Document rpJOak728dE7XRqkn7Lv9GG7q

CHRONIC TOXICITY OF EFFLUENT FROM VISTA CHEMICAL COMPANY, VCM PLANT . TO THE FATHEAD MINNOW (Pimephales promelas) IN A STATIC RENEWAL BIOASSAY EN89270 Performed for: Vista Chemical Company Attn: Paul Fetzer VCM Plant Road Westlake, LA 70669 on September 11-18, 1989 Performed by: ENSR Consulting and Engineering (formerly ERT) Environmental Laboratory 2400 West Loop South Suite 300 Houston, TX 77027 0O0O4I?9 EN5R Formerly ERT CHRONIC TOXICITY OF EFFLUENT E!NSR Consulting and Engineering 2400 W est Loop South Suit<* 300 Houston. T\ 77027 (713) Q60-8o57 FROM VISTA CHEMICAL COMPANY, VCM PLANT TO THE FATHEAD MINNOW (Pintephales promeUs) IN A STATIC RENEWAL BIOASSAY EN89270 Performed for: Vista Chemical Company Attn: Paul Fetzer VCM Plant Road Westlake, LA 70669 on September 11-18, 1989 Performed by: ENSR Consulting and Engineering (formerly ERT) Environmental Laboratory 2400 West Loop South Suite 300 Houston, TX 77027 REPORT FORMAT Version 3.2 July, 1989 VVV 000004130 1 1 TABLE OF CONTENTS SECTION 1 1. EXECUTIVE SUMMARY PAGE i 1 2. INTRODUCTION 3. FACILITIES AND STAFF 1 1 1 4. METHODS & MATERIALS 2 5. RESULTS 4 1 Table 1. Survivorship during the 7-day static renewal test. 5 1 Table 2. Summary of survival data of fathead minnow larvaeexposed for7 days to the effluent. 6 1 Figure 1. Flow chart for statistical analysis of chronic toxicity test data. From EPA (1985b;1988:30;1989:9). 7 1 6. DISCUSSION 8 1 7. LITERATURE CITED 9 8. QUALITY ASSURANCE/QUALITYCONTROL CHECKLIST AND CERTIFICATION 10 I 9. APPENDICES A. Sample Receiving Form I B. Chain-of-Custody C. Chemical Analysis of Tap Water, Deionized Water, and Saltwater Used in ENSR Environmental I Toxicology Lab - Houston D. Initial Characterization of Effluent and Receiving Water E. Water Quality Parameters Lab Notebook I I J J J I VVV 00000413! 1 1 1. EXECUTIVE SUMMARY 1 1.1 OBJECTIVE: The objective of this study was to determine the chronic toxicity to Pimephales promelas of an effluent submitted by Vista Chemical Company. 1 1.2 SAMPLE DATES: The composite samples were collected by Vista Chemical Company personnel and received at ENSR 1 Consulting and Engineering Environmental Toxicology Lab on 9/11, 9/13, 9/15/89. 1.3 TEST DATES: A 7-day static renewal test was conducted 1 September 11-18, 1989. 1.4 TEST PROTOCOL: The test was conducted according to methods 1 specified by the U. S. Environmental Protection Agency (1985b, 1989), Method 1000.0. 1.5 TEST CONDITIONS: The test was conducted at a temperature of 251*C 1 and a light intensity of 50 to 100 foot candles with a photoperiod of 16-hr light and 8-hr dark. 7 dilutions were tested: 26%, 52%, 59%, 67%, 76%, 86% and 100%. 76% is the critical concentration for pass/fail determination. 1.6 STANDARD TOXICANT: 24-Hour LC50 = 9.2 parts per thousand (ppt) Sodium Chloride (NaCl). I 95% Confidence Limits * 8-11 ppt. 1.7 DILUTION WATER: Receiving Water (Bayou Verdine) I 1.8 DATA ANALYSIS: This set of data could not be analyzed because of 92% control mortality. I 1.9 TEST RESULT$-LC50; An LC5q could not be calculated from this set of data I 1.10 TEST ACCEPTABILITY: Control 1 survival, 8%, does not meet minimum 80% survival. ] 1.11 CONCLUSIONS AND COMMENTS: Laboratory water control survival (85%) and growth (0.30 mg/fish) demonstrated that the fish used in this test were suitable for toxicity testing. J J VISTA CHEMICAL COMPANY EN89270 vvv 000004132 1 1 2. INTRODUCTION The objective of this study was to determine by a 7-day static renewal test 1 the chronic toxicity to fathead minnow (Pimephales promelas) larval survival and growth of an effluent. Samples were collected by the permittee and shipped to ENSR Consulting and Engineering Environmental 1 Toxicology Lab - Houston, Texas. The 7-day chronic toxicity test was performed September 11-18, 1989 in the Environmental Toxicology Laboratory at the ENSR Environmental Lab. This report follows the recommended outline 1 by EPA (1985b, 1989) for chronic toxicity reports. All data, laboratory notebooks, and associated documentation are archived at ENSR Environmental Toxicology Lab for future reference. 1 3. FACILITIES AND STAFF 1 All toxicity testing at the ENSR Environmental Lab was performed in the Environmental Toxicology Laboratory (approximately 3,000 sq. ft.). Test organisms were cultured or acclimated to test conditions in the Aquatic 1 Culture Facility (1,000 sq ft) separate from the Environmental Toxicology Laboratory. Both labs are located in the basement of the ENSR Consulting and Engineering building, constructed of 8" thick concrete walls and designed to minimize the effects of changing ambient climatic conditions. 1 All chemical tests were performed in the Chemistry Laboratory (500 sq ft) located on the fourth floor of the same building or at ENSR's Chemistry laboratory (2925 Richmond Avenue, Houston, TX 77098). All areas of the ENSR Environmental Toxicology Lab have separate, dedicated environmental control systems to avoid any possible air or water cross-contamination between facilities. A Sample Receiving Center (SRC) is located adjacent to the building's loading dock. It includes numerous laboratory refrigerators and freezers for sample storage at 41*C. All samples were assigned a unique, coded number upon receipt and the progress of the sample was tracked on a computer sample tracking system (MASS-11) designed for ENSR I Environmental Lab. The Environmental Laboratory is supplied with fresh water for toxicity testing from a 1,000 gallon freshwater system. Fresh water is aerated and I conditioned prior to use and is filtered through a series of physical (5 micron), chemical, and biological filters, then oxygenated. Water is chemically analyzed quarterly for various inorganic and organic parameters. I Two high capacity Millipore Deionized water systems provide high quality makeup water. A Millipore Q Distilled Water system provides distilled water of even higher quality water for phytoplankton cultures or chemical J analyses. All biological testing was performed in a Nor-Lake Scientific (2'x6.5') or two Bally self-contained Environmental Chambers (12'xl8' and 8'xl0') with dedicated microprocessor control systems capable of maintaining j j . VISTA CHEMICAL COMPANY -1- EN89270 vvv 000004133 1 1 temperatures within 1.0*C in a temperature range of 5-30*C. Temperature is displayed digitally and recorded on 7-day Partlow recording thermometers for documentation. A hi/low temperature alarm system is built-in. 1 Lighting is provided by cool-white vaporproof fluorescent lights at an intensity of 70-foot candles. Light and dark photoperiods are maintained by Dayton automatic timers. Air is supplied by an oil-less Rotron Air 1 Blower. An emergency backup power generator maintains the supply of air during power shutdowns. 1 Test species raised in the culture facility have been taxonomically verified and voucher specimens are maintained. Where possible, the original source of the test organisms was from EPA stocks. Genetically characterized strains are maintained for various species. All culture 1 stocks are tested monthly and quarterly with a standard reference toxicant for the development of a cumulative database through successive generations. Culturing of test organisms and the performance of all toxicity testing is carried out by a staff of degreed biologists. Biological and chemical 1 analyses are performed using appropriate EPA protocols. Quality control and quality assurance audits are performed by the QA/QC Officer of the Environmental Laboratory. 4. METHODS AND MATERIALS The type of plant, operating schedule, final products, schematic of wastetreatment, discharge information, flows, etc. have been previously submitted to the EPA by the permittee in EPA Form 2C. Composite samples of the effluent were collected by the permittee, immediately placed on ice in a cooler and transported to Houston (Appendix I A). Chain-of-custody procedures were observed and the completed documents are attached (Appendix B). Effluent samples were stored at 4C. I Dilution water was receiving water. Appendix D describes the chemical characteristics of the effluent and receiving water. Testing was carried out according to procedures specified in permittee's I permit and Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms (EPA, 1985b, 1989). I Test organisms were fathead minnow {Pimephales promelas) larvae less than 24-48 hrs old, as per the EPA freshwater chronic manual (1989). The larvae were exposed in a static renewal test for 7 days to 7 different effluent J concentrations 26%, 52%, 59%, 67%, 76%, 86%, 100% and 2 controls. Control 1 (dilution water) was receiving water (Bayou Verdine) and Control 2 was moderately hard lab water. J J . VISTA CHEMICAL COMPANY -2- EN89270 GOOo 0*134 1 1 Renewal solutions (effluent concentrations) were prepared the day that each composite sample arrived at ENSR Environmental Toxicology Lab. A sufficient quantity of each test solution was prepared for three days of testing 1 (i.e., three consecutive renewal periods). The reservoirs of test solution were stored at 4'C. 1 All glassware was cleaned according to procedures specified in Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms (EPA, 1985b, 1989) and described in detail 1 in "ENSR Consulting and Engineering, ENSR Environmental Laboratory Standard Operating Procedures Manual. (September 1988; revised May, 1989). The test chambers were Nitex cylinders placed inside 1-liter beakers. The 1 Nitex cylinders were constructed by attaching a 7-cm high collar of 363micrometer mesh Nitex screen to a 15x60 mm glass Petri dish with silicone sealant. Larvae were placed into the cylinders in random order until 10 1 larvae were in each cylinder. The test was initiated by randomly placing a Nitex cylinder containing 10 larvae into the test chamber. A total of 40 larvae was tested for each 1 concentration (4 replicates per concentration). The light quality and intensity were at ambient laboratory levels of 50 to 1 100 foot candles, with a photoperiod of 16-hour light and 8-hour dark (16L:8D). The water temperature in each test chamber was maintained at 251*C. The larvae in each test chamber were fed newly hatched (<24-hr I old) brine shrimp nauplii three times daily: at the beginning, midway and at the end of each day. The nauplii were soaked in fatty acid, vitamin and trace element solution to enhance their nutritional value. The nauplii were rinsed with moderately hard lab water just prior to feeding. I The number of live larvae in each test chamber were recorded daily and the dead larvae discarded. Just prior to the daily renewal of the test I solutions, uneaten and dead brine shrimp and other debris were carefully removed from the bottom of each Nitex cylinder with a new polyethylene dropper. I Test solutions were renewed by gently lifting the Nitex cylinders out of the test chambers and placing them into new test chambers (1-liter beakers) I containing new test solutions (250 mL). During the transfer, the larvae remained in the solution filled petri dishes at the bottom of the Nitex cylinders. I This procedure for the daily renewal of the test solutions was observed to be far less stressful to the larvae than the recommended siphon technique (Method 1000.0; EPA, 1985b) which often leads to the inadvertent removal of J larvae (i.e. caught up in the siphon). The test solution volume was also reduced from the recommended 500 mL (EPA 1000.0) to 250 mL per replicate to reduce the volume of sample required for J the test. This alternative is consistent with the testing protocol used by EPA Region VI Toxicity Testing Laboratory, Houston, Texas and the second edition of the EPA manual (1989). J J VISTA CHEMICAL COMPANY -3- EN89270 WV 000004135 1 1 Dissolved oxygen was measured at the beginning and end of each 24-hr exposure period at all test concentrations and in the control. 1 Temperature, pH, and conductivity were measured at the beginning of each 24-hr exposure period at all test concentrations and in the controls. This information is contained in Appendix D. 1 The test was terminated after seven days of exposure. At termination, the live larvae in each test chamber were counted and preserved as a group in 4% formalin or 70% ethanol. For the dry weight analysis, the preserved 1 larvae from each test chamber were rinsed in deionized water, transferred to tared weighing boats and dried at 100*C for a minimum of 2-hrs. Immediately upon removal from the drying oven, the weighing boats were 1 placed in a dessicator. Weights were measured to the nearest 0.10 mg. All data were analyzed according to the statistical flow chart outlined in the second edition of the EPA Freshwater Chronic Methods Manual (1989)* 1 Figure 1 is a generic flow chart of the statistical approach used in this report. Statistical analyses are included in Appendix E. 1 Survival and growth data from the fathead minnow larval survival and growth test are analyzed using hypothesis testing techniques. TOXSTAT, TOXDAT and a proprietary computer program are used for all calculations. Survival data are arc-sine transformed and both data sets are tested for normality 1 by Shapiro-Wilk's test. Normal data are then tested for homogeneity of variances by Bartlett's test. Data passing both tests are analyzed by Dunnett's test (Dunnett, 1955). Dunnett's test is a two stage test and consists of an analysis-of-variance (ANOVA) to determine if treatment means are different from each other. If treatment means are significantly different, then the second test, a post-hoc multiple-range comparison is made between the treatment means and the control mean. If treatment means I are equal, the post-hoc comparison is not performed (Snedecor and Cochran, 1980). I Data that do not meet the normality assumptions are analyzed by a nonparametric test, Steel's Many-One Rank test. If an unequal number of replicates exist, Wilcoxon Rank Sum test with Bonferroni's Adjustment is I used alternatively. A modified version of the TOXDAT program is used to calculate an LC50 if mortality occurred in the higher test concentrations. The LC50 is calculated by three different statistical models (binomial, moving-average, and probit) and the model fitting the data best is used. LC50's are corrected for control mortality by a maximum likelihood estimation method. I The LC50 is reported as an unadjusted value. J 5. RESULTS - Effluent The results derived from the 7-day static renewal test with the fathead minnow {Pimephales promeTas) larvae and effluent are shown in Table 1. J Table 2 lists the percent survival in replicate chambers and the mean percent survival at 24-hr (Day 1), 48-hr (Day 2) and 7-days. The test J results were not acceptable with survival in the controls less than 80% (EPA, 1985b, 1989). VISTA CHEMICAL COMPANY -4- EN89270 VVy 000004i36 Table 1. Survivorship during the 7-day static renewal test Test Day Proportion Cone.[%) 0 1 2 3 4 5 6 7 Surviving 0 % A 10 10 10 9 8 5 2 1 0.10 CONTROL 1 B 10 10 10 10 10 7 3 1 0.10 C 10 10 10 10 9 4 1 1 0.10 D 10 10 10 10 9 5 2 0 0.00 26 % A 10 10 6 6 6 1 0 0 0.00 B 10 10 10 9 7 2 1 0 0.00 C 10 10 10 9 9 4 1 0 0.00 D 10 10 10 10 9 4 2 0 0.00 52 % A 10 10 8 2 2 0 0 0 0.00 B 10 10 7 2 1 0 0 0 0.00 C 10 10 8 7 6 1 1 0 0.00 D 10 10 10 7 7 1 1 0 0.00 59 % A 10 8 8 0 0 0 0 0 0.00 B 10 10 7 1 1 0 0 0 0.00 C 10 10 8 2 2 1 1 0 0.00 D 10 10 7 1 1 0 0 0 0.00 67 % A 10 10 9 2 2 2 0 0 0.00 B 10 7 6 1 1 0 0 0 0.00 C 10 8 5 3 2 0 0 0 0.00 D 10 9 7 2 1 1 0 0 0.00 76 % A 10 9 5 0 0 0 0 0 0.00 B 10 9 7 0 0 0 0 0 0.00 C 10 9 6 0 0 0 0 0 0.00 D 10 7 5 2 0 0 0 0 0.00 86 % A 10 8 2 0 0 0 0 0 0.00 B 10 7 2 0 0 0 0 0 0.00 C 10 8 2 0 0 0 0 0 0.00 D 10 7 2 0 0 0 0 0 0.00 100 % A 10 7 1 0 0 0 0 0 0.00 B 10 5 4 0 0 0 0 0 0.00 C 10 6 0 0 0 0 0 0 0.00 D 10 6 1 0 0 0 0 0 0.00 CONTROL 2 A 10 10 10 10 10 9 9 9 0.90 B 10 10 10 10 10 10 10 10 1.00 C 10 10 10 9 6 6 6 6 0.60 D 10 10 10 10 10 10 9 9 0.90 VISTA CHEMICAL COMPANY -5- EN89270 VVV 00000*137 i FATHEAD MINNOW Table 2. Summary of survival data of fathead minnow larvae exposed for l 7 days to the effluent. Cone.(%) Replicate ABC Time D 24 H 48 H 7 D CV* 0% 0.10 0.10 0.10 0.00 1.00 1.00 0.08 58 i 26 % 0.00 0.00 0.00 0.00 1.00 0.90 0.00 52 % 0.00 0.00 0.00 0.00 1.00 0.83 0.00 59 % 0.00 0.00 0.00 0.00 0.95 0.75 0.00 i 67 % 76 % 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.85 0.68 0.00 0.85 0.58 0.00 86 % 0.00 0.00 0.00 0.00 0.75 0.20 0.00 100 % 0.00 0.00 0.00 0.00 0.60 0.15 0.00 CONTROL 2 0.90 1.00 0.60 0.90 1.00 1.00 0.85 18 i * CV = StDev*100/Mean i The survival data were analyzed with the EPA TOXDAT Program. TOXDAT could not calculate an LC50 due to 92% control mortality. Data sets were not analyzed because of dilution water (receiving water) toxicity. I J i VISTA CHEMICAL COMPANY -6- EN89270 WV 000004133 1 1 1 1 1 1 1 1 I I I I J J FIGURE 1 FLOW CHART FOR STATISTICAL ANALYSIS OF CHRONIC TOXICITY TEST DATA MODIFIED FROM EPA (1988. 1989) VVV 000004139 6. DISCUSSION The response of a subsample of the fathead minnow larvae population used in this toxicity test to a reference (standard) toxicant (NaCl), as well as survivorship in the laboratory water control showed that the test organisms were in suitable condition for use in toxicity testing. ENSR Environmental Toxicology Lab routinely runs bioassays with a reference toxicant to determine the sensitivity of test organisms. When compared with historical standard toxicant results, these data indicate the similarity in sensitivities of test organism populations at different times and thus the extent to which tests performed with the same species at different times may be considered comparable. All requirements of the prescribed protocol (EPA, 1985a) were adhered to and conditions were maintained within the required limits throughout the test. VISTA CHEMICAL COMPANY -8- EN89270 VVV 000004140 1 1 7. LITERATURE CITED Dunnett, C.W. 1955. Multiple comparison procedure for comparing several 1 treatments with a control. J. Amer. Stat. Assoc. 50:1096-1112. EPA, 1985a. Methods for measuring the acute toxicity of effluents to 1 freshwater and marine organisms. EPA/600/4-85/013. Environmental Monitoring and Support Lab. Cincinnati, Ohio. Third Edition. 216 pp. 1 EPA, 1985b. Short-term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms. EPA/600/485/014. Environmental Monitoring and Support Lab. Cincinnati, Ohio. 162 pp. 1 EPA, 1988. Short-term methods for estimating the chronic toxicity of effluents and receiving waters to marine and estuarine organisms. Environmental Monitoring and Support Lab. Cincinnati, OH. EPA 600/4-88028. 417 pp. 1 EPA, 1989. Short-term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms. Environmental Monitoring and Support Laboratory. Cincinnati, OH. EPA 600/4-89-001. February, 1989. 250 pp. Snedecor, G.W., and W.G. Cochran. 1980. Statistical Methods. Seventh Edition. Iowa State University Press. Ames. 593 pp. I i i j 1 i j , VISTA CHEMICAL COMPANY -9- FN89270 Vvv 00004l4i 8. QUALITY ASSURANCE/QUALITY CONTROL CHECKLIST AND CERTIFICATION ENSR CONSULTING AND ENGINEERING ENVIRONMENTAL LABORATORY - HOUSTON QUALITY ASSURANCE / QUALITY CONTROL PROCEDURES CHECKLIST FATHEAD MINNOW LARVAL SURVIVAL AND GROWTH TEST METHOD 1000 (EPA-600/4-85/014, FEB 1989) (Section numbers in parentheses refer to method section(s) where requirement is discussed.) A. Sample Characteristics Yes No 1. Sample quality maintained according to protocol requirements (8.0)? 2. Sample tested within time period required by protocol (8.3.1)? 3. Salinity measured to determine if freshwater (5 ppt) test required? B. Testing Conditions 1. Standard toxicant LC50 is reasonable? 2. <24-48 hr old larvae acclimated (if necessary) 3. Test temperature maintained at 251*C? 4. Dissolved oxygen concentration >40% saturation? 5. Aeration required? 6. Protocol followed without deviation? C. Test Acceptability Criteria 1. Laboratory water controls a. Average survival >80%? b. Average dry weight of preserved larvae >0.25 mg/fish? 2. Receiving water controls a. Average survival >80%? b. Average dry weight of preserved larvae >0.25 mg/fish? 3. Coefficient of variation between replicates <40% for survival? 4. Coefficient of variation between replicates <40% for growth? D. Report 1. Report pages are numbered in sequence? 2. Each page contains the sample number? 3. Page numbers listed in the table of contents correspond to pages in the body of the report? VISTA CHEMICAL COMPANY -10- EN89270 000004142 1 1 4. Headings listed in the Table of Contents correspond to headings in the body of the report? 1 5. Titles of tables listed in the Table of Contents correspond to tables in the body of the report? 1 6. Titles of figures listed in the Table of Contents correspond to figures in the body of the report? 1 7. Values appearing more than once in the text agree? 8. Tables and figures appear in the order in which they are referenced in the report? 1 9. Horizontal tables and figures are bound above the top edge? 10. Sample conditions upon receipt adequately 1 reported? 11. Explanations are provided for all footnoted items? 1 12. Units are provided for all columns and rows of data? 13. Appendices attached to report? A. Sample Receiving Forms I B. Chain-of-Custody forms C. Chemical analysis of tap water, deionized water and saltwater used in ENSR ! Environmental Laboratory - Houston D. Initial characterization of effluent and receiving water E. Water Quality Parameters Lab Notebook I F. Statistical Analyses of Data I E. COMMENTS I I I I J J P VISTA CHEMICAL COMPANY j -11- EN89270 VV* 000004143 CERTIFICATION Data presented in this report were derived by methods and materials identified in the section of the report entitled "Methods and Materials," in accordance with the protocol specified by the U.S. Environmental Protection Agency (1985b, 1989). All laboratory practices and procedures were in accordance with ENSR Environmental Toxicology Lab Standard Operating Procedure and the ENSR Environmental Toxicology Lab Standard Operating Procedures Manual, (September 1988, May 1989). A project notebook was maintained during the testing program, and all data transcribed from the notebook to the report were checked for accuracy by two people. Hugh Johnson, Se6fcTion Manager Freshwater Biomcmitoring Section ENSR Environmental Toxicology Lab QA/QC Officer, ENSR Environmental Toxicology Lab HJ/mr 23-OCT-89 VISTA CHEMICAL COMPANY -12- EN89270 VVV 000004144 1 ] 1 1 1 1 1 1 APPENDIX A | Sample Receiving Form i i i i j j i I 1 MV OOOQOA145 1 jjECT #: rnff : SAMPLE C1IECK-III FORM ~00X'<r*,9 U/77H- (eff\ * ' : - 1 ---- UJMITY : -j--------------- J ,1j_ : I 1 IMP `C : : ik fa JhLucTIVITY : -ACK SPOTS t -ipR H. tn 7.3 G.o TOOO IS H"yes B/yes H^YES Iff YES 1 ['] YES U NO [] NO U NO [] NO H'NO- juL odor # \HPLE )i ("A I HER eoly [] GLASS ( .[] 1 gallon Letlca pai 1 [] 500 ml [] 1000 lnl VES y-Ho [tf'DlHER 5 4AL-. CUOE [] OTHER i. jl KIT: [] yes [ifno yot, describe: M'lgloo Cooler __________ M 1|0 ICE: [J frozen coo 1 [] no Igloo Ice v. [1 styrofoam cooler H [] warm 1-illT % (vo,/vol); ! % water f] yes r pr : [J yes ['T'no % on [<Kno % sol Ids Sicmed WV 00000A146 D.-.FP SAMPLE CHECK-IU FORM "h/JECT X&fS-OOl.-n'S? EV ! V7V>o.on. T : i/n'/fi. SALINITY : j )0 : 11 ill : LHP `C : *" * TlA 73 1.0 V EkYES [(LYES tmUUCTIVITY : 3750 , KMES LACK SPOTS : oJl uUOR : / / n yes U YES [] NO U NO [] NO [] NO [ijdlO [4^H0 AriPLE jjrAINER [] POLY [] GLASS [] 1 gallon Letica pail [] 500 ml [] 1000 nil [fTOlliEH 5-4/m, daQE[] OTHER J jl KIT: [] yes pflio jiot, describe: [tj^Igloo Cooler [] styrofoam cooler [] LOO ICE: -* [J frozen [l^coo! [] no Igloo Ice [] warm lJUT % (vol/vol J: [] yes % water [J yes % oil [ij^no % sol ids S icmed 9/u/irf VVV 000004147 111 > n 1 . SAMPLE CHECK-IH FORM I V*" '' ' -, ' ~j .>1;-C6U15 -00^ -03^- ____________________ \) Vo v t \ - v 10 i ^%c\210 -O 1 _ *1 i 1. i * i , \ * C4 c,mo-vM';. mE crli3M " ;AL1HITY : "I )0 t 1] 'll 3 P *C : J UUCTIV.ITY : L/ CK SP0T5 : . 5 s.l ' 1.2. ' 1 WO yj bi VES ' f/ VES [J YES [Ji YES 1 [] YES U NO t] NO U NO [] NO ti NO 01IL UUOIl [] yes [{ NO At- PLE ci TAINER U poly [] GLASS ;.u 1 gallon Letica pail [] 500 ml [] 1000 ml t] OTHER j TMi R KIT: ^-H'yes [J no F|not, describe: \J\ Igloo Cooler -j v. N styrofoam cooler n 1 JO ICE: [] frozen \/\ cool [J warm [] no Igloo ice :nmr % */vol J: L_____ _ % water [] yes 1 tJ yes [J] no % oil ll no % sol ids J X /?V / // n 1__ t 000004143 SAMPLE CHECK--III FORM jjlyfo 1 ................................. / -> \TE ' ` * -l-- U.INITY s -i-- T 3-c , jUCTIYITY s AJK SPOTS : Ol .JOR N^LE tJ'UHER : U POLY [] GLASS %mp.p 1A. (? i iz^OO "[J 1 gallon Letlca pall [] 500 nil [] 1000 ml [^ES H^ES HOfES U YES YES [J--TTlER )2L ~ [] OTHER S I KIT: yes [] no t] NO U NO [] NO NO (pi) G&/ 1 1 1 1 1 1 1 1 APPENDIX B i Chain-of-Custody i i i j j j i VVV 000004150  Lake Charles Chemical Plant Chaln-ol-Custody Record PROJECT NAME/NUMBER SAMPLE TEAM MEMBERS 4/____-S/C r*y*~ viatd iwiidm^di uuiu^auy Lake ^rles VCM Plant --Dl _J ____ I Lake Charles LAB Plant R/A Control No. \ \ C/C Control N .01441 LAB DESTINATION CARRIER/WAYBILL NO. Sample Number 1 Sample Location and Description OOf Ouf^/f Z 0O\ Gui-fn f I GO} >ai~Fa.ll OO/ 0,1"^raJ/ r*> 0 / cnGrFq cL. Date and Time Collected Sample Type q-io-M-ouiD r 7'(('Vt/es>a * Container Type Condition on Receipt (Name and Date) Disposal Destination Special Instructions:_____ Possible Sample Hazards: 00000415 l LiQBltsrM U"3T|': Q"'wffw ChiiewwChernwHW'lant --p"Lake->"fuiies Vwrrr-tanl l_T!akB OlwnJs LABTfftllt Chsin-of-Custody Record r^fBErTTo XtCiij PROJECT NAME/ SAMPLE TEAM MEMBERS --Q& / Sample Number 9- /6-Y9 -- Sample Location and Description Oa/ "(%&*** Date and Time Collected efati - 3i<o- rv LAB DESTINATION CARRIER/WAYBILL NO. Sample Type Cr Container Type AL&Z R/A Control No. C/C Control N _ 01444' Condition on Receipt (Name and Date) Disposal Destination (UrtnOdS-t/c jnxlc- Fv^ & J>e e CAl Mj o/<t 9/ Special Instructions: Possible Sample Hazards: SIGNATURES: (Name, Company, Date and Time) 3. Relinquished By;. Received By:____ 4. Relinquished By; V8-65-X3 Received By:____ Whit - To Accompany Samples Canary - Laboratory Copy The white copy Is to be returned to the Environmental Coordinator at the plant of origination after the analysis is completed. Pink Sample Team Copy ? S T * 0 0 0 0 0 AAA  Lake Charles Chemical Plant Lake tries VCM Plant Lake Charles LAB Plant v IV* Chain-of-Custody Record PROJECT NAME/NUMBER SAMPLE TEAM MEMBERS Sample Number Sample Location and Description \liirjt nS>yri)JL * (T / vi Kt\a t-L Date and Time Collected J-tWi <jr3 LAB DESTINATION CARRIER/WAYBILL NO. Sample Type Container Type 9UU. R/A Control No. C/C Control N .01445' Condition on Receipt (Name and Date) Disposal Destination Special Instructions:_____ Possible Sample Hazards: SIGNATURES: (Namlee, CQopmmppaannyy,.Dpaate and Time) 1. Relinquished By: 9-rZ't? 3. Relinquished By: Received ByiS\ ^ i^\ I u (, 2. Relinquished Received By: vj V0-S5-X3 . f-n->9 o frp Received By:___ <T-H~dF yP.VJ ^ 4. Relinquished By: IQ-'i Received By: White - To Accompany Sample* Canary Laboratory Copy The while copy Is to be returned to the Environmental Coordinator at the plant ol origination attar the analysis Is completed. Pink - Sample Team Copy w v 000004153  Lake Charles Chemical Plant Chofniuji Com^unj Lake . ..rles VCM Plant Chaln-of-Custody Record PROJECT NAME/NUMBER SAMPLE TEAM MEMBERS S.Cqa{aP* '2^zr LAB DESTINATION CARRIER/WAYBILL NO. Sample Number / ZL 3 v: r! Sample Location and Description oo/ OtotJL+JLQ. xO/ 0 o / qja'Msa 9f Date and Time Collected Sample Type f-/.J-g*7 0/5-0 t ( / ' -------- -- Container Type Condition on Receipt (Name and Date) Disposal Destination Special Instructions:_____ Possible Sample Hazards: SIGNATURES: (Name, p9mtjany, Date and Time) ft Relinquished By^l V A Received By l^bd cj-rL#!\ /So* 7-ja.-?? 3. Relinquished By: Received o/SQ 7-/3-&? tf/so 2. Relinquished By Received By: C^y LllftLAzlftzll'l 4. Relinquished By:. 'bPfffUa 7ms f-/2-p By: 'fdJ^/hbj? Received n'JZc - ft White - To Accompany Samples The white copy Is to be returned to the Environmental Coordinator at the plant of oriqination after the analysis Is completed, CpjannkaryS-amLapbleorTaetaomry CCooppyy WV 0 0 0 0 0 4 1 5 4  Lake Charles Chemical Plant \ Ch> I Cor_ l Laki irles VCM Plant I pjjy - I 'S Lake Charles LAB Plant Chaln-of-Custody Record PROJECT NAME/NUMBER jhaArftCtlj si</y SAMPLE TEAM MEMBERS Sample Number Sample Location and Description -rx -r/ /h o<js Date and Time Collected eivr * 4 0 9m LAB DESTINATION __________ CARRIER/WAYBILL NO___________________ R/A Control No. C/C Control N2 _01450 Sample Type Container Type Condition on Receipt (Name and Date) Disposal Destination fJ Special Instructions:_____ Possible Sample Hazards: oooqo C 4^ >'1r*\ SIGNATURES: (Name, Company, Date and Time) 1. Relinquished By: Z7 Us f# 9-q df&*> 3. Relinquished By: Received B1 ^ - 0vX~ y--___ $ Received By:___ 2. Relinquished B Received By: f // :3fA-K 4. Relinquished By: ?fn(sn7 //J-sr Received By:___ V8-65X3 White To Accompany Samples Canary - Laboratory Copy The white copy is to be returned to the Environmental Coordinator at the plant of origination after the analysis Is completed. Pink - Sample Team Copv  Lake Charles Chemical Plant Chain-of-Custody Record PROJECT NAME/NUMBER . 3ratbVt-i SAMPLE TEAM MEMBERS Cher Lake Oomf es VCM Plant LAB DESTINATION Lake Charles LAB Plant \ R/A Control No. \ C/C Control N .01451 &K?5(L CARRIER/WAYBILL NO.. Sample Number / Sample Location and Description Date and Time Collected Sample Type Container Type Condition on Receipt (Name and Date) Disposal Destination 3 ***?/?&/ 7 QST^OOODO AAA Special Instructions:_____ Possible Sample Hazards: SIGNATURES: (Name. Company, Date and Time) 1. Relinquished By: Received By 2. Relinquished By: /IMe .f-Ztf-fr__Lls. <?~IV'-X9 IS 3d 3. Relinquished Received By: 4. Relinquished Bfc. y-/r-gy o/DO f-n -- o l^> on:<ro Received By:___ Received Bv: 9-/S-& /iy.&3 V8-65-X3 Whin * To Accompany Samples Canary Laboratory Copy The white copy Is to be returned to the Environmental Coordinator at the plant of origination after the analysis Is completed. p|nk Sample Team Copy  Lake Charles Chemical Plant . Che Lake Corr fles VCM Plant Lake Charles LAB Plant Chaln-of-Custody Record PROJECT NAME/NUMBER 8/p (hillllLj________________ SAMPLETEAM MEMBERS LAB DESTINATION CARRIER/WAYBILL NO_____________ Sample Number yt-Ml Sample Location and Description CO I Date and Time Collected o"? T-tY-try ts CrXu^ 9-' S'irt Sample Type Ccrr~T* Container Type Condition on Receipt (Name and Date) Disposal Destination sJbuJY'*' fr ^ c/c 44, o r<fs / v i / Special Instructions: Possible Sample Hazards: _____________________________________________________________ SIGNATURES: (Name, Obmpany, Date and Time) 1. Relinquished By: ____ fate <>%Q Received hed 2. Relinquished Received V8-C5-X3 J)a^------^O^Ly tjj f ^ 9/^firf (nr Received By: White To Accompany Samples Canary - Laboratory Copy The white copy is to be returned to the Environmental Coordinator at the plant of origination after the analysis Is completed. Pink - Sample Team Copy t,tVOOOOO aaA APPENDIX C Chemical Analysis of Tap Water, Deionized Water, and Saltwater Used in ENSR Environmental Toxicology Lab - Houston 1 Chemical analysis of tap water, deionized water, and saltwater used In ENSR Environmental Laboratory. Specific analytical references, detection limits 1 and QA/QC results are available upon request. N.D. = Not Detected and N.M. Not Measured. 1 PARAMETER 1 Inoraanic Parameters Units Tap Water Deionized Saltwater Water (25 o/oo) Alkalinity 1 Hardness TDS TSS 1 Ammonia ICP Metals/Elements mg/L mg/L mg/L mg/L mg/L 210 100 280 N.D. 1.1 4 N.D. N.D. N.D. N.M. 62 4000 20,000 4 N.M. 1 A1uminum Cadmium Hexavalent Chromium 1 Copper Iron Lead I Manganese Mercury Selenium Zinc M9/L M9/L mg/L mq/l M9/L M9/L mg/L mq/l Mg/L m/l N.D. N.D. N.D. .04 0.4 N.D. 4.4 N.D. N.D. N.D. N.M. N.M. N.M. N.D. 0.08 N.M. N.M. N.M. N.M. 0.3 N.D. N.D. N.D. .03 0.06 53.4 N.D. N.D. N.D. N.D. ORGANOCHLORINE. Pesticides I ALPHA-BHC BETA-BHC DELTA-BHC i GAMMA-BHC (LINDANE) HEPTACHLOR ALDRIN I HEPTACHLOR EPOXIDE ENDOSULFAN I DIELDRIN I 4,4'-DDE ENDRIN ENDOSULFAN II 4,4.'-DDD I ENDRIN ALDEHYDE ENDOSULFAN SULFATE 4,4'-DDT ENDRIN KETONE j METHOXYCHLOR TOXAPHENE J AROCLOR-1016 AR0CL0R-IZZ1 AR0CL0R-1Z32 AROCLOR-1242 J AROCLOR-1248 AROCLOR-1254 AR0CL0R-1260 M9/L M9/L Mg/L Mg/L M9/L Mg/L Mg/L Mg/L Mg/L Mg/L Mg/L Mg/L Mg/L Mg/L Mg/L M9/L M9/L M9/L M9/L Mg/L Mg/L Mg/L Mg/L M9/L Mg/L /*9/L N.D N.D N.D. N.D. N.D. N.D N.D N.D N.D. N.D. N.D. N.D N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. VVV 000004159 PARAMFTER Units Tap Water Deionized Water Oraanic Parameters. Volatiles Chloromethane Bromomethane Vinyl Chloride Chloroethane * Methylene Chloride 1,1-Dichloroethane 1,1-Dichioroethane 1,2-Dichloroethene (total) Chloroform 1,2-Dichioroethane 1,1,1-Trichloroethane Carbon Tetrachloride Bromodichioromethane 1,2-Dichioropropane Cis-1,3-Dichioropropene Trichloroethene Dibromochloromethane 1,1,2-Trichioroethane Benzene Trans-1,3,-Diehloropropene 2-Chioroethylvinyl ether Bromoform Tetrachloroethene 1,1,2,2-Tetrachloroethane Tolvene Chlorobenzene Ethylbenzene Trichlorofluoromethane ml l 49/L m/\4g/L 49/l 49/l 49/l 4g/L 49/l 49/l 49/l 4g/L 4g/L m/l 49/l 49/L M9/L 49/L 4g/L Ag/L 49/L 49/L Mg/L 49/L 49/ L 49/1 A9/L A9/L N.D. N.D. N.D. * N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.O. N.D. N.D. N.D. N.D. N.O. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. Compound is present, but below the detection limit (10 49/L). Oraanic Parameters: Pthalate Fsters Dimethyl Phthalate Diethlyl Phthalate DI-N-Butyl Phthalate Butyl Benzyl Phthalate Bis(2-Ethyl hexyl)Phthal ate DI-N-Octyl Phthalate 49/L 49/L 49/149/L 49/L 0g/L N.M. N.M. N.M. N.M. N.M. N.M. Organic Parameters. Semivolatiles (Method 625) N.M. N.M. N.M. N.M. N.M. N.M. Benzo(k)fluoranthene bis(2-Chloroethoxy)methane bis(2-Chloroethyl)ether bis(2-Chloroisopropyl)ether bis(2-Ethyl hexylJphthalate 4-Bromophenyl phenyl ether Butyl benzyl phthalate 2-Chloronaphthalene 4-Chlorophenyl phenyl ether Chrysene 49/L 49/L 49/ L 49/L 49/ L 49/L 49/L 49/ L 49/ L 49/ L N.D. N.D. N.D. N.D. N.D. N.D. N.D, N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. Saltwater (25 a/ool N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.D N.D N.D N.D N.D N.D N.D N.D N.D N.D N.D N.D N.D N.D N.D N.D vvv 000004i60 1 1 PARAMETER Units Tap Water Deionized Saltwater Water (25 o/ool 1 Di benzo(a,h)anthracene 1,2-Diehlorobenzene m/l A9/L N.D. N.D. N.D. N.D. N.D. N.D. 1,3-Dichlorobenzene /ig/L N.D. N.D. N.D. 1 1,4-Dichlorobenzene 3,3'-Diehlorobenzidine A9/L m/l N.D. N.D. N.D. N.D. N.D. N.D. Diethyl phthalate /jq/l N.D. N.D. N.D. 1 Dimethyl phthalate Di-n-butyl phthalate 2,4-Dinitrotoluene /ig/L M9/L Mg/L N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 2,6-Dinitrotoluene 1 Di-n-octyl phthalate 1,2-Diphenyl hydrazine M9/L M9/L M9/L N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. Fluoranthene M9/L N.D. N.D. N.D. 1 Fluorene Hexachlorobenzene M9/L #*g/L N.D. N.D. N.D. N.D. N.D. N.D. Hexachlorobutadiene m/\- N.D. N.D. N.D. 1 Hexachlorocyclopentadiene Hexachloroethane Indeno(l,2,3-c,d)pyrene M9/L M9/L M9/L N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. Isophorone /ig/L N.D. N.D. N.D. Naphthalene M9/L N.D. N.D. N.D. Nitrobenzene A9/L N.D. N.D. N.D. N-Nitroso-dipropylamine A9/L N.D. N.D. N.D. N-Nitrosodiphenyl amine aq/l N.D. N.D. N.D. Phenanthrene Pyrene A9/L /ig/L N.D. N.D. N.D. N.D. N.D. N.D. 1,2,4-Trichiorobenzene M9/L N.D. N.D. N.D. 2-Chlorophenol A9/L N.D. N.D. N.D. 2,4-Dichlorophenol Atg/L N.D. N.D. N.D. 2,4-Dimethyl phenol W/L N.D. N.D. N.D. I 4,6-Dinitro-2-methyl phenol 2,4-Dinitrophenol M9/L /*g/L N.D. N.D. N.D. N.D. N.D. N.D. 2-Nitrophenol m/l N.D. N.D. N.D. I 4-Nitrophenol 4-Chloro-3-methylphenol /ig/L a*9/l N.D. N.D. N.D. N.D. N.D. N.D. Pentachlorophenol Atg/L N.D. N.D. N.D. Phenol I 2,4,6-Trichlorophenol Aig/t Aig/L N.D. N.D. N.D. N.D. N.D. N.D. 1 J ] \ Vvv 0000*IZ 1 1 1 1 1 1 1 1 APPENDIX D I Initial Characterization of Effluent and Receiving Water l I l VVV 000004162 1 1 Initial characterization of effluent and receiving water upon receipt and prior to mixing test concentrations. Sample Alkalinity Hardness Conductivity Temp. pH D.O. nh3 (Date Rec'd) (mg/L as CaC03) (pmhos/cm) (*C) (units) (mg/L) (mg/L) Effluent! EN89270.01 (9/11/89) 175 EN89270.03 (9/13/89) 164 EN89270.04 (9/15/89) 171 Receivina Water? EN89270.02 (9/11/89) 155 2674 2546 2525 1616 5000 6900 5500 3250 6.0 7.0 13.6 7.3 7.2 7.4 8.0 0.05 8.1 0.26 8.6 <0.01 7.0 7.3 4.9 0.05 ^Effluent is Vista Chemical Company. VCM Plant Outfall No. ^Dilution Water is Receiving Water. ^Receiving water is Bayou Verdine.. J i *vv 000004163 1 ] 1 1 1 1 1 1 APPENDIX E I Water Quality Parameters Lab Notebook I I I I I I ] j I VVV 000004164 1 1 ENSR 1 ENVIRONMENTAL LABORATORY - HOUSTON 1 Chronic Toxicity of Effluent to the Fathead Minnow (Pimephales promelas) under Static Renewal Test conditions. 1 EPA Method No: 1000.00 ENSR EL/Houston SOP No. 2; Revision 00, 9/88 1 ENSR EL/Houston Project No. fiZlS-oox-tiU_______ 1 ENSR EL/Houston Sample ID No.'s 1 Effluent Receiving &W`?X7Q.a3 Waters &AJ'K92-yo tox Date of Test: Time of Test: /73Q i Results Checked By: . f/s. I i ENSR EL/H Form No.: 001 Revision: 01 _s Revision Date: 3/89 WV 000004165 Page: _____of ENSR El/H Form No.: 002 Revision: 01 Revision Date: 3/80 SAMPLE LOG CLIENT VlTrfh Me/mcAL' Sample ID (Effluent) Sample ID (Dilution) Date Rec'd: Date(s) Used: Sampling Period #1 VmX70M__ EM?9X70,t>X V// 7/n `/l/xf/n PROJECT NO. 1&-c>ox-ox9 Sampling Period #2 wrist0 .0 3 Sampling Period #3 BtJV/lsraiH- nx E/jm-yoox i/n ?//Y 7/is' It* 1/n, 'ft ' Source of Dilution Water: Recb*/coajt&l ^/T Notes: c^/uiPal fy\h/LU> ------------------ ---------------------------- -4----------------------------------------------------------------------------------------------------- Volumes of Effluent and dilution water (Receiving Water/Reconstituted Culture Water) used in preparation of test solutions. Effl uent Conc(%) Effluent (ml) Dilution Water {ml) Total Vol. Prepared (ml) 0% 0 Cioao *4:000 &% 67% 76 70 loot touo XOW 116 0 3 H9o CtOOO xq&o I 910 169-0 1 YX> 960 `o &0 o Signed: , [f P M .1 /n / VVV 000004166 (4o il a. 1 El tHo* UsJ: O XO \ f U5 J la s------ 1 0)35 \ wa> o-M; ik s: to Q% 'i si *i.$1 *3 i Cj r * l < i. /*5:s wd \4_<* Kd > 1 sj>. i* c \M N a Sw < s. 1 ) 1 1 ? c3 ys A . ( *H \ {.r sS 2<--*-- co i;& MD & O __l l E </> ^ . Jd l gr SjJ 4 J s rN4 ' s * rv k 3 ,tS k3 tj N d *| 3i s% ( rsa-> *i * 3 i 4 4 ^ c 3D O ?t g<*>j v: s 1 (f<1 1\ i< 3 N l i2 v, o - o J33 s \1ga t4a \s| f5 ia 1sN D <i*> cj crss K 1 \ii O n t> (S Cr" A2 ^3 M HH4^iHM'PMclll DPhlTPM* ' l M 1 l'M^4H as s H^MH4^I4-W11 <1-H *11M j W11 ` W11' 1 I i 1 y * MdsHVii m 1 VI |^qWo|o|-| 11 \ l f ( ' 'l'H2W2| M s -MNM < M < M M NVbtel i HHa^N^MHsM^HvH^MctH^^N^NoN^^loNsId^ #)gi| TEST CONT. LETTER A HETER NO DATE TIME INITIALS -=i^s)^i c|^sjs( si s) sjsl^l ^ c||-sl ^ acase^ CO tJ O c CO tJO<X. ecu.J Q X CD t_> Q < CO cJ> Q X CO tJ o CO t-> Q CO tJ Q OVCi SC> *4 \n VS SVO t>o o o L14 WV 000004JL67 CONC o Q. ., to o <_> __i1 r^. Q o s3 Id | J) | | H 1 1 1 E J*- 3 1to Q_Oi U3J 4z tan I3T | Qimilh 13 a IH 119 1 0p c> llHl a HI III 1 1 i HUM Ifj i ihl 1 1 4H 1 J\ i 0 n 1i 1 NEW OLD 1 = r:y$ 4 ,) , 1ii ___ 'L. 3 1 tel Ltd 01 Mil 1 1 I miim 11 --o 1 |1 ---"re rl (50 'U a 1z o | 1 LJ CO ] 5| 1 1I1 1 K-j/gjOou>-J1 CMo--oJ 311 r< y 'V& da 1411 N 1 Mil m Mil 31 (3 6^ | p $ a w & vS K 1 |O P 11 A A 8 | N- t* IMI mil 1 Mil H i lid turn ( \ 1 H IM ffl aiiMii 1 Ihl H Bril 9 11R H 3 HIMRim I^J lr^ tt- aim 11 p 1 a H v> 13 0 3 H d 1 Ml m \r - - H h 1h X. 00 <_) O <c m Q <c CO CJ o r CO C_) o c CO C_J o X CO t_3 Q t CO o CO C_3 O x CO o o oz oc czoo_> O $ fc! hVD 1^ LhLSlU--J $8 vvv 00000 *l6y 1-- " test CONT. LETTER ]' o 1' fTJ 1 1 to 1 oUJ- o ] VO aDC; |6C s r0* be tt y zz C3fc-I ol (r 'j- Jr r>s rJ ] $1O_J aM> o co tr ft Ixl z- a er if rj_ LrT o <r o8 W'O^ tto UJ P* C* a: ^ cm 3 | c UJ bo be D_ (o to * 'S ? if O Lx a s be lr /S fas V (f nl 4 a ft r\ U i^ t* S \> lr> A 0>. ft* *> a cl sUJ Lr o kH Xf s. (A c? fN SI <s -M 1 1 H 1 1 13 1 ua 1 1 1st l III 1 \ 1 W>4) bo `tillBMIHIIEIIIiai ) hi l 1 1 13 1 1 M j o H3I 1 Id 1 1 1 lid 1 1 PI 1 1 Id 1 hi 1 1 III 1 1 R 1 1IH U"DJ I a) '-tel 1 1 W 1 1 Id 11 H 1 1 H 1 m 1 A r< m lit* 11 h ny i m 11 m i h 11 m i r 11 m i m i b 1 1 IH J -R 1 IH 1 1 Id 1 1 13 1 1 W 1 *r 1 H 1 1 H 1 1 H 1 1 M -m i w i mn mum lr r< 1 K 1 1 W 1 1 IS 1 1 N LETTER A B C D A B C D A B C D A B C D A B C r\ J co 3 c a3 <_) t23 r cDUC:<cDUC3 <C tE U Q Zo i UOJ' o\o a &o <_> Ch- Pm Its N> r\ O O % ** <b Wv 000041 69 OBSERVATION: SAMPLE WEIGHT DATE: Test Organism: _ Species P. ENSR EL/H Form No.: 005 Revision: 01 Revision Date: 3/89 Source ____________________ Lot No. ffg-f'/7<t Dried in Oven: ___________, Cone (*) Boat Rep # TARE (gm) Control A .Ocffp- P 'Al CSS-L* ' C a r~ Arf\ 7 .-011 a P A .1-' .009/ B ..flnsfr C & .If* D **' / ,(CT1 A B C D A B C D A B C { D A B C D A B C D A B C D A B C D A B C D Initials to GROSS (gm) OfU?7 .run 7 ' , 3 NET (g*n) \Oii)Q .00,2 S' . .M / No of Wt/flsh Fish (mg) 1 1 i L .3/ 3 <<=> b O .3 s o .U^A Notes o.3o VVV 000004170