Document rBq6p0wEze1k4QyORx9myoVjG

; i e to 2 E i; T-- |: - BI0ASSAY RERORT | svamreezp 10 T--] AR22, 6-28389 flo 333.75 | BE. 1. DUPONT DE NEWOURS &COMPANY | NEWARK, DbvawARE: a| i Line gi i yo TTT i i a Lhe SE eB ~~ i g I Keath tstooir8t%y to rainbow i a {Sans gatidnedt). : i I = | | 5 T| soigonaonsics;Setlinia ei.o | 790 main Steaet :2: i oh JWuanree;ham1,197M3assachusetts Py IE ~~ JED8 METHODS AND MATERIALS This investigation was performed at the aquatic toxicology laboratory if Bionomics, ^nc., Wareham, Massachusetts. The susceptibility of rainbow trout (Salrno '.gaig4neri) to 8230 (a light brown liquid teslied as 100^ active.) was measured in terms of the median tolerance limi t (TI<rn) the nominal concentration of the chemical in wat'er .which causes 50 percent response (death) under the test; -condla^t^^-^uring a specified time interval. The predicptiori of a 'ma^: value, and its 35% ";'""".:''-t^y^ .-: confidence interval, was qasec' on coTiye&si'an of the concentrations tested and the correspondjing" observed ;]a^'ee|)t mortalities -to logs and probits respectivrely,-and thtes^sequent mathematical calculation of a 'linear''r^resa:<^'^r^-l'eg|%^m-Tj.he toxicity of the chemical tested was evaluate^ und^r^static bioassay conditions for a 96-hour period, \:, ; ;, The test procedures for thie static -bioa'sssiyA are in complete accordance with the Fish Bioassay Procedure in the 1970 edition of Standard Methods (APHA) except for those conditions described below, i .Company Sanitized. Does not contain TSCA CBI i The rainbow trout used in these test? were obtained from a commercial fish hatchery in Massachusetts and had a mean weight of 1.3 g and a mear, length of 58 mm. The test population was held in the laboratory hatchery facilities for at least 30 days prior to testing and during this peiicd mortality ';:as <2.%. Static bioassays were conducted in 5-gallon glass vessels held in constant.temperature water baths at 11C (^ 1.0). The vessels were not aerated, and there was a single introduction of the test compound into the test vessels. A solvent control, utilizing the greatest amount of isopropyi alcohol contained in any test vessel, was conducted. Fish were .' acclimated to the test sy-stem containing reconstituted water j as the standard dilusnt for at least 24 hours prior to testing. ! Ten fish were exposed in each test vessel. The reconstituted water used in the test system was prepared by adding the following chemicals to deionized water; i Hardness 38 ,'ng/l mg NaHCO- of salt/liter CaSO^ wMgaStOe^r____K_'"_l _ 48 30 30 2 The pH of the standard diluent was 7.1, and t;.e methyl orange alkalinity was 35 pom as CaCOn. Dissolved oxygen values for the various test vessels ranged from 6.7 initially to 5.3 nig/I at the end of the tests. ft Company Sanitized. Does not contain TSCA CB. i o^<- .lilfcC RE^LT^ The predicted TLcp values (95?S confidence interval) are presented in lable 1. T^ble 2 presents a summary of observed nior-taiity for the bioassay after 24 and 96 hours of exposure. A gela til-r-.-'UE suspension was formed imniediate-y after introduction of the chemical, and various methods of mechanical stirring would not alter the substance. .Company Sanitized. Does not contain TSCA CBS J r'ior.'c-irii i.K, ]nc. 790 yair: Stre'.-t Warehafn, .''."a^sach'^se t t-s .Tune, 1973 PREPARED BY i APPROVED BY; Robert L. Bent ley /-> , r^^ ^' /li^C,.7..-.4 Aquqtic Biologist Kenneth J. Macek, Ph.D. Company Sanitized. Does not contain TSCA CB? 'iaL'le 1 -- A;.:i-,te toxicit.v of 8230 to rainbow trout (^aimo gairdh&rj ) ""hese data are based on results '.'1' bioassays c.-oridL.cted at the aquatic toxicology laboratory of Bionomics, Inc., Wsreham, Massachusetts. Corripound 8230 hour96 TL^ - milligram/liter ^vef" 24 hour(rng/1) >1000 >1000 1000 ^ssay conducted at lie (+ 1.0), mean weight of rainbow trout 1.3 g. .Company Sanitized. Does not contain TSCA CBf .^';ib]e 2 -- 'oncentrations ol' 8230 tested and corresponding observed percent mortality for rainbow trout (sa Irnq gairdr.eri) at '< and '-)6 hours du-ing a static hioassay. C'o'npourid 8Z30 Concentration (nig/I) 1000 750 560 ^90 370 280 210 100 - control control (isopropyi alcohol) hour96 ^ niortollty observed 24 hour 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 <" Company Sanitized. Does not contain TSCA CBt