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AR22-6 3295 . ant ARR26 _ 3295 Dupont 13216 -- TRADE SECRET Study Title H-25878: 150 Biocompatibility Testing STUDY MONITOR: Carol Finlay, B.A. STUDY COMPLETED ON: August 26, 2003 PERFORMING LABORATORY: North American Science Associates, Inc. 2261 Tracy Road. Northwood, Ohio 43619-1397 -- for HEaLskdeullPLoanbtodreatNoermyofuorrsHeaanldthCaonmdpEannvyironmental Sciences Elkton Road, P.0. Box 50 Newark, Delaware 19714-0050 ones (0) LABORATORYPROJECT ID: DuPont-13216 `Company Sanitized. Doss not contain TSCA CBI Petom 125878: 150 Biocompatily Tesing Dupont 13216 --_ TABLE OF CONTENTS Page STUDY INFORMATION wormed AAPPEH2N5D8I78CECYSTo OTOXIs CITY s STUDY USING THE 150 AGAROSE OVERLAY METHOD B. H.25878 150 INTRACUTANEOUS STUDY. " C._ H2S878USP MUSCLE IMPLANTATION STUDY. vrscorner nal? mmm --_ Pp -_--m-- CompanySaniized. Dossfot containTSCACB A ~ stance Toe: EES STUDY INFORMATION Synonyms/Codes: + H-25878 Haskell Number: 25878 -- `KnownImpurities: None Sponsor: E.L du Pont de Nemours and Company Wilmington, Delaware 19898 USA. ~~ Study Initiated/Completed: June 26, 2003 /(see report cover page) pySaitend onsen SNS H25878: 150Biosompaibity Testing Dupont 13216 SUMMARY AretstualctshefdorarteherHep-o2r5ts87(8ApspaemnpdlieceasreA: - C) from North American Science Associates, Inc. The Test Results Cytotoxicity ISO Intracutaneous 1SO Muscle --d HE eo a Ne Test Substance (Agarose Overlay) Sct CSO' Implantation(7 day) H-25878 e d d NS a CSootdiounmscahdloroiildeexetrxactc 4 PNNrooiecmviaidrdyeenanccteeioafofsnieglInnifbdieicxsanCothraitmroicxaiitcroiztnya.tons Negligible Notsignificant Study Monitor: --_ Carol Fray B.A[. StuffToxicologist 26- DHateug 243 `Company Sanitized. Does not contain TSCA Cal TTT Tr H-25878: 150BiocompatibilyTesting DuPont13216 APPENDICES ~~ Senlizod, Doo nog contain Tscp gy - B2H-528578788::119500BBliooccoonmppaattbbiilltiyyTTeessttiinngg -- DDuuPPonotm1l32s166 -- APPENDIX A H-25878 Cytotoxicity Study Using the ISO Agarose Overlay Method `Company Sanitized. Doss not contain TSCA CBI _-- pr LS 23W 878:o 150 BiB sompaio bily Teso ting mpuyTei oDwoemwy 1sz2n1e6 ~~ . cots wore oc: CrToroNITYspy usso r80yAROSE OVERLAY THD coun mawamsan s - wmfaHeiEnntaasn: Peli Scf asrRoo nmSai ny, sonurony TE eae fo IER --- Company Saited. Doss not contain TSCA CBI -_-------- 2587: 150 BiscomptbityTein C-- pr fr-- we RR) Baie --_ NAMSA In Emme -- -------------------- --- GomponySaitzed.Dossotcontain TSGACE - 00000000 B2a88s78: o150bBioscoTTeaiing oDwunpse - . i --] assiz we [i] (me kt tf ettpt tirer Aoinvitrobiocorspatibiltystudy,bsedontherequirementsofthe leteroationalOrganizationforStasdardizatcn E es adie nRSTon piAn fA r2aitie,s R I lr any en et tin-- y,T--e - Pa Sere E E fmA,E `LiooelD.Brock,BS > or Ed Sand MarthaGswanski, 65] LAT Foe Uy 3, 2003 DuseCompleted. re --~ - compen Sait.Dossot onanTSCACE! 00000+ poowentess es [m C2 n sme m pe we (o--) ia Ee HEo RrEte Snr : Hong Be do Boe rer tttose tsh tw--e Swor rr ---- eee -- ~ ree -- ( -- | rS E EenRmnEeRLPEADsnE u cy n fS of9croen 003n Tf oon BS em sT aRseeEshE mpiT R n fo x rr sree 125878:150 Biocompatbily Testing Dupont13216 - oI --] Lv, LDomuasns') esl eDT Spiyerreir nd t.v BehFlooeiolS tttwehta eeOSrEA p ecsteit 0SeSisa,eSe sAyTCare Folosldliicdgoenpoan,ynl(8sf)cwe mind ry croscpcty tac es orcldheen BSe B ToRndhs esiwiyld Suingfecncmsds8 loving i: FCoo m T[hdeeem T|ommasi csommdmooommsg | 2h on einaon essecm | Tove meresponimmo ies--| T oyeen Gn seE coed ---- Foe rreoli iBtSymfehyeneyrdkt sFptobdettcoooeatmEbeP let pShep 2e peus 0aet vshi8dee e0Gidcyiteyso)e --~ recaohe si lsl Th rshwe dom: sass T Er waw s w o v [ous s |mei] @o .. .. Team Got w@ voev Foecot o . . @0 me nasser J ~ Company SSaannliltized. Doss notcontai.n Ts cay - ~ : (sI Erumamn ) ES e GDuPoctR 13216, E EE m tR e r cnc e ter mtsnvJ otei NAMSA --- wep ~~ BompenySavitzeq poo, otcontain 7504 oy HL25878: 150 Biocompatibilty Testing -- DuPont 13216 -- APPENDIX B H-25878 ISO Intracutaneous Study ~~ -_-- CompanySanitzed.Dass not contain TA Gay oaesNwommatioTeiwswee - c=s] -_-- = roana--tmaaessnt -- ws E-- owyeo mH EsSosEaenR r U pr EEE en noes -nnncomn pany S- n bosote so- n Ts SOACH EF saan - -- uCCi ~ esas ~ S=oo `Sanitizoq.pq - en aca 3 = LO 2a50w78b 150Bco ompaiw yTsngpubioTe p-- ues s --_ soncnz rn Tn T RS i agln U i Etro de Te S E S La t sBm ilet om l e e eTte i yr E: e re ire ts JS E EE mn ee re nydmtspmeter pres E = i EEuEaEREr --~ ir ad (EET Bris D. Moslager, By dm Completed NsAaMSA --. E RAERE erRs I SYSerine,Does, "cry. H5878: 150 Biocompatbily Testing Dupont13216 ng) ne (Cgcn) Diectttsuiotl:eeTtntefnblinOaspsiinniSeodendSs`e.nimcToocopuspnevaresos5o9f:e ePidfoe B rmh a n bed ne ITce ooht ko vcedc Eaayd .oeen2c .o 300.To o istwetboose Mon mi. he alpovided Tesi ito: Sorgen ver: arms posea td nd td ms nas Netspiod Ros tempers Sar stm c0t SPwisn50 [I --~ Continof int s w1a ep =P=e Teese: Emons HSsospmei = Noto5mkvriatevliosn camer) A Maiweee neriot fboSolepruvsiion eomieionstat pe nfTo Se Decti s coomnetre ntnis. eidhc150 in ames hs ei raters Sete Benes Ach - ~ 0 epre s =tJeAr TEr on. CRY wesserssacons rer mE ma c e vstr o--r -- -- HS mee EsEE as "E BE E SFFhEn f E E ee R rarE e mE enses ~ I rt Pm o Em TE--r ------------ EE S E B e E E E E s E sne S r Tt E Et RT S s NAMSA ===mpmmimssses wn ~~ Company SanDosisnoitcozntaienTSdCAG.BI ES 150Bonny Teng Duroni1g fd CI SoIte rTR ommist eescne34.e4en7sry,rteespirt E Ea WEermEebRa vym TeEEonAGR)eri mune|||[|1[ |[E[ e V [[EeNw eeBeee E sma o!mn uE rn ont eS ne| ER E E EE LLtore he ha iE eednso cB eneRerh L, ect EScRet TE lt S Bm ectl ot nstnrrTt JtbrE.op nnpg on EethEso. --_ i Appmmtmetmtrsonetyy. iisyt hyAtane rors CEE EEE]=] S[(rC TPBBeTsee ete reer t aea a aT d= e ow | | we] ee m NAnMSA pers E aE Raa: -- --- "PanySani,29 etc% ont, "scacay EHE2597:150 BiocompatTheisitnyg Dupo 13216 3 a --_ fale einTh-- ey coiscuuC sion e Aen pigs cy nd cp AscoRDsTOMGE i rdrp oeed sp AMSA ve phate -- -Y Prone PT Com Sent _ 00not omy "ihTSA ogy s anno:no0B T me temsiytas orws emn - fom) sms re em---- es E eh n e : : HHHE ~ a sEaomm SA Fa 20 nay, Wentc ns nsP98 80C8 rrr PR cy - CompenySontized.Doggpy,Contain Tsca ogy =o 1258m78:o 150 Bic ocompaibo iliy Tesm ting ubliyei wDupeons tai32n16 -- Pa. APPENDIX C H-25878 USP Muscle Implantation Study --~ -Y COmDany Sanitizeq,Do toni 154 cap ds osoveemeotepssse ~ : -- me pony Do-Poor-1321 mraesoy oo -- ~ EBorrizaan C2 nrrarnrorm BEE en era ~ terres esserebee CompanySanitized. Does not conta' TSCACBI - } =) sss one RR= SV ---- rS oe E ~ ~~ -_-- -- Company Sanitized. Does notcontain TSCA CBI HIST:150 Bocompatbiy Testing Don13216 - Gong ov Gl -- i anna eT na-- Tet AT re ---- fe -- implantmages. The implantedtes arclemet heUSPrequirements. ee otwitty B m S ork e Bh B ag R47ALT NES Airity. CE iD.2Es 5s -- DLatheCfomrpleetedn Psi --~ - ee nosery TE Company Saniizad. Donotecontsain 75cA cat ~I mT ed) ss s eron l"y EEon EEme TEkEelveSE we ---- or ams---- C".e we ------ ~ -- ( ees | . gees fBEeryowWen EHRSireen3eesmtss Air tEEmeiamonEpmser ta:e EOEMita Seo atas t iEEes sen tya e toey me esema------ ose Breysmas sms rn LL 525878 150 Bicompaiiy Testing Duwonisig (om) So Piry lm on piG)i OF ee ete neem Soc Commins RRaouilcecserns we el th ps Ho Ee ocueiogvhe smelr,yseb meni t ceoteacdog gy cd hers ol. petseee rst Toe by vom rage of 61-72F. i.iTb htmending some geng a, na. EE `eWelhetsp Nessoceoe n 1cwith RtetolindeofHedalt, fetomei Pet Sr, A Atsyos deteeptToyo ylbtrbeteitm,oc ttdrfeii.,rae e ae n A t etTT -- E p Fts h a --B evI et t pv s sc.e u, spimettssaonot m erm lonme atapdbfFee gbeedo e ct g of R e ee rmm R Ee t mn toe he ele eyTele mo= LB s cre e ly eet.St pea nt te NAMSA ~ EE -------------- spony Sd Company Sani od.DosnotcomTotcaonpp ee H258: 150 DiscompuiTbsitngy Dron. 13216 - Ls--P Ak]TwftebT own io of e on. EDuE-tPiocnRte-13pE2e)t. SR aS em oe, Hs j 5ia s dr tsetmnt Limmms r iEntepocneioce iisrLntr eemmp ideeevrcsCttCTh0 Stt,, mEEpeaeT memrtei teSe L 0enrho Co to: Aco ped ness lyrd eheo,Bywig F=O = J ype Dir, dasaforindividualrabbitswereconsideredacceptable, oi Body -- a So i nn ecea mted dtree etopeety EUrEb Rtons fedscncossaus may pci opsees l secomsronaas : awdnapeinfo i dysd 3 copyofthe fil pertarfobretained esgesedNAMAachive : near = PE -- - COmPeny S220. Does rt comin Tc03 o mmo cca 1 125878: 150 Biocompatily Testing Durent13216 rasmeT = Tw= : [=3 Le. =r=osmg amp BODYVETSANDNACHOSOPCORSERVATIONS ------ To 5 [=| T= : : rons ery nis by Fae crs, ~~ NfAeMeSA smeT sgrmie msse: Prot -- 5 CompanySanitizgqD,ocs not conta 7cmy e-- o ------------------