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I'-- NEW YORK UNIVERSITY MEDICAL CENTER A private university in the public service - Institute of Environmental Medicine 550 flRST AVENUE, NEW YORK, N.Y. 10016 AREA 212 679-3200 PLEASE REPLY TO: NEW YORK UNIVERSITY MEDICAL CENTER BOX 817, TUXEDO, NEW YORK 10987 May 18, 1979 Mr. Clayton F. Callis, Director Environmental Operations & Tech. Monsanto Company 800 N. Lindbergh Blvd., B2SA St. Louis MO 63166 Planning Dear Clayton: . I am enclosing the proposal prepared by Dr. Lee for support of his investigations of the rates of degradation of PCBs by microorganisms normally present in the aquatic environment. We propose that support be provided for three years at a total cost of $154,931. As indicated in the proposal. Dr. Lee will serve as principal investigator, assisted as'needed by Drs. Kneip and O'Connor. . We appreciate the interest and confidence you have expressed in our program, and we look forward to receiving the contractual document from you. As I am not familiar with the form of the contracts you offer universities, I should mention that university policy requires that Dr. Lee be given complete freedom as principal investigator, within the proposed scope of work, and that there be no restrictions on his publication privileges. ME:emc Enclosure Professor and Director Laboratory for Environ mental Studies PCB-ARCH-EXT0376768 PROPOSAL TO MONSANTO COMPANY TITLE: The Degradation of Polychlorinated Biphenyls by Fungi and Anaerobic Bacteria PRINCIPAL INVESTIGATOR: Dr. Chun C. Lee (SSN 521-52-6704) INSTITUTION: / New York University Medical Center Institute of Environmental Medicine Laboratory for Environmental Studies 550 First Avenue New York, New York 10016 DURATION OF PROPOSED WORK: June 1, 1979 through May 31, 1982 TOTAL AMOUNT REQUESTED: $154,931 ---- - Chun C. Lee Research Assistant Professor Institute of Environmental Medicine New York University Medical Center Tuxedo, New York 10987 Institute of Environmental Medicine New York University Medical Center Tuxedo, New York 10987 ' Institute of Environmental Medicine New York University Medical Center . 550 First Avenue New York, New York 10016 Thoma's^jW'^itzgerald, Director Office of Grants Administration and Institutional Studies 550 First Avenue New York, New York 10016 PCB-ARCH-EXT0376769 OBJECTIVES This research will seek to isolate and cultivate fungi and anaerobic bacteria from a variety of aquatic environ ments, which are able to grow in the presence of polychlorinated biphenyls and degrade the polychlorinated biphenyls. After such cultures have been established, the rate and mode of polychlorinated biphenyl degradation by these cultures will be examined, both in vitro INTRODUCTION This study is proposed to investigate the role of fungi and anaerobic bacteria in polychlorinated biphenyl (PCB)degradation. Because of their low solubility in water, high specific gravity, and high adsorbability, PCBs in aquatic environments tend to accumulate in reducing or anaerobic sediments of lakes, rivers, estuaries and coastal zones (3,4,9) . Evidence available to date indicates that microbial degradation may be a mechanism by which PCBs are eliminated* from the environment (1,5,7,8,10,11,13). PCB resistant or degrading bacteria have been found to be ubiquitously distri buted in the natural environment (11). Thus far, degradation studies have centered on aerobic bacterial activity, and are not applicable, directly, to PCBs found in reducing or anaerobic sediments. Little information is available on the role of fungi. representative sites in freshwater, estuarine and marine environments. These samples will be collected by the principal investigator or by requests of colleagues at other institutions. Physical and chemical aspects of the environment at the time of sampling (e.g., salinity, depth, pH, Eh, water temperature) will be recorded when possible. Immediately upon collection, the sediment will be transferred to duplicate sets of glass bottles which will be almost filled with filtered water and sealed with serum caps. One set will be gassed with nitrogen to maintain or to establish reducing conditions necessary for anaerobes; the other will be maintained aerobic for fungal isolation. Both sets of samples will be used for subsequent isolation of PCB degraders by enrichment culture. Enrichment cultures for anaerobic bacteria will be set up by adding 1-2 g of sediment to a 250 m flask containing 100 mil of an organic medium (thioglycollate medium) made with distilled water or water of appropriate salinity and pH for estuarine and marine samples. Techniques for anaerobic cultures as described by Bryant (2) will be strictly followed. Cultures will be incubated for three days to two weeks at 26C; intermittently, culture flasks will be shaken by hand. Isolation will be carried out in a second flask which contains 100 mil of a mineral salt solution adjusted to the desired salinity: (NH4)2S04, 1 g; KH2PC>4, 0.2 g; K2HPC>4, 1.6 g; MgS04*7H20, 0.2 g; NaCl, 01. g; FeS04-7H20, 0.01 g; CaCl2*2H20, 0.02 g (pH 7.5) containing 0.05% of the appropriate PCB. Following standard anaerobic techniques, 1 mil of the enrich 3 PCB-ARCH-EXT0376772 ment culture will be transferred to this second medium and incubated as before until substantial growth is observed. Such cultures will be maintained in duplicate or triplicate. One series will be incubated in the light and one in the dark to enable the growth of phototrophic bacteria as well as strict chemotrophs and heterotrophs. , Enrichment cultures for fungi will be started in Sabouraud dextrose agar (Difco) made to the desired salinity and incubated under aerobic conditions. After initial growth, 1 m of the enrichment culture will be transferred to the PCB- mineral salts medium (described above) but amended with 1 mil of a trace elements solution per liter. The trace elements solution contains 0.1 g H^BO^, 0.05 g each of CuS0^*5 H^O, MnS0^H20, ZnSO^-7 H20, Na2MoO^, and CoC12*6 H20 per liter. After incubation for predetermined time intervals (4 days to 1 month), aliquots of the incubated samples will be examined by high performance liquid chromatographic techniques, .or they will be transferred to separatory funnels and extracted with hexane for gas chromatographic analysis. Gas chromato grams will be run with 1-5 yl aliquots of the hexane extracts. Initially, only the disappearance of certain PCB homologs from the mixed culture systems will be measured. If the PCBs disappear or decrease in quantity, degradation products will be looked for, perhaps using thin-layer chromatographic 14 techniques and with C-labelled PCBs. Mixed cultures will be purified by plating onto mineral salts agar plates containing 0.05% PCBs; degradation studies will be conducted with the resultant pure cultures. ' 4 PCB-ARCH-EXT0376773 2. Procedures for Specific Aim 2: Once the respective microbial systems capable of growth on PCBs are obtained, the kinetics of PCB degradation will be followed by analyzing the PCBs in the growth medium at.different time intervals. The cells will be harvested by centrifugation and washed twice with 0.5 M phosphate buffer (pH 7.0). The washed cells will be injected into culture tubes containing the mineral salts medium a.nd PCB. The procedures employed will be similar to those described by Halvorson et al. (6), who used resting suspensions of bacteria harvested from a sewage lagoon to examine the degradation of mixtures of pesticides. Comparisons will be made of gas chromatograms of PCBs before and after incubation with test organisms and with non-inoculated PCB controls over the pre-determined time interval. Metabolites and residual PCBs will be characterized by the use of column and thin-layer chromatographic techniques of Stalling and Huckins (12), and Furukawa and Matsumura (5). 3. Procedures for Specific Aim 3: In situ studies will be conducted with wa-t-m-ai codimentg whose PCB content will have been determined previously. Sediments will be autoclaved to kill indigenous microbial flora. Depending on the test organ isms to be used in the test (fungi or anaerobic bacteria), the test conditions of the sediment will either be made anaerobic or left aerobic. The specific organism for the PCB degrada tion experiments will be inoculated into the sediments, and the rest of the experiment will be run as in Procedure for Specific Aim 2 to determine PCB degradation. A second variation of the 5 PCB-ARCH-EXT0376774 in situ study involves the inoculation of the test isolates into non-sterilized sediment of known PCB content along with all of its indigenous flora and fauna; this is designed to test the ability of the isolated PCB degraders to function and compete with other organisms under natural conditions. PCB degradation will be assessed as per Procedure for Specific Aim 2. 4. Th6 research tasks will be performed in the following sequence, assuming a beginning date of June 1, 1979: June 1, 1979 - May 31, 1980: Specific Aim 1. June 1, 1980 - May 31, 1981: Specific Aim 2. June 1, 1981 - May 31, 1982: Specific Aim 3. 6 PCB-ARCH-EXT0376775 LITERATURE REFERENCES 1. Ahmed, M. and D.D. Focht. (1973). "Degradation of poly chlorinated biphenyls by two species of Achromobacter,11 Can. J. Microbiol. 19: 47. 2. Bryant, M.P. (1972). "Commentary on the Hungate technique for culture of anaerobic bacteria," Amer. J. Clin. Nutrition 25: 1324. 3. Duke, T.W., J.I. Lowe, and A.I. Wilson. (1970). "PCB (Aroclor 1254) in the water, sediment, and biota in Escambia Bay, Florida," Bull. Environ. Contamin. Toxicol. _5: 171. 4. Fulk, R., D. Gruber, and R. Wullschleger. (1976). "Labora tory study of the release of pesticide and PCB materials to the water column during dredging and disposal operations," Final Rept. of U.S. Army Engineer Contract No. DACW39-74- COl42. 5. Furukawa, K. and F. Matsumura. (1976). "Microbial meta bolism of polychlorinated biphenyl components by Alkaligenes sp.," J. Agri. Food Chem. 24_: 251. 6. Halvorson, H., M. Ishaque, J. Solomon, and O.W. Grussendorf. (1971). "A biodegradability test for insecticides," Can. J. Microbiol. 17: 585. 7. Kaiser, K. and P. Wong. (1974). "Bacterial degradation of polychlorinated biphenyls. Identification of some metabolic products from Aroclor 1242," Bull. Environ. Contamin. Toxicol. 11: 291. i r 8. Liu, D. (1976). "PCB degradation," Mar. Pollute Bull. 7_: 83. 9. Munson, T.O., H.D. Palmer, and S.M. Forns. (1976). "Trans port of chlorinated hydrocarbons in the Upper Chesapeake Bay," Nat11 Conf. on Polychlorinated Biphenyls, Chicago, 111., p. 218. 10. Sayler, G.S., M. Sbon and R.R. Colwell. (1977). "Growth of an estuarine Pseudomonas sp. on polychlorinated biphenyl," J. Microbial Ecol. 3_: 241. 11. Sayler, G.S., R. Thomas and R.R. Colwell. (1978). "Poly chlorinated biphenyl (PCB) degrading bacteria and PCB in estuarine and marine environments," Estuarine and Coastal Marine Science 6: 553. 7 PCB-ARCH-EXT0376776 12. Stalling, D. and J. Huckins. (1973). "Reverse phase thin layer chromatography of some Aroclors, Halowaxes, and pesticides," J.A.0.A.C. 56: 367. 13. Wong, P. and K. Kaiser. (1975). "Bacterial degradation of polychlorinated biphenyls II. Rate studies," Bull. Environ. Contamin. and Toxicol. 13: 249. 8 PCB-ARCH-EXT0376777 FACILITIES AVAILABLE The proposed research will be carried out using the facilities of the analytical chemistry and aquatic ecology sections of the Laboratory for Environmental Studies. The proposed research will be carried out in,.the culture and analytical facilities at the A.J. Lanza Laboratory, Tuxedo, New York, and at the Hudson River field station maintained by the Medical Center at Buchanan, N.Y. NYU boats equipped for collection of samples and certain field analytical procedures are berthed at Stony Point, N.Y.. and operate throughout the open-water months. Dock facilities are available at the Buchanan wet-lab, but only on a temporary basis. Equipment available for this project includes apparatus for: Extraction/concentration of organics Freeze-dry techniques Scintillation spectrometry (low level - beta) Gel electrophoresis Thin-layer chromatography ' Gas-liquid chromatography Respiration studies Water quality analysis Autoradiography Culture facilities and equipment for aerobic and anaerobic microorganisms Cultux'e of aquatic organisms Maintenance of aquatic organisms Computer data analysis 9 PCB-ARCH-EXT0376778 PRINCIPAL INVESTIGATOR ASSURANCE The undersigned agrees to accept responsibility for the scientific and technical conduct of the research project and for provision of required progress reports if a contract is awarded as a result of this application. Date Principal Investigator 10 PCB-ARCH-EXT0376779 PROPOSED BUDGET DEGRADATION OF POLYCHLORINATED BIPHENYLS BY FUNGI AND ANAEROBIC BACTERIA 1. Personnel Name C.C. Lee Title Prin. Invest. Theo. J. Kneip Tech. Advisor J.M. O'Connor Tech. Advisor Research Technician Laboratory Aide Clerical Assistance 197S1-80 % Time Salary 20 4,592- 10 N.C. 10 N.C. 100 12,500 - 50 5,071'. 10 1,200 Year 1980--1981 % Time Salary 20 4,821 10 N.C. 10 N.C. 100 13,125 50 5,325 10 1,260 Total Fringe benefits Overhead $23,363 4,731 12,559/ $24,531 5,029 12,879 Total salaries/benefits $40,653. < $42,439 1981 -82 % Time Salary 20 5,159 10 N.C. 10 N.C. 100 14,044 50 5,698 10 1,348 $26,249 5,447 13,780 $45,476 2. Equipment a. High performance liquid $12,000 -- .chromatograph (partial) b. Shaker table 500 - - c. Incubator - 600 - d. Spectrophotometer - 1,150 - Totals $12,500 $1,750 _ 11 PCB-ARCH-EXT0376780 3. Supplies a. Glassware b. Chemicals c. Gases (N2; CC>2) d. Chromatographic equipment (gases, columns, etc.) 1979-80 $500 500 200 600 > - Miscellaneous Expenses a. Travel to scientific meeting b. Personal travel (collection, etc.) c. Publication costs d. Other costs (Xerox, inter-library loan, service, etc.) $1,800 ' $500 500 500 Totals 1. Salaries 2. Equipment 3. Supplies 4. Miscellaneous $1,500 $40,653 12,500' 1,800 1,500 Total amount requested $56,453 1980-81 1981-82 .$500 ' 500 200 600 $1,800 -- $300 200 400 $900 $500 200 300 500 $1,500 $42,439 1,750 1,800 1,500 $47,489 $500 100 300 300 $1,200 $45,476 900 1,200 $47,576 12 PCB-ARCH-EXT0376781 CURRICULUM VITAE May 1979 Chun Chi Lee (Formerly Known as King Mon Tsue) Date and Place of Birth: Marital Status: Residence: Professional Address: Title: Education: Dissertation Title: Positions Held: Course Offering: (Department of Environmental Medicine and Department of Biology) August 31, 1939 - Hong Kong Married, Two children R.D. #2, Felter Hill Rd. Monroe, New York 10950 New York University Medical Center Institute of Environmental Medicine 550 First Avenue New York, New York 10016 Telephone: (212) 679-3200, Ext, 5276 Research Assistant Professor Laboratory for Environmental Studies 1962 - B.S. in Zoology Colorado State University 1969 - Doctor of Philosophy in Marine Science University of Miami (Florida) The decomposition of organic matter in some shallow water calcareous sediments of Little Black Water Sound, Florida Bay New York University Medical Center Department of Environmental Medicine 1975 - Present Aquatic and Estuarine Microbiology University of Miami (Florida) Rosenstiel School of Marine and Atmospheric Science, 1969-1975 Research Scientist, Functional Biology 1969 - 1973 Research Assistant Professor Biology and Living Resources 1973 - 1975 PCB-ARCH-EXT0376782 Positions Held: Professional Societies: Research Interests: Awardsand Grants: University of Miami (Florida) Institute of Marine Science 1962 - 1969 NDEA Fellow, Marine Biology ' 1962 - 1965 " Graduate Assistant 1965 - 1967 Senior Field Biologist McMurdo Sound, Antarctica 1967 - 1968 Senior Field Biologist First International Weddell Oceanographic Expedition 1968 - 1969 ` Sea Colorado State University ,, Department of Botany and Plant Pathology 1958 - 1962 " Research Assistant, 1958 - 1962 American Association for the Advancement of Science American Society of Limnology and Oceanography Microbiology Algal and Protozoan Physiology and Ecology . Biochemistry of Organic Matter in Aquatic Sediments Achievement Award in Biological Science Colorado State University, 1962 National Defense and Educational Act Predoctoral Fellowship, 1962 - 1965 National Science Foundation Grant University of Miami, $44,000 1973 - 1975 National Science Foundation Grant New York University Medical Center, $3,900 ` 1976 PCB-ARCH-EXT0376783 PUBLICATIONS CONT'D. 13. Bunt, J. S,,, C. C. Lee and M. A. Heeb. The site off Freeport intended for Hydro-Lab. Hydro Lab J.,.jL:3-6 (1972). 14. Bunt, J. S,, C. C. Lee. An exploratory study of benthic primary production. Hydro Lab J., ]j7-10 (1972). 15. Taylor, B. F., C. C. Lee and J. S. Bunt. Nitrogen-fixation associated with the -blue-green alga, Trichodesmium, as measured by the acetylene-reduction technique. Archiv fur Mikrobiologie, 88_s 205-212 (1973). 16. Lee, C.C., e. L. Lee and J. S. Bunt. Distribution of biomass in a coral reef transect. In: Results of the Tektite Program, coral reef invertebrates and plants (Sylvia Earle and Robert Lavenberg, editors), Natural History Museum, Los Angeles County, Science Bull., 20^:69-75 (1975). 17. Steinhausler, F., S. Schaffer, N. Cohen, C. C. Lee, J. M. O'Connor and McDonald E. Wrenn. Effect of high LET radiation on intra cellular ATP content of prokaryotic and eukaryotic algae. Radi ation Research Society , 26th Meeting, Toronto, Canada (Abst.). May 14-18, 1978, pp. 591-592. . . . 18. O'Connor, J. M., C. C. Lee and M. Eisenbud. Environmental Health. MESA New York Bight Atlas Monograph 32. (In press). 19. Lee, C. C., R. Califano and R. Sansur. The degradation of poly chlorinated biphenyls (Aroclor 1254) by anaerobic bacteria and fungi from the Hudson River. Atlantic Estuarine Research Society, 61st Annual Meeting, Rehoboth, Del. April 26-28, 1979. PCB-ARCH-EXT0376785 Curriculum Vitae Page 2 Course Offerings: (Department of Environmental Medicine) Analytical Chemistry of Environmental Contaminants Positions Held: (continued) Honor Societies: Professional Societies: Mallinckrodt Chemical Works St. Louis, Missouri 1954-1966 Research Chemist - Uranium Division 1957-1959 Group Leader, Uranium Division 1957-1959 Asst. Manager, Analytical Laboratory Uranium Division 1961-1963 Manager, Laboratory Products Research 1963-1966 Phi Lambda Upsilon, 1950 Sigma Xi, 1954 American Chemical Society American Association for the Advancement of Science American Industrial Hygiene Association American Conference of Governmental Industrial Hygienists 'The New York Academy of Sciences Vice Chair - Environmental Sciences Section 1976 Chair - Environmental Sciences Section 1977-1978 Elected Fellow - 1977 Committees: Chairman of Subcommittee #6 - Metals 1 Intersociety Committee on Manual of Methods for Ambient Air Sampling and Analysis 1971-197.7; Member 1971-present . Member of International Meeting on Effects and Dose-Response Relationships of Toxic Metals, Tokyo, November 18-23, 1974. Organized by: The Subcommittee on Toxicology of Metals of the Permanent Commission and International Association on Occupational Health. PCB-ARCH-EXT0376787 Curriculum Vitae Page 3 Committees: (continued) Consulting: Member of American Society for Testing and Materials Committee D-22: Methods of Sampling and Analysis of Atmospheres, Subcommittees D-22.04, 1973-present. D-22.09, 1975-present. New York State Governor's ad hoc committee on PCB's, 1975. Secretariat, International Standards Organization/Technical Committee 146 (Air Qua!ity)/Working Group 3 Nitrogen Compounds, 1975-1976. Secretariat, International Standards Organization/Technical Committee 2 ' Workplace Atmospheres, 1976-present. - Various corporate organizations including Consolidated Edison, RSR Corporation, , Equitable Environmental Health. United States Department of Commerce, . National Oceanic and Atmospheric Administration, Marine Ecosystems Analysis Project, New York Bight Contaminants Panel Cornell University,.New York State College of Agriculture and Life Sciences and New York State College of Human Ecology, Cooperative Extension, New York City Urban Gardening Program. PCB-ARCH-EXT0376788 Curriculum Vitae Page 4 List of Published Works: ,,1 Laitinen, H. A- and T,, J. Kneip. "Polarographic and Coulometric Study of p-DimethylaminobenzeneJournal of the American Chemical Society, 7_8:736-740 (1956) . 2. King, P., E. Bretz and T. J. Kneip. "Determination of Iodine in Mineral Premixes," Analytical Chemistry, _34:565-567 (1962). 3. Kneip, T. J., T. H. Beaslv, P. King and W. K. Dean. "Programmed Temperature Gas Chromatography with Dual Electron Capture Detector for Pesticide Residue Analysis," Analytical Chemistrv, 39:1510 1512 (1967). . ' - ...........~ * 4o Cohen, N. and T. J. Kneip. "A Method for the Analysis of 210Pb in Urine of Uranium Miners," Journal Health Physics, 17:125-130 (1969). ~ 5^ Strehlow, C. D. and T. J. Kneip. "The Distribution of. Lead * and Zinc in the Human Skeleto'n," Journal American Industrial Hygiene Association, 2K!.:372-378 (1969). 6* v Knein, T. J., G.'P. Howells.and M. E. Wrenn. "Trace Metals, ' P^irTlkr^TTaes and Pesticides in the Hudson River," Symposium on . Hudson River Ecology, October 1969, Proceedings of the Symposium, New York State Department of Environmental Conservation, Albany, New York. ~ 7. Furtado, V. C. , T. J. Kneip and M. Eisenbud. "Measurement of Low Levels of 131j j_n Reactor Atmospheres," Nuclear Applications and . Technology, 9_: 268-273 (1970). . 8. Kneio, T. J., M. Eisenbud, C. D. Strehlow and P. C. Freudenthal. "Airoorne Particulates in Ne rYork City," Journal Air Pollution Control Association, 2_0:144-149 (1970). 9. Howells, G. P.. T. J. Kneip and M. Eisenbud. "Water Quality in Industrial Areas: Profile of a River," Environmental Science and Technology, _4 : 26-35 (1970). .10 Kneip, T. J. "Atomic Abosrption Spectrophotometry," AIHA Confer ence on Advances in Instrumental Method of Analyses, Environmental Trace Substances Center, University of Missouri, Columbia* Missouri ` (May, 1970). ' . . PCB-ARCH-EXT0376789 Curriculum Vitae Page 5 11. Laurer, Gi R., T: J. Kneip. R. E. Albert and F. S. Kent. "In Situ Determination cf Lead on Painted Surfaces for the Prevention of Childhood Lead Poisoning," Proceedings of the Third Svraoosium on Low Energy X- and Gamma Ray Sources and Applications, Boston, Gordon and Breach Science Publishers, New York, pp. 289-301 (1971).. 12. . Lentsch, J. W. , M. E. Wrenn, T. J. Kneip and M. Eisenbud. "Manmade Radionuclides in the Hudson River Estuary," Proc. 5th Annual Midyear Topical Symposium, Health Physics Society, Idaho Falls, Idaho, p. 499 (1970). 13. Howells, G. P., M. Eisenbud and T. J. Kneip. "Ecology of the Estuary of the Lower Hudson River," Proc. FAQ Tech. Conf. on Marine Pollution and its Effects on Living Resources and Fishing, Publ. FIR:MP/70/E-20, Food and Agriculture Organization of the ' United Nations, New York (1970). 14. Kneip, T. J, "Concentration Factors, Environmental Complexity and the Analytical Chemist," American Chemical Society, Analytical Division--National Bureau of Standards Summer Symposium: Key to Progress in National Problem Areas, Washington, D. C. June 1971, Proceedings of the Symposium 15. Kne.ip, T. J.,, G. P. Howells and M. E. Wrenn. "Trace Elements, Radionuclides and Pesticide Residues in the Hudson River," Proc. FAQ Tech. Conf. on Marine Pollution and its Effects on Living Resources and Fishing, Publ. FIR: MP/7 0/E- 2 0, Food arid Agriculture Organization of the United Nations, New York (1970). 16. . Laurer, G. R., T. J. Kneip, R. E. Albert and F. S. Kent. Flourescence: Detection of Lead in Wall Paint," Science, 466-468 (1971). , "X-ray 172: 17. Kneip, T. J., N. Cohen, D. H. Goldstein and E. A. S. Muchmore, "Experimental Studies of Lead Toxicity in Trace Substances in Environmental Health - V," Proceedings of University of Missouri1s 5th Annual Conference on Trace Substances in* Environmental Health (ed. by D. D. Hemphill), pp. 349-361, ( June 19 71) . 18. Eisenbud, M. and T. J. Kneip. "Trace Metals in the Atmosphere," Tech. Paper #16, New York State Department of Environmental Con servation, Environmental Quality Research and Development Unit (July, 1971). 19. Lentsch, J. W., T. J. Kneip, M. E. Wrenn, G. P. Howells and M. Eisenbud. "Stable Manganese and Manganese-54 Distributions in ' The Physical and Eiological Components of the Hudson River Estuary," Proc. Third National Symposium on Radioecology, Oak Ridge (1971). PCB-ARCH-EXT0376790 Curriculum Vitae Page 6 20. Kneip, T. J. "Sampling and Analytical Problems in a Complex Natural System: The Hudson River Estuary," American Laboratory 3_:47-52 (1971). 21. Cohen, N., T. J. Kneip, "The Juvenile Baboon as Poisoning in Children," 142-155 (1972). D. Goldstein and E.A.S. Muchmore, a Model for Studies of Lead Journal of Medical Primatology 1: . 22. Kneip, T. J. and G. R. Laurer. "Isotope Excited X-ray . Fluorescence," Analytical Chemistry, 4:57A-66A (1972). 23. Laurer, G., M. Eisenbud, R. E. Albert, T. J. Kneip and N. 'Nelson. "New York City Health Research Council X-ray Fluorescence Analyzer for Lead," Isotopes and . Radiation Technology 9^: 275-277 (1972) . *24. Kneip, T. J., et al., "Tentative Method of Analysis for Inorganic Lead Content of the Atmosphere," Health Laboratory Science 9 (1) :79-83 (1972) . , 25. Laurer, G. R., R. E. Albert, T. J. Kneip, B. Pasternack, C. Strehlow, N. Nelson and F. S. Kent, "The Distribution of Lead Paint in New York City Tenement Buildings, AJPH 63: 163-168 (1973). ' . 26. Kneip, T. J. and G. J. Lauer. ."Trace Metal Concentration Factors in Aquatic Ecosystems," in Vol. 5, Progress in Analytical Chemistry, Plenum Press, New York^ New York (1973). 27. Ahuja, S., E. M. Cohen, T. J. Kneip, J. L. Lambert, G. Ziweig, eds. Chemical Analysis of the Environment and Other Modern Techniques, Plenum Press, New York, New York (1973)~ 28. *29. Kleinman, M. T., T. J. Kneip and M. Eisenbud, "Meteorological Influences on Airborne Trace Metals and Suspended Particulates," Proceedings, 7th Annual Conference on Trace Substances in Environmental Health, University of Missouri, Columbia, Missouri^ June 12-14 (1973). Kneip, T. J., et al., "Tentative Method of Analysis for Cadmium Content of Atmospheric Particulate Matter," Health Laboratory Science 10_(3) : 214-217 (1973) . *30. Kneip, T. J., et al,, "Tentative Method of Analysis for Molybdenum Content of Atmospheric Particulate Matter by Atomic Absorption Spectrophotometry," Health Laboratory 10(3):218-22Q (1973).* Science *Reprinted in Methods of Air Sampling and Analysis, Second edition, Katz, M., ed. American Public Health Association, Washington, D.C. (1977). PCB-ARCH-EXT0376791 Curriculum Vitae Page 7 *31. Kneip, T. J. , et al., "Tentative Method of Analysis for Nickel Content of Atmospheric Particulate Matter by Atomrc Absorption Spectroscopy," Health Laboratory Science 10 (3) : 2 21 - 2 2 5 (1973). ...... .................. .... *32. Kneip, T. J., et al., "Tentative Method of Analysis for Copper Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 10(3):226-230 (1973). " *33. Kneip ," (T . J. , et al. , "Tentative Method of Analysis for Chromium Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 10 (4) : 358-361 (1973). " .............. ~ =' '....~~. " 34. Kneip, T. J., G. P. Howells, M. E. Wrenn, "Trace Elements, Radionuclides and Pesticide Residues in the Hudson River,"'In: Marine Pollution and Sea Life. Fishing News (Books) Ltd., . Surrey, England, p. 169 (1973). (Reprinted from Ref. 15) 35. Kneip, T. J., M. T. Kleinman and M. Eisenbud, "Relative Contributions of Emission Sources to the Total Airborne Particulates in New York City," Proceedings, Third International Clean Air Congress" October 8-12" 1973 (1974). .36. 37. Cohen, N. ,T. J. Kneip, V. Rulon and D. Goldstein, "Biological and Toxicological Response of Infant Baboons to Lead Driers in Paint," Env. Hlth. Perspectives, Experimental Issue #7, DHEW Pub. # NIH 74-218, U.S. Gov't Printing Office, Washington, D.C., p. 161 (1974). . t Kneip, T. J., N. Cohen and V. Rulon, "Potential Sources of Analytical Error in the Erythrocytic 6-Aminolevulinic Acid Dehydratase Test for Lead Exposure," Analytical Chemistry, 46:1863-1866, (1974). 3* Bernstein, D. M., T. J. Kneip, M. T. Kleinman, R. Riddick and M. Eisenbud, "Uptake and Distribution of Airborne Trace Metals in Man," Proceedings of the 8th Annual Conference of Trace Substances in Environmental Health, University of Missouri, Columbia, Missouri, June (1974). 39. Kneip, T. J., G. Re and T. Hernandez, "Cadmium in an Aquatic Ecosystem: Distribution and Effects," Trace Substances in Environmental Health - VIII, 1974"! A~ Symposium. D. D. Hemphill,' ed. , University of Missouri, Columbia (1974). PCB-ARCH-EXT0376792 Curriculum Vitae Page 8 40. Bondietti, E. A., F. H. Sweeton, T. Tamura, R. M. Perhac, L. D. Nulett, and T. J. Kneijs,. "Characterization of Cadmium and Nickel Contaminated Sediments from,Foundry Cove, New York," In: Proceedings of the First Annual NSF-RANN Trace Contaminants Conference, Oak Ridge, Tennessee, August, 1973. pp. 211-224. CONF-730802, ORNL, Oak Ridge, Tennessee (1974). *41. Kneip, T. J., et al., "Tentative Method of Analysis for Cadmium Content of Atmospheric Particulate Matter by Atomic- Absorption Spectroscopy," Health Laboratory Science 11 (2) : 112-116 (1974). " ......... ' " ' ~ *42. Kneip, T, J., et al., "Tentative Method of Analysis for Iron Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11(2): 118-121 (1974). *43. Kneip, T. J., et al., "Tentative Method of Analysis for Lead Content of Atmospheri-c Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11(2): 122-127 (1974). ~ . *44. ' Kneip, T. J., et al., "Tentative Manganese Content of Atmospheric Atomic Absorption Spectroscopy," 11 (2) :128-133 (1974). Method of Analysis for Particulate Matter by Health Laboratory Science *45. Kneip, T.__J., et al., "Tentative Method of Analysis Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 134-139 (1974). . for Zinc 11(2): * *46. Kneip, T. J., et al., "Tentative Method of Analysis for Vanadium Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11 (3) :240-245 (1974). *47. Kneip, T. J., et al. Tentative Method of Analysis for Elemental Mercury in Ambient Air by Collection on Silver ' Wool and Atomic Absorption Spectroscopy," Health Laboratory Science 11 (4) : 342-346 (1974)' PCB-ARCH-EXT0376793 Curriculum Vitae Page 9 48. Kneip, T. J., M. Eisenbud, M. T. Kleinman and D. M. Bernstein, "Studies of Trace Substances in an Urban Atmosphere," Proceedings of the International Symposium of Recent Advances in the Assessment of the Health Effects of Environmental Pollution, Paris, , June 24-28, 1974. Commission of European "Communities, Luxembourg, p.431-450 . NTIS EUR5360 (1975) . *49. Goldstein, D. H., T. J. Kneip, V. Rulon and N. Cohen, "Erythrocytic 6-Aminolevulinic Acid Dehydratase (6-ALAD) Activity as a Biological Parameter for Determining Exposures to Lead," Journal of Occupational Medicine, 17:157-162 (1975). 50. Kneip, T.'J., "Potential Applications of X-ray Analytical Method in Paint Analysis," Journal of Paint Technology, 47 :71-76 (1975) . .......................... ~ 51. Kneip, T. J., T. Hernandez and Gary Re, "Cadmium in an Aquatic Ecosystem: Transport and Distribution," In: Trace Contaminants in the Environment," Proceedings of the Second Annual NSF-RANN Trace Contaminants Conference, Asilomar, Pacific Grove, California, 29-31 August, 1974 (1975) . 52. Kneip, T. J., et al., "Tentative Method of Analysis for Elemental Mercury in the Working Environment by Collection on Silver Wool and Atomic Absorption Spectroscopy," Health Laboratory Science 12(2):158-162 (1975). 53. Kneip, T. J., et al., "Lead in Blood and Urine," Health Laboratory Science 12 (4): 375-378 (1975). 54. Kneip, T. J., et al., "Mercury in Urine," Health Laboratory Science 12(4) :379-382 (1975). 55. Kneip, T. J., et al., "General Atomic Absorption Procedure for Trace Metals in Airborne Material Collected on Membrane Filters," Health Laboratory Science 1_2(4) :383~393 (1975) . 56. Kneip, T.J., N. Cohen, V. Rulon, David Goldstein and N. Nelson, "Factors Influencing Dose-Response Relationships for Toxic Metals," In: Effects and Dose-Response Relationships of Toxic Metals, G.F. Nordberg, ed., Associated Scientific Publishers, Amsterdam (1976). 57. Eisenbud, M., T. J. Kneip, M. T. Kleinman, D. M. Bernstein, "Trace Metals in Urban Aerosols," Final Report to Electric Power Research Institute, October, 1975. NTIS # Pb-248-324 (1976) . PCB-ARCH-EXT0376794 Curriculum Vitae Page 10 58. Bernstein, D. M., M. T. Kleinman, T. J. Kneip, T. L. Chan and M. Lippmann, "A High-Volume Sampler for the Determination of Particle Size Distributions in Ambient Air," Journal of the American Air Pollution Control. Association 26 (11) , T069-T0 7 2 (1976) . 59- Kleinman, M. T., T. J. Kneip and M. Eisenbud, "Seasonal Patterns of Airborne Particulate Concentrations in New York City," Atmospheric Environment 10_:9-11 (1976) . 60* Kleinman, -M. T., D. M. Bernstein and T. J. Kneip, "An Apparent Effect of the Oil Embargo on Total Suspended Pa-rticulate Matter and .Vanadium of New York City Air," J. Air Poll. Con. Assoc. 27:65-67 (1977). Kneip, T.J., R. Peter Mallon and Virginia P. Rulon, "The Effects of Anemia on Heme Synthesis Parameters During Lead Exposure," American Industrial Hygiene Association Journal 37, 578 -58 5 (1977 ) . 62. Kneip, T. J., et al., "Analytical Method for Antimony in Air and Urine," In: Methods of Air Sampling and Analysis, Second edition, Katz, M., ed. American Public Health Association, Washington, D. C., pp. 765-769 (1977). 63. Kneip', T. J., et al., "Analytical Method for Arsenic in Urine and Air," In: Methods of Air Sampling and Analysis, Second edition, Katz, MTM ed. American Public Health Association, Washington, D. C., pp. 769-773 (1977). 64. . Kneip, T. J., et al., "Analytical Method for Arsenic, Selenium and Antimony in Urine and Air by Hydride Generation and Atomic Absorption Spectroscopy," In: Methods of Air Sampling and Analysis, Second edition, Katz, M., ed. American Public Health Association, Washington, D. C., pp. 774-780 (1977). . 65. . Kneip, T. J., et al., "Analytical Method for Chromic Acid Mist in Air," In: Methods of Air Sampling and Analysis, Second edition, Katz, M., ed. American Public Health Association, Washington, D. C., pp. 785-788 (1977). 66. Kneip, T, J., et al., "Analytical Method for Lead in Air," In: Methods of Air Sampling and Analysis, Second edition, Katz, M., ed. American Public Health Association, Washington, D. C., pp. 808-818 (1977). 67. Hazen, R. E. and T. J. Kneip, "The Distribution of Cadmium in the Sediments of Foundry Cove," In: Hudson River Ecology, Proceeding of a Symposium, Hudson River Environmental Society, ed. March 28-30, 1976 (1977). PCB-ARCH-EXT0376795 Curriculum Vitae Page 11 68. Kneip, T. J., M. T. Kleinman, R. Riddick and D. M. Bernstein, "Trace Elements in Human Tissues," Proceedings of the International Workshop on Biological Specimen Collections, April 18-22 , 1977, Luxembourg, (In press, 1977) . 69, 70. Kneip, T. J., "Effects of Cadmium in an Aquatic Environment," In: Cadmium 77, Edited Proceedings First International Cadmium Conference, San Francisco, 31 Jan.-2 Feb., 1977. Metal Bulletin Limited, London, pp. 120-124, (1978). r '** . Daisey, J. M., I. Hawryluk, T. J. Kneip and F. Mukai, "Mutagenic Activity in Organic Fractions of Airborne Particulate Matter," Presented at the Carbonaceous Particles in the Atmosphere Conference, March 20-24, 1978, Berkeley, California. 71. Kneip, T. J., M. A. Leyko, M. T. Kleinman, M. Lippmann and J. M. Daisey, "Organic Matter in New York City TSP: Comparisons of Seasonal Variations and Relationships to Source Tracers," Presented at the Carbonaceous Particles in the Atmosphere Conference, March 20-24, 1978, Berkeley, California. 72. Lioy, P. J., G. T. Wolff and T. J. Kneip, "Toxic Airborne Elements in the New York Metropolitan Area," J. Air Poll. Con. Assn., 28 (5) : 510-512 (1978). . 73. Leaderer, B. P., D. M. Bernstein, J. M. Daisey, M. T, Kleinman, T. J. Kneip, E. 0. Knutson, M. Lippmann, P. J. Lioy, . K. A. Rahn, D. Sinclair, R. L. Tanner, and G. T. Wolff, "Summary of the New York Summer Aerosol Study (NYSAS)," J. Air Poll. Con. Assn. , 218 (4) :321-327 (1978). 74. Fennikoh, K. B., H. I. Hirshfield and T. J. Kneip, "Cadmium Toxicity in Planktonic Organisms of a Freshwater Web," Environ. Res. June (1978). 75. Kneip, T. J. and M. T. Kleinman, "Sampling and Multielement Analysis of Ambient Aerosols," I_n: Special Environmental Report No. 10, Air Pollution Measurement Techniques, Proceedings, of the WMO Air Pollution Measurement Techniques Conference (APOMET), Gothenburg, 11-15 October, 1976. World Meteorological Organization, Geneva, Switzerland, pp.132-139, (1977). 76. Laurer, G. R. and T. J. Kneip, "Portable X-ray Units for Analyzing Lead in Paint," In: A Report of the Committee on Environmental Improvement: Assessing the Problem of Lead-Based Paint Hazards, pp. 13-17, American Chemical Society, Washington, D.C. (August, 1978) . PCB-ARCH-EXT0376796 Curriculum Vitae Page 12 77. Kneip, T. J. and R. E. Hazen, "Deposit and Mobility of Cadmium in a Marsh-Cove Ecosystem and the Relation to Cadmium Concentration in Biota." Environ. Health Persp. Feb. (1979). 78. Lowenberg, R. and T. J. Kneip, "Dietary intakes of lead and cadmium in vegetables grown in New York City." Environ. Health Persp. (In press, 1979). 79. Kneip, T. J. "Concentrations of lead and cadmium in . garden vegetables grown in New York City. ". Environ. Health Persp. (In press, 1979). r '1> t , 80. Hazen, R. E. and T. J. Kneip. "Biogeochemical cycling of cadmium in Marsh Ecosystem." In: Biogeochemistry of Cadmium, Wiley and Sons, New York. J. 0. Nriagu, Ed. (In press, 1979). 81. Daisey, J. M., M. A. Leyko and T. J. Kneip,- "Source identification and allocation of PAH compounds in the New York City aerosol: Methods and applications." In: Polynuclear Aromatic Hydrocarbons, P. W. Jones and P. Lever, eds. Ann Arbor Science Publishers, Inc. Ann Arbor, Michigan (In press, 1979) pp. 201-215.. 82. Kneip, T. J., M. Lippmann, F. Mukai and Trace Organic Compounds in the New York Part I. Preliminary Studies. Report to Research Institute. (Ia press, 1979). J. M. Daisey, City Atmosphere. Electric Power 83. Kneip, T. J. and M. Lippmann, Eds. New York Summer Aerosol Study. Annals of the New York Academy of Sciences. (En press, 1979). 84. -- Kneip, T. J. and P. J. Lioy, Eds. Aerosols: Anthropogenic and Natural--Sources and Transport. Annals of the New York Academy of Sciences. (In press, 1979). 85. Kneip, T. J., Reviews on 1) Haloethers and Haloalkylethers; 2) Phthlalate Esters; 3) Nitrosamines; 4) Dichloro- benz'idine. In: Chemical Pollutants of New York Bight: Priorities, J. S. O'Conner and H. M. Stanford, eds. ' NOAA/MESA Report (In press, 1979).' PCB-ARCH-EXT0376797 CURRICULUM VITAE JOSEPH MARTIN O'CONNOR PERSONAL EDUCATION r -* . HONORS AWARDS SOCIETIES AFFLILIATIONS Born: November 19, 1944, Boston, Massachusetts Married: 2 children . Bachelor of Science, Biology June, 1966 College of the Holy Cross Worcester, Massachusetts (Philosophy minor) Doctor of Philosophy, Ecology/Physiology November, 1971 State University of New York at Albany Albany, New York Dissertation Title: Photoperiodic control of pituitary gonadotropin release in trout (Salmo gairdneri Richardson and Salvelinus fontinalis (Mitchell) and in the . leopard frog (Rana pipiens Shreber) Sigma Xi Beta Beta Beta Biological Society Student representative to Faculty Senate State University of New York at Albany R.E.S.A. (Sigma Xi) Research Grant-in-Aid N.S.F. Pre-doctoral fellowship (Honorable) Research Grant-in-Aid, Edmund Niles Hyuck Preserve Fellowship, Research Foundation of New York State New York Academy of Sciences . American Society of Limnology and Oceanography Estuarine Research Federation Atlantic Estuarine Research Society AAAS . American Society of Natural History Ecological Society of America. American Fisheries Society . Sigma Xi Great Lakes Research Society Florida Defenders of the Environment Board' of Consultants, Lawler, Matusky and Skelly Engineers, Tappan, New York PCB-ARCH-EXT0376798 2 EXPERIENCE present 1974-75. 1973-74 1970-73 1967-70 1965-67 (summers) PROFESSIONAL ACTIVITIES 1 Assistant Director Laboratory for Environmental Studies Institute of Environmental Medicine New York University Sterling Forest, Tuxedo, New York Senior Biologist ' Office of Professional'and Technological Development Lawler, Matusky and Skelly Engineers 415 Rt. 303, Tappan, New York Director Biological Studies Section and Laboratory Office of Research and Technology Quirk, Lawler and Matusky Engineers and ' QLM Laboratories, Inc. Tappan, New York Research Associate . University of Maryland Natural Resources Institute Hallowing Point Field Station Prince Frederick, Maryland Graduate Research Fellow Dept, of Biological Sciences State University of New York at Albany Faculty Research Assistant University of Maryland " Natural Resources Institute Chesapeake, Biological Laboratory ' Solomons, Maryland . Member, Advisory Committee . Chesapeake Bay R.A.N.N. Program of the National Science Foundation . K 1971-1972 U.S. Dept, of Agriculture Graduate School Programs in Aquatic Ecology and Pollution Biology Counselor Rockland County Guidance Center for Women Guidance in Career Programs Adjunct Assistant Professor Inst, of Mar. and Atmos. Sciences of the City University of New York PCB-ARCH-EXT0376799 Advisor, Fisheries Biology U.S. Dept, of Commerce National Oceanic and Atmospheric Administration New England Offshore Mining Environmental Study 1972-1974 ' - Visiting Investigator University of Delaware College of Marine Studies Bayside Laboratory Lewes, Delaware . 1972 RESEARCH INTERESTS Population dynamics Community dynamics - . Respiratory physiology of fishes Reproductive physiology of fishes Chemical-biological interrelationships in Ethology of fishes ecology TEACHING Physiological ecology . INTERESTS General physiology . General ecology Pollutio'n biology Ichthyology \ PUBLICATIONS O'Connor, J.M. 1969. The effects of melatonin on in vitro ovulation of frog ovarian oocytes. Amer. Zool. 9:549. O'Connor, J.M. and R.G. Stross. 1970. Photoperiodic control of the pituitary gonadotropin release patter of the frog, Rana pipiens Shreber. Abstr. Proc. N.E. Division Comp. Physiology section, Amer. Soc. Zool. ' 1 O'Connor, J.M. 1970. The effects of thermal shock on the tidal activity patterns of the hogchoker, Trinectes maculatus. Abstr. Amer. Society of Ichthyologists and Herpetologists, Ann. Mtg., New Orleans, La. O'Connor, J.M. 1971. Photoperiodic control of the pituitary gonadotropin release patterns in the rainbow trout (Salmo gairdneri (Rich.,)), the brook trout (Salvelinus fontinalis Mitchell) and in the leopard frog (Rana pipiens Shreber). Ph.D. Thesis. Library, State University of New York at Albany, and University Microfilms, Ann Arbor, Mich. PCB-ARCH-EXT0376800 4 O'Connor, J.M. 1972. Pituitary gonadotropin release patterns in rainbow trout (Salmo gairdneri) brook trout (Salvelinus fontinalis) and in the leopard frog (Rana pipiens). Comp. Biochem. Physiol. 43(A):739-746. O'Connor, J.M. 1972. Tidal rhythm in the activity patterns of the hogchoker, Trinectes maculatus (Bloch and Schneider). Exp. Mar. Biol. Ecol. 9:173-178. Sherk, J.A., J.M. O'Connor and D.A. Neumann. 1972. The effects of suspended and deposited sediments on estuarine organisms. Univ. Md. N.R.I. ref. 72-9. 1 Sherk, J.A., J.M. O'Connor and D.A. Neumann. 1973. The effects of suspended and deposited sediments .on estuarine organisms. Univ. Md. N.R.I. ref. no. 73-4. O'Connor, J.M., R.W. Williams and J. Converse. 1974. Studies of fish impingement at the Nine Mile Point Nuclear Station, Lake Ontario. Abstr. 17th Conference on Great Lakes Re search. Int'l Assn, for Great Lakes Research. O'Connor,- J.M. and J.A. Sherk. 1974. The responses of some estuarine organisms to Concentrations of suspended solids.' Proc. of the Seventh Dredging Seminar, Ctr. for Dredging Studies, Texas A&M University. Sherk, J.A., J.M. O'Connor and D. Neumann. 1975. The effects of suspended sediments on estuarine environments. IN: L.E. Cronin (Ed.). Estuarine Research, Vol. II. Geology and Engineering, Academic Press, N.Y. pp. 541-557. Neumann, D.A., J.M. O'Connor and J.A. Sherk. 1975. Respiratory and hematological responses of the oyster toadfish (Opsanus tau) to suspended sediments. Trans. Amer. Fish. Soc. ToT: 775-781. Sherk, J.A. and J.M. O'Connor. 1976. Effects of sedimentation ' on coastal zone organisms. Proc. Ill Federal Inter-Agency Sedimentation Conference. O'Connor, J.M. and S. Schaffer. 1977. gear on striped bass ichthyoplankton. 18:312-315. Effects of sampling Chesapeake Science Sherk, J.A., J.M. O'Connor and D.A. Neumann. 1976. of suspended solids on selected estuarine plankton. Army Corps of Engineers Coastal Engineering Research Misc. Rept. 76-1. 50 pp. Jan. 1976. Effects U.S. Center. PCB-ARCH-EXT0376801 O'Connor, J.M., D.A. Neumann, and J.A. Sherk, Jr. 1976.' Lethal effects of suspended sediments on estuarine fish. Technical Paper No. 76-20, U.S. Army, Coastal Engineering Research Center, 38 pp. O'Connor, J.M., D.A. Neumann, and J.A. Sherk. 1977. Sublethal effects of suspended sediments on estuarine fish. Technical Paper No. 77-3, U.S. Army, Coastal Engineering Research Center, 90 pp. Rachlin, J. , A.P. Beck, and J.M. O'Connor. 1978 . Karyotypic analysis of Hudson River striped bass, Morone saxatilis (Walbaum). Copeia 1978(2):334-336. Ginn, T.C*. and J.M. O'Connor. 1978 . Responses of the estu arine amphipod Gammarus daiberi to chlorinated power plant effluent. Estuarine Coastal Mar. Sci. 6:459-469. Poje, G.V., T.C. Ginn, and J.M. O'Connor. 1978. An opera tional system for simulating the physical and chemical en vironment of the condenser tubes of a power plant. IN: Thorp, J. and J.W. Gibbons, eds. Energy and Environmental Stress in Aquatic Systems (in press). Zubarik, L.S., and J.M. O'Connor. 1978. A radioisotopic study of mercury uptake by Hudson River biota. IN: Thorp, J. and J.W. Gibbons, eds. Energy and Environmental Stress in Aquatic Systems (in press). O'Connor, J.M., C.C. Lee, and M. Eisenbud. Environmental Health. M.E.S.A. New York Bight Atlas Monograph 32 (in press) . . Ginn, T.C., G.V. Poje, and J.M. O'Connor. 1978. Survival of planktonic organisms following passage through a simu lated power plant condenser tube. Proc. Fourth Nat'l Workshop on Entrainment and Impingement. pp. 91-101. PUBLICATIONS IN REVIEW O'Connor, J.M., D.A. Neumann and J.A. Sherk. The responses of estuarine fishes to highly turbid environments. Water Res. O'Connor, J.M..and J.A. Sherk. The effects of suspended mineral solids on the white perch, Morone americana. Trans. Amer. Fish. Soc. O'Connor, J.M. and J.A. Mihursky. The effects of thermal shock on the activity patterns of the hogchoker, Trinectes maculatus. Ches. Sci. 6 MANUSCRIPTS IN PREPARATION ' ' Eichorn, K.L., T.C. Ginn and J.M. O'Connor. Response of striped bass (Morone saxatilis) to chlorinated cooling water. O'Connor, J.M., G.V. Poje, and S. Rigdon. Respiration of striped bass (Morone saxatilis) eggs and larvae. Becker, A.J. and J.M. O'Connor. Aerobic shutdown in the green crab (Carcinus maenas) in response to temperature, salinity and sulfide. - Steinhausler, F., S. Schaffer, N. Cohen, C. Lee, J, O'Connor and.M. Wrenn. Effect of high LET radiation on intracellular ATP Content of prokaryotic and eukaryotic algae. Hendrick, E., J.M. O'Connor, and G.R. Laurer. A method for analysis of trace mineral content of fish otoliths using X-ray fluorescence spectroscopy. . . PRESENTATIONS ' '* * O'Connor, J.M. 1969. The effects of melatonin on in vitro ovulation of ovarian oocytes in the frog, Rana pipiens. Amer. Soc. Zool. O'Connor, J.M. and R.G. Stross. 1969. Photoperiodic control of the pattern of pituitary gonadotropin release in the frog Rana pipiens Shreber. Amer. Soc. Zool. O'Connor, J.M. 1966. Alterations in the tidal activity rhythm in the hogchoker after exposure to thermal shock. Atlantic Estuarine Research Society, Cambridge, Md. October, 1966. * O'Connor, J.M. and J.A. Sherk. 1971. The lethal effects of suspended solids on estuarine fishes. Joint Mtg. Atlantic Estuarine Research Society and the Estuarine Research Federation. Pleasantville, N.Y. October, 1971. Neumann, D.A., J.M. O'Connor and J. Sherk. 1972. Studies of the respiration of the striped bass and the white perch exposed to suspended sediments. Atlantic Estuarine Research Soc. Ocean City, Md. April, 1972. O'Connor, J.M., J.A. Sherk and D. Neumann. 1973. "Inert" - mineral compounds as potential factors in mortality and toxin transport in estuarine organisms. E.P.A. Workshop on Chemical Pathogens in the Environment. Pensacola, Fla., May, 1973. PCB-ARCH-EXT0376803 7 Sherk, J.A., J.M. O'Connor and D. Neumann. 1973. The effects of suspended solids on estuarine environments. Symp. on Recent Advances in Estuarine Research. II Int'l. Conference of the Estuarine Research Federation. Myrtle Beach, S.C. October, 1973. O'Connor, J.M., and J.A. Sherk. 1974. The responses of some estuarine organisms to concentrations of suspended solids. Seventh Dredging Seminar. Sp. by the Center for Dredging Studies, Texas A&M University, New Orleans, La. November, 1974. O'Connor, J.M., R. Williams and J. Converse. 1974. Impinge ment studies at the nine mile point nuclear power station, Lake Ontario. 17th Conference on Great Lakes Research, . August, 1974. Hamilton, Ontario, Canada. O'Connor, J.M., and S.G. O'Connor. 1975. Seasonal Compartmentalization of primary productivity in the New York Bight, 1973-74. Am. Soc. of Limnology and Oceanography, Halifax, N.S., Canada, June, 1975. O'Connor, J.M. 1976. Assessment of entrainment impact at the Indian Point Nuclear Station. Third National Work shop on Entrainment and Impingement, New York City. Poje, G.V. and J.M. O'Connor. 1976. Gammarus tigrinus: an estuarine predatory carnivore. Presented to the Atlantic Estuarine Research Society. May 1976. Ginn, T.C., K.L. Eichorn and J.M. O'Connor. 1976. Response of the striped bass Morone saxatilis and the amphipod Gammarus to chlorinated power plant effluent. Northeast Fish and Wild life Conference. April 26-29, Hershey, Pennsylvania. Poje, G.V., T.C. Ginn and J.M. O'Connor. 1977. Use of a condenser tube simulator for evaluating power plant entrain ment effects. Northeast Fish and Wildlife Conference, Boston, Massachusetts. April 3-6, 1977. Poje, G.V., T.C. Ginn, and J.M. O'Connor. 1977. An operational system for simulating the physical and chemical environment of the condenser tubes of a power plant. Symposium on Energy and Environmental Stress in Aquatic Systems, Augusta, Georgia, November, 1977. Zubarik, L.S., and J.M. O'Connor. 1977. A radioisotopic study of mercury uptake by Hudson River biota. Symposium on Energy and Environmental Stress in Aquatic Systems, Augusta, Georgia, November, 1977. . PCB-ARCH-EXT0376804 8 O'Connor, J.M.,C.C. Coutant, and J.P. Lawler. 1977. Environ mental impact assessment as ecological research. Symposium on Energy and Environmental Stress in Aquatic Systems, Augusta, Georgia, November, 1977. O'Connor, J.M., T.C. Ginn, and G.V. Poje. 1978. Survival of planktonic organisms following passage through a simulated power plant condenser tube. Fourth Nat'l Workshop on En trainment and Impingement. Chicago, Illinois. Steinhausler, F., S. Schaffer, N. Cohen, C. Lee, J. O'Connor, and M. Wrenn. 1978. Effect of high LET radiation on in.tra- . cellular ATP content of prokaryotic and eukaryotic algae. Radiation Research Society. Toronto., Ontario, Canada. PCB-ARCH-EXT0376805