Document rB6N9RD0x9MVDNND8VmMK0VM7

AR R26 _ 0783 ACUTE TOXICITY TO FISH (MUMMICHOG) JESTSUBSTANC0E 00 Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206 or L3243. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: is FC-206, ideTnthiefi3edMbyprtohdeucltaiboonraltotornyuamsbe"rSawmapslenoCt".noCtuerdr.enTthienfteosrtmastaimopnle i7n8d.i9ca1t%eswaittiesr,a 1m.ix3t3u%reSuolft0o.n6e7f%oaPmFeOr,S,1%17s.o5d%iudimetochtyyllenseulgflatyec,ol0b.u0t4yl%ether, sodium lauryl sulfate, 0.05% benzotriazole. 0.5% polyoxyethylene monooctylphenyl - ether, and The following summary applies to a mixture with incompletely characterized concentrationsofimpurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. wemoo: Method: Standard Methods (APHA) 1971 Edition Type: Acute static GLP: No Year completed: 1974 Species: Fundulus heterociitus Supplier: Field collected locally in MA. Analytical monitoring: Temperature, and dissolved oxygen. Exposure Statistical period: 96-hours methods: TL; (median tolerance limit) values calculated using a linear regression equation. Test fish age: Juveniles. Length and weight: AAvveerraaggee wleeniggthht ==315.1mgm(wet) - Loading: Not given. Pretreatment: None Test conditions: Dilution water: Synthetic sea water Dilution water chemistry: oH 80 Salinity 25 ppt Lighting: Not given Stock and test solution preparation: Not given. Concentrations dosing rate: Once Stability of the test Exposure vessels: chemical solutions: Not noted 5 gallon glass vessels containing 15 L of test solution. 001469 Number of replicates: 1 Numberoffish per replicate: 10 Number of concentrations: five plus a blank control Water chemistry during the study: Temperature range (0-96 hours): 201C Dissolved oxygen range (0-96 hours): 4.2 - 8.6 mg/L R0 ESOWTS000000 Nominal mall. concentrations: Blank control, 1000, 1400, 1800, 2400, and 3200 Element values: 96-hour TLso = 1820 (1360 -- 2420) mg/L 96-hour NOEL = 1400 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. conclusions 0000000 `The FC-206 96-hour LCs for mummichog was determined to be 1820 mg/L. with a 95% confidence interval of 1360 to 2420 mg/L. The 96-hour no observed effect level (NOEL) was 1400 mgiL. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 p0 ara0 qua0 t 00000000 Reliability: Kiimisch ranking 2. Testing meets the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the ~ solution. R0 EFER0 ENC0 ES 0000000000 Test was conducted by Bionomics, EG&G, request of the 3M Company, St. Paul, MN, I1n8c7.4,o.f Wareham, MA at the - Last changed: 6/28/00 001470 `a ol = 3 | J De.gen A " i Stauieg ba 9% conbaue enh Cor) sobtand' BIOASSAY REPORT SUBMITTED TO 3 M COMPANY gun pau, urwmesora [Ved i crehau (2) pL | TS 4 wh odTM a-- ] ACUTE TOXICITY OF SAMPLE C TO MUMMICHOG (Fundulus heteroclitus), GRASS SHRIMP (Palaemonetes vulgaris), AND FIDDLER CRAB (Uca | pugilator). J , BEiGon&oGmi,cs Inc. 790 Main Street MWaayr,eha1m9,74Massachusetts 001471 : METHODS AND MATERIALS J These investigations were performed at the aquatic toxicology laboratory of Bionomics, E G & G, Inc., Wareham, Massachusetts. The susceptibility of mummichog (Eundulus heteroclitus), grass shrimp (Palaemonetes vulgaris), and fiddler crab (Uca pugilator) to Sample C, a brown liquid, tested as 100% active, was evaluated i under static bioassay conditions for a 96 hour period. The results were reported as the median tolerance limit (TLgy), the concentration of the sample in water causing 50 percent mortality after 24 and 96 hours. The predicted TL value and its 95% confidence intervals were arrived at by converting the concentrations ) tested and the corresponding observed percent mortalities to logs and probits, respectively. These values were then used to calculate a linear regression equation | Test procedures for the static bioassays are those described for Fish Bioassay Procedures in the 1971 | edition of Standard Methods (APHA). The mummichog, ~ . grass shrimp, and fiddler crabs used in these tests : were collected locally by laboratory personnel. The mummichog had a mean weight and length of 1.1 g and 35 mm,respectively. The grass shrimp had a mean length of 30 mm. The fiddler crabs had a nefFhpace > 0G width of 20 mm. 001472 So Page two 3 The test species were observed in the laboratory hatchery facility for at least 30 days prior to testing. These species were acclimated over a 10 day period.prior to testingt,o laboratory test diluent water quality and temperature. During that period mortality in the test population was<3% and these animals were judged to be in excellent physical condition. The bioassays were conducted in 5 gallon glass vessels containing 15 1 of vater at 20 (* 1.0), and there was a single introduction of the sample into each test vessel. The test containers were mot aerated. ) The test diluent consisted of synthetic sea water with a salinity of 25 /00, a pH of ,0 and a temperature of 20 ( 1.0). Dissolved oxygen levels for the tests ranged from 8.6 at the beginning of the tests to 4.2 | at the end of testing. Ten specimens were introduced into each test vessel 24 hours prior to the start of | RESULTS | The predictedTLsg values and 95% confidence intervals | are presented in Table 1. Table 2 presents a summary J Laroche, G. E. Eisler, and C. R. Tarzwell, 1970. Bioassay , Procedures for 0il and 0il Dispersant Toxicity Evaluations. i J. W. Poll. Cont. Fed. 42 (11): 1982-1989. ; 001473 rt oes 3 Page three of observed mortality for each individual concentration after 24 and 96 hours of exposure. The mortality syndrome in all tests was similar. The mummichog and grass shrimp generally became dark and lethargic, lost equilibrium, and expired. The fiddler crabs became lethargic before expiring. 1 001474 SUBMITTED BY: b) PREPARED BY: APPROVED BY: \i i Bionomics W7Ea9Gr0e&hMGaami,,n SItncr.eet Massachusetts May, 1974 Robert E. Bentley Aquatic Biologist 7 Bevier Hasbrouck Sleight, IIT DFirreeschtwoart,r Programs . Kin - 001475 Table 1 -- Acute toxicity of Sample C to mummichog (Fundulus Y heteroclitus), grass shrimp? (Palaemonetes vulgaris), an fiddler crab (Uca pugilator). These data are based on results of bioassays conducted at the fish toxicology of Bionomics, E G & G, Inc., Wareham, Massachusetts. i Tg - milligran/liter Ho Bifect | Species 24 hour 96 hour (mLge/v1e)l `mummichog >1,800 <2,400 1,820(1,360-2,4200 1,400 | ----m= grass shrimp Contd | Eimuid. fiddler crab 1,000.0 >10,000 280(230-342) 3,260(2,760-3,860) 180 2,400 ) : i i | | { | 2pssays conducted at 20C ( 1.0), mean weight of the mummichog 1.1 Ppssays conducted at 20C ( 1.0), mean length of the grass shrimp 30 mm. Assays conducted at 20C ( 1.0), mean carapace width of the iddl crabs 20 mm. 995%, confidence intervals. - i toed ibe pected oh Ras ofl] an H - J 001476 Table 2 -- Concentrations tested and corresponding observed 3 percent mortalities of mummichog (Fundulus heteroelitus), grass shrimp (Palaemonetes vulgaris), and fiddler crab (Uca pugilator) after 24 and 92 hours of exposure to Sample C. Species _-- mummi chog ) Coe n(cmegn/n 1t)ration 3,200 - 2,400 1,800 : 1,400 1,000 control 2% moTrotaulrityTogobsheermverded 100 100 100 100 0 30 0 0 0 0 [J 0 grass shrimp 1,000 0 100 420 0 100 i 320 0 100 240 = [J] 40 180 140 control 0 20 - 0 0 0 0 J 001477 N Table 2 -- Continued --- Species -_-- fiddler crab (mg/1) Concentration " 10,000 TT %T moi rtalf ity oobsPw erhveer d w 100 100 6,500 [4] 100 4,900 0 100 3,700 - 0 70 2,800 0 30 2,400 0 0 2,100 0 0 control 0 0 eee eee J 001478