Document qmwbnkbEa4yk3eB0qgdevK4Dj

ARID6-033% ACUTE TOXICITY TO AQUATIC INVERTEBRATES -- Daphnia magna TEST SUBSTANCE Identity: N-Ethylperfluorooctanesulfonamide; may also be referred to as OUc1t4a6n4e,suElFfOonSaAm,idEet,P1O,S1.A2,.2F,-36,33,049,,4.o5r,5F.X6-.162..7.7(.18-,8,8heptadecafluoro-N-ethyl-, CAS # 4151-50-2) Remarks: Material is a white solid. Sample purity and lot number were not recorded. METHOD Method: Adapted from OECD 202 -- Water accommodated fractions Type: Static acute GLP: Year cNomopleted: 1998 ASnpaelcyiteisc:alDampohnintioarminagg:naNominal concentrations only. Temperature, pH aStnadtidsitsiscoallvmedetohxoydgse:n recorded : EL (lethal loading) values were calculated using tThOeXpSroTbAitTmSeotfhtowda.reTPhaecNkaOgEeL, wVearssicoanlc3u.l5a,tLeadrbaymiFei,shWeyr'osmiEnxagc.t Test, Test daphnid source:. AScl in-house culture, Duluth, MN Test daphnid age at study initiation: < 24-hour old neonates Test coDnidliuttiioonnsw:ater: Carbon-treated dechlorinated City of Duluth, MN tap water aerated for 24-hours prior to use in the test. Dilution water chemistry during the study: Hardness 44 mg/L as CaCOs AlDkOal:inity: 8.1-8.9 mg/L 42 mg/L as CaCO3 CpoHn:ductivity 7.982 113-125 umhos/em Test Temp. Range: 21.3-21.5 Stock and test solution preparation: Test solutions prepared by `(moansespaedrdicotnicoennotfratteisotns).ubsTthaencseoltuoti2o.n0sLwaelriqeuovtisgoorfoduislluytisotnirwraetder (vortex 1/3 to % solution depth) for 18.5 hours. The aqueous phase was siphoned from the vessel at mid-depth. Exposure vessels: 250 mL borosilicate glass beakers 006049 Exposure volume: 200 mL Number of replicates: four Number of daphnids per replicate: 5 Numberof concentrations: Five plus a negative control Element basis: mortality RESULTS Nominal loading concentrations: Bk control, 5.8, 9.6, 16, 27, and 45 mg/L Element value: 24-hour ELso: 24.6 (21.3-28.6) mg/L 48-hourELso: 14.5 (12.1-17.4)mg/L. 48-hour EL+o: 7.3 (5.09.2) mg/L 48-hour NOEL: 5.8 mg/L Mortality of controls: 0% after 48-hours exposure ET Percent Mortality i] [Roa Gomer[00 [ore [eToT=| Fr > [7Tes] = Tm] Remarks: All values reported are for nominal loading concentrations. No measurement of test concentrations was conducted. CONCLUSIONS 00000000 `The test sample 48-hour ELs for Daphnia magna was determined to be 14.5 mg/L with a 95% confidence interval of 12.1 -- 17.4 mg/L Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking 2. This study, while well conducted using the methodology acceptable at the time, lacks analytical data for determination of the test substance concentration in the test solutions and determination of the sample purity. Testing was also done well above the 006050 solubility limit of the test substance with the use of water accommodated fractions. REFERENCES `Study conducted at the request of 3M Company by, AScl Corporation, Duluth, MN. O0 THER OOOO Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 Last changed: 5/18/00 0069S LTMs i: EAnSveirlonCmoernptoarlaiToens/tAiSncglD-iDuvliustohn `SuXbmSiteSdut0d3IMD#Co3.01-0509688 STUDYTITLE ACUTE TOXICITY OF U1464 TO DAPHNIA MAGNA DATASTANDARD `OECD GUIDELINE 202 STUDY COMPLETED : May 20, 1998 TESTINGFACILITY AScl Corporation/AScl-Duluth `Environmental Testing Division 4444 Airpark Boulevard Duluth, MN 55811-5712 Tel. No. (218) 722-4040 Fax No. (218) 722-2592 STUDY IDENTIFICATION NUMBERS AScl Study ID# 5010-068 3M Company Lab Request# U1464 : : SpSopnonssoorr:Su3dMy CIoFmpLan4y64 1 006052 . } - EAnSveilroCnomrepnoaaltiToens/tAiSncgl-DDiuvliustonn `S"uAbSmeilaaSdutdoy3[MD4Co0.10-.505688 1.0 GENERAL INFORMATION [es er Study Title "Acute Toxicity of U1464 to Daphnia magna_. ] [waswnawa [oBCD Guideline 202 | Sponsor 3M Environmental Technology and Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, I MN 55133. | | `Sponsor's Representative . Rochelle R. Technology aRnodbiSdeeravui,ce3s,MBuEinlvdiirnognm2e-n3t6a-l09, | | 935 Bush Avenue, St. Paul, MN 55133. Tel No. (612) 778-7065. | { sponsor's Lab Request Ul464. I Testing Facility I T`eAsStIinCgorDpiovirsaitoino,n/4A4S4c4l-ADiurlpuatrhk EBnovuilreovnamredn,tal I Duluth, MN SSB11; Tel. No. (218) 722-4040. | [Principal Investigator Todd McGuire. |I Testing Facility Director { Definitive Test Dates Data Validation and Report Review Donald Mount. April 28-30, 1998. `Alan Mozol. 50 | Retention of Raw Data and Final Report I Location of Raw Data and Final Report L yang { `Two years from the date study is completed. T`AeSsctlinCgorDpiovriasitoino,n/4A4S4c4l-ADiurlpuatrhkEBnovuilreovnamredn,tal || Duluth, MN 55811; Tel. No. (218) 722-4040. | SSptonasnors:Su3dMy CToFmpDarnash 2 006053 ?n EAnSveilroCnmoeroguoarlniToen/nAgSclD-iDvuilsiohn , Su"bAmSielteSdtutdoy3DMFCo5.01-0.$0688 2.0 OBIECTIVE(S) { To determine the 48-h EL's (effective loading's) ELyg and ELsq, and 48-h NOEL (no observed | | effect loading) of the test substance, U1464, for Daphnia magna. | {|| TGhuiisdesltiundey2w0a2s(cOoEndCuDct1e9d93a)c.corTdeisntg stoolutthieonASpcrelpasrtautdiyonplwaansrewtaatienesd-abcycotmhemoSdpaotnesdorfraanctdioOnECD (WAF) per Girling & Whale 1994. _ J 3.0 TEST METHODS - | "Test Substance i `Test Organisms I t [Dilution Water { I . || ECxhpaomsbuerres Parnodpenretgileisg:iblUe1s4o6l4ub,ilwihtyitien cwraytsetarl.s, specific gravity 1.7 (water = 1), stable, |l Test loadings: 0 (dilution water control), 5.8, 9.6, 16, 27, and 45 mg/L. | `Each test solution prepared aliquots of dilution water. by mass addition of test substance to 2.0-L Each solution was vigorously stirred (vortex 1/3 to 1/2 of flasks' depth) for 18.5 hours to obtain WAF's. Test solution I obtained from flasks by siphoning aqueous phase from mid-depth. | water. | Source: Healthy, < 24-h old Daphnia magna neonates obtained from AScl `mass organism cultures maintained in dechlorinated City of Duluth tap Use in test: Gravid adults isolated 24 h before test initiation. Used 5 { otersgta.nisms/replicate and 4 replicates/test loading. Did not feed during the | Source: Carbon-treated dechlorinated City of Duluth, MN, tap water. The | `Pmaocsktagreec.ent chemical analysis of post carbon (PC) water is in the Raw Data | Characteristics: At test time, the water had a hardness of 44 mg/L (ss | CaCO) and a pH of 7.99. Aerated for 24 h prior to using in the test. { Type: 250-ml borosilicate glass beakers. Use in test: 200 ml of dilution water or test solution/chamber. During the test, kept covered, except when experimental observations were made. . SSpponosoorr: Su3dMy C1oDm#pUanGy H 3 C6o5a `. EAnsvc`i]SruoCbnommrieptnoetrdasttoiTeo3snMt/iAnSCgeo.lD-iD-vuil5su9ot8nh - "ASe Sway IDI 5010068 ll Observations, | Endpoint | Catcuations | Duration: 48 h. l Daily photoperiod: 16 h light and 8 h dark, maintained using cool-white fluorescent bulbs. Temperature: 21C. Biological: Daily- counted dead test organisms. Did not remove any | daphnids from the exposure chambers at 24 h. Water chemistry: (1) At test initiation- total hardness and alkalinity, (2) daily- temperature, pH, DO, and conductivity. All determinations were made according to APHA methods (1995). ELyq and ELs;: EPA Probit Method. | NOEL: Fisher's Exact Test (TOXSTAT statistical software, Version 3.5, University of Wyoming & West Inc.). | 3.0 RESULTS Test Substance Toxicity (95% Confidence Interval) Pertinent Data are in Table 1 l | 24h ELgy: 24.6 mg/L (21.3-28.6 mg/L). 48h ELgy: 14.5 mg/L (12.1-17.4 mg/L). I 48h EL: 7.3 mg/L (5.09.2 mg/L). | I RangesofWater Chemistry Parameters 48-h NOEL: 5.8 mg/L. Total hardness (as CaCO; mg/L): 44. Alkalinity (as CaCO mg/L): 42. | | Temperature (C): 21.321.5C. DO (mg/L): 8.1-8.9. I pH: 7.982. _ | Specificconductivi os/em): 113-125. | SSppoonnssoorr:S3iMd C1o0m3pUan1y464 4 006055 +. EAnSveilroCnmoernptaarltTieosnt/iAnSgcDli-vDiuslohn - `Su"bAmSieteSdatoy31MD#CSo.o1-0509688 4.0 TEST QA/QC [T wom om] --_-- ---- ~~~ a------------ Test organism dead, immobilized or trapped in| 0% dead, immobilized or trapped. control exposure must not be more than 10%. |1 I The lowest DO concentration at test termination must be at least 60% of the air saturation value at test temperature. "The lowest DO concentration measured was 91% of the air saturation value at 21.0C. "Test duration must be 48 h. `The test duration was 48 h. I Reference substance toxicity test (this testis | Current control chart for D. magna acute l | optional). testing is included in the raw data package. 5.0 REFERENCES 1 American Public Health Association (APHA). 1995. Standard Methods for the Examination of | `Water and Wastewater. APHA, Washington, D.C. Girling, A.E., G.F. Whale, and D.M.M. Adema. 1994. A Guideline Supplement for I | DAectceormmmionidnagtetdheFrAaqcutaitoincs.ToCxhiecimtoyspohfeProeo,rlVyolW.at2e9r,-SNool.ub1l2e, Cppo.mp2l6e4x5-M2i6x4t9u.res using Water- l | Organization for Economic Cooperation and Development (OECD). 1993. OECD Guidelines for Testing of Chemicals. OECD Publication Information Center, Washington, D.C. l University of Wyoming & West Inc. 1996. Toxstat Version 3.5. Cheyenne, Wyoming 82001. | USEPA Probit Analysis Program. Version 1.4. _ 006056 SSppoonnssoorr. Su3dMy CIoDmFpUan4y64 5 A - - RESTO0PY AVAWLABLE i Sy 108 3910068 ASel Corporation/AScl-Duluth Envi`rSounbmmeintetdalioTe3sMtinCgo.Div-isSi5on8. TABLE 1. ACUTE TOXICITY OF U1464 TO DAPHNIA MAGNA ie remo] 1 Eos lac eehn EE we D= L = ] Fre e0 T Te | TT we ie | * Percentages are in parenthesis. : EFFECT AND NO-EFFECT LOADINGS WITH `CORRESPONDING 95% CONFIDENCE INTERVALS w wemommy,,[ s w | ] e meeansme]| wee, mT sem | SSpponosoorr: SIyM C1o0m8pOandyes . 006057