Document rQwZdd95bkBdDpDbgjJrqpBe
AR 226 _ OLRE
ACUTE TOXICITY TO FISH (FATHEAD MINNOW)
IESTSUBSTAN 000C 00 E Identity: rMeifxetrurreedctoontaasinPiFnOgSp,erFfCl-u9o5r,ooocrtaansesauclfoomnpaotenewnhticohf FmCa-y30a0l3so. b(e1-
Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: is FC-3003,
The 3M identified
pbryotdhuectliaobnorloattonruymabseRr1w3a3s1.noCtugrirveennt.
iTnhfoermtaetsitosnample
indicates itis a mixture of 1% PFOS, 15% diethylene glycol butyl ether,
d7e9c.y3l9s%ulwfaattee,r,0.20%6%Suslotdonieumfolaamureyrl,s1ul.f5at%e,soadnidu0m.o0c5ty%l bseunlzfaotter,ia1zo%les.odium
The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately
reflect toxicity of the fluorochemical componentof the test sample.
METHOD:
000000
Method: OECD Guideline 203.
Type: Acute static
YGeLaPr: cNomopleted: 1997 SSuppepcliieesr:: PIinm-ehpohusaelecsulptruormeselmaasintained at AScl-DETD, Duluth, MN oAnxaylgyetni.cal monitoring: Temperature, pH, conductivity, and dissolved Exposure period: 96-hours
`Statistical methods: The LCs; values were calculated by Trimmed
`ESxpaecatrtmeastn-uKsairnbgeTromxsettahtods.tatiTshtiecaNl OsoEfCtwvarael,ueVserwseiroen c3a.l5.culated by Fisher's
TLeesntgtfhisahnadgew:eiJguhvte:niles Average length = 16.6 mm
Loading: 0.12 g/L
Average weight = 0.036 g (wet)
Pretreatment: None
Test coDnidliuttiioonnsw:ater: Dechlorinated City of Duluth, MN tap water, post-
carbon filter. The water was aerated for 24 hours prior to the test. +
Dilutionwater chemistry:
AHlakradlinneistsy:: 4484 mmggl/LL aass CCaaCCOO;
_
pCHon:ductivity: 81.1098pmhos/cm
Lighting: Cool-white fluorescent lights. A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period.
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Stock and test solution preparation: The test material was added
to the dilution water on a weight/volume basis.
Concentrations dosing rate: Once.
EStxapboilsiutryeofvetshseetless:t c4hLemgilcasasl jsaorlsuctoinotnasi:ninNgot3 nLotteedst. solutions.
Numofrbepliecatres: 2
Numberoffish per replicate: 10.
WNautmebrecrhoefmicsotnrcyendturraitnigontsh:e sfitvuedypl:us a blank control
pH range (0-96 hours):
7.68 - 8.08 (control exposure)
Temper7a.t6u0r-e
7.92 (1000 mg/L exposure) range (0-96 hours): 20.5-20.7
C
Conductivity range (0-96 hours):
119 133
~ -
121 136
pmhos/cm umhos/cm
(control exposure) (1000 mg/L exposure)
Dissolved oxygen range (0-96 hours):
6.98.7 mglL (control exposure)
5.18.6 mg/L (1000 mg/L exposure)
directly
R0 ESWTs 000
Nominal concentrations: Blank control, 130, 216, 360, 600, and 1000 mg/L
Element values: 96-hour LCso = 472 (376 -- 593) mg/L. 96-hour NOEC = 216 mg/L
Element values based on nominal concentrations
`RSeumbasrtaknsc:e RTeesmtairnkgswafiseld.conTdhuectveadluoens trheepomritxetduraeppalsydteostchraitbmeidxitnurteheaTnedsntot the fluorochemical proportion alone.
conclusions
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Tmhg/eLtewsitthsaam9pl5e%9c6o-nhfoiudrenLcCesoinfotrefrvaatlhoefa3d76mitnon5o9w3wmags/Ld.etTehremi9n6e-dhotourbeNo472 Observed Effect Concentration (NOEC) is 216 mglL.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. +
Paul, Minnesota, 55133
p0 ata0 Quau0 ry 0000000000 Reliability: Kiimisch ranking = 2. Testing meets the criteriafor quality
testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution.
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REFERENCES
~~~ 0000
DTihviissitoens,t wDualsutcho,nMduNctaetdthbeyrAeSqculesCtoropfotrhaeti3oMn,CDoumlpuathnyE,nvLiarbonRmeeqntuaelstTensutminbger R1331, 1997.
om0 HER 000000
Last changed: 6/26/00
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-
EAnSvcilrCoonrmpeonraaltiTosni/AnSgelDDiuvliostohn
"ASe Study IDF 5010.68
STUDYTITLE ACUTE TOXICITY OF RI331 TO
FATHEAD MINNOW (Pimephales promelas)
DATASTANDARD
h
OECD GUIDELINE 203
5
STUDYCOMPLETED 2
June 26, 1997
TESTING FACILITY
ASI Corporation/AScl-Duluth Envi4r4o4n4meAnitraplarTkesBtoiunlgevDiavridsion
Duluth, MN 55811
Tel. No. (218) 722-4040
Fax No. (218) 722-2592
STUDYIDENTIFICATION NUMBERS
:
AScl Study IDF 5010-068
3M Company Lab Request R1331
S`Sppoonsoorr:Su3dMy CIoDmFpRanyI
1
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AESnevlirCsoarmepsoarTaeisoinn/DDAiuSvlcoatns
"Ase Study 04 5010068
1.0 GENERAL INFORMATION
Study Title
Sponsor's Representative Sponsor's Lab Request
Testing Facility
OECD Guideline 203. Acute Toxicity of R1331 to Fathead Minnow
(Pimephales promelas).
3M Environmental Technology and Safety
Services, Building 2-3E-09, 935 Bush Avenue,
St. Paul, MN 55133; Tel No. (612) 778-7452.
Susan Beach, 3M Environmental Technology
and Safety Services, Building 2-3E-09, 935
Bush Avenue, St. Paul, MN 55133;
.
Tel No. (612) 778-7452.
HN
R1331.
AScI Corporation/AScl-Duluth Environmental
`Testing Division, 4444 Airpark Boulevard,
Duluth, MN55811; Tel. No. (218) 722-4040."
Data Validation and Report Review
Retention of Raw Data and Final Report
Location of Raw Data and Final Report
Alan Mozol
Report Signature:0) -
/.
`Two years from the date study is completed. _*
AScl Corporation/AScl-Duluth Environmental `Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040.
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EAnSvcilroCnomrepnotr)aTieowni/nAgSDci-vDiussohn 'ASel Study TDA 3010-068
2.0 OBJECTIVES)
To 10
determine observable
tehfefe9c6t-choonucren1tr0a%tiaonnd(SN0O%ECe)ffoecfttchoentceesnttrsuabtsitoannsc(e,LCRy1g3a1n,d
LCs), and for fathead
the 96-hour minnow
(Pimephales promelas) under static test conditions.
G`Tuhiidsesltiundey2w0a3s(cOonEdCuDcte19d93a)c.cording to the AScI study plan retained by the Sponsor and OECD.
Test Substance
| l { `Test Organisms
i
3.0 TEST METHODS
4
|
Properties: stable, and
cRom1p3l3e1t,elaymbsoelrublliequiind,wastpeerc.ific
gravity
ca.
1.0
(water
=
1),
T1e0s0t0cmongc/eLn.traTteisotnss:olu0ti(odnisluptrioenpawraetderbycomntarsosl)a,dd1i3t0i,on21o6f,te3s6t0s,ub6s0t0a,ncaendt,g_,_ dilution water followed by vigorous stirring.
|
Source: Healthy, from AScl-DETD
juvenile fathead minnows in-house culture.
(Pimephales
promelas)
obtained
>
`Holding/acclimation: `The fish used in the tests were obtained from AScI's
ainn-dh2o3u,se 1c9u9l7t.ure.ForThtehef4is8h huosuerds ipnrethceedsitnugdytewsterineithiaatticohnedmobrettalwieteyn wMaasy0%1,7
and the fish length was
were not fed for 16.6 + 2.5 mm,
24 and
hours mean
before test wet weight
initiation. was 36 +
The fish 19 mg.
mean
Loading rate was calculated to be 120 mg of fish per L test solution.
Use in test: Used 10 organisms/replicate and 2 replicates/test concentration.
Source: Dechlorinated City of Duluth, MN, tap water. The most recent
chemical analysis is in Raw Data Package.
a
CChaarCaOct)eraisntdicas:pHAtofe8s.t08t.imeA,ertahteedwaftoerr2h4adh aprhiaorrdtnoesusseofin4t8hemgte/sLt, (as --&-
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Exposure Chambers Incubation
|Observations { Endpoint Calculations
EAnSv6ilroCnomrepnotraaltTieosn/iAnSgclDDiuvliustohn "ASel Study IDI 5010-068
Type: 4-L glass jars.
Use test,
in test: 3.0L kept covered,
of dilution water or test solution/chamber. During except when experimental observations were made.
the
Solutions were not aerated during the exposures.
Duration: 96 hours.
:
Daily photoperiod: 16 h light and 8 h dark, maintained using cool white
fluorescent lamps.
i
Temperature: 21.0 + 1C.
.
Biological: Daily- Test organisms with abnormal behavior and signs of
stress, and dead test organisms.
`
dWaaitlye-r tcehmepmeisrtartyu:re,(1D)OA,tctoensdtuicntiitviiattiyo,n-antodtaplHh.ardAnlelsdseatenrdmianlkaatliionnisty,we(r2e) `made according to APHA methods (1995).
LCyg: Inhibition Concentration (ICp) Method.
"
LCsy's: Trimmed Spearman-Karber Method.
"
NOEC: Fisher's Exact Test (TOXSTAT statistical software, Version 3.5, West Inc., and University of Wyoming).
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EASnevliCroornpToesrtAianSgtGDDiiuvlioustinho.n
"ASel Sty IDF 5010-068
3.0 RESULTS
`Test Substance Toxicity (Pertinent data are in
Table 1)
| 24-h LCs:
48h LCs5i 72h LCsz
96h LCypt
> 1000 mg/L (Not Calculable).
> 1000 mg/L (Not Calculable). > 1000 mg/L (Not Calculable).
173 mg/L (Not Calculable).
|
96h LCsy: 472 mg/L (376-593).
Ranges of Water Chemistry Parameters
96-h NOEC: 216 mg/L.
Total hardness (as CaCO mg/L): 44-48.
i
Alkalinity (as CaCO; mg/L): 42-44.
I
Temperature (C): 20.5-20.7.
DO (mg/L): 5.18.7.
PH: 7.60.8.08.
>
1
pecific conductivi os/cm); 118-136.
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:
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4.0 TEST QA/QC
EAoSvcilro'CAnSomeerlnpaSoluraidToyens/IiDAnFSge5lD0-1iD0vu.il0su6oh8n
Mortality in than 10%.
control
exposure
must
not
be
more
|
0%
mortality.
`The lowest DO concentration at test termination must be at least 60%ofthe air saturation value at test temperature. l
`The lowest DO concentration measured was | 57% of the air saturation value at 21.0C.
`The lowest value was measured at the 72-hour test interval for the 1000 mg/L concentration. At the 96-hour interval the DO was 64% of scaotnucreanttiroant.ioTnhdeidrendoutcaepdpDeaOr taot itnhvealhiidgahtetetshte,: test as significant lethality and acceptable DO's occurred at the 600 mg/L test concentration.
`Test duration must be 96 h.
`The test duration was 96 h.
Reference Substance Toxicity Test (this test is optional).
|
Control toxicity
chart for testing is
sinodRiauwm
cDhaltoariPdaecrkeafgeer.ence
*
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,
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EAnSveirloCnomrepnotraaltTieosni/nAgSeDiDvuliustohn 'ASCl Study IDF 5010-068
5.0 REFERENCES
AWmaetreircaanndPWuabsltiecwHaetaelrt.hAAPssHoAci,atWiaosnhi(nAgPtHoAn),.D.1C99.5. Standard Methods for the Examination of -
`OGrugiadneilziantesiofnorfoTresEtcionngoomficChCeomoipcearlast.ioOnEaCnDd
Development (OECD). Publication Information
1981/1993. OECD Center, Washington,
D.C.
Hamilton, M.A., R.C. Russo, and R.V. Thurston. 1977. Trimmed Spearman-Karber Method for
`7E1s4t-i7m1a9ti;ngCoMrerdeictainonLe1t2ha(l4)C:o4nc1e7nt(r1a9t7i8o)n.s
in
Toxicity
Bioassays. :
Environ.
Sci.
Technol.
11(7):
7
USEPA. 1993.A Linear Interpolation Method Concentration (Cp) Approach. Version 2.0.
for
Sublethal
Toxicity:
The
Inhibition
%:
West Inc., Wyoming,
and University
of Wyoming.
Toxstat Software Package.
Version
3.5.
Cheyenne,
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7
*y 002745
r AstrGapvraoT nSek utes
TABLE 1. ACUTE TOXICITY OF R1331 TO FATHEAD MINNOWS (P. promelas)
| res Eee Concentration (mg/L) [wn n TomeT we gor
[| ocwoy[00 | 00 | o@ | oo |
| ws [co | oe [| ie | van | | ow [00 | 0 | to | sw | || 0| ee [ie | se |
A Somatgr sa pitas
LETHAL AND NO-EFFECT CONCENTRATIONS WITH CORRESPONDING 95% CONFIDENCE INTERVALS
[CC oormiiemo,re
TT
owmmen
Sow
|55% comammimena 7]
Noa
|
mice sence
1 [ vw m |eeT wcew ||
mm
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