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Surface Protection Solutions 4417 Lancaster Pike, BMP23-2236 Wilmington, DE 19805
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May 30, 2008
Attn: Jim Willis (phone 202-564-0104)
Document Control Office (7407M) United States Environmental Protection Agency (EPA) Office o f Pollution Prevention and Toxics (OPPT) EPA East, Room 6248 1200 Pennsylvania Avenue, N. W. Washington, D. C. 20460
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Dear Jim,
As part o f DuPont's voluntary sharing o f information on our biodegradation research with EPA, enclosed is a Company Sanitized copy o f the final study report for a two-year aerobic soil biodegradation research study conducted by Wildlife International (Project No. 112E-108) for DuPont. We would like to meet with you and discuss the study and our interpretation o f the results. We will follow up in the near future to schedule time.
We plan to publish this work and anticipate submitting a manuscript this summer. Thank you for offering to wait to post this to the Docket until publication. We don't think this will be necessary, however we can discuss it further when we meet.
Please contact me if you have any questions regarding the study.
63100000010/S
Robert C. Buck Tel. 302 892 8935; Email robert.c.buck@usa.dupont.com
Company Sanitized
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Company Sanitized. Does not contain TSCA CBI.
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[ ]: TRANSFORMATION POTENTIAL IN AEROBIC AND ANAEROBIC SOILS
WILDLIFE INTERNATIONAL, LTD. PROJECT NO. : 112E-108
Organisation for Economic Cooperation and Development OECD Guideline 307
AUTHORS: Edward C. Schaefer
Joel Stenzel Raymond L. Van Hoven
STUDY INITIATION DATE: March 10, 2005 STUDY COMPLETION DATE: March 17, 2008
Submitted to: E.I. du Pont de Nemours and Company
Wilmington, Delaware 19898 USA .
Wildlife International, Ltd.
8598 Commerce Drive Easton, Maryland 21601 USA
1 (410) 822-8600
Page 1 o f 298
Com pany Sanitized
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Wildlife International, Ltd,
ProjectNo.; 1I2E-208
2- GOOD LABORATORY PRACTICE COMPLIANCE STATEMENT
SPONSOR: E.I. du Pont de Nemours and Company
TITLE:
^ Transformation Potential in Aerobic and Anaerobic Soils
WILDLIFE INTERNATIONAL, LTD. PROJECT NO.: I12E-108
STUDY COMPLETION DATE: March 17, 2008
This study was conducted in compliance with Good Laboratory Practice Standards as published by the U.S. Environmental Protection Agency in 40 CFR Part 792 and OECD Principles of Good Laboratory Practice (ENV/MC/CHEM (98) 17), with the following exceptions:
Periodic analyses of well water for potential contaminants were performed using a certified laboratory' and standard U.S. Environmental Protection Agency analytical methods.
The characterization of the test substance, and the stability of the test substance under the conditions of storage at the test site, were not determined in accordance with Good Laboratory Practice Standards.
Original documentation for the preparation of two combined stocks were not maintained. Documentation of the stocks preparation was re-created from the information on die stock label.
STUDY DIRECTOR:
Edward C. Schaeid Director of Biodegradation
17 rn/U-ctt U #? DATE
b9stn8 ynCfmoO
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Wildlife International, Ltd.
Project No.: 112E-108
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QUALITY ASSURANCE STATEMENT
This study was examined for compliance with Good Laboratory Practice Standards as published by the U.S. Environmental Protection Agency in 40 CFR Part 792 and OECD Principles of Good Laboratory Practice (ENV/MC/CHEM (98) 17). The dates of all inspections and audits and the dates that any findings were reported to the Study Director and Laboratory Management were as follows:
ACTIVITY: Protocol Stock Solution Preparation
DATE CONDUCTED: March 1,2005 March 15,2005
DATE REPORTED TO:
STUDY DIRECTOR:
MANAGEMENT:
March 1,2005
March 11,2005
March 15,2005
March 16,2005
Headspace Sampling
March 24,2005
March 24,2005
April 13, 2005
Stock Solution Preparation
April 19, 2005
April 19,2005
April 22, 2005
Analytical Data Day 0 Analyses
October 10-26,2005
October 26,2005
November 8, 2005
Analytical Data Week 1 Analyses
October 10-28, 2005
October 28,2005
November 21,2005
Analytical Data - Week 2 Analyses
October 10Novembcr 1,2005
November 1,2005
November 21,2005
Analytical Data - Month 1 Analyses
October 0November 1,2005
November 1,2005
November 21,2005
Analytical Data - Month 2 Analyses
October 10November 1,2005
November 1, 2005
November 21, 2005
Analytical Data - Month 4 Analyses
October 10November 2, 2005
November 2, 2005
December 1,2005
Soil Moisture Determination and Dosing Data
October 10December 2,2005
December 2,2005
August 30, 2006
Oxygen Measurement
October 17, 2005
October 17, 2005
October 19, 2005
Dosing Viability Controls
October 17,2005
October 17,2005
September 26, 2006
Microbial Biomass Sample
Processing
.
October 18, 2005
October 18,2005
November 3, 2006
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Wildlife International, Ltd,
Project No.: 112E-108
ACTIVITY:
Analytical Sample Processing (6-month)
Month 6 Analytical Data
Analytical Draft Report
9 and 12 Month Analytical Data and Tables
Biological Data and Draft Report
Data and Draft Report (including 15,18 and 24 month analytical data)
Final Report
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QUALITY ASSURANCE STATEMENT (continued)
DATE CONDUCTED:
DATE REPORTED TO:
STUDY DIRECTOR:
MANAGEMENT:
October 20,2005
November 14-17,2005
November 17-22, 2005
August 21-25 and 28, 2006
October 23 November 9, 2006
October 24 November 2, 2007
October 20, 2005 November 17, 2005 November 22, 2005
August 29, 2006 November 9, 2006
November 2,2007
October 24, 2005 November 28, 2005 December 1, 2005
October 3, 2006 December 18, 2006
November 20,2007
March 17,2008
March 17,2008
March 17, 2008
All inspections were study-based unless otherwise noted.
Linda R. Mitchell Director of Regulatory and Ecotox Operations
/ 7 M/huJ 0C& DATE
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Wildlife International, Ltd.
Project No.: 112E-108
-5 REPORT APPROVAL
SPONSOR: E.I. du Pont de Nemours and Company TITLE: J Transformation Potential in Aerobic and Anaerobic Soils WILDLIFE INTERNATIONAL, LTD. PROJECT NO. : 112E-108
STUDY DIRECTOR:
Edward C. Schagrer Director of Biodegradation
/7 DATE
Q-eoj
MANAGEMENT:
lUn-rs D , \^ros?e/
Heiuy O. Krueger, Ph.D.
'
Director of Aquatic Toxicology/Terrestrial Plants and Insects
3
DATE
Willard B. Nixon, Ph? Director of Chemistry
DATE
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
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TABLE OF CONTENTS
Title Page.........................................................................
Good Laboratory Practice Compliance Statement......
Quality Assurance Statement........................................
Report Approval..............................................................
Table of Contents...........................................................
Study Information...........................................................
Introduction.....................................................................
O b je c tiv e ......................................................................... ?
Experimental D esign......................................................
Materials and M ethods................................................... Test Substance.......................................................... Measured Analytes................................................... Internal Standards..................................................... Test Soils................................................................... Test Apparatus and Conditions............................... Soil Pre-Incubation................................................... Preparation o f the Test Chambers.......................... Maintenance of Test Chambers............................... Sampling and M easurements.................................. Quality Criteria......................................................... . Viability Assessments............................................. .
Results............................................................................. . Test Conditions......................................................... Characterization of Soils.......................................... Sample Processing and Analyses............................ Headspace Analyses.......................................... Soil Analyses...................................................... Stock and Calibration Standard Preparation.... Calibration Curves and Limits of Quantitation.
References
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...2
...3
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17
17
17
18 18 18 21 22 23 23 23 24 25 25 26
26 26 27 27 27 27 29 31
34
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TABLE OF CONTENTS
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TABLES
Table 1. Frequency of Measurements..............................................................................................................35 Table 2. Test Chamber Headspace Oxygen Content..................................................................................... 36 Table 3. Selected Soil Properties..................................................................................................................... 37 Table 4. Soil Viability by Glucose Mineralization:Alfisol...........................................................................38 Table 5. Soil Viability by Glucose Mineralization :Inceptisol..................................................................... 38 Table 6. Soil Viability by Glucose Mineralization:Ultisol...........................................................................39 Table 7. Soil Viability by Glucose Mineralization:Mollisol....................................................................... 39 Table 8. Soil Microbial Biom ass..................................................................................................................... 40 Table 9. Typical GC/MS Operational Parameters for Analysis of 8-2 FTOH in Headspace Gas.............42 Table 10. Typical LC/MS/MS Operational Parameters for Analysis of 8-2 FTA, 8-2 FTUA,
PFOA, PFNA, PFDA and PFUA in Soil Extracts........................................................................ 43 Table 11. Typical LC/MS/MS Operational Parameters for Analysis of 8-2 FTOH in Soil Extracts........44 Table 12. Typical LC/MS/MS Operational Parameters for Analysis of 7-3 Acid and 7-2 sFTOH in Soil
E x tra c ts ............................................................................................................................................... 45
FIGURES
Figure 1. Method flowchart for the analysis o f 8-2 FTOH in the headspace of soil test chambers.........46
Figure 2. Method flowchart for the analysis o f 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in soil extracts............................................................. 47
Figure 3. Representative ion chromatogram for 8-2 FTOH in a low-level (25.0 ng a.i./mL) calibration standard by GC/MS.................................................................................................. 48
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TABLE OF CONTENTS - Continued -
Project No.: 112E-108
Figure 4. Representative ion chromatogram for 8-2 FTOH in a high-level (500 ng a.i./mL) calibration standard by GC/M S......................................................................................................49
Figure 5. Representative ion chromatogram for 8-2 FTOH in a low-level (10.0 ng a.i./mL) calibration standard by LC/MS/MS................................................................................................50
Figure 6. Representative ion chromatogram for 8-2 FTOH in a high-level (1000 ng a.i./mT.) calibration standard by LC/MS/MS................................................................................................51
Figure 7. Representative ion chromatogram for 7-2 sFTOH in a low-level (5.00 ng a.i./mL) calibration standard by LC/MS/MS................................................................................................52
Figure 8. Representative ion chromatogram for 7-2 sFTOH in a high-level (250 ng a.i./mL) calibration standard by LC/MS/MS................................................................................................53
Figure 9. Representative ion chromatogram for 8-2 FTA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standards by LC/MS/MS..................................................................................54
Figure 10. Representative ion chromatogram for 8-2 FTA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standards by LC/MS/MS..................................................................................55
Figure 11. Representative ion chromatogram for 7-3 Acid in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.................................................................................... 56
Figure 12. Representative ion chromatogram for 7-3 Acid in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS....................................................................................57
Figure 13. Representative ion chromatogram for 8-2 FTUA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS................................................................. 58
Figure 14. Representative ion chromatogram for 8-2 FTUA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS................................................................. 59
Figure 15. Representative ion chromatogram for PFOA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS................................................................. 60
Figure 16. Representative ion chromatogram for PFOA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS................................................................. 61
Figure 17. Representative ion chromatogram for PFNA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.........................................
62
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TABLE OF CONTENTS
- Continued -
Figure 18. Representative ion chromatogram for PFNA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.................................................................. 63
Figure 19. Representative ion chromatogram for PFDA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.................................................................. 64
Figure 20. Representative ion chromatogram for PFDA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.................................................................. 65
Figure 21. Representative ion chromatogram for PFUA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by GC/MS ........................................................................66
Figure 22. Representative ion chromatogram for PFUA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.................................................................................... 67
Figure 23. Representative calibration curve for 8-2 FTOH by G C /M S........................................................ 68
Figure 24. Representative calibration curve for 8-2 FTOH by LC/MS/MS...................................................69
Figure 25. Representative calibration curve for 7-2 sFTOH by LC/MS/MS.................................................70
Figure 26. Representative calibration curve for 8-2 FTA by LC/M S/M S.....................................................71
Figure 27. Representative calibration curve for 7-3 Acid by LC /M S/M S.................................................... 72
Figure 28. Representative calibration curve for 8-2 FTUA by LC/M S/M S..................................................73
Figure 29. Representative calibration curve for PFOA by LC/MS/MS .-....................................................... 74
Figure 30. Representative calibration curve for PFNA by LC/MS/MS......................................................... 75
Figure 31. Representative calibration curve for PFDA by LC /M S/M S........................................................ 76
Figure 32. Representative calibration curve for PFUA by LC/MS/MS......................................................... 77
Figure 33. Representative ion chromatogram for 8-2 FTOH in the sampled headspace (Ci8 cartridge extract) from a Day 0 untreated control soil by GC/MS ............................................................. 78
Figure 34. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 untreated control soil by LC/MS/MS..........................................................................................79
Company Sanitized. Does not contain TSCA CBI.
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Project No.: 112E-108
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- Continued -
Figure 35. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Day 0 untreated control soil by LC/M S/M S............................................................................................80
Figure 36. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0
.
untreated control soil by LC/M S/M S............................................................................................81
Figure 37. Representative ion chromatogram for 7-3 Acid in the soil extract from a Day 0 untreated control soil by LC/M S/M S......... a............................................................................... 82
Figure 38. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 untreated control soil by LC/M S/M S............................................................................................83
Figure 39. Representative ion chromatogram for PFOA in the soil extract from a Day 0 untreated control soil by LC/MS/MS.............................................................................................................84
Figure 40. Representative ion chromatogram for PFNA in the soil extract from a Day 0 untreated control soil by LC/MS/MS............................................................................................................85
Figure 41. Representative ion chromatogram for PFDA in the soil extract from a Day 0 untreated control soil by LC/MS/MS................ '............................................................................................86
Figure 42. Representative ion chromatogram for PFUA in the soil extract from a Day 0 untreated control soil by LC/MS/MS............................................................................................................ 87
Figure 43. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Cig cartridge extract) from a Month 24 untreated control soil by GC/M S.......................................................88
Figure 44. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 24 untreated control soil by LC/M S/M S........................................................................................... 89
Figure 45. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Month 24 untreated control soil by LC/M S/M S..................... ;..................................................................... 90
Figure 46. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 24 untreated control soil by LC/M S/M S............................................................................................91
Figure 47. Representative ion chromatogram for 7-3 Acid in the soil extract from a Month 24 untreated control soil by LC/M S/M S........................................................................................... 92
Figure 48. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 24 untreated control soil by LC/MS/MS........................................................................................ 93
Company Sanitized. Does not contain TSCA CBI.
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Project No.: 112E-108
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Figure 49. Representative ion chromatogram for PFOA in the soil extract from a Month 24 untreated control soil by LC/M S/M S.............................................................................................94
Figure 50. Representative ion chromatogram for PFNA in the soil extract from a Month 24 untreated control soil by LC/M S/M S.............................................................................................95
Figure 51. Representative ion chromatogram for PFDA in the soil extract from a Month 24 untreated control soil by LC/M S/M S.............................................................................................96
Figure 52. Representative ion chromatogram for PFUA in the soil extract from a Month 24 untreated control soil by LC/M S/M S.............................................................................................97
Figure 53. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C18 cartridge extract) from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by GC/MS................................. ....................................................................................98
Figure 54. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS............... 99
Figure 55. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS............. 100
Figure 56. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS............. 101
Figure 57. Representative ion chromatogram for PFOA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.............102
Figure 58. Representative ion chromatogram for PFNA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.............103
Figure 59. Representative ion chromatogram for PFDA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.............104
Figure 60. Representative ion chromatogram for PFUA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.............105
Figure 61. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Ci8cartridge extract) from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by GC/MS............................................................................................................... 106
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Project No.: 112E-108
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Figure 62. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 fig PFCAs/kg) control soil by LC/MS/MS.............107
Figure 63. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.............108
Figure 64. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.............109
Figure 65. Representative ion chromatogram for PFOA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.............110
Figure 66. Representative ion chromatogram for PFNA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.............I l l
Figure 67. Representative ion chromatogram for PFDA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.............112
Figure 68. Representative ion chromatogram for PFUA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.............113
Figure 69 Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Qg cartridge extract) from a Day 0 sterile soil treated with 200 mg/kg H-26634 by GC/MS...................... 114
Figure 70. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 sterile soil treated with 200 mg/kg H-26634 by LC/M S/M S............................................................... 115
Figure 71. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 sterile
soil treated with 200 mg/kg Q
^by LC/M S/M S.................................................................116
Figure 72. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 sterile soil treated with 200 mg/kgQ J b y LC/M S/M S.................................................................117
Figure 73. Representative ion chromatogram for PFOA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg_ J y LC/M S/M S................................................................ 118
Figure 74. Representative ion chromatogram for PFNA in the soil extract from a Day 0 sterile
soil treated with 200 mg/kg
^ by LC/M S/M S............................................................... 119
Figure 75. Representative ion chromatogram for PFDA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg' J b y LC/MS/MS.............................................................. 120
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Project No.: 112E-108
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Figure 76. Representative ion chromatogram for PFUA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg [_ 3 by LC/MS/MS................................................................. 121
Figure 77. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Cig cartridge
extract) from a Month 12 sterile soil treated with 200 mg/kg
J b y GC/MS................122
Figure 78. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 12 sterile
soil treated with 200 mg/kg
Jby LC/MS/MS................................................................ 123
Figure 79. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 12 sterile soil treated with 200 mg/kgC 3 by LC/MS/MS................................................................. 124
Figure 80. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 12 sterile soil treated with 200 m g/kg 3 by LC/MS/MS...................................................................125
Figure 81. Representative ion chromatogram for PFOA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg 3 ^ y LC/MS/MS..................................................................126
Figure 82. Representative ion chromatogram for PFNA in the soil extract from a Month 12 sterile soil treated with 200 m g/kg^ 3 by LC/MS/MS................................................................... 127
Figure 83. Representative ion chromatogram for PFDA in the soil extract from a Month 12 sterile
soil treated with 200 m g/kg^
])by LC/MS/MS................................................................. 128
Figure 84. Representative ion chromatogram for PFUA in the soil extract from a Month 12 sterile soij treated with 200 mg/kg Q 3by LC/MS/MS..................................................................129
Figure 85. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C18 cartridge
extract) from a Day 0 live soil treated with 200 mg/kg
by G C /M S..........................130
Figure 86. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 live
soil treated with 200 m g/kg
3^y LC/MS/MS..................................................................131
Figure 87. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Day 0 live
soil treated with 200 mg/kgQ
3by LC/MS/MS...................................................................132
Figure 88. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 live
soil treated with 200 m g/kg
j)by LC/MS/MS..................................................................133
JFigure 89. Representative ion chromatogram for 7-3 Acid in the soil extract from a Day 0 live
soil treated with 200 mg/kg
by LC/MS/MS..................................................
134
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Project No.: 112E-108
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Figure 90. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 live
soil treated with 200 mg/kg
*3by LC/MS/MS................................................................ 135
Figure 91. Representative ion chromatogram for PFOA in the soil extract from a Day 0 live
soil treated with 200 mg/kg [
^by LC/MS/MS....................
136
Figure 92. Representative ion chromatogram for PFNA in the soil extract from a Day 0 live
soil treated with 200 mg/kg[
^by LC/MS/MS................................................................ 137
Figure 93. Representative ion chromatogram for PFDA in the soil extract from a Day 0 live soil treated with 200 m g / k g "3 by LC/MS/MS............................................................... 138
Figure 94. Representative ion chromatogram for PFUA in the soil extract from a Day 0 live soil treated with 200 m g /k ^ ~0by LC/MS/MS........................................ ........................139
Figure 95. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Ci8 cartridge
extract) from a Month 24 live soil treated with 200 m g/kgf
GC/M S................... 140
Figure 96. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 24 live
soil treated with 200 mg/kg [
^ by LC/MS/MS............................................................... 141
Figure 97. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Month 24 live
soil treated with 200 mg/kg
3 by LC/MS/M s ............................................................... 142
Figure 98. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 24 live soil treated with 200 m g/kg^' ' ^yL O M S /M iS ............................................................... 143
Figure 99. Representative ion chromatogram for 7-3 Acid in the soil extract from a Month 24 live
soil treated with 200 mg/kg
)jy LC/MS/MS................................................................144
Figure 100. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 24 live soil treated with 200 m g/kg ]) by LC/MS/MS...............................................................145
Figure 101. Representative ion chromatogram for PFOA in the soil extract from a Month 24 live
soil treated with 200 mg/kg
Jb y LC/MS/MS...............................................................146
Figure 102. Representative ion chromatogram for PFNA in the soil extract from a Month 24 live
soil treated with 200 mg/kg Q
}by LC/MS/MS...........>................................................. 147
Figure 103. Representative ion chromatogram for PFDA in the soil extract from a Month 24 live soil treated with 200 mg/kg Q Joy LC/MS/MS............................................................ 148
Company Sanitized. Does not contain TSCA CBI.
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Project No.: 112E-108
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TABLE OF CONTENTS
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Figure 104. Representative ion chromatogram for PFUA in the soil extract from a Month 24 live
soil treated with 200 mg/kgfy
_3by LC/MS/MS.....................................................
APPENDICES
Appendix I. Measured Concentration o f Selected Analytes (pg/kg Soil Dry W eight)................
Appendix II. Measured Concentration of Selected Analytes (pmol/kg Soil Dry W eight)...........
Appendix III. Example Calculations...................................................................................................
Appendix IV. Duration of Incubation.................................................................................................
149
150 223 296 298
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STUDY INFORMATION
Study Initiation Date: Experimental Start Date: Experimental Termination Date: Study Completion Date:
10 March 2005 11 March 2005 07 September 2007 17 March 2008
p. 17 Project]*,, m s -io s
Study Director: Sponsor: Sponsor Representatives: Study Personnel:
Edward C. Schaefer
E.I. du Pont de Nemours and Company
William Berti and Bogdan Szostek
Edward C. Schaefer, B.S., Director of Biodegradation Raymond L. Van Hoven, Ph.D., Scientist, Chemistry Joel I. Stenzel, B.S., Senior Chemist R. Scott Flaggs, B.S., Biologist, Biodegradation Abul Siddiqui, B.S., Scientist, Biodegradation Frank Lezotte, B.S., Senior Chemist, Chemistry David L. Weiss, M.S., Chemist, Chemistry William Schutt, B.S., Chemist, Chemistry John R. Murrell, B.S., Senior Chemist Willard B. Nixon, Ph.D., Director o f Chemistry Henry O. Krueger, Ph.D., Director of Aquatic
Toxicology/Terrestrial Plants and Insects Molly E. Matthews, Biologist, Biodegradation Jon A. MacGregor, B.S., Scientist
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INTRODUCTION
This report describes a laboratory research study conducted to assess aerobic and anaerobic
transformation o f
Din soil systems. This study was conducted by Wildlife International, Ltd.
for E.I. du Pont de Nemours and Company at the Wildlife International, Ltd. research facility in
Easton, Maryland. The raw data generated by Wildlife International, Ltd. and the original final report
final report will be archived by the study sponsor. A certified copy will be retained under Project
Number 112E-108 in the archives located at on the Wildlife International, Ltd. site.
OBJECTIVE The objective of the research study was to assess the potential for transformation of the test substance in aerobic and anaerobic soils.
EXPERIMENTAL DESIGN The test was conducted with four test soils under aerobic conditions. The study was initially designed to set-up to also include four soils under anaerobic conditions, however a check of the system indicated that the test chamber closures did not exclude air. The systems initially started under anaerobic conditions up to and including month 6 are included in this report because they were extracted and analyzed as planned.
Four groups were established for each soil type and condition (aerobic or anaerobic): (1) untreated (control) live soil; (2) 200 mg test substance/kg dw treated live soil; (3) 200 mg test substance/kg dw treated sterile soil; (4) 250 pg 8-2 telomer B alcohol/kg dw treated sterile soil; 10 pg 8-2 telomer B acid /kg dw; 10 pg 8-2 unsaturated telomer B acid/kg dw; and 10 pg perfluorooctanoic acid/kg dw, heptadecafluorononaoic acid, nonadecafluorodecanoic acid, and perfluoroundecanoic acid at 10 pg/kg dw all added to sterile soil. The test systems reported here were incubated in sealed containers at approximately 20C for at approximately 24 months. Two incubation chambers from each of the groups were removed at appropriate time intervals and the soil samples were extracted and analyzed for potential metabolites. In addition to the day zero samples, eight samplings were performed with all of the groups. Three additional sampling intervals were performed with only the untreated control (1) and 200 mg test substance/kg dw treated live soil (2) groups.
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Additional untreated and test substance treated soil chambers were prepared for use as matrix
fortification samples, biomass determinations, viability controls and to monitor aerobic conditions as
necessary.
The test groups were prepared using 25 grams (dry weight equivalent) of soil. While this amount o f soil is less than the 50 to 200 grams specified in the OECD 307 guideline, it was chosen to allow for 1) the extraction of the entire test chamber contents and internal surfaces; 2) sufficient headspace to minimize the need for active aeration which could result in a loss of potential transformation products.
METHODS AND MATERIALS The test system and study conditions are based on the OECD Guideline for Testing of Chemicals, Guideline 307, Aerobic and Anaerobic Transformation in Soil (1). As this guideline is designed for low molecular weight substances, but not for polymers, adaptations o f the method may be necessary for feasibility reasons. Nevertheless, the study was carried out in such a way that the recommendations given in the guideline were followed as closely as possible.
Test Substance The following substance was used in this study:
rot ) Date received: 2 November 2004
f'
Wildlife International Ltd. ID: 6918
1
Measured Analytes
Name: 8-2 Telomer B Acid, 8-2 Fluorotelomer Acid
Synonym used in Report: 8-2 FTA
CAS Name: Decanoic acid, 3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-heptadecafluoro-(8CI,9CI) CAS Number: 27854-31-5
Molecular Weight: 478.1 g/mole
ID: H-26210
Date received: 1 September 2004
.
Expiration/reanalysis date: 12 October 2007
Purity: 97%; 3% 2H-Hexadecafluoro-2-Decanoic Acid
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Description: Solid Wildlife International Ltd. ID: 6826
Name: 8-2 Telomer B Alcohol, 8-2 Fluorotelomer Alcohol Synonym used in Report: 8-2 FTOH CAS Name: 1-Decanol, 3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-heptadecafluoro- (7CI, 8CI, 9CI) CAS Number: 678-39-7 Molecular Weight: 464.11 g/mole ID: H-24691 Date received: 1 September 2004 Expiration/reanalysis date: 12 October 2007 Purity: 99.2% Description: Solid Wildlife International Ltd. ID: 6828
Name: 8-2 Unsaturated Telomer B Acid, 8-2 Fluorotelomer Unsaturated Acid Synonym used in Report: 8-2 FTUA CAS Name: 2-Decenoic acid, 3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-hexadecafluoro- (9CI) CAS Number: 70887-84-2 Molecular Weight: 458.1 g/mole ID: H-26211 Date received: 1 September 2004 Expiration/reanalysis date: 12 October 2007 Purity: 98%; 0.5% 8-2 FTOH; 1.5% unknown Description: Solid Wildlife International Ltd. ID: 6829
Name: N-Perfluorooctanoic Acid Synonym used in Report: PFOA CAS Name: Octanoic acid, pentadecafluoro- (8CI, 9CI) CAS Number: 335-67-1 Molecular Weight: 414.07 g/mole ID: H-24749 Date received: 10 September 2004 Expiration/reanalysis date: 12 October 2007 Purity: 99.2% Description: Solid Wildlife International Ltd. ID: 6849
Name: Heptadecafluorononaoic Acid, Perfluorononanoic Acid Synonym used in Report: PFNA
CAS Name: Nonanoic acid, heptadecafluoro-(8CI, 9CI) CAS Number: 375-95-1 Molecular Weight: 464.08 g/mole ID: H7568 Date received: 30 November 2004 Expiration/reanalysis date: 12 October 2007
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Purity: 99+% Description: Solid Wildlife International Ltd. ID: 6944
,
Name: Nonadecafluorodecanoic Acid, Perfluorodecanoic Acid Synonym used in Report: PFDA CAS Name: Decanoic acid, nonadecafluoro (6CI, 7CI, 8CI, 9CI)
CAS Number: 335-76-2 Molecular Weight: 514.08 g/mole
ED: R11K Date received: 30 November 2004 Expiration/reanalysis date: 12 October 2007 Purity: 99+% Description: Solid Wildlife International Ltd. ID: 6943
Name: Perfluoroundecanoic Acid, Perfluoroundecanoic Acid Synonym used in Report: PFUA CAS Name: Undecanoic acid, heneicosafluoro-,ammonium salt (9CI)
CAS Number: 4234-23-5 Molecular Weight: 564.09 g/mole ID: Q30G Date received: 30 November 2004 Expiration/reanalysis date: 12 October 2007
Purity: 99+% Description: Solid Wildlife International Ltd. ID: 6945
Name: 2H, 2H, 3H, 3H Pentadecafluoro-Decanoic acid
Synonym used in Report: 7-3 Acid CAS Name: Decanoic acid, 4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-pentadecafluoro-
CAS Number: 812-70-4
.
Molecular Weight: 442.12 g/mole
ID :26833
Date received: 20 April 2006
Expiration/reanalysis date: Not provided
Purity: Not provided
Description: Solid Wildlife International Ltd. ID: 7592
Name: 1-Perfluoro heptyl ethanol, 7-2 Secondary Fluorotelomer Alcohol
Synonym used in Report: 7-2 sFTOH CAS Name: Not provided CAS Number: Not provided Molecular Weight: 414.11 g/mole ID :27497 Lot: 72SFTOHGA05
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Date received: 20 April 2006 Expiration/reanalysis date: Not provided Purity: Not provided Description: Liquid Wildlife International Ltd. ID: 7593
Internal Standards
Name: N-Perfluorooctanoic Acid [l,2-di-13C] Synonym used in Report: 2-13CPFOA CAS Name: NA CAS Number: NA ID: H-25617 Dates received: 1 September 2004 and 20 April 2006 Expiration/reanalysis date: 12 October 2007 and 31 January 2007 Purity: 96.4% (not given for WIL 7591) Description: Solid Wildlife International Ltd. ID: 6827, 7591 Standard Reanalysis Date: August 2007 Purity: Confirmed Expiration Date: August 2010
Name: ID, ID, 2D, 2D, 3-13C-Heptadecafluorodecanol Synonym used in Report: HDFD CAS Name: NA CAS Number: NA ID: H-25433 Dates received: 10 September 2004 and 20 April 2006 Expiration/reanalysis date: 12 October 2007 Purity: 100% (not given for WIL 7590) Description: Solid Wildlife International Ltd. IDs: 6850, 7590
p. 22 Project No.: 112E-108
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Test Soils Four freshly collected United States (U.S.) soils were used to evaluate the test substance
under aerobic and anaerobic conditions. The soils were collected from each of the following soil orders:
Soil O rder
Percent of US Land*
Alfisol
13
Mollisol
. 25
Inceptisol
16
Ultisol
13
Percent o f total land in the U S, from Foth, H.D. 1990. Fundamentals o f Soil Science 8 ed. John W iley & Sons, N ew York)
The soils were processed between 6 and 22 days after collection. Processing included removing vegetation, larger soil fauna, stones and passing the soil through a 2 mm sieve. The soil used in the sterile groups was sterilized by cobalt irradiation. The soils were stored in the dark at 4 2 C.
Characterization of the soils was conducted by Agvise Laboratories (Northwood, North
Dakota, USA). The following is a list of the minimum physicochemical properties of the soil.
Texture (i.e., percentage of sand, silt and clay) (NUT.02.06) pH (NUT.02.05) Organic carbon (NUT.20.20) Bulk density (NUT.02.10) Field moisture capacity (% moisture at 1/3 bar) Cation exchange capacity (NUT.02.03)
The percent moisture and water holding capacity (ISO 14240-2; Annex A) of each of the soil
types was determined. In addition, the microbial biomass (ISO 14240-2; fumigation-extraction) o f a
live sample of each soil type was determined. The soil microbial biomass was determined at the beginning of the test prior to adding the test substance and in test and control soil samples at 4, 6 and
12 months using the fumigation-extraction method.
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Test Apparatus and Conditions The test chambers were 250 mL glass serum bottles (Wheaton, Milleville NJ). The chambers
were sealed with butyl rubber septa (Wheaton, Milleville NJ) that were covered with aluminum foil and aluminum crimp caps (Wheaton, Milleville NJ). The test chambers were identified by project number, test substance ID, test concentration, and unique identifier and incubated statically at approximately 20 2C.
Soil Pre-Incubation The soil moisture of each test soil content was adjusted to 40 to 60% o f water holding
capacity (which is equivalent to a pF of between 2.0 and 2.5, or from 0.1 to 0.33 bar). The test soil then was allowed to warm to the test temperature for at least 2 days. A pre-incubation period of at least 7 days for the aerobic soils and at least 14 days for the anaerobic soils was performed under the appropriate conditions (i.e. temperature, soil moisture content, aerobic or anaerobic). Approximately 25 grams dry weight equivalent of test soil was added to the test chambers. Sterile soil treatments were dosed at approximately 200 mg/kg (dw) with both chloramphenicol (Aldrich, 98%, CAS 56-75 7) and cycloheximide (Sigma, min. 94%, CAS 66-81-9) to inhibit microbial growth. The chambers to be incubated under aerobic conditions were sealed with aluminum foil-lined septa and incubated. The chambers to be incubated under anaerobic conditions were flushed with anaerobic mixed gas, sealed with aluminum foil-lined septa and incubated. The soil moisture content was maintained in the optimal microbial growth range of 40 to 60% water holding capacity (WHC). The soil moisture content o f each chamber was checked and adjusted, if necessary, once during the pre-incubation period by weighing the test vessels. Sterile-filtered demineralized water (degassed sterile-filtered demineralized water for the anaerobic soils) was added as necessary to compensate for water losses.
Preparation of the Test Chambers The soil moisture content was checked and adjusted, if necessary, prior to the addition of the
test substance. Sterile-filtered demineralized water (degassed sterile-filtered demineralized water for the anaerobic soils) was added as necessary to compensate for water. Anaerobic treatments and controls were prepared within an anaerobic environment and stored in a temperature controlled room. The test substance was applied to the soil within the test chambers as described below.
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Background Blank Control: Soils with no test substance added was tested in duplicate to check for background concentrations of analytes (see Sampling and Measurements). The entire bottle was extracted after a sample of the headspace had been taken.
Tffst Substance: The test substance dosed treatments were prepared by dosing approximately 25 grams (dry weight equivalent) of soil with sufficient test substance to deliver 200 mg/kg dry weight. The test substance was administered by direct weight addition. The test chambers were sealed with septum lined with aluminum foil, mixed and incubated. The entire bottle was extracted after a sample of the headspace had been taken.
Abiotic Controls: The abiotic controls were prepared by dosing approximately 25 grams (dry weight equivalent) of 6Co-sterilized soil with sufficient test substance to deliver 200 mg/kg dry weight. The test substance was administered by direct weight addition. The entire bottle was extracted after a sample of the headspace had been taken.
Spike Recovery Controls: The spike recovery controls were prepared by dosing approximately 25 grams (dry weight equivalent) of Co-sterilized soil with appropriate volumes of 8-2 FTOH stock (250 )ig/mT. in ethanol) and fluorinated acid stocks (10 pg/mL in water). The stocks were injected directly into the soil using a glass microsyringe. The test chambers were immediately sealed with septum lined with aluminum foil and the contents of the chambers were mixed. The entire bottle was extracted after a sample of the headspace had been taken.
Viability Controls (untreated!: The metabolic activity of untreated test soil was assessed on a monthly basis. Duplicate incubation chambers for each test soil and condition (aerobic and anaerobic) were dosed at approximately 100 mg/kg dw with a combination of radiolabeled and non-labelled glucose. The evolved 14C 0 2 (aerobic and anaerobic conditions) and 14OELt (anaerobic conditions) was measured and the percent biodegradation calculated.
Aerobic Controls (Treated!: The aerobic controls were prepared by dosing approximately 25 grams (dry weight equivalent) of soil with sufficient test substance to deliver 200 mg/kg dry weight. The test substance was administered by direct weight addition. The test chambers were sealed with septum lined with aluminum foil, mixed and incubated. The oxygen content of a sacrificial chamber was measured at monthly intervals.
Maintenance of Test Chambers The soil moisture content was maintained in the optimal microbial growth range of 40 to 60%
water holding capacity (WHC; a pF of between 2.0 and 2.5, or from 0.1 to 0.33 bar). The soil moisture content was initially checked and adjusted, if necessary, at 1 to 2 week intervals by weighing the test vessels but was changed to monthly intervals based on the observed weight losses. Adding sterile-filtered demineralized water (degassed sterile-filtered demineralized water for the anaerobic soils) compensated for water losses.
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The oxygen content in the headspace of two aerobic control chambers was assessed on a monthly basis to ensure aerobic conditions. If the mean measured oxygen content within the control chambers was less than 19%, aerobic test chambers was aerated with filter-sterilized, oil-free air.
Test chambers did not have to be opened to add water or for monitoring purposes. Soil moisture content was adjusted by injecting the appropriate amount of sterile water through the septa and onto soil. The headspace oxygen content was measured by inserting the probe of an oxygen analyzer through the septa and into the headspace of the aerobic control chamber.
Sampling and Measurements
Duplicate chambers from each of the control, treated and spike recovery soils were extracted and analyzed. Sampling intervals were at 0, 1 and 2 weeks and 1, 2, 4, 6, 9 12, 15, 18 and 24 months
for aerobic. Potential volatile transformation products in the headspace of the soils chambers were collected using a C l8 cartridge. At each sampling time prior to opening the test vessel, the septum was
pierced using a needle connected to a C l8 cartridge and syringe. A 20-mL volume of headspace gas from within the chamber was pulled through the C l8 cartridge using the attached syringe. The Cl 8 was
subsequently extracted with ACN and analyzed. Sample extracts were stored at -10C or lower if analysis is delayed more than 24 hours. Extracts were analyzed for:
1. 8-2 FTOH 2. 8-2 FTA 3. 8-2 FTUA 4. PFOA 5. PFNA 6. PFDA 7. PFUA 8. 7-2 sFTOH 9. 7-3 Acid
Quality Criteria
Recovery for a given sampling time point should range between 70 to 120% for the analysis of
the 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFNA, PFDA, PFUA, and PFOA from the spike recovery control samples. These ranges should be interpreted as targets and should not be used as criteria for acceptance o f the test.
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Viability Assessments
On a monthly basis, duplicate incubation chambers for each test soil and condition were dosed with 1 mL of the stock solution containing both radiolabelled and non-labelled forms of d-glucose which resulted in a concentration of approximately 3000 mg/kg dw. The headspace of each of the aerobic test chambers was continuously purged at an air-flow rate of approximately 1 bubble per second. The air exiting the test chambers was be passed through a series of gas washing bottles with at least one containing approximately 100 mL of 1.5 N KOH. The headspace of each of the anaerobic chambers was continuously purged with a flow of nitrogen and passed through a gas collection system consisting of two sets of C 0 2 traps and a combustion apparatus. The displaced gases initially passed through one empty bottle followed by two bottles each containing approximately 100 mL of 1.5N KOH (C 02 trapping solution) followed by another empty bottle. The gas was combined with a flow of oxygen and channeled through a quartz column that was packed with cupric oxide and maintained at approximately 800C in a tube furnace to combust methane to C 0 2. The gas exiting the combustion column was passed through an empty bottle followed by two additional C 0 2 traps. The C 02 traps connected to the aerobic chambers and the 1st C 0 2 trap in each set (before and after furnace) of the anaerobic mineralization apparatus were removed for analysis and replaced at least weekly. Three replicate 1 mL aliquots of C 0 2 trapping solution were analyzed for radioactivity by liquid scintillation counting (LSC; Packard Tri-Carb Liquid Scintillation Counter). The assessment was terminated when 50% of the initial activity was recovered as mineralization products or after 28 days.
RESULTS Test Conditions
Room temperatures during the acclimation period for all four soils ranged from 18 to 22 C. Temperatures recorded during the test for all four soils ranged from 17 to 25 C. Incubation temperatures outside of the range 18 to 22 C were observed three times over the duration of the study.
The frequency of measurements performed on the test soils is presented in Table 1. The moisture contents of the soils were adjusted as needed based on soil moisture content determinations. Each vessel required water additions at least once during the initial 12-month test period, but no additions were required for shorter incubation times. The maximum amounts of water added to any vessel over 12
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and 24 month periods were 1.2 and 3.9 mL, respectively. The oxygen content in the headspace of the two aerobic control chambers used to assess aerobic conditions is presented in Table 2.
Characterization of Soils The test soils were characterized before the start o f acclimation, and the results are presented in
Table 3. The results of the viability assessments are presented in Tables 4-7. The results of microbial biomass determinations performed at the start of the test, during the test, and at the end o f the test are presented in Table 8.
Sample Processing and Analysis Soil chambers were either processed immediately upon receipt or removed from frozen storage
and prepared for analysis of the candidate analytes 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA.
Headspace Analyses Prior to opening the soil chambers, a 20-mL volume o f headspace gas was sampled from each
chamber for 8-2 FTOH using a gas-tight syringe (Becton Dickinson, 10 mL with Luer-LokTM tip), hypodermic needle (Becton Dickinson, 18G 154) and a C]8 cartridge (600 mg, Alltech Maxi-Clean). Each sampled C!8 cartridge was extracted with 10 mL of acetonitrile (Burdick & Jackson, HPLC-Grade, CAS No. 75-05-8). The extracts were fortified with 100 pL of a 0.100 mg HDFD/mL internal standard in acetonitrile to yield an internal standard concentration of 100 ng/mL in each extract. The fortifications were performed using a gas-tight syringe (Hamilton 81000 Series). An aliquot o f each sample extract was transferred into a glass autosampler vial, sealed and analyzed for 8-2 FTOH by GC/MS. A method outline and typical instrumental conditions for analysis of 8-2 FTOH in the headspace of the soil chambers are provided in Figure 1 and Table 9, respectively.
Soil Analyses Following headspace sampling, the crimp seal from each soil chamber was removed and the
aluminum foil liner was placed inside the test vessel. A 125-milliliter aliquot of acetonitrile extraction solvent (measured with a graduated cylinder) followed by a 10-milliliter aliquot of 200 mM sodium hydroxide (measured with a serological pipette and prepared with Aldrich, Lot No. 14227TO NaOH or Fisher, Lot No. 926471 and Burdick & Jackson HPLC-Grade reagent water, CAS
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No. 7732-18-5) were added to each test vessel containing the soil sample. The acetonitrile extraction solvent contained 100 ng/mL of HDFD internal standard and was prepared by fortifying four liters of acetonitrile measured with a graduated cylinder with 400 pL of a 1.00 mg HDFD/mL primary stock' in isopropyl alcohol (Burdick & Jackson, HPLC-Grade, CAS No. 67-63-0) and a gas-tight syringe. The vessels were re-sealed and placed onto a gyratory shaker table (New Brunswick Scientific, Model G10) set for 250 rpm for three hours at room temperature.
The vessels were removed from the gyratory table and allowed to settle for several minutes at ambient temperature. Using a volumetric pipette, a 10-mL aliquot o f the extract from each vessel was transferred into a separate polypropylene centrifuge tube (Nalgene, 50-mL polypropylene Oak Ridge type, Catalog No. 3119-0050) containing 25 milligrams of ENVI-Carb (Supelco, 120/400). The test vessel, including the remainder of the extract, was replaced into frozen storage (operating range -10 to -20C). The extract aliquots were centrifuged (Sorvall RC5BPlus fitted with Sorvall SS-34 rotor) for 10 minutes at 12000 rpm (17000 g) and 20 degrees C.
An aliquot of supernatant was diluted with reagent water containing 1.00 ng/mL of 2-13C PFOA internal standard and in the proportions 1 part extract: 3 parts dilution water and the diluted extract was analyzed for 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by HPLC tandem mass spectrometry (LC/MS/MS). The dilutions were performed directly in HPLC autosampler vials using calibrated micropipettes. The dilution water was freshly prepared by fortifying 100 mL of reagent water with 100 pL o f a 2-13C PFOA secondary stock in acetonitrile using a borosilicate glass, Class A volumetric flask and either a gas-tight syringe or calibrated micropipettes.
A second aliquot of supernatant was diluted with reagent water containing 1.00 ng/mL of HDFD internal standard and in the proportions 1 part extract: 1 part dilution water and the diluted extract was analyzed for 8-2 FTOH by LC/MS/MS. The dilutions were performed directly in HPLC autosampler vials using calibrated micropipettes. The dilution water was freshly prepared by fortifying 100 mL of reagent water with 100 pL of a HDFD secondary stock in acetonitrile using a borosilicate glass, Class A volumetric flask and gas-tight syringe. The remainder of the soil extract supernatant was decanted into glass scintillation vials (Fisher, 20-mL disposable, Catalog No. 03-337 7) and placed in frozen storage.
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At the request of the Sponsor, two additional analytes, 7-2 sFTOH and 7-3 Acid, were screened for in selected stored soil extracts collected prior to the Month 18 sampling interval using LC/MS/MS. The earlier sampling intervals included Day 0 and Months 2, 6, 9, 12 and 15. 7-2 sFTOH and 7-3 Acid were also screened for in soil extracts generated concurrent with the Months 18 and 24 sampling intervals. The soil treatments selected for the screen included one untreated control soil replicate and the live treated soil replicates in each o f the four soil types. Three different sample preparation regimes were used. For concurrent analyses of 7-2 sFTOH and 7-3 Acid, an aliquot of soil extract was diluted with reagent water containing 100 ng/mL of HDFD internal standard and 1.00 ng/mL of 2-13C PFOA internal standard in the proportions 1 part extract: 3 parts dilution water. This preparation was used for Month 18 and batch analyses on the earlier sampling intervals, except Day 0. For 7-2 sFTOH analyses only, an aliquot o f soil extract was diluted with reagent water containing 1.00 ng/mL of HDFD internal standard and in the proportion of 1 part extract: 3 part dilution water. For 7-3 Acid analyses only, an aliquot o f soil extract was diluted with reagent water containing 1.00 ng/mL o f 2-13C PFOA internal standard and in the proportions 1 part extract: 3 parts dilution water. The latter two preparations were used for processing Day 0 and Month 24 samples where re-analyses due to instrumental or sample stability issues were required (e.g. 7-2 sFTOH stability in the dilutions was suspect). Each o f the dilution solvents in reagent water was freshly prepared before use.
A method outline for the analysis 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in soil extracts by LC/MS/MS is presented in Figure 2. Typical instrumental conditions for LC/MS/MS analysis of 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA are provided in Table 10. Typical instrumental conditions for LC/MS/MS analysis of 8-2 FTOH are provided in Table 11. Typical instrumental conditions for LC/MS/MS analysis o f 7-2 sFTOH and 7-3 Acid are provided in Table 12.
Stock and Calibration Standard Preparation Individual component primary stock solutions (1.00 mg/mL) were prepared for each analyte
and internal standard from the neat materials using an analytical balance and volumetric glassware. Individual component secondary stock solutions (100, 10.0, 1.00 and 0.100 pg/mL) were also prepared by serial dilution. The 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA, PFUA, 2-13C PFOA and HDFD (for 24 month interval only) primary stock solutions were prepared in acetonitrile.
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The 8-2 FTOH, 7-2 sFTOH and HDFD (except as noted for the 24 month interval) primary stock solutions were prepared in isopropyl alcohol. All secondary stocks were serially diluted with acetonitrile. The 100-pg/mL HDFD secondary stock solution was used to fortify the internal standard into soil extraction solvent, calibration standards containing 8-2 FTOH and 7-2 sFTOH and dilution solvents for analyses o f soil extracts for these analytes.
For GC/MS analyses of 8-2 FTOH in the headspace gas of soil test chambers, 8-2 FTOH calibration standards in acetonitrile (25.0, 50.0, 100, 250 and 500 ng 8-2 FTOH/mL) and each fortified at 100 pg HDFD/mL internal standard, were prepared from the appropriate individual component stocks. Representative ion chromatograms for 8-2 FTOH calibration standards by GC/MS are presented in Figures 3 and 4.
For LC/MS/MS analyses of 8-2 FTOH in soil extracts, 8-2 FTOH calibration standards in 50: 50 (v/v) acetonitrile: reagent water (10.0, 25.0, 50.0, 100, 250 500 and 1000 ng 8-2 FTOH/mL) and each fortified at 100 pg HDFD/mL internal standard, were prepared from the appropriate individual component stocks. Representative ion chromatograms for 8-2 FTOH calibration standards by LC/MS/MS are presented in Figures 5 and 6.
For 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA analyses of soil extracts, a 100[ig/mT. -combined stock of 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in acetonitrile was prepared from the individual component primary stocks. Secondary combined acid stocks in acetonitrile (10.0, 1.00 and 0.100 pg/mL) were prepared by serial dilution from the 100 pg/mL combined stock solution. Combined 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA calibration standards in 25: 75 (v/v) acetonitrile: reagent water (0.100, 0.250, 0.500, 0.750 and 1.00 ng/mT.) and each fortified with 0.75 ng 2-13C PFOA/mL internal standard were prepared from the 0.100-pg/mL combined stock. A 5.00ng/mL combined 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA calibration standard in 25: 75 (v/v) acetonitrile: reagent water was prepared from the 1.00 11g/mT. combined stock. All stock and calibration standards were stored under refrigerated (-4 degrees C) conditions when not in use.
For 7-3 Acid and 7-2 sFTOH analyses of Months 2, 6, 9, 12, 15, 18 and 24 (7-3 Acid only) soil extracts, two sets o f combined stocks were prepared. A 10-pg/mL combined stock o f 8-2 FTA,
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7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in acetonitrile was prepared from the individual component primary stocks. Secondary combined stocks in acetonitrile (1.00 and 0.100 pg/mL) were prepared by serial dilution from the 10.0-pg/mL combined stock. A 10.0-pg/mL combined stock of 8-2 FTOH and 7-2 sFTOH in acetonitrile was prepared from the individual component primary stocks. Calibration standards in 25: 75 (v/v) acetonitrile: reagent water containing 0.75 ng 2-13C PFOA/mL and 100 ng HDFD/mL internal standards were prepared from the 0.100 and 1.00-pg/mL combined perfluorinated carboxylic acid (PFCA) and 10.0-pg/mL combined telomer alcohol stock solutions, respectively. The nominal concentrations of 7-3 Acid and 7-2 sFTOH in the calibration standard set (7-3 Acid/7-2 sFTOH) were 0.100/5.00, 0.250/10.0, 0.500/25.0, 0.750/35.0, 1.00/50.0 and 5.00/250 ng/mL. Due to stability concerns, fresh preparations of 7-2 sFTOH calibration standards were prepared from the 10.0-pg/mL combined stock of 8-2 FTOH and 7-2 sFTOH in acetonitrile for analyses o f 7-2 sFTOH in Month 24 soil extracts. The nominal concentrations of 7-2 sFTOH were as specified above.
For 7-3 Acid analyses of Day 0 soil extracts, a combined calibration standard for set for 7-3 Acid and 7-2 sFTOH only was prepared as described above from individual component 7-3 Acid (0.100 and 1.00-ng/mL) and 7-2 sFTOH (10.0-ng/mL) secondary stock solutions. Only 7-3 Acid results were reported from this standard set due to a delay in instrumental analyses. For 7-2 sFTOH analyses, another set of standards was freshly prepared from the 10.0-pg/mL 7-2 sFTOH secondary stock solution. Representative ion chromatograms for 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA calibration standards by LC/MS/MS are presented in Figures 7-22.
Calibration Curves and Limits of Quantitation Calibration standard sets for a given analysis were injected at the beginning and end o f each
analytical sequence with one calibration standard injected after a maximum of every five study samples. For 8-2 FTOH analyses in the headspace o f test vessels by GC/MS, linear regression equations were generated using adjusted peak areas (for the internal standard response ratio) versus the respective concentration of the calibration standards. The concentration of 8-2 FTOH in the sampled headspace o f the soil test vessels was determined by substituting the peak area response ratios obtained for the samples into the applicable linear regression equation. The concentrations were converted to a mass basis using the appropriate volumes, weights and unit conversion factors. A representative calibration curve for 8-2 FTOH analyses by GC/MS is presented in Figure 23.
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For soil extract analyses of 8-2 FTOH, 7-2 sFTOH, 8-2 FTA,7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS, weighted linear regression equations were generated using peak area response ratios (analyte:intemal standard) versus the respective concentration ratios (analyte:intemal standard) of the calibration standards. The concentration of each analyte in the samples was determined by substituting the peak area response ratios obtained for the samples into the applicable linear regression equation. Representative calibration curves for 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA analyses by LC/MS/MS are presented in Figures 24-32, respectively.
The limits of quantitation (LOQ) for these analyses were defined on a low-level standard equivalent basis and were calculated for 8-2 FTOH in the sampled headspace and for each analyte in soil as the product of the low-level standard concentration and the dilution factor of the untreated control samples. The LOQ for 8-2 FTOH in a 20 mL headspace gas aliquot was 12.5 ng/mL sampled headspace gas, or 0.250 pg for a 20-mL sample. The LOQ was 2.0 pg/kg for soil analyses of 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA. The LOQs for soil analyses of 8-2 FTOH and 7-2 sFTOH were 100 and 100 pg/kg, respectively. Statistical derivation of the limits of detection (LOD) was completed for all analytes with the exception of 7-3 Acid and 7-2 sFTOH during method verification trials. The calculation for each analyte was based on three times the concentration equivalent of the standard deviation of five replicate analyses of a reagent blank. Method verification trials and derivation of statistical LODs for 7-3 Acid and 7-2 sFTOH was outside the scope of the study.
Measured concentrations of the analytes are presented in mg/kg soil dry weight and pmol/kg soil dry weight in Appendix I and II, respectively. Measured concentrations of 7-2 sFTOH and 7-3 Acid in the untreated soils were less than the LOQ and are not presented. Example calculations are presented in Appendix III. Representative chromatograms are presented for each of the four treatment groups in aerobic alfisol soil. Similar chromatographic characteristics were evident for the aerobic ultisol, mollisol and inceptisol soils.
Representative ion chromatograms for 8-2 FTOH by GC/MS in the sampled headspace of
untreated control, fortified control, sterile and live samples treated with
J from Day 0 of the
test are presented in Figures 33, 53, 69 and 85, respectively. Representative ion chromatograms for
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8-2 FTOH in the sampled headspace of untreated control and live samples treated with" Month 24 of the test are presented in Figures 43 and 95, respectively.
om
Representative ion chromatograms for 8-2 FTOH by GC/MS in the sampled headspace of
fortified control and sterile samples treated withC
from Month 12 of the test are presented in
Figures 61 and 77, respectively.
Representative ion chromatograms for 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS in soil extracts from untreated control samples from Day 0 and Month 24 of the test are presented in Figures 34-42 and 44-52, respectively.
Representative ion chromatograms for 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS in soil extracts from fortified control samples from Day 0 and Month 12 of the test are presented in Figures 54-60 and 62-68, respectively.
Representative ion chromatograms for 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA
and PFUA by LC/MS/MS in soil extracts from sterile samples treated with L
3frm Day 0 and
Month 12 of the test are presented in Figures 70-76 and 78-84, respectively.
Representative ion chromatograms for 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS in soil extracts from live samples treated with
Jfrom Day 0 and Month 24 of the test are presented in Figures 86-94 and 96-104, respectively.
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-34REFERENCE
Project No.: 112E-108
1 Organisation for Economic Cooperation and Development. April 2002. A ero b ic an d A n aerobic Transform ation in Soil. OECD Guideline 307.
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Table 1
Frequency of Measurements1
Interval
Activity
Biomass Soil moisture Headspace Soil Analytical
oxygen
metabolic
interval
content
activity
Day 0 ' Week 1
V
V
V V
Week 2
V
V
Week 4
VVVV
Week 6 Month 2
V VVVV
Month 3
VVV
Month 4
V
V
V
V
V
Month 5
VVV
Month 6
V
V
V
V
V
Month 7
VVV
Month 8
VVV
Month 9
VVVV
Month 10
VVV
Month 11
VVV
Month 12
V
V
V
V
V
Month 15
VV
Month 18
V
VV
Month 21
V
Month 23
V
Month 24
V
VV
'Additional chambers for the measurement of biomass and headspace oxygen content beyond the initial 12 month test
period were not prepared at the start of the study.
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Interval
Month 1 Month 2 Month 3 Month 4 Month 5 Month 6 Month 7 Month 8 Month 9 Month 10 Month 11 Month 12
-36-
Table 2
Test Chamber Headspace Oxygen Content
Alfisol 20.8 21.0 20.9 20.8 20.8 20.9 20.8 20.8 20.8 20.9 20.8 20.9
Average Oxygen Content (%)
Inceptisol
Mollisol
20.8 20.7
20.9 20.7
20.9 20.6
20.8 20.8
20.7 20.7
20.9 20.7
20.7 20.8
20.8 20.9
20.8 20.8
20.9 20.8
20.8 20.8
20.9 20.8
Ultisol 20.8 20.7 20.6 20.8 20.8 20.8 20.7 " 20.8 20.8 20.9 20.8 20.8
'
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-37Table 3
Soil label Texture class
Alfisol Inceptsol Ultisol Mollisol
Sandy loam Loam
Sandy loam Sandy clay loam
1- cation exchange capacity
^-^pH^^j^ljo^wateyatr
Selected Soil Properties
Organic carbon content
%
1.4 2,0 2.9 2.1
Sand Silt Clay %
65 24 11 45 32 23 55 31 14 59 18 23
CEC1 meq/100 g
7.8 16.9 7.9 17.7
Water holding capacity pH2 %
5.8 54.5 6.6 43.3 4.8 59.3 6.9 63.2
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Table 4
Soil Viability by Glucose Mineralization: Alfisol
Month
Glucose Mineralization Rate
Aerobic
Anaerobic
1 1.83 1.81
2 1.79 2.68
3 2.45 7.34
4 2.73 7.36
5 2.54 4.17
6 2.60 3.00
7 3.99 NA
8 3.79 NA
9 7.57 NA
10 2.64 NA
11 2.39 NA
12 2.00 NA
15 1.64 NA
18 1.81 NA
24 1.81 NA
'- (percent biodegradation/elapsec time in days) x 100
`
Table 5
Soil Viability by Glucose Mineralization: Inceptisol
Month
Glucose Mineralization Rate1
Aerobic
Anaerobic
1 2.48 1.95
2 4.15 3.89
3 8.60 8.44
4 8.90 7.76
5 8.29 3.34
6 8.97 3.02
7 9.50 NA
8 9.47 NA
9
10.46
NA
10 8.91 NA
11 8.50 NA
12 8.04 NA
15 8.14 NA
18 8.06 NA
24 8.90 NA
(percent biodegradation/elapsec time in days) x 100
p. 40
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Table 6
Soil Viability by Glucose Mineralization: Ultisol
Month
Glucose Mineralization Rate1
Aerobic
Anaerobic
1 2.23 2.22 2 2.56 2.39 3 1.67 2.86 4 1.95 4.22 5 4.04 5.41 6 2.46 3.80 7 3.79 NA 8 7.33 NA 9 7.37 NA 10 8.83 NA 11 2.99 NA
12 3.61 NA
15 8.19 NA
18 2.37 NA
24 3.65 NA
'- (percent biodegradation/elapsed time in days) x 100
Table 7
Soil Viability by Glucose Mineralization: Mollisol
Month
Glucose Mineralization Rate1
Aerobic
Anaerobic
1 2.36 1.85
2 2.54 2.99
3 1.72 2.57
4 3.63 3.99
5 4.06 4.82
6 3.66 4.16
7 3.74 NA
8 3.95 NA 9 3.60 NA
10 7.51 NA
11 2.73 NA
12 1.78 NA
15 3.59 NA
18 2.11 NA
24 1.81 NA
*- (percent biodegradation/elapsed time in days) x 100
p. 41
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Table 8 Soil Microbial Biomass (pg/g) (ISO 14240-2; fumigation-extraction)
ALFISOL
INCEPTISOL
_____Aerobic____________ Anaerobic_____
Aerobic
Anaerobic
Treated Untreated Treated Untreated______ Treated Untreated Treated Untreated
Post Inc.
-
244
-
128
Month 4 317 323 435 287
Month 6 680 279 593 645
Month 12 769 675 NA NA
- 131 - 132 138 109 184 106 432 483 423 467 504 564 NA NA
p. 42
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Table 8 (continued)
Soil Microbial Biomass (pg/g) (ISO 14240-2; fumigation-extraction)
Post, Inc. Month 4 Month 6 Month 12
MOLLISOL Aerobic ________Anaerobic_____ Treated Untreated Treated Untreated
- 190 - 53
216 218 218 205
243 259 267 270
714 734 NA NA
ULTISOL
______ Aerobic____________ Anaerobic Treated Untreated Treated Untreated
- 312 -
54
36 31 26 34
486 194 500 407
565 570 NA NA
NA - Not analyzed
p. 43
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-42Table 9 Typical GC/MS Operational Parameters for Analysis of 8-2 FTOH in Headspace Gas
Instrument:
Detector: Analytical Column:
Injector Temperature: Oven Temperature Profile:
Hewlett-Packard Model 5890 Series II Gas Chromatograph (GC)
Hewlett-Packard Model 5971 Mass Selective Detector
Phenomenex Zebron ZB-5 (30 m x 0.25 mm, 0.25-pm film thickness).
150C Initial temperature: 50C Initial hold time: 2.00 minute
Ramo:
Rate CC/minutei 20.00 50.00
Final Temoerature CCI 100 300
Detector Temperature: Carrier Gas: Septum Purge: Split Vent: Injection Volume: Data Acquisition Mode:
Final hold time: 3.00 minute 280C Helium, ~1 mL/min; CHP = ~14 psi Helium, ~5 mL/min ~40 mL/min 2 pL (2 stops w/ 10 pL syringe), splitless SIM
Acquisition Ions:
Component 8-2 FTOH
Quantitation 31 m/z
Confirmation 95 m/z
Peak Retention Times:
HDFD
33 m/z
98 m/z
8-2 FTOH: Approximately 5.0 minutes
HDFD: Approximately 5.0 minutes
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Table 10
Typical LC/MS/MS Operational Parameters for Analysis of 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in Soil Extracts
Instrument:
Analytical Column: Column Temperature: Mobile Phase:
Gradient Elution Profile:
Injection Volume: Monitored Ion Transitions:
PFOA 2-13 PFOA (internal standard) 8-2 FTA 8-2 FTUA PFNA PFDA PFUA LC/MS/MS Analog Parameters:
Agilent Technologies 1100 Series High Performance Liquid Chromatograph with an Applied Biosystems/MDS SCIEX API 3000 LC Mass Spectrometer equipped with a Perkin-Elmer Turbo IonSpray source. Operated in the negative ion multiple reaction-monitoring mode.
Zorbax RX-C8 (150 mm x 2.1 mm, 3-pm particle size)
40C A: 0.1% Formic Acid in Reagent Water
B: Acetonitrile
Time /mint
0.00 1.00 8.00 9.00 9.10 14.00 15.00 20.00
Flow Rate /uL/minl
250 250 250 250 250 250 250 250
Percent A
70.0 70.0 15.0 15.0 5.0 5.0 70.0 70.0
Percent B
30.0 30.0 85.0 85.0 95.0 95.0 30.0 30.0
50.0 pL
Ql
413.0 amu 415.0 amu 477.0 amu 457.0 amu 463.0 amu 513.0 amu 563.0 amu
Q3
369.0 amu 370.0 amu 393.0 amu 393.0 amu 419.0 amu 469.0 amu 519.0 amu
Dwell Time
250 msec 250 msec 250 msec 250 msec 250 msec 250 msec 250 msec
Retention Time
9.82 min 9.82 min 12.2 min 12.3 min 10.6 min 11.2 min 11.8 min
IS =-4500 TEM = 400 DP = -10 FP = -140 EP = -10
CE = -15 CXP = -25 CUR = 8 CAD = 4 NEB = 12
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-44Table 11 Typical LC/MS/MS Operational Parameters for Analysis of 8-2 FTOH in Soil Extracts
Instrument:
Analytical Column: Guard Column: Column Temperature: Mobile Phase:
Isocratic Profile:
Injection Volume: Monitored Ion Transitions:
8-2 FTOH HDFD (internal standard) LC/MS/MS Analog Parameters:
Agilent Technologies 1100 Series High Performance Liquid Chromatograph with an Applied Biosystems/MDS SCIEX API 3000 LC Mass Spectrometer equipped with a Perkin-Elmer Turbo IonSpray source. Operated in the negative ion multiple reaction-monitoring mode.
THERMO EC Betasil C-18 (50 mm x 2.1 mm, 5-pm particle size) Javelin Betasil C-18 (20 mm x 2.1 mm)
40C
A: 0.15% Acetic Acid in Reagent Water B: Acetonitrile
Time (min')
0.00 6-00
Flow Rate CuL/mm)
250 250
Percent A Percent B
30.0 70.0 30.0 70.0
25.0 pL
Q1
523.1 amu 528.1 amu
IS = -4500 TEM = 450 DP = -25 FP = -250 EP = -10
Q3
58.9 amu 58.9 amu
Dwell Time
500 msec 500 msec
Retention Time
4.18 min 4.15 min
CE = -50 CXP = -10 NEB = 12 CUR = 7 CAD = 6
p. 46
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-45 Table 12 Typical LC/MS/MS Operational Parameters for Analysis of 7-3 Acid and 7-2 sFTOH in Soil Extracts
Instrument:
Analytical Column: Column Temperature: Mobile Phase:
Gradient Elution Profile:
Injection Volume: Reported Ion Transitions:
7-3 Acid 2-13 PFOA (internal standard) 7-2 sFTOH HDFD (internal standard) LC/MS/MS Analog Parameters:
Agilent Technologies 1100 Series High Performance Liquid Chromatograph with an Applied Biosystems/MDS SCIEX API 3000 LC Mass Spectrometer equipped with a Perkin-Elmer Turbo IonSpray source. Operated in the negative ion multiple reaction-monitoring mode.
Zorbax RX-C8 (150 mm x 2.1 mm, 3-pm particle size)
40C
A: 0.15% Acetic Acid in Reagent Water
B: Acetonitrile/ 0.15% Acetic Acid
Time ("min)
0.00 1.00 1.01 7.00 12.00 13.00 20.00
Flow Rate (uL/min)
250 250 250 250 250 250 250
Percent A Percent B
95.0 5.0 95.0 50.0 70.0 30.0 15.0 85.0 15.0 85.0 95.0 5.0 95.0 5.0
50.0 pL
Q1 441.0 amu 415.0 amu 473.0 amu 528.0 amu
Q3
337.0 amu 370.0 amu 59.0 amu 59.0 amu
Dwell Time
50 msec 50 msec 50 msec 50 msec
Retention Time
11.6 min 10.0 min 11.9 min 12.1 min
IS = -4500 TEM = 400 EP = -10 CUR = 7 CAD = 6 NEB = 132
7-3 Acid/ 2-13 PFOA DP = -40 FP = -350 CE = -15 CXP = -11
7-2 sFTOH/ HDFD -25 -250 -50 -10
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Project No.: 112E-108
Method flowchart for the analysis of 8-2 FTOH in the headspace of soil test chambers
For each sample, fit a C]8 cartridge between the end of an appropriate volume syringe and a hypodermic needle.
I
With the plunger fully depressed, insert the needle of the disposable syringe through the septum of the sample and pull a full syringe volume. Remove needle, expel headspace air and repeat.
i Detach C18 cartridge and place in appropriate holder. Attach the barrel of a 10 mL disposable syringe to Cj8 cartridge and use a volumetric pipette to transfer acetonitrile extraction solvent into the syringe barrel. Insert the syringe plunger into the barrel and inject the acetonitrile through the C,8cartridge into a 20-cc glass scintillation vial. Fortify the extract to contain 100 ng/mL ID, ID, 2D, 2D, 3-13C Heptadecafluoro
Decanol internal standard.
I
Pipette an aliquot of the extract directly into an autosampler vial. Cap vial and submit for GC/MS analysis of 8-2 FTOH. All sample vials not analyzed immediately are place in refrigerated storage.
I Store the remaining trap extract in scintillation vials under frozen conditions.
Figure 1. Method flowchart for the analysis of 8-2 FTOH in the headspace o f soil test chambers.
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p. 48
Projectno.:iheios
Method flowchart for the analysis of 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in soil extracts
For each sample vessel, remove crimp seal and place foil liner into the vessel. Using a graduated cylinder, measure and transfer 125 milliliters of extraction solvent into each sample vessel. The extraction solvent consists of 100 ng/mL ID, ID, 2D, 2D, 3-13C Heptadecafluoro Decanol internal
standard in acetonitrile. Add a 10-mL aliquot of 200 mM NaOH into each sample.
4
Re-crimp each sample vessel with a new aluminum crimp cap and the original septum. Extract the samples for approximately three hours using a gyratory shaker table set for 250 rpm.
4
Allow suspended material in extracts to settle. Remove a 10-mL aliquot of the extract using a serological pipette and transfer into a separate 50-mL plastic centrifuge tube preloaded with approximately 25 mg of Envi-CarbTM. Centrifuge samples at a setting of 12000 rpm for 10 minutes. Transfer extract supernatant for each sample into a separate labeled scintillation vial.
4
For 8-2 FTOH analyses, pipette an aliquot o f the extract supernatant (or diluted extract, if applicable) with reagent water fortified with 100 ng/mL ID, ID, 2D, 2D, 3-13C Heptadecafluoro Decanol (HDFD) internal standard to produce a final solution composition of 1:1 (v/v) extract: water. For 7-2 sFTOH
analyses, pipette an aliquot of the extract supernatant (or diluted extract, if applicable) with reagent water fortified with 100 ng/mL HDFD to produce a final solution composition of 1:3 (v/v) extractiwater.
Perform dilution directly in an autosampler vial, cap and vortex. Submit for HPLC/MS/MS analysis of telomer alcohols. All sample vials not analyzed immediately, place in refrigerated storage.
4
For PFCA analyses of each sample, dilute an appropriate volume o f the extract supernatant (or diluted extract, if applicable) with reagent water fortified with 1.00 ng/mL N-Perfluorooctanoic Acid, [1,2 di13C] internal standard to produce a final solution composition of 1:3 extract: water. Perform dilution
directly in an autosampler vial, cap and vortex. Submit for HPLC/MS/MS analysis o f PFCAs. All sample vials not analyzed immediately, place in refrigerated storage.
4
Re-crimp each sample vessel with a new aluminum crimp cap and the original septum. Store sample vessels and extract scintillation vials under frozen conditions.
Figure 2. Method flowchart for the analysis of 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS in soil extracts.
p. 49
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Wildlife International, Ltd. GC-MS#2 MSD Serial No. 3118A02483
File: Operator:
T:\DATA\ALFI025\0101005.D DW
Date Acquired: 20 Apr 106 5:41 pm
Instrument: GCMS#1
Sample Name:
TA-30
Misc Info:
25.0 ug a.i./L
Vial Number : 1
Figure 3.
Representative ion chromatograms for 8-2 FTOH in a low-level (25.0 ng a.i./mL) calibration standard by GC/MS.
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Wildlife International, Ltd. GC-MS#2 MSD Serial No. 3118A02483
File:
T:\DATA\ALFI025\0501009.D
Operator:
DW
Date Acquired: 20 Apr 106 7:03 pm
Instrument: GCMS#1
Sample Name:
TA-34
Misc Info:
500 ug a.i./L
Vial Number : 5
Figure 4. Representative ion chromatograms for 8-2 FTOH in a high-level (500 ng a.i./mL) calibration standard by GC/MS.
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Project No.: 112E-108
Figure 5 Representative ion chromatogram for 8-2 FTOH in a low-level (10.0 ng a.i./mL) calibration standard by LC/MS/MS.
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Project No.: 112E-108
Figure 6.
Representative ion chromatogram for 8-2 FTOH in a high-level (1000 ng a.i./mL) calibration standard by LC/MS/MS.
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Project No.: 112E-108
Figure 7.
Representative ion chromatograms for 7-2 sFTOH in a low-level (5.00 ng a.i./mL) calibration standard by LC/MS/MS.
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Project No.: 112E-108
Figure 8. Representative ion chromatograms for 7-2 sFTOH in a high-level (250 ng a.i./mL) calibration standard by LC/MS/MS.
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Project No.: 112E-108
Figure 9. Representative ion chromatogram for 8-2 FTA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.
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I W.FWWtWl"fto k Nun* *6-2(5)' **<**) *47709a0i ` c ---.....Cenm* *1MMT Amdrtiov
S e ttle Indax:
33
S a ^ la Type:
Standard
C a c u m cio n :
5 .0 0 ug
C a lc u lacad Cone: 4 . 1 5 0 ug
BunchiI ' 'Mediti ad;
Er o e. A lg o ritb i sin g Pacte) e Thru*hol e
a Threshold
AdJ. I
MrTOWLMBJl
3Dad* 29*422*42J*42a425a*-
23*422a*-
project no.: 112E-108
MIB.O
8009. 70OE. 600.
30CD.
200.
Figure 10. Representative ion chromatogram for 8-2 FTA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.
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Project No.: 112E-108
Figure 11. Representative ion chromatogram for 7-3 Acid in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.
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Project No.: 112E-108
Figure 12. Representative ion chromatogram for 7-3 Acid in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.
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-58-
Project No.: 112E-108
Figure 13. Representative ion chromatogram for 8-2 FTUA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd
-59-
Project No.: 112E-108
Figure 14. Representative ion chromatogram for 8-2 FTUA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
,W ildlife International, Ltd
-60-
Project No.: 112E-108
Figure 15. Representative ion chromatogram for PFOA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd,
-61 -
ProjectNo.: 112E-108
Figure 16. Representative ion chromatogram for PFOA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
,W ildlife International, Ltd
I s*npwNm "ample.WT" 'UampielD 'ILiUMuB'l' hh M i m M 1 lbULAfcir
Peek Name X-SAdd" Mu*(as)'`463A '4190 nir
"
Cerniw r *LCMS-1S' A rroaton:-
Sattpl Index:
1C
ic p le Type:
Standard
Concent r a t l i n :
0.100 ug a . i . / L
C a lc u la te d Cer.e: O.OSOS1C ug a . i . / k g
ic q . Cate:
0 4 /2 1 /2 0 0
K edltled: F re e . Alge I Bunching Fi
He
-62-
Project No.: 112E-108
Figure 17. Representative ion chromatogram for PFNA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd
-63 -
Project No.: 112E-108
Figure 18. Representative ion chromatogram for PFNA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-64-
Project No.: 112E-108
Figure 19. Representative ion chromatogram for PFDA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI
W ildlife International, Ltd
-65 -
Project No.: 112E-108
Figure 20. Representative ion chromatogram for PFDA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
.W ildlife International, Ltd
-66-
Comma TCMS-18' Anrowen
aiapla Inda.-.:
in
S a a p lt Types
Standard
Is d itle d .
N:
Pros. Algorithm: Analyst C
T h raa h eld : 25.41
3 8*4 36*4 3 4M 3.2M 30M
2 4M 2.2M
Project No.: 112E-108
12M
8000.0 8000 0 4000.0 2000.0-
o.o-L
i
9 10 11
Figure 21. Representative ion chromatogram for PFUA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd
-67-
Project No.: 112E-108
Figure 22. Representative ion chromatogram for PFUA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-68 -
p. 69 Project No.: 112E-108
IS Adjusted Peak Area Counts
Concentration (fig 8-2 FTOH/L) Figure 23. Representative calibration curve for 8-2 FTOH by GC/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd
-69-
Project No.: 112E-108
Figure 24. Representative calibration curve for 8-2 FTOH by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
.W ildlife International, Ltd
-70-
Project No.: 112E-108
Figure 25. Representative calibration curve for 7-2 sFTOH by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd
-71 -
Project No.: 112E-108
A nalv te A r e a / IS A re a
Figure 26. Representative calibration curve for 8-2 FTA by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-72-
Project No.: 112E-108
Figure 27. Representative calibration curve for 7-3 Acid by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd
-73 -
Project No.: 112E-108
A na l v t e A r e a / IS A re a
Figure 28. Representative calibration curve for 8-2 FTUA by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-74-
Project No.: 112E-108
042006_M12_TELALAE.ndb (PFOA C-8): "Linear" Regression ("1 / x" weighting): y = 1.12 x + 0.0538 (r = 0.9985)
X n a l v t e A rea / IS A re a
1-5 2.0 ' 2.5 ' ' 3C ' ' 315 ' ' 40 ' 4 .5 ' ' ' 5.0 ' 5 5 ' ' 6.'o
_____________________ Analyte Cone. / IS Cone.
Figure 29. Representative calibration curve for PFOA by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd
-75 -
Project No.: 112E-108
An alvte A re a / IS A re a
042006_M12_TELALAE.rdb (C-9 Add): "Linear" Regression {"1 / x" weighting): y = 1.34 x + -0.00636 (r = 0.9991)
9.0 8.5 8.0 7.5 7.0 6.5 6.0 5.5 5.0 4.5 4.0 3.5 3.0 2.5 2.0 1.5 1.0 0.5
0.0
0.5 1.0 1.5 2 0 2.5 3.0 3.5 4.0 4.5 5.0 5 5 6.0 6.5 Analyte Cone. / IS Cone.__________________________ ______
Figure 30. Representative calibration curve for PFNA by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
Wildlife International, Ltd.
-76-
Project No.: 112E-108
An alvte A re a / IS A re a
042006_M12_TELALAE.rdb (C-10 Acid): `lin e a r Regression ("1 /x" weighting): y= 1.4 x + -0.0404 (r = 0.9996)
9.5 9.0 8.5 8.0 7.5 7.0 6.5 6.0 5.5 5.0 4.5 4.0 3.5 3.0 2.5 2.0 1.5 1.0 0.5,
o.oK
0.5 1.0 1 5
2.0 2 5 3.0
3.5 4.0
______ Analyte Cone. / IS Cone.
4.5
5.0 ' 5 5
:
6.'o' ' ' 6 5 `
Figure 31. Representative calibration curve for PFDA by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd
-77-
Project No.: 112E-108
042006_M12_TELALAErdb(C-11 Acid): "Lineai' Regressen ("1 /x" weighting): y= 1.29 x + 0.0743 (r = 0.9994)
A n a lv te A r e a / IS A re a
Figure 32. Representative calibration curve for PFUA by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
Project No.: 112E-108
-78-
Wildlife International, Ltd. GC-MS#2 MSD Serial No. 3118A02483
File:
Operator:
C:\HPCHEM\l\DATA\ALFI005\0701011.D
fj1
Date Acquired: 30 Apr 105 4:10 pm
Instrument: GCMS#2
Sample Name: Misc Info:
1570-TEX
Vial Number : 7
Figure 33. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C,gcartridge extract) from a Day 0 untreated control soil by GC/MS.
8-2 FTOH was not present (no peak at 5.0 min).
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-79-
Project No.: 112E-108
Figure 34. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 untreated control soil by LC/MS/MS.
8-2 FTOH was not present (no peak at 4.18 min).
Company Sanitized. Does not contain TSCA CBI.
Wildlife International, Ltd.
-80-
Project No.: 112E-108
Figure 35. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Day 0 untreated control soil by LC/MS/MS.
7-2 sFTOH was not present (no peak at 11.9 min).
Company Sanitized. Does not contain TSCA CBI
W ildlife International, Ltd.
-81 -
Project n o .: i u e - ios
r ^u U S S S im
DOF Samps ID '15717 Ffa.*04210$_DQ_TELALAE.<>
PMk Nam: *9-2 (Sr Maee<ee). `477.OW3.0 wmt`
S a a pClea n Innadnet x":ll2E-10S.11D4 #.H 0-(UTCr A n w w e o n -
S aaple Type: C e n cen tiitica:
B lank 0.00 ug a .i ./ L
1 00*4
C a lc u la te d Cone:
N/A
Acq. Date:
0 4 /2 1 /0 5 0 1 : 10:04 PM
9600.00
nodi tied : Bunching fa c to r: N oise T hreshold: A rea T hreshold:
Nun. Smooths : RT window:
t x p e c t e d RT: Sep. W idth:
Sep. H eight: Exp. Peek R atio
Exp. A di R atio Exp. v a l. R atio
U se R e la tiv RT
No
2 4 .0 6 120.21
3 0 .0 12.2 0 .2 0 0 .0 1 5 .0 0 4 .0 0 3 .0 0 No
cps epa
sun
9000 00 9500.00 9000 00 7500.00 700000
I n t . Type:
A rea: H eight: S t a r t Tiise: End T ine:
No Peak
N/A N/A
N/A N/A
in eps
sun
4600 00 9000 00
$900000 I!
li|
2500.00 2000.00 1500.00 1000.00
900 00
I: 000
Printing Date: Friday, April 22,2005
9.04
\
10.74
__
I W . 7JOT3 8.13 10 39 ' A , . . . .
____ _ . ____ _
T 9'7 ~
-r-
^ T3 * 15 16 17 IS IS
__
_ _Ta.in
. . . __________________________________________ _
Figure 36. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 untreated control soil by LC/MS/MS.
8-2 FTA was not present (no peak at 12.2 min).
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-82-
Project No.: 112E-108
Figure 37. Representative ion chromatogram for 7-3 Acid in the soil extract from a Day 0 untreated control soil by LC/MS/MS.
7-3 Acid was not present (no peak at 11.6 min).
Company Sanitized. Does not contain TSCA CBI
W ildlife International, Ltd
*S*mpta_006- $*m p4 ID ' T O * Fie: TO 105_D0jr6LA LA E wr
PM k Mem*. *B-J (U r
*4X7.0003 0 amu*
Commant *11 -IO 0.100.1 ICH^TC)* Ann t o r **
sa ap l* Index: S u p U Type: : C oncentration: : C a lc u la te d Cone:
i Acq. D ote: | Acq. T ine:
14 B lank
0 .0 0 N/A W /21 /0 5
0 3 :1 0 :3 4
u a .i./L PM
1 00*5 B 50*4
| M odified: , Bunching F acto r: N o is e T h r e s h o ld : 1A re T h re sh o ld ;
1Mu*. Sm ooths: ] k t Window:
: E x p e c te d RT: .S e p . W idth: [ Sep. H eight: 1Exp. Peak R eno | Exp. A d). R atio
; Exp. V al. Raeic ( U se R e l a t i v e P.T
Ho
2 0 1 .9 3 1 0 0 9 .6 4
3 0 .C 1 2 .3 0 .2 0 0 .0 1 5 .0 0 4 .0 0 3 .0 0 No
epa eps
see m in
900*4 1 50*4 6.00*4 790*4 7 00*4
! In t. Type: i R e t e n t i o n T1m :
A rea: {H eight; , S ta r t Ti m : i End Ti m :
No Peak N/A
N/A N/A
N/A N/A
eps m in tain
6 60*4
6 00*4
5:
j $.00*4
Ii
S 4.50*4
4 00*4
3.60*4
3.00*4
2.60*4
200*4
-83 -
Project No.: 112E-108
1 000 '
Printing Date: Friday, April 22,2005
i '3
____________ __ 604 a.fr! _7.w SJS 10.16 11,10 12.67-, 1 3 .4 2 ,13.05 >
_ _________ ^
3 4 51
T ' V ~
10 11 12 13 14 1 1 I ?
_________________________ T o r*, non______________________________________
__ _____
Figure 38. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0
untreated control soil by LC/MS/MS.
.
8-2 FTUA was not present (no peak at 12.3 min).
Company Sanitized. Does not contain TSCA CBI.
.W ildlife International, Ltd
[| S*mp>* N * w : -&mp>*_00r SanvN IO : ^5 7 tT "H a: 'M 2 ia 5 '"Do' TELALAE.wHT
|| PM k Name: *PFOA C -T
'413.04309.0 Knu*
""
j C i r n w n t `t12E-108.10e,11CKVTCr Annotation: "
i s u f i Indue: i!i S a ha fpll* T iympae
14 fBt l al annl ik
I C o n centration:
c .o o ug a . i . /L
1.00*5 ,
j C a lc u la te d Cone:
KVA
' Acq. D ate: Acq. T in e:
04/21/0S 0 7 :1 0 :0 4 PM
9.50*4
; M odified: ! Bunching f a c to r :
I N oise T hresh o ld : ' A rea T hreshold: : Nun. Saooths: | NT Window: . E x p e c te d NT: 1 Sep. W idth:
Sep. H eight: Exp. Peak R atio: Exp. Ad}, ftacio: Exp. V al. R atio: Use R e l a t i v e RT:
No 1 1 8 3 .7 7
9 4 8 .6 4 1
3 0 .0
9 .8 2 0 .2 0 0 .0 1 5 .0 0 4 .0 0 3 .0 0 No
cps cps
sec in
9.00*4 8.50*4 ! 8X0*4 j 7X0*4 7.00*4 ^
In t. Type:
R eten tio n T iae: A rea: N eighs: S ta rt T iae: End T l*K :
No P eak
H/A N/A N /A
N/A N /A
ln i
c o u n ts cps
in in
6.50*4
j
.00*4 |
}
'S !j- 4X0*4
4.00*43.50*4 3.00*4 2.50*4 2.00*4
-84-
5000.00! 000
Project No.: 112E-108
Figure 39. Representative ion chromatogram for PFOA in the soil extract from a Day 0 untreated control soil by LC/MS/MS.
PFOA was not present (no peak at 9.82 min).
Company Sanitized. Does not contain TSCA CBI
W ildlife International, Ltd
Project No.: 112E-108
-85 -
71TS S5:
S rtl.l0 .-t5 7 0 - H >: -l2iui.00.TE LLE .
li PM k N am : *C-e M f Me*<*>: -63.0M1BJD imu" I C nm w c*1l2E -t0e.1(.11H W T Cr AnnoWion."
i! S u ^ l i I n d e x : ` S a w p le T y p e: il C o n e e n t r e t i e r :
C alc u lated Cone:
14 81*k
0 .0 0 ug
N/A
a .i
1.00*5 .
0 50*4 :
K o dified: Bunching F a c to r:
N ois* T h resh o ld : A re* T h resh o ld : Hu m . S a o o t h i :
RT w indow :
E x p ec te d RT: Sep. W idth: Sep. H eight:
Exp. Peak R a tio : Exp. Ad}. R atio : Exp. V al. R atio:
U se R e la tiv e RT:
NO l
1 9 2 .8 5 9 6 4 .2 5
1 3 0 .0
1 0 .6
0 .2 0
0 .0 1 5 .0 0 4 .0 0
1 .0 0
Ho
: T o t. Type: R e te n tio n Tit**:
1 A rea: , H eight:
| S ta r t Ti m : ! End Ti m :
HO P eak N/A
M/A N/A
M/A N/A
cps cps sec w in
w in c o u n ts cps
w in
9.00*4 8.50*4 8.00*4 750*4 7.00*4 6.30*4 6.00*4
5 5**
5 4.50*4-
4 00*4
3 50*4 3.00*4
2.50*4
1 50*4 1.00*4 3000.00
OOO
Printing Date: Friday, April 22,2005
5~SL. 3 * . , f i* 5.83!-. 66-.0W1 -A*3>4 7.66 3 l 0 . 2 > J f l I t 1250 1353
Figure 40. Representative ion chromatogram for PFNA in the soil extract from a Day 0 untreated control soil by LC/MS/MS.
PFNA was not present (no peak at 10.6 min)
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-86-
Project No.: 112E-108
I SewpieMem* *Srapl*..gOr Semple 10'`l&fT >4* "042105'DO 'TELALRE.wtfT
I. PeetNeme-C-IOAdd- U w |n ) -S13.0a8.0amu*
"~
j Comment*112E>108.10B,110-<inCr AnnokSbon "
. Sample Index:
14
Sample Typ:
Blank
C oncentration:
0.00. 1191.1./L
100*5.
- C a lc u la te d Cone: n/ a
Aeq. D ate: Acq. T lx:
04/21/05 0 1 :1 0 :0 4 PH
9.90*4
M odified: - bunching F acto r: j N oise T hreshold: ! A res Threshold: 11 Pint, sm o o th s:
!| RT Window: ': E x p ec te d ST: I Sep. W idth: : Sep. Heighc: . Cxp. Peak R atio:
Exp. Ad). R atio: Exp. V al. R atio: Use R e la tiv e RT:
Ho J
167.30
SS e.S O l
3 0 .0 11.2 C.20 c.01 S.00 4 .0 0
3 .0 0 No
cps cps
sec min
9.00*4 8.50*4 8.00*4 7.50*4 7.00*4
i, l n t . T ype: R e te n tio n Time:
A rea: l| H e ig h t:
; S tart T iw : End Time:
Me Peak n /a
n/A N/A
N/A N/A
6J0*4 ] 6.00*4 ;
5, 1 I6.00*4 <
4SOM )
4.00*4-1
350*4 !
250*4 j 2.00*4
1.00*4 500000 i 000
Printing Date: Friday, April 22,2005
_2.08 3.18 .SJ3.4J56.85 8.01 AS7 7.77.7J7 0.81 X>M 1 1 2 2 ,11.80 13.a0.13J*
___
2*
48
8 7 6 5 10 11 72 15 14 IS 18
IT ' 18 " '
Figure 41. Representative ion chromatogram for PFDA in the soil extract from a Day 0 untreated control soil by LC/MS/MS.
PFDA was not present (no peak at 11.2 min).
Company Sanitized. Does not contain TSCA CBI
W ildlife International, Ltd
-87-
I--.^ 7 = 7
- -- T * 1" CTTr Fie W2l0S_00_TELALA6tr
PeekNeme: *C-11 Aenf
*5 0 0018.0 am*
I S n pCl et nInmd l x*:112E-1C0.140.t10-<UTCr
S a ^ le Typ:
Blank
Cooc e n t r t io n :
0 .0 0 ug
C a lc u la te d Cor
H/A
Acq. Dite:
04/21/05
I Acq. Ti m :
0 7 :1 0 :0 4 PK
A m M ten** 00a5 |
JON j
< M ediiied: i. Bunching f a c t o r : !i H ois* T h re sh o ld :
I' Area T h re s h o ld :
'< mm. S a e o th s : j RT Window:
expected kt:
9ep. W idth: Sep. H eight: Exp. peak R a tio :
Exp. Ad3 . R a tio :
;! Exp. V a l. R a tio : ! Ose R e la tiv e RT
Mo 1 114.03
570.16
1 3 0 .0
u .<
0.20 C.01 5 .0 0
4 .0 0
3.00
DON SOn ]
Xr.t. T ype:
He Peak
Retention T ine:
H/A
SOee
H eight: S tart T iae: End T ia e :
!i
H/A
H/A H/A
l
Project No.: 112E-108
,00e4
2.00* i
1.D0H !
i
900000 : 000 - --
Printing Date: Friday, April 22,2005
~4 S ! 7 837 8.01 BJ1 730 0.70 40
.si. m i lc e is a o n jB
10 "TT 12 14 is" 1 17 10 10
Figure 42. Representative ion chromatogram for PFUA in the soil extract from a Day 0 untreated control soil by LC/MS/MS.
PFUA was not present (no peak at 11.8 min).
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-88-
Project No.: 112E-108
W i l d l i f e I n t e r n a t i o n a l , L t d . Q C ^M S#2 M S I) S e r i a l No;.
F I Xe ;
p e r tea ts
X.:/\CMST\ 1 \m T A \A I -HZ;4- 1 :Vt>.,61010 . 0
o ft
....
.
b $ t e A c q u ir e d : I S A> 103f 3:B pari
In s tru m e n t:
tSBM'S#! '
"
Sam ple Name:
15S2VTEJX:
M ia c ln e .i
V ia l Num ber : 6
''
3 .1 1 S A 0 2 4 S 3
Figure 43. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C18cartridge extract) from a Month 24 untreated control soil by GC/MS.
8-2 FTOH was not present (no peak at 5.0 min).
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-89-
Project No.: 112E-108
Figure 44. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 24 untreated control soil by LC/MS/MS.
8-2 FTOH was not present (no peak at 4.18 min).
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-90-
Project No.: 112E-108
Figure 45. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Month 24 untreated control soil by LC/MS/MS.
7-2 sFTOH was not present (no peak at 11.9 min).
Company Sanitized. Does not contain TSCA CBi.
W ildlife International, Ltd.
-91 -
Project No.: 112E-108
Figure 46. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 24 untreated control soil by LC/MS/MS.
Trace 8-2 FTA at 12.3 min was <LOQ.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-92-
Project No.: 112E-108
Figure 47. Representative ion chromatogram for 7-3 Acid in the soil extract from a Month 24 untreated control soil by LC/MS/MS.
7-3 Acid was not present (no peak at 11.6 min).
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-93 -
Project No.: 112E-108
Figure 48. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 24 untreated control soil by LC/MS/MS.
8-2 FTUA was not present (no peak at 12.3 min).
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-94-
Project No.: 112E-108
Figure 49. Representative ion chromatogram for PFOA in the soil extract from a Month 24 untreated control soil by LC/MS/MS.
Trace PFOA at 9.91 min was <LOQ.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-95 -
Project No.: 112E-108
Figure 50. Representative ion chromatogram for PFNA in the soil extract from a Month 24 untreated control soil by LC/MS/MS.
Trace PFNA at 10.7 min was <LOQ.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-96-
Project No.: 112E-108
Figure 51. Representative ion chromatogram for PFDA in the soil extract from a Month 24 untreated control soil by LC/MS/MS.
Trace PFDA at 11.4 min was <LOQ.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-97-
Project No.: 112E-108
Figure 52. Representative ion chromatogram for PFUA in the soil extract from a Month 24 untreated control soil by LC/MS/MS.
Trace PFUA at 12.0 min was <LOQ.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
Project No.: 112E-108
-98-
Wildlife International, Ltd. GC-MS#2 MSD Serial No. 3118A02483
File:
C:\HPCHEM\1\DATA\ALFI005\0801012.D
Operator:
fjl
Date Acquired: 30 Apr 105
Instrument: GCMS#2
Sample Name:
4:29 pm 1719-TEX
Mise Info: Vial Number : 8
Figure 53. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Ci8 cartridge extract) from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil
by GC/MS.
-
8-2 FTOH was not present (no peak at 5.0 min).
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-99-
p. 100 Project No.: 112E-108
Figure 54. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.
p 101
Company Sanitized. Does not contain TSCA CBI
W ildlife International, Ltd.
-100-
00T Semple *D: *17W Fie 'O4210S_D0.TELALAE.wifr
Peek Heme *a-2<Sr Mb u (m ):'477.0093.0 amu*
CowoL-ttJ6-l06,10e.110-(SPtKEr Aiw wMon"
i. S a l a l e In d e x : Saeple Typ:
IS
i CC oa nl ccuelna ttreadc i Cono :r .c : 7 .7 S 1 9 ug *
Acq. D ate: Aeq. Tim e:
0 4 /2 1 /OS 0 7 :3 1 :2 7 PH
M odified: I Bunching r a c to r :
B oise T hreshold: | A rea T h resh o ld :
Bus. Smooths: XT W indow:
| E x p e c te d XT: i; S e p . W id th :
T Sep. H eight: Exp. Peak R a tio :
Exp. Adj . R a tio : ;> E x p . v a l . R a t i o : > Use R e la tiv e RT:
No 1
2 4 .0 6 1 2 0 .3 1
1 3 0 .C
1 2 .2 0 .2 0
0 .0 1 S.OO
4 .0 0 3 .0 0 He
9000 00 8500 CO 9000 00 7600.00 7000 00
I n t. Type:
R e te n tio n T isie: A rea: H eight:
S t a r t Ti m
Base To Base 12.2 ain
1S075. counts 2931.21603 epa
1 2 .4
4000.00 3500DO -
ii 3000-00 Ii i1
j| 2500.00-I : 2000.00 \
1500.00 ; 1000.00 !
12.23
0 X ---------- . ------- --
0 * 702 5 .X 6.7B 10.06,
Project No.: 112E-108
Figure 55
Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 fortified (250 jig 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-101 -
Project No.: 112E-108
Figure 56
Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS
p. 103
Company Sanitized. Does not contain TSCA CBI
W ildlife International, Ltd.
Project No.: 112E-108
- 102-
I Sampte N am : 5emp<*_OOB* i ^ t o l D i - l f i r Fite: *042106J30_TEtALeiWr P w k Heme *PFOA C-F Uaaa4*s) *413 0068.0 eexT C m iM it *112E-108.l08.110-(SPIKEr Annotetav"
Sample Index:
IS
Sample T ype: C oncentration;
c= 1 0 .0
1.00*5
C a lc u la te d Cone: 9.1702 uq a .i ./ k q
Aeq. D ate: Acq. Time:
0 4 /2 1 /0 5 . 0 7 :3 1 :2 7 PM
8.50*4
| M odified: ' Punching F a c to r: M oise T h re sh o ld :
' A rea T hreshold:
: Hum. S m o o th s: ! RT Window: ! E x p ec te d RT:
Sep. w idth:
1Sep. H eight:
! Exp. Peak R atio: 1 Exp. A d] R a tio : i Exp. V al. R a tio : 1 Use R e la tiv e RT:
No 1 189.77 948.84 1 3 0 .0 9 .8 2 0 .2 0 0 .0 1 5 .0 0
3 .0 0 NO
<=P epa
sec m in
8.00*4 8 50*4 8 .0 0 t 7 50*4 700*4
! In t. Type:
Pase To E tse
j! R e te n tio n T im e :
9 .8 1 min
!! A re :
170080. counts
l H e i g h t:
28273.54469 epa
! S t e r t Time:
9 .8 7 min
End T im e:
1 0 .0 min
860*4 j )
6.00*4 j
I :.. 5.50*4 |
^ 5.00*4 j
4.00*4 3.30*4
i
3.00*4 2J0M 2.00*4 1J0*4 1.00*4
5000.00 0.00 -
8.62
3 4 r 7 8 8 10 ___________________ Time, r
12 13 14 15 ' 18 17* 18
Figure 57.
Representative ion chromatogram for PFOA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
,W ildlife International, Ltd
- 103 -
Project No.: 112E-108
Figure 58.
Representative ion chromatogram for PFNA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 104-
p 105 Project No.: 112E-108
Figure 59.
Representative ion chromatogram for PFDA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-105 -
I Sam Niw: 'wipf_00r 6anli 1D.`1>ir F4*. *042105 00 TELALAE.Mfr~
Peek Name *C-11 K f *<): *5*3.0/519.0 mu'
~~
Cwiwwnc*112E-10e.10e.110K8PIKr AmoMon:*-
:: S a e p le I n d e x :
IS
S tap le Type: C oncentration:
QC 10.0 Uf a .i ./ L
*"11 OQeS-j
!| C a lc u la t e d C one: 9 .1 8 9 4 u g a . l . / k g
i; AAceqq.. B a te :
0047:/2311/:0257 W
*.S0e4-
Bunching T ac to r: Noi T h resh o ld : i Ar T h resh o ld : Hun. Sneoth: AT Window: E x p e c te d AT:
S e p . width.: | Sep. H eight: | Exp. Peak R atio: ! Exp. A d). R atio:
Exp. V al. R atio: ; O R e la tiv e RT:
1 114.03 5 7 0 .1 6
1 3 0 .0
1 1 .0
0 .2 0 0 .0 ] 5 .0 0 4 .0 0
3 .0 0 No
ep Bin
in t. Type: R etention Tu A rea: i H eight : ' S t a r t Tiate:
End T in e :
Ease To Base
1 1 .8 Bin 198870. count
32316.82269 cps 11.6 Bin
1 2 .0 Bin
0.00*4 ' 8 50*4 8.00*4 760*47.00*4 j
t
^ 5.00*4
p. 106 Project No.: 112E-108
2 00*4 ]
5000 00000
11.81
t.OI 031 7 08 7 0 9 3 5 0.79 11.5 7 . , ^ *123913.91 .1306 7 8 10 11 12 13 14 15 18 17 " ie
19
Figure 60.
Representative ion chromatogram for PFUA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
p. 107 Project No.: 112E-108
- 106-
Wildlife International, Ltd. GC-MS#2 MSD Serial No. 3118A02483
File:
T:\DATA\ALFI025\0801012.D
Operator:
DW
Date Acquired: 20 Apr 106 8:04 pm
Instrument: GCMS#1
Sample Name:
1735-TEX
Misc Info:
112E-108,109,110
Vial Number : 8
Figure 61. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Cjs cartridge extract) from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by GC/MS.
8-2 FTOH was not present (no peak at 5.0 min).
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 107-
p. 108 Project No.: 112E-108
Figure 62. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.
Trace 8-2 FTOH at 3.98 min was <LOQ.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-108-
p. 109 Project No.: 112E-108
Figure 63.
Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI
W ildlife International, Ltd.
- 109 -
p. 110 Project No.: 112E-108
Figure 64.
Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
.W ildlife International, Ltd
- 110-
p. 111 Project No.: 112E-108
Figure 65.
Representative ion chromatogram for PFOA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.
. Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- Ill -
p. 112 Project No.: 112E-108
Figure 66.
Representative ion chromatogram for PFNA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 112-
p. 113 Project No.: 112E-108
Figure 67.
Representative ion chromatogram for PFDA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 113 -
p. 114 Project No.: 112E-108
Figure 68. .
Representative ion chromatogram for PFUA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.
p. 115
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
Project No.: 112E-108
- 114-
Wildlife International, Ltd. GC-MS#2 MSD Serial No. 3118A02483
File:
C:\HPCHEM\1 \DATA\ALFI 005\1201020.D
Operator:
fj 1
Date Acquired; 30 Apr 105
Instrument: GCMS#2
7:04 pm
Sample Name:
1665-TEX
Misc Info: Vial Number : 12
Figure 69. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Cj8cartridge
extract) from a Day 0 sterile soil treated with 200 mg/kg(_
}by GC/MS.
8-2 FTOH was not present (no peak at 5.0 min).
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 115 -
p. 116 Project No.: 112E-108
Figure 70. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 sterile
soil treated with 200 mg/kgQ
J b y LC/MS/MS.
Trace 8-2 FTOH at 4.09 min was <LOD.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 116-
I SajrgXe'Nsir. " en(ijj14- SamplelO l e e r ' Fie `042105 DCTEtALAE.wWr
Peak Mane: *9-2 i r Meea1ee}.*477.Qra930em'
~"
Conenent *112E-106-* Awtetnon."
Seaple Index:
20
Seaple Type:
unknown
C o n c en tra tio n :
W/A
IJW
C eleuleted Cone: 0.0000 ug e .i./k g
, Aeq. Date:
04/23/0$
. Acq. Tu n :
09:18:31
PR00.00
Bunching T a s te r :
H eist Threshold: Area Threshold: Hu. Sm ooths:
' ST Window: S sp ected RT:
, Sep. Width: ' Sep. H eight:
Lxp. Teak R a tio : Exp. Adj. R atio : Exp. V al. R atio : Use R e la tiv e RT:
120.31
30.0
0.01 5.00 4.00 3.00
ops sec
9000.00 9500.00 9000.00 7900.X } 7Q00.X j
I n t. Type: R ese ctio n Tia Area:
H eight: S t a r t Time:
Sne Tine:
Ho Peak
OSCO-
I:
00.001
p. 117 Project No.: 112E-108
2000.00 1S00.M
m oo 04 : T25
Printing Date: Friday, A p ril 22 ,2 0 0 5
. il
10.99 ^ y S g
__ _
_
S 9 7 9 9 10 ' 11 12 13 14 IS 19 17 ' 1* ' 19
Figure 71.
Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 sterile
soil treated with 200 mg/kg
by LC/MS/MS.
8-2 FTA was not present (no peak at 12.2 min).
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
p. 118 Project No.: 112E-108
- 117-
r i f ' Ssmpis Nam: S#mpi*_CH' Sampto 1C 'I M S ' Fie *042106jW.JteukLAESSr*
;[ P M k N*m*- *9-2 < u r Mee*<**) '457 00 9 3 .0 emu*
: H Carrwnanc *112E-1C9-* A nnonaon **
S a n p lt Index:
20
S eaple Type:
Unknown
C on cen tratio n :
H/A
1.00*5 -
C a lc u la te d Cone: 0.0000 ug a . i . / k q
Acq. D ate: A cq. Tim e:
0 4 /2 1 /0 5 0 9 : 1 8 :3 1 PH
950*4
!i M o d if ie d : ! B u n c h in g r e c t o r : |! N o is e T h r e s h o l d : : A re a T h r e s h o l d :
jl Nu b . S m o o th s : l! AT W indow : ' E x p e c te d RT: ' Sep. W idth:
Sep. H eight: Exp. Peak A atio : Exp. A dj. R atio : >j E x p . V a l . R a t i o : ij U se R e l a t i v e RT:
No 1 2 0 1 .9 3 1 0 0 9 .6 4
1 3 0 .0
1 2 .3 0 .2 0 0 .0 1 S.OO 4 .0 0 3 .0 0 No
cps cps
sec Bin
900s4 4.50*4 4.00*4 7.50e4 7.00*4 -
ij X n t. T y p e : R e te n tio n T ine:
| A rea:
1 H e i g h t : 1 S t a r t T i e :
i| End T iB e :
No P e s l N/A
N/A
N/A N/A
N/A
Bin c o u n ts cps
Bin
Bin
4.50*4
9.00*4-
5
5.00*4 : j
4.50*4 -
3.50*4 j
3.00*4 I
I2.50*4 -j
2.00*4-j
1.50*4 j
i
aoo Printing Date: Friday, April 22,2005
23
75 iF" "i77-S? 812 9.79 10.12 116 n J6 13-33. 13-93
6 7 9 9 "tO 11 12 13 14
19
___Time, min____________
_
Figure 72.
Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0
sterile soil treated with 200 mg/kg
by LC/MS/MS.
8-2 FTUA was not present (no peak at 12.3 min).
Company Sanitized. Does not contain TSCA CBI
,W ildlife International Ltd.
- 118-
p. 119 Project No.: 112E-108
Figure 73.
Representative ion chromatogram for PFOA in the soil extract from a Day 0 sterile
soil treated with 200 mg/kg(_
"]by LC/MS/MS.
Trace PFOA at 9.81 min was <LOQ.
Company Sanitized. Does not contain TSCA CBI.
,W ildlife International Ltd.
p. 120 Project No.: 112E-108
- 119-
SampaNam* *Sm/iY SamphTS^fSr 'V ii' -042105 DOTEl AEw
Peak Nam* 'C 4 Add* hha^aa): `4630M15 0a> `
"" ~
Comment *1T2-tC6-` Aiwotaton ~
Saaple In d ex :
20
s u p l t Type:
Unknown
C oncentration:
N/A
1 00*5
C a lc u la te d Core: 0.0000 ug 4 . 1 . /k g
Acg. D ate: Acq. Ti m :
0 4 /2 1 /0 5 09:18:31 m
9.50*4
M odified: Aunehing P a sto r:
N oise Thxcataold: Area T h resh o ld :
N w . Sm ooths: IT Window: E x p e c te d RT:
Sep. W idth:
Sap. H eight: Exp. Peak R atio: la p . Ad}. R atio :
Exp. V al. R atio: Uae R e la tiv e IT :
19 2 .e` 9 6 4 .2S
3 0 .0 10.6 0 .2 0 0 .0 1 5 .0 0 4 .0 0 3 .0 0 No
cpi cps
9 00*4 8.50*4 8.00*4 7 50*4 7.00*4
R eten tio n Ti m :
H eight: S t a r t Ti m : End T ina:
No Peak
N/A H/A
N/A N/A
in
epa in in
e 30*4 8 00*4
5
500*4
' OOM 4A M
3.50*4
3 00*4
250*4 -
2AM 1 50*4 :
1 00*4
500000-
Printing Date: Friday, Aprii 22,2005
12 a
_ S*
. ,___
iD - O j0;8* . 0.82 13 0 , 13.72____ ____________ ______ _____
f 5 * 1 11 12 13 M*" 16 17 11 i l
Figure 74.
Representative ion chromatogram for PFNA in the soil extract from a Day 0 sterile
soil treated with 200 mg/kgC
Jby LC/MS/MS.
PFNA was not present (no peak at 10.6 min).
p. 121
Company Sanitized. Does not contain TSCA CBl
W ildlife International, Ltd.
Project No.: 112E-108
-120-
T SenvieNaine'^Senptt OW Sampiell Reek Not : *C-10Ac*r Meet*) -513.O46S.0 *< Comment '112E-106-" AmoMen-**
Sawpi* In d ex :
20
S n p t t T y p e: Unknown
C o n c en tra tio n :
M/A
C a lc u lated Cone: 0.35761 ug * . : . / k g
I Acq. S a t :
04/21/0$
Acq. T ia e :
0 9 :1 8 :3 1 PM
M odified: Punching Facto r: Sola T hreshold: Area T hreshold:
Ituw. Sw ooths: RT Window: E x p ected RT:
Sap. w idth: Sep. H eight: Exp. Peak P atio
Exp. A d;. P atio
Exp. V al. R atio
Use R e la tiv e RT
167.30 3 6 .$ 0
3 0 .0 11.2 0.20 0.01 $.00 4.00 3.00 No
cps cps
sec win
9.00e4 ; a.S0e4 |
I7.50*4 J
7 00*4 |
K i t To Rase
R eten tio n Ti m :
1 1 .2 win
A re a:
7192.7 counts
H eight:
1226.09099 cps
S ta rt Tine:
11.1 win
End Time:
11.3 win
0.50*4 j
0.00*4 1
t
5
j j 5.00*4
" 490*4
4 00*4
3 90*4 :
2.00*4 | 1.60*4 |
' 1.00*4 * ! sooo o o ' I ___ :i
Printing Date: Friday, April 22,2005
2 ' *" 4
___________ __________ 57 00
" a 3 1230 13 > 3__________
. _______
10 11 12 13 14 15 16 17 10 9
Figure 75.
Representative ion chromatogram for PFDA in the soil extract from a Day 0 sterile
soil treated with 200 mg/kg^
^ p y LC/MS/MS.
Trace PFDA at 11.2 min was <LOQ.
Company Sanitized. Does not contain TSCA CBI
,W ildlife International Ltd.
p. 122 Project No.: 112E-108
- 121 -
1''~ S am j * & m '* 3 M * 3 t 014* "simpW O:*1085* F: *042109 DO T D oAESt ' F**fc Nam*' `C-11 Aa f Ma--( . . ) *553.0610.0 amu* S a a pClaemImnadnrx*1: 136-10*-2*0 AnneMan-**
Saapl* Typ:
Unknown
C oncentration:
X/A
00*5
C a l c u l a t e d C e n e: 0 .0 8 2 9 4 0 U9 a . i . / k q
Acq. D ata: Acq. T i*e:
0 4 /2 1 /0 5 0 9 :1 8 :3 1 PH
1.90*4
M odiliaC : :: B u n c h in g F a c t o r : [ N o ia* T h r e s h o ld :
Ara* T h resh o ld : Ik a. Soootha: ' RT Window:
E x p ec te d RT:
Sep. W idth: 5p. H eight:
Exp. Peak R a tio : . E x p . AO3. R a tio :
Exp. V al. R a tio : ' Uaa R e l a t i v e RT:
Ko
114.03 S I C .15
1 3 C .0
1 1 .8 C . 20 0.01 5 .0 0 4 .0 0 3 .0 0 Mo
cps epa
sac M in
I n t. Type: l a t a n t i m Tiata:
H eight: ' S ta rt T iae: . End Ti *m :
Rasa To Baa* 11.1 Min
35*70.4 c o u n ts 5 : . l l t 4 t epa
1 1 .7 Min
12.0 Min
9.00*4 -
8 50*4-'
900*4 1
i
7.50*4 !
! 9 50*4 i
8.00*4
5.00*4-
4-50*4;
4.00*4
I3.60*4 4 300d4J
! 50*4
200*4
1.50*4 100*4
OOO
Printing Date: Friday, April 22,2005
Ma a nsa. *r40>
1262 13.42-1 3 J3
Figure 76.
Representative ion chromatogram for PFUA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg "]by LC/MS/MS.
Trace PFUA at 11.8 min was <LOD.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
p. 123 Project No.: 112E-108
-122 -
Wildlife International, Ltd. GC-MS#2 MSD Serial No. 3118A02483
File:
T:\DATA\ALFI025\1301021.D
Operator:
DW
Date Acquired: 20 Apr 10S 11:09 pm
Instrument: GCMS#1
Sample Name:
1682-TEX
Misc Info:
112E-108
Vial Number : 13
Figure 77. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C]8 cartridge
extract) from a Month 12 sterile soil treated with 200 mg/kgC
Jby GC/MS.
Company Sanitized. Does not contain TSCA CBI
W ildlife International, Ltd.
- 123 -
p. 124 Project No.: 112E-108
Figure 78. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 12 sterile soil treated with 200 mg/kgC 7 by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI
W ildlife International, Ltd.
- 124-
p. 125 Project No.: 112E-108
Figure 79.
Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg_ 3 by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd
- 125 -
Project No.: 112E-108
Figure 80.
Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 12
sterile soil treated with 200 mg/kg
Jby LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 126-
p. 127 Project No.: 112E-108
Figure 81.
Representative ion chromatogram for PFOA in the soil extract from a Month 12
sterile soil treated with 200 mg/kg__
")oy LC/MS/MS.
Company Sanitized. Does not contain TSCA CBl.
W ildlife International, Ltd.
- 127-
p. 128 Project No.: 112E-108
Figure 82.
Representative ion chromatogram for PFNA in the soil extract from a Month 12
sterile soil treated with 200 mg/kg^
by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI
.W ildlife International, Ltd
-128-
p. 129 Project No.: 112E-108
Figure 83.
Representative ion chromatogram for PFDA in the soil extract from a Month 12 sterile soil treated with 200 mg/kgC Jb y LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd
-129-
Project No.: 112E-108
Figure 84.
JRepresentative ion chromatogram for PFUA in the soil extract from a Month 12
sterile soil treated with 200 mg/kg
by LC/MS/MS.
p. 131
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
Project No.: 112E-108
- 130-
Wildlife International, Ltd. GC-MS#2 MSD Serial No. 3118A02483
File:
C:\HPCHEM\1\DATA\ALFI005\1001014.D
Operator:
fjl
Date Acquired: 30 Apr 105 5:06 pm
Instrument: GCMS#2
Sample Name:
1593-TEX
Misc Info:
'
Vial Number : 10
Figure 85. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Ci8cartridge extract) from a Day 0 live soil treated with 200 mg/kg(_ ^ b y GC/MS.
8-2 FTOH is not present (no peak at 5.0 min).
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-131 -
p. 132 Project No.: 112E-108
Figure 86. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 live soil treated with 200 m g / k g Q b y LC/MS/MS.
Trace 8-2 FTOH at 3.93 min was <LOD.
Company Sanitized. Does not contain TSCA CBI.
Wildlife International, Ltd.
-132-
p. 133 Project No.: 112E-108
Figure 87. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Day 0 live soil
treated with 200 mg/kgjT
Joy LC/MS/MS.
Trace 7-2 sFTOH at 11.9 min was <LOQ.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
I Sample Name:'Sampi* Oil* Sample ID:'1563* FI 'O42U_D0_TElALA T Peek to rn * l - J (S r U n t i l ) ' **77.00830 emu* Comment *112E-105-"
j S u p l i Index:
17
S aaple Type:
Unkn
C oncentration:
H/A
| Calculate! Cone: < 0
I Aeq. Dace:
0 4 /2 1 /0 5
* A cq. Ti m :
05:14:1 I
M odified: : B u n c h in g f a c t o r ::
N oiaa T h re s h o ld :: i< A re a T h r e s h o ld : ij Mum. Sm o o th s: I- RT Window:
E x p e c te d BT: , Sep. W idth: jj S e p . H e i g h t : | E xp. Pea): R a tio : ij E x p . A d}. R a tio : '! E xp. v a l . R a tio : ' Use R e l a t i v e RT:
NO 1
2 4 .0 6
120.31
1 3 0 .0
1 2 .2 0 .2 0 0.01
5 .0 0 4 .0 0 3 .0 0
No
cps cps
sec m in
8000 00
50000 000.00
7500.00 '
i
7000.00 I
In t. Type:
. R e te n tio n Ti m : A rea: H eight: S ta r t Tuee:
End Time:
Base To Base
12.2 Bin 73.81 count! 142.09353 epa
12.1 n in 12.4 Bin
5600.00 |
5000.00
,
j j 600000 |
~ *50000
4000.00
3600.00*
3000.00 ]
jj
1500.00' 100000 500.00
- 133 -
p. 134 Project No.: 112E-108
Figure 88. '
Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 live
soil treated with 200 mg/kg(j
^by LC/MS/MS.
Trace 8-2 FTA at 12.2 min was <LOD.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 134-
p. 135 Project No.: 112E-108
Figure 89.
Representative ion chromatogram for 7-3 Acid in the soil extract from a Day 0 live soil treated with 200 mg/kg' JJby LC/MS/MS.
7-3 Acid is not present (no peak at 11.6 min).
Company Sanitized. Does not contain TSCA CBI.
W ildlife InternationalLtd.
p. 136 Project No.: 112E-108
- 135 -
SainptaNama: "Sampt# O'M* SwnptotO:`iSo* Fii.;tM2106.00_TELALAE wrfr
Pn K Hans: *6-2(Uf ktte#(a*).*437.0383.0 i W
Cemmant *112E-106-* Annotaten "
j] S a a v l* I n d e x :
17
|] S u p l i T y p e:
Unknown
j! C o n c e n t r a tio n :
N/A
j C a lc u la te d Cone: 0.0000
ug a .i./k g
I COOS'
|i A cq . D o t e j A eg . Ti m :
04/21/05 0 0 :1 4 :1 PH
8.30*4 -j
M odified: Bunching F a c to r:
'N o ra* T h resh o ld : X rtt T hreshold:
Num. S tao o th e : | AT Window:
E x p e c te d AT:
Sep. W idth: Sep. H eight: Exp. Peak R atio : Exp. A d). R atio: Exp. vl. R atio : U se R e l a t i v e AT:
No 1*
2 0 1 .9 3 1009.64
1 3 0 .0 1 2 .3
0 .2 0 0.01 S .0 0 4 .0 0 3 .0 0 Ko
cps cp*
sec n in
9 00*4 -j 30*4 2
730*4 7.00*4
In t. Type: R e te n tio n Titae: A rea: H eight: S t a r t Ti m : End T in e :
No Peak N/A
n/ a N/A
n/A N/A
Bin c o u n ts cp* m in n in
6.30*4
I.
^ 5.00*4 ,
3 50*4 |
3.00*4 j
2.50*4 [
I
2.00*4 |
1.00*4 I
j500000
0.00 - ..................... ..... 23
4 ~5
B7
*9 10 11 12 13 14 15 1 ta. win
Figure 90.
Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 live
soil treated with 200 mg/kg((
Jby LC/MS/MS.
8-2 FTUA was not present (no peak at 12.3 min).
Company Sanitized. Does not contain TSCA CBI
W ildlife International, Ltd.
- 136-
I Sempts Herrn:$*B*l*_0ir Sanpt* ID;'1583' Me: TM210SJ P**k NtttM- *PFOA C-T M**<#*): *413.0/389.0 amu*
CammanC ` 1121-1 Oft-' A nnoM en:-
S ama ppll*e TI nydpe x :
Un1k7nown
C oncentration:
N/A
C a lc u la te d Cone: C.6S38S uq a . i . / k q
Acq. D ate: Acq. Tim*:
0 1 /2 1 /0 5 0 8 :1 4 :1 8 PH
0*4 <
M odified:
Eunehinq F a c to r: N oise T h resh o ld :
A rea T hresh o ld : Mum. S m o o th s: FT Window:
E x p e c te d RT:
Sep. W idth:
Sep. H eiqht: Exp. Peek R atio Exp. AdJ. R atio Exp. VaL. R a tio Use R e l a t i v e RT
1 8 9 .7 7 9 4 8 .8 4
3 0 .0 9 .8 2 0 .2 0 0 .0 1 5 .0 0 4 .0 0 3 .0 0 No
epa CP.
sec in
9.00*4 ' 6.S0*4 8.00*4 ' 7.50*4 7.00*4
In c. Type: R e te n tio n Time: A rea: H eight: S t a r t Time: End Time:
Rase To la se 9 .8 3 min
14347. 2460.59297 cps
9 .7 0 1 0 .0
6.50*4
600*4
fr. 5.90*4
5O0M
* 4.50*4-.
i 4.00*4 -
i j 3.50*4
3.00*4
2.50*4
2.00*4
1 S04
5000.00 ; 0.00 `
5070
p. 137 Project No.: 112E-108
12 13 14 15 18 17 18 IS
Figure 91.
Representative ion chromatogram for PFOA in the soil extract from a Day 0 live soil
treated with 200 m g/kgf
_^by LC/MS/MS.
Trace PFOA at 9.83 min was <LOQ.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-137-
Ttempi* Nam*- `tempi P n k Nam*. "C-B A otf Comment *112E-tOB-'
Oil* Sample ID.*1593* F4*.*04210S_00_TEUMA.w M*M(**): '483.0M19.0aimT
Sam ple In d e x :
1?
S n p lt Type:
Unknown
C oncentration:
N/A
C a lc u la te d Cone: 0.J792S
Aeq. D ata:
0 1 /2 1 /0 !
Acq. Tim e:
08:11:11
j M odified: : Bunching F a c to r:
N oise T h resh o ld : A rea T h resh o ld : Mum. S m o o th s: RT W indow : E x p e c te d RT: Sep. W idth: 1 S e p . H e i g h t: >; e x p . P eak R a t i o : 'i E x p . A d } . R a t i o :
-J E x p . V a l . R a t i o : U se R e l a t i v e RT:
No 1 1 9 2 .8 5 6 4 .2 5
3 0 .0 10.6 0.20 0.01 5 .0 0 4 .0 0 3 .0 0 No
900*4 J
In t. Type: R etention ` A rea: , H e i g h t: : S ta r t Tu n : \ End Time:
E ase To Base 1 0 .6 min
5541.0 c o u n ts 984.41642 cps
1 0 .4 a m 1 0 .7 a in
p. 138 Project No.: 112E-108
3.50*4 j 3.00*4 ] 2.50*4 ! 2.00*4-
5000.00 000
Figure 92.
Representative ion chromatoeram for PFNA in the soil extract from a Day 0 live soil treated with 200 mg/kg _J b y LC/MS/MS.
Trace PFNA at 10.6 min was <LOD.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-138-
MtU.tM_TKLALAE.Si
p. 139 Project No.: 112E-108
li KT Hindovi
1tspaettd AT: ||*p. Widch: || S*p. Migtic:
30.0 11-2 0 .2 0 *0.01
liSup. Ad}. >*: .00 ,!Cap. Vj, A*tlc: 3.00
in
Jteitn tio n Tli Art*: . tight:
i7.90*4 i 7.00*4
f
130* ' 3.00*4 1JM 1.00*4 9000.00
4& I7 09
*14 IS " 17 ' 19 S '
Figure 93.
Representative ion chromatogram for PFDA in the soil extract from a Day 0 live soil treated with 200 mg/kg_ J b y LC/MS/MS.
Trace PFDA at 11.2 min was <LOQ.
Company Sanitized. Does not contain TSCA CBI,
W ildlife International, Ltd.
p. 140 Project No.: 112E-108
- 139-
I SampI* Nam:-SengM* S ii* Pa k Nam' "C-1 f Adid"
S * n * tetO .`1S03* FU*: "042106 DO TELALAEwtr *903 0 5 1 0 .0 amu*
t a a pCiocrmImndnetx"1: 12E-106-*17 AnneM tc n ~
Saaple Type: C o n centration:
Unknown N/A
1 OOaS-
C a lc u lated Cone: 0.0000 ug a .i./k g
Acq. D ate: Acq. T ine:
0 4 /2 1 /0 5 0 8 :1 4 :1 8 PK
9.50e4
. M odified: bunching f a c to r :
N oise T h resh o ld : A rea T h resh o ld : Nun. Sm ooths: ! RT Window: E xpected RT:
Sap. W idth:
Sep. H eight: Bxp. Peak R a tio : Bxp. A dj. R a tio : Bxp. V al. R a tio : Use A a l a t i v a RT:
Mo
1 1 1 4 .0 3 5 1 0 .IS
1 3 0 .0
1 1 .8 0 .2 0
0 .0 1 5 .0 0 4 .0 0 3 .0 0 Ho
eps cps
sec sun
S.00e4 6.50a4 6.00*4 7.50*4 7.00*4
X nt. Type:
! R e te n tio n Tim e: A rea:
! h eig h t:
: S t a r t Tima: ' End T i m :
No Peak V/A
N/A N/A
N/A K/A
m id cps
m in
6.50*4 6.004
j, S 4.50*4 j
ti I 4 004 -
S .0 M 2.50*4 2.00*4 1.50*4 1.00*4 600000 j
0.00 ^ -------- ---------- ---------- ----------,---- 12 3 4 5
--------------------- , - , ------ 1--------- ,----------r ------ --------------------- -----------,--------, ------------------ -- 6 7 5 9 10 11 12 13 14 15 16 17 16 19
. Tima,win
____________________________
Figure 94.
Representative ion chromatogram for PFUA in the soil extract from a Day 0 live soil treated with 200 mg/kg_ ^ by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
.W ildlife International, Ltd
- 140-
p. 141 Project No.: 112E-108
Figure 95. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Cis cartridge
extract) from a Month 24 live soil treated with 200 mg/kg(j
Jb y GC/MS.
Company Sanitized. Does not contain TSCA CBI.
.W ildlife International, L td
- 141 -
p. 142 Project No.: 112E-108
Figure 96. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 24 live
soil treated with 200 mg/kgt
J b y LC/MS/MS.
Trace 8-2 FTOH at 3.96 min was <LOQ.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 142-
p. 143 Project No.: 112E-108
Figure 97. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Month 24 live
soil treated with 200 mg/kg
by LC/MS/MS.
Trace 7-2 sFTOH at 11.9 min was <LOQ.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 143 -
p. 144 Project No.: 112E-108
Figure 98. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 24 live
soil treated with 200 mg/kg
Jby LC/MS/MS.
Trace 8-2 FTA at 12.3 min was <LOQ.
Company Sanitized. Does not contain TSCA CBI
W ildlife International, Ltd.
- 144-
p. 145 Project No.: 112E-108
Figure 99. Representative ion chromatogram for 7-3 Acid in the soil extract from a Month 24 live
soil treated with 200 mg/kg
J b y LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 145 -
p. 146 Project No.: 112E-108
Figure 100. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 24 live
soil treated with 200 mg/k^Q
^ by LC/MS/MS.
Trace 8-2 FTUA at 12.3 min was <LOQ.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 146-
p. 147 Project No.: 112E-108
Figure 101. Representative ion chromatogram for PFOA in the soil extract from a Month 24 live soil
treated with 200 mg/kg
_Jby LC/MS/MS.
Note:
Secondary dilution required and reported for PFOA. Original analysis was over calibration range.
Company Sanitized. Does not contain TSCA CBI
W ildlife International, Ltd.
- 147-
p. 148 Project No.: 112E-108
Figure 102. Representative ion chromatoeram for PFNA in the soil extract from a Month 24 live soil
treated with 200 mg/kgC
T3by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 148-
p. 149 Project No.: 112E-108
Figure 103. Representative ion chromatogram for PFDA in the soil extract from a Month 24 live soil
treated with 200 mg/kgC
J y LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI
W ildlife International, Ltd.
- 149-
p. 150 Project No.: 112E-108
Figure 104. Representative ion chromatogram for PFUA in the soil extract from a Month 24 live soil
treated with 200 mg/kg
]by LC/MS/MS.
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
p. 151 Project No.: 112E-108
- 150 Appendix I Measured Concentration of Selected Analytes (pg/kg Soil Dry Weight)
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-151 -
p. 152 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFOA (pg/kg)
in Soils Treated with f
3
Soil Type Ultisol
Sample Interval Day 0 Weekl Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
Aerobic Live Sterile
<LOQ <LOQ
17.8 19.1 32.0 36.5 57.8 61.4 122.1 150.0 269.7 373.8 376.4 441.1 487.0 558.6 367.7 344.1 340.1 362.6 410.7 328.7 365.9 329.1
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
2.0 <LOQ
2.4 <LOQ
2.2 <LOQ
4.9 <LOQ
7.8 <LOQ <LOQ <LOQ
Anaerobic Live Sterile
<LOQ <LOQ
21.7 22.7 26.8 23.9 51.5 40.3 70.0 73.3 114.7 133.5 148.6 104.7
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
2.5 <LOQ
2.8 <LOQ
LOD = limit o f detection (0.206 pg/kg) LOQ = limit o f quantitation (2.0 |ig/kg)
Thursday, N ovem ber 08, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-152-
p. 153 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFOA (jig/kg)
in Soils Treated with
J
Sample Soil Type________ Interval
Mollisol
DayO
Week 1
Week 2
Week4
Month 2
Month 4
Month 6
Month 9
Month 12
Month 15
Month 18
Month 24
Aerobic Live Sterile
<LOQ <LOQ
15.4 15.1 17.8 18.0 21.7 20.2 31.8 30.3 46.0 58.6 74.7 74.4 92.5 69.3 103.8 94.0 98.5 247.9 126.9 118.8 229.3 207.5
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
2.9 <LOQ
3.2 2.3 3.4 3.3 3.0 3.1
LOD = limit of detection (0.206 pg/kg) LOQ = limit of quantitation (2.0 pg/kg)
Anaerobic Live Sterile
<LOQ <LOQ
14.7 15.1 18.8 18.1 20.2 22.3 42.8 43.2 78.5 61.5 117.4 91.0 .
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
2.7 <LOQ
3.6 2.4
Thursday, N ovem ber OS, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 153 -
p. 154 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFOA in Soils Treated with
Soil Type
Sample Interval
Aerobic Live Sterile
Alfisoi
--
DayO Week 1 Week2 Week4 . Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24
<LOQ <LOQ
8.5 8.2 11.4 11.0 14.9 15.8 23.2 25.0 32.0 33.1 58.4 52.5 97.9 93.7 65.4 84.2 133.1 103.5 194.4 155.9 196.5 190,7
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
3.3 <LOQ <LOQ <LOQ
LOD = limit o f detection (0.206 pg/kg) LOQ = limit of quantitation (2.0 pg/kg)
Anaerobic Live Sterile
<LOQ <LOQ
9.4 10.9 14.1 15.4 19.7 19.8 17.9 31.1 90.0 77.8 99,3 83.5
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
2.0 <LOQ
2.2 <LOQ
Friday, O ctober 22, 2Q7
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 154-
p. 155 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFOA (jrg/kg)
in Soils Treated with
^
Soil Type Inceotisol
1Sample Interval . Day 0 Week 1 Week 2 Week 4 Month2 Month 4 Months Month 9 Month 12 Month 15 Month 18 Month 24
Aerobic
Anaerobic
Live Sterile______ Live Sterile
<LOQ <LOQ
18.9 20.8 28.6 29.0 55.7 54.7 122.2 97.7 190.4 189.4 264.7 250.4 288.5 339.5 367.8 350.0 402.3 385.3 422.5 446.5 530.9 554.1
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
2.1 <LOQ
2.8 <LOQ
4.4 <LOQ
5.6 3.0 6.2 2.8 4.2 2.5
<LOQ <LOQ
20.9 17.7 26.5 26.8 75.5 48.1 138.0 144.3 284.5 302.6 327.0 347.1
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
5.0 <LOQ
7.6 <LOQ
LOD = limit of detection (0.206 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg)
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-155 -
p. 156 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 8-2 FTUA Qig/kg) in Soils Treated w ith ^ 3
Sample
Aerobic
Anaerobic
Soil Type________ Interval____________Live Sterile______ Live Sterile
Ultisol .
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24
<LOD <LOQ <LOQ <LOQ
2.1 2.5 4.0 3.2 6.3 6.7 12.3 12.7 12.1 19.3 30.9 19.7 6.0 7.6 11.3 13.5 15.6 6.4 15.6 32.3
<LOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD
CLOD CLOD CLOD CLOD <LOQ cLOQ <LOQ CLOQ CLOQ CLOQ cLOQ cLOQ cLOQ CLOQ
cLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD
LOD = limit o f detection (0.119 pg/kg) LOQ = limit of quantitation (2.0 pg/kg)
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------j ---------------------------------------------------------------
-156-
p. 157 Project No.: 112E-108
Wildlife International, Ltd,
Project Number 112E-108
Measured Concentrations of 8-2 FTUA (fig/kg)
in Soils Treated w ith
^
Soil Type Mollisol
Sample Interval Day 0 Week 1 Week2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24
Aerobic Live Sterile
CLOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ cLOQ cLOQ CLOQ CLOQ CLOD cLOQ CLOQ cLOQ <LOQ cLOQ CLOD CLOD CLOQ CLOQ
3.0
cLOQ
CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOQ CLOQ CLOD CLOD
LOD = limit o f detection (0.119 pg/kg) LOQ = limit o f quantitation (2.0 ig/kg)
Anaerobic Live Sterile
cL O Q CLOQ CLOQ cLOQ CLOD CLOD CLOD cLOQ cLOQ CLOQ CLOQ CLOQ cLOQ CLOQ
CLOD CLOD cLOQ cLOD CLOD CLOD cLOD CLOD CLOD cLOD CLOD CLOQ CLOD CLOD
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 157-
p. 158 Project No.: 112E-108
Wildlife International, Ltd,
Project Number 112E-108
Measured Concentrations of 8-2 FTUA (fig/kg)
in Soils Treated w ith^
~2
Soil Type Alfisol
Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
Aerobic Live Sterile
<LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOD <LOD <LOD <LOD -CLOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ
Anaerobic Live Sterile
<LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOQ
3.9 2.2 2.3 <LOQ
<LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ
LOD = limit o f detection (0.119 gg/kg) LOQ = limit o f quantitation (2.0 gg/kg)
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBi.
W ildlife International, Ltd.
- 158-
p. 159 Project No.-: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 8-2 FTUA (fig/kg) in Soils Treated with (f
Soil Type Incentisol
Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
Aerobic Live Sterile
<LOQ <LOQ <LOD <LOD <LOQ <LOQ
5.9 5.0 25.2 12.3 77.7 88.9 164.0 160.4 192.1 161.0 209.5 206.9 225.6 '25C2'i.11 256.2 242.7 248.5 239.0
<LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD
2.7 <LOQ
2.2 <LOQ <LOQ <LOQ
Anaerobic Live Sterile
<LOD <LOD <LOD <LOD <LOD <LOQ
7.7 <LOQ
5.6 5.5 13.0 13.0 15.4 10.0
<LOD CLOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ
LOD = limit o f detection (0.119 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg)
Friday, October 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-159-
p. 160 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 8-2 FTA (fig/kg)
in Soils Treated with.
^
S oil Type Ultisol
Sam ple In terval Day 0 W eek 1 W eek 2 W eek 4 M onth 2 M onth 4 M onth 6 M onth 9 M o n th 12 M o n th 15 M o n th 18 M onth 24
A erobic L ive S terile
<LOD <LOD <LOQ <LOQ
3.8 3.7 6.2 4.7 8.6 8.5 9.7 8.0 10.1 19 0 19.7 12.0 3.0 4.4 4.3 3.8 6.7 2.9 4.7 5.2
<LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD
2.0
A naerobic L ive S terile
<LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD
<LOD <LOD <LOD <LOD <LOD <LOD <LOD -CLOD <LOD CLOD <LOD -CLOD <LOD <LOD
LOD = limit of detection (0.503 pg/kg) LOQ = limit of quantitation (2.0 pg/kg)
F riday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 160-
p. 161 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 11ZE-108
Measured Concentrations of 8-2 FTA (pg/kg) in Soils Treated withC
Soil Type Mollisol
Sample Interval DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
Aerobic U v e Sterile
cLOD <LOQ <LOQ <LOD <LOD CLOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ
2.3 <LOD <LOD <LOD <LOD <LOQ CLOQ
<LOD <LOD <LOD <LOD <LOD <LOD CLOD cL O D CLOD CLOD CLOD cL O D CLOD cLOD CLOD CLOD CLOD cLOD
LOD = limit of detection (0.503 pg/kg) LOQ = limit of quantitation (2.0 pg/kg)
Anaerobic Live Sterile
CLOD CLOD CLOD <LOD CLOD CLOD <LOD cLOD cLOD cL O D cL O D cLOD CLOD CLOD
CLOD cLOD CLOD cL O D CLOD cL O D CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD
Friday, October 12 ,2 0 0 7
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 161 -
p. 162 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 8-2 FTA (jig/kg) in Soils Treated with
Soil Type Alfisol
Sam ple Interval Day 0 Week 1 Week 2
Week 4
Month 2 Month 4
Month 6
Month 9
Month 12
Month 15 Month IS Month 24
Aerobic Live Sterile
<LOD <LOD <LOD <LOD <LOD <LOD <L0D <LOD <LOD CLOD <LOD <LOD CLOD <LOD CLOD <LOD CLOD cLOD CLOD CLOD CLOD CLOD cLOQ CLOQ
cLOD CLOD cL O D CLOD CLOD CLOD CLOD cL O D CLOD cL O D CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD
L O D = limit o f detection (0.503 pgflcg) L O Q = limit of quantitation (2.0 fig/kg)
Anaerobic Live Sterile
CLOD CLOD CLOD CLOD CLOD CLOD cLOQ cLOQ cLOD CLOD CLOQ cLOQ cLOQ CLOQ
cLOD CLOD CLOD cLOD cL O D CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD cLOQ
Fridayt O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 162-
p. 163 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-:08
Measured Concentrations of 8-2 FTA (pg/kg) in Soils Treated with.
Soil Type InceDtisol
Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24
Aerobic Live Sterile
<LOQ <LOQ CLOD <LOD <LOQ <LOQ
5.5 4.8 18.0 13.0 73.7 75.7 162.8 144.6 163.8 126.8 133.2 140.6 193.0 205.7 213.7 226.5 202.5 221.7
<LOD <LOD <LOD <LOD <LOD CLOD CLOD <LOD CLOD CLOD
cLOQ
CLOD 2.9
cLOQ
2.8 CLOQ
cLOQ
cLOQ
LOD = limit of detection (0.503 pg/kg) LOQ = limit of quantitation (2.0 pg/kg)
Anaerobic Live Sterile
CLOD CLOD CLOQ
CLOD
CLOD CLOD
6.6 <LOQ
2.5 2.6 6.1 5.9 8.5 5.3
CLOD CLOD CLOD CLOD CLOD CLOD
cLOD
CLOD CLOD CLOD CLOD CLOD CLOD CLOQ
.
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 163 -
p. 164 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFNA (ng/kg) in Soils Treated witfa^ *3
Soil Type Ultisol
Sample Interval Day 0 Week 1 Week 2 Week4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
Aerobic Live Sterile
Anaerobic Live Sterile
<LOQ <LOQ
7.0 7.2 11.5 12.3 17.6 16.7 24.0 21.5 28.0 30.0 28.5 30.7 33.2 30.1 8.6 15.7 23.8 26.2 29.8 24.4 31.0 29.5
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ , 6.5
7.0 9.2 9.5 15.7 13.7 15.4 21.5 23.4 22.4 30.0 25.5
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOD = limit o f detection (0.124 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg)
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBl.
W ildlife International, Ltd.
-164-
p. 165 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFNA (pg/kg)
in Soils Trented with
J
Soil Type Mollisol
Sample Interval Day 0 Week 1 Week 2 Week 4 Month. 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
Aerobic Live Sterile
<LOQ <LOQ
5.8 6.0 5.7 8.3 10.6 12.3 18.1 17.2 22.4 22.5 21.2 20.0 17.3 11.1 19.5 18.4 14.6 25.8 20.7 17.1 25.7 34.7
<LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
Anaerobic Live Sterile
<LOQ <LOQ
4.2 4.3 5.8 4.6 4.3 5.9 6.5 8.2 12.1 9.3 16.0 12.1
<LOD <LOD <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOD = limit o f detection (0.124 pg/kg) LOQ = limit of quantitation (2.0 pg/kg)
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 165-
p. 166 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFNA in Soils Treated withC.
Soil Type Alfisol
S am ple Interval ' Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
Aerobic Live Sterile
<LOQ <LOQ
3.3 4.0 4.6 3.9 6.6 7.7 10.4 16.2 10.6 13.5 8.9 15.9 17.6 23.3 13.4 17.5 23.1 15.2 15.7 17.7 25.3 22.0
<LOD <LOD <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOD <LOQ <LOQ
LOD = limit of detection (0.124 pg/kg) LOQ = limit of quantitation (2.0 jig/kg)
Anaerobic Live Sterile
<LOQ <LOQ <LOQ
2.4 2.7 3.2 4.8 4.9 3.6 6.6 8.9 9.2 11.2 7.9
<LOQ <LOQ <LOQ <LOD <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
F riday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 166-
p. 167 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFNA (pg/kg)
in Soils Treated with(T
T
Soil Type Incentisol
Sample Interval Day 0 Week 1 Wed: 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
Aerobic Live Sterile
<LOQ <LOQ
20.3 25.1 29.0 33.4 36.9 35.2 62.2 63.9 59.4 61.9 67.9 79.9 79.9 79.5 71.5 70.7 73.9 74.1 79.2 80.6 96.5 104.2
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOD = limit o f detection (0.124 pg/kg) LOQ = limit of quantitation (2.0 pg/kg)
Anaerobic Live Sterile
<LQ <LOQ
9.5 7.2 13.1 13.5 34.9 21.8 24.8 26.6 28.7 35.7 34.0 36.1
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
Friday, O ctober 12, 2001
Company Sanitized. Does not contain TSCA CBI.
,W ildlife International Ltd.
- 167-
p. 168 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFDA (jrg/kg)
in Soils Treated w ith
j.
Soil Type Ultisol
Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18
M onth 24
Aerobic Live Sterile
<LOQ <LOQ
4.6 4.8 7.6 8.4 10.7 11.9 18.0 18.5 26.6 28.2 30.4 35.5 37.9 39.3 9.2 20.9 33.3 34.0 48.1 36.5 50.7 44.3
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOD = limit o f detection (0.114 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg)
Anaerobic Live Sterile
<LOQ <LOQ
4.8 5.2 6.9 7.3 9.3 8.5 13.2 15.0 25.4 28.4 21.9 19.6
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
Thursday, N ovem ber OS, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 168 -
p. 169 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Soil Type Mollisol
Measured Concentrations of PFDA (jttg/kg)
in Soils Trested with^
J
iSample Interval
Aerobic U ve Sterile
Anaerobic Live Sterile
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ
3.4 3.7 4.7 5.2 6.2 6.2 9.9 10.1 14.6 16.4 17.3 17.6 16.6 12.4 19.7 18.6 14.6 27.7 22.0 21.4 45.4 48.5
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
2.0 <LOQ <LOQ <LOQ <LOQ
2.1 <LOQ <LOQ
<LOQ <LOQ
3.7 3.7 5.4 4.7 6.5 6.7 11.0 10.7 17.4 14.2 21.0 17.5
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOD = limit o f detection (0.114 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg)
Thursday, N ovem ber 08, 2007
Company Sanitized. Does not contain TSCA CBl.
W ildlife International, Ltd.
- 169-
p. 170 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 1 1 2 E-1 0 8
Measured Concentrations of PFDA (jig/kg)
in Soils Trested with
3
S am ple
Aerobic
Anaerobic
Soil Type_______Interval_________ L ive Sterile_____ Live Sterile
Alfisol
DayO Weekl Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24
<LOQ <LOQ <LOQ
2.1 2.9 3.0 4.4 4.8 8.1 9.2 8.4 9.8 9.3 12.5 17.1 19.7 12.9 16.1 26.3 15.3 23.3 20.7 37.6 36.8
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ
<LOQ <LOQ <LOQ
2.4 3.1 3.6 5.4 5.6 4.8 8.7 12.9 13.2 14.6 12.2
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <l o q <LOQ <LOQ <LOQ
LOD = limit o f detection (0.114 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg)
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 170-
p. 171 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Soil Type Inceotisol
Measured Concentrations ofPFDA (ng/kg)
in Soils Treated w iti
3
Sample Interval
Aerobic Live Sterile
Anaerobic Live Sterile
Day 0 Week I Week2 Week4 Month. 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24
<LOQ <LOQ
4.5 5.4 8.1 8.3 15.6 15.6 34.3 29.8 43.5 50.4 52.3 68.8 66.7 57.2 62.0 69.8 62.9 66.0 81.4 77.9 86.7 " 89.6
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
2.1 <LOQ
2.6 <LOQ
3.1 3.1 2.9 2.3 2.3 2.0
<LOQ <LOQ
5.3 4.5 7.5 7.8 20.9 11.5 21.4 22.9 33.7 39.3 47.0 41.0
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
2.7 <LOQ
LOD = limit of detection (0.114 pg/kg) LOQ = limit of quantitation (2.0 pg/kg)
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-171 -
p. 172 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFUA (jig/kg) in Soils Treated witb(~
Soil Type Ultisol
-
Sample Interval Day 0 Week 1 Week2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
Aerobic Live Sterile
<LOQ <LOQ <LOQ <LOQ
3.0 3.3 5.1 4.7 8.5 8.0 13.2 15.0 16.0 16.4 18.8 17.0 3.6 10.5 15.1 15.5 19.2 16.1 22.5 22.7
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
Anaerobic Live Sterile
<LOQ <LOQ <LOQ <LOQ
2.7 2.6 4.1 3.9 6.9 8.2 15.5 13.1 18.2 17.6
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
*
LOD = limit of detection (0.128 ng/kg) LOQ = limit o f quantitation (2.0 fig/kg)
Friday, O ctober 22, 2007
Company Sanitized. Does not contain TSCA CBI.
Wildlife International, Ltd.
- 172-
p. 173 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFUA (fig/kg) in Soils Treated with
Soil Type Mollisol
Sample Interval DayO Week 1 Week2 Week4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
Aerobic Uve Sterile
<LOQ <LOQ <LOQ <LOQ <LOQ
2.1 2.7 2.7
6.0 5.7 10.0 10.8 9.5 9.6 8.2 4.6 8.8 8.6 6.1 11.6 10.4 9.2 14.5 21.3
<LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOD <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
Anaerobic Live Sterile
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
2.0 2.5 3.5 6.0 4.7 6.8 5.5
<LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOD
<LOD <LOQ <LOQ <LOQ <LOD
LOD = limit of detection (0.128 (ig/kg) LOQ = limit o f quantitation (2.0 pg/kg)
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
.W ildlife International, L td
- 173-
p. 174 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFUA (pg/kg) in Soils Treated withf_ 3
Sample Soil Type________ Interval
Alfisol
DayO Week 1
Week 2
Week 4
Month 2
Month 4
Month 6
Month 9
Month 12
Month 15
Month 18
Month 24
Aerobic Live Sterile
<LOD <LOD
<LOQ
cL O Q
<LOQ CLOQ
2.0 2.3 4.6 6.1 4.1 6.2 3.2 6.2 7.3 11.3 7.6 9.2 13.5
5.8
7.0 7.2
14.4
10.1
<LOD CLOD <L0Q cLOQ CLOD CLOD CLOD CLOD <LOD CLOD <LOQ CLOD CLOD CLOQ cLOQ CLOD CLOQ CLOQ
Anaerobic Live Sterile
CLOD CLOD CLOQ cL O Q CLOQ CLOQ
cLOQ cLOQ cLOQ
3.0 3.1 3.3 4.5 3.5
cLOD CLOD cLOD CLOD cL O Q cL O Q cL O D cLOD cL O Q CLOD CLOD CLOD CLOQ CLOQ
LOP = limit o f detection (0.128 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg)
Fridayt O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
Wildlife InternationalLtd.
-174-
p. 175 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFUA ( in Soils Treated with
Sample
Aerobic
Anaerobic
Soil Type________ Interval___________ Live Sterile______ Live Sterile
InceDtisol
Day 0 Week 1 Week2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ
<LOQ
<LOQ
2.4 3.9 4.3 7.1 6.0 19.0 19.8 18.7 18.8 20.8 28.0 29.4 28.2 25.9 29.3 26.1 25.7 34.9 33.0 40.5 45.3
<LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ CLOQ <LOQ
<LOD <LOD
cLOQ
<LOQ
3.4 3.5 9.6 6.3 14.1 14.2 17.1 19.1 22.2 21.1
<LOD <LOD cLOD CLOD cL O D
cLOQ
cLOQ CLOD cLOQ CLOQ cLOQ CLOD CLOQ cLOQ
LOD = limit o f detection (0.128 ng/kg) LOQ = limit of quantitation (2.0 pg/kg)
Friday, O ctober 12, 2907
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 175 -
p. 176 project no : 112E-ios
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 8-2 FTOH (pg/kg)
in Soils Treated w ith
D
Sample Soil Type________ Interval_______
Aerobic Live Sterile
Ultisol
DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24
<LOD <LOD CLOD CLOD cLOQ cLOQ CLOD CLOD CLOQ cL O Q cLOQ cLOQ CLOQ CLOQ cLOQ cLOQ CLOQ CLOD CLOQ cLOQ <LOQ <LOQ CLOD CLOD
<LOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD
Anaerobic Live Sterile
cLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD cLOD cLOD CLOD CLOD
cLOD CLOD <LOD CLOD cL O D CLOD cL O D CLOD cLOD CLOD cL O D cLOD cL O D CLOD
LOD = limit o f detection (22.3 pg/kg) LOQ = limit of quantitation (100 pg/kg)
M onday, O ctober IS , 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 176-
p. 177 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
5Measured Concentrations of 8-2 FTOH g/kg) in Soils Treated w ith f
Soil Type Mollisol
Sample Interval DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month IS Month 24
Aerobic Live Sterile
<LOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD <LOD CLOD CLOD CLOD CLOD CLOD <LOD CLOD CLOD CLOQ CLOD <LOD
<LOD CLOD CLOD CLOD CLOD CLOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD
LOD = limit of detection (22.3 jig/kg) LOQ = limit o f quantitation (100 ;ig/kg)
Anaerobic Live Sterile
CLOD CLOD cLOQ CLOQ CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD
CLOD CLOD cLOD CLOD cLOD CLOD cLOD CLOD cLOD CLOD CLOD cLOD CLOD CLOD
M onday, O ctober IS , 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 177-
p. 178 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 8-2 FTOH (fig/kg) in Soils Treated withQ
Sample
Aerobic
Anaerobic
Soil Type________ Interval___________ Live Sterile______ Live Sterile
Alfisol
DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
CLOD <LOD CLOD CLOD cLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD CLOD
<LOD <LOD CLOD CLOD
cLOQ CLOD
cL O D cL O D cLOD CLOD cL O D CLOD CLOD cLOD cL O D CLOD CLOD CLOD CLOD CLOD CLOD cL O D
<LOD
cL O D CLOD CLOD CLOD cL O D CLOD cLOD CLOD CLOD CLOD cLOQ cLOD CLOD CLOD
cL O D cLOD CLOD CLOD cLOD cLOD CLOD CLOD CLOD <LOD CLOD CLOD CLOD CLOD
LOD = limit o f detection (22.3 |ig/kg) LOQ = limit o f quantitation (100 pg/kg)
M onday, O ctober IS , 207
Company Sanitized. Does not contain TSCA CBl.
W ildlife International, Ltd.
- 178-
p. 179 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 1 12E-108
Measured Concentrations of 8-2 FTOH (pg/kg)
in Soils Treated with ^
~1
Soil Type InceDtisol
Sample Interval Day 0 Week 1 Week 2 Week 4 Month2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
Aerobic Uve Sterile
<LOD <LOD <L0D <LOD <LOD <LOD
<L0Q <LOQ
174 125 148 138 <LOQ 140 100 <LOQ 131 165 173 121 <LOQ <LOQ <LOQ <LOQ
<LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD
LOD = limit o f detection (22.3 pg/kg) LOQ = limit of quantitation (100 pg/kg)
Anaerobic Live Sterile
<LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOD <LOD <LOD <LOD <L0D <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD
M onday, O ctober IS , 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 179-
p. 180 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 8-2 FTOH Qtg/L) in Headspaces Gases from Chambers Treated with jf^
1
Sample
Aerobic
Anaerobic
Soil Type________ Interval____________Live Sterile______ Live Sterile
Ultisol
DayO Week 1 Week 2 Week 4 Month2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOQ = limit of quantitation (12.5 pg/L of headspace)
<LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
. M onday, O ctober 15, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 180-
p. 181 Project No.: 112E-108
Wildlife InternationalLtd.
Project Number 112E-108
Measured Concentrations of 8-2 FTOH (fig/L) in Headspaces Gases from Chambers Treated w ith
J
Soil Type
Sample Interval
Aerobic Live Sterile
Mollisol .
Day 0 Week 1 Week 2 Week 4 Month. 2 Month 4 Month. 6 Mbnth9 Month 12 Month 15 Month IS Month. 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOQ = limit of quantitation (12.5 pg/L of headspace)
Anaerobic Live Sterile
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
M onday, O ctober 15, 2907
Company Sanitized. Does not contain TSCA CBI.
W^ildlife International, Ltd.
-181 -
p. 182 project no.: 112E-108
Wildlife InternationalLtd.________________ Project Number 112E-108
Measured Concentrations of 8-2 FTOH (pg/L) in Headspaces Gases from Chambers Treated with Q
Sample Soil Type________ Interval_______
Aerobic Live Sterile
Alfisol
DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOQ = limit of quantitation (12.5 |ig/L of headspace)
Anaerobic Sterile
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
M onday, O ctober IS , 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 182-
p. 183 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 8-2 FTOH (fig/L) in Headspaces Gases from Chambers Treated with(T
Soil Type
Sample Interval
Aerobic Live Sterile
InceDtisol
DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOQ = limit ofquantitation (12.5 pg/L ofheadspace)
Anaerobic Live Sterile
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
M onday, O ctober IS , 2097
Company Sanitized. Does not contain TSCA CBI.
,W ildlife International Ltd.
- 183 -
p. 184 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 7-2 s FTOH (pg/kg) in Soils Treated with{^
Sample
Aerobic
Anaerobic
Soil Type________ Interval___________ Live Sterile______ Live Sterile
Ultisol
Day 0. Month 2 Month 6 Mouth9 Month 12 Month 15 Month IS Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ 133.0 166.1 <LOQ <LOQ <LOQ <LOQ 115.6 <LOQ <LOQ <LOQ <LOQ
LOQ = limit of quantitation (100 pg/kg)
Thursday, N ovem ber Ob, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 184-
p. 185 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 7-2 s FTOH (p.g/kg)
in Soils Treated withC
^
Sample
Aerobic
Anaerobic
Soil Type________ Interval___________ Live Sterile______ Live Sterile
Mollisol
Day 0 Month 2 Month 6
<LOQ <LOQ <LOQ <LOQ
a o' oo V1
Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <L0Q <LOQ
LOQ = limit of quantitation (100 pg/kg)
Thursday, Novem ber OS, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 185 -
p. 186 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 7-2 s FTOHQig/kg) in Soils Treated with.
' Sample Soil Type________ Interval
Alfisol
Day 0
Month 2
Month 6
Month9
Month 12
Month 15
Month 18
Month 24
Aerobic Live Sterile
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
Anaerobic Live Sterile
LOQ = limit of quantitation (100 pg/kg)
Thursday, N ovem ber 08, 2007
Company Sanitized. Does not contain TSCA CBI.
Wildlife International, Ltd.
- 186-
p. 187 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 7-2 s FTOH (pg/kg)
in Soils Treated w ith
J
Sample
Aerobic
Anaerobic
Soil Type_______ Interval___________ Live Sterile______ Live Sterile
Inceutisol
Day 0 Month 2 Month 6 Month 9 Month 12 Month IS Month IS Month 24
<LOQ <LOQ <LOQ <LOQ 278.3 278.3 192.8 464.2 193.0 118.9 173.2 182.2 <LOQ <LOQ <LOQ <LOQ
LOQ = limit of quantitation (100 pg/kg)
Thursday, N ovem ber 08,2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 187-
p. 188 projectn o ; i h e ios
Wildlife International, Ltd._________________ Project Number 1 1 2 E- 1 0 8
Measured Concentrations of 7-3 A dd (fig/kg)
in Soils Treated with
~J
Sample Soil Type________ Interval
Ultisol
Day 0
Month2
Month 6
Month9
Month 12
Month 15
Month 18
Month24
Aerobic Live Sterile
<LOQ <LOQ
6.6 9.1 16.3 21.4 31.3 28.7 41.7 33.4 34.1 40.1 27.8 17.0 54.3 56.2
Anaerobic Live__Sterile_
LOQ - limit of quantitation (2.0 ng/kg)
W ednesday, M arch 1 2 ,200S
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 188 -
p. 189 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 7-3 Acid Qig/kg)
in Soils Treated with f
'l
Soil Type Mollisol
Sample Interval Day 0 Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month24
Aerobic Live Sterile
<LOQ <LOQ <LOQ <LOQ
2.4 2.7 2.7 2.9 5.0 4.8 6.6 9.0 3.5 3.4 8.9 6.6
LOQ = limit o f quantitation (2.0 ng/kg)
Anaerobic Live Sterile
W ednesday, M arch 12,2008
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 189-
p. 190 Project no.: 112E-108
Wildlife International, Ltd._________________ Project Number B1 1 2 -1 0 8
Measured Concentrations of 7-3 Acid (p.g/kg) in Soils Treated w ith
Soil Type Alfisol
Sample Internai Day 0 Monili 2 Month6 Mondi 9 Mondi 12 Mondi 15 Month 18 Mondi 24
Aerobic Live Sterile
<LOQ <LOQ <LOQ <LOQ
3.2 2.5 3.2 2.5 4.9 6.1 7.6 8.9 8.1 6.4 6.6 7.3
Anaerobic Live Sterile
LOQ = limit of quantitation (2.0 |ig/kg)
W ednesday, M arch 12,2008
Company Sanitized. Does not contain TSCA CBI.
Wildlife International, Ltd.
- 190-
p. 191 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 7-3 Acid Qig/kg)
in Soils Treated with(_
^
Soil Type Inceotisol
'
Sample Interval Day 0 Month2 Month6 Month9 Month 12 Month 15 Month 18 Month24
Aerobic
. Anaerobic
Live Sterile______ Live Sterile
<LOQ
<LOQ
16 .7
9.6 67.7 81.6 128.3 153-2 277.0 246.2 260.7 289.7 282.3 2122
3 5 5 .2
321.9
LOQ = limit of quantitation (2.0 pg/kg)
W ednesday, M arch 1 2 ,20BS
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 191 -
p. 192 Project No.: 112E-108
Wildlife International, Ltd,______________Project Numbers 112E-108,109,110
Measured Concentrations of PFOA (pg/kg) in Untreated and Fortified Soils
Sample
Untreated Controls
Fortified Recoveries
Soil Type________ Interval______ Aerobic Anaerobic_____ Aerobic______ Anaerobic
Ultisol
DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
9.5 7.8 8.6 9.1 9.2 9.2 10.4 10.3 12.0 11.4 10.6 10.4 9.2 10.0 7.4 6.8
95% 7854 86% 91% 92% 92% 104% 103% 120% 114% 106% 104% 92% 100% 74% 68%
9.5 95% 9.2 92% 10.5 105% 11.3 113% 9.2 92% 9.8 98% 9.3 93% 9.0 90% 11.4 114% 10.8 108% 11.4 114% 11.3 113% 11.2 112% 11.5 115%
LOD = limit of detection (0.206 pg/kg) LOQ = limit of quantitation (2.0 pg/kg)
Friday, O ctober 12, 2007
Page 1 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 192 -
p. 193 Project No.: 112E-108
Wildlife International, Ltd.
P ro jectN um bersll2E -108,109,I10
Measured Concentrations of PFOA (pg/kg) in Untreated and Fortified Soils
Sam ple
U ntreated C ontrols
F ortified R ecoveries
S o il Type______ In terval_____ A erobic A naerobic____ A erobic____ A naerobic
Mollisol
Day 0 Week 1 Week 2 Week4 Month 2 Month 4
Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOD <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOQ
LOD = limit of detection (0.206 pg/kg) LOQ = limit of quantitation (2.0 pg/kg)
<LOD <LOD <LOD <LOQ <LOD
<LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD
8.9 9.3 9.7 10.0 8.8 9.4 9.1 9.0 10.7 11.2 13.2 12.1 13.1 12.0 11.4 11.6 9.2 10.4
89% 93% 97% 100%
88%
94% 91% 90% 107% 112% 132%
121%
131% 120% 114% 116% 92% 104%
9.3 10.1 8.5 9.6 8.5 8.9 9.5 9.9 12.2 10.9 14.0 13.9 14.5 14.3
93% 101% 83% 96% 85% 89% 95% 99% 122% 109% 140% 139% 143% 143%
Friday, O ctober 12, 2007
Page 2 o f4
- Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 193 -
p. 194 project n o .: i h e - ios
Wildlife International, Ltd.______________ Project Numbers 112E-108,109,110
Measured Concentrations of PFOA Qig/kg) in Untreated and Fortified Soils
S oil Type
Sam ple In terval
U ntreated C ontrols A erobic A naerobic
A lfisol
DayO
Weds; 1 Week 2 Week 4 Month2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24
<LOD <LOD <LOD CLOD cL O D CLOD CLOD CLOD cLOD CLOD cL O Q CLOQ CLOD CLOD cL O D CLOD CLOD CLOD CLOD CLOD CLOD
cLOD CLOD cLOD CLOD CLOD CLOD cL O D cL O D cLOD cLOD CLOD CLOD CLOD CLOD
LOD = limit of detection (0.206 pg/kg) LOQ= limit of quantitation (2.0 pg/kg)
F o rtified R ecoveries
A erobic
A naerobic
9.2 8.3 8.9
10.0
9.9 9.4
10.0 10.1 11.0
10.7
10.8
11.5
11.0 11.1
11.5 12.5 9.2 9.3
92% 83% 89% 100% 99% 94% 100% 101% 110% 107% 108% 115% 110% 111% 115% 125% 92% 93%
9.2 8.2 8.7 9.3 9.6
10.0
10.6
10.4 10.4 10.7
11.1
10.9 10.5
11.2
92% 82% 87% 93% 96% 100% 106% 104% 104% 107% 111% 109% 105% 112%
F riday, October 12, 2007
Page 3 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 194-
p. 195 Project No.: 112E-108
Wildlife International, Ltd.
ProjectNumbersll2E-108,109,110
Measured Concentrations of PFOA (fig/kg) in Untreated and Fortified Soils
S o il Type
Sam ple In terval
U ntreated C ontrols A erobic A naerobic
Inceotisol
Day 0 Week 1 Week2 Week4 Month 2 Month 4 Month 6 Month 9 Month 12 Month L5 Month IS Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOD = limit of detection (0.206 fj.g/kg) LOQ = limit of quantitation (2.0 jig/kg)
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
F ortified R ecoveries
A erobic
A naerobic
10.4 10.5 12.9 10.1 10.6 10.9 12.4 11.2 11.3 11.7 13.4 10.8 12.1 10.6 14.5 12.4 9.6 9.6
104% 105% 129% 101% 106% 109% 124% 112% 113% 117% 134% 10% 121% 106% 145% 124% 96% 96%
9.7 9.4 10.9 9.8 10.8 10.5 10.7 10.9 11.9 11.1 10.6 11.5 11.3 11.6
97% 94% 109% 98% 108% 105% 107% 109% 119%
111%
106% 115% 113% 116%
Friday, O ctober 1 2 ,2 0 0 7
Page 4 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 195 -
p. 196 project no .: 112E-108
Wildlife InternationalLtd._____________ Project Numbers 1 1 2 B-1 0 8 ,109,110
Measured Concentrations of 8-2 FTUA (ng/kg) in Untreated and Fortified Soils
Sam ple
U ntreated C ontrols
F ortified R ecoveries
S oil Type_______In terval_____ A erobic A naerobic____ A erobic_____A naerobic
Ultisol
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9
Month 12 Month 15 Month IS Month 24
<LOQ <LOD <LOD CLOD cL O D CLOD
cLOD
cLOD CLOD CLOD cLOD CLOD cLOD CLOD cLOD CLOD CLOD
cLOD cLOD
CLOD CLOD
cLOD CLOD cLOD CLOD cLOD cL O D cL O D cL O D cLOD CLOD CLOD CLOD CLOD CLOD
7.2 72% 6.5 65% 7.7 77% 7.9 79% 6.9 69% 6.9 69% 7.2 72% 7.2 72% 8.9 89% 8.0 80% 6.5 65% 7.3 73% 2.9 29% 5.4 54% 5.1 51% 4.0 40%
8.0 80% 7.4 74% 7.7 77% 8.7 87% 8.4 84% 8.2 82% 8.2 82% 7.9 79% 9.3 93% 7.6 76% 8.2 82%
8 .0 80%
6.5 65% 7.0 70%
LOD = limit of detection (0.119 ng/kg) LOQ = limit of quantitation (2.0 gg/kg)
Friday, O ctober 12, 2007
Page J o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 196-
p. 197 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers 112E-108,109,11 0
Measured Concentrations of 8-2 FTUA (jig/kg) in Untreated and Fortified Soils
Sam ple
U ntreated C ontrols
F ortified R ecoveries
S o il Type______ In terval_____ A erobic A naerobic____ A erobic_____Anaerobic
M ollisol ,
Day 0 W eek 1 W eek 2 W eek 4 M onth 2 M onth 4 M onth 6 M onth 9 M onth 12 M onth 15 M o n th 18 M onth 24
<LOD
<LOD '
<LOD CLOD CLOD CLOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD
CLOD CLOD cLOQ CLOD CLOD CLOD CLOD CLOD <LOD CLOD CLOD CLOD CLOD <LOD
9.2 9.0 8.1 8.5 9.1 9.5 9.0 9.1 8.8 8.7 10.0 9.4 10.2 8.2 7.8 9.0 5.9 6.3
92% 90% 81% 85% 91% 95% 90% 91% 88% 87% 100% 94% 102% 82% 78% 90% 59% 63%
7.3 8.8 7.7 8.7 7.7 9.8 8.0 8.0 9.7 7.8 9.9 10.7 10.0 8.5
73% 88% 77% 87% 77% 98% 80% 80% 97% 78% 99% 107% 100% 85%
LOD = limit of detection (0.119 pg/kg) LOQ = limit of quantitation (2.0 pg/kg)
Friday, O ctober 12, 2007
Page 2 o f4
Company Sanitized. Does not contain TSCA CBI.
,W ildlife International Ltd.
- 197-
p. 198 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers 112E-108,109,110
Measured Concentrations of 8-2 FTUA ((ig/kg) in Untreated and Fortified Soils
Sam ple
U ntreated C ontrols
F ortified R ecoveries
S o il Type_______In terval_____ A erobic Anaerobic____ A erobic_____A naerobic
Alfisol
DayO Week 1 Week 2 Week 4 Month. 2 Month 4-
Month 6 Month 9 Month 12 Month IS Month IS Month 24
<L0D <LOD <L0D CLOD cL O D CLOD CLOD CLOD cLOD cLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD cLOD <LOD
cLOD CLOD cLOD cLOD CLOD CLOD cLOD CLOD CLOD cLOD CLOD CLOD cLOD CLOD
8.4 84%
7.7 77% 8.6 86% 9.8 98% 10.5 105% 7.6 76% 7.5 75% 9.2 92% 9.6 96% 9.1 91% 6.6 66% 9.0 90% 8.4 84% 9.0 90% 8.0 80% 11.1 111% 6.2 62% 6.7 67%
8.9 7.5 9.3 9.5 9.2 8.9 8.6
9.4
10.9 10.8
8.1 8.3 10.3 8.3
89% 75% 93% 95% 92% 89% 86% 94% 109% 108% 81% 83% 103% 83%
LOD = limit of detection (0.119 pg/kg) LOQ = limit of quantitation (2.0 pg/kg)
,Friday O ctober 12, 2007
Page 3 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 198-
p. 199 Project No.: 112E-108
Wildttfe International, Ltd.
Project Numbers 1 1 2 E -] 0 8 ,1 0 9 ,1 1 0
Measured Concentrations of 8-2 FTUA (ng/kg) in Untreated and Fortified Soils
Sam ple
U ntreated C ontrols
F ortified R ecoveries
S o il Type______ Interval_____ A erobic A naerobic____ A erobic____ Anaerobic
InceD tisol
DayO Week 1 Week2 Week4 Month 2 Mdnth4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 2+
<LOD CLOD <LOD CLOD CLOD CLOD CLOD CLOD cLOD <LOD CLOD <LOD cLOD CLOD CLOD CLOD cLOD CLOD <L0D <LOD CLOD
CLOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD cLOD CLOD
11.0 9.6 10.8 8.3 8.8 9.7 9.4 7.9 8.0 8.9 10.1 8.1 7.0 6.8 7.4 6.4 5.3
5.2
110% 96% 108% 83% 88% 97% 94% 79% 80% 89% 101% 81% 70% 68% 74% 64% 53% 52%
7 .9 79% 8.1 81% 8.3 83% 7.5 75% 8.5 85% 7.8 78% 7.7 77% 8 .2 82% 7.6 76% 8.8 88% 6.6 66% 7.0 70% 6.1 61% 7.1 71%
LO D = lim it o f detection (0.119 pg/kg) L O Q = lim it o f quantitation (2.0 pg/kg)
Friday, O ctober 12, 2007
Page 4 o f4
Company Sanitized. Does not contain TSCA CBi.
W ildlife International, Ltd.
-199-
p. 200 Projectn 0.: 112E-108
Wildlife International, Ltd._____________ Project Numbers 1 1 2 E-1 0 8 ,109,110
Measured Concentrations of 8-2 FTA (jag/kg) in Untreated and Fortified Soils
S o il Type Ultisol
Sam ple In terval Day 0
Week 1 Week 2
Week 4
Month 2
Month 4
Month 6
Month 9
Month 12
Month 15 Month 18 Month 24
U ntreated C ontrols A erobic A naerobic
<LOD <LOD CLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD CLOD <LOD cL O D CLOD cL O Q cL O Q CLOD <LOD CLOD
cL O D cLOD CLOD cL O D cL O D cL O D CLOD CLOD CLOD CLOD CLOD CLOD cLOD c LOD
F ortified R ecoveries
A erobic
A naerobic
6.1 61% 5.8 58% 6.2 62% 6.9 69% 5.4 54% 5.5 55% 5.4 54% 5.3 53% 3.3 33% 2.4 24% 3.4 34% 3.7 37%
(1.4 )
(1.4) 4.1 41%
(1.7)
6.7 67% 6.6 W>% 7.0 70% 6.9 69% 6.1 61% 6.5 65% 5.3 53% 6.3 63% 7.2 72% 5.1 51% 4.8 48% 4.6 46% 3.5 35% 3.2 32%
L O D = lim it o f detection (0.503 pg/kg) L O Q = lim it o f quantitation (2.0 pg/kg)
V a lu e s in parentheses a r e < L O Q a n d > = L O D
Friday, O ctober 12, 2007
Page 1 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 200-
p. 201 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers 112E-108.,109>110
Measured Concentrations of 8-2 FTA (pg/kg) in Untreated and Fortified Soils
S oil Type Mollisol
Sam ple In terval DayO
Week 1 Week 2
Week 4
Month 2 Month. 4
Month 6
Month 9
Month 12
Month 15 Month 18 Month 24
U ntreated C ontrols A erobic A naerobic
F ortified R ecoveries
A erobic
A naerobic
<LOD <LOD
2.7 <LOD <LOD <LOD <LOQ <LOD <L0D <LOD <LOD <L0D <L0D <LOD <LOD <LOD <LOD <LOQ <L0D <LOD <LOD
<LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD
8.7 9.0 8.4 9.0 9.0 8.8 8.3 8.1 7.8 - 7.9 8.4 6.9 6.4
6 .2
5.0
6 .2
2.3 (1.71
87% 90% 84% 90% 90% 88% 83% 81% 78% 79% 84% 69% 64% 62% 50% 62% 23%
7.0 70% 8.7 87% 7.1 71% 7.6 76% 6.4 64% 8.3 83% 6.3 63% 7.0 70% 8.0 80% 7.1 71% 5.8 58% 6.5 65% 5.7 57% 5.2 52%
LOD = limit of detection (0.503 pg/kg) LOQ = limit of quantitation (2.0 pg/kg) Values in parentheses are <LOQ and >=LOD
Friday, O ctober 12, 2007
Page 2 o f4
Company Sanitized. Does not contain TSCA CBI.
.W ildlife International, Ltd
-201 -
p. 202 Project No.: 112E-108
Wildlife International, Ltd.______________Project Numbers 11ZE-108,109,no
Measured Concentrations of 8-2 FTA (pg/kg) in Untreated and Fortified Soils
Sam ple
U ntreated C ontrols
F o rtified R ecoveries
S o il Type_______In terval_____ A erobic A naerobic____ A erobic_____A naerobic
Alfisol
DayO
Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOD <LOD <LOD CLOD CLOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD <LOD <LOD <LOD cLOD cLOD CLOD cLOQ
cLOD CLOD cL O D CLOD cLOD CLOD cLOD cLOD cLOD CLOD CLOD cLOD CLOQ CLOQ
7.8 7.9
8.4 10.6 8.7 6.8 8.4 7.5 7.0 4.2 6.7 6.0 6.6 5.1 7.7 4.0 3.7
oo
78% 79% 78% 84% 106% 87% 68% 84% 75% 70% 42% 67% 60% 66% 51% 77% 40% 37%
8.8 88% 6.7 67% 8.5 85% 8.4 84% 8.4 84% 8.1 81% 7.7 77% 8.3 83% 8.8 88% 8.3 83% 6.7 67% 6.6 66%
7.3 73% 7.0 70%
LOD = limit of detection (0.503 pg/kg)
L O Q = lim it o f quantitation (2.0 pg/kg) V alues in parentheses are <L O Q and >=LO D
Friday, O ctober 12, 2007
Page 3 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 202-
p. 203 Project No.: 112E-108
Wildlife International, Ltd.
P roject N um bers 112E -108,109,110
Measured Concentrations of 8-2 FTA (fig/kg) in Untreated and Fortified Soils
Sample
Untreated Controls
Fortified Recoveries
Soil Type________ Interval______ Aerobic Anaerobic_____ Aerobic______ Anaerobic
InceDtisol
DayO
Week 1
Week2 -
Week 4
Month2
' Month 4
Month6
Month 9
Month 12
Month 15 Month 18 Month 24
CLOD CLOD <LOD <LOD CLOD CLOD CLOQ CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOQ CLOQ CLOQ CLOD CLOD CLOD cLOQ
CLOD CLOD CLOD <LOD
CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD
10.7 9.6 9.8 7.6 7.0 8.4 7.1 6.3 5.5 5.8 5.3 4.5 4.3 3.7 3.4 3.4 2.1 (1.81
107% 96% 98% 76% 70% 84% 71% 3% 55% 58% 53% 45% 43% 37% 34% 34% 21%
7.8 78% 7.2 72% 7.0 70% 6.7 7% 7.2 72% 7.6 76% 5.9 59% 6.6 66% 4.8 48% 6.3 63% 3.6 36% 3.4 34% 2.6 26% 3.5 35%
LOD = limit ofdetection (0.503 pg/kg) LOQ = limit ofquantitation (2.0 jig/kg) Values in parentheses are <LOQ and >=LOD
Friday, O ctober 12, 2007
Page 4 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-203 -
p. 204 Project No.: 112E-108
Wildlife International, Ltd.
P roject N um bers 112E -108, 109,110
Measured Concentrations of PFNA (fig/kg) in Untreated and Fortified Soils
Soil Type Ultisol
Sample
Untreated Controls
Fortified Recoveries
Interval______ Aerobic Anaerobic_____ Aerobic
Anaerobic
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
9.4 94% 7.8 78% 9.7 97% 9.8 98% 9.5 95% 9.8 98% 10.4 104% 10.1 101% 11.4 114% 11.4 114% 10.3 103% 10.6 106% 7.0 70 % 10.3 103% 7.9 79% 6.7 67 %
10.0 100% 9.9 9 9 % 9.9 99% 10.7 107% .9.7 97% 10.3 103% 10.3 103% 9.6 96% 11.2 112% 11.0 110% 11.6 116% 11.7 117% 10.8 108% 11.6 116%
LOD --limit of detection (0.124 jig/kg) LOQ = limit of quantitation (2.0 jig/kg)
F riday, O ctober 12, 2607
Page 1 o f4
Company Sanitized. Does not contain TSCA CB1.
W ildlife International, Ltd.
-204-
p. 205 Project No.: 112E-108
Wildlife International, Ltd. _____________ P ro je c t N u m b e rs 112E -108,109,110
Measured Concentrations of PFNA (pg/kg) in Untreated and Fortified Soils
Soil Type Mollisol
Sample Interval DayO
Week 1 Week 2
Week 4
Month 2
Month 4
Month 6
Month 9
Month 12
Month 15 Month 18 Month 24
Untreated Controls Aerobic Anaerobic
<LOD <LOD <LOD CLOD CLOD CLOD CLOD cLOQ <LOD CLOD CLOD CLOD CLOD CLOD cLOQ CLOQ CLOD CLOD CLOD CLOD CLOD
CLOD CLOD cL O Q CLOD CLOD CLOD cLOD cLOD CLOD CLOD cLOD CLOD CLOD cLOD
LOD = limit of detection (0.124 pg/kg) LOQ = limit of quantitation (2.0 pg/kg)
Fortified Recoveries
Aerobic
Anaerobic
9.4 9.6 9.7 9.7 9.5 9.9 10.2 8.6 10.7 10.6 12.2 11.9 12.0 11.7 10.0 11.2 8.1 8.3
94% 96% 97% 97% 95% 99% 102% 86% 107% 106% 122% 119% 120% 117% 100% 112% 81% 83%
9.6 10.1 8.9 9.8 9.6 9.9 9.3 9.8 10.7 10.1 11.6 12.8 12.2 11.9
96% 101% 89% 98% 96% 99% 93% 98% 107% 101% 116% 128% 122% 119%
Friday, O ctober 12, 2007
Page 2 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-205 -
p. 206 Project No.: 112E-108
Wildlife International, Ltd.
P roject N um bers 1 12E -108,109,110
Measured Concentrations of PFNA (pg/kg) in Untreated and Fortified Soils
Sample
Untreated Controls
Fortified Recoveries
Soil Type________ Interval______ Aerobic Anaerobic_____ Aerobic______ Anaerobic
Alfisol
DayO Week 1 Week 2 Week 4 Month. 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24
<LOD <LOD <LOD <LOD <LOD CLOD cL O D CLOD cLOD CLOD CLOD CLOD CLOQ cL O Q CLOQ CLOQ cLOQ cLOQ CLOD CLOD CLOD
cLOD CLOD cLOD CLOD cL O D CLOD CLOD cLOD cLOD CLOD CLOD CLOD CLOQ cL O Q
9.8 9.3 9.4 11.2 10.3 9.1 10.3 10.7 11.3 11.0 10,0 11.4 10.8 11.4 11.3 12.3 8.7 8.6
98% 93% 94% 112% 103% 91% 103% 107% 113% 110% 100% 114% 108% 114% 113% 123% 87% 86%
9.7
8.1
9.4 10.1 9.8 10.1 10.3 10.0 11.0 11.0 10.7 10.7 11.5 10.2
97% 81% 94% 101% 98% 101% 103% 100% 110% 110% 107% 107% 115% 102%
LOD = limit of detection (0.124 ng/kg) LOQ = limit of quantitation (2.0 ig/kg)
Friday, O ctober 12, 2007
Page 3 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 206-
p. 207 project n 0.: 112E-108
Wildlife International, Ltd.
P ro ject N um bers 112E -108,109,110
Measured Concentrations of PFNA (ug/kg) in Untreated and Fortified Soils
Soil Type Incentisol
Sample Interval
DayO
Weds 1 Weds 2 Week 4
Month 2 Month 4 Month 6
Month 9
Month 12
Month 15 Month 18 Month 24
Untreated Controls Aerobic Anaerobic
<LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOD <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOD <LOQ <LOD
<LOD CLOD CLOD CLOD cLOQ cLOQ cLOQ cLOQ cLOQ <LOD cLOQ cLOQ cLOQ CLOQ
Fortified Recoveries
Aerobic
Anaerobic
12.4 11.6 13.0 10.6 10.7 10.7 12.5 10.7 11.2 11.9 14.0 10.5 10.4 10.0 13.0 10.9
8.9 8.8
124% iis% 130% 106% 107% 107% 125% 107% 112% 119% 140% 105% 104% 100% 130% 109%
89% 88%
10.2 10.0 10.8 9.8 10.3 10.2 10.1 10.7
11.1 11.1 11.0 10.8 10.1 10.8
102% 100% 108% 98% 103% 102% 101% 107% 111% 111% 110% 108% 101% 108%
LOD = limit ofdetection (0.124 ug/kg) LOQ = limit of quantitation (2.0 pg/kg)
Friday, O ctober 12, 2007
Page 4 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-207-
p. 208 Project No.: 112E-108
WildUfe International, Ltd.__________________ P ro je c t N u m b ers 1 1 2 E -1 0 8 ,1 0 9 ,1 1 0
Measured Concentrations of PFDA (pg/kg) in Untreated and Fortified Soils
Soil Type Ultisol
Sample Interval DayO
Week 1 Week 2
Week4
Month 2
Month 4
Month 6
Month 9
Month 12
Month IS Month IS Month 24
Untreated Controls
Fortified Recoveries
Aerobic Anaerobic_____ Aerobic______ Anaerobic
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
9.3 93% 7.4 74% 8.9 89% 8.6 86% 8.8 88% 8.9 89% 9.4 94% 9.3 93% 10.9 109% 10.9 109% 9.6 96% 10.0 100% 5.2 52% 9.5 95% 8.4 84% 7.0 70%
9.2 9.1 9.0 9.7 9.4 9.2 9.3 8.0 10.4 10.2 11.0 10.9 9.6 10.4
92% 91% 90% 97% 94% 92% 93% 80% 104% 102% 110% 109% 96% 104%
LOD = limit,ofdetection (0.114 pg/kg) LOQ = limit ofquantitation (2.0 pg/kg)
F riday, O ctober 12, 2007
Page 1 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-208-
p. 209 Project No.: 112E-108
Wildlife International, Ltd.
P roject N um bers ]12E-108,109,110
Measured Concentrations of PFDA (pg/kg) in Untreated and Fortified Soils
Soil Type Mollisol
Sample Interval DayO
Week 1
Week 2
Week 4
Month.2
Month 4
Month 6
Month 9
Month 12
Month 15 Month 18 Month 24
Untreated Controls Aerobic Anaerobic
<LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ
<LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD
LOD = limit ofdetection (0.114 |ig/kg) LOQ = limit ofquantitation(2.0 jig/kg)
Fortified Recoveries
Aerobic
Anaerobic
10.0 10.0 9.8 10.4 9.7 10.1 9.8 8.1 9.9 9.7 11.7 10.9 12.1 11.2 9.2 10.2 7.6 8.0
100% 100% 98% 104% 97% 101% 98% 81% 99% 97% 117% 109% 121% 112% 92% 102% 76% 80%
9.2 9.9 8.2 9.5 9.2 9.3 9.0 9.0 10.4 9.2 11.4 12.1 11.2 10.9
92% 99% 82% 95% 92% 93% 90% 90% 104% 92% 114% 121% 112% 109%
Friday, O ctober 12, 2007
Page 2 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-209-
p. 210 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers ii2 E -io 8 ,io 9 ,n o
Measured Concentrations of PFDA (pg/kg) in Untreated and Fortified Soils
Alfisol
Sample Interval Day 0 Week 1
Week 2
Week 4
Month 2
Month 4
Month 6
Month 9
Month 12
Month IS Month 18 Month 24
Untreated Controls
F ortified Recoveries
Aerobic Anaerobic_____ Aerobic______ Anaerobic
cLOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD <LOD <LOD CLOD CLOD CLOD CLOD CLOQ CLOD <LOD CLOD CLOD
CLOD cLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD CLOQ
9.3 9.3 9.4 11.0 10.1
8.6
9.0 10.3 11.9 11.2 9.3 10.9 10.7 11.2 10.9 13.3 9.2 9.3
93% 93% 94% 110* 101% 86% 90% 105% 119% 112% 93% 109% 107% 112% 109% 133% 92% 93%
9.2 7.4 8.7 9.3 9.0 9.5 9.7 9.6 11.3 11.4 9.0 9.6 . 11.6
8 .6
92% 74% 57% 93% 90% 95% 97% 96% 113% 114% 90% 96% 116% 86%
LOD = limit of detection (0.114 pg/kg) LOQ = limit of quantitation (2.0 pg/kg)
F riday, O ctober 12, 2007
Page 3 o f4
Company Sanitized. Does not contain TSCA CBI.
Wildlife InternationalLtd.
- 210-
p. 211 Project No.: 112E-108
Wildlife International, Ltd.
P roject N um bers 112E -108,109,110
Measured Concentrations of PFDA (pg/kg) in Untreated and Fortified Soils
Soil Type InceDtisol
Sample Interval Day 0 Week 1
Week2
Week4 Month 2
Month4
Month 6
Month 9
Month 12
Month 15 Month 18 Month 24
Untreated Controls Aerobic Anaerobic
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ
LOD = limit of detection (0.114 (ig/kg) LOQ = limit of quantitation (2.0 pg/kg)
Fortified Recoveries
Aerobic
Anaerobic
13.9 12.4 14.6 11.6 11.3 11.6 12.1 11.5 12.0 13.3 14.1 10.8 10.7 9.2 12.8 10.9 9.5 9.2
139% 124% 146% 116% 113% 116% 121% 113% 120% 133% 141% 108% 107% 92% 128% 109% 95% 92%
9.9 9.7 10.7 9.6 10.8 10.5 10.1 10.7 11.8 12.2 10.8 11.6 9.6 10.6
99% 97% 107% 96% 108% 103% 101% 107% 118% 122% 108% 116% 96% 106%
F riday. O ctober 12, 2007
Page 4 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-211 -
p. 212 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers 112E-108,109,110
Measured Concentrations of PFUA (pg/kg) in Untreated and Fortified Soils
Sample
Untreated Controls
Fortified Recoveries
Soil Type________ Interval_______Aerobic Anaerobic_____ Aerobic______ Anaerobic
Ultisol
DayO Weekl Week 2 Week 4 Month 2 Month 4
Month 6 Month 9 Month 12 Month IS Month 18 Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ
8.5 6.9 8.2 8.2 7.6 7.8 8.6 7.4 10.2 10.1 9.2 9.3 4.1 8.4 7.2 6.2
85% 69% 82% 82% 76% 78% 86% 74% 102% 101% 92% 93% 41% 84% 72% 62%
8.5 85% 8.8 88% 8.5 85% 8.4 84% 8.8 88% 8.6 86% 9.1 91% 7.0 70% 9.8 98% 9.5 95% 9.4 94% 9.5 95% 8.8 88% 9.9 99%
LOD --limit of detection (0.128 pg/kg) LOQ = limit of quantitation (2.0 gg/kg)
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-212 -
p. 213 Project No.: 112E-108
Wildlife International, Ltd.
P ro ject N um bers 112E -108,109,110
Measured Concentrations of PFUA (fig/kg) in Untreated and Fortified Soils
Soil Type Mollisol
Sample Interval DayO
Week 1
Week!
Week 4 Month 2
Month4
Month6
Month 9
Monti 12
Month 15 Month 18 Month 24
Untreated Controls Aerobic Anaerobic
<LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD
<LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD
LOD = limit of detection (0.128 pg/kg) LOQ = limit of quantitation (2.0 pg/kg)
Fortified Recoveries
Aerobic
Anaerobic
9.5 9.6 8.7 9.3 9.0 9.2 8.7 6.9 8.4 8.4 10.1 10.1 9.5 8.7 7.4 8.8 6.1 6.3
95% 96% 87% 93% 90% 92% 87% 69% 84% 84% 101%
101%
95% 87% 74%
88%
61% 63%
8.2 82% 8.6 86% 6.7 67% 7.6 76% 6.3 63% 7.1 71% 6.3 63% 8.0 80% 8.5 85% 7.5 75% 9.6 96% 9.4 94% 9.8 98% 7.9 79%
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-213 -
p. 214 Project No.: 112E-108
Wildlife International, Ltd.
P roject N um bers 112E -108,109,110
Measured Concentrations of PFUA (pg/kg) in Untreated and Fortified Soils
Soil Type Alfisol
Sample Interval Day 0
Week 1 Week 2 Week 4
Month 2
Month 4
Month 6
Month 9
Month 12
Month 15 Month 18 Month 24
Untreated Controls Aerobic Anaerobic
<LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD CLOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD
<L0D <LOD <LOD <LOD <LOD CLOD cLOD <LOD <LOD CLOD CLOD CLOD cLOQ CLOQ
LOD = limit of detection (0.128 pg/kg) LOQ --limit of quantitation (2.0 pg/kg)
F ortified Recoveries
Aerobic
Anaerobic
9.2 8.7 9.2 10.9 9.4 8.0 7.7 9.4 10.3 9.7 7.5 8.9 8.9 9.3 8.6
12.2
8.7 8.8
92% 87% 92% 109% 94% 80% 77% 94% 103% 97% 73% 89% 89% 93% 86% 122% 87% 88%
OO oo
8.3 83% 6.1 61% 8.3 83%
8.6 86%
7.9 79% 7.9 79% 7.0 70% 7.6 76%
88%
9.9 99% 7.4 74% 7.2 72% 9.5 95% 6.7 67%
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-214-
p. 215 Project No.: 112E-108
Wildlife International, Ltd.
P ro ject N um bers 112E -108,109,110
Measured Concentrations of PFUA (pg/kg) in Untreated and Fortified Soils
Soil Type
In c e D tiso l
Sample Interval
Untreated Controls Aerobic Anaerobic
Day 0 Week 1 Week 2 Week 4 Month2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 ,
<L0Q <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD
<LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ
LOD = limit of detection (0.128 pg/kg) LOQ = limit of quantitation (2.0 pg/kg)
Fortified Recoveries
Aerobic
Anaerobic
12.0 11.2 12.5 10.1 9.2 10.3 9.8 8.9 10.4 11.7 12.2 8.8 8.2 8.3 9.9 . 8.2 7.4 6.9
120%
112%
125% 101% 92% 103% 98% 89% 104% 117%
122%
88% 82% 83% 99% 82% 74% 69%
7.9 7.4 7.9 7.4 7.4 8.7 8.0 8.5 8.7 10.3 6.9 7.3 7.0 8.5
79% 74% 79% 74% 74% 87% 80% 85% 87% 103% 69% 73% 70% 85%
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-215-
p. 216 Project No.: 112E-108
Wildlife International, Ltd.
P ro je c t N u m b e rs 112E-108.109.no
Measured Concentrations of 8-2 FTOH Qig/kg) in Untreated and Fortified Soils
Sample
Untreated Controls
Soil Type________ Interval______ Aerobic Anaerobic
Fortified Recoveries Aerobic______ Anaerobic
Ultisol
DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24
<LOD <LOD <LOD CLOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <L0D <LOD <LOD <LOD <LOD <LOD CLOD <LOD
<LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD
289 115%
283 113%
244 97%
247 99%
210
84%
208 83% 165 66%
147 59%
1 0 2 41%
( 98 1 39%
( 45 > 18%
( 53 ) 21%
( 41 ) 16%
<LOD 0% <LOD 0%
<LOD 0%
cLOD 0%
CLOD 0%
291 116% 288 115% 220 88%
258 103%
249 181 179 155 165 117 ( 87 ) ( 54 ) ( 68 )
99% 72% 71% 62% 66% 47% 35% 22% 27%
LOD = limit of detection (22.3 pg/kg) LOQ = limit of quantitation (100 pg/kg) Values in parentheses are <LOQ and >=LOD
M onday, O ctober IS , 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 216-
p. 217 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers E- ,1 1 2 1 0 8 1 0 9 ,1 1 0
Measured Concentrations of 8-2 FTOH (pg/kg) in Untreated and Fortified Soils
Soil Type Moliisol
Sample Interval
DayO
Week 1
Week 2
Week 4
Month 2
Month 4
Month6
Month 9
Month 12
Month 15 Month 18 Month 24
Untreated Controls Aerobic Anaerobic
Fortified Recoveries
Aerobic
Anaerobic
<LOD <LOD CLOD <LOD <L0D <LOD <LOD <LOD <L0D <LOD <LOD <LOD <LOD <L0D <L0D <LOD <L0D CLOD <L0D <LOD CLOD
<LOD <LOD <LOD <LOD <LOD <LOD <LOD CLOD <LOD <LOD <LOD <LOD <LOD <LOD
299 120%
261 104% 213 85% 217 87% 194 78% 112 45% 119 48% ( 51 ) 21% ( 63 ) 25% <LOD 0% ( 32 ) 13% <LOD 0% -CLOD 0% <LOD 0% <LOD 0% -CLOD 0% CLOD 0%
266 106% 279 112% 244 98% 273 109% 257 . 103% 243 97% 191 77% 192 77% ( 99 ) 40% 162 65% ( 70 ) 28% ( 46 ) 18% ( 36 ) 14% <LOD 0%
LOD - limit of detection (22.3 ng/kg) LOQ = limit of quantitation (100 pg/kg) Values in parentheses are <LOQ and >=LOD
M onday, O ctober 15, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-217-
p. 218 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers 112E-108,109,110
Measured Concentrations of 8-2 FTOH (p.g/kg) in Untreated and Fortified Soils
Sample
Untreated Controls
Fortified Recoveries
Soil Type________ Interval______ Aerobic Anaerobic_____ Aerobic______ Anaerobic
Alfisoi
D ayO
Week 1 Week 2 Week 4 Month 2 Month 4
Month Month 9 Month 12 Month 15 Month 18 Month 24
CLOD <LOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD
CLOD CLOD CLOD cLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD cLOD CLOD
307 123% 245 98% 210 84% 143 57% 156 6 2 % 142 57% 117 47% 112 45% ( 75 ) 30% ( 79 ) 32% ( 54 ) 22% ( 51 ) 21% CLOD 0% ( 29 ) 12% CLOD 0% cLOD 0% cLOD 0% CLOD 0%
267 107% 254 102% 215 86% 203 81% 205 82% 161 64% 127 51% 143 57% ( 81 1 33% ( 62 1 25% ( 50 ) 20% ( 37 ) 15% cLOD 0% CLOD 0%
LOD = limit of detection (22.3 pg/kg) LOQ = limit of quantitation (100 pg/kg) Values in parentheses are <LOQ and >=LOD
M onday, October 15, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-218 -
p. 219 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers 112E-108,109,110
Measured Concentrations of 8-2 FTOH (jig/kg) in Untreated and Fortified Soils
Soil Type Inceotisol
.
Sample Interval Day & Week 1 Week 2
Week 4
Month 2
Month 4
Month6
Month 9
Month 12
Month 15 Month 18 Month 24
Untreated Controls Aerobic Anaerobic
F ortified Recoveries
Aerobic
Anaerobic
CLOD <LOD <LOD <LOD <LOD <LOD CLOD CLOD CLOD CLOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD
CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD
285 11494 262 305% 267 10794 285 11494 245 9894 234 94% 218 87% 284 114% 201 81% 200 80% 157 6394 186 7496 ( 86 1 34% ( 85 ) 34% ( 55 ) 22% 115 46% CLOD 0% CLOD 0%
287 115% 285 114% 258 103% 260 104% 237 95% 235 94% 230 92% 219 87% 134 54% 165 66% 106 42% 106 42% ( 42 ) 17% ( 37 ) 15%
LOD = limit ofdetection (22.3 pg/kg) LOQ = limit of quantitation (100 pg/kg) Values in parentheses are <LOQ and >=LOD
M onday, O ctober 15, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-219-
p. 220 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers 112E-108.109.110
Measured Concentrations of 8-2 FTOH (pg/L) in Headspace Gases from Untreated and Fortified Soils
Sample
Untreated Controls
Fortified Soils
Soil Type________ Interval______ Aerobic Anaerobic_____ Aerobic Anaerobic
Ultisol
Day 0 Week 1 Week2 Week 4
Month 2 Month 4 Month 6 Month 9 Month 12
Month 15 Month IS Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOQ ~ limit of quantitation (12.5 pg/L of headspace)
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
M onday, O ctober 15, 2007
Page 1 o f4
. Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 220-
p. 221 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers 112E-108,109,110
Measured Concentrations of 8-2 FTOH (fig/L) in Headspace Gases from Untreated and Fortified Soils
Sample
Untreated Controls
Fortified Soils
Soil Type________ Interval______ Aerobic Anaerobic_____ Aerobic Anaerobic
M ollisol
Day 0 Week 1 Week 2 Week4 Month 2 Month 4 Month 6 Month 9
Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOQ = limit of quantitation (12.5 pg/L ofheadspace)
,M onday O ctober IS , 2007
Page 2 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-221 -
p. 222 Project No.: 112E-108
Wildlife International, Ltd.
P roject N u m b ers 1 12E -108,109,110
Measured Concentrations of 8-2 FTOH Qig/L) in Headspace Gases from Untreated and Fortified Soils
Sample
Untreated Controls
Fortified Soils
Soil Type________ Interval______ Aerobic Anaerobic_____ Aerobic Anaerobic
Alfisol .
Day 0 Week 1 Week 2 Week4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOQ = limit of quantitation (12.5 fig/L of headspace)
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
M onday, O ctober IS , 2007
Page 2 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-222-
p. 223 Project No.: 112E-108
Wildlife International, Ltd.
P ro ject N um bers 1 1 2 E -108,109,110
Measured Concentrations of 8-2 FTOH (pg/L) in Headspace Gases from Untreated and Fortified Soils
Soil Type
Sample Interval
Untreated Controls Aerobic Anaerobic
InceD tisol
Day 0 Week 1 Week2 Week 4 Month 2 Month 4 Months Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOQ = limit of quantitation (12.5 pg/L ofheadspace)
Fortified Soils Aerobic Anaerobic
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ
,M onday, O ctober 15 2007
Page 4 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
p. 224 Project No.: 112E-108
-223Appendix II Measured Concentration o f Selected Analytes (gmol/kg Soil Dry Weight)
Company Sanitized. Does not contain TSCA CBI.
,W ildlife International Ltd.
-224-
p. 225 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFOA (ftmol/kg)
in Soils Treated with(^
D
Soil Type
Sample Interval
Aerobic Live Sterile
Anaerobic Live Sterile
Ultisol
Day 0 Week 1 Week 2 Week4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24
<LOQ <LOQ 0.043 0.046 0.077 0.088 0.140 0.148 0.295 0.362 0.651 0.903 0.909 1.065 1.176 1.349 0.888 0.831 0.821 0.876 0.992 0.794 0.884 0.795
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.005 <LOQ 0.006 <LOQ 0.005 <LOQ 0.012 <LOQ 0.019 <LOQ <LOQ <LOQ
<LOQ <LOQ 0.053 0.055 0.065 0.058 0.124 0.097 0.169 0.177 0.277 0.322 0.359 0.253
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.006 <LOQ 0.007 <LOQ
LOD = limit o f detection (0.0005 pmol/kg) LOQ = limit of quantitation (0.0048 pmol/kg)
Thursday, N ovem ber 08, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-225 -
p. 226 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFOA (pmol/kg) in Soils Treated with
S am ple
A erobic
Soil Type_______Interval_________ Live Sterile
M ollisol
Day 0 W eek 1 W eek 2 Week 4 M onth 2 M onth 4 M onth 6 M onth 9 M onth 12 M onth 15 M onth IS M onth 24
<LOQ <LOQ
0.037 0.036 0.043 0 .0 4 4 0.052 0.049 0.077 0.073 0.111 0.142 0.180 0 .1 8 0 0.223 0.167 0.251 0.227 0.238 0.599 0.307 0.287 0.554 0.501
LOD = limit of detection (0.0005 pmol/kg) LOQ = limit of quantitation (0.0048 pmol/kg)
<LOQ <LOQ OLOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
0.007
<LOQ
0.008
0.006
0.008
0.008
0.007
0.008
Anaerobic Live Sterile
<LOQ <LOQ
0.035 0 .0 3 6 0.045 0.044 0.049 0 .0 5 4 0 .1 0 3 0 .1 0 4 0 .1 8 9 0 .1 4 9 0 .2 8 4 0 .2 2 0
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
0.006
<LOQ
0 .0 0 9
0 .0 0 6
Thursday, N ovem ber Od, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 226-
p. 227 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFOA (nmol/kg)
in Soils Treated with C
3
Soil Type
Sam ple Interval
Aerobic Live Sterile
Alfisol
Day 0 Week 1 Week2 Week4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
O' O' OO
0.020 0.020 0.027 0.026 0.036 0.038 0.056 0.060 0.077 0.080 0.141 0.127 0.236 0.226 0.158 0.203 0.321 0.250 0.469 0.376 0.475 0.460
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ 0.008 <LOQ <LOQ <LOQ
LOD = limit o f detection (0.0005 pmol/kg) LOQ = limit o f quantitation (0.0048 pmol/kg)
Anaerobic Live Sterile
<LOQ <LOQ 0.023 0.026 0.034 0.037 0.048 0.048 0.043 0.075 0.217 0.188 0.240 0.202
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.005 <LOQ 0.005 <LOQ
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-227-
p. 228 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFOA (jrmol/kg)
in Soils Treated with Q.
3
Soil Type
Sample Interval
Aerobic U ve Sterile
Incentisol
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 . Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ 0.046 0.050 0.069 0.070 0.135 0.132 0.295 0.236 0.460 0.457 0.639 0.605 0.697 0.820 0.888 0.845 0.972 0.930 1.020 1.078 1.282 1.338
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.005 <LOQ 0.007 <LOQ 0.011 <LOQ 0.013 0.007 0.015 0.007 0.010 0.006
LOD = limit o f detection (0.0005 nmol/kg) LOQ = limit o f quantitation (0.0048 pmol/kg)
Anaerobic Live Sterile
<LOQ <LOQ 0.050 0.043 0.064 0.065 0.182 0.116 0.333 0.349 0.687 0.731 0.790 0.838
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.012 <LOQ 0.018 <LOQ
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 228-
p. 229 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 8-2 FTUA (innol/kg)
in Soils Treated with
.
Soil Type
Sample Interval
Aerobic U ve Sterile
Ultisol
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOD <LOQ <LOQ <LOQ 0.005 0.006 0.009 0.007 0.014 0.015 0.027 0.028 0.026 0.042 0.067 0.043 0.013 0.017 0.025 0.029 0.034 0.014 0.034 0.071
LOD --limit of detection (0.0003 (imol/kg) LOQ = limit of quantitation (0.0044 pmol/kg)
<LOD <LOD <LOD <LOD <LOD <LOD CLOD <LOD <LOD <LOD <LOD <LOD CLOD
cLOD
CLOD CLOD CLOD
CLOD
Anaerobic Live Sterile
CLOD CLOD
cLOD cLOD cLOQ
CLOQ
cL O Q
CLOQ
cLOQ cLOQ
CLOQ CLOQ
cLOQ CLOQ
CLOD CLOD CLOD
clod
CLOD CLOD cLOD CLOD CLOD
cLOD
cL O D
CLOD CLOD CLOD
/
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-229-
p. 230 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 8-2 FTUA (junol/kg)
in Soils Treated with
j
Soil Type
Sam ple Interval
Aerobic Live Sterile
Moflisol -
Day 0 Week 1 Week2 Week 4 Month. 2 Month.4 Month. 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ <LOD <LOD cLOD cLOD cLOQ <LOQ cL O Q <LOQ cLOQ cLOQ <LOD <LOQ cLOQ cLOQ <LOQ cLOQ CLOD <LOD CLOQ <LOQ
0.007
CLOQ
LOD = limit o f detection (0.0003 pmol/kg) LOQ = limit o f quantitation (0.0044 pmol/kg)
cLOD cLOD cLOD CLOD cLOD <LOD cL O D cLOD cLOD CLOD <LOD CLOD cL O D CLOD CLOQ <LOQ cL O D CLOD
Anaerobic Live Sterile
cLOQ CLOQ cL O Q CLOQ cLOD CLOD cLOD CLOQ cL O Q cLOQ CLOQ cLOQ cLOQ CLOQ
cLOD CLOD cL O Q CLOD cL O D CLOD CLOD CLOD cLOD CLOD CLOD CLOQ CLOD CLOD
F riday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-230-
p. 231 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 8-2 FTUA (fimol/kg) in Soils Treated with
Soil Type
Sample Interval
Aerobic Live Sterile
Alfisol
DayO Week 1 Week 2 Week 4 Month. 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24
<LOD <LOD <LOD CLOD CLOD CLOD CLOQ CLOQ CLOQ CLOD CLOD CLOD CLOQ CLOQ CLOQ CLOQ CLOQ CLOQ CLOQ CLOQ CLOQ cLOQ cLOQ cLOQ
LOD = limit of detection (0.0003 pmol/kg) LOQ = limit o f quantitation (0.0044 pmol/kg)
CLOD CLOD cLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD <LOD CLOD CLOD CLOD CLOQ cLOQ
Anaerobic Live Sterile
CLOD CLOD CLOD CLOD CLOD cLOD cLOQ cLOQ cLOD CLOQ
0.008 0.005 0.005
cLOQ
CLOD cLOD CLOD cL O D cL O D CLOD CLOD <LOD CLOD CLOD cLOD CLOD CLOQ CLOQ
,Friday October 12, 2607
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-231 -
p. 232 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 8-2 FTUA (punol/kg)
in Soils Treated with^
3
Soil Type
Sample Interval
Aerobic Live Sterile
Incentisol
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ <LOD <LOD <LOQ <LOQ 0.013 0.011 0.055 0.027 0.170 0.194 0.358 0.350 0.419 0.352 0.457 0.452 0.492 0.550 0.559 0.530 0.542 0.522
LOD = lirait of detection (0.0003 pmol/kg) LOQ = limit of quantitation (0.0044 pmol/kg)
<LOD CLOD CLOD cLOD cLOD cLOD <LOD cLOD CLOD CLOD <LOQ cLOD 0.006 CLOQ 0.005 CLOQ CLOQ CLOQ
Anaerobic Live Sterile
CLOD CLOD cLOD cLOD CLOD cLOQ 0.017 CLOQ 0.012 0.012 0.028 0.028 0.034 0.022
cLOD CLOD CLOD CLOD <LOD <LOD cLOD <LOD cLOD CLOD cLOD CLOD <LOQ cLOQ
F riday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-232 -
p. 233 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 8-2 FTA (junol/kg) in Soils Treated wititQ 3
Sample
Aerobic
Anaerobic
S o il Type______ Interval_________ L ive Sterile_____L ive Sterile
Ultisol
DayO Week 1 Week2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOD <LOD <LOQ <LOQ 0.008 0.008 0.013 0.010 0.018 0.018 0.020 0.017 0.021 0.040 0.041 0.025 0.006 0.009 0.009 0.008 0.014 0.006 0.010 0.011
<LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD 0.004
<LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD
<LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD CLOD CLOD <LOD CLOD
LOD = limit of detection (0.0011 pmol/kg) LOQ = limit o f quantitation (0.0042 pmol/kg)
Friday, O ctober 12,2 0 0 7
Company Sanitized. Does not contain TSC'A CBI.
W ildlife International, Ltd.
-233 -
p. 234 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
M easured Concentrations o f 8-2 F T A (ju n o l/kg )
in Soils T reated w ith Q
J
Soil Type
Sample Interval
Aerobic Live Sterile
Mollisol
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
CLOD <LOQ cLOQ CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD cL O D CLOD CLOD cL O D cLOQ
0.005
cL O D CLOD CLOD cLOD cL O Q CLOQ
LOD = limit o f detection (0.0011 pmol/kg) LOQ = limit o f quantitation (0.0042 pmol/kg)
cL O D CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD cLOD CLOD CLOD <LOD <LOD <LOD
CLOD
Anaerobic U ve Sterile
CLOD CLOD cL O D CLOD CLOD cLOD CLOD cLOD cL O D CLOD CLOD cLOD CLOD CLOD
CLOD CLOD CLOD <LOD CLOD CLOD cLOD CLOD CLOD cLOD CLOD cL O D CLOD CLOD
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
,W ildlife International Ltd.
-234-
p. 235 Project No.: 112E-108
Wildlife InternationalLtd.
Project Number 112E-108
M easured Concentrations o f 8-2 F T A (fim ol/kg)
in Soils Treated w ith
1
Soil Type
Sample Interval
Aerobic Live Sterile
Anaerobic Live Sterile
Alfisol
Day 0 Week 1 Week2 Week4 Mouth2 Monti 4 Month 6 Month? Month 12 Month 15 Month 18 Month 24
CLOD CLOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD cLOQ CLOQ
LOD = limit o f detection (0.0011 pmol/kg) LOQ = limit o f quantitation (0.0042 pmol/kg)
CLOD CLOD CLOD CLOD cL O D CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD
CLOD cLOD CLOD CLOD CLOD CLOD cLOQ CLOQ CLOD CLOD CLOQ CLOQ cLOQ CLOQ
cL O D CLOD CLOD CLOD cLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOQ
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 235 -
p. 236 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
M easured C oncentrations o f 8-2 F T A (pm ol/kg)
in Soils T re ated w ith ^
3
Sample
Aerobic
Anaerobic
Soil Type________ Interval____________Live Sterile______ Live Sterile
InceDtisoI
Day 0 Week 1 Week 2 Week4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24
<LOQ <LOQ <LOD <LOD <LOQ <LOQ 0.011 0.010 0.038 0.027 0.154 0.158 0.341 0.302 0.343 0.265 0.279 0.294 0.404 0.430 0.447 0.474 0.423 0.464
LOD = limit o f detection (0.0011 pmol/kg) LOQ = limit o f quantitation (0.0042 pmol/kg)
<LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD 0.006 <LOQ 0.006 <LOQ <LOQ <LOQ
<LOD <LOD <LOQ <LOD <LOD <LOD 0.014 <LOQ 0.005 0.005 0.013 0.012 0.018 0.011
<LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD CLOD <LOQ
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-236-
p. 237 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
M easured Concentrations o f P F N A (pm ol/kg) in Soils Treated
Soil Type
Sample Interval
Aerobic Live Sterile
Ultisol
DayO Week 1 Week2 Week 4 Month2 Month4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ 0.015 0.016 0.025 0.026 0.038 0.036 0.052 0.046 0.060 0.065 0.061 0.066 0.072 0.065 0.018 0.034 0.051 0.057 0.064 0.053 0.067 0.064
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOD = limit o f detection (0.0003 pmol/kg) LOQ = limit o f quantitation (0.0043 pmol/kg)
Anaerobic Live Sterile
<LOQ <LOQ 0.014 0.015 0.020 0.020 0.034 0.029 0.033 0.046 0.050 0.048 0.065 0.055
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ
,Friday O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-237-
p. 238 Project No.: 112E-108
Wildtife International, Ltd.
Project Number 112E-108
M easured Concentrations o f P F N A (fun o l/kg )
in Soils T reated w ith
3
Soil Type
Sample Interval
Aerobic Live Sterile
Mollisol '
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month6 Month 9 Month 12 Month IS Month 18 Month 24
<LOQ
cLOQ
0.013 0.013 0.012 0.018 0.023 0.026 0.039 0.037 0.048 0.048 0.046 0.043 0.037 0.024 0.042 0.040 0.031 0.056 0.045 0.037 0.055 0.075
<LOD <LOD cL O Q CLOQ CLOD cLOD CLOQ cL O Q cLOQ <LOQ CLOQ <LOQ CLOQ cLOQ CLOQ CLOQ cL O Q cLOQ
LOD = limit o f detection (0.0003 jimol/kg) LOQ = limit o f quantitation (0.0043 pmol/kg)
Anaerobic Live Sterile
CLOQ
cLOQ
0.009 0.009 0.013 0.010 0.009 0.013 0.014 0.018 0.026 0.020 0.034 0.026
cL O D cL O D cLOQ CLOQ cL O Q cLOD cLOQ cLOQ cL O Q cL O Q cL O Q cL O Q CLOQ CLOQ
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-238 -
p. 239 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
M easured Concentrations o f P F N A (junol/kg)
in Soils T reated w ith
3
Soil Type
Sample Interval
Aerobic Live Sterile
Anaerobic Live Sterile
Alfisol .
Day 0 Week 1 Week2 Week4 Month. 2 Month 4 Month 6 Month 9 Month 12 Month 13 Month 18 Month 24
<LOQ <LOQ 0.007 0.009 0.010 0.008 0.014 0.017 0.022 0.035 0.023 0.029 0.019 0.034 0.038 0.050 0.029 0.038 0.050 0.033 0.034 0.038 0.054 0.047
<LOD <LOD <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ . <LOQ
<LOQ <LOD <LOQ <LOQ
<LOQ <LOQ <LOQ 0.005 0.006 0.007 0.010 0.010 0.008 0.014 0.019 0.020 0.024 0.017
<LOQ <LOQ <LOQ <LOD <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOD = limit o f detection (0.0003 pmol/kg) LOQ = limit o f quantitation (0.0043 pmol/kg)
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-239 -
p. 240 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
M easured Concentrations o f P F N A (junol/kg) in Soils T reated w ith
Soil Type
Sample Interval
Aerobic Live Sterile
Inceotisol
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Monlh6 . Month 9 Month 12 Month IS Month 1$ Month 24
<LOQ <LOQ 0.044 0.054 0.062 0.072 0.079 0.076 0.134 0.138 0.128 0.133 0.146 0.172 0.172 0.171 0.154 0.152 0.159 0.160 0.171 0.174 0.208 0.224
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOD = limit o f detection (0.0003 pmol/kg) LOQ = limit o f quantitation (0.0043 pmol/kg)
Anaerobic Live Sterile
<LOQ <LOQ 0.021 0.016 0.028 0.029 0.075 0.047 0.053 0.057 0.062 0.077 0.073 0.078
<LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
Friday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-240-
p. 241 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
M easured Concentrations o f P F D A (fim ol/kg )
in Soils T reated w ith
'J
Sample
Aerobic
Anaerobic
Soil Type________ Interval__________ Live Sterile______ Live Sterile
Ultisol .
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month. 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ 0.009 0.009 0.015 0.016 0.021 0.023 0.035 0.036 0.052 0.055 0.059 0.069 0.074 0.076 0.018 0.041 0.065 0.066 0.093 0.071 0.099 0.086
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOD = limit o f detection (0.0002 pmol/kg) LOQ = limit of quantitation (0.0039 pmol/kg)
<LOQ <LOQ 0.009 0.010 0.013 0.014 0.018 0.016 0.026 0.029 0.049 0.055 0.043 0.038
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
Thursday, N ovem ber OS, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-241 -
p. 242 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFDA (funol/kg)
in Soils Treated with^
3
Soil Type
Sample Interval
Aerobic Live Sterile
Anaerobic Live Sterile
Mollisol '
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ 0.007 0.007 0.009 0.010 0.012 0.012 0.019 0.020 0.028 0.032 0.034 0.034 0.032 0.024 0.038 0.036 0.028 0.054 0.043 0.042 0.088 0.094
LOD = limit o f detection (0.0002 pmol/kg) LOQ = limit o f quantitation (0.0039 pmol/kg)
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.004 <LOQ <LOQ <LOQ <LOQ 0.004 <LOQ <LOQ
<LOQ <LOQ 0.007 0.007 0.010 0.009 0.013 0.013 0.021 0.021 0.034 0.028 0.041 0.034
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
Thursday, N ovem ber 08, 2007
Company Sanitized. Does not contain TSCA CB1.
,W ildlife International Ltd.
-242-
p. 243 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFDA (pmol/kg) in Soils Treated withQ
Sample
Aerobic
Anaerobic
Soil Type________ Interval___________ Live Sterile______ Live Sterile
Alfisol
DayO Weekl Week 2 Week4 Month 2 Month 4 MODth6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ <LOQ 0.004 0.006 0.006 0.009 0.009 0.016 0.018 0.016 0.019 0.018 0.024 0.033 0.038 0.025 0.031 0.051 0.030 0.045 0.040 0.073 0.071
LOD = limit of detection (0.0002 pmol/kg) LOQ = limit o f quantitation (0.0039 pmol/kg)
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ
<LOQ <LOQ <LOQ 0.005 0.006 0.007 0.011 0.011 0.009 0.017 0.025 0.026 0.028 0.024
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
Friday. O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-243 -
p. 244 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFDA (fimol/kg)
in Soils Treated with
J
Soil Type
Sample Interval
Aerobic Live Sterile
InceDtisol
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ 0.009 0.010 0.016 0.016 0.030 0.030 0.067 0.058 0.085 0.098 0.102 0.134 0.130 0.111 0.121 0.136 0.122 0.128 0.158 0.151 0.169 0.174
<LOQ <LOQ <120Q <LOQ <LOQ <LOQ <LOQ <LOQ 0.004 <LOQ 0.005 <LOQ 0.006 0.006 0.006 0.005 0.004 0.004
LOD = limit o f detection (0.0002 pmol/kg) LOQ = limit o f quantitation (0.0039 pmol/kg)
Anaerobic Live Sterile
<LOQ <LOQ 0.010 0.009 0.015 0.015 0.041 0.022 0.042 0.045 0.065 0.076 0.091 0.080
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.005 <LOQ
F riday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
Wildlife International, Ltd.
-244-
p. 245 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFUA Qunol/kg) in Soils Treated with
Soil Type
Sample Interval
Aerobic Live Sterile
Dltisoi .
DayO Week 1 Week2 Week 4 Month2 Month. 4 Month6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ <LOQ <LOQ 0.005 0.006 0.009 0.008 0.015 0.014 0.023 0.027 0.028 0.029 0.033 0.030 0.006 0.019 0.027 0.028 0.034 0.029 0.040 0.040
LOD = limit o f detection (0.0002 pmol/kg) LOQ = limit o f quantitation (0.0036 pmol/kg)
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
Anaerobic Live Sterile
<LOQ <LOQ <LOQ <LOQ 0.005 0.005 0.007 0.007 0.012 0.015 0.027 0.023 0.032 0.031
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
'
Friday, O ctober 12, 2907
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-245 -
p. 246 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFUA Qunol/kg) in Soils Treated withC 3
Sample
Aerobic
Anaerobic
Soil Type________ Interval___________ Live Sterile______ Live Sterile
Mollisol
DayO Week 1 Week2 Week4 Month.2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ 0.004 0.005 0.005 0.011 0.010 0.018 0.019 0.017 0.017 0.015 0.008 0.016 0.015 0.011 0.020 0.018 0.016 0.026 0.038
LOD - limit ofdetection (0.0002 pmoldsg) LOQ = limit ofquantitation (0.0036 pmol/kg)
<LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOD <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.004 0.004 0.006 0.011 0.008 0.012 0.010
<LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOD
Friday. O ctober 22, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-246-
p. 247 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFUA (junol/kg)
in Soils Treated with(_
J
Sample
Aerobic
Anaerobic
Soil Type________ Interval___________ Live Sterile______ Live Sterile
Alfisol
Day 0
Week 1 . ' Week2
Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOD <LOD
<LOQ <LOQ <LOQ
<LOQ
0.004 0.004 0.008
0.011
0.007 0.011 0.006 0.011 0.013
0.020
0.014 0.016 0.024
0.010
0.012
0.013 0.025 0.018
LOD = limit o f detection (0.0002 pmol/kg) LOQ = limit o f quantitation (0.0036 pmol/kg)
<LOD <LOD <LOQ <LOQ <LOD <LOD cLOD CLOD CLOD CLOD cL O Q CLOD cLOD cLOQ cL O Q cL O D cLOQ CLOQ
CLOD CLOD
CLOQ cLOQ cL O Q CLOQ cLOQ cL O Q cL O Q
0.005 0.005 0.006 0.008 0.006
cLOD CLOD cLOD cLOD CLOQ CLOQ cLOD CLOD cL O Q CLOD CLOD CLOD cL O Q cL O Q
Friday. O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-247-
p. 248 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of PFUA (jimol/kg)
in Soils Treated w ith
J
Sample
Aerobic
Anaerobic
Soil Type________ Interval___________ Live Sterile______ Live Sterile
Inceotisol
Day 0 Week 1 Week 2 Week4 Month 2 Month 4 Monlh6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ <LOQ 0.004 0.007 0.008 0.013 0.011 0.034 0.035 0.033 0.033 0.037 0.050 0.052 0.050 0.046 0.052 0.046 0.045 0.062 0.058 0.072 0.080
LOD = limit o f detection (0.0002 pmol/kg) LOQ = limit o f quantitation (0.0036 pmol/kg)
<LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOD <LOD <LOQ <LOQ 0.006 0.006 0.017 0.011 0.025 0.025 0.030 0.034 0.039 0.037
<LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOD <LOQ <LOQ
F riday, O ctober 12, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-248 -
p. 249 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 8-2 FTOH (junol/kg)
in Soils Treated with
3
Soil Type
Sample Interval
Aerobic Live Sterile
Ultisol
Day 0 Week I . Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOD <LOD
<LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD CLOD <LOD
LOD --limit o f detection (01048 pmol/kg) LOQ = limit o f quantitation. (0.216 pmol/kg)
Anaerobic Live Sterile
<LOD <LOD <LOD <LOD
<LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD
<LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD
M onday, O ctober IS , 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-249-
p. 250 Project No.: 112E-108
Wildlife International, Ltd,
Project Number 112E-108
Measured Concentrations of 8-2 FTOH (pmol/kg)
in Soils Treated withU
3
Soil Type
Sample Interval
Aerobic Live Sterile
Mollisol
Day 0 Week 1 Week2 Week 4 Month 2 Month4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOD <LOD cL O D <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD CLOD cL O D CLOD CLOD CLOD <LOD cL O D CLOD cL O Q CLOD CLOD
LOD - limit o f detection (0.048 pmol/kg) LOQ = limit o f quantitation (0.216 uniol/kg)
CLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD <LOD CLOD cLOD CLOD
Anaerobic Live Sterile
CLOD cL O D cLOQ CLOQ CLOD CLOD cL O D CLOD cLOD CLOD cLOD CLOD cLOD cLOD
CLOD cL O D cL O D CLOD cL O D CLOD cLOD cL O D CLOD cL O D cL O D CLOD cLOD CLOD
M onday, O ctober IS , 2007
Company Sanitized. Does not contain TSCA CBI.
,W ildlife International Ltd.
-250-
p. 251 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 8-2 FTOH (pmol/kg)
in Soils Treated withQ
D
Soil Type
Sample Interval
Aerobic Live Sterile
Anaerobic Live Sterile
Alfisol
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month L2 Month 15 Month 18 Month 24
<LOD CLOD <LOD <LOD <LOD <LOD <LOD <LOD CLOD CLOD cLOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD cLOD cLOD CLOD CLOD cLOQ CLOD
LOD = limit o f detection (0.048 pmol/kg) LOQ = limit o f quantitation (0.216 pmol/kg)
CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD cL O D CLOD <LOD cLOD cLOD CLOD
CLOD
CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOQ CLOD cLOD CLOD
CLOD CLOD CLOD cLOD CLOD CLOD cLOD CLOD <LOD CLOD cL O D CLOD cL O D cL O D
M onday, O ctober IS , 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-251 -
p. 252 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 8-2 FTOH (junol/kg)
in Soils Treated with(.
T
Soil Type
Sample Interval
Aerobic Live Sterile
Inceotisol
Day 0 Week 1 Week 2 Week 4
'
Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOD <LOD <LOD CLOD CLOD CLOD cLOQ CLOQ
0.374 0.269 0.319 0.297
cLOQ
0.302 0.216
cLOQ
0.282 0.356 0.374 0.261
cLOQ CLOQ cL O Q cLOQ
LOD = limit o f detection (0.048 pmol/kg) LOQ = limit o f quantitation (0.216 pmol/kg)
<LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD <LOQ CLOD cLOD CLOD cL O D CLOD CLOD CLOD
Anaerobic Live Sterile
CLOD cLOD CLOD CLOD CLOD cLOD cLOQ CLOD cLOQ CLOQ cLOQ CLOQ CLOQ CLOQ
CLOD CLOD cL O D cL O D CLOD CLOD CLOD CLOD cLOQ cL O D cL O D CLOD cLOD CLOD
M onday, O ctober 15, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-252-
p. 253 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 8-2 FTOH (pmol/L) in Headspaces Gases from Chambers Treated with
Sample
Aerobic
Anaerobic
Soil Type________ Interval___________ Live Sterile______ Live Sterile
Dltisol
DayO Week 1 Week2 Week 4 Month. 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOQ = limit of quantitation (0.027 {imol/L ofheadspace)
M onday, O ctober IS , 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-253 -
p. 254 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations,of 8-2 FTOH (pmol/L) in Headspaces Gases from Chambers Treated withC
1
Soil Type
Scingile Interval
Aerobic Live Sterile
MoDisol
DayO Week 1 Week 2 Week 4 Month 2 Month4 Month6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOQ = limit of quantitation (0.027 pmol/L ofheadspace)
Anaerobic Live Sterile
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
M onday, O ctober IS , 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-254-
p. 255 Project No.: 112E-108
Wildlife International, Ltd.
P ro je ct N u m b er 112E-108
Measured Concentrations of 8-2 FTOH (pjmol/L) in Headspaces Gases from Chambers Treated w ith
1
Sample
Aerobic
Anaerobic
Soil Type________ Interval___________ Live Sterile______ Live Sterile
Alfisol
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOQ = limit o f quantitation (0.027 pmol/L ofheadspace)
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
M onday, O ctober 15, 2007
Company Sanitized. Does not contain TSCA CBI.
,W ildlife International Ltd.
-255 -
p. 256 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 8-2 FTOH (pmol/L) in Headspaces Gases from Chambers Treated withjj,
D
Soil Type
Sample Interval
Aerobic Live Sterile
InceotisoL
DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 M onth 12 M onth 15 Month IS M onth 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOQ = limit of quantitation (0.027 pmol/L ofheadspace)
Anaerobic Live Sterile
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
M onday, O ctober IS , 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-256-
p. 257 Project No.: 112E-108
Wildlife InternationalLtd.
Project Number 112E-108
Measured Concentrations of 7-2 s FTOH (junol/kg)
in Soils Treated with
3
Soil Type
Sample Interval
Aerobic Live Sterile
Ultisol
DayO Month 2 Month 6 Month 9 Month 12 Month IS Month 18 Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ 0.321 0.401 <LOQ <LOQ <LOQ <LOQ 0.279 <LOQ <LOQ <LOQ <LOQ
LOQ = limit ofquantitation (0.24 jimol/kg)
Anaerobic Live Sterile
Thursday, N ovem ber 0 8 2 0 0 7
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-257 -
p. 258 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 7-2 s FTOH Qimol/kg) in Soils Treated w ith
Sam ple
A erobic
A naerobic
S o il Type_______In terval_________ L ive S terile_____ L ive S terile
Mollisol
Day 0 M onlli2 M onth 6 M ontli9 M onth 12 M o n th 15 M onth 18 M onth 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOQ = limit of quantitation (0.24 pmol/feg) '
Thursday, N ovem ber 08, 2007
Company Sanitized. Does not contain TSCA CB1.
W ildlife International, Ltd.
-258 -
p. 259 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 7-2 s FTOH (pmol/kg) in Soils Treated with^
S oil Type
Sam ple In terval
A erobic L ive S terile
Alfisol
DayO Month 2 Month6 Month 9 Month 12 Month IS Month 18 Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOQ = limit of quantitatiaDL(0.24 pmol/kg)
A naerobic L ive S terile
Thursday, N ovem ber 08, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-259 -
p. 260 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 7-2 s FTOH Qunol/kg)
in Soils Treated with
^
Sam ple
A erobic
A naerobic
S o il Type______ In terval_________ L ive Sterile_____L ive S terile
O' O
33
Inceotisol
DayO
Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ 0.672 0.672 0.466 1.121 0.466 0.287 0.418 0.440 <LOQ <LOQ <LOQ <LOQ
LOQ = limit of quantitation (024 iimol/kg)
Thursday, N ovem ber 08, 2007
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 260-
p. 261 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 7-3 Acid (p.moI/kg)
in Soils Treated withQ
^
Sam ple
A erobic
A naerobic
S o il Type_______In terval_________ L ive Sterile_____ L ive Sterile
Ultisol
Day 0 Month2 Mondi 6 Month 9 Month 12 Month IS Month 18 Month24
<LOQ <LOQ 0.015 0.021 0.037 0.048 0.071 0.065 0.094 0.076 0.077 0.091 0.063 0.038 0.123 0.127
'
LOQ= limit of quantitation (0.0045 pmol/kg)
W ednesday, M arch 12,2008
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-261 -
p. 262 Project No.: 112E-108
Wildlife International, Ltd.
P roject N um ber 112E-108
Measured Concentrations of 7-3 Acid (pmol/kg)
in Soils Treated with
^
Soil Type
Sample Interval
Aerobic Live Sterile
Mollisol
Day 0 Month 2 Month 6 Month 9 Month 12 Month IS Month 18 Month 24
<LOQ <LOQ <LOQ <LOQ 0.005 0.006 0.006 0.006 0.011 0.011 0.015 0.020 0.008 0.008 0.020 0.015
LOQ= limit of quantitation (0.0045 pmol/kg)
Anaerobic Live Sterile
W ednesday, M arch 12,2008
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
- 262-
p. 263 Project No.: 112E-108
Wildlife International, Ltd.
Project Number 112E-108
Measured Concentrations of 7-3 Acid (fimol/kg)
in Soils Treated w ith
3
S o il Type
Sam ple In terval
A erobic l iv e S terile
A lfisol
Day 0 M onth 2
Month 6 Month9 Month 12 Mon* 15 Month 18 Month 24
<LOQ
<LOQ <LOQ
<LOQ
0.007 0.006 0.007 0.006 0.011 0.014 0.017 0.020 0.018 0.015 0.015 0.016
LOQ= limit of quantitation (0.0045 pmol/kg)
A naerobic L ive S terile
W ednesday, M arch 12,2008
I
1
Company Sanitized. Does not contain TSCA CB1.
W ildlife International, Ltd.
-263 -
p. 264 Project No.: 112E-108
Wildlife International, Ltd.
Project Num ber 112E-108
Measured Concentrations of 7-3 Acid (pmol/kg)
in Soils Treated with.
3
Sample Soil Type________ Interval
A erobic L ive S terile
A naerobic L ive S terile
Inceotiso)
Day 0
0o
tO o
Month2 Month 6 Month 9 Month 12 Month 15 Month IS Month24
0.038 0.022 0.153 0.185 0.290 0.347 0.626 0.557 0.590 0.655 0.638 0.480 0.803 0.728
LOQ= limit of quantitation (0.0045 pmol/kg)
W ednesday, M arch 12, 2008
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-264-
p. 265 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers 1 1 2 E-1 0 8 , 109,110
Measured Concentrations of PFOA (pjmoi/kg) in Untreated and Fortified Soils
S o il Type
Sam ple In terval
U ntreated C ontrols A erobic A naerobic
Ultisol
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6
Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOD = limit of d&ection (0.0005 p.mol/kg) LOQ = limit of quantitation (0.0048 jimol/kg)
F ortified R ecoveries
A erobic
A naerobic
0.023 0.019 0.021 0.022 0.022 0.022 0.025 0.025 0,029 0.028 0.026 0.025 0.022 0.024 0.018 0.017
95% 78% 85% 91% 92% 92% 104% 103% 120% 114% 106% 104% 92% 100% 74% 68%
0.023 0.022 0.025 0.027 0.022 0.024 0.023 0.022 0.027 0.026 0.027 0.027 0.027 0.028
95% 92% 105% 113% 92% 98% 93% 90% 114% 108% 114% 113% 112% 115%
Friday, O ctober 12, 2007
Page 1 o f4
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W ildlife International, Ltd.
-265 -
p. 266 Project No.: 112E-108
Wildlife International, Ltd.
P ro je ct N u m b ers 1 12E -108,109;1 10
M easured Concentrations o f P F O A (pm ol/kg) in U ntreated and F o rtifie d Soils
Sample
Untreated Controls
F ortified Recoveries
Soil Type________ Interval______ Aerobic Anaerobic_____ Aerobic______ Anaerobic
Mollisol .
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOD <LOD <LOD <LOD cLOD CLOD cLOQ CLOQ cLOD CLOD CLOQ CLOD CLOQ cLOQ CLOQ CLOD cLOD CLOD cL O D cLOD CLOQ
cLOD CLOD CLOD cLOQ cLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD
0.022 0.022 0.023 0.024 0.021 0.023 0.022 0.022 0.026 0.027 0.032 0.029 0.032 0.029 0.028 0.028 0.022 0.025
89% 93% 97% 100%
88%
94% 91% 90% 107% 112% 132% 121% 131% 120% 114% 116% 92% 104%
0.023 0.024 0.020 0.023 0.020 0.021 0.023 0.024 0.030 0.026 0.034 0.034 0.035 0.035
93% 101% 85% 96% 85% 89% 95% 99% 122% 109% 140% 139% 145% 143%
LOD " limit of detection (0.0005 umoi/kg) LOQ = limit of quantitation (0.0048 pmol/kg)
Friday, O ctober 12, 2007
Page 2 o f4
Company Sanitized. Does not contain TSCA CB1.
W ildlife International, Ltd.
- 266-
p. 267 Project No.: 112E-108
Wildlife International, Ltd.
ProjectNumbersll2E-108,109,110
M easured Concentrations o f P F O A (pm ol/kg) in U ntreated and F o rtifie d Soils
Sample
Untreated Controls
Fortified Recoveries
Soil Type________ Interval______ Aerobic Anaerobic_____ Aerobic______ Anaerobic
Alfisol
Day 0 Week 1 Week 2 Week4 Month2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOD <LOD <LOD CLOD <LOD CLOD CLOD CLOD CLOD CLOD CLOQ <LOQ CLOD CLOD cLOD CLOD CLOD CLOD cLOD CLOD CLOD
LO D --limit of detection (0.0005 pmol/kg) LO Q = limit ofquantitation (0.0048 pmol/kg)
CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD
0.022 0.020 0.022 0.024 0.024 0.023 0.024 0.024 0.026 0.026 0.026 0.028 0.027 0.027 0.028 0.030 0.022 0.022
92% 83% 89% 100% 99% 94% 100% 101% 110% 107% 108% 115% 110% 111% 113% 125% 92% 93%
0.022 0.020 0.021 0.022 0.023 0.024 0.025 0.025 0.025 0.026 0.027 0.026 0.025 0.027
92% 82% 87% 93% 96% 100% 106% 104% 104% 107% 111% 109% 105% 112%
Friday, O ctober 12, 2607
Page 3 o f4
p. 268
Company Sanitized. Does not contain TSCA CBI.
________W ildlife International, Ltd.
project no - 112E-108
-267-
WVViMldWllitfjCe Ifnibteerrnrua+MtiUoJnt*a*'l*,, Ltd. ____________ Project Numbers 112E-108,'109,110
M easured Concentrations o f P F O A (pm ol/kg) in U ntreated and F o rtifie d Soils
Soil Type
Sample Interval
Untreated Controls Aerobic Anaerobic
Inceotisol
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4
Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOD = limit of detection (0.0005 pmol/kg) LOQ = limit of quantitation (0.0048 pmol/kg)
Fortified Recoveries
Aerobic
Anaerobic
0.025 104,4 0.025 lo:% 0.031 129,4 0.024 101% 0.026 106,4 0.026 109,4 0.030* 124,4 0.027 112,4 0.027 113,4 0.028 117,4 0.032 134,4 0.026 108,4 0.029 121,4 0.026 106,4 0.035 145,4 0.030 124,4 0.023 96,4 0.023 96,4
0.023 0.023 0.026 0.024 0.026 0.025 0.026 0.026 0.029 0.027 0.026 0.028 0.027 0.028
97% 94% 109% 98% 108% 105% 107% 109% 119% 111% 106% 115% 113%
116%
Friday, O ctober 12, 2007
Page 4 o f 4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-268-
p. 269 Project No.: 112E-108
WiIdlife International, Ltd.
Project Numbers 112E-108,109,110
Measured Concentrations of 8-2 FTUA (pmol/kg) in Untreated and Fortified Soils
Sam ple
U ntreated C ontrols
F ortified R ecoveries
S o il Type__________ In terva l________ A ero b ic A naerobic______ A erobic_______ A naerobic
U ltiso l
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month $ Month 12 M onth 15 Month IS Month 24
<LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <L0D CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD
LOD = lim it o f detection (0.0003 junol/kg) LOQ = lim it o f quantitation (0.0044 ^mol/kg)
CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD
'
0.016 0.014 0.017 0.017 0.015 0.015 0.016 0.016 0.019 0.017 0.014 0.016 0.006 0.012 0.011 0.009
72% 65% 77% 79% 69% 69% 72% 72% 89% 80% 65% 73% 29% 54% 51% 40%
0.017 0.016 0.017 0.019 0.018 0.018 0.018 0.017 0.020 0.017 0.018 0.017 0.014 0.015
80% 74% 77% 87% 84% 82% 82% 79% 93% 76% 82% 80% 65% 70%
Friday, O ctober 12 ,2 0 0 7
P age J of.4
p. 270
Company Sanitized. Does not contain TSCA CBI.
.W ild life International, L td _____________________________Project no.: m E -io s
-269-
Wildlife International, Ltd._____________ Project Numbers 112B-108,109,110
Measured Concentrations of 8-2 FTUA (pmol/kg) in Untreated and Fortified Soils
S o il Type
Sam ple In terval
U ntreated C ontrols A erobic A naerobic
M ollisol
DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 M onth 12 Month IS M onth 18 M onth 24
<LOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD cLOD cLOD
cLOD CLOD cLOQ cLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD
LO D --limit o f detection (0.0003 pjnol/kg) LO Q = lim it o f quantitation (0.0044 fimol/kg)
F ortified R ecoveries
A erobic
A naerobic
0.020 0.020 0.018 0.019 0.020 0.021 0.020 0.020 0.019 0.019 0.022 0.020 0.022 0.018 0.017 0.020 0.013 0.014
92% 90% 81% 85% 91% 95% 90% 91% 88% 87% 100% 94% 102% 82% 78% 90% 59% 63%
0.016 0.019 ,0.017 0.019 0.017 0.021 0.018 0.018 0.021 0.017 0.022 0.023 0.022 0.019
73% 88% 77% 87% 77% 98% 80% 80% 97% 78% $9% 107% 100% 85%
,Friday O ctober 12, 2007
Page2 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-270-
p. 271 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers 1 1 2 E-K)8 , 1 0 9 ,1 1 0
Measured Concentrations of 8-2 FTUA (jimol/kg) in Untreated and Fortified Soils
Sam ple
Untreated C ontrols
S o il Type__________ In terval________ A ero b ic A naerobic
F ortified R ecoveries
A erobic
A naerobic
A lfiso l
DayO Week 1 Week 2 Week 4 M onth2 Month 4 Month 6 Month 9 M onth 12 M onth IS Month 18 Month 24
<LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD
LOD = lim it o f detection (0.0003 nmol/kg) LOQ = lim it o f quantitation (0.0044 pmol/kg)
<LOD <LOD <LOD <LOD <LOD <LOD cLOD <LOD CLOD <LOD cLOD CLOD cLOD CLOD
0.018 0.017 0.019 0.021 0.023 0.017 0.016 0.020 0.021 0.020 0.014 0.020 0.018 0.020 0.018 0.024 0.014 0.015
84% 77% 86% 98% 105% 76% 75% 92% 96% 91% 66% 90% 84% 90% 80% 111% 62% 67%
0.020 0.016 0.020 0.021 0.020 0.019 0.019 0.020 0.024 0.024 0.018 0.018 0.023 0.018
89% 75% 93% 95% 92% 89% 86% 94% 309% 308% 81% 83% 103% 83%
Friday, O ctober 12, 2007
Page 2 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-271 -
p. 272 projectNo.: 112E-108
Wildlife International, Ltd.
Project Numbers 112E-108,109,110
Measured Concentrations of 8-2 FTUA (ftmol/kg) in Untreated and Fortified Soils
Soil Type
Sample Interval
Untreated Controls Aerobic Anaerobic
Inceutisol
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4
Month 6 Month 9 Month 12 Month IS Month 18 Month 24
CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD CLOD
CLOD cLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD cLOD cLOD CLOD
LOD = limit of detection (0.0003 pmol/kg) LOQ = limit of quantitation (0.0044 pmol/kg)
Fortified Recoveries
Aerobic
Anaerobic
0.024 0.021 0.024 0.018 0.019 0.021 0.020 0.017 0.018 0.020 0.022 0.018 0.015 0.015 0.016 0.014 0.012 0.011
110%
96% 108% 83%
88%
97% 94% 79% 80% 89%
101%
81% 70%
68%
74% 64% 53% 52%
0.017 0.018 0.018 0.016 0.019 0.017 0.017 0.018 0.017 0.019 0.014 0.015 0.013 0.016
79% 81% 83% 75% 85% 78% 77%
82%
76%
88% 66%
70% 61% 71%
Friday, O ctober 12, 2007
P a g e4 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-272-
p. 273 Project No.: 112E-108
Wildlife International, Ltd.
ProjectNumbersll2E-108,109,110
Measured Concentrations of 8-2 FTA (jtunol/kg) in Untreated and Fortified Soils
Sample
Untreated Controls
Fortified Recoveries
Soil Type________ Interval______ Aerobic Anaerobic_____ Aerobic______ Anaerobic
Ultisol
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOD <LOD
<LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD <LOD CLOD cLOD CLOD
CLOD cLOD cLOQ CLOQ cLOD cLOD
CLOD
LOD = limit ofdetection (0.0011 pxnol/kg) LOQ - limit of quantitation (0.0042 jimol/kg)
CLOD CLOD CLOD cLOD CLOD CLOD CLOD <LOD CLOD CLOD CLOD CLOD cLOD CLOD
0.013 0.012 0.013 0.014 0.011 0.011 0.011 0.011 0.007 0.005 0.007 0.008 cLOQ CLOQ 0.009 cLOQ
1% 58% 62% 69% 54% 55% 54% 53% 33% 24% 34% 37%
41%
0.014 0.014 0.015 0.015 0.013 0.014 0.011 0.013 0.015 0.011
0 .0 1 0
0 .0 1 0
0.007 0.007
67% 66% 70% 69% 61% 65% 53% 63% 72% 51% 48% 46% 35% 32%
Friday, October 12, 2007
Page 1 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-273 -
p. 274 project no : 112E-ios
Wildlife International, L td._________________ P ro je c t N u m b e rs 11 2 E -1 0 8 ,1 0 9 ,1 1 0
Measured Concentrations of 8-2 FTA (fimol/kg) in Untreated and Fortified Soils
S oil Type M ollisol
Sam ple In terval
DayO
Week 1 Week 2
Week 4
Month 2 M onth 4-
Month 6
Month 9
M onth 12 M onth 15 M onth 18 M onth 24
U ntreated C ontrols A erobic A naerobic
<LOD CLOD
0.006
CLOD CLOD CLOD cLOQ CLOD cLOD CLOD cLOD CLOD cLOD CLOD cLOD CLOD cLOD cLOQ cLOD CLOD cLOD
CLOD CLOD CLOD CLOD CLOD CLOD cLOD cLOD CLOD CLOD cLOD CLOD cLOD CLOD
L O D *lim it o f detection (0.0011 fimol/kg) LO Q = lim it o f quantitation (0.0042 pmol/kg)
F ortified R ecoveries
A erobic
A naerobic
0.018 0.019 0.018 0.019 0.019 0.018 0.017 0.017 0.016 0.017 0.017 0.014 0.013 0.013 0.010 0.013 0.005
CLOQ
87% 90% 84% 90% 90% 88% 83% 81% 78% 79% 84% $9% $4% 62% 50% 63% 23%
0.015 0.018 0.015 0.016 0.013 0.017 0.013 0.015 0.017 0.015 0.012 0.014 0.012 0.011
70% 87% 71% 76% 64% 83% 63% 70%
80%
71% 58%
6S%
57% 52%
Friday, O ctober 12, 2007
Page 2 o f4
Company Sanitized. Does not contain TSCA CBI
W ildlife International, Ltd.
-274-
p. 275 Project No.: 112E-108
Wildlife International, Ltd.
ProjectN um bersiiZE-108, 109,110
Measured Concentrations of 8-2 FTA (jimol/kg) in Untreated and Fortified Soils
S o il Type
Sam ple
U ntreated C ontrols
F ortified R ecoveries
In terval_________A erobic A naerobic______ A erobic
A naerobic
A liiso l
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15
Month IS
Month 24
<LOD <LOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOQ
CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOQ cLOQ
0.016 0.017 0.016 0.018 0.022 0.018 0.014 0.017 0.016 0.015 0.009 0.014 0.013 0.014 0.011 0.016 0.008 0.008
78% 79% 78% 84% 106% 87% 68% 84% 75% 70% 42% 67% 60% 66% 51% 77% 40% 37%
0.018 0.014 0.018 0.018 0.018 0.017 0.016 0.017 0.018 0.017 0.014 0.014 0.015 0.015
88% 67% 85% 84% 84% 81% 77% 83% 88% 83% 67% 66% 73% 70%
LOD = limit o f detection (0.0011 pmol/kg) LOQ = limit o f quantitation (0.0042 pm ol/kg)
Friday, O ctober 12, 2007
Page 3 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-275 -
p. 276 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers 112E-108,109,110
Measured Concentrations of 8-2 FTA (junol/kg) in Untreated and Fortified Soils
Sample
Untreated Controls
Fortified Recoveries
Soil Type________ Interval______ Aerobic Anaerobic_____ A erobic_____ Anaerobic
Inceotisol
DayO Week 1 Week 2 Week 4 Month 2 Mbnth4 Month 6
Month 9 Month 12 Month 15 Month 18 Month 24
<LOD <LOD CLOD <LOD CLOD CLOD cLOQ CLOD CLOD CLOD cLOD CLOD cLOD <LOD cLOQ cLOQ cLOQ CLOD cLOD CLOD cLOQ
LOD = limit of detection (0.0011 pmol/kg) LOQ = limit of quantitation (0.0042 pmol/kg)
CLOD CLOD CLOD CLOD CLOD CLOD CLOD <LOD cLOD cLOD cLOD CLOD cLOD CLOD
0.022 0.020 0.020 0.016 0.015 0.018 0.015 0.013 0.011 0.012 0.011 0.009 0.009 0.008 0.007 0.007 0.004 CLOQ
10794 96S 98% 76% 70% 84% 71% 63% 55% 58% 53% 45% 43% 37% 34% 34% 21%
0.016 0.015 0.015 0.014 0.015 0.016 0.012 0.014 0.010 0.013 0.008 0.007 0.005 0.007
78% 72% 70% 67% 72% 76% 59% 66%
48%
63% 36% 34% 26% 35%
Friday, O ctober 12, 2007
Page 4 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-276-
p. 277 Project No.: 112E-108
Wildlife International, Ltd.
P roject N um bers 1 12E-108,109,110
Measured Concentrations of PFNA (umol/kg) in Untreated and Fortified Soils
Soil Type
Sample Interval
Untreated Controls Aerobic Anaerobic
Ultisol
DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOD = limit of detection (0.0003 jamol/kg) LOQ = limit of quantitation (0.0043 pmol/kg)
Fortified Recoveries
Aerobic
Anaerobic
0.020 0.017 0.021 0.021 0.021 0.021 0.022 0.022 0.024 0.024 0.022 0.023 0.015 0.022 0.017 0.014
94% 78% 97% 98% 95% 98% 104%
101%
114% 114% 103% 106% 70% 103% 79% 67%
0.022 0.021 0.021 0.023 0.021 0.022 0.022 0.021 0.024 0.024 0.025 0.025 0.023 0.025
100% 99% 99% 107% 97% 103% 103% 96% 112% 110% 116% 117% 108% 116%
Friday, O ctober 12, 2007
Page 1 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-277-
p. 278 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers 112E-108,109,110
Measured Concentrations of PFNA (junol/kg) in Untreated and Fortified Soils
Soil Type
Sample Interval
Untreated Controls Aerobic Anaerobic
MoDisol
DayO Week 1 Week 2 Week 4 Month 2 Month 4
Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD
LOD = limit of detection (0.0003 pmol/kg) LOQ = limit of quantitation. (0.0043 pmol/kg)
<LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD CLOD CLOD cLOD CLOD CLOD CLOD
Fortified Recoveries
Aerobic
Anaerobic
0.020 0.021 0.021 0.021 0.021 0.021 0.022 0.018 0.023 0.023 0.026 0.026 0.026 0.025 0.022 0.024 0.017 0.018
94% 96% 97% 97% 95% 99% 102% 86% 107% 106% 122% 119% 120% 117% 100% 112% 81% 83%
0.021 0.022 0.019 0.021 0.021 0.021 0.020 0.021 0.023 0.022 0.025 0.028 0.026 0.026
96% 101% 89% 98% 96% 99% 93% 98% 107% 101% 116% 128% 122% 119%
Friday, O ctober 12, 2007
F a g e 2 o f4
Company Sanitized. Does not contain TSCA CBI.
,W ildlife International Ltd.
-278 -
p. 279 Project No.: 112E-108
Wildlife International, Ltd.
P ro ject N u m b ers 1 1 2 E -1 0 8 ,109,110
Measured Concentrations of PFNA (pmoi/kg) in Untreated and Fortified Soils
Sample
Untreated Controls
Fortified Recoveries
Soil Type________ Interval______ Aerobic Anaerobic_____ Aerobic______ Anaerobic
Aliisol
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 13 Month 18 Month 24
<LOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD
CLOD CLOD <LOD CLOD <L0Q cLOQ cLOQ CLOQ cLOQ cLOQ CLOD CLOD CLOD
CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOQ CLOQ
0.021 0.020 0.020 0.024 0.022 0.020 0.022 0.023 0.024 0.024 0.022 0.025 0.023 0.025 0.024 0.026 0.019 0.018
98% 93% 94% 112% 103% 91% 103% 107% * 113% 110% 100% 114% 108% 114% 113% 123% 87%
86%
0.021 0,017 0.020 0.022 0.021 0.022 0.022 0.022 0.024 0.024 0.023 0.023 0.025 0.022
97% 81% 94% 101% 98% 101% 103% 100% 110% 110% 107% 107% 115% 102%
LOD - limit of detection (0.0003 pmol/kg) LOQ = limit of quantitation (0.0043 pmol/kg)
,Friday October 12, 2007
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W ildlife International, Ltd.
-219-
p. 280 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers 112E-108,109,110
Measured Concentrations of PFNA (junol/kg) in Untreated and Fortified Soils
Soil Type
Sample Interval
Untreated Controls Aerobic Anaerobic
Inceotisol
DayO
Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ <LOQ <LOD <LOD CLOQ <LOQ <LOQ <LOD CLOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ CLOQ <LOQ <LOD <LOQ <LOD
LOD *=limit of detection (0.0003 jjmol/kg) LOQ = limit of quantitation (0.0043 umol/kg)
<LOD <LOD CLOD CLOD CLOQ CLOQ CLOQ cLOQ CLOQ CLOD cLOQ CLOQ cLOQ cLOQ
Fortified Recoveries
Aerobic
Anaerobic
0.027 0.025 0.028 0.023 0.023 0.023 0.027 0.023 0.024 0.026 0.030 0.023 0.022 0.021 0.028 0.023 0.019 0.019
124% 116% 130% 106% 107% 107% 125% 107% 112% 119% 140% 105% 104% 100% 130% 109% 89% 88%
0.022 0.022 0.023 0.021 0.022 0.022 0.022 0.023 0.024 0.024 0.024 0.023 0.022 0.023
102% 100% 108% 98% 103% 102% 101% 107% 111% 111% 110% 108% 101% 108%
Friday, O ctober 12, 2007
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W ildlife International, Ltd.
-280-
p. 281 Project No.: 112E-108
Wildiife International, Ltd.
P ro je ct N u m b ers 1 12E -108,109,110
Measured Concentrations of PFDA (pmol/kg) in Untreated and Fortified Soils
Sample
Untreated Controls
Fortified Recoveries
Soil Type________ Interval______ Aerobic Anaerobic_____ Aerobic______ Anaerobic
Ultisol
DayO Week 1 Week 2 Week 4 Month 2 Month 4
Month 6 Month 9 Month 12 Month 15 Month 18 Month 2+
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <L0Q <LOQ <L0D <LOD <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
0.018 0.014 0.017 0.017 0.017 0.017 0.018 0.018 0.021 0.021 0.019 0.019 0.010 0.018 0.016 0.014
93% 74% 89% 86% 88% 89% 94% 93% 109% 109% 96%
100%
52% 95% 84% 70%
0.018 0.018 0.018 0019 0.018 0.018 0.018 0.016 0.020 0.020 0.021 0.021 0.019 0.020
92% 91% 90% 97% 94% 92% 93% 80% 104%
102% 110%
109% 96% 104%
LOD = lim it o f detection (0.0002 pmol/kg) LOQ = lim it o f quantitation (0.0039 pmol/kg)
Friday, October 12,2007
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W ildlife International, Ltd.
-281 -
p. 282 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers 112E-108,109,110
Measured Concentrations of PFDA (junoL/kg) in Untreated and Fortified Soils
Soil Type
Sample Interval
Untreated Controls Aerobic Anaerobic
Mollisol
DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month? Month 12 Month IS Month 18 Month 24
<LOQ <LOQ <LOD <LOD CLOD CLOD cLOQ cLOQ CLOD CLOD cLOD CLOD <LOD cLOD cLOQ cLOQ CLOD CLOD cLOD CLOD cLOQ
CLOQ CLOQ cLOQ CLOD CLOD CLOD cLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD
LGD = limit of detection (0.0002 pmol/kg) LOQ = limit of quantitation (0.0039 unol/kg)
Fortified Recoveries
Aerobic
Anaerobic
0.019 0.019 0.019 0.020 0.019 0.020 0.019 0.016 0.019 0.019 0.023 0.021 0.024 0.022 0.018 0.020 0.015 0.016
100% 100% 98% 104% 97% 101% 98% 81% 99% 97% 117% 109% 121% 112% 92% 102% 76% 80%
0.018 0.019 0.016 0.018 0.018 0.018 0.017 0.018 0.020 0.018 0.022 0.024 0.022 0.021
92% 99% 82% 95% 92% 93% 90% 90% 104% 92% 114% 121% 112% 109%
Friday, O ctober 12, 2007
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W ildlife International, Ltd.
-282-
p. 283 Project No.: 112E-108
Wildlife International, Ltd.
P roject N um bers 1 12E -108,109,110
Measured Concentrations of PFDA (jnnol/kg) in Untreated and Fortified Soils
Soil Type
Sample Interval
Untreated Controls Aerobic Anaerobic
Alfisol
Day 0 Week 1 Week 2 Wed; 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOQ CLOD CLOD CLOD CLOD
LOD = limit of detection (0.0002 ^imol/kg) LOQ = limit of quantitation (0.0039 jimol/kg)
cLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD CLOD CLOQ
Fortified Recoveries
Aerobic
Anaerobic
0.018 0.018 0.018 0.021 0.020 0.017 0.018 0.020 0.023 0.022 0.018 0.021 0.021 0.022 0.021 0.026 0.018 0.018
93% 93% 94% 110% 101% 86% 90% 105% 119% 112% 93% 109% 107% 112% 109% 133% 92% 93%
0.018 0.014 0.017 0.018 0.018 0.019 0.019 0.019 0.022 0.022 0.018 0.019 0.023 0.017
92% 74% 87% 93% 90% 95% 97% 96% 113% 114% 90% 96% 116% 86%
Friday, O ctober 12, 2007
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W ildlife International, Ltd.
-283 -
p. 284 Project No.: 112E-108
Wildlife InternationalLtd.
P ro ject N um bers 112E -108,109,110
Measured Concentrations of PFDA (funol/kg) in Untreated and Fortified Soils
Soil Type
Sample Interval
Untreated Controls Aerobic Anaerobic
Incentisol
Day 6 Weekl Week 2 Week 4 Month. 2 Month 4 Month 6 Month 9 Month 12
Month 15 Month 18 Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ
LOD = limit of detection (0.0002 pmol/kg) LOQ = limit of quantitation (0.0039 pmol/kg)
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
Fortified Recoveries
Aerobic
Anaerobic
0.027 0.024 0.028 0.023 0.022 0.022 0.024 0.022 0.023 0.026 0.027 0.021 0.021 0.018 0.025 0.021 0.018 0.018
139% 124% 146% 116% 113% 116% 121% 115% 120% 133% 141% 108% 107% 92% 128% 109% 95% 92%
0.019 0.019 0.021 0.019 0.021 0.020 0.020 0.021 0.023 0.024 0.021 0.023 0.019 0.021
99% 97% 107% 96% 108% 105% 101% 107% 118% 122% 108% 116% 96% 106%
Friday, O ctober 12, 2007
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W ildlife International, Ltd.
-284-
p. 285 Project No.: 112E-108
Wildlife International, Ltd.
P roject N um bers 112E -108,109,110
Measured Concentrations of PFUA (punol/kg) in Untreated and Fortified Soils
Sample
Untreated Controls
Fortified Recoveries
Soil Type________ Interval______ Aerobic Anaerobic_____ Aerobic______ Anaerobic
UWsoI
Day 0 Week 1 Week 2 Week 4
Mouth. 2 Month. 4 Month6 Month 9 Month 12 Month 15 Month IS Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <L0Q <LOQ
0.015 0.012 0.015 0.014 0.013 0.014 0.015 0.013 0.018 0.018 0.016 0.017 0.007 0.015 0.013 0.011
85% 69% 82% 82% 76% 78% 86% 74% 102% 101% 92% 93% 41% 84% 72% 62%
0.015 0.016 0.015 0.015 0.016 0.015 0.016 0.012 0.017 0.017 0.017 0.017 0.016 0.018
85% 88% 85% 84% 88% 86% 91% 70% 98% 95% 94% 95% 88% 99%
LOD = limit of detection (0.0002 j^mol/kg) LOO = limit of quantitation (0.0036 fimol/kg)
Triday, O ctober 22, 2007
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.W ildlife International, L td
-285 -
p. 286 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers 1 1 2 E -1 0 8 , 109,110
Measured Concentrations of PFUA (punol/kg) in Untreated and Fortified Soils
Soil Type
Sample Interval
Untreated Controls Aerobic Anaerobic
Mollisol
DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12
Month 15 Month 18 Month 24
<LOQ <LOQ <LOD CLOD <LOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD <LOQ <LOQ <LOQ cLOQ CLOD <LOD <LOD <LOD CLOD
cLOQ CLOQ cLOQ cLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD
LOD = limit of detection (0.0002 funol/kg) LOQ = limit of quantitation (0.0036 pmol/kg)
Fortified Recoveries
Aerobic
Anaerobic
0.017 0.017 0.015 0.017 0.016 0.016 0.015 0.012 0.015 0.015 0.018 0.018 0.017 0.015 0.013 0.016 0.011 0.011
95% 96% 87% 93% 90% 92% 87%
69%
84% 84%
101% 101%
95% 87% 74%
88%
61% 63%
0.015 0.015 0.012 0.014 0.011 0.013 0.011 0.014 0.015 0.013 0.017 0.017 0.017 0.014
82% 86% 67% 76% 63% 71% 63% 80% 85% 75% 96% 94% 98% 79%
,Friday O ctober 12, 2007
Page 2 o f4
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W ildlife International, Ltd.
- 286-
p. 287 Project No.: 112E-108
Wildlife International, Ltd.
Project N um bers 1123-108,109,110
Measured Concentrations of PFUA (pmol/kg) in Untreated and Fortified Soils
Sample Soil Type________ Interval
Untreated Controls
Fortified Recoveries
Aerobic Anaerobic_____ Aerobic______ Anaerobic
Alfisol
DayO Week 1 Week 2 Week 4 Month. 2 Month4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24
<LOD <LOD <L0D CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOQ CLOQ CLOD <LOD CLOD
CLOD CLOD CLOD cLOD cLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOQ CLOQ
0.016 0.016 0.016 0.019 0.017 0.014 0.014 0.017 0.018 0.017 0.013 0.016 0.016 0.016 0.015 0.022 0.016 0.016
92% 87% 92% 109% 94% 80% 77% 94% 103% 97% 73% 89% 89% 93% 86% 122% 87% 88%
0.015 0.011 0.015 0.015 0.014 0.014 0.012 0.013 0.016 0.017 0.013 0.013 0.017 0.012
83% 61% 83% 86% 79% 79% 70% 76% 88%
99%
74% 72% 95% 67%
LOD --limit of detection (0.0002 umol/kg) LOQ = limit of quantitation (0.0036 pmol/kg)
Friday, O ctober 12, 2007
Page 3 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-287-
p. 288 Project No.: 112E-108
Wildlife International, ltd .
P ro ject N u m b ers 112E -108,109,110
Measured Concentrations of PFUA (p.mol/kg) in Untreated and Fortified Soils
Soil Type
Sample Interval
Untreated Controls Aerobic Anaerobic
InceDtisol
Day 0
Week 1 Week 2 Week 4 Month 2 Month 4
Month 6
Month 9 Month 12 Month 15 Month 18 Month 24
<LOQ
<LOQ <LOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD <LOD CLOD
cLOQ
<LOQ
cLOQ
<LOQ
CLOQ CLOQ CLOD CLOD CLOD
CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOQ cLO Q
LOD limit of detection (0.0002 pmol/kg) LOQ = limit of quantitation (0.0036 pmol/kg)
Fortified Recoveries
Aerobic
Anaerobic
0.021 0.020 0.022 0.018 0.016 0.018 0.017 0.016 0.018 0.021 0.022 0.016 0.014 0.015 0.018 0.015 0.013 0.012
120% 112% 125% 101% 92% 103% 98% 89% 104% 117% 122% 88% 82% 83% 99% 82% 74% 69%
0.014 0.013 0.014 0.013 0.013 0.015 0.014 0.015 0.015 0.018 0.012 0.013 0.012 0.015
79% 74% 79% 74% 74% 87% 80% 85% 87% 103% 69% 73% 70% 85%
>Friday O ctober 12, 2007
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Company Sanitized. Does not contain TSCA CBi.
W ildlife International, Ltd.
-288 -
p. 289 Project No.: 112E-108
Wildlife InternationalLtd.
Project Numbers 112B-108,109,110
Measured Concentrations of 8-2 FTOH (jtmol/kg) in Untreated and Fortified Soils
Sample
Untreated Controls
Fortified Recoveries
Soil Type________ Interval______ Aerobic Anaerobic_____ Aerobic______ Anaerobic
Uhisol
Dayo Week 1 Week 2 Week 4 Montk 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24
<LOD <LOD <LOD <LOD <LOD CLOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD
CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD
0.622 0.609 0.525 0.531 0.452 0.448 0.356 0.317 0.220 CLOQ cLOQ CLOQ CLOQ CLOQ CLOQ CLOQ cLOQ CLOQ
115% 113% 97% 99% 84% 83% 66% 59% 41% 39% 18% 21% 16%
9% 9% 0% 0% 0%
0.627 0.621 0.475 0.555
116% 115% 88% 103%
0.536 0.390 0.385 0.334 0.356 0.253 CLOQ CLOQ cLOQ
99% 72% 71% 62% 66% 47% 35% 22% 27%
LOD = limit o f detection (0.048 pmol/kg) LOQ = limit ofquantitation (0.216 pmol/kg)
M onday, O ctober 25, 2007
Page 1 o f4
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W ildlife International, Ltd.
-289 -
p. 290 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers 112E-108,109,110
Measured Concentrations of 8-2 FTOH (pmol/kg) in Untreated and Fortified Soils
Sample
Untreated Controls
Fortified Recoveries
Soil Type________ Interval_______Aerobic Anaerobic_____ Aerobic______ Anaerobic
MoDisol
Da? 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month IS Month 24
<LOD <LOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD <LOD CLOD CLOD CLOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD
CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD
0.645
0.562 0.459 0.469 0.419 0.241 0.256 cLOQ cLOQ cLOQ CLOQ cLOQ cLOQ CLOQ cLOQ cLOQ CLOQ
120%
104% 85% 87% 78% 45% 48%
21%
25% 7% 13% 3% 4% 0% 3% 0% 0%
0.574 0.602 0.526 0.589 0.554 0.524 0.412 0.413 CLOQ 0.349 cLOQ cLOQ cLOQ CLOQ
106% 112% 98% 109% 103% 97% 77% 77% 40% 65% 28% 18% 14%
6%
LOD = limit of detection (0.048 pmol/kg)
L O Q = lim it o f quantitation (0.216 pm ol/kg)
M onday, O ctober IS , 2007
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W ildlife International, Ltd.
-290-
p. 291 Project No.: 112E-108
Wildlife International, Ltd.
ProjectNumbersll2E-108,109,110
Measured Concentrations of 8-2 FTOH (junol/kg) in Untreated and Fortified Soils
Sam ple
Untreated Controls
F ortified R ecoveries
S o il Type__________ In terval________ A erobic A naerobic______ A erobic_______ A naerobic
Alfisol
DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOD <LOD CLOD <LOD CLOD <LOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD cLOD CLOD cLOD
CLOD CLOD CLOD CLOD cLOD CLOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD
0.662 0.527
0.453 0.309 0.336 0.307
0.252 0.241 cLOQ <LOQ cLOQ CLOQ cLOQ CLOQ cLOQ CLOQ cLOQ CLOQ
123% 98% 84% 57% 62% 57% 47% 45% 30% 32% 22% 21% 5% 12% 4% 7% 0% 0%
0.574 0.548 0.462 0.437 0.441 0.347 0.274 0.308 CLOQ cLOQ cLOQ CLOQ cLOQ CLOQ
107% 102% 86% 81% 82% 64% 51% 57% 33% 25% 20% 15%
4% 7%
LOD = limit of detection (0.048 pmol/kg) LOQ = limit ofquantitation (0.216 pmol/kg)
M onday, October IS , 2007
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W ildlife International, Ltd.
-291 -
p. 292 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers 1123-108,109,110
Measured Concentrations of 8-2 FTOH (junol/kg) in Untreated and Fortified Soils
Sample
Untreated Controls
Fortified Recoveries
Soil Type________ Interval______ Aerobic Anaerobic_____ Aerobic______ Anaerobic
Incentisol
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4
Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
<LOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD <LOD CLOD cLOD <LOD cLOD cLOD cLOD CLOD CLOD CLOD CLOD cLOD
CLOD
LOD = limit of detection (0.048 pmol/kg) LOQ = limit of quantitation (0.216 pmol/kg)
CLOD CLOD CLOD CLOD CLOD CLOD cLOD <LOD CLOD CLOD CLOD CLOD cLOD CLOD
0.613 0.565 0.575 0.613 0.529 0.505 0.470 0.612 0.434 0.430 0.338 0.401 cLOQ cLOQ <LOQ 0.248 cLOQ cLOQ
114% 105% 107% 114% 98% 94% 87% 114% 81% 80% 63% 74% 34% 34% 22% 46%
0% 0%
0.619 0.613 0.555 0.560 0.511 0.507 0.495 0.471 0.289 0.356 0.228 0.228 cLOQ CLOQ
115% 114% 103% 104% 95% 94% 92% 87% 54%
66%
42% 42% 17% 15%
M onday, O ctober 15, 2007
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W ildlife International, Ltd.
-292 -
p. 293 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers m E - ,1 0 8 1 0 9 ,1 1 0
Measured Concentrations of 8-2 FTOH (jimoI/L) in Headspace Gases from Untreated and Fortified Soils
Sam ple
Untreated C ontrols
F ortified S oils
S o il Type__________ In terva l______ A erobic A naerobic_______A ero b ic A naerobic
Ultisol
DayO Week 1 Week 2 Week4 Month.2 Month4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOQ = limit of quantitation (0.027 jimol/L ofheadspace)
M onday, O ctober IS , 2007
P agel o f4
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W ildlife International, Ltd.
-293 -
p. 294 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers 112E-108,109,110
Measured Concentrations of 8-2 FTOH (pmol/L) in Headspace Gases from Untreated and Fortified Soils
S oil Type
Sam ple In terval
U ntreated C ontrols A erobic A naerobic
Mollisol
DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month IS Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOQ = limit of quantitation (0.027 pmoVL ofheadspace)
F ortified S oils A erobic A naerobic
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
M onday, O ctober 15, 2007
Page2 o f4
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W ildlife International, Ltd.
-294-
p. 295 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers 11 2 E-108,109,110
Measured Concentrations of 8-2 FTOH (junol/L) in Headspace Gases from Untreated and Fortified Soils
S oil Type
Sam ple In terval
U ntreated C ontrols A erobic A naerobic
Alfisol
Day 0 Week 1 . Week2 Week4 Month2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ , <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
`
LOQ = limit of quantitation (0.027 pmol/L ofheadspace)
F ortified S oils A erobic A naerobic
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ . <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
-
M onday, October IS , 2007
Page 3 o f4
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
-295-
p. 296 Project No.: 112E-108
Wildlife International, Ltd.
Project Numbers 112E-108,109,110
Measured Concentrations of 8-2 FTOH (pmol/L) in Headspace Gases from Untreated and Fortified Soils
S oil Type
Sam ple In terval
U ntreated C ontrols A erobic A naerobic
Incentisol
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
LOQ = limit of quantitation (0.027 pmol/L of headspace)
F ortified S o ils A erobic A naerobic
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ
M onday, O ctober 15, 2007
Pap: 4 o f4
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Company Sanitized. Does not contain TSCA CB1.
.W ildlife International, Ltd
Project No.: 112E-108
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Appendix III Example Calculations Standard Curves by Linear Regression
Standard curves were prepared by plotting the ratio of analyte concentration (pg/L ng/mL) o f 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA, PFUA, 7-2 sFTOH and 7-3 Acid to the internal standard concentration (0.75 ng/mL 2-13C PFOA for 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA, PFUA and 7-3 Acid and 100 ng/mL HDFD
for 8-2 FTOH and 7-2 sFTOH) in solutions with known concentrations on the abscissa and the respective peak area responses on the ordinate. Weighted regression (1/x) was applied to analysis of the data to determine the equation with respect to the abscissa as shown below:
A = [ m x ( C / Q ) + b] x A;
where A =
Ai =
m C= Ci = b=
analyte area internal standard area slope
analyte concentration (ng/mL) internal standard concentration (ng/mL) intercept
Concentrations o f analytes in the final solutions of processed samples, just prior to injection, were calculated using a rearrangement o f the above equation:
c _ C, x (AJAj) - b m
Residue Concentrations of 8-2 FTOH. 8-2 FTA. 8-2 FTUA. PFOA. PFNA. PFDA. PFUA, 7-2 sFTOH and 7-3 Acid in Soil Extracts
Residue concentrations (pg/kg) of 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA, PFUA, 7-2 sFTOH and 7-3 Acid in soil extracts were determined using the following equation:
Sample Concentration in pg/kg =
(V ^
where
C = Concentration (ng/mL) as determined above Ve = Extraction volume (125 mL) V;i = First initial volume (mL)
Vi2 = Second initial volume (if required) Vf, = First final volume (mL) Vf2 = Second final volume (mL)
W = Weight of sample (25 g dry weight).
Company Sanitized. Does not contain TSCA CBI.
W ildlife International, Ltd.
p. 298 project n o : 112E-108
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Residue concentrations (pmol/kg) of 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA, PFUA, 7-2 sFTOH and 7-3 Acid in soil extracts were determined using the following equation:
Sample Concentration in pmol/kg = C x molecular weight (pmol/pg)
where C = Sample concentration (pg/kg) as determined above
C. Procedural Recoveries in Fortified Controls
Procedural recoveries were calculated for fortified control samples prepared in each test condition at known concentrations in 8-2 FTOH (250 pg/kg), 8-2 FTA (10.00 pg/kg), 8-2 FTUA (10.00 pg/kg), PFOA (10.00 pg/kg), PFNA (10.00 pg/kg), PFDA (10.00 pg/kg) and PFUA (10.00 pg/kg). Percent recoveries of the each analyte were determined to provide confirmation that the method was performing adequately. Percent recoveries were determined by dividing the concentration of the analyte recovered in the fortified sample by the nominal concentration added as shown below:
Percent Recovery = gg/kgFoun^ x i 00% pg/kg Added
be* oO
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.W ildlife International, Ltd
Sampling Interval
Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24
-2 9 8 -
Appendix IV Duration of Incubation
Actual Days of Incubation
Alfisol 0 7 14 28 63
119 182 273 365 462 546 728
Inceptisol 0 7 14 28 63
119 182 . 273 365 462 546 728
Ultisol 0 7 14 28 56
119 182 273 364 462 553 728
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Project No.: 112E-108
Mollisol 0 7 14 28 56
119 182 273 364 462 553 728
C o m p a n y Sanitized
