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AR 226. 0799 MICROBIAL INHIBITION (DEHYDROGENASE ACTIVITY) TESTSUBSTANCE Identity: ArefmeirxrteudretocaosntPaiFnOiSng, pFeCr-f9l5u,orooroactsaanecsoifmopnoanteen,twohficFhC-m2a0y6Aa.lso(1b-e Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was cc 785-22. The fest sample is FC-206A. Current information indicates it is a mixture of 0.92% PFOS, 11.87.57%5%sodideituhmyloecnteylglsuylcfoalteb,ut1yl.2et%hepro,lo7x5y.e4th4y%lweanteerm,on1o.o8ct3y%lHpyhdenryolxyetfhoearm,er, 0.06% sodium lauryl sulfate, and 0.05% benzotriazole.. The following summary applies to a mixture with incompletely crehfalreacctttehreizteoxdiccoitnycoefntthraetifolnuosorofchimepmuirciatliecso. mDpaotnaemntaoyftnhoet atcecsutrsaatmeplyle. M0 ETHO0 D: 00000000000 MGeLtPh:odN:o TTC. Yearcompleted: 1979 Analytical monitoring: Amountof formazan formed (as determined with a sStpaetcitsrtoicpahlotmoemtehtoedrsm:eaGsruarpihnegd%thteramnesamistutranecde)%. transmittance. TWeasstteowragtaenriTsrmeastomuerncte:PlAacnttiv(aPtieg'dssElyudegPelamnitx),edSt.liqPuaourl,frMoNm.the Metropolitan Test condition: Dilution water: Deionized water Synthetic sewage: Nutrient broth (0.1 /100 mL mixed liquor) added approximately 1-hour prior to start of test. The mixed liquor was kept aerated prior to the test. Sprteopcakraedndbytedsitsssoollvuitngio2n2pgroefptaerasttimoant:erAialprtioma1r0y0 smtLocwkistohludteiioonniwzaesd w1a0t0erm.LTdheeiosntiozcekdswoaltuetri)ontowapsretphaerne dDiilluutteidon(1#1m.LDoiflusttioocnk#s2olwuatsion to Tprheepsaeresdolbutyiaondsdiwnegre1 musLedoftDoiplurteipoanr#e1thteo 100 test mcoLncdeenitornaitzieondsw.ater. Tost vessels: Not given. Number of concentrations: five plus 2 blank controls Incubation Temperature: 37 + 1C ETolteamlesnutsBpaesnisd:edMiscorloibdisalodnedhayydorfotgeesntaisneg:acti1v0i.t6y g/L (TSS). by formation of formazan. Incubation time: 15-minutes. 001622 RESULTS Nominal concentrations: Blank control, 10, 100, 1,000, 10,000, and 100,000 mg/L. Element values: Not given. Conclusions 00000000000 Nproepcaorncelduisnioonrdsegrivteond.etNeormtirniephmeonyllefsoromfazTaFn p(rTFo)duscteadndianrtdhecutrevste swoalustions, therefore, a plot of percent inhibition vs. log concentrations was not constructed to determine the EC50 value based on dehydrogenase activity. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 Dm ATAQm UALITm Y. mmmm-------- Reliability: Kiimisch ranking 3. Study lacks description and recordsofthe method followed. The inoculum is not properly characterized. There is no cchoanrfaicrtmeartiizoantioofntohfetahmeotuensttosfufblsutoarnoccehepmuriictaylapnrdoptohretisotnudiyn tlhaeckssolauntailoyntsi.cal REFERENCES `The studies were conducted by the 3M Company, Environmental Laboratory, Lab Request Number 4970S, 1978. OTHER Last changed: 6/26/00 001623 rr BEST COPY AVAILABLE me ge 44205 SAMPLE DESCRIPTION EC -206A ece-%8-22 ENVIRONMENTAL ENGR. LAB -- WORK SHEET Tv [App2eis Analyst: a 00000 f 7 / oy 7 D. v 2 ' STK SoLuTIO 22.04 e-200 Alutid 4p (00d. wre Dol urate [=e 22k 0g) wedDlee, D nd oa 0 r, Descrplics) |Toutwitnil|Povtao] mpddigit] TTC | I60) |ot|Gt"[Gd) [nt Bonk *| |soicaot|tce om|jo|| 0 malt |sort oa| ta sose|z jo|| yi 00 losor5s|gsroa[arto|| yi 000 (Comt0di|bsto r[ orro|| u 0,000 It 00,000 1 Blank *2 |max |S86 |so [to [| The dilitirrs se iinzatated aft 37%1C fou pps {Sue iow, 001624 Fie: poe, 4920 S ENVIRONMENTAL ENGR. LAB --WORKSHEET `SAMPLE DESCRIPTION Fe-206 A A Oa: E AWnaaiya: 00000000 Jr0000000 Aibrs, I" ri zat 02 dol) S~ : Afr Ihe vend 4 rn woe ~ Ll. [ / tbat of +L hopl aval v -- Reedy: 001625. BESTCOPYAVAILABLE = Se 44705 ENVIRONMENTAL ENGR. LAB -- WORK SHEET SAFMPeL-E 2DE0ScCR4IPTION et 785-22 [A=nyLorie [rr -- - 0000 T 0 c 000000 Massed igus oun Pogo = Mg Sewngs Plas plindy 2 ale. aK nity ganae XT Heo ~o74Gi, cvatoatitle. o|Ewnaa tmiassn=J1d8,600 0m) 10 and ic RestCemgepd) 0000 nltntatori of Samp / amamella Blok *\(epdul) 0 |50.0% x 00 42.9 1000 34.0 0,000 44.3 00,020 2100 Blanka (Wid) 50.0 Dondiadds past, ethanol [anh had) lands, Fle Keagli Hof Bla%nfe #| af Hla s7ully wa0 aS07,7T707 teach " Blank *| Aq Hunn oT fst 0 AeteLine boo LoniZesl. aula, Theo OM ctu the endiainns tle at thee Hod timed a. . v . 901626, . LR 497 IoS F He EERwll rey Y H FE EEE E E Ho ee eS ame o LEEEnEEmEmIHIH E EE EEIHELPEL ER L EEL Hi H bE HRHHEEE a: FEE A FE EE ER re Pe EE SySR E g R E EE (Leee e n C3 Phe FEEAS EEEE ERr AH ARHRPREH H H Ser EEE HERE HE Be iEe GE CH EE E HR H A HHH y E EEE E eee 3 EE aE ee T Bh FH EEEe BE T E immee HE FRE EE EE ee i @ J EE Eee oo SAMPLE concamnTion - g/t 0016274 | Lab hog. do. 4970S raat LIDS ] soairne (4,50.0 NT x oo erSP Calculation: 0/8 Restle = ggsestiun c joce g.e Eno rs. Plat= p*Diapteesdnbagl1)y-2s9-e7o>dF fous | 75s |75s | Jz Tow | 7 Seige a3.T95va2 re2 l|a0 mm2 1on0 s7 Taig |ooze [o|o[ z7 e nn Lrog00 |1ggoo| | | 0 swuespte | Veta Lr wpe | Seige Tr i | TT He wiped Lg wor was allowed fo ptgty hpore es. Ha TTC Awot. *% Aernolin+g Jum WopkoHo ot Mk LSSs an, Ha cothile <a wash, hm lon sks Me i