Document r91e4ZMw6rKOY88QzmNBMYJJ

AR R26 0787 ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) IESTSUBSTANCE ~~~ 0000 Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206 or L- 3243. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-206, identified by the laboratory as "Sample C". Current information indicates it is a mixture of 0.67% PFOS, 17.5% diethylene glycol butyl ether, 78.91% water, 1.33% Sultone foamer, 1% sodium octyl sulfate, 0.04% sodium lauryl sulfate, 0.5% polyoxyethylene monooctylphenyl ether, and 0.05% benzotriazole. - The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. M-- ETHOD -- ee ------ ---- -- -- -- Method: Methods for Toxicity Tests with Aquatic Organisms as formulated by the Committee for Toxicity Tests with Aquatic Organisms Type: Acute static GLP: No Year completed: 1974 Species: Daphnia magna Supplier: In-house cultures maintained at Bionomics, EG&G, Inc., `Wareham, MA. Analytical monitoring: Not given. Exposure period: 48-hours Test organism age: 12 - 24 hours old Statistical method: TLs, (median tolerance limit) values calculated using a linear regression equation. = Test conditions: Dilution water: Well water, aged 24 hours Dilution water chemistry: Not given Lighting: Not given Stock and test solution preparation: Not given. Exposure vessels: 250 mL beakers with 200 mL exposure solution Number of replicates: 4 Numberof daphnids per replicate: 5 Number of concentrations: five plus a blank control Food: Food, consisting of a homogenization of starter trout food and Cerophyl in water, was added at rate of 0.5 mU/L after introduction of test substance. Water chemistry during the study: 001507 `Temperature range (048 hours): 20+ 1C RESwts 00000 Nominal concentrations: Blank control, 3200, 4200, 5600, 7500, and 10000 mglL. Element values: 48-hour TLso = 5650 (4810 - 7110) mg/L. 48-hour NOEL = 3200 mg/L. Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. - conclusions 000000 TcohnefiFdCe-n2c0e6in4t8e-rhvaolurofT4Le8o10watos determined 7110 mg/L. to be 5850 mg/L with a 95% The 48-hour no observed effect level (NOEL) was 3200 mg/L. Submitter: 3M Company, Paul, Minnesota, 55133 Environmental Laboratory, P.O. Box 33331, St. pataQuaury Reliability: Kiimisch ranking = 2. This study meets the criteria for quality tsetsutdiyngl.acHkoswaenvaelryt,ictahlecsoanmfiprlmeatpiuorniotyfwtahse anomtouprnotpeorflfylucohraorcahcetrenriiczaeldparonpdortthieon in the solution. R0 EFER0 ENC0 ES 0000000000 Test was conducted by Bionomics, EG&G, request of the 3M Company, St. Paul, MN, 1In9c7.4,.of Wareham, MA at the - Q-- UEER--e --e--s-------------- Last changed: 6/28/00. 001508 3 BIOASSAY REPORT SUBMITTED TO 3M COMPANY ST. PAUL, MINNESOTA # ACUTE TOXICITY OF SAMPLE C TO WATER FLEA (Dephnia magna), AND SCUD (Gammarus fasciatus). EBiGon&oGmi,cs Inc, J E79n0virMaoinnmenSttarleetConsultants AWuagrueshta,m, 1M9a74ssachusetts 001509 ' METHODS AND MATERIALS ' . These investigations were performed at the aquatic toxicology laboratory of Bionomics, E G & G, Inc., Wareham, Massachusetts. The susceptibility of water flea (Daphnia magna), and scud (Gammarus fasciatus) to Sample C, a brown liquid, was evaluated under static bioassay conditions for a h8-hour period, Results were expressed as the median tolerance limit (TLgy), the nominal concentration of the test compound in water causing 50 percent immobilization for the Daphnia and Gammarus. The TLg, value and its 95% confidence intervals were calculated by converting the test concentrations and the corresponding observed percent ) mortalities to logs and probits, respectively. These values were then used to calculate a linear regression equation. v Procedures for static bioassays as described in the Methods for Toxicity Tests with Aquatic Organisms as formulated by the Committee for Toxicity Tests with Aquatic Organisms were followed, except for those conditions described below. The Daphnia, and Gammarus were acquired from laboratory cultures. The Daphnia and Gammarus bioassays were conducted at J 20C (1.0). .The invertebrate tests were conducted in 001510 . Page two 3 250 ml beakers containing approximately 200 ml of well water which was aged for 24 hours. Five 12-24 hour old Daphnia, and five fourth instar Gammarus were placed in each beaker (4 replicates/concentration). In the Dephnia bioassays, Daphnia food was added ata rate of 0.5 ml/liter after the introduction of the compound into the test vessels. RESULTS The predicted TL, values and 95% confidence intervals for the Dephnia and Gdmmarus are presented in Table 1. Table 2 presents a summary of observed immobilization ) for each individual test after 24 and 48 hours. : > ; . ----e --e ---------------------------------- Food was prepared by homogenizing 5 g of starter trout 00d and 1 g of cerophyl in 100 ml of water. 001511 y - SUBMITTED BY: EBiGon&oGmi,cs Inc. E79n0virMoanimnenSttarleetConsultants WAuagrueshta,m, 19M7a4ssachusetts PREPARED BY: ) Robert E. Bentley . CIhniveefs,tigAactuitoensToxic: APPROVED BY: Bevier Hasbrouck Sleight, ITT Director, Freshwater Programs 7 001512 Table 1 -- Acute toxicity of Sample C to Daphnia and 3 Gammarus." `The data are based on results of bioassays conducted at the fish toxicology laboratory of Bionomics; E G & G, Inc., Wareham, Massachusetts. -_-- Species TLgo-Milligram active ingredient/liter how "FE our - No Effect e(vmee/l1) . Daphnia sioj660 5,850(4,810-7,110) 3,200 Gammarus --- 5,310(%,340-6,510) 5,170(%,190-6,370) 3,200 . Bioassay conducted at 20C (+ 1.0), Daphnia 12-24 hours old ) : at the initiation of tests. PBioassays conducted at 20C (4 1.0) Gammarus 12-24 hours olq at the initiation of tests. * Co 95% confidence intervals. 2) : 001513 5S Table 2 -- Concentrations tested and observed percent immobilization for Daphnia and Gammarus exposed : lo Sample C after 24 and 48 hours. -_-- Species -_-- Concentration (mg/1) immobilization observe % mobilisation obgerve ! Daphnia 10,000 ~ 0 100 7,500 ; 25 50 | 5,600 . Lo 4,200 o 10 3,200 o 3 control o o Gammarus -_-- 10,000 7,500 | 5,600 4,200 3,200 control 100 80 T 60 us >o o 100 a 75 50 _0 ;o . 001514