Document rex5d9xROM2eg5Gm0vd8870qG

AR 226 - 0838 ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TESTSUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8- heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was not noted. The test sample is FC-600 (Belgium AFFF). Current information indicates itis a mixture of 1.0% PFOS, 81.20% water, 12.00% diethylene glycol butyl ether, 1.00% sodium octyl sulfate, 2.00% propane Sultone foamer, 1.00% sodium decyl sulfate, 0.85% xanthan gum, 0.1% N-(3-chloroallyl) hhexaminium chloride, 0.80% starch, and 0.05 % tolyltriazole. The following summary applies to a mixture with incompletely characterized concentration of impurities. Data may not accurately reflect the toxicity of the fluorochemical component of the test `sample. M -- EO --C-- G} ------ Method: Procedures closely followed those outlined in EPA-560/6-82- 002, OECD, and ASTM Proposed standard practices for conducting acute toxicity tests with fish, macroinvertebrates and amphibians, E729-80. Test type: Acute flow-through GLP: No Year Completed: 1982 (study) and 1983 (report) Species: :Pimephales promelas Supplier: Commercial supplier in Brady, Nebraska. Analytical monitoring: Temperature, pH, and DO Exposure period: 96-hours Statistical methods: LCs values and 95% confidence intervals could not be calculated. There was no mortality during the test. - Test fish age: Juveniles Average Length and weight: Length = 3.8 + 0.45 cm Weight =0.42 +0.11g Loading: 0.47 g/L. Pretreatment: Not noted. Test conditions: Dilution water: Carbon-filtered well water, 3M well #2, St. Paul, MN. Dilution water chemistry: Dissolved Oxygen: pH: Hardness (as CaCO): Alkalinity (as CaCOs): 95 -- 98% saturation 79-81 250 mg/L. 230 mg/L 002057 Conductivity: 340 pmhos/cm Lighting: Cool white fluorescent lights provided a daily photoperiod of 16 hours light and 8 hours dark with a 30 minute transition period was maintained during testing. Intensity was measured at 60 -- 70 foot-candles at the water surface. Stock and test solution preparation: A 40,000 mg/L stock solution was prepared daily by diluting 240 gramsof test sample to 6-L with dilution water. Test solutions were prepared by a diluter delivering five nominal concentration of the test sample and control water. Flow-through rate: Approximately five volume replacements per 24 hour period. Stability of the test chemical solution: Not noted. Exposure vessels: 24 cm diameter by 25 cm deep cylindrical glass aNquumarbiearwoitfhraepmlaiicnatteasi:netdwotest solution volume of9- liters. Number of fish per replicate: 10 WNautmebrecrhoefmicsotnrcyendturraitnigontsh:e 5stpulduys:a blank control PH range (0-96 hours): 7.58.2 (control exposure) 7.58.2 (1,000 mg/L exposure) Temperature range (0-96 hours): Dissol1v8ed-- o2x0ygCe(nforr aalnlgaequ(a0r-i9a6)hours): 6.7 -- 9.0 mg/L (control exposure) 6.1-9.0 mg/L (1,000 mg/L exposure) RESOLYS 0000000000000 Nominal concentrations: Bkcontrol,100, 280, 320, 560, and 1,000 mg/L Percent mortality (based on 20 fish per concentration) |cottRe ewr r| Normal [Convo [0[0[0[0 | [0 10 010 0 [20 ToToTo10 | [=To 9% TTo [0 [0 | 010 |T 0 | [foo To10 1oT 0 Element value: 24-hour LCso: >1,000 mg/L oo 48-hour LCso: >1,000 mg/L. 72-hour LCso: 96-hour LCso: >1,000 mg/L. >1,000 mg/L Element values based on nominal concentrations. 002058 Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The value reported applies to that mixture and not the fluorochemical proportion alone. No secondary effects were observed during the 96-hour flow through test. C0 ONCl0 usi0 ons0000000000 T>1h,e00F0C-m6g0/L0.96-hour LCso for Pimephales promelas was determined to be Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 b0 ara0 QuAL0 TY 0000000000 Reliability: Kiimisch ranking 2. The study meets all criteria for quality testing. However, the sample purity was not property characterized and the study lacks analytical confirmation of the amountoffluorochemical proportion in the solutions. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, 3M Lab Request number 9595, 1982. 3M Technical Report "Aquatic Toxicity Testing: FC-600 (Belgium-AFFF) LR 9595." M. T. Elnabarawy, Project 9970012600, Commercial Chemicals, Report Number 82, July 21, 1983 oTHER Last changed: 6/28/00 002059 = Fomerarine TECHNICAL REPORT SUMMARY TO: TECHNICAL COMMUNICATIONS CENTER -- 201.2012 (Important -- 8report sprinted on both sides ofpaper,snd two copes to TCC.) Guidelinoens revere sic. Environmental Laboratory (EE & PC) Comercial Chemicals Aquatic Toxicity Testing: FC-600 (Belgium-AFFF) LR 9595 SUEALReiner sy M. T. Elnabarawy 0535 9970012600 gz 7/21/83 46981 secunirep Doonan Dinerosary| SAGIEHEN pOriceue wees ome Kepwerts E(EEnv&. PCL-aDbi)v. CTURoREdNeTtGeRrECmTiInVEe the acute aquatic LC), following exposure t vtharroyuignhg ccoonndcietnitornast.ions of FC-600 {Belgiun-AFFF) under flc T(oAxqiucaititcy) EREEPORST ABSTRACT: Th shat rmaion + GHed oe TodvweCommtesion Cor Her 1 mThiennoawcsute(Paiqmueapthiacles96p-rhoomuerlaIsC)g',fovlallouweisngobteaxipnoseudrefortofaFtCh-e6s( uinndrereplfilcoawt-etdhreoxupgehricmoenndtitsiownesr.e gTrheeatearcuttehantox1i,0c0i0tymgo/fl FunCd-e6r00thteo rageupaotritcedotregsatniscmosndwiatsioncso.nsidered insignificant nigoernman Listotenn 002060 ACUTE TOXICITY OF FC-600 (Belgium-AFEF) FATHEAD MINNOW (Pimephales promelas) UNDER FLOW-THROUGH CONDITIONS LR No. 9595 (9970012600) Prepared by: MS.r.T.EcEoltnoaxbiacroalwoygist EEEnvi&roPCnmental Laboratory 3M Company - 2-38 July 20, 1983 002061 INTRODUCTION T(hBeelpguirupmos-eAFoFfF)thitso sftautdhyeadwasmintnoowest(iPmiamteephatlheesapcruotmeeltaosx)iciutnyderof flFoCw--600 sthurbosuegqhuenctond9i6t-ihoonusr. flPorwe-ltihmrionuagrhy tersatngewe-rfeindcionngducstteadticat tetshets and a EtonxviicriotnymenotfalFC-L6a0b0orawatsorym,eas2u-r3Ee,d Sbty. esPtauilm,atiMnignnetshoetaL.CS0Thvealuaecutfeor the tchoempocuonndcenattraetaicohn 24ofhotuhre tiensttervcaolmpooufndtheintedsitl.utioTnhewaLtCeS0r wihsicdheficnaeudsedas mionrttearvlailt.y ofThe50%floofw-tthheroutgehst teasntimawlaspocpounldautcitoend aftrotmheJulsytat1e1d-15e,xpo1s9u83r.e TEhlenadbaatraawyrepaondrteR.d Rh.ereRionbidaeraeu.baseAdllonrawstdudaiteas gpeenrefroartmeedd abrye M. T. stored at the above location. MATERIALS AND METHODS - dPersoccreidbuerdes inusetdhe inU.St.hisEPAacuptreototcooxlicietnytittleesdt c"lEonsveilryonmfeonltlaolwedEfftehcotsse Test Gpeuritdienleinntes"are(19l8i2s)t.ed Oitnhetrhetersetferpernocteocoslescticoonn:sideArSeTdM-Ep7a2r9tic(u1l9a8r0l),y OECD d(e1p9e81n)d.ingVaolnuetshearaeccurreapcoyrteodf ttohedmieffaesruernitngldeevevliscesof isnivgonlivfeidcainnceany one process. T1h5,e 1s9a8m2p.el oTfheFCt-e6s0t0,mataerviiaslcouwsasamsbteorredliqiunidt,hewdasarkrecaetivaemdbieonntSeptember tNeommpienraalturetes(t21cCo)n.ceTnhteramtaitoenrsiaalrewarsepotretsetdedasasmi1l0l0%igraacmtsiveof iFnCg-r6ed0i0enpte.r liter of test solution (mg/l). Tchoemmefractihaelad fmiisnhnoswusppl(iEenvr. inLabBraLdoyt, 2-N1e9b8r3a)skaw,ereanodbthaeilndedinfraom47a5-1 dfairbkenregslsasswitthanka u3n0d-emrinu.atephottroapnesriitoiodnofper1i6odh.oursAllligfhitshanwdere8 hfoedursa basic danrdieddurfiinsgh ftoeosdt,ingT.etraThMeirne, wadsaillyessexctehpatn d3u%rimnogrtatlheity48 inhoutrhse ptreisotr fitosh pthoepulhaotlidoinngdutrainkngwatshis20+21-dCayduarcicnlgimatthiiosn2p-edraiyodp.eriTohde (tDeamilpyeraRteucroerdinof wFaisshcHhoalrdaicntgerCioznedditaisonhsa)v.ingThteotwalellhawradtneesrswhaincdhalfklaowleidnitiyntorantgheiss atasnk cspaelcciifuimccacrobnodnuacttaenc(eCaCo0f;>)340ofmi2c5r0ommhgo/sl paenrd 2c3e0ntmigm/elt,erre(supmehcotsi/cvme)ly, and a (inMontthehlyholWdaitnegr QtuanaklitwyereAnaalypsHisraLnoggebooofk)7..9-8O.t1h,erapadriasmseotlevresd mooxnyigtenore(dDO) vroalnguemeofrep95l-a9c8e%menofts/sdaatyura(tDiaoinlyanRdecoardfloofw rFaitseh oHfoladinmginiCmounmditoifon2s)t.ank dTaeysst. fiTshhewersepemcaiifnitcaicnoendducutndaenrcetwhaessemecaosnudirteidonwsithfora YaSIminMiodmeulm o#f31 14 cmeotnedructainvditcyo-mbibnraitdgieo,n etlheectprHodwea,s mtehaesuDrOedwawsitmheasanureOdriownithModaelYSI407MAodeplH w#i54thdiasnsoAlSvTeMd-Caolxyigbernatmeedtetrhearnmdomeptreorb.e aTndotatlhehatredmnpeesrsatuarned waalskamleiansiutyred were measured according to APHA et al. (1980). 002062 PCa=ge6002 July 22, 1983 TGhleasstogxaiscandtri-vdeenlivseirpyhonsysttyepem),usepdropionrttihoinsalstuddiyluwtaesr asimmoidliafrietdo, th(aAtCE dfeasctcorri.bedThbey MboiuonatssaaynddBirluuntgesr w(a1s967c)onwsittrhuctaed0.0p5rimianriitliaylofdiglluatsiso,n #316 wSattaeirnlewsass Swteelell,wataenrd wpheircfhluowraoscaprubmopnedpltaostaicpsoly(eTtehfylolne)n.e cTohnestandti-lhuetaidon reservoir box characterized wahserheaviintgwatsotaalerahtaerddnewsisth anfdiltaelrkeadlinaiitr.y raTnhgiesswaasterCaCwOa,s osfpec2i5f0icmg/c1ondauncdta2n3c0e mogf/l,340reuspmehcotsi/vcemly(,Mona thplHy raWnagteer ofQua7l.i9t-y8.1Anaanldysias wLeolglboowka)t.er Aat.ctohmepleEtnevirl.istLaobf fatchielipthyy,sic2a-l3/chiesmiactatlachcehda.racteristics of TchoencebnitoraastsaiyonsdiloufteFrC-6w0a0s;ca1l0i0b0r,at5e6d0, to320d,eli1v80e,r 1f0i0vemgn/olminaanld cloongtarroilthmic wgaltaesrs ctoesnttaianqiunagriunom mFeCa-s6u00redto24dupclmiciantediatmesetteraquaanrdia2.5 cmEadcehepcywliitnhdri2c1al dcemlihvieghreddra0i.2ns1whoifchtemstainwtaatienredtoweaatcehr avoqluuamreisumofat9an1. aveThreagedilruatteerof r22e5placgyecmleesntsperpedray2.4-hTohuirspeirsioedq.uivaIllelnutmintaoti5onaqwuaasripumrovviodleudmeby Cool Wthhietew"atefrluosruersfcaecnetealcihghtdsay.forTe1s6thotuermspemruaetausrueriwnags 6c0o-n7t0inufoouostlcyandles at muosninigtoraedTayilnoroneteOmfpertaheturceontcrhoalrt-traenckosrddeurr.ing the 96-hr exposure test Asol1u0-t1ionpoltyoetthhyelemniexinbgottclheamwbaesr ouftiltihezeddiltuotedreliwvietrh etvheerytocxyicclaen.t stThoeck FsCt-o6c0k0soalnudtidoinlutofing40t,o0006,m0g0/0lmlw.as pAretpoatraeldamdoauinlty boyf a96d0dinggram2s40ogfraFmCs-60o0f was used during the continuous flow study. AtfhteerFCe-s6t00ablsitsohcikngsotlhuetiodnesiwarseditnetsrtoduccoendcenitnrtaotitohnesdiinlutteher atnesdtcayqculairniga, mbeignannowsonuJnuilfyorm11,in1s98i3z.e anTdheagteeswtitwhasa imneiatniat(eNd=wh1e0)n wteetn wfeaitghhetadand. tLoetnaglthslenLoggt)h owfere0.4r2a+n0d.o1m1lygrdaimsstriabnudte3d.8+t0o.e4a5chcmaq(uFairsihumW.eighAtstotaanld of 20 woarsganciaslmcsulawteerde etxopobseed0.4t7o geacfhishconpceerntlriatteirono.f teTshte sfioslhutiloona.ding ratio Ntoheaetresatt.ion of test solutions in all test chambers was provided during TmheeasuprH,edteamtpe0r,at2u4r,e48a,nd7d2,issaonldve9d6 ohxoyurgsenin(DOb)othconrceepnltircaatteisonsofwearlel cofonctehnetrfaitsihonisncliundcilnugdinmgorttahloisetyofandtheseccoonntdraorly. effBeicotlsogi(cbaelhaovbiosrearlvations observations) were made and recorded daily. Tthweicegendearially otpherroautgihoonutofthethetesbti.oassay diluter was checked at least 002063 FPCa-ge6003 July 22, 1983 Tfhreomcotnhecenttorxaitciiotnys tetsetstewdereandusetdhetocoersrteismpaotnedin2g4-,mor4t8-a,lit7y2-,dataandde9r6i-vheodur mceadlicaunlatlieotnhalselceocntceedntriastidoentserm(iLnCeSd).by Wthheencahpaprlaicctaebrlies,tictsheofmetthhoeddaotfa mboarsteal(iit.ye.,ofpr1e0s0e%ncoef otrheabtseesntceanoifmaltesptopucloantcieonnt,rattieosntsccoanucseinntgrations scatues)i.ng mortality of a partial number of animals in the population, RESULTS Tnhoemintaelst cmoantceernitarlatiFoCn-s600as wahsighnotas ac1u,t00e0lymgt/olxic(Ttaoblefat1h).ead NmoinFnCo-w6s00 at r96elahtoeudr meoxrptoasluirteypeofriofdi.sh Texhpeoseexdposteod1,f0i0s0h iagp/p1earweads ogbesneerrvaeldlydhureianlgthythe aofndexapcotisvuer.e weTrhee e>s1t,i00m0atemdg/lL,CS5t0hevalhuiegshesatftecronc2e4,ntr4a8,tio7n2, teasntded9.6 hours Ntohecotnesttr.ol organisms died or showed signs of disease or stress during Tahned dtiedmpenroattuvraeryinmorvearitohuasn a2gCuariinaanryang1e2d-hofurrompe1r9i-o2d1.C dTuhreinpgi eanxdposure, dpirsessoelnvteedd oixnygAeqnuatciocncTeonxtircaittiyonsDamteaasSuhreeedtsdu(raitntgachtehde).toxTihceitypHtelsetvelasre driedmainnoted fraelllatbievleolwy60u%nchsaantguerda,tioann.d the dissolved oxygen concentrations Tahceuterleysulttosxicleatdo ttohe thaequactoinccluesnivoinrontmheanttFaCs-60a0re(sBuelltgiuomf-AeFxFpFo)suries unontder comparable conditions. REFERENCES AaPnHdA,waAsWtWeAw,ateWrP.CF. 15t1h980E.ditiSotna,ndaWrasdhimnegtthoond,s Df.oCr.,the1e1x34amipnp.ation of w-ater TAoSxTiMc-iSttyandTaerstdsPwriatchticFei,she1s9,80.MacrSotiannvdearrtdebPrraatcetsi,ceanfdorAmCpohnidbuicatnisn.g Acute Am7e29r-i8c0a.n Society for Testing and Materials, Philadelphia, PA, ASTH- cLeomnkset,rucA.tioEn., anW.d Ao.perBartuinogns oafndtBo.xiJc.ityH-atlleisgtainn.g pr1o9p78o.rtioMnaanlualdilfuotrers. EPA-600/3-78-072. Mfoourntf,ishD. toI.xicaondlogW.y As.tudBireusn.gs. Wat1e96r7.Res.A sl:imp2l0-i2f9i.ed dosing apparatus EOEcCoDn.omic198C1o.operGautiidoenlinaensd DfoervelToepsmteinntg, ofWasChhienmgitcoanl,s. D.CO.rganization for U.S. EPA. 1982. Environmental Effects Test Guidelines. EPA 560/6- 82-002. 002064 . Table 1. Cmoorntcaelnittriaetsionosf tfeasttheeda,d mcionrnroewspo(nPdiimnegphapleerscentage Bornodmietliaso)ns:expo"tsweedntytofFisCh-60w0ereundeexrposfeldow-tethreoauchgh concentration. ConcNeonmtirnaatlion (mg/1) 1000 560 320 180 100 Control Leg, (mg/1) Ti Four 0 0 0 To o 1,000 4P8ercehonutrage72Morthoaulrit15% hour -- 0 0 0 0 0 0 0 o 0 To 0 0 0 0 0 0 0 >1,000 51,000 >1,000 002065 VeMe2. WELL WATER QUALITY SAMPLE DATE: 6/25/83 SAMPLE TAKEN: 3M Bldg. 2-3, St. Paul SAMPLE DATE 6/25/83 (TAKEN FROM ROOM 5) PARAMETER . pRHesidual Ch5lorine ACmomnodnuicativNiittyrogen M-Alkalinity TToottaall HSaorldindesss TDoitlaulte FClOuDoride TSottaanldarCdoliPflaotrem Count AArlsuemniincumasasAsA*l* BCoobraolntasasB*Co* CCohprpoemriumasasCu*Cr? CIarodnmiuasm Faes*_ca* LNeiacdkelasasPb*Ni* zSiinlcverasaszn*Ag* Mercury as Hg* PcB's TotalPeOsrtgiacniodcehslorine TotalPeOsrtgiacniodpehsosphorus *sample Date 3/10/83 CONCENTRATION 07..902 mg/1 <3400.1umngh/o1s/casm Nat 25C 223800 mngg//11 as CaCO, 205.002mg/m1g/1as Caco, <N0e.g4atimvge/1 0 per m1 <1 19ug/ulg/1 1<55 uugg//1l <<23 uugg//1l 1<10.1ug/u1g'/1 <<17 uugg//1l 8<95 uugg//l1 0.7 ug/1 : <0.01 ug/1 0.01 ug/l, <0.01 ug/1 WATEENVR. QLUAABLITY BOOK REF. 85 89 88 89 109 10 1166 1166 1166 1165 1166 116s 16 17 17 1 002066 ENVIRONMENTAL LABORATORY DEeIITIVE (FULL-SCALE REED ia ASES poeLoi2 Te es huts,AreKou Haugntaterial Tested ete Fume(Beau) 1060-00 0 .O (4r 7prre ens Ae vgW. iSn inzen5i SBoucrS mceNBoi .eOargtm aneisNme selperDn adotsee_f/e()c'ldoa_d2i/n7g/gFa2c)torBJatceh 1t2s-8a5n ih Disease Treatment: _ AUF SE eVEre Te Pere TR st Solution Volume2. Q_Liters/AllGlass stareed_4/:00 flowin sects Tank Tank Dimensions: {gE ove 35x20 cn corviecss_t and 25 cm deep s/s tion Water Carbon-filtered well water (Tero. _/ _C., 0.0._Z, 0) pom, pi_ 2 ) pp a si a- toy, (oC Btidence Linits) >/000 sly re >. | one" | amount [Totaal veo.Josa [o.0. [rotawl Neo.Jsot [oo rorrtoo. w [eto fromtSono]ws loa: CL Tr Tr rr1 LT 17J1 r 1] Hnerp, oe | et e _ te1 nil2 Zioaec.e0 e|apt ZR lC of e q|TE7TC5f9 [ o Ee e r e ee Ccondary Effects (other than Mortality): SET Var EC50_ Ter B - am sem. OPY AVAILABLI loz KSyd | | 77 | | OZ Sl ADEQFUIANTIITCIVTEOXI(CFIUTLYL-DSACTAALESTHEESETT) R_LSZSpace 208 = i OTSrGaetntySAtarreeHnhoswurmaiigg)upoetweseiaB l Taets Ay AtEv, uae atend v(eFdro ,gu(mh 2)/i7oi /t8p 2ieo at veno- v 2-85 3Wt. 2HZ)grans avg. Size 5, B on No. Organisms per dose_/() Loadingractor_ONp an st SolutionVolume _Liters/All-Glass Tank Tank Dimensions: 35x20 an and 25 om deep Started dorm Date Started ABs Date Completed sles wegfe 275 ution Water Carbon-filtered well water (Temp. /8 C., D.O. 20 Loy, (95% Bonitence Limits) pen, pH .2 ) EEE ETE T TE Fe x Jenieia [moms fe20 fours ~ eee 48 fours ~ 72 fours -- we 96 Hours -- L2edNE |I 0 CTT T7odealI 0 ETT 7LiedWPo b7la TTT EF14 F eof ZE0K oO fe) ote Ye2d ZLdo a Te LC Tor tar alT = r delsI tT d.[ T0 11d bie ga eeralste tay i Hin--om |Zoe c.eR zooO .|2z0or0. d L2O0CC. -------------------------- -- te ole re e pa e e"e 11] I 1 1 | 11] Te