Document reQgZQLgDVz9R67G1obmBJLVV

PFOS: Determinationofthe n-Octanol/WaterPartition Coefficienbty theShake FlaskMethod - A Non-GLP FeasibilitSytudy inSupport ofWildlifeInternationaLlt,d. ProjectNumber: 454C-108 OCTANOL/WATER PARTITION COEFFICIENT TEST SUBSTANCE Identity:Perfluorooctanesulfonatmea;y alsobe referredtoas PFOS or FC-95. (1-Octanesulfoniaccid,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-p,otassium saltC,AS # 2795-39-3) Remarks: The testsubstance isa whitepowder. Sample was taken from 3M lotnumber 217. Sample was storedunder ambient conditions priortotesting...Puddteyterminedto be 90.49% by LC/MS, 'H-HMR, 19FNMR and elementalanalyses techniques. METHOD Method: OECD 107 GLP (YIN): See Remarks Year completed: Study completed 1999. Report completed 2000 Remarks: A feasibilitteystwas conducted to determine ifthe physical propertieosfthetestsubstance were compatiblewithshake flask methodology proposed foruse inan n-octanol/wateprartitiocnoefficient determination. Upon completionofthetestprocedure,a definitivpeartitioinnterfacweas notobtained.Instead,a beige/whitemulsion was observed throughout the sample. RESULTS Log P,,: Not determined. Remarks: The observationofan inseparableemulsion inthe preliminary testprecludedconduct ofa definititvest,as indicatedinthe protocol(No 454/120298/107F/SUB454, 3M Lab Request U2723). Therefore,a study cancellatiorneportwas generated by the laboratoryconductingthe testing afterconsultatiownith3M. CONCLUSIONS The studysubstance exhibitsphysical/chemicaclharacteristitchsatmake determinationofthe n-octanol/wateprartitiocnoefficienitnfeasiblbey the Shake FlaskMethod. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:JimischrankingNA. Study notfeasible. REFERENCES Study conducted atthe requestof3M Company by Wildliflenetrnational, Ltd.of Easton,Maryland. OTHER Last changed: 5/3/00 WILDLIFE INTERNATIONAL, LTD. TestSubstance:PFOS ProjectNumber:454C-108 PFOS: Determinationofthe n-Octanol/WaterPartitionCoefficienbty theShake Flask Method A Non-GLP FeasibilitSytudy inSupport ofWildlifeInternationaLlt,d.ProjectNumber: 454C-108 Introduction A feasibilitteystwas conductedwithPerfluorooctanSeu.lfonicAcid PotassimnSalt(PFOS) todeterminief thephysicalpropertieosf thematerialwere compatiblewith .2 shakeflaskmethodology' proposed foruse inan n- octanol/waterpartitiocnoefficiendtetermination.The testwas conducted by WildlifeInternationalL,td.for3M IncorporateadttheWildlifeInternationaLlt,d.analyticaclhemistryfaciliitnyEaston,Maryland. The testwas conductedon March 01,1999, Raw datageneratedby WildlifeInternationaLlt,d.and acopy oftheletterreport arefiledunderProjectNumber 454C- 108 inarchiveslocatedon theWildlifIenternationaLlt,cLsite. Materialsand Methods A 100 mg/L stocksolutioonf PFOS (WildlifIenternationLatld,No. 4675) was preparedby dissolving 50.0mg ofthetestsubstancein500 niL ofNANOpure water.An analyticbaallance(ID:R-LAB-05) and a ClassA glassvolumetrifclaskwere usedforthepreparationT.he solutiownas sonicate(dID:SPUB-06) for approximately20 minutestoensuredissolutionT.he resultinsgtockconcentratiownas notcorrectedforpurity. A I0-mL aliquotofthestocksolutionwas transferreidntoa40-mL glasscentriffitguebe.A I0-mL aliquot ofn-octano(lSigma-AldrichL,ot# 05949MN) was transferreidntothe40-mL centrifugteube.The samplewas hand shakenandthencentrifuge(dIEC Model K, S/N 07570) forapproximatel5yminutesatapproximately1500 rpm. A defuiitivpeartitioinnterfacweas notobtained.Insteada,beige/whitemulsionwas observedthroughout thesample. Resultsand Discussion The observatioonfan inseparablemulsioninthepreliminartyestprecludedconductofadefuiititveesta,s indicateidn theprotocol(No 454/120298/107F/SUB454,3M LabRequestU2723). Thereforea, study cancellatiorneportwas generatedafterconsultatiowniththeSponsor. References 1. Organisation for Economic Cooperation and Development. 198 1. Guideline for Testing of Chemicals, 107: Partition Coefficient (N-Octanollwater): Shake F7askMethod 2@ - U.S.Environmental ProtectionAgency. 1996.ProductPropertieTsestGuidelinesO,PPTS 830.7550, PartitionCoefficien(tn-OctanoLlWater)S,hake FlaskMethod. ReportApRLctval- STQI DVECTOR DATE