Document r7yL1DmjLXKadd01vQnq3Qd7

ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatmea;y also be referredtoas PFOS, FC-95, oras a component ofFC-203C and L-5440. (I-Octanesulfoniaccid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafploutoarsos-i,um salt,CAS # 2795-39-3) Remarks: The 3M productionlotnumber was 501. The testsample is L-5440. Currentinformatioinndicatesitisa mixtureof 1.47% test substance,60% water,30% diethyleneglycolbutylether,3% sodium octyl sulfate3,% hydroxyfoamer, 10% polyoxyethylenemonooctylphenylether, 0.12% sodium laurylsulfatea,nd 0.05% benzotdazol. The followingsummary appliesto the testsample as a mixture of the testsubstance inwater solutionwith incompletely characterized concentradon of impudties. Data may not accuratelyrelatetoxicity of the testsample with thatof the fluorochemical component. METHOD: Method: 3M Protocol,Reference EAR 11/8f79 Test type: Staticacute GLP: No Year Completed: 1981 Analyticalmonitoring: Dissolvedoxygen concentrabons Statisticamlethods: Graphed Test organism source: Activatedsludgemixed liquocrollectefdrom the Metro Wastewater Treatment Plant,St.Paul,MN Test condition: Dilutionwater: Deionizedwater. Syntheticsewage: Not used. Reference and testsolutionpreparation: A stocksolutionofthe testsubstance was createdby dilutin0g.60 grams oftestsample to 100 mL withdilutiownater. Itisnot noted exactlyhow the 1,000 mg/L testsoluton isprepared from thisstocksolution. Test vessels: Not noted Number of concentrations:1 plus 1 blank control Temperature: Not noted Total suspended solidsand pH on day of testing:Not noted Element Basis: Respirationinhibitonas determined by oxygen consumption. RESULTS Nominal concentrations:Blank controland 1,000rng/L Element values,. No acute inhibitoreyffecton acbvated sludge respiratiornateat 1000 mg/L witha contacttime of 6 minutes. Element values based on nominal concentrations. Remarks: Testingwas conducted on the mixtureofthe testsubstance as describedinthe testsubstance remarks field.The values reportedapply tothatmixtureand not thefluorochemicalproportionalone. CONCLUSIONS The testmaterialexhibitedno inhibitoreyffectat 1000 nig/Lover a 6minute exposure. Submitter: 3M Company, Environmental Laboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY Reliability:Klimischranking3.Testinglacksrecordof methodology used. The method was notan agency approved method. Test duration was not longenough todefinitivedleytermineiftheremight be other adverse effectsthatmight occur duringtypicalwastewater treatment retentiontimes. Sample puritywas notcharacterizedand the studylacks analyticaclonfirmationofthe amount offluorochemicalproportioninthe solution. REFERENCES This studywas conducted by the 3M EnvironmentalLaboratory,St Paul, MN, Lab Request number 6811 S, 1981. OTHER Last changed: 6/26100 . SLUDGE .1 .-I@- - TOX, RESULTS i cl E Cf- 5 z L0L) 4 C) ri 2 3M Environmental Laboratory (Acute Toxicity of a Compound to Activated Sludge Mixed Liquor) Simple Description: Analyst: 4. Date Analyzed: tyolcc Slel-?f-@f@3 Lab Request No.: C0811 s --------- --- III L ------ ---- ----------- oo-- r 7 7 7 77.... TIME minutes 10 12 3M Environmental Laboratory Acute Toxicity of a Compound (Substance) to-Activated Sludge Mixed Liquor Protocol Reference: EAR 11/8/79 Lab request no.: Date: Ana Iys t The ac tivated s ludge mixed Ilquor was col lacted from the St.. Paul/Mi nneapol is Metro Wastewater Plant on 7-01- ef Sample description: 9141-?afi Ft@4 i(21 L 0'7-*- Preparation of stock solution: 0.60 grams of.swole were diluted to 1171D mi with deionized reagent water. 0-afe--o-f-preparatioZn-:O/-Fit -YiME min. sec OKC N m ION 050;EST Mf4ERIA IN-ASTIVIAI cm m I mg@l mq/1 mg/l L12-a@t-q-ol Is SL GE RE 0!@l m 30 7,-/,r7,OD (0,00 os-, /0 COMMENTS: