Document rek0N6k505J46gVKp8krz7ymv

Wildlife International, Ltd. "1" ProiectNumber454A-108 PFOS: AN EARLY LIFE-STAGE TOXICITY TEST WITH THE FATHEAD MINNOW (Pimephales promelas) ADDENDUM WILDLIFE INTERNATIONAL, LTD. PROJECT NUMBER: 454A- 108 3M LAB REQUEST NO. U2723 U,S, Environmental Protection Agency Series 850 - Ecological Effects Test Guidelines OPPTS Number 850.1400 and OECD Guideline 210 This study was reported using a test substance purity of 90.49%. The test substance characterization was revised on 9/7/00 and the purity was determined to be 86.9%. The Sponsor requested that the results be revised based on the most recent purity analyses, The results previously presented were simply corrected by the ratio of the two purities or a factor of 0.9603. No attempt was made to recalculate analytical standard concentrations or measured concentrations from original raw data, As such, minor errors due to rounding may be present. Attached is the revised summary page from the final report and the most recent certificate of analysis. Wildlife International, Ltd. "2- P,ojo tNumborn54 REVISED STUDY SUMMARY BASED ON A PURITY OF 86.9% SPONSOR: ,v 3M Corporation SPONSOR'S REPRESENTATIVE: Susan A. Beach LOCATION OF STUDY, RAW DATAAND A COPY OF THE FINALREPORT: Wildlife International Ltd. ,. Easton, Maryland 21601 WILDLIFEINTERNATIONAL LTD. PROJECT NUMBER: 454A-108 TEST SUBSTANCE: STUDY: NOMINAL TEST CONCENTRATIONS: MEAN MEASURED TEST CONCENTRATIONS: PFOS (Perfluorooctane Sulfonic Acid Potassium Salt) PFOS: An Early Life-Stage Toxicity Test with the Fathead Minnow (Pimephales promelas) Negative Control, 0.13, 0.28, 0.55, 1.1, 2.2 and 4.4 mg a.i./L Negative Control, 0.14, 0.29, 0.58, 1.2, 2.3 and 4.4 mg a.i./L TEST DATES: Experimental Start - August 5, 1999 Biological Termination - September 21, 1999 Experimental Termination- September 22, 1999 LENGTH OF TEST: 47 Days , TEST ORGANISM: SOURCEOF TEST ORGANISMS: Fathead Minnow (Pimephales promelas) Wildlife International Ltd. Cultures Easton, Maryland 21601 AGE OF TEST ORGANISMS: Eggs <24 hours old at test initiation NO-OBSER'$ED-EFFECTCONCENTRATION: LOWEST-OBSERVED-EFFECTCONCENTRATION: MAXIMUM ACCEPTABLE TOXICANTCONCENTRATION: 0.29 mg a.i./L 0.58 nag a.i./L 0.41 mg a.i./L '....... ,,,r ,i .....,,,, Wildlife International, Ltd. -3- CERTIFICATE OF ANALYSIS Proiect Number 454A-108 INTERIM CERTIFICATE OF ANAL YSIS Centre Revision 1(9/7/00) Analytical Laboratories COA Reference 3M Product: PFOS, Lot 217 Reference #: SD-018 , #: 023-018A Test Name Purity1 " Purity: 86.9% Specifications _:d:d.:_:i:4/i/_:?:_:.:_#:!:!i_.ii!i:!:i:!i_:_:::zi:?idi:::_::iF.::::iiiii:dX__:!:-i:i::/:.F.!d!i:il?.li:dF:.!:!_ii:!i_::f!:;!::.i.i.:{id._l._i.:.!._iF.::q!!!ill Appearance Identification NMK White Crystalline Powder _==::=.=::=.=:=:=:=:=:.=:=:=.=.=.=.=.=.=.=.=.=..=.=.=.=.=.=.:=.;;.=:;.=::.=:.:=..:=:.;=:.:=:.:_.=:.=.=.=:.=.=:.::.:.::.::.::.:.::.::.::.::.:.::.::.::.::.:.::.::.::.:.::.::.::.................:.:_..:..:..:.:z: _::='::"' ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: x.:::--:;: Result 86.9% Conforms Positive 1. Calcium 2. Magnesium 3. Sodium 4. Potassium" 5. Nickel 6. Iron 7. Manganese o.%, _np_ Total % Impurity 0NMR) Total % Impurity (GC/MS) RelatedCompoundsPOAA Residual Solvents (TGA) Purity by DSC 1. 0.005wt./wt.% i!!ii!ililiiiiiii 2. 0.001wt./wt.% 3. 1.439 wt.Jwt.% 4. 6.849wt./wt.% 5. <0.001 wt./wt.% 6. 0.005 wt./v,_.% 7. <0.001wt./wt.% 1.93 wt./wt.% iiiiiliii:iii}i;!ii!liii:iii!i!iii!!lii!i!i!iii!i:il_lii _4__,_ None Detected :......::::::....:.........:.:..::..:.....:........_..-..,.. ............................................ 0.33 wt./wt.% _::._:_:_:_:::_..:.._._.:.:-.:.:.-......:.:.:.:.::.:::::....::.:..:.:.:.:.:.:.:.:.:.:.:.::.,-u-_,,.,::::;:_ None Detected Not Applicable 3 1. Chloride 2. Fluoride 3. Bromide 4,. 67. PShuolfsapthea4te 1. <0.015wt./wt.% 2. 0.59 wt./wt.% 3. <0.040wtJwt.% iiii;li:iiiii!ii!iii!iii!iiiiiliii!i!iiiiii4iii.i,i.i{ i ii:!ii:ii.i_ii!i:i!i"_i!i!.ii:i'_,: i 67. <08,70607wwt./tw./wt.%t.% Orga1n.icTAFcAids s (IC) , iiiiill iiiiiii iI il iiiii ii i ;ii i i ii i{!iIl iii i :l 1 <0.1wt./wt.% 4. _PA Elemental Analysis6: 1. Carbon 2. Hydrogen 3. Nitrogen 4. Sulfur 5. Fluorine 1. Theoretical Value = 17.8% 2. Theoretical Value = 0% 3. Theoretical Value = 0% 4. Theoretical Value = 5.95% 5. Theoretical Value = 60% 4. 0.28wtJwt.% 1. 12.48 wtJwt.% 2. 0.244 wt./wt.% 3. 1.74 wt./wt.% 4. 8.84 wt./wt.% 5. 54.1 wt./wt.% COA023-018A Page 1 of 3 INTERIM CERTIFICATE OF ANAL YSIS Centre Analytical Laboratories COA Reference #: 023-018A Date of Last Analysis: 08/31/00 Expiration Date: 08/31/01 Storage Conditions: Frozen [:]-10C Re-assessment Date: 08/31/01 _Purity = 100% - (sum of metal impurities, 1.45% +LC/MS impurities, 8.4 l%+Inorganic Fluoride, 0.59%+NMR impurities, 1.93%+organic acid impurities, 0.38%+POAA, 0.33%) Total impurity from all tests = 13.09% Purity = 100% - 13.09% = 86.9% 2potassium is expected in this salt form and is therefore not considered an impurity. 3Purity by DSC is generally not applicable to materials of low purity. No endotherm was observed for this sample. 4Sulfur in the sample appears to be converted to SO4 and hence detected using the inorganic anion method conditions. The anion result agrees well with the sulfur determination in the elemental analysis, lending confidence to this interpretation. Based on the results, the SO4 is not considered an impurity. 5TFA HFBA NFPA PFPA Trifluoroacetic acid Heptafluorobutyric acid Nonofluoropentanoic acid Pentafluoropropanoic acid 6Theoretical value calculations based on the empirical fon'nula, Cff17SO3"K This work was conducted under EPA Good Laboratory Practice Standards (40 CFR 160). COA023-018A Page 2 of 3 INTERIM CERTIFICATE OF ANAL YSIS Centre Analytical Laboratories COA Reference #: 023-018A LC/MS Purity Profile: Impurity C4 C5 C6 C7 Total wt./wt. % 1.22 1.33 4.72 1.14 8.41 Note: The C4 and C6 values were calculated using the C4 and C6 standard calibration curves, respectively. The C5 value was calculated using the average response factors from the C4 and C6 standard curves. Likewise, the C7 value was calculated using the average response factors from the C6 and C8 standard curves. Prepared By: David S. Bell Date Scientist, Centre Analytical Laboratories Reviewed By: John Flaherty Date Laboratory Manager, Centre Analytical Laboratories COA023-018A o Page3 of3 I tctt zfe Internatzonal Ltd. 1 ECOTOXICOLOGY & ANALYTICAL TESTING SERVICES September 27, 2000 Ms. Susan A. Beach 3M Corporation Environmental Laboratory 935 Bush Avenue 3M Building 2-3E-09 P.O. Box 33331 St. Paul, Minnesota 55133 Dear Susan: Enclosed please fred Addendums for Wildlife International, Ltd. Project Numbers 454A-101, 454A-102, 454A-103A, 54A-104, 454A-105, 454A-106, 454A- 107, 454A-108 and 454A-109 If you have any questions, please let me know. KKD/cywS/3m Enclosures '-.. . Kurt R. Drottar Senior Biologist 8598 Commerce Drive - Easton. Maryland 21601 Tel.410-822-8600 Fax 410-822-0632 E-mail ecotox@wildlifeintemational.com European Of_ce: Overscheenseweg IIA - i412AA Naarden -The Netherlands "Tel.31(0)356949261 - Fax 31(0)356948229 E-mailaleopold_._corldonline.nl