Document rBqON7j4YgkdE3bMzx69QmJkq
ARI2G-OHOY
MICROBICS MICROTOX TOXICITY TEST
TEST SUBSTANCE
Identity: Perfluorooctanesulfonylamido(ethyl)acetate; may also be
referred to as PFOSAA, FC-128 or as the major component of
eFtCh-y1l2-7N-,[(FhCe-p1ta2d8e,caFfCl-u1or0o9o,ctFyCl)-s1u0l9fo-nXylo]r-,R1p9o0t4a.ssi(Gulmycsialnte,,
N-
CAS
#
2991-517)
Remarks: The test sample is FC-129 referred to by the test laboratory
as R1904. Current information indicates it is a mixture of 42% test substance, 32% water, 14% 2-butoxyethanol, 4% ethanol, and 8% C4-C;
fluoroalkyl carboxylate compounds. Test material was from 3M production
lot 930014, mfg date 3/97.
The following summary is abbreviated because the test sample is a
D`maitxatumreayonfotthaectceusrtastueblsytarnelcaeteintoax
water/o
icity of
rganic solvent solution.
the test sample with that
of
the test substance.
METHOD:
Method: Microbics Microtox "BASIC" Procedure
Test type: Static acute
GLP:
Year
CNomopleted:
1997
Species: Vibrio fischeri formerly Photobacterium phosphoreum
Analytical monitoring: pH and osmotic condition
Replicates: Two
lSitnaetairstriecgarlesmseitohnopdrso:grEaCm10praonviddeEdC5fo0r Mvailcureostocxalculated by statistical
Test organism source: Microbics Microtox Reagent.
RESULTS
Nominal concentrations: Blank control, 125, 250, 500, 1000 mg/L
Element value:
5-minute ECs=o 354 (316-396) mg/L
15-minute ECso = 290 (261-323) mg/L
30-minute ECs = 274 (248-302) mg/L
All element values based on nominal concentrations.
dReesmcarrikbesd:
iTnetshteintgeswtassubcsotnadnuccet
ed on the
remarks f
imeildx.tuTreheofvatlheuetersetpsorutbesdt
ance as
applies
to that mixture and not the test substance. No attempt was made to
000419
=n
ema
determine the impactof the presence of the organic solvents or what pPoerrtfilounoroofotchteanteoxsiucliftoyncyalnambiedoc(oentthryi)baucteetdatteo.the
CONCLUSIONS
The FC-129 mg/L with a
30-minute ECs 95% Confidence
for Vibrio fischeri was determined Interval of 248-302 mg/L.
to
be
274
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St.
Paul, Minnesota, 55133
DATA QUALITY.
Reliability: Kimisch ranking 2. Testing meets all criteria for quality
testing, but lacks analytical confirmation of test substance concentrations.
There is a lack of characterization of the test sample.
REFERENCES
"This study requestof
was conducted at the 3M Company.
AScl
Corporation
of
Duluth,
Minnesota
at
the
OTHER
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FILE: 97070901.K5 MICROTBOaXsicDATTAestREPORT
sR1t9o0c4k dark yellow in color;consistency of cough syrup. 2000ma/l
Teat Time: 5 minutes
Osmotic Adjustment:NaCl
NUMBER
10/17
conc CR/GAMNA % EFFECT AV % g DIFF.
CCoonnttrrooll 19160..9590,7 8798..4353 00..00 00..88008756 &#
21 3
111160.50537/66071.0392 122.197 53145
112255..00000000 250.0000
00..438984 ## 0.848 #
3228..82 30.5 q 2.3 45.9
54 6
110038.09750/753350.4925 110.817 37.84
520500..00000000 500.0000
01..63435 ## 1.388 #
3597..22 42.6 3.3 57.6 57.5 q 0.3
87 110191.1729/722671.7920 11000000..00000000 22..138823 ## 6780..63 69.4 q 0.5
G#RUs=edCoCfOoRrREcCaTlIcOuNlatFiAoCnTsOR = 0.808. 6
ECs0 384.0,KG CONFIDENCE RANGE:316.3 TO 396.3)
Signatur.
_
CONTINUED TO NEXT PAGE.
EST TDIaNtEe:: _s#r/e3./52
000423
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FILE:97070801.K5 PAGE 2
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ESTIMATING EQUATION: LOG C = 1.2247 x LOG b 95% CONFIDENCE FACTOR: 1.11940 FOR EC50 COEFFICIENT OF DETERMINATION: R2 = 0.87877
+2.5491
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FILE: 97070901.k15 MISKOTBOaXsicDATTAastREFORT
TSRet1so9tc0k4Tidnaer:k
yellow in colorscansistency 15 minutes
of cough Osmotic
syrup. 2000me/1 ausustmentsnant
Numer
tor
CONG. CR/GAMNA 7 EFFECT Av % q DIFF.
CCoonnttrrooll. 1a1.09.35/0 B712..7540 olaa 00..77349755 4&
2I o16l.0s3 s58a.6o5 i1ams.l0o0w0o0 ooulri2 aw a32l.e0 sp q zz
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aris g 5.0 40.50 0.0
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FiaAtCoTunOnR
=
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cn Gia ME CONFIDENDE RANGC.Z61.0 TO 322.5)
Signature Nb yom
CONTINUED TO NEXT PAGE.
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000426
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FILE: 97070901.K30 MICROTBOaXsicDATTAestREPORT
Rst1o9c0k4 dark vellow in coloriconsistency of cough syrup. 2000mg/i
Test Tine: 30 minutes
Osmotic Adiustment:Nacl
NUMBER
10/17
CONC. CR/GAMMA % EFFECT av % q DIFF.
CCoonnttrrooll 19160..9590,7 7667..8410
o0.l0o
00l.e6995419 4
2 3
111106..8053/ 5488..0340 122.19 42.33
112255..00000000. 250.0000
00..549895 ## 1.005 4
732..57 Son;
4 56.
110083..79057 2471104016 110817 29015
320500..00000000 70010000
cf.l84As0e 4# 1.638 8
P45E.7 63.1
87 1i0i9i.l1729// 2105..4328 1100000..00000060 22..7909%3 4# 773a.0
#GRUs= edCofCoOrRRcECaTlIcOuNlatFiAoCnTOsR = 0.6950
050 275.0
Awa
8 mye (941 CONFIDENGS RANGE:248.2 10 301.5)
35.1 0 2.4 47.5 4 5.2 62.1 0 ou 7a ,
Signature
:
CONTIN TO NEXT PAGE...
TEST DATE: ZAR
TIME: _/5R0
>
:
000427
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FiLE9070%1.K50 Page 2
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000428
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Environmental LaAbtotraacthomreynAt } DaLtRe//STuidmye:Number: |< 1904 20
Microtox Test Solution Preparation ams:
AAR Ty
TSEUSBTSTANCE: Riqey
000
(10.esPtrienpaga1r0a0%tsioloutnionofrecSortdofcinkal cSonocleunttriaotniaosno1r00%1a0nd0s% kSooielcutpitoino2n) MDialsuse/nVtoUlsuemd:e or volumeivolumeofTest Substance. "4r0al3imfaojdaocoem|r
Final Concentration of StockSol(u mgIt L oi r%ovin v):
Balance Used: eHler ATAS0 Annlyhonl
[CO SS 0
2. pH Adjustment of Stock Solution or 100% Solution
Initial pH:
PH Adjusted to.
PH Adjustedwith:
*
Rd dig
3Ld --
Ha
3. Osmotic Adjustment of Stock Solution or 100% Solution
Osmotic Adjuster: N_ ACL
CO mg added to __20mLsoluion
Conmens: Sechium Chlncte CryamlsTT Parer
4.
Appearance
of
pH
and
Osmotically
[4 Ppaeln
Adjusted Stock Solution
.
CaEARY or OPAQUE
(Circeone)
Color: _yLrgiIdI0LO
Comments: 21904 devi Flows Coles corsysteorsy F
EB Cotes
+ A 5-3
as
_T _ -_---- -MN- r _-- mm mo_ -_m-_
. eermao
000429Page21022
we EAnvlirnCmoenralpTosriAnaSgetlDiDivuiotsinon
MICROTOX PHENOL REFERENCE TOXICITY CONTROL CHART
Date
i
(@) | Mean (mgt)
i
tion Linit
Limit
i
r=
|
|
LPC ooomm1 [|awon1 [|an1 T [ToT mT [0T |T an T |] wo |
000430
EAnSvcilroCnomrepnoarlaiToens/iAnSgeDti-vDisuitohn
MICROTOX ZINC REFERENCE TOXICITY CONTROL CHART
Lfoonm[|oeoo[T ow|T owT [oor T [om|T ] [rD=ae[m| e(ue gll)e|"| Mreana(mmg/ls)||
seid Deviation
Upper Limit
| Lower Limit
| ov
|
{
|July 1997
1s
4.756
7.1 |
. 000431