Document rBk89Mndan7dVwrV6aq91axvq
Toxicological Summary
PFOS
Dietary Chronic Definitive Reproductive Study: Mallard
Test Substance: Perfluorooctancsulfonate (POS)
Structure:
1-Octanesulfonic acid, 1,1,2.2,3,3,44,5,5.6,6,1,7,8.8.8-heptadecafluoro, potassium salt. CAS# 2795-39-3.
Test Remarks:
The test substance known as FC-95.
is a The
white sample
powder (3M Lot was stored under
#217), and is also ambicnt conditions
and purity was determined to be 86.9% by LC/MS, "H-NMR, and
elemental analysis techniques.
METHODS
Method: OECD 206, FIFRA Subdivision E., Section 71-4, and ASTM Standard E1062-
86
Type: Dietary Reproduction
Year: 2001 in-life phase, 2002-2003 analytical phase, Final report in 2003
Species: Mallard (Anasplatyrhynchos)
Experimental Design: Adult of0, 10, 50, or 150 ppm in
mallards were the dict over a
exposed period
to of
PFOS at nominal approximately 21
concentrations weeks. Each
4treeaxttmreantpaiarnsd ocfontmraolllagrrdosupwecroentmaaiinnetdai1n6epdaipresroftrmeaaltlmaerndts,aondneupsacidrpfeorr pbelno.odIcnoaldldeicttiioonn,
dnoutriunsgetdhetotesats.seTshsermeaprloldaurcdtipoani.rs Dthuaet wteoreoveurstedsifgornsbloofodtocxoilclietcytinoontedduraitngthtehe1t5es0t pweprme
treatment treatment
group group
by Week was then
3, the weatment level terminated at Week 5.
was reduced to 20 ppm. The 50 ppm treatment
The 150/20 ppm was terminated at
sWiegenskof7,taolxsicoidtyueortaobonvoerrmtasligbneshoavfitoorx.iciBtoy.dy AwleligahdtusltwmearlelamredassuwreerdeaotbWseerevkesd2d,a4i,ly6,fo8r,
`and at from
study termination. the extra pair of
Feed adult
consumption mallards ot
was measured weekly. five-week intervals. At
Blood the
was time
collected of adult
termination, blood analysis, excluding
sampies mallards
were from
collected the 150/20
from ppm
all surviving mallards for chemical treatment groups. All mallards, except
for those from gross necropsy
the at
50 and 150/20 ppm study termination.
tArteatthmeenttimgerooufpsth,ewneerceroepustyh,antiizsseudesanwderseubcjoelclteecdtetdo
for histopathological examination and analyses.
`Adult Once
mallards were egg production
brought into reproductive was initiated, eggs were
phase by photostimulation at weekly placed into sets for
Week 11. incubation.
Eggs were candied and developmental stage and abnormalities were recorded for cach
1
COLE
seemt.bryRoevpiraobdiluictty,ivheatechnadbpioliinyt,saenvdalhuaattcehldindgurhienalgththaendstsuurdvyivailn.clAudfetde:r hactgcgh_ipnrgo,dumcatliloanr,d
wcheirceksdewteerremifneedd.unPtrrieoartetdo
diet for 14 cuthanasia
days. of the
At last
tghreouepndofofoftfhseprsitnugdy(,LocthiJc),k
body blood
weights samples
were collected from chemical analysis.
10 In
chicks from both addition, tissues
the control were also
and 10 ppm treatment collected from these
groups chicks
for for
histopathological examination all necropsicd mallards, and
and the
chemical analysis. liver samples were
Liver weights ware recorded for collected for chemical analysis.
a`Sdaimppolseestwisesruee,aalnsod tBaukresnaoofftFhaebrliivceiru,sbfroarinh,isktiodpnaetyh,olgoogniacda,l perxoavmeinntaritciuolnusf,rogamlladbullatddaenrd,
offspring mallards.
,
Test Bird Age: Adult mallards were 24 weeksof age at test initiation aNnudmbfeemraloef pReerplpiecna.tesI:nTahdedriteiowna,s41p6aiprasirooffmaadlullatrdmsalwlaerrdes mpaeirnttraeiantemdenpterwittrheaotnmeenmtalase partof a blood monitoring study.
Feed study
and Water: to both adult
Food and water were mallards and offspring.
prFoeveiddecdonsaudmpltiibiotnumwadsurmienagsualrledphoanscees
of the aweek
ona per pen basis
Analytical Sera, and
cMogngitcoormipnogn:enCotnscenwterraetiodnesteorfmiPnFeOdS biyn
feed, red blood cells, reverse-phase HPLC
liver and
tissue mass
Spectrometry. Egg components were separated using a method characterized by Bemardt
`and Cook (1960) and Stifani ef a. (1990).
Statistical Methods: Upon was performed to evaluate
completion of the study, an analysis of significant differences between treatment
variance groups.
(ANOVA) Dunnett's
`multiple controls
comparison procedure (Dunnett, 1955; 1964).
was The
used to sample
ucnoimtpsawreerePFthOeSintdrievaitdmueanlt
groups with the pens within cach
btirreda.tmSetnattigsrtiocuapl,aenxacleypstesfoorfabdoudlty bwoeidgyhtwseiwgehrtes cwohnedruecttehed ssaepmaprlaeteulnyitfowramsaltheeainnddifveimdaualle
bobwhite quails. method following
Percentage data (reproduction data) were examined using Dunnett's arcsine square root transformation. Statistical analysis of the data was
performed using Software package
Avian Reproduction developed by Wildlife
Data System (ARDS) Intemational, Lid.
Software,
a
validated
Average basis and
Daily Intake did not take
(ADI) of PFOS for each treatment group into account potential differences between
was male
estimated on and females
a pen within
etxhepopseun.re dFuereadticoononsfumthpetisotnudaynadndadtuhlet AmDalIlawradsbcoadlycuwlaetiegdhtasdfaotlalwowesre averaged over the
ADI ((mgke.mh bodybody weighigh t day)+609) =AAeverrage"FFAoovooedrdaCgCoeonBnssouudnmpyptlwiieooingnhgg{BEfeEeddvBi)rdE_+2d5a9y)) ay ((popomm prPOS
2
601s
Tweasst uDsieedt fPorrepwaereaktliyonp:reTphareattiesotndoifctsthweefrienalprdeipeat.reHdobmyogmeinxeiintgyPoFfOtSheinttesotasupbrsetmainxcethiant the diet was evaluated throughout the study.
RESULTS:
Measured Concentrations of PFOS treatment, feed samples
PFOS in the were collected
Diet: and
To verify analyzed.
the concentrations in the The means and standard
dPeFvOiaStiwoanss ostfabtlhee atensdt ddiidetnsoatrdeeggirvaedne (dTuarbilneg t1h).e sAtnudayl.yses of the diet also confirmed that
"Table 1. Mean and standard deviation concentrations of PROS in the mallard
diet. Nominal PFOS
Measured PFOS Coefficient Percentofthe
Concentration (ppm)
Concentration of Variation Nominal
ppm)
(%) Concentration
Control (0) 10
<L0Q
10240553
544
102
20 50
2082214
103
5094204
401
104 102
TRT iG150meoce16b1m4e1s5m6 mmv9.6e6 rve10m8 h
L0Q indicates mit ofquantitation (LOG 2.00 pp)
Mortalities and in the control or
Clinical 10 ppm
Observations: No treatment groups.
reament related No clinical signs
mortalities were were observed at
observed 10 ppm,
owbistehrvtahteioenxscewpetrieondeotferfmoiotneldestioonbse atnredatomcecnats-iroenlaatledl.ameTnheess.appHeoawreavnecer,anndonbeehoavfitohresoef
adult mallards of both sexes throughout the test were normal.
Within the first 7 mortalities occurred
weeks of the stuly, in the 50 ppm treatment
three group.
incidental and Within the fist
ten treatment-related S weeks of the study,
efxopuriritnrgeamtamlelnatr-drseleaxtheidbimtoerdtaolvietritessiogcncsourfrteodxicinittyhperi1or50t/o2d0eapthp.m Dtureeattometnhtesegrtoruepa.tmeTnhte-
related Week
effects, all remaining mallards in the 150720 ppm 5, and surviving mallards in the SO ppm treatment
treatment were euthanized were euthanized at Week
at 8.
cSoiogrndsinoaftitoonx,icdietpyreatsstihoens,ethdionseasppienacrlaundceed, rleadcurciemdatiroena,ctcioonnvusltismiuolnis,, wlionwgerdrloiompb, rliogisdsitoyf,
ruffled appearance, lower limb weakness, lethargy, and spasms.
(BaNsOeAdEoCn)clwiansicadletseirgnmsinaenddtmoorbteal1it0y,ppthme wNhoilOebstehrevLaobwleesAtdvOebrsseervaEfbfleectACdovnecresnetrEaftfieocnt Concentration (LOAEC) was determined 50 pp.
3
Goa
Adult effects
Body Weight: Compared on body weight in the 10
tpopmconttrreoaltsm,entthergerowuepr(eTanboleap2p).areHnotwterveeart,mesntta-rrteilnagteatd
Weck 2, mallards
there was in the 50
marked and and 150/20
statistically significant ppm treatment groups.
rBedauscetdioonninthbeosedyefwfeecitgshtonamboondgy.
weight, the LOAEC was determined to be 50 ppm while the NOAEC was determined to
be 10 ppm.
mFaeleldarCdosnsinumtphteio1n0:orTh5e0repwpemretrneoattmreenattsmewnth-ernelactoemdpeafrfeedctstoonthfeeecdonctoronlssum(pTtaibolne
for 2).
wHaowseovbesre,rvsetadrtiinngmailnlaWredes kfsro1m
atnhed
12,50a/2t0repaptmmenttr-eraetlmaetnetd.
reduction in By Week 3,
feed consumption some recovery in
feed consumption consumption being
was noted
observed with a in the 150/20 ppm
statistically significant inrease infeed treatment group. This recovery was likely
due to result,
the reduction of PFOS concentrations in the the LOAEC for feed consumption was
feed 150
(from ppm
150 ppm to while the
20 ppm). NOAEC
Asa was
determined to be 50 ppm.
Liver Weight: When were noted in the 10
compared to controls, no treatment ppm treatment group for either male
related cffects on and female adult
liver birds
weight (Table
2). Based on these results, the NOAEC was determined to be 10 ppm.
Table 2. Feed consumption, body and liver weight, and average daily intake (ADI) for
adult mallards at study termination. *
Treament ADI"
"Average Feed Consumption ~~ Sex Body weight
Liver weight
(pm) Control
(maheday) <LOQ
(3 feedbirdday) 20553
M
11642600
29425386
FoOlSS+134 416541051
10
1480.19
193238
MFool14142s72112085
30604463 42211027
50 6364105 12245 MFoO798116%44714090 NAD
150 2094724 1940473 MFM__8181006*++7856 NA
Vwailtuhesconrterovleannd51s0epapnmatnrdeaStmaenntdsasadmepvlieadtiatonWse_cokdy2a0.ndwhTivleertwheig5h0ts3rnds 1t5e0r72i0]pmpemstrrecmameenntt.s
cWoenrteroSlaamnpdl1e0d patpmWegerkoup7saanndd W5,eereksp7ecatnivdelSy.for S0_Faencdd c1o5n0s2u0mppipomn, r(eFsCpe)ctdiavtealy.was for week 20 for
ALD0IQfoavedireatgaerydaPlRyOSintcaoknece(nmteratPioFns wOasb2o.d0y0wpepimght per dy).
+ ANstAeriNsoktiapnpldicaibalec:siagnnotifsieacmasnptledirfefpeorretnedc.e from the control treatmentat p< 0.01
GrrcoatsmsePnatthreollaotgeyd:efNfeecctrsopisnythreesu1l0tspfpomr atllresautrmveinvtinggrofuempawlehsenincdoicmaptaerdetdhattotthheerecownetrroelsn.o
However, incidence
for males from the 10 ppm treatment, of small testes size (7 of 16 birds) when
there was compared
a to
marked controls
increase (2 of 16
in the birds)
4
Gadi
that was treatment related. All other findings were considered incidental and not related to treatment.
bHuirsstaopoafthFoalbroigcyi:usNwoereeffceocntssiidnertehde tlriveeart,meknitd-nreeyl,atberdaiinn, epirtohveerntardiuclutluosr,oaffdsipproisnegtmisaslulea,rdosr
fforuorm ttrheeat1e0dp(4potfrea7t)mebnitr.ds Ienxhmiablietemdaldleacrrdesawsietdh sspmearlmlatteosgteesn,esoinse cchoanrtarcolte(r1izoefd 2b)yanad
reduction tubules.
Tinhteheotnhuemrbceorrtorrolabasnedncoenoeftmraetautredinbgi/rmdateuxrheibsipteerdmadteogzenoearaitnet/haetrsoepmhiynioffertohues
sinemtihneifneurmobusertuobfulceeltlhaltaywearss cohfargaecrtmeirniazledebpiythaenliaubms.encIen oofthmeartubriredsspweirtmh arnedduacdeedcrteesatsies
size, that
spermatogenesis PFOS may have
was normal accelerated
as compared 10 control birds. post-reproductive regression,
a
These results suggest normal physiological
process. Based on these effects, the LOAEC was determined to be 10 ppm.
Reproduction: When significant differences
incoanmypaorfetdhe1r0eptrhoeducctointvreolpargarmoeutpe,rsthfeorremawllearredsnion
statistically the 10 ppm
treatment. Based on these effects, the NOAEC was determined to be 10 ppm.
oOfffhsaptrcihlnignGgsroowrt1h4:-dTahyeroeldwoefrfespnroinsgtastuisrtviicvaollrys
siingntihfeic1a0ntpdpimffetrreenactemsenitn
the body weight when compared
to the controls. There were also no treatment-related effects on the mean liver weights of
offspring from curl, weakened
ctohnedi1t0iopn,pmfotorvelaetgmelenstiognrso,upa.nd
sWlhuiglgeischlniensiscawleorbeseorbvsaetrivoends
isnutchhe
a1s0
npepcmk.
treatment group, these isolated nature of the
observations were not effects in both control
acnodnsitdreeraetdmenttregartomuepnst.-relBataesdedduoen
to the these.
result, the NOAEC in offspring was determined to be 10 ppm.
Concentrations of chemical analysis of
PFOS blood
in and
Blood and Liver of liver samples showed
Adult Mallards: that adult mallards
Results in the
of the 10 ppm
treatment group termination, liver
aacncdumsuclrautmedPFPOFSOScondceunrtirnagtiothnes
isntumday.les
fFurrotmhetrhmeor1e0,
paptm
tthreeatsmteundty
swearmeeatprperaotxmiemnatteglryoutpim(Teasblgere3a)t.er tWhhainlethethmeeraeswuraesdacosnecxe-nstpreactiifoicnsdiinfffeermeanlcees ifnrotimsstuhee
concentrations, the ratio of PFOS was similar, approximately 1.5
in for
blood both
serum sexes.
to
that in liver (S:L ratio) for both Since concentrations of PFOS
sexes were
measured only in mallards from the control and 10 ppm treatment, a quantitative
relationship between PFOS exposure and liver or serum concentrations was not
developed.
5 Couns
Table 3. Liver and scrum PFOS concentrations in adult mallards at
study termination *
Treatment
Tiver Cone
Serum Conc.
(ppm) Control
Sex
wg)
M <LOQ"
(g/ml) 0192039
10
F
<L0Q
M 609195
0.06% 0.04 8734123
Ory
comralend
0
F ppm samples
108845 were analyzed fom
Is
dy.
166120 Allvalues sie
Br1ep0orQ=tedoLniamiwtoetfwqeuaingthittabtaiosns;fo liver twas 0.02 - 00502 g/g while for serum
twas 004-010 4g
sMteuadsyuriendmiecnattedofthPatFOPSFOcSoncaecncturmautiloantsedininblmoaoldlacrodlsleicntebdotthhraoutgihmoeutantdheserexp-rdoedpuecntdieonnt `manner (Table 4).
Table 4. Serum PFOS concentrations (g/mL) in adult mallards at various time points
during the study. *
Treatment
Week
(ppm) Sex
5
10
is
Control
M F
<<QL0"Q
t<oL0gQ <0<Q L0Q
10
MM Fo
935+265 769155
1252206 85.1392
138 18.0 930+3.10
Weeks
Week 7
0
M 388
643
F 305
633
5CLo0mQe=nLrimoitnofwqeuapnteantieodn: LmOQewaasan0d.0a1 njdgamlr.deat.
cBoyncWenetcrkati1o0n,sseixn-spmeaclifeicmdailflfaerrdesnceweirnesesriugmnifPiFcaOnStlycongcreenattreartitohnanbecthaomsee nootbasbeler.vedThien
females. PFOS from Week 10
concentrations concentrations
in to
serum week
of 15.
femTahlees ddeeccrreeasaesedinbysearpupmrocxoinmcaetnetlryat8io5n%s
cmoailnlcairddesdwweirtehptahretitoinosneitngofPFthOeSegignt-olacygignsg dpuhraisneg itnhethreepsrtoudduyctainvdelyinadcitciavteespthhaastefoefmatlhee
Study. In contrast, concentration by Weck
male serum 10 and did not
concentrations reached statistically differ for the
an apparent saturation remainder of the study.
cCoolnlceecntterdaattioenaschosfamPpFliOnSg twiemree(Taalbsloe 3m)e. asured in red blood cells of blood samples
6 60x
Table 5. Red blood cell PFOS concentrations (g/mL) in adult mallards at various
time points during the study. *
Treatment
(ppm) Sex
5
Week
10
is
Control TMM <T0Q
<10Q
<10Q
F <L0Q
<L0Q
<10Q
10
MFo
2154788 1415490
210885 396%251
58.6443 3804394
Week 5
Week 7 _
30
MF 601s6
274 an
TFLFOQS coLnicmeinttorfaqtuiaonntsitaaetiroenp;oLneOdQ3wamsea0.n0s1amndgw.end devon
.
Overall, the measured in
concentration of PFOS in red blood cells. However,
serum was the relative
greater than concentrations of PFOS volumes of serum and red blood cells
in whole PFOS in
blood blood
were could
not not
given in the analytical be conducted and the
report. As partitioning
a result, a mass balance of of PFOS between sera and
RbeBtCweewnassenroutm eavnadluraetdedb.looFdurctelhlerPmForOeS, ctohnecreentrwaetrieonss.ex-AstpeWceifeikc d5i,fmfearleencseesruombsePrFvOeSd
concentrations were approximately 5fold greater than red blood cell concentrations
while for females this difference was approximately 1.9-fold
"The trend observed
in for
PFOS concentration red scrum concentrations.
blood cells throughout the Sex-specific differences
study in red
was similar blood cell
to that PFOS
concentrations were than those measured
apparent by Week 15 with in female from the same
the concentrations in treatment group. By
males Week
being greater 15, male red
blood cell PFOS concentrations were approximately 15-fold greater than those measured
in females. This trend agreed with serum PFOS concentrations measured in these
mallards.
CaonndcleinvterrastaimopnlsesoffrPoFmOjSuveinnilBelomoadllaarndds LiinvdiecraotfedJutvhaetniPlFeOMSalwlaasrdprse:seAnntaliynsitsheobfirsdesru1m2
wpepemkstrpeoasttm-heanttchg.roTuhpeoPveFrOSthactonmceeantsruarteidonisn
in both liver the control
and serum group, but
increased were less
in the 10 than the
concentrations measured in adult mallards from the same treatments (Table 6). Unlike
adult mallards, no sex specific differences were observed in either liver or serum PFOS
concentrations.
7
COLI
Tmaalbllaerds6.*Liver and serum PFOS concentration in 2 week old juvenile
Treatment
Tiver Conc.
Serum Con.
Co(nptprmo)l SeTMMx
<(Lng0/Qg)
0.0(6u8g/%ml0).057
F
<LOQ
<L0Q
10
M 3174130
44140931
F
361126
4972112
C1on0ce0mraliesosntshaonslmimeiat oafnqduaSnaintdautridondfeovriaitivonrs.(5D0ansggaen/dnsgserw)uemr w(10e0 ng)
TchelomwalteheseLrOuQm a(v1-e2r)a.ge (v3)was calculated sing the LOQ where samples were
`The ratio of PFOS concentrations in serum to that in liver in both male and female juvenile mallards averaged 1.40. This value was similar to that observed in adult mallards.
Concentrations of PFOS in Egg Components: Concentrations of PFOS in egg yolk
were measured in eggs collected from the control and 10 ppm treatments. Eggs were
tserleeacttmeedntfgrroomup.WeeYoklsk
19, 20, (whole)
and 21 and yolks were separated and composited composites were then analyzed for PFOS (Table 7).
by In
addition, to better understand the distribution of PFOS in the egg yolk, yolk composites
from the 10 ppm treatment were also fractionated into 3 components, Very Low Density
Lipoprotein (VLDL), phosvitin, and lipovitellin. PFOS concentrations were then
determined in cach yolk faction (Table 7).
`Table 7. Egg yolk and yolk component PFOS concentrations (g/g) in mallard eggs
collected during Weeks 19-21 of the study.
Treatment
PFOS Concentration
(ppm)
Yolk Fraction
g/m)
Control
Yolk-Whole
<LOQ
10
Yolk-Whole
5284058
Yolk- VLDL,
422050
Yolk-Lipoprotein
8870057
`Yolk-Phosvitin
3.590014
"Rowpurelcissiovn eofnthaespmreeapanrsatnidon saannddainradlydseivsioaftdiuopnlsiocnastesweftorwtehieghstambaeviu.niTqhuee sSaamnpdlaer,dadncevgiactoimonpoesxpirteesses
B Thelimitofquantaton (LOQ)was 10 pg/ml
Results indicated that at 10 ppm, there was a significant accumulation of PFOS into gg
yolks from the adult hens as compared to controls. No PFOS was quantified in control
gineadyuolltksf.emTahleesraattiowoefekthe21cowncaesnt3r.a1t.ionTohfe
PFOS ratio
in the egg egg yolk t
yolk to adult
that in female
blood liver
serum PFOS.
concentrations at weck 21 was approximately 4.8. The greatest concentration of PFOS in
8 Coats
the yolk was associated with the VLDL fraction (approximately 77%) followed by lipovitellin and phosvitin.
(CONCLUSIONS:
1M0a.l2l1arwdesekwse.re eDxupeosteodstiognPsFoOfSoavterdtiettoaxriycictoyn,ceandturltatmioanlslaorfd0s,in10t,h5e05a0ndan1d5015p0p/m20foprpump
treatments were terminated at the end of7 and 5 weeks, respectively. When compared to
ocobnsterrovlse,d
wnohenPFOaSdu-lrtelmaatleldardesffewcetrse ofnedad1u0ltppbmodyin
wteheigfhetedor(Tafbelede
consumption were 8). In adult males,
there was an increase in the incilence of small testes size and decreased spermatogenesis
at 10 ppm (Table 8). Thus, while carly post-reproductive phase regression is a normal
physiological phenomenon in birds, PFOS may have accelerated this process when
compared to untreated birds. Finally, there were no treatment-related reductions in
fertility, hatchability or offspring health or survival in the 10 ppm treatment. Based on
mthaelslearcdfsfeactnsd,
othfefspNroingObwsaersvadbelteerAmdivneerdseto
Ebfefec1t0Copnpcmentwrhaitlieonth(e NLOoAwEesCt)
for female Observable
Adverse Effect Concentration (LOAEC) for male mallards was determined to be 10 ppm
(Table 8). PFOS concentrations associated with the No Observable Adverse Effect Level
(NOAEL) and juvenile tissues
aLroewsesutmmaOrbsiezrevdabinleTabAldeve9.rse
Effect
Level
(LOAEL)
in
both
adult
and
`iTnaablcehr8o.nMiceasstuurdeymweintth emnadlploairndstsaannddthaesisrocoiffastperdindgi.et*ary NOAEC and LOAEC values for PFOS
Endpoint
Dietary NmOAEC
Dietary Lm)OAEC
ADULT
Mortality
10
50
Body weight
10
50
Feed consumption
50
150
Liver weight
10
Gross pathology
Male<s 10
Males = 10
Females = 10
Females = 50
Histopathology
10
Reproductive:
10
OFFSPRING
14-day survivability
10
Hatchling/juvenile body weight
10
TJOuvAeEnCilwealsivbearsewdeainghetrased Terence ofsmall 1c 3nd1d0ecreased spermiogenesis adil les rom he
w1e0tpwpemigthrteabtamsenst: both atribute to carly post reproductive phase regression. All concentrations reported on a
9
GOAN
cTharbolneic9.diNcOtaAryEsLtuadnydwLitOhAPEFLOSv.alues in various matrices in adult and offspring mallards in a
Measures of PFOS Exposure *
NOAEL _LOAEL
ADULT MALES
Dose (ppm)
0
ADI (mg PFOS/kg body weighteday)over 21-weck period
1.49
Serum (jg PFOS/mL) at study termination (21-weeks)
873
Liver (ug PFOS/g) at study termination (21-wecks)
09
ADULT FEMALES
DAoDsIe((mpgpmP)FOS/kg body weighteday) over 21-week period
11409
SSeerruumm ((iigg PPFFOOSS//mmLL)) aatt psrteu-dryetperromdiuncattiivoenp(h2a1s-ew(e5e-kwse)eks)
716696
Liver (ug PFOS/g) at study termination (21-weeks)
108
OFFSPRING
Yolk (ug PFOS/mL)
7
LSievreurm((uuggPPFFOOSS//gm)L)'
43.6399
TCoOnRceEnLirwaatisonbsasreepdoorntecdarolny powsetsrweepirgohdtucbiavseiphase regression nau male from te 10 py treatment AT
N"omeaasnudreLdowiesfsfueecvavlauelsu.e fo dict nd ADI ar based on dietary concentration. Serum and live effect values ae
Offspring iver and serum LOAEL values ar averagesoffemale andmale concentrations.
DATA QUALITY: Reliability: Klimish ranking = |
REFERNCES: Bemardt and Cook. 1960. BBA 44: 86-96.
Decker, ER, Flaherty, JM. 2003. Analysisofperfluorooctane sulfonate in mallard and quail ege yolk. Exyeen Report No. 023-070. Dunnett, C.W. 1955. A multiple comparison procedure for comparing several treatments with a control. JAmer. Stas. Assoc. 50: 1096-1121 4Du8n2n-e4t9t1, C.W. 1964. New tables for multiple comparisons witha control. Biometrics 20: Gsatluldaygwhiert,h mSaPll,arVdsa.n HFionvaelnr,epRorLt., WBiealvdeirfse, IJn.tBe,rnaJtaiboenra,l,M.Li2.0,03P.rojPecFtOSNo:.A45r4e-p1r0o9d.uction
10
Cots
Gallagher, S. 2003. Extraction of Potassium Perfluoroocatnesulfonate from Red Blood Cells and Serum for Analysis using HPLC-Electrospray/Mass Spectrometry. Exygen Research, Exygen Study Number 023-066.
Reagen, WK. 2002. Analysis of Perfluorooctanesulfonate in Mallard and Quail Egg. Yolk. Exygen Research. Exygen Report No. 023-070.
Stevenson, L.A. 2003. Analytical phase report for PFOS: A reproduction study with the mallard. 3M Environmental Laboratory Study No. EO1-1256.
Stifani, S., Nimpt, J, Schneider, W.J. 1990. Vitellogenesis in Xenopus laevis and chicken
~ cognate ligands and oocyte receptors-the lipovitellin-L. J. Biol. Chem. 265: 882-888.
binding
site
for
vitellogenin
is
located
on
Wildlife Intemational, Ltd. 1994. Avian Reproductive Data System (ARDS), Version 2.
OTHER Last changed: 5/05/04
n
GOABHE