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AR 226 - 0916 ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-85, or as a component of FX-3104. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FX-3104, 6%. Current information indicates it is a mixture of 0.48% PFOS, 14.09% diethylene glycol butyl ether, 59% water, 17.6% ethylene glycol, 1.44% sodium octyl sulfate, 4% inhibitor, 2.0% polyoxyethylene monooctylphenyl ether, 1.25% propane sultane foamer, 0.03% sodium lauryl sulfate, and 0.1% benzotriazole. The following summaryapplies to a mixture with incompletely characterized concentrations of impurities. Data may not.accurately reflect the toxicityofthe fluorochemical componentofthe test sample. METHOD: Method: Not given. Type: Acute static-renewalrange-finder GLP: No Year completed: 1985 Species: Pimephales promelas Supplier: Analytical Commerical Supplier, Brady, NE. monitoring: Temperature, pH, and dissolved oxygen. Exposure period: 96-hours `Statistical methods: LCsg values were calculated using logistic regression `methods (probit analysis). Test fish age: Juveniles Length and weight: Average length = 41 mm Average weight = 0.50 g (wet) Loading: 0.67 gL Pretreatment: 25 mg/L Tetracycline HCI Test conditions: Dilution water: Carbon-fitered well water Dilution water chemistry: Not given. Lighting: Not given. Stock and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis. oT Concentrations dosing rate: Solution renewal during the study. Stabilityof the test chemical solutions: Notnoted. Exposure vessels: 4L glass beakers containing 3Ltest solution. Number of replicates: 1 for exposure concentrations, 2 for blank control. `Number of fish per replicate: 4. 002985 WNautmebrecrhoefmicsotnrcyendturraitnigontsh:e sfitvuedyp:lus a blank control pH ran7g.e6 (--07-.986 (hcoounrtsro)l: exposure) 7.6 -7.7 (1000 mg/L exposure) TDeimsspoelrvaetduroexyrgaennger(a0n-g9e6 (h0o-u9r6s)h:our1s9):-20C 7.0 -- 9.3 mg/L 4.2-9.3mglL (control exposure) (1000 mg/L exposure) RESULTS Nominal concentrations: Blank control, 1, 10, 100, 500, and 1000 mg/L. Element values: 96-hour LCso=>1000 mgiL Element values based on nominal concentrations Remarks: Substance RTeesmtairnkgswafiseldc.onTdhuectveadluoens a mixture reported aapspldyestcortihbaetdmiinxttuhreeTaensdt not the fluorochemical proportion alone. CONCLUSIONS The test sample 96-hour LCso for fatheadminnowwas determined to be >1000 moll. Submitter: 3M Company, Environmental Laboratory, PO. Box 33331, St. Paul, Minnesota, 55133 paTAQUAUTY Rneulmiabbeirloitfy:orKgiainmiissmchs eraxnpkoisnegd3a.tTehaechstcuodnycewnatsraatiroann.geT-hfeirndeeriswaitlhaclkimoifted ocfhatrhaectaerizm atiooo nfoffltuu hoerofcnehsetmsitcaamlplper.opoTretsitonsoilnuttihoenssollautcikoann.alytical confirmation REFERENCES TSth.isPasutlu,dyMNw,asLacbonRdeuqcuteesdtbnyutmhbee3rMC1C1o1m1p-a2n,y1,98E5n.vironmental Laboratory, OTHER -- Last changed: 6/26/00 002986 . ==!S= etE2.4S,cuICERRCPLOSRTYON (RAGE FIVER) STL w_COy)-2 AS est Cronin Zag) AT oo sources"Fray oy oteRec'd Alan/g/ patch 285 VgWE.0,ST) gramsAvg.Sizecd,/ cmMo.Organpeirdsosme 4s/ loading Factor an `ishDiseaseTreatment: 2 `estSolution Vo%lLiuterm s/Ale l-Gl, ass Tark "Tank Dimensions: 4.1iter Pyrex glass beaker `lutionWater_Carbon-fwielltlevrateedr (Tew. / C., D.0._Z.5 pm, ph ) mA Pull Signa 24 Hours A - 3 72 96 El Er rE FEE TEETE Ilewd= ~Uilod bz] bars]bil 2lol=ol 0 bud 0 Wed bled bids 2H sohspol0 Wddoll btsalbdsd bids [TT 111 T7171 T1071 1] Test Temp, C |_22 1 207 | 200_ | 20 tials & Date _--1-239/3/R {ro Jars2/7 Je=o-o JT/R TRS RPRENCSEL UU DUR UDEIGIRS.- Lt cade ot 0 trade Lulu mes idlectl Cat aude vga Peedi _ } secondaryEffects (otherthan Mortality): Beso, OPY AVAILAR py TERT Naber TETectad No. 2A-Hr. 48. 72-Hr. 96m. mfp fT zz bagr r 22 INTT A GY Is 12 1S 12 ~1 VvVV 00298' concnw,acitommunion CONTROL. --lo _--sloooo ooo EVIRORENIAL LABORATORY . aJ un1. sunson= sce somone ar os[e0 d10pL--h--lr) www 24m. wm. em, -- sem. o o ' o o o o o o oo o o | egg. (938 CoRIRCE Lat (2) > 1000 Dlooo S000 g (pe were eraSgeeotf tevnrtespliFcisahtivoenrsefwoeresducphacocnocnecnetnrtartaitoinonw.ich ce (10) 48h or 1g ese vere ctnecd see Bopanna (obi fs: & ! >co\000