Document reg6eeDrM8xmdbawM2B5mXqQ7
Toxicological Summary
PFOS
Dictary Chronic Plot Reproductive Study: Mallard TestSubstance: Perforoocincsul(fPoFnOaSe)
Structure:
est Remarks:
potion salt CASH 2795.39 1-Octanesulfonic acid, 1,1,2.2,3,3,4,4,5,5,6,6,7,7.8,88-heptadecafluoroTShoereestdsebssiaamnbcieesnacownhdiittiopnosw,daenrd(pBuMttLyowtas#2d17e)t.rTmihneesdamtopl6ew8a6s.9%
by LC/MS, "H-aN ndeMlemRent,al analysis techniques.
METHODS
Method:ASTMStandardE1062-86andFIFRASubdivision E. Section71-4 "YTeyaper::D2i0a00r-y20R0e1pirond-upcthiaosne, 2000-2001 analytical phase, Final Reportin 2003 SmEpxepcielersoi:menMnatlsallaroddfe(sniLga5ns6:p2l,Aaoyrrlh1y7n6mcpahlolmsa)rfdosrwaepreerioedxopfossexd wteoek.PRCOSoinn tcheudaritceoannolnlogmyrion,aulp ojaoraidiefmoreomsdsaonencmranoeposny.rtesgTtrheoeudpcfsoeneaedn.oldAatfnhde1.1i8 7xa.6nwepdemk6s.2orfepeaxppmmoesntuetraetg,mreobunlptossodveewrraesemcuaobilalnnetciatizenededdfnoodmn eTheihrm,eisnbpgoediymvawleleaeirxgdshpowasenerderfcoudheCaonunigoneudlmpittahinodnmesnwudbeojerfecWteeevsdaelktuoat1ae.dgwAoesetskhlyn.becroBepescyga.usEieofefhcmWetesetikoon2no0avdghuelret nrstesiwneereTkhreemfofirtnhaednasltayutdsyi6.swveTeahket,seetdhceoanvldladanalatlaiycsooilsloeefctxtahemediondneitdeaadvactahasrrediosvmmiedtenhdtefgcirooonutwpoloduasrneidnagr1e7tt.he6e poFdsmiitemmaaalmilaeordnss.wceorlCelonbesrdoeuqaguheWtntenltkyo22t0h8(eiTayraieepnrgo1)dw.uacstivienipihaaesdeapprpiroorx0imatteelnysoeneofwteheek satftuedrytbhye Furye.edaEuhgeeWaxepdoeskdurien.goTfWgethcpekrsot|ducadtnyi,do6wneowraefsheccavelaseltcatwteeedrdeoancndosweleteflkolaeyndibcanancsutasblafeytoizrodend.hefdourRrPeapFtrOioSodn.ucotEifvhsee Cernatdrpmiomitnaatsts,eiilnnyctluh1d2inegwteogekc.opnroodrNueccttiToohpne,siseeemsborfwyfesorperviipnaegriflwoyer,rmeehda.mtacohinambiionlfyfe,pdrhiaonlngln,fraoonmmdcsaaucrdvhivmaflae,teedwrenfraoel deine.tidopseogrofuap.se,SaamnpdleBsuwresreofakFaabnroifcserf,o rhisno,paktihdnoegy,iaglonaedx,ampinratoiovn efnom,bgotahl
\
000344
Wceireshanndaoftfeindg,foLciovnecrewneiirgthosnswoefrPEreOcSo.rded for ll necropsied mallards, and the fers FPAraohteuenedreFooflcluWotwehieankngsa6c,dolblalenocdtoidnonesoaompfpslbeeldsoo.wdAeStrptchellt,ictbeidrodfrsnoeimncrtoahplesys1,u8rvveiavrnisdngv6ae2druelptcpomblilretdcst,eeawdhmnesdn OhFeWcoenotkrrol2a0an,nedmAit17cn.6eacnpapypmssy.t,revaAetGmrientstaomgnpraoluebpslsowpoerdirosraamtlposloeeucstohwlaleneracestiesadsafonocdmonledclariolepsbfyiodalmstahfdoeurlbctehgbieinmrnidicsnagil.a SmormleyeetaschgPwrroeiuptatfonreuagtnraotnuspi,s.aAodAdliltfthibolnooafolsdlpyrc,iolnslge.ectbseldooffdoosmmama1pd0luelostfnwperdrienogcpowlileenrctgecdowlfalosecmtsee1d0afroofefmdsparncitnhog Coun and emacyteplaee factions
TaTabbTllere1e1.EaExxtppeersiimmeenntEaslxpddeoesssiigengffoorrtheedidieettaarryycchhrroonniicppiilloottrreepprroodduuccttiivveesstuuddyywwiitthmmaalllalrsds
2)
T-e
Peervieeod Tindisooplalyhowliog,yEndoopfolinbctosodEymxpaomtrigonannes,d, Gg0e TprodOuctiEon, embryo ability, hatchabily, offpring. heath and
6B2r
sGweosk asulrvtivaaln,dPoRfOfsSprcingomnaclel,nitPRriOvSaecrtoannicdeonbtrnoatsdionsseinr5uo5mf Components (membrane, lumen, nd ol
Test Bird Age: Aditmalas (approximately 27 wecks)
Number ofRepliateFsi:e replicates prs) pr catment grovp
Birds per Feed and
Replicate: One mae and female lls perpen Water: Food and water were provided ad ita
dung
ll
pases
of
he
study
to
eSok lt mands nd oping. Feed Consumpion was messed on perpen basi, once a
eAnearymtiinceald Mboynrietvoerriengp:haTseetHsPuLbsCtaanncdemcaonscsenstpreacttiroonmeitnryfed, lv and serum samples were
FDStaeationstrioceanl tMopirctohcoeaddusbr:eUwspeinosinfuisceadnot0mccpioflmrpcoaefrstthbeeetteewsate,meaennnttarneaafltymsetinst ogwfriotuvhpasrc.ioanntcDoeuln.(neAtTNth'OesVsmAtuu)ldeiwnptal'ses
ehew17.s6 ppumiadmtoematkegrsousps wcercocmopmaprairseodns in those instances where only th contol and
Aoovemraecge tDhaepilnmylIIennaahknaedt(erADneI)eaotfpaPigRrreOdoSuwpiftowhriitcnhaoacuhtpernae.ksitnmFegonoitdigocrooanucspcuomwupanttsiopenosttaeinnmtdaitaaeldduiluftsfimrnaeglnldcaaetrsad
cBoldoyulwteeidghat dfaotlalowwes,re averaged ove the exposure durationofthe study an the ADI was
2
00034 .
ADI (mg/kg/day) = Avere ageCBr oontsyua mwpetiigg ohn ((e b9i)rF diee ) eeyGd one. (ppm)
(Equation)
foerstweDeikleyPrpepraeraptiaonr:oaTfetthseitdtoienesttsiwt.erpreparedbymixing PFOSinto remit wasused
RMESeULTaeSDtistsCoutnceerndtraeytioWdnsi:dTeo cermoattnihoencfdonLciie.nraTthoemnsioitfPofEqOuSannthte atodcinh(eLm0iG)caolr BrBeOean15g% Woepresk.g1hoTfhthhpesetsauudndyrwpeaecsroi0voed.r7s9As0paplmPy.RseODSuorfiinnctgoenWtomleadte6eitshkssephiLokwOeedGdfitoosrpParFneOgsSeeinncfteoohmfe ea eeoSoaitaonrodmoirs9daebtteeorr1msi1on9ec%dhotnfntgehcsonmapoyouunwdnesr.:arSPgLFOdOQoSs,esLc. o5n6Tch0eet0mideoan1n7s.m6eipnasptumereii dedfPeeRodOcSd MeoartnalssoanuedpsCdiniigncagel tObewseesreovyatc.oinosnWishd:eiNrleeod dse3uvelTraomluolmtaoelflscuapotacievdiutryheedsaidesoshre cfaoonontdorllesoiponesPEtnOdeS Seeamenion. ieIntngtoeehnen1im7ne6aaodpnipmn.gThpcrioachemesse.a,lTshoaenesexehihSbinigtnesadcwbehorededymhtoersretmnoerescl,kyraarpdiedsbbpeiockanimsneegf,roiangnddwirhanpeingd ehniesoptnpneeyalaNanoertdandaupwpeeehsaevdAodrmvoetrrhsraeoluFgpfheoructt oCtohntcedetunrttsretaritmoiinnoanotfit(oNhn.OeAAsElulCd)yh.feorrBdamsuaesld lowsnastvh1es7ree6 ArdualteeBoedyoWreiaagshttlsefAett tbcoeotnhtWTrooemlcakhns6yeaonfwd a2h0e,a tiecsmaewnnetrgternorouedpturscwetaihmoeennnticn.orfemelpmiaaelrdeedrbeotdoducycotinWoetniosglhitn e Stoec nier oowesorsekspo4aninnvbet,odt1ye3wremeigtgehPtl1RovOsaeSssantmettomcneongntfi.edmeaHrlodewsefoivnebt,ehhw1eis7em6deunmc-tgeihoniPewFdaO.sSnKIonEt rear a vtnoaysgtaahviseneesdikginieffitcfcanst r0ewdeeuaecitnmieoenntam.taisHlioceawlbeloyvdsyeigdwrnuei,ifigchatnts.amtaWCleoemspakma6pr.leeBdy(iz0Wecseeo(k5n1)s0, LFiEgin5a.PnbHoJOdsSwhamenatlhmotftemaflswteahesmca1ol3n%ats.esin AthteaWn1e7te.e6kmm2ge0,iPntFhemOamSleKabntordmeyaaltWemeeibngtohdwtyenweetisegahtctsotncatlGolely TtroupoBnaessedLCooOnnNdouEdcty)afwoferoightlthe.seldwulcats2i0o1n7w,1e6etmhkgesoiPftFtaOrhSyKsgLyo.w,SeisnttceeObodsnieeltryavtrahybelNe1O7AAdEvmeCgrsfPeorEEmOfafSleecgts oFrnolskaeo ve doeind. The diciny NOAEC for adult female mallards vas 176 mg
3
000346
Table 2. Daily exposure, feed consumption body and Iver weigh for adult mallards
Soxpomyto PFOSA (iun gthke)dietr . * hindT ) TSex a NA ata M
WeBeokd6yWeWg e@) T= DOML9T
Trew
23:47
18
a00962
TEs)
MFoOI0II23467E5T 1005223 32513225155368
Foilesol
982883
62 [o2ss: IEse M Fo E1038E295
2312924429405
CCr1e7Tt6e,TAlv30.e65vs0er%e1%fo2ea3n4nae8n4d sTsnWaMeFddwoeh1nv0e8o7i8ne5a1nTetTwe18i0p9g4h722bea1ch3e.994sw22e30e46r22i8209e25d
ee yke(bTmohFOSWeokyowre15i6ghtfoyer s3s4$210p,4mc.kgroc2upsnvassbpvasioned voscnd:
pohaery
eNFAeNekdpcopne sucmpt,ionde doatmiaeisnntornasanktm Wseeekn(62.d 00h9afohs emLcOGo.ns.
FhtaemdendftCeeo.dnssuHomnopswtueimvopent:rio,nOmnatlhWaltearewdkasssin1s4i,tgnh1ie6f,ic11a77n.,t6lpy18p,rmeasndecr1a9t,mhmaeanlltdahiradtdsnofointmhtahaelvl1er7s6a cpionpmmtehsetprocenoadtnmitenonlgt
(rccraemaetntignrbooudpy,wBeiagshetd,wonitchhsesurgegaelsst,stthhaediehetairnycrNeaOsAeEwCaswtaesdeetselrmoifnfeededowbaest1a7g.e6ipnotmh.is
LeSiatvcersreaWeoeniignahdlutl:tvWihvweeeringwhectiogmihpntaifrneemdaanflyoestrhefrscooemnnttrthoelggrr1oouupp.n,dtWhh6ir.le2ewpetphremre1t0wreaaasptmpeasnrtteasnt,tisrttihceeasleslydesiifngfneitrfeienccaednst
wemearrlmeleimtno,setbeaietntlghyeinednfuudellotrfopWrtehoeedkutci6im,vinweghcioocnfhditwthaieosnt,eprewmroinuialotddiboonefohefixtgphehcectsegdg0rporuohpdasu.cvteiogBnro.attheTrhgerfrotefwoesrseew,revhreees
`mtahleaTsvewra.sB1a7s.e6dppomn.thes esl, te ieary NOAEC for Iver weight in mal and female
G6marwlolhsaisredPsmaawtehlaralesosguyb:ijneAtcdthiecdto0nmtagrlolrlaoarsndsdneicn1z7t.oh6eppyp1..m8Atnrldeaf6it.nm2deinpntgpgsmrtworeuerpaestwmceenrotesnswaemsrpeliteosdbadceartieifWnicercieeddkeesn2ttd0aWl.encAlkdl
oCotnsriedlearteed tion,ceialemneanlto.reIantcmiednentce of small estes was ned during gros necropsy, but was
TSauecbnhjcmeiaeadlttaeo nogrfosgwrrionunepgcrahopappdyr.obxuimRmbealstueeltlfsyooft1r2omwTentehkdesinoeolcdnr)o,ppse3yr ssriehnsogtlwmeeedmnattlalgtarrodsuepvferwroealmreatshlaml1p7dl.6edpfraponmmd {ectment had broken feshers, had an unkempt appearance and retained its 3olk sc. For
4
000347
mle, onoewmleenfftihpeeisnEgnrdionmgshwee1r7e.6cpopmmetresmneindtetlaalckaenddthneploseropoartmioenno.f helf Hristeoopantrholnogdyp:moseV,alraienodus(uutlrisnssueeoasf,Fmienancltbu)dirmnagiltlwhaceerdrisfseauarnm,dsplbe,eixdanm,fionKemiddnadeuuynl,dteg(rocnaoandi,eg)hptraonmvidecn1rt7oiscpcaolppiesm., TFESohmoaetmrvertwfeeiermeoailnnneosclsieeasrndiod.nsadoiuTnflhttfehriemenalliwgveeerr,oakfnaidldscnoehfyneo,ornpareosvmeemxnea.tlrlniacrurdHlsuotsw,oerdgvaelorllf,efbsslipatordnhidsenegrn,iaonthvceaiardnaye,ndycibeporoafsioenft,5hoeradsPBiuFproOsosSafe lTSpeomitonoaalse,nsdmecimrnetauseeldd sempavleremsatowagfseanrreeessisrianincndhtoirhngd1e7a.c6esappspemednssee(smcmioenmnipftle.ertoeIusaabbdsvnilcee,dofeasmmatetue.rse
doenoepdoaf osmfmatlh cl ntomsraose lmotpeheaysn1i7ot6lhoperpaemlduapcheedenaommaeestnosemnrcoocefomntpshaniersteestdnettmoiwnciitofhteprrolousss.rteWuphrbioldseuwcttehiervseee Eeieortendyin.sth to1hf.7tahpvepemrd]ttrenadttoimneengtsv5saocgoghmepetarShegdtni0ftctaeheniccloonfetgshr.eessesHfioiownnedvimenrga,ycofudadveenovbeebeen conadruyctLedOAduEeCtwoatshedsmtalelmsiamnp1leebdseiz1e7.(6Np=5p).m PBFaOsSed on the effects observed in `males, the RFineEptrBhoedmu6cc3etinvpcepamtReosenualuttemsse:tneTtdh,erethieswesretedauync.otiWrohenisltwemaehsnte-nroewlatcseodnasfildeiecgrhtetsdreotdnruecaehtgimgoennptnroerdeuglcieipdo,rnodaisisaginogyn iCoomnamteann8lntdhlx17w.er6rokomfmtheeexcPeFecOdoSendttercoernattsrmooelntlv.vieTa.hle,Wehhwetecnrheacbofimelwpeyar,reh1da4t-tcdohamiyanlogldahresdasulii,vnoatrnhsed Faicneiocnnsdewuoeftrceheeiesnredeheescto1sf5ifaoniwedi6egl2gspfpbomii.nntrseetpattmfeosnritnsicenwthcheeunnbcuoammitbpneahirrsoeodenf t1oa4c-mdoaeynn0v.o.sYaeTv,hienhsege rievtevetrschhheen.ifpcrosontmrntohlthse1a71m67e6pnpp,ommhtereeeaa.tmmewenetrne.wehmartiewnesorirmeinleaodruttcsotiithoasntotfinathseseivgcnoenirtfrioclrasno.tr.maWTlhhceeesnde TPio nipgnisnsgehgensecdsauyd.edset,F3inranelud,umcbtteierorne nowftehreehaRtmocuhamipbpneagrrsehnoetfntdheeayat,cmhelniantngrd/3e-wankdumebmcebirreyosso,fonnutsmhuebrvebirovidonygf neprtaducSifvhearcinegotrhsefdvie,anrlyeNmOalAlErCdswwahsednetceormmpinaerdedtooeco1n7t6oplpvmalues Based n hese oHTvhaeetrcehiwne1gr3eawnmdeo.Jlsuvp1epn,rileemnMlalcldaamredmnatRenesyuoaltfset:h ePecFtOsSornestehntbs.dyWwheeinghct oofmhaptcthaolicnrgosenasnd,d hoeer weemneintTmeenwtigrehltatfedeelec1.t8sponpjutveenialemlivger weriwgahstoasatauinsyicpclolynegcretaiteornafnsehdat
s
000348
ofcones othenfecat wawsatnoat dbosie-deipeondvenitsandfmoamyhihsavgerobuepe.n ThfeundcetiaornyofNtOhAeErCetfoer
chanid vnelmallardsvas 17.6 pm
able. Mean iver nd body weightsofhatchlingandjune lls fom varius PFOS
treatment groups. :
"Treatment.
Body Weight (2)
Juvenile Liver
e 6T2s HRBatiichktienegs e 11J0u06v3e5n6i4l79e12 PBWeeiag si5a00
E E1E 76 er3sme0esae1essnspphaoE neelcmL1e0lo 30a3m5pa.05 peer
Concentrations of PFOS n Blood snd Liver:
aredumttsasl0abSstsowcrcnaumedlxptolcsdurPesRaOnSdvatisniasceodmocpsoaen-rdceeedppeftnidvoennsst,mnahdnensesrium(Taatbceloden4a)e.vecrTtaogieoadnmaicloynlleaeckttheeed voeoteaBTpRteOSswsawerearamceaonldrloiueat aecWtkeeer2k0m.i6naTwihhoislne.aFfonotryttshhcwoa1ns8traaonlsdae6d.2op1n7phme.pspummtrpmateiinmoten,nshtea,rtuhbmey
erttaseemceaunncaeitonamayyeeernbceaesnssvbeshepn. aavirrlontdmenwtco,oumlTmhboeenlsreypspuslrteosdsoc1fit.negsraaedgrryeseSsaitooefn. ee fo PHOS concentra in both sr and It and ADIof PFOS are given bla:
Male: Serum PFOS(ug/ml) = 41.62 (ADI, mg/kgday) + 0.4595 Liver PFOS (ug/g) = 2.717 (ADI, mg/kg-day) - 0.4814
R=07177 R=0.6676
hustherewas dente concuonbetwennrasingdoseand serum an ver ves. Female: Serum PFOS (ug/ml) = 42.80 (ADI, mg/kg-day)- 12.47 Liver PFOS (ng/g) = 3.012 (ADI, mgkg-day)-- 1.124
R3=0.7827 R= 0.6048
` 000349
`TTarbela4et.meMnetan concentrations oLfivPeFrOCSonicn.l"iver and serum frSoemraudmulCtomnacl.lard(ssgim)
(ppm) Sex Control M
ge) 01570292
Week <10Q
Week 20 T9264
1.80
F M
012020165 08360738
17<2L405Q41
105165
620
F M
023720105 11920422
3654371 37:118
F 065020654 176 M 9.594596
1772250 1014662
158700
Como 3
76pp
F rete
9.684821 Groupswere sscred
of
3094205 Week20 while183d63pp
1444678 resenwterse
2ariL ffocrsersd0 utmWewcakQ s06,0A1llyvimaslelgiveen means and standard deviationsawetweightbass
mSex-especaiafitcsstduifudfyetreerrnmceiesnawtiedorne. obWsietrhviendthfoersabomtehtrlievaetrmaenndtgrsoeurupms,PPFFOOSSccoonncceenntrtartaitonisoinanss cliovnecresontframtaiolnesswinermeale1.1steoru4m twiemresegraepaptreorxitmhaatnelytho1s.e1inttohSe tlimeis ovgfrefeaetmerarlestsh.an=PPFFOOSS concentrations observedintheserum offemalessampledfrom thesametreatment groups.
A closer evaluation reveal sex and time
doefptehnedernetlatdiioffnesrheinpcebse.twTehenesPeFdOifSfeerexnpcoessurmeakaendittidsisfufeicuclotncteontdreavteiloonps
tpirsesduiectciovneceqnutarnattiitoantsi.veInreplaatri,otnhsihsipvsarbiaebtiwlieteyniscodnuceenttortahteioenxspeirnimeennvtiarlodnemseingtnalwhmeartreimcaelslaarndds
inantdhe6.c2 ontprpomlatnrde1at7m.e6ntppgrmoturpesatwmeernetswexeproeseedxpfoosredonfloyr260wweeekesk.swhVairlieabmialliltyaridnsitnhethPeF1O.S8
tconhcerntorautigtohnhedsoattuuadytw.asFoalrsoindsuteantcoet,emapcoormapladriifsfoenroenfceWs einekPF6OaSnadccWuemculkat2i0osnebryumbodtahtasefxoers
control manner
and but
17.6 that
ppm there
wtreeraetmdeinftfsersehncoews
tihnatthPeFpOaSttewmasofaaccccuummuullaatteidonin
a time between
dependent male and
f1e.6m-afloeldmailnlcarredass.e inInsemraulme PmaFlOlSarcdosncfernotmratthieon1s7.o6veprptmistrteiamtemepnetr,iotdh;erheowweavsera,pptrhoixdiimfafteerelnycea.
was `ppm
ntorteastamteisntticeallxy hsigniiafinbcaapnitp.rtoxOivemeardteth5e-fsoalmdeintcirmeeaspeeriinods,erfuemmaPleFOmSalcloanrcdesntfrraotimontsh.e
17.6 This
Satistcally presence of
significant additional
pinrcorceeassseesinthsaetrduimffePrFenOtiSalcloyncreegnutlraatteiotnheindifsepmoaslietisonios findPiFcOatSiveinomfatlhee
Sanedrufmemtoaloethmearllianrfdosr.matAiocnocmoplalreicsteodnodufrtihneg ttehmpsotruadly tsruegngdessotfs tPhaFtOeSggc-olnacyeinntgraitnifoenmsailnesblmoaoyd
bdeoasengirmopuoprtoacncturpredrdurioinngtchtehdeeisfiprosstsi1ts0iowneeokfsPoFfOtSh.e Fstouridny,stdaencclei,npeedadkureigngg tphreodnuecxtti6onweinektshi,s
taondchtaotnaglelysceiansseedrouvmerPtOheSlacsotn4cewntraetioonef.thkeAsttuWsdey.ekC6h, adunrgineigsnpeegagkprcogdgucptrioodnucwteiorne, rseelratuemd
PFOS `males.
cAotncWeenterkat2i0o,nswhien nfeemgaglpersodwuecrteioanpphraodxciemaasteedl,yco3n.c3e-nttirmaetsiolnesssofthPaFnOSthosien
observed in blood serum
offemalemallwaerreodnlsy 1.1 timeslessthanthosemeasuinrmaeleds.Thesedataindicate
7
000386
hcoantcaenstrcatgigonpsroodlucetivotnheadtewlcreersaeseedqiuinvfaelmeantltoesm,ltehevraeluewsa. s anincreaseinserum PFOS
Asa resultofthseexesspesiefc differences in PFOS accumulationinmalar, it is important ttooxitcaokleogiicnatlo sciognnsiifdiecarnacteioonf btlhoeodresperorduumctoirvelivceornPdiFtOioSncoonfcefnetmraatlieosnswthheatnmianyterbperectoilnlgecttehde 252part of environmentalmonitoringprograms.
APnFaOlySsiCsonocfent1r2a-twieoenks ionlJduvjeunvietneisle mallard serum and liver indicated that PFOS
concentrations persist in juveniles post-hatch (Table 5). However, the serum and liver
oPrFigOiSnalcloyncdeenptorsaittieodnsinitno tjhuevecniglge fmraolmlaarndsexrpeopsreesdenftemoanlleymaallfarradc.tionPotoefnttihael tlootsaslesPfFrOoSm
dtheeposjiutvienoinlei.n
tmiaslsluaersdsothienrcltuhdaen
lsiuvcehr
processes or sera.
Taos
asgreoswstht.hediqluuatnitoint,atdiveepurrealtaitoinonsahnipd
rbeegtrweesesnionjuavneanliylseislivwearsancodndsuecrtuemd.PFTOhSiscaonnacleynstirsatiinocnlsudaendd aellggthyeoldkaac,onicnecnlturadtiinognsd,ataa
twheaattmweanstagbroovuep.thTehleimrietlaotfiodnestheicptbioentw(eLeOnDe)ggbuytollkesasntdhjaunvetnhieleLOseQr,umfraonmdelaicvehr PPFFOOSS
concentrations are
`LSievreurmPPFFOOSS((uugigmi=)/00=9103).10(2E4g7g(yEoglyko,lkp,gkmg) /0m-0)15072928
RREE ==0098080074
iTnhtuhse eligvgeryaonlkd.serum concentrations in 12-vieek odmallardsare highly correlated with levels
dPeFpOeSndecnotncmeantnrnaetri,onwsitihn tthheegrievarteastncdonbcleonotdrasteirounsm oocfcjuurvreinnigleinmamlallalradrsdsinfcrroemastehde i1n7.a6dpopsemmeaxlploasrudrsew.asThe a23.1vaenrdagreanrgaetdo roofmP3F.O3S2 0co3n9c.e1n.trTahtiiosnasveinrasgeersuemrutmo ttohaltvein rlaivteorwfrasjsuivmeinillaer 1othat observed oradultmallards 27 wiartanhge of 233 0.50.7).
Table 5. Concentrations of PFOS in liver and serum of 12-week old
juven"irleeammalelnatrds. * Tiver Cone
Serum Cone.
(Cponptmr)ol
<(gQle)
(0ug/0ml)
6128
<<LL00QQ
0000570374220000740616
So176nna 00262n 0016s 8 ti 0496+n 01b8e4seon
Od Q or eu rwas0of0p enisgewhia amcplresm).vDost0h0g1ihmenolna wet nigh bis.
C17o.6npcpmetnretatrmoeanfttPs FidOuorSniinsnthMeaflilrsat radnEdgsgisxt:h Ewgegeskowfereegsgampproldeudctfirono.m tEhgegcsonwteorle,s6e.p2araantedd
8
60035/
ito tre components, gg membrane, albumen and yolk, and ch component vas analyzed
for PFOS (Table 6).
"Table 6. PEOS concentrations (ug/ml) in mallard egg components *
`Treatment (ppm)
`EggMembrane: 1 week Gweek
Albumen 1week Gweek
Yok Iweek 6week
`Control <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ
62
0.040 0077 <LOQ <LOQ 8.34
16.1
ve 0o001o16) 0(00.00m198) oowmn oommy aa72s)zo657017)
mmtsonmn a A ST aT
#LOQisthelimit ofquantitat(0i.o0n1 ug/ml)
ehFornregaigrffyseanmecpexlspedobseattewbeocetonhnchWeneeracktoinc1oenansntdraan,tdiaoPnRspooOsfSitPicvFoeOnceSenmtlreasotsironnesdiwinnacsdgiof.besreernFvtuercdtahbgeerctmowomrepee,onnhaeednurtlest
aJnedeta.WcheWehakemn6ePbRnyOtSvccmlonecnewnitttrhaetvyieoolln,ks hPfaoerviccnaogcnhhcegenigaircooanmspsondcneonnetcgewnecreoamicpooonmnpewanhrtiesdcfbaoerltiWwmeeeeennkWh6edewktahs1e
aSemnrelnlywrheeierPFtOhSn ctoonsceenmaeisosnesdslaitghWteycrkedu1c.edTahteWheeeekpownhetno chiosmpwaarsedobtsoeWreveedkf1o.r
For the 62 ppm treament, tere was approximately a. 19-0id mrese in FOS
`JcFooornmcYeonaltkmratethinoenr,sbweattshwraeepepwnraoWsxeipemrkaotxei1lamynatd1el6ofyolr6d.6efgngomedseienmcnabsceroanincnademnyneiomrleubkiro.annsHeoocveowrnescnvmfroetreortnh,sepewr1ih7o.di6 "sThtehimpldiclatifoensmaolfemtahlelsaerodbsheardvedytdinftisenhcisersesdtiefafidcyulsttatewiintherpestpebcut s0seeumm0,ivndeircaotr cPaRgOcSomcpoonnceennttaPtRoOnSincobnoctehnetgrgatmieomnbbryatnhes eanndd oyfoWeweekre| tfiolr isntcererasdionsgeogprouunp.l WTencieksd, hInepaare, hdseittroenrdsaaprpromxoismtatkcelylanewlefpdierfcchgatphroeduacdtuiltonfanldehdhndotoyleyt rbeescchoedn ad site TAeaftuirothnesripevbaleutawtieonn eogfgthyeolckggPFPORSOScodnactaensshtoownesdatnhdatPtFheOrSe ewaxspoaneela3tsivmleyascuornesdtabnty `arsapgperdoailmyantiekye15(fAboDoD)thFtohrew6e.e2ka6n,dt1h7e.v6peprgerayiolmkenPtF.OTShcosncmeintorawtaisonStoiAmDiItlorhaattiro oTatpo odfvredgresfsoiomn amoredgereswsiisonsapnarlyosimsaolftyhe13y.o1lk PFOS concentrations and ADI where the
Yolk (ug/ml=) 13.082 (ADI, mg/kg-day) + 2.259
R? = 08568
cHoonwceevnerruiotnhs vraesstsiognsihpsmobreenwaerenile:xpFosoeinsatnadncee,ghgemgeimmbemrberaannedADalIburmaetn fPrRtOhSe 63 pom teamnt was 0078 hie fo te 176 ppm wesment it was 0095. A ati
)
:
000353
cCoonmcpeanrtriastoinoncs.oudBansoetd boen ctahilselparteeldimifonraryalabnuamlyesnis,duiet ctaonthbee lcaocnkcloufdeqduatnhtaitfiyaobllek PPFFOOSS concentrationswere hebestindicatorofvironmental exposure. mSeinacseurneod rineatmhiesntSt.arceyl,atqeudaandtvieirvsee eeflfaeitosnswheirse bobeseerevnedcfgorSaonnycerenparioodnucstiavnedpearcatmesteirn TotchofiJnuvegnisle ivrasnotgiven nisreport MCiOlNeCaLnUdSfeImOaNleSm:allardwere exposedto PFOS atdiaryconcernsof0, 8, 62and h1e761p5pm3.0dT6h3e cponptmrotleaanmden1t7:s6 wperpe rexptosemd fmoarlnlalryds6wweerekesxpporsieord tfoorte1r9miwnaetiso,n.whNiloe Comnceentnrateilonitees.temd.oraWlhiiecnscoomrpaorveerdtoithgcooifrtl ocaymew,erehegbsweerreednoatPRanOyS. vPaFtOedS eTctcswoenreeeodbsceornvseudmptifon aeetamhtearnaWyedoeslke&overalWuseecdk 2in0tohefstthedss.tudAyt.WNeoekwe2a0,mmeanlte.srenltatheed 17m.6aploimientahteme1n7t6hpapdmreadeuacmeednbtodaydwn nerewaisheedgnicnochmipdeansrceedof0scmoalnlirsle.sInwhaednticoonm,paarleed Stoicgonnrilfs.iocfaWthnhiceliencvreesaisceulairncriedgerenscseioin tihse&17n.o6nmpaml pthryesaitcmlegnitegarloupproovceers, tahteobbisoelrovgeidcailn {Si4gncifoincaen!ceogrfotupiconulddnoiisbdenFclgy etvoalmueartperdedtumfoahenosmGallesasmplrteestmze,eenfnaotdtof,mhee reNnOteAdEeCffeacntdswLeOrAECgbsfoerveaddulotnarnedprjoudvuencile iennd(phoiisntss yev.alaBtaesdedinohethseusdeyraer lgivh,een (`ATdavbelrese7E).ffecTtheLevNeol (ObLsOerAvEabLlevaAlduevserfsoer PEFfOfcStmeLeavseulre(dNiOnAvEarLi)ousantdissLuoewmeastticOebssaerevaabllseo
reported (Table 8).
0 000353
c"Tharboln7e.pMieossstuuedmyewnitthenmdapoliantrsnanddhaersopoidincetaray NtOAEeCadndLOAEC valfourPeFOsS in
Dietary NOAEC
AMDorUaLliTty Endpoint
Gem
2176
Bodywight
FeMmiaelses 176
LFeievderwcoenisguhmtpion Gross pathology
ES17i6 Sins
Hisopatlogy
FeVmiadleess61276
Reproductive
Se
OFFdSaPyRsuIrvNiGvability Hatchingvenile bodyweight
S21i7r6e
SivcenleooenrveipoTTratSeoi, We TO En Br Toe
Oe stn ty spe eon dy wh
Dietary LOAEC
pm)
Males 176 Males 176
Ee WoT
n 000354
`pTilaobtlest8u.dNy OwiAtEh LPFaOnSd. LOAEL values in various matrices i adult and offspring mallards in a chronic
Measuresof PFOS Exposure A ADDoUsLeT(pMpAm)L*ES SAeDrIum(m(guPmFlO)S/kg body widay) Liver (mg PFOSKg)
NOAEL 62 039497 Lig
LOAEL 176 3I6s80 96
ADULT FEMALES Dose (ppm)
176
SAeDrIum(m(gu/gk/gmlb)ody wt. day)
316304
Liver (ig/e)
97
OFFSPRING Yolk (ug/ml)
507
SLievreurm((gafgei)n)
00.042%62
Ccoonncternotlrnatdi1on7s.poorgteFdOnSa vertewleiyght=bbaasss, on Weck20,whieh62pp els sed on Week 64s. AT
Nio eandvLaolvweseffect values frdict ndADI are bse onconceneation. Seradvr ffect values we measured
LOAECformales based n 3 satstialysignifcotection inbodywight
DATA QUALITY: Reliability: Klimish ranking = | REFERENCES: `GwailtlhagthheerM,alS.lPa.r,dV.aWniHlodviefne,TRn.tLe.m,aatniodnBle,avLedr.s,P1rBo.je(c2t0N03o).. 4P5F4O-1S0:5A; 3piMloRerpeoprrtodLucatbivReeqsuteudsyt No. U2723. Gallagher, SP. 2001). Extractionofpotassium Perfluorooctancsulfonate from mallard serum `aAnndalymtailcallarLdabolriavteorrifeosr, Iannca.lSystiusdyuNsoi.ng023H-P0L4C2-Elecirospray/Mass Spectrometry. Centre Gmaelmlbargahnere,, aSlPb.ume(n20,1)a.nd yoElxktrafcotrioannaloyfsisPoutsaisnsgiuHmPLPCe-rEfllueocrtooocstparnaesyuMlafosnsatsepecftrroommetrcyg.g. Centre Analytical Laboratories, Inc. Study No. 023-063, OTHER: Last changed: 05/05/04
2
.
000358
