Document rXdmQXaYKLnRdG4oQKG2bwer
AR226-2859
Company Sanitized. Does not contain TSCA CB
E. I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine
HASKELL LABORATORY REPORT NO. 622-77
atera I Tested:
atera I Submitted By:
Organic Chemicals Dept.
Jackson Laboratory
Haskell No. 11,300 Sample Ready for Testing: 6-15-77
MUirtbtNIC ACTIVITY OFi IN THE SALMONELLA/MICR0S0ME ASSAY
rFims IA l535eI!!ddTA l O O ^ r e ^ I e d ^ " d e f e c t 'base+na I T h
^ J P ^ u P t w r , were used in the mutagen assay,
' 98
used to detect frame-shllti Z u l U
i Z l l ^S Caalnin aanndi ^Yam^asa1kiTM, Mu' t^at*io*n * Research 3JT^A (1l957357)' TM347-1"3684 ).
>sence of metlbollc^cti J a t ^ , 'o.r m ^ o r r s o l u t l o r o r t ^ t / t ' '^ " 1 h o m o 9ena+e activation system (S-9). In the
ml of top agar (0.6$ agar, 0.6$ NaCI, 0.05 mM L-hlstldine n ns
xd aPProx,ma+elY I08 bacteria were added tc
-face of a Davis minlmil agar plate, "ie aiefebol c i r ! I ,, f ? nM.b, T l,,)- The "n o n was mixed and poured on the
' he chcml-al-top agar solution. The S-9 ml rental f 1 Sys+sTM Evolved the addition of 0.5 ml of S-9 m i x f u m
iver 8 M g C I ^ V m M KCI. i Z , glllcose-6-- ' ,, S e V * f t p ' l 7' 01 " M O O 9 sepernetant of homogenired , is added directly to the top" a9gar ,Immmmeead'aiaTtee >,eeffoorree IItt wVa*s NpAourPed!"odver00the 5miondilmTMal phaogsaprhaptleateI.pH 7 . 4 1 . This mixture
e geneTMi%o,SicTt\t 'ia9nirfoTt. ? l ^ Cr'iyn's.,he COm|,und ,s t e s t M * * toxicity. Routinely. TA 1535 is used to determine
-gative. or solvent control ""and'pos ith L Control s^ a 'n ' 'a 1h oonactlvated system these controls consisted of a
-say to measure any activity of the compound In & . i s e ^ o f ^ e l"? a c H ^ l o i ' m U t u r e ! TM ' ' '* " "'',UIted
'n' SC,iVi,<,C
A H plates were Incubated at 37C for 48 hours.
Company Sanitized. Does not contain TSCA CBI
2- -
Results: Tables I amd II
Summary: ^ m ^ H ^ P t r a s tested In S a lm o n e lla tupbim uviian strains TA 1535 TA 1337 ta irxo
no . T . ,.
tratlons up to 10.000 pg per petrl plate. The S pound" as not liagenfc'llVi!^ l i j o b " 1 1 = e ? ? L i ,i ihl' "
quency? * " Ver
SVStem'
" *'- " * ">*" ='9n?fleant
JFR:DFK:ms Date: Aunnst IQ, 1977
Report by:
Approved by:
David F. Krahn Chief, Molecular Biology Section
TABLE l
MUTAGENIC ACTIVITY OF STRAINS TA 1535. TA 1537, TA 1536.
IN SALMONELLA TYPHIMUFIUM
J A 96 AND TA IQQ WITH '`ETABOLIC ACT IVAT|ON
H20 11,308 -S-9
2AA
Compound Added
pg/plate
10,000
fl
2000
If
400o
IV
6000
ft
8000
11
10,000
11
5 ft
10 11
100 1
TAn 11 t;-z c
20
7 20 16 11 12 11
369
h 2o
11,308 2AA
#
**
c Distilled water (solvent control)
= 2-AmInoanthracene (positive control) = Test plate without S-9 and activators - Average of two plates
HIstidine+ TA 1537
Revertants TA 1538
Per
Plate** TA 98
20 17 24 .
TA 100 163
10 17 13 14 20 16
235
6 20 16 19 18 19
1870
16 29 32 28 24 28
2038
63 153 IIP 106 102 95 2489
TABLE li
STRAINS TA ------------
-----
TA
SALM0^L L A TYPHIMURIUM
557> TA l538> TA 98> AND TA 100 WITHOUT METABOLIC ACTIVATION
h 2o 11,308
MNNG 9AAc 2NF
Compound Added
2000 4000 6000 8000 10,000
2 50 25
pg/plate
If If
II II
fl If
II
ir
TiAn 1
15
17 15 10 10 5 3086
Histidine* IA 1537
Revertants TA 1530
Per
Plate* TA~9l
22 10 22
19 15 7 12 13 .
1384
12 12 11 8 5
1651
16 22 22 13 15
235C
h 2o
11,308 MNNG 9AAc 2NF
#
- Distilled water tso Ivent contro 1)
N Methyl N -nitro-N-nltrosoguanidine (positive control) = 9-Aminoacrldlne (positive control) = 2-NItrofluorene (positive control) = Average of two plates
TA 00
1-0
1-7 1Cl 10" fcS 67 3079
'Company Sanitized. Does not contai*
