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Copies to:
AR226-2870
Mete rial Tested
E. I . du Font de Nemours and Company
Haskel I Laboratory for Toxicology end Industrial '-'edicine
HASKELL LABORATORY REPORT NO. 144-78
Haskell No.
!l,92i
Sample Ready
for Testing
1/6/78
Other Codes
1'te-erial Submitted Chemicals, Dve
-------.._--.-.-_... ___--------PRIMARY SIcrN-tRRITftTTON-ftND SENSITiyTION TESTS OH-gtrmEfi-P-t-eS-
Procedure: A range finding test was conducted with the test material to de+erm'ne a no -o minir-.ally ir^ tion. After this concentration was determined, the test for primary irritation was concuc+ed by applyi rubbing in, I drop (--0.05 m"l) of a 100)6 and \0% suspension (vol./vol.) of the test tnsteri3l in ece+one shoulder skin of 10 male albino guinea pigs. To test for the sensi+ization potential, a series of four
injections was given, one each week over a three-week period, which consisted o- O.I ml of a \% solu+ic material in dimethyl phthalate. Following a two-week rest period, the test anirals were challersged for
applying, and lightly rubbing in, I drop C-0.05 ml) of a \OQ% and \0% suspension (vol./vol.) of test m
on the shaved intact shoulder skin. A group of 10 previously unexposed guinea pigs received sir'.ilar ap tiKie of chaltenge to provide a direct comparison of the challenge reactions 'on skin of similar ege..
Results:
Reactions* en Test Areas
Intac-
Guineg Pig
ControSl k/Tinea_s__fo_r__C_h_a_ll_e
:10 Animals - In. Avg. Wt,, = 490 g
(10 -Jew A" n I' re I' s Avesrage '
Final Avg. W+. = 645 g)
^c'.cen-;-^ction in acetone
IQOg
10%
100^
Frfn3ry Irritation Test
24 -3urs 46 r'cur&
C-slle'-.^e -est ^ -"^rs
^- -'...rs
Se-si+ize-ion Response Rc+io**
1+, 9 neg. 10 neg.
2+, 6 neg. 10 neg.
0/10
10 neg. 10 neg.
10 neg. 10 neg.
-, 9 ne=.
K
10 nea.
1C
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2 -
Summary: ^HUBB^'"01^^ slight +o no irri+a":ion In 24 hours and no irritation a+ 48 hours
on the shaved Intact skin of guinea pigs. As a 10'S suspension, no irritation wes observed. A+
animals showed a sensi+iza+ion response. No denna+I+is hazard is expected -from -he handling of
industrial hygiene practices are followed.
when tes
challeng this ma
* Reaction Code: +, ++, +++ = mild, moderate, strong erythema; H-++ = erythema plus edema; -H-H-+ = ne
** Sensi+iza+ion is defined as a significant score increase at challenge over the response expected fro applied initially or on the concurrent controls.
Report by: Approved by:,
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Doris F. Edward Tox i co 1og i s+
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lei I Krivanek Chief, Derma I end Cculer Tox
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