Document roZvqK7OkX0DR7BbBEKmg53J

Pimephales Summary. ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity:PerfluorooctanesulfonatLei,thiumsalt;may alsobe referredto as PFOS Lisalt,FC-94, or FC-94-X. (1-Octanesulfoniaccid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecaflliutohriou-ms,alt, CAS # 29457-72-5) Remarks: Test sample was taken from 3M productionlot#1. The test sample isa mixtureofthe testsubstance inwater (approximately24.5% testsubstance and 75.5% water).No calculationwsere made to adjustfor the actualconcentrationofthe testsubstanceinthe testsample. METHOD Method: Not noted. Type: Staticacute GLP: No Year completed: 1994 Species: Pimephales promelas Supplier:AquaticBiosystemsInc.F,ortCollinsC,O Analyticalmonitoring:pH and DO content Exposure period:96-hours Statisticamlethods: LC50 valuescalculatebdy Trimmed Spearman - Karber. Test fishage: 79 days. Length and weight: Average length= 2.1+ 0.3cm, Average weight 0.069 + 0.03 g Loading: 0.69 g fish/L Pretreatment: None Test conditions: Dilutionwater: Carbon filterewdellwater Dilutionwater chemistry: pH: 8.4 DO: 8.1 mg/L Stock and testsolution preparation:A primarystocksolution was prepared indilutiownaterto yielda testsample concentration of 400 mg/L. Alltestsolutionswere made by dilutintghe appropdate amount ofstocksolutionwithdilutiownater to make I L ofsolutionper concentration. - Stability of the test chemical solutions: Not noted. Exposure vessels: 2 L glass beakers Number of replicates: two. Number of fish per replicate: ten Numberofconcentratiosnisx:plusa negativceontrol Waterchemistrdyuringthestudy: Dissolveodxygenrange(0- 96 hours): 6.0-7.2mg/L (controelxposure) 4.8-7.9mg/L (56.0mg/L exposure) pH range (0- 96 hours) 8.0-8.4(controelxposure) 8.0-8.4(56.0mg/L exposure) Testtemperaturerange (0- 96 hours) 19.2-19.50C RESULTS Nominal conc entrations:Bk control3,.2,5.6,10.0,18.0,32.0,56.0 mg/L Element value: 96-hour LCso = 19 mg/L (95% C.I.:16-24 mg/L) Mortalityof controls:None Remarks: Values reportedare forthetestsample. No calculations were made to adjustfortheconcentratioonfthetestsubstance inthetest sample. CONCLUSIONS The testsample containing24.5% PerfluorooctanesulfonatLei,thiumsalt exhibiteda 96-hourLC50forfatheadminnow of 19 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O.-Box 33331, St.Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking2. Thisstudy,whilewellconducted,lacks analyticadlata for:determinatioonfthe testsubstanceconcentrationinthe testsolutionsa;nd determinatioonf thesample purity. REFERENCES Thisstudywas conductedby the3M Company, Environmental LaboratoryL,ab Request number M1018, 3/25/94. OTHER Last changed: 5/2/00 e4VIRONMENTAL LABORATORY LR- M1018 Date:3/25/94 Analyst:JBD PiMephates PrOM0188 96-hour ToxlcltyTest Data Summary SAMPLE: FC-94-X (F-I1367,Lot#1) Sample Conc. (mg/L) 3.2 5.6 10 18' 32 56 24 hours 0 0 0 0 0 0 Percent Mortality 48 hours 72 hours 0 0 0 0 0 0 0 10 0 50 20 60 96 hours 0 5 15 so 65 100 24 h LC50 (95% C.I.)= 48 h LC50 (95% C.I.=) 72 h LCSO (95% C.I.)= 96 h LC50 (95% C.I.)= > 56 mg/L > 56 mg/L 36 mg/L (15-85) 19 mg/L (16-24) NOTE: Spearman-Karber method used tocalculattehose LC50s which couldbe estimated. (72 h LC50 required40% trim,96 h LC50 didnotrequireany trim) ENVIRONMENTAL LABORATORY LR rn 1c) 96-HR ACUTE TO)AC[TY TO FATHEAD MINNOW, PIMEPHALES PROMELAS TYPE TEST' sTAmc STATIC RENEWAL TEST SUBSTANCE-. 7 C--q L4- X (v- DILUTION WATER: CARBON-RLTERED WELL WATER (3M WELL #Z ST. PAUL, MN) TEMP. @O-IEOC,PH FA,Lk , D.O. E. Img/L TEST FISH SOURCE.- DATE RECEIVED-. \-Cq L FISH AGE: CA "G. Vff.:C).ek(k :t.0-6 "G. SIZE: cm NO. OF FISHrrEST CHAMBER-. t0 LOADING RAM- tL so\,c.\ TEST SOLUTION VOLUME: L SIZE OF TEST VESSELS: L DATE INITIATED: Z-a\-CiLi,Cet 1( 00 DATE TF.RMINATED@. TEST INITIAL TOTAL VESSEL CONC. AMOUNI mWI ADDED* SKI -CONTROL K2 CONTROL 24-HR PH D.O. mgAL R. "A I (C MORTALITY 48-HR pH D.O. 72-HR pH mg/L I IIli,. (0.(0 C> 1 9@. LA C) ik, -:@ D.O. WHR mgk -I. C) (,$,-I C) pH I MDW.LO. 0 1 -a -c-k 2 $I 1 s-,r4, 2 0 LA C> S@LA 6.9 I Co. CA 0 p C> C) oo@'3 (o.C4 C) 2 It 0 19 2 1 a7@L@o 2 &I 0 tk4S 0 (co,-.7 C) C) $,I pit C) co.ClI c> c&, -7.Z LA 41 m -r<-.A -I.aL I %%To C) 1%.ko 0 S.-S 2 #A !S S co let \C) LA I (01-1 ct 2 2. TEMP. OC DATE / ANALYST I-QtZL-Ci%-k LCS(O95C%.I.) Q(9V\ 4TE7@:> C=i I z- CI'N-Ci%-k I Z-Zk ici L. ((o-aLk .n c, COMMENTS @m k Jt @ r M5 1L. k-@r t-.CN w,\V\ cr, LLN c- o t-a ACT\ f-t! 4S(P,C, m S, L q'@ 0'%;@@-A :Mvencvr.An- kle. (no r v.@Lm) SECONDARY ER-FECTS: S SurtaftE Lm ofEqullbdm;0 Od"con% R ErradcSwimffdn H Hemorrhoong ANALYST. !O(M1060-8-D- PWO 3ample Description v- C- Ck Y.- 7-- -INN ;L LA CD EnvironmentalLab - Work Sheet Lab Request No. MtC)kZ Page- of Date: Ct L-t Analyst-:;T-Zb C\ o- 0, C.@ cc 'Y\ @4-co 14 Lis %I \so'.\ %A'@;@. Iro rESEARC!-4. TRIIIMED SF-EARMAN-KARBER METHOD FOR CALCULATION OF iEC50 AND L@--5C@'Y'ALUES IN L;TGASSAYS FOR REFE-%'ENCr-_. CITE . M.A. HAMILTON, R-r-'. RUSSO. ^4ND. R..V. THURS7mN. 1977. TRIMMED SPEARMAN-KARB-MR M"ETHOD FOR ES"FT*IMATING MEDIAN .LETHAL CONICENTRATIONS IN TOXICITY B-GAS--2AYS. ENVIRON. SCI. TEC.-4NinaL.14@(7) 74L4-719 CORREI--"I-ION12(4) 41-ir (1@;76%1. DAT,-=- -7iEst-rf..f. @".HEMrLOAL - mioia FC-F-4-"' DURA-,R I C)N r a inL= --si 9 i-,-nours 9,AW DATA CCN'-ENTRA710N(mc,*@%.. :_I NUMBER EXPOSED MORTALITIES SPEARTIAN-KARBER TRIM . 3.20 'li.20 0 5.60 4 C) 1 10..00 2o :3 '0.()C) is.OCI 4-C) 10 :32.i)O 56. 1--:W@e. 20 i:z 20 SPEARMAN-KARBER ESTIMATES L050 19.46662a4 95% LOWER CONFIDENCE .15.6:i 95% UPPER CONF-LDENCE 24.00 ------------- ------------------------------------------------------------------- BURLINGTON RESEARCH. INC. Tri-mmed Soearman-Karber Method +or Calculation a-F EC50 and LC50 Values in Bioassavs 4 2,rE-:. x =@2E E 2.5i5E .3 x 3 .3 .3 ix 3 x x x 1.006E .3 .547)3 E .3 GOODDDEDDDODDDDEDUDDDDDDDDDDVDED.DDDDDDDVDDDDDEDDDDDDVDDDDDDOE LC L025 LC50 LC75 MORTALITIES LCIOO TEST miois CHEMICAL SPECIES 3:.;RATION RAW DAT(.@ FC-94-X P. oromelas 72 hours ,@.CNCENTRATION(ma/L) NUMBER EXPOSED MORTALITIES SPEARMPAIN-KARBER (-RIM 3.20 20 .0 5.60 10.0(.11, 20 20 0' 0 40.(')0 16.00 2() 2 Z7,2.00 20 10 56.(-.)(-) 20 12 SPEARMAt\l-.KARBER JESTIMATES LCO---O 35.5052032 95% LOWER CONFIDENCE 14.e6 -----------9-5-% --U-PP-E-R---CO-N-FI-D-E-NK---,E-------------@-4.-,-3-5 7 -------------------- IVA LA % BURLINGTON RESr@-ARCH. INC. Tr4;.mmed Soearman-Karber Method for C,:Elcu@Lation of EC50 and LC50 Values in Bioassays x 3 x 3 6.044E 3 S. Ief)E x L 4.:7,17E .3 3 :5.45:7-E .3 3 T .-I 2.590E N-@ -.3 1.726E .3 3 86Z-.4E 3 .'3 ODDODDEDDDDDDDVC-PU"DDDDDDDDVDDDEDDDDVDDDDDDDDDEDDDVDDDDODODDDE LCIC) L f-2- LC50 MORTALITIES LC75 LCloo