Document rBp61xZ1rERr3kD4pkvanRY0J

TRADE SECRET AR226-3169 DuPont-5019 Study Title H-24615: Static, Acute, 96-Hour Screening Test with Pimephalespromelas Laboratory Project ID: DuPont-5019 AUTHOR: Robert A . Hoke, Ph.D. Study Completed on: October 10,2000 Perfo rm ing La bo r a t o r y : E.I. du Pont de Nemours and Company Haskell Laboratory for T oxicology and Industrial M edicine Elkton Road, P.O. B ox 50 Newark, Delaware 19714-0050 W ork Request Num ber Study Code Num ber: not rC4 -1 - H-24615: Static, Acute, 96-Hour Screening Test to Pimephales promelas DuPont-5019 1 STUDY INFORMATION . Substance Tested: Synonyms/Codes: Haskell Number: Composition: Known Impurities: Physical Characteristics: Stability: Sponsor: In-Life Initiated/Completed: H -24615 24615 N ot supplied by the sponsor M ilky to tan liquid The test substance appeared to be stable under the conditions o f the study. E.I. du Pont de Nemours and Company W ilmington, Delaware 19898 U .S.A . September 2 5 ,2000/September 29 ,2 0 0 0 Company San&ed. \ /i ss not contain TSCA CBi -2 - H-24615: Static, Acute, 96-Hour Screening Test to Pimephales promelas DuPont-5019 SUMMARY The acute toxicity o f H -24615 to the fathead minnow, Pimephales promelas was determined in an unaerated, 96-hour, static test. The study w as conducted w ith 4 concentrations o f H-24615 and a dilution water control at a mean temperature o f 2 1 .5C. One test chamber was used per test concentration w ith 10 test organisms in each chamber. Based on visual observations, the water control and the 1,10 and 100 m g/L test concentrations were clear and colorless at test start. The 1000 mg/L test concentration was a cloudy, whitish/tan color at test start. A ll water quality parameters were within acceptable lim its during the test. Exposure o f fathead m innows to the water control and nominal H-24615 concentrations o f 1,10,100, and 1000 m g/L resulted in 0 , 0 , 0 , 0 , and 100% mortality, respectively, at the end o f 96 hours. N o mortality or sublethal effects were seen in the water control test organisms. The highest nominal concentration causing no mortality at test end was 100 mg/L. The low est mean, nominal concentration causing 100% mortality at test end was 1000 mg/L. Nom inal H-24615 concentrations were used to calculate the LC50 value using the binomial procedure. The results are summarized as follows: Nom inal concentrations o f H-24615, mg/L 96-hour LC50 (95% confidence interval) for H -24615, based on nominal concentrations, mg/La dilution water control, 1,10,100, and 1000 316 (100 to 1000) H-24615 exhibited low concern for aquatic hazard3 in an unaerated, 96-hour, static, acute test using the fathead m innow, Pimephales promelas. a Sm rclv^ J., Clements, R., Morcock, R., and Rabert, W. (1993). Assessing ecological hazard under TSCA: methods and evaluation o f data. Environmental Toxicology and Risk Assessment, ASTM ST P 1179. (W.G. Landis, J.S. Hughes, and M.A. Lewis, Eds.), pp 22-39. American Society for Testing and Materials, Philadelphia. C o m p aq Sanitized. Does not contain TSCA CB| -3 - H-24615: Static, Acute, 96-Hour Screening Test to Pimephalespromelas DuPont-5019 CERTIFICATION We, the undersigned, declare that this report provides an accurate evaluation o f data obtained from this study. Work Completed by: Daniel J.C. Peterson Associate Scientist ~ Date Issued by Study Director: RobernA Hoke, Ph.D. Senior Research Scientist /o Ocu. c^ogp Date Company Sanitized. Does not contain TSCA O il -4 - H-24615: Static, Acute, 96-Hour Screening Test to Pimephales promelas DuPont-5019 TABLE 1 DISSOLVED OXYGEN AND pH OF TEST AND CONTROL SOLUTION SAMPLES N om inal H -24615 Concentration (m g/L ) Test Initiation (0 hours) D issolved Oxygen (m g/L ) pH Test Completion (96 hours) D issolved Oxygen (m g/L ) pH Water Control 8.7 7.5 6.4 7.3 1 8.7 7.5 5.3 7.3 10 8.7 7.5 5.8 7.3 100 8.7 7.5 5.2 7.3 1000 8.7 7.5 7.4a 7.4a a Measurement taken at 48 hours due to total mortality TABLE2 MORTALITY OF Pimephales promelas, IN AN UNAERATED, STATIC, ACUTE, 96-HOUR TEST WITH H-24615 N om inal H -24615 Concentration (mg/L) Water Control 1 10 100 1000 Number Dead/ Number at Test Initiation 24 Hours 48 Hours 72 Hours 96 Hours 0/10 0/10 0/10 0/10 10/10 0/10 0/10 0/10 , 0/10 10/10 0/10 0/10 0/10 0/10 10/10 0/10 0/10 0/10 0/10 10/10 Company San/ffe@0 snof confai^ *SCA CBf -5-