Document rBKv4zBGJdZj2XrJNj7kL1qXV

AR - 09/7 TO XICITY TO AQUATIC PLANTS TEST SUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FX-3104. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was not noted. The test sample is FX-3104, 6%. Current information indicates it is a mixture of 0.48% PFOS, 14.09% diethylene glycol butyl ether, 59% water, 17.6% ethylene glycol, 1.44% sodium octyl sulfate, 4% inhibitor, 2.0% polyoxyethylene monooctylphenyl ether, 1.25% propane sultone foamer, 0.03% sodium lauryl sulfate, and 0.1% benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. M E T H O D :_______________________________________________________ Method: Not given. Type: Acute static GLP: No Year study perform ed: 1985 Species: Selenastrum capricomutum Source: Not given. Elem ent basis: Algal cell counts (cells/mL). Exposure period: 96 hours Statistical Methods: IC50 values calculated using probit analysis or other regression models. Analytical m onitoring: Not given. Test Conditions: Algal N utrient M edium : Not given. Stock and test solution preparation: Not given. Exposure vessels: Not given. A gitation: Not given. Num ber o f replicates: 2 Initial cell loading: 2.05 x 104 cells/mL Num ber o f concentrations: five plus a blank control Lighting: Not given. W ater Chem istry: Not given. RESULTS____________________________ ;____________________________ Nom inal concentrations: Blank control, 10 0,1 80, 320, 560, and 1000 mg/L 002989 Algal Growth Response Nom inal Cone. (m g/L) Control 100 180 320 560 1000 Mean C ell-C ount (cells/m L) 1,790,000 1,730,000 1,620,000 1,160,000 980,000 210,000 Percent Inhibition -- 3 9 35 45 88 Elem ent values: 96-hour IC50 - 576 mg/L Element values are based on nominal concentrations. R eversibility o f Growth Inhibition: Not given. Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_____________________________________________ _ The test substance exhibits a 96-hour IC50 of 576 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY ___________________ ____________________________ R eliability: Klimisch ranking 3. Study lacks description and records of the method followed. There is no characterization of the test substance purity, and the study lacks confirmation of the amount of fluorochemical proportion in the solution. R E F E R E N C E S ____________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number C 1 1 1 1 -2 ,1985. OTHER__________________________________________________________ _ Last changed: 6/26/00. 002990 *" ENVIRONMENTAL LABORATORY FRESHWATER ALGAE GROWTH IN H IB IT IO N ALGAL CELL COUNT, n o ./ m l HEMOCYTOMETER METHOD* TEST LR_Z/ / / / YTEST nAY * * / - > 7 Y l ___________ TEST TYPF _______________ DATE M ATER IAL T E S T E D :. m c & i o * / - _____ eT to ---------------------------------------------------------------------------------------- ALGAL SPECIES* G reen A lo a e . S e le n a s tru m c a p r ic o r n u tu m TREATMENT COWTTROL. X 3t IC C u ^ i l-l Z--1 (go \~Z, X ~ Z-* 1-3 SU^Ovu^ Uc 1 -4 z-4 XOOO^fM -s TOTAL OF 25 FIELDS COUNTED 160 H6 nu> no 1l4 IW ) US' in 1(jC) 100 Z JL . ZJD no./ml MEAN + SD 1 ,6 0 0 (0 0 0 1 ,1 6 0 (0 0 0 1,7QO,ooo 1 ,1 ^ 7 0 ,0 0 0 li 1 0 0 ,0 0 0 1 ,1 5 0 ,0 0 0 1 ,^ 4 0 ,0 0 0 oi ,00,00 1 ,0 ^ 0 )0 0 0 [,1 5 0 ,0 0 0 i , no, ooo 1 ,1 ^ 0 )0 0 0 % o,ooo. 1 ,0 0 0 ,0 0 0 400, odd 1 X3>\0 0 0 X~CO{ OOO JO, O O O * 0.1 mmdeep Hemocytometer; area of field l x l and 0.1 mmdeep. 002991 ENVIRONMENTAL l a b o r a t o r y op T A B L E 1. SUM/ MjAvR/oY OP ACUTE TOXICITY to freshwater algae (SELENASTRUM CAPRICORNUTUM)*1 * Chemical Concentration (mg/1) (2 ) Percent Inhibition in Standing Crop ' ' 48-Hr. 96- Hr. Based on Baa ed on Based on Cell-Count Cell-Count Cell Dry Wt. Control ISO '.gn -- 35 9*>5 IQftO 8B ICgQ (95% Confidence Limits) m g / 1 ^ ^ Acceptability of Test The Selenastrum capricornutum control cultures have met the following quality criteria: - Exhibited log phase growth within ^ hours (max. 48 hr.) - A t t a i n e d a 96-hr. s t a n d i n g c r o p of S74 *10^ cells/ml (min. 10^ cells/ml) * Lo^ sU ase {vtdcoH v- a4 iH^SjOOO cell^/vkJ!, loadUwQ 20,500 c e ils /wJP (1) Data w e r e a v e r a g e s of two r e p l i c a t i o n s for each test concentration. (2) Based on c e l l - c o u n t (no./ml) a n d / o r ce l l - d r y w e i g h t (mg/1). (3) I C g 0 v a l u e s were c a l c u l a t e d u s i n g Probit Ana l y s i s or o ther regression models of experimental data. 002992