1.00 < 1.00 < 1.00 < 1.00 < 1.00 < 2.00 < 5.00 < 2.00 < 1.40 101 79.5 103 97.7 109 105 104 104 100 112 106 95.1 102 53 D 94.8 D 48.1 D 54.2 55.7 53.6 54.3 75 54.7 D 50.1 105 111 49.7 53.3 D 98.0 D 52.5 D 57.6 53.3 57.9 56.8 88.4 53.7 D 50.8 116 114 49.5 Perfluorochemicals in Mississippi River Pool 2 May 2013 65 Minnesota Pollution Control Agency