Document rBD4DGLwv7MyzMGJ0KRwZjnpJ

Monsanto N,FROM (NAME ft LOCATION I Medical, *2SC OATE December 26, 1972 SUBJECT REFERENCE. Toxicity of and Pydraul File CC: J. H. Davidson - B2SF Pydraul 50E \; Two products designated Pydraul 50E and Pydraul 115E are intended for applications as industrial hydraulic fluids and steam turbine lubricants. These products are proposed as replacements for certain of the Aroclor applications and have the distinct advantage of being biodegradable. Questions have been raised concerning the comparative safety of these materials. Pydraul 50E and Pydraul 115E contain organic phosphate esters. Various other representatives of this class of compounds have been found to be highly toxic by both the oral and dermal routes and still others have been shown to be potent neurotoxins. , ^^ - The attached table summarizes the available information relating to the comparative toxicity of Aroclor 1242, Pydraul 50E and Pydraul 115E. The acute toxicological hazard of each of these three products is low. The Pydrauls 50E and 115E. are classed as practically non-toxic by both the oral and dermal routes and are only slight irritants to the skin and eye. There is no vapor hazard from these materials under ambient conditions. Specific studies were conducted to determine if either Pydraul 50E or Pydraul 115E were neurotoxins. Mature hens were given 10 g/kg twice daily for 3 consecutive days, a total dose of 60 g/kg. Following a 21-day observation period the hens were again dosed with 60 g/kg over a 3 day period and again observed for 21 additional days. No gross or microscopic evidence of neurotoxicity was found. Conversely a single dose of 0.5 g/kg triorthocresylphosphate re sulted in reactions indicating, and microscopic evidence of, neural damage. Ninety-day feeding studies in rats with levels of Pydraul 50E aid 115E as high as 5000 ppm failed to cause any gross or mieroscopically detectable evidence of pathologic effects. There were no deleterious effects on food consumption, body weight gain, hematologic parameters or serum clinical chemistry values. Special reference is directed toward the lack of evidence for cholinesterase inhibition in these studies. In summary, neither Pydraul 50E nor Pydraul 115E possess the acute or neurotoxicity properties of certain other phosphate esters. Neither of these materials would appear to present toxicological hazards greater than those of the Aroclors if handled by practices consistent with good industrial hygiene. /bks IN- JO REV- 11-65 Paul L. Wright STLCOPCB4091087 Comparativ' .Toxicity of Aroclor 1242, ydraul 50E 7 and Pydraul 115E Aroclor Pydraul Pydraul 1242___________50E__________ 115E Acute Toxicity Oral LDcq-rats (mg/kg) Dermal Minimal LD-rabbit (mg/kg) Dermal Irritation-rabbit (max. o.O) Eye Irritation-rabbit (max. 110.0) Vapor Inhalation-rat (ambient conditions) 8650 1260-2000 2.3 6.0 non-hazardous >15,800 > 20,000 >7,940 >7,940 1.0 1.3 6.0 10.6 non-hazardous non- hazardoc Fish 96 hour LCqn Trout (ppm) Bluegill (ppm) ^values for 30E. 25.1 31.6 2.5* 3.2* 32 63 NeuroToxicity - Chicken Demyelination Test Dosage Reactions and Mortality Histopathology of brain, spinal cord and ~ sciatic nerve -- 10 g/kg bid. 10gAg for 3 days. b.i.d. repeated after for 3 da 21 days repeated after 21 days -- none none no lesions no lesions Subacute Toxicity - 90-day Rat feeding study Dosage (ppm) 1,10 and 100 200,1000 200,1000 and 5000** and 500C General Response None;pilot Possible Possible study indicated liverwweight liver weight gain increase at weight ir depression and 5000;nonea crease at severe liver at 200 and 5000,none involvement at 1000. at 200 anc 1000 ppm after 1000. 30 days. ** data for NgCP. Cholinesterase (plasma, RBC and brain) no effect no effect OS\N 552671 STLCOPCB4091088