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AR16-0375 estsvestnee ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity: Perfluorooctanesulfonyl fluoride; may also be referred to as POSF, or FX-8. (1-Octanesulfonyl fluoride, 1,1,2,2,3.34,4,5,5,6,6,7,7,8,8,8-heptadecafluoro-, CAS #307- 35-7). wewo Remarks: The test substance is a clear liquid of uncharacterized purity. Itis described as FX-8 (ccB42-2) washed sample. METHOD Method: Protocol Reference EAR/WAS 5/9/84 Test type: Not noted. GLP: No Year completed: 1984 Analytical monitoring: Dissolved `oxygen concentrations. Stock and test solution preparation: Test substance (0.309 g) weighed directly into BOD bottle, 50 mL deionized water was added, and the bottle mixed on a magnetic mixer for ~ 30-minutes. Bottle was cappedduring Inoculum source: Activated sludge mixed liquor from the Metro `wastewater treatment plant -- St. Paul/Minneapolis, MN. Inoculum holding: None - used the day it was obtained. Element basis: Respiration inhibition as determined by oxygen consumption. ews Method remarks: After mixing in the BOD bottle, it was noted that the sample settled to the bottom, forming small droplets or beads. Some of the sample appeared to emulsify. RESULTS Nominal concentrations: 1000 mg/L test material solution. Element value: 6.75-min ICso = > 1000 mg/L covwsows Control response: Adequate -- 6.5% difference in respiration rateof the two controls. CONCLUSIONS The test material exhibited no inhibitory effect at 1000 mg/L ovear 6.75minute exposure. `Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 0066177 eiDAfTAQQoUaALrITyY 0 Reliability: approved. Kimisch This study ranking 3. The lacks sufficient method used was not agency documentation, characterization of the test substance concentrations. purity, and analytical measurement of test SReEFeEmReENNeCeEsS 00000000 TLahbiosrsattuordyy,wSats. Pcaounld,ucMtNe,dLbaybtRheeq3uMesCtonmupmabneyr, BE1n2v2i9r.onmental eOmTHeERr Last changed: 5/18/00 006618 3H Environmental Laboratory ActivRaetuetde_STluodxgelcltyHoxeodf aLiSquubosrta(nScLeUDGtEo-TOX) Protocol Reference: ERR/WRS 5/08/84, Lob Request No. B1229 8 7 s s =s =g COR3 g> g2 s a : \ N Legend |O DEIONIZED WATER CONTROLS - AVERAGE A pi229-2,rs, Coes2-2, wasHED, (1000 Wan) ICS0 = >1000 MB/L N\ \\ . A \ 1 o v1 5 1 & 7 / TIME - MINUTES od Wa st STOX.DAT 006619 wa rast GS Ju" 05 - Acute Toxicity of 8 3M rEnavitroenmRenetafloLrabmosractioaryted Sits me Liquor E ress SE ovens S)i= 50 wmarfpdSseelil * MTheetroacWtaisvtaetweadtesrludPgleantmioxned 1iquoJriew,asZYcollec. ted from the St. Paul/Minneapolis Sample description: 2 (Bra2a-2 X-8) (C8Ya-2, wasied I I -- aT err D Seene.atomenomsiTenfgxeLntwra,ters OTE TT pimps1 were Te edo m LdID|2bs2e 1D |[][T epaTrlsree eeeo sws]e|[fTlol asaeseoeT |]T o n|TTe TJLT[ezEool oeeOweT eT[T TbIa rlTr TTTo1[moo05oae 07nrr0]]|]|] Po fp rn TTT LT COMMENTS: CAT oo, 0.53;0 gf dongle am.r phoe nt mi oo a sone foe 230 pin Kn dss L or sM eppoantd|e2!wiA]ptdeTegd pof obs eli), Mom oeising Ts TL frre ese eptember dom ooff fHios emppompl ef fran L o Svooidnidt 3-27-84 ENVIRONMENTAL LABORATORY STATUS REPORT ALL RESULTS TENTATIVE 1EQUESTER NAME: JEPT: 0535 ANW/EAR J'ARTOEJERCTECENIO:VED9:970021-22610-084 JESC: FX-8 (CC 842-2) SAMPLE UMBER NUMBER ONE. TWO WILL BE LAB REQUEST NO. B1229 GENERATED FROM TWO EQUAL CONTRACT LAB: CONTRACT LAB 3M E-L HOURS: COST: 62 0 DEASTTE CCMOPMLPTLEDTAETDE:: 5-01-84 VOLUME WASHES OF SAMPLE SAMPLE DATE 1 2 CODE DESCRIPTION FX-8 WASHED ~~ SAMPLE *# == UCPODNATTREADCTDALTAAB TEST SMPL-PREP. 96-HR-LC50 -B0D-05 -BOD-10 -B0D-20 SOLUBILITY BIOSORP JEG-SPEC SLUDGE-TOX RESULT COMPLETE #SEE REPORT # 006621 4-11-94 ENVIRONMENATLALLRELSAUBLOTRSATOTREYNTASTTIAVTEUS REPORT LAB REQUEST NO. B1229 DREEPQTU:EST0E5R35 NAME: ANW/EAR PROJECT NO: 9970012500 CCOONNTTRRAACCTT Las: LAB COST: 0 DDEASTCE: REFCXE-3IVE(DC:C 9422--221)-84 E3SMT E-CLMPLHTOURDSA:TE: 47 5-01-84 DATE COMPLETED: NSUAMMPBLEER NUMBER ONE. TWO WILL BE GENERATED FROM TWO EQUAL VOLUME WASHES OF SAMPLE SAMPLE DATE 1 2 cope DESCRIPTION FX=8 WASHED ~ SAMPLE #* == UCOPNDTARTAECDT DLAATBA TEST SWPL-PREP C9-5B=OHDR--0L5C50 CC--BB0ODD--2100 BSIOOLSUOBRIPLITY SLUDGE-TOX RESULT COMPLETE SSEEEE RREEPPOORRTT #SEE REPORT 006622