Document rBeYze0m9owJQQ0nEdkqeyOmq

>4e2-|155 ACUTE TOXICITY TO BLUEGILL SUNFISH T E S T SU B S TA N C E_________ Identity: Perfluorooctanesulfonate; may also be referred to as PFO S or FC-95. (1-Octanesulfonic add, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The test substance is a white powder (3M Lot 583) of uncharacterized purity. M ETH O D________________________________ Method: Not provided Type: Static acute GLP: No Year completed: 1978 Species: Lepomis macrochirus Supplier: Not provided Analytical monitoring: pH, DO and temperature Exposure period: 96-hours Statistical methods : Not provided Test fish age: Not provided Length and w eight Average length = 3.0 cm. Average weight = 1.2 g Loading: 1.5 g fis h /L Pretreatm ent None noted Test conditions: Dilution water: Laboratory well water Dilution water chemistry: Dissolved Oxygen: 9.9 mg/L pH: 7.6 Stock and test solution preparation: Not provided Concentrations dosing rate: Once Stability of the test chemical solutions: Not noted Exposure vessels: Glass aquaria containing 20L of test solution. Number of replicates: one Number offish per replicate: 20 Number of concentrations: five plus a negative control W ater chemistry during the study: Dissolved oxygen range (0 - 96 hours): 5.7 - 9.9 mg/L (control exposure) 4 . 8 - 9 . 9 mg/L (100 mg/L exposure) pH range (0 - 96 hours) 7.6 - 7.8 (control exposure) 7 . 6 - 7 .7 (100 mg/L exposure) Test temperature: 19 - 20C 3P* o yj ~ v rn "OO PO -- 2rn -* kO RESULTS Nominal concentrations: Bk control, 5 6 ,7 5 ,1 0 0 ,1 3 5 ,1 8 0 mg/L Element value: 96-hour LC50 = 68 mg/L (95% C.l. 62 - 74 mg/L) All element values based on nominal concentrations Statistical Evaluation of Mortality: Analysis method used to calculate LC50 values and the corresponding confidence limits not noted. Cumulative percent mortality: Nominal Test Cone., mg/L 24 -hours 48-hours 7 2 -hours 96-hours Neg. Control 56 75 100 135 180 0 5 20 50 85 80 0 00 10 20 20 25 55 60 65 90 100 95 100 100 100 100 100 33>* 0 = 3 0 "Qm no "--Omo x* 3 L**rn uO C O N C L U S IO N S The test sample 96-hour LC50 for bluegill sunfish was determined to be 68 mg/L with a 95% confidence interval of 62-74 mg/L. The 96-hour no observed effect concentration was <56 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA Q U A LITY______________________________________________________ Reliability: Klimisch ranking 3. This study lacks sufficient documentation, characterization of the test substance purity, and analytical measurement of test concentrations. R E F E R E N C E S _________________________ This study was conducted by the 3M Company on 5/27/78. O TH E R ___________________________________ Last changed: 4/14/04