Document rBDax0YbmO9RkdvyedwDzbNzJ

AR22%-01l ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G.,DAPHNIA) TEST SUBSTANCE Identity: Perfluorooctanesulfonate; may also be referred to as PFOS or FC-95 or as part of the mixed product FM-3820 (see Remarks). (1-Octanesulfonic acid, 1,1,2,2,3,3.4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The test sample is FM-3820, a mixture of the test substance in diethylene glycol buty! ether and water (approximately 24-28% test substance in diethylene glycol butyl ether and water). Calculations were made to adjust test values using the upper limit concentration of the test substance (28%) in the test sample and no adjustment was made for the presence of the diethylene glycol butyl ether or water when noted below. These calculations assumed that all toxicity was due to the presence of the Perfluorooctanesulfonate substance. METHOD Method: OECD 202 Test type: Static acute GLP: Yes Year Completed: 1991 Species: Daphnia magna Analytical monitoring: DO, pH, Conductivity, and temperature were `monitored daily. Statistical methods: ECs values calculated, when possible by standard statistical techniques (Stephan, 1983) Test daphnid source: Obtained from cultures maintained by EnviroSystems Division, Resource Analysts, Inc., Hampton, NH. Test daphnid age at study initiation: <24-hours Test conditions Dilution water: Well water from wells at EnviroSystems in Hampton, New Hampshire. Dilution water chemistry: pH: Conductivity: TOC: 78 1200 umhos/cm* <2.0 mglL * Values measured at time of test. Lighting: Cool white fluorescent lights, intensity 23 UE/SIm?, Photoperiod of 16-hours light, 8-hours dark. No transition period noted. 001603 wSatoscpkreapnadretdesitnsdoilluuttiioonnwsatpreerpaatra1t0i0o0n:mg/AL.prTimhaeryprsitmoacrkyssotluotcikon was proportionally concentrations. diluted with dilution water to prepare the five test tEexstpososluutrieonv.esTshelesa:p2p5ro0xmimLatpleasdteipctbheoafketersst containing 200 solution was 6 mL cm. of Number of replicates: Four Number of daphnids per replicate: Five Number of concentrations: Water chemistry during the fsitveudpyl:us a negative control Dissolved oxygen range (0 -- 48 hours): 82-85 mglL (control exposure) pH 8.1-85mg/L (150 range (0 - 48 hours) mglL exposure) 7.8-86 (control exposure) 7.8-86 (150 mg/L exposure) Test temperature range (0 -- 48 hours) 20.8-21.0C (control exposure) 20.7 -20.9C (150 mg/L exposure) Conductivity range (0 -- 48 hours) 1200 - 1300 umhos/cm (control exposure) 1200 - 1300 umhos/em (150 mg/L exposure) Element basis: mortality RESULTS Nominal concentrations: Bk control, 25, 40, 60, 100, 150 mg/L. Element values: 24-hour ECs = >150 mg/L (C.I. not calculable) 48-hour ECso = 49 (43-56) mg/L. Perfluorooctanesulfonate concentration adjusted Element value: 24-hour ECso = >42 mg/L. 48-hour ECso = 14 mg/L. All element values based on nominal concentrations. cBoinotlroogliecxaploosbusreer.vTathieonnsu:mbNeirneotfysfuirvveipveinrgceonrtgsaunrivsivmasl aoncdcutrhreedocicnurtrheence. dofetseurblmeitnheadl veifsfueacltlsyaannddirmemcoobridleidzaitniiotinalolyraonthderafstuebrl2e4thaanldef4f8ecthsouwrse.re 001604 Cumulative percent mortality: NCoomnien,al TmgleL_| st 24-hours sc|o 0 |n5s| PI I I ET Loo [[2 w| Control response: satisfactory CONCLUSIONS bTehe49temstgs.ubwsittahnace9458%-hcoounrfiEdCenscefoirnDtearpvhalnioaf 4m3a-g5n6amgw/aLs. dIfetyeorumaisnesdumtoe all toxicity of the mixture is due adjusted 48-hour ECso value is to the Perfluorooctanesulfonate, 14 mg/L (49 mg/L X 0.28). the Submitter: 3M Company, Paul, Minnesota, 55133 Environmental Laboratory, P.O. Box 33331, St. DATA QUALITY Reliability: Kiimisch measurement of test ranking = substance 2. The study lacks analytical concentrations in the test solutions and sample mixture purity is not and toxicity sufficiently characterized. Additionally, cannot be positively attributed to data is foar cPoenrtfrliubourtoeoctotatnhceestouxlifciotnya.teThase tbhaesidciewtahtyelrenqeuaglliytcyoplabruatmyleteetrhser(choaurlddneaslss,o alkalinity report and calcium/magnesium ratio) were not included in the final REFERENCES TIhnicosrsptoruadtyewd,asHacmopntdounc,teNdHatatEntvhierroeSqyusetsetomsftDhiveis3ioMn,CoRmepsoaunryc.e Analysts, OTHER Last changed: 5/3/00 001605 IL ic es re? 2 I BliR En . pBvBE ES mn, TE aT] Cems om , ge Lamm . --- Lem om wom are pe COPY AVAILABLE Ri ) r00160|6