Document re0X8EMEKeMVjnmoRVz6g9NzV
AR - 0757
TO XICITY TO AQUATIC PLANTS
TEST SUBSTANCE_______________________________________________
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203CE. (1Octanesulfonic acid) (CAS # 2795-39-3).
Rem arks: The 3M production lot number was 519. The test sample is FC203CE. Current information indicates it is a mixture of 1.84% PFOS, 30% diethylene glycol butyl ether, 60.23% water, 2.56% Sultone foamer, 3.1% sodium octyl sulfate, 2.1% polyoxyethylene monooctylphenyl ether, 0.12% sodium lauryl sulfate, and 0.05% tolyltriazole.
The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect die toxicity of the fluorochemical component o f the test sample.
METHOD:________________________________________________________
Method: OECD 201 and ISO/DIS 8692 Type: Acute Static GLP: No Year study performed: 1989 Species: Selenastrum capricomutum Source: Originally obtained from ATCC, Rockville, MD, maintained in culture medium at 3M Environmental Laboratory, St. Paul, MN. Elem ent basis: Algal cell counts (cells/ml). Exposure period: 96 hours S tatistical Methods: Not given. Analytical m onitoring: pH and temperature.
Test Conditions:
A lgal N utrient Medium: Nutrient medium per OECD 201 and ISO /DIS 8692. This nutrient medium provided all mineral nutrients essential for algal growth and also served as the diluent for all algal operations. The pH of this synthetic algal medium was 7.6 adjusted to 8.3. One hundred mL of this pHadjusted nutrient concentrate solution was added to 900 mL of Millipore MilliQTM water. Final pH 8.3.
Stock and test solution preparation: A 1000 mg/L stock solution was prepared by diluting 1000 mg of test substance in 900 mL of Millipore MilliQTM water then adding 100 mL of the pH-adjusted nutrient concentrate solution. Final pH of this stock solution was 7.9. Aliquots were then added directly to dilution water in test vessels to create test solutions.
001097
Exposure vessels: 250 mL Erlenmeyer flasks containing 100 mL test solution and capped with stainless steel closures.
A gitation: Shaken continuously at ~ 100 rpm. Num ber o f replicates: 3 for the blank controls and 2 for test material treatments Initial cell loading: 1.0 x 104 cells/mL. Num ber o f concentrations: five plus a blank control Lighting: 400 foot-candles from continuous cool-white fluorescent lighting. W ater Chem istry:
pH range (0-96 hours): 8.3 - 9.4 (control exposure) 6.8 - 7.9 (1000 mg/L exposure)
T est tem perature (0-96 hours): 24 C (incubator)
R E S U L T S _______________________________________________________
Nom inal concentrations: Bk control, 100,180, 320, 560, and 1000 mg/L
A lgal Growth Response a t 96 Hours
Nom inal C o n e ., m g/L Bk. Control
100 180 320 560 1000
Avg. Growth Rate
1.3853 1.3398 1.2703 1.0891 1.0129 0.8045
% Change in Growth R a te
--
3.3 8.3 21 27 42
Element values are based on nominal concentrations.
R eversibility o f G row th Inhibition: Not given.
Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
CONCLUSIONS______________________________________________ _ _ _
The test substance exhibits a 96-hour percent change in growth rate compared to the control of -4 2 % at the 1000 mg/L treatment.
Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
001098
DATA QUALITY Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, the study lacks information on purity of the test substance and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES____________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number G1358-1,1989. OTHER___________________________________________ !______________ Last changed: 6/26/00
001099
ENVIRONMENTAL LABORATORY
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STANDARD NUTRIENT MEDIUM FOR FRESHWATER ALGAE
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NUTRIENT
CONCENTRATION
FINAL CONCENTRATION
IN STOCK SOLUTION IN TEST SOLUTION
STOCK SOLUTION 1 MACRONUTRIENTS
NH.C1 MgCl,.6H,0 CaC l.2H ;0 MgSOf.7HO k h 2p o 4
STOCK SOLUTION 2t Fe-EDTA
1.5 g/L 1.2 g/L 1.8 g/L
1.5 g/L 0.16 g/L
15 mg/L 12 mg/L 18 mg/L
15 rag/L 1.6 mg/L
m
3oo
FeCl,.6H,0 Na2EDTA.2H20
80 mg/L 100 mg/L
STOCK SOLUTION 3: TRACE ELEMENTS
HMr3tBC0l3,.4H,0
ZnCl2 * C o d i .6H-0
CuCi;.2HO Na2M3o4 .2H 20
185 mg/L
415 mg/L 3 mg/L
1.5 mg/L 0.01 mg/L 7 mg/L
80 ug/L 100 ug/L
185 ug/L 415 ug/L
3 ug/L 1.5 ug/L 0.01 ug/L 7 ug/L
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STOCK SOLUTION 4s NaHCO.
NaHCO,
50 g/L
50 mg/L
Sterilize stock solutions 1-4 by membrane filtration (mean pore diameter 0.2 urn).
SOLUTION 5: NUTRIENT CONCENTRATE (Prepare freshly before each test.)
Add 100 mL of STOCK SOLUTION 1* 10 mL of STOCK SOLUTION 2; 10 mL of STOCK SOLUTION 3;
and 10 mL'or*STOCK SOLUTION 4. ..
-
Make up
water. tiSS
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SOLUTION 6* STANDARD ALGAL NUTRIENT MEDIUM Zbi (Oestf O-1
REFERENCES*
{1) ;; O E ^ l?8 4 lfQW row th^
20^iuG idelines|
fo r/ T e stih g o fC h e m ica lsi^ O rg a n iza tio n fo r Economie
Cooprt ionandiDeeloprant/t Paris/l France
(2) ' ISO 1987. Wat er Qual i A l g a l ^ Growth- In h ib it ion sTea
. ISO/DIS 8692.? International/O rganization for ;StandardizatJ Geneve/; Switzerlandl
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ENVIRONMENTAL LABORATORY STANDARD NUTRIENT MEDIUM FOR FRESHWATER ALGAE
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NUTRIENT
CONCENTRATION
FINAL CONCENTRATION
IN STOCK SOLUTION
IN TEST SOLUTION
STOCK SOLUTION I; MACRONUTRIENTS
NH .C l MgCI-.6H,0 CaCl2nfo
MgSOf.7HO kh2po4
1.5 g/L 1.2 g/L 1.8 g/L
1.5 g/L 0.16 g/L
STOCK SOLUTION 2: Fe-EDTA
FeCl,.6H-0 Na2EDTA.H20
80 mg/L 100 mg/L
STOCK SOLUTION 3: TRACE ELEMENTS
15 mg/L 12 mg/L 18 mg/L 15 mg/L
1.6 mg/L
80 ug/L 100 ug/L
CmD CD
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H BO MnClf.4H-0 2nd 2 CoClf.6H-0 CuCl,.2H,0 Na2M004 .2H 20
STOCK SOLUTION 4: NaHCO.
NaHCO,
185 mg/L 415 mg/L
3 mg/L 1.5 mg/L 0.01 mg/L 7 mg/L : .
50 g/L
185 ug/L 415 ug/L
3 ug/L 1.5 ug/L 0.01 ug/L 7 ug/L
50 mg/L
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Sterilize stock solutions 1-4 by membrane filtration (mean pore diameter 0.2 um).
SOLUTION 5; NUTRIENT CONCENTRATE (Prepare freshly before each test.)
Add 100 mL of STOCK SOLUTION lj 10 mL of STOCK SOLUTION2Y 10 mL of STOCK SOLUTION3
and 10 mL of STOCK SOLUTION
Makk<e up to 1,000 mL w 1th -x-M '1I Adjust the pH to 8.3 +
. 'S>V. SOLUTION 6: STANDARD ALGAL'NUTRIENT^MEDIUM
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ENVIRONMENTAL LABORATORY
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STANDARD NUTRIENT MEDIUM FOR FRESHWATER ALGAE
NUTRIENT
CONCENTRATION
PINAL CONCENTRATION
IN STOCK SOLUTION IN TEST SOLUTION
STOCK SOLUTION li MACRONUTRIENTS
NH .Cl MgCl_.6H,0 CaCl5 2H^O MgSOf.7HO k h 2p 4
1.5 g/L 1.2 g/L 1.8 g/L
1.5 g/L 0.16 g/L
STOCK SOLUTION 2: Fe-EDTA
FeClv 6H_0 Na2EDTA.2H20
80 mg/L 100 mg/L
STOCK SOLUTION 3: TRACE ELEMENTS
H BO MnClf.4H_0
ZnClf CoCll.6H-0 CuCl~.2H0 Na 2M004 .2H20
185 mg/L
415 mg/L 3 mg/L
1.5 mg/L 0.01 mg/L 7 mg/L
STOCK SOLUTION 4: NaHCO,
NaHC03
50 g/L
15 mg/L 12 mg/L 18 mg/L 15 mg/L
1.6 mg/L
80 ug/L 100 ug/L
185 ug/L 415 ug/L
3 ug/L 1.5 ug/L 0.01 ug/L 7 ug/L
50 mg/L ...
CmcHOo/D>
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1
s
CTmD
Sterilize stock solutions 1-4 by membrane filtration
(mean pore diameter 0.2 urn).
'
SOLUTION 5: NUTRIENT CONCENTRATE (Prepare freshly before each test.)
Add 100 mL of STOCK SOLUTION 1; 10 mL of STOCK SOLUTION 2? 10 mL Of STOCK SOLUTION 3? '
and 10 mL of STOCK SOLUTION .4.
Make up to 1,000 mL with -Mi Ili
M iu s t the
______ water.
SOLUTION 6: STANDARD ALGAL. NUTRIENTfMEDIUMM F C ^ Q g ^ ^ .
' ? -a10OO KKc. L
Add 100 mL of the pH-adjusted; Nutrient Concentrate Solution
to 900 mL of Milli-A)
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REFERENCES: OECD 1984*
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(2) ISO 1987. Water Q u a lit y A l ^ j ^ ) ^ f i v f | n h ib lto n V T e s t , !>>-; s
ISO/DIS 8692. In te rn a tlo h a llO r'a a ^ iza tio rilfo r Stan d ard izatio n ;
Geneva ,v Switzerland i | i S S M i i i i
001102
BEST COPY AVAILABLE
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ENVIRONMENTAL LABORATORY FRESHWATER ALGAE GROWTH INHIBITION TEST
ALGAL CELL COUNT, No./mL
HBMOCYTCMETER MEIHOD*
TEST DAY.V / )
__ TEST TYPE_ A i d : s ? r
MATERIAL TESTED;
ALGAL SPECIES: Green Algae, Selenastrun capricornutum
fxiyr'yo/ y .DATE^
TR|EUATjM/ELN_T TOTALCOOUFNT25EDFIELDS No./mL
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ALGAL ASSAY: BOTTLE TEST (AA:BT) COMPARISON OF GROOTH RATES
Papa ______ __________________
Data:-J br / v / f s ?
material n stm -.W .Z fl'xiP F , f a / p / f i f t )
ALGAL SPECIES: Freshwater Groen Algae. Sclenastrum capriqomutum
CELL COUNT
GROWIir RATE( * ) -----------
TREATMENT
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BEST COPY AVAILABLE
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.(2)-Eercent..Change,in Growth..Rate..-- '" 1 '
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ENVIROMENTAL LABORATORY FRESHWATER ALGAE GROWTH INHIBITION TEST
ALGAL CELL COUNT, No./mL hbiocytcmeter method'1
test day
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MATERIAL TESTED;/
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SPECIES:Green Algae, Selenastrun capricomutum
LR& / * < & - / DATE .51
TREATMENT
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TOTAL OF 25 FIELDS COUNTED 54
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MATERIAL TESTED:
Environmental Lab - Work Sheet
ALGAL ASSAY: BOTTLE TEST (AA:B1 COMPARISON OF GROWTH RATES
Lab Request No.
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Data:. Analyst:
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TREATMENT
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