Document qdJQKyOa0NDzEKXQNKQZ8gXEK

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G..DAPHNIA) TEST SUBSTANCE Identity: Perfluorooctanoic add; may also be referred to as PFOA, FC26, or FX-1001. (Octanoic add, pentadecafluoro-, CAS # 33567-1) Remarks: The 3M production lot number was 269. The test substance is a white powder. The test sample is FC-26 referred to by the test laboratory as N2803-3. The purity of the sample was not sufficiently characterized, although current information indicates it is a mixture of 96.5 -1 0 0 % test substance and 0 - 3.5% Ce, C7, and C9perfluoro homologue compounds. _ Remarks field: The sample preparation directions given to the laboratory were to dissolve the test material in a 50:50 watenisopropanol solution. In the protocol amendment, it was stated that the sample was combined with isopropanol in a 50:50 ratio prior to use. METHOD Method: U.S. EPA-TSCA Guideline 797.1300 Test type: Static acute GLP: Yes Year study conducted: 1995 Year study completed: 1996. Species: Daphnia magna Analytical monitoring: Nominal concentrations. DO, conductivity, pH, and temperature were monitored daily. Exposure period: 48-hours Statistical methods: LC50 and EC50 values calculated, when possible, by probit analysis, moving average method or binomial probability with non linear interpolation using the computer software of C.E. Stephan. Test daphnid source: Obtained from cultures maintained by T.R. Wilbury Laboratories Inc, Marblehead, MA from an original culture from Aquatic Research Organisms, Hampton, NH. Test daphnid age at study initiation: < 24-hours Test conditions Dilution water: Filtered, deionized water adjusted to a hardness of 160 mg/L as CaCOVL Dilution water chemistry: Representative sample given in Table 1 of the final report. Temperature: 1 9 .4 -2 0 .2 C C03993 Lighting: Cool-white fluorescent bulbs with an intensity of 58 ft-c. Photoperiod of 16-hours light, 8-hours dark with a 15 minute transition period. Stock and test solutions preparation: A 1,000 mg/L primary stock solution (50:50 mixture of test substance and isopropanol) was prepared in dilution water. After mixing, the primary stock was proportionally diluted with dilution water to prepare the test concentrations. No insoluble material was noted during the test. Exposure vessels: 300 mL glass beakers containing 250 mL of test solution. The approximate depth of test solution was 9 cm. Number of replicates: two Number of daphnids per replicate: ten Number of concentrations: five plus a negative control Water chemistry during the study: Dissolved-oxygen range (0 - 48 hours): 8.7 - 8.8 mg/L (control exposure) 8.7 - 8.8 mg/L (1,000 mg/L exposure cone.) Conductivity range (0 - 48 hours) 590 - 610 pmhos/cm (control exposure) 570 - 590 pmhos/cm (1,000 mg/L exposure cone.) pH range ( 0 - 4 8 hours) 7.9 - 8.4 (control exposure) 7.5 - 8.0 (1,000 mg/L exposure cone.) Test temperature range (0 - 48 hours) 19.5 - 20.2C (control exposure) 19.4 - 20.2C (1,000 mg/L exposure cone.) Element basis: mortality and immobilization RESULTS Nominal concentrations: Blank control, 130, 200, 360, 600,1,000 mg/L ( Tested as a 50:50 mixture of test substance and isopropanol). The concentration of test substance in solution was 65, 110,180, 300 and 500 mg/L Element value (as-tested): 24-hour ECso = 840 (740 - 970) mg/L 24-hour LCso = 1,000 (600 - >1,000) mg/L 48-hour ECso = 720 (660 - 780) mg/L 48-hour LCso = 800 (700 - 920) mg/L 48-hour NOEC = 360 mg/L Element value (based on concentration of test substance in solution): 24-hour ECso = 420 (370 - 490) mg/L C-03994 24-hour LCso = 500 (300 - >500) mg/L 48-hour ECso = 360 (300 - 500) mg/L 48-hour LCso = 400 (350 - 460) mg/L 48-hour NOEC = 180 mg/L All element values based on nominal concentrations. A toxicity test conducted previously with isopropanol at 390 mg/L showed no mortality or sublethal effects. Statistical Evaluation: The LCso and ECso values and 95% confidence intervals were calculated when possible by probit analysis, the moving average method or binomial probability with non-linear interpolation using the computer software of C.E. Stephan. Biological observations: Daphnids in the control, the 130, 200, and 360 mg/L treatments appeared healthy and normal throughout the test with no mortality, immobility or overt clinical signs of toxicity. Cumulative percent mortality: Nominal A s-Tested Cone., m g/L 24-hours 48-hours Control 130 200 360 600 1,000 0 0 0 0 10 50 0 0 0 0 15 80 Control response: satisfactory CONCLUSIONS The as-tested test substance 48-hour LCso for Daphnia magna was determined to be 800 mg/L with a 95% confidence interval of 700 - 920 mg/L. The as-tested test substance 48-hour ECso for Daphnia magna was determined to be 720 mg/L with a 95% confidence interval of 660 - 780 mg/L. The as-tested test substance 48-hour no observed effect concentration (NOEC) was 360 mg/L. The 48-hour LCso for Daphnia magna based on the concentration of test substance in solution was determined to be 400 mg/L with a 95% confidence interval of 350 - 460 mg/L. The 48-hour ECso for Daphnia magna based on the concentration of test substance in solution was determined to be 360 mg/L with a 95% C03995 confidence interval of 300 - 500 mg/L. The 48-hour no observed effect concentration (NOEC) for the test substance in solution was 180 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 3. The study lacks analytical measurement of test substance concentrations in the test solutions and sample purity is not sufficiently characterized. Additionally, there appears to be a discrepancy between the sample preparation directions given to the laboratory and the procedure conducted by the laboratory to prepare the test solutions. -- REFERENCES This study was conducted at T.R. Wilbury Laboratories, Inc., Marblehead, MA, at the request of the 3M Company. OTHER Last changed: 5/25/00 003996 r -'i : Study Title Acuta Toxicity of N2803-3 to the Daphnid, Daphnla augna Data Rsquireawntt Ouidalina Number U.S. EPA-TSCA, ouidalina 797.1300 Authors Timothy J. Hard Joanna P. Magazu Robert L. Boeri Study Coapletod Harch 7, 1996 Sponsor :f W : Mi mW-1 - %', OOOD 1AHOXATOKY PSACT1C* CONFLXAMC* STATSMSWX * \, This study wss conducted according to IPX Good Laboratory Practice Regulations (40 CFR 792), with the following exception. The stability of, the test substance was assumed but not verified at t .r . wilbury Laboratories. Deviations from the study protocol and protocol amendments are presented in section XII of this report. Magatu, Study Director ' COPY A W T.R. Kilbury Laboratorlea, Inc. atudy number 892-TH, M u t e Toxicity of N2803-3 to the Daphnid, Daphnla aagna, waa audited by the Quality Assurance Unit for compliance with the protocol, atandard operating procedures, and applicable Good Laboratory Practicea (U.S. EPA, 1993). Quality aaaurance audits were performed and the findings reported to T.R. wilbury Laboratories management and the study director on the following datesi Aspect Audited Protocol: In-life: Draft Report: Pinal Report: Audit Date B/ 4/95 9/ 1/95 9/ 6/95 1/ 2/96 3/7/96 Reported to Study Director 8/4/95 <9/ 1/95 9/ 6/95 1/ 3/96 ,,3/,7/96 Reported , to Management 8/ 4/95 3/ 7/96 3/7/96 3/ 7/96 3/ 7/96 4 -k `4^ C0399 BEST COP* III. T A B U OF COMTXMTa SECTIONI I. Good laboratory Practice Compilane Statamant II. Quality Aaauranca statement III. Table of Contenta XV. ' Index of Tables andrigures V. summary VI. Cenerai Information VII. Introduction VIII. Methods and Materials IX. Results X. References X I . ,1' Signature Page l 'XIX. Protocol Amendments and Deviations -'.v V Appendix A. Hater Quality Data from Toxicity Test PACE 2 3 'V <9V,: 'v*' p - t * . : 1'-: ' ></' M *5 ,r 6 7 a 8 . 13 18 * < , 19 ;iv : 20 * "T'i' 21 '.u <,\<f-i f f -li Y * , fc04000 , i . > "S ' v- >** '* . .*.-</ ' : :r* ^ V x S-k - ^.fT .. *,<, JL ;.,V,srlf-rf >* ' W ' ^ 4- J-- vi `'Ji*;' ^ Y\,- 'Y Y v . :V.v.- . vx>; `u s| | > : .'C^M: << f l f e v V hi/-.Aj'S-r,*''--*' 004001 BEST COPY AYAH AILE V. SUMMAAX Tha acuta toxicity of N2803-3 to the daphnid, Daphnla magma, is daacribad in this final raport. Tha taat was conductad for 3K coaipany for 48 hours from August 30 to September 1, 1995, at T.R. Wilbury Laboratories, Inc. in Karblahaad, Massachusetts. It was conductad by Jaanna Kagazu, Peter Kowalski, Jacqueline Nevius, Keven Stevens, Peter Hevius, Robert Boari, and Timothy Ward according to tha protocol developed for T.R. Wilbury study Humber 892-TH. H2803-3 was supplied by tha sponsor. The test was performed under static conditions with five concentrations of test substance and a dilution water control at a temperature of 19.4-20.2*C. The dilution water was filtered daionized water collected-et Marblehead, Massachusetts and adjusted to a hardness of 160 mg/L as CaCO}. Aeration was not required to maintain dissolved oxygen concentrations above an acceptable level. The sample of B2803-3 supplied by the Bponaor was combined with isopropanol in a 50:50 ratio prior to use (a toxicity test was conducted with 390 mg/L isopropanol and no mortality or aublethal effects were observed). Nominal concentrations of N2803-3 as-received were 0 (control), 65, 110, 180, 300, and 500 mg/L. Daphnids used in the test were less than 24 hours old at the start of the test. They were produced at T.R. wilbury Laboratories and acclimated under test conditions for their entire life (cultures that produced test specimens were maintained at test conditions for more than 7 days). After 48 hours of exposure the control daphnids had an average wet weight (blotted) of 0.53 mg. All daphnids were in good condition at the beginning of the study. > / Exposure of daphnids to N2803-3 resulted i n ' 2 4 hour median lethal concentration (LC50) of 500 mg/L N2803-3, a 48 hour LC50 of 400 mg/L N2803-3, a 24 hour median effective concentration (EC50) of 420 mg/L, and a 48 hour EC50 of 360 mg/L N2803-3, based the concentration of N2803-3 in the test substance. The 48 hour no observed effect concentration (NOEC) is 180 mg/L, based on the concentration of N2803-3 in the test substance. The 24 hour LC50 ia 1,000 mg/L, the 48 hour LC50 is 800 mg/L, the 24 hour BESTCOPY AVA1IABLE BEST COPYAYAHABIE V* VII. ximtODUcrxoN This study was sponsored by 3m Company, st Paul, Minnesota. The objective of tha study was to determine tha 24 sod 48 hour madian lathal concentrations (LCSOs) and madian affactive concentrations (EC50s> of tha test substance to tha daphnid, Dmphnit magna, a freshwater invertebrate, under static conditions. Tha test protocol was based on U.s. EPA methods (U.S. E7A, 1993). Tha report contains sections that describe the methods and materials employed in the study, and results of the investigation. The report also contains an appendix that presents water quality data collected during the test. VIII. METHODS AMD MATERIALS TEST SUBSTANCE). The sample of N2803-3 (T.R. Wilbury Laboratories Sample Number 521) was delivered to T.R. wilbury Laboratories on August 14, 1995 in a cardboard box at ambient temperature. It was contained in an approximately 50 ml glass jar. The label attached to the bottle included the following information: "N2803-3". N2803-3 (a white solid) was supplied by 3M Company, St Paul, Minnesota. Prior to use the N2803-3 was stored in the dark at room temperature. The sample of N2803-3 supplied by the sponsor was combined with isopropanol in a 50:50 ratio prior to use. This 50:50 mixture was then considered to be 100 test substance during the performance of the toxicity test but all results are reported on an active ingredient basis (active ingredient is the test substance as-received minus the 'isopropanol). The test substance was assumed to have a purity of 100 active ingredient and to be stable under storage and testing conditions. Unused N2B03-3 will be returned to the sponsor. .v,-' DILUTION WATER) . , ,r Water used for acclimation of test organisms and for all toxicity testing was deionized water collected at T.R. Wilbury Laboratories in Marblehead, Massachusetts. Water was adjusted to a hardness of 160- 180 B9/'1* CacOj and stored in 500-gallon polyethylene tanks where it was aerated and continuously passed through a particle filter/' ultraviolet sm tW erv*i4 l^ 4izaeea ra, aa mnd aa cm t^ i1 vaa tA.ea dJ ca aa rm b^.oa nm . .MRa ea s.*uTlAta s..,^o^fv*-c.h_e__mJia caal*.analysif s .ofm a* a ^representative sample of water are presented'in Table4;1 ' ' '_ V.'.vi .- ORGAJtlSMt ii ****** f ' __ ___ ,. Juvenile" daphnida,; D aphnia ` }u tgna?employed S*m ss!.than'i24;hours.oldat:thestartJyof'the'4.tei S s ^',c'""^^ u "\Jlw;Wree^r ed^eni dtiefntdi f,i.e:ds.': 'u's-i-h^g--^'w^--J"i p" p-r o p r t f f l | i M ^ ^ ^ ^ i T e s t l , {specimens were"produced by'adult >daphnids',that^mr^ma'inained' underXtst'M ^conditions' atAthe"T.R.;'Wilbury facility for "more r t h a n g ^ d a y s . " f ^ ^ ^ ^ ^ ^ i&iy>! Vv Vii"'>.vV -r> T.R; Wilbury{study Number 892-TH {Page 8, of' IS .. ...... -|5I I 'i ' M ^ * vi - ' >o '5 t H* -uf * *7 <iv i*"' f * *x r ' > ''r KST COPYAVAI ABLE si: a ,1 ;.v ` * z, ' Tabic 1. Chemical characterization of a representative aample of dilution water uaed for the toxicity teat with daphnida, Daphnla magna, and N2803-3. Parameter1 unit of Measurement Detection . Limit Meaaured Value Metals Aluminum Arsenic Boron Cadmium Chromium Cobalt" copper iron Lead Mercury Nickel silver zinc mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L . 0.1 - 0.01 0.5 0.0002 0.01 0.03 0.005 0.03 . 0.005 .,."t .0.0003 0.03 0.02 0.02 Nitrate Chloride Fluoride Total Organic Carbon Total Phosphorus mg/L as N mg/L mg/L mg/L mg/L 0.05 1 0.1 1 0.03 Organochlorine Pesticides Toxaphene pg/L pg/L ' 0.5 2,, organophospborus Pesticides .Dimethoate ' ( TEPP. .. Y- \ ^ %Monocrotopho* fe`A I"' pg/L pg/L pg/L pg/L " pg/L`- r?$r, |MoteeGj isvja Paranetera dilution `analyzed 'atig^Mf -- - not.datectediat myiM 4f: ft 0.5 2.0_ ND3 NO HD ND HD ND ND 0.03 KD ND ND ND ND ND ND ND ND ND ND ND ND .v ND / 'is fl} t*T.K, ;Wilbury Study Numbar 892-TH **> iS*r vi & '& .-.-f,.. f ' ~>'Vr JSrPW'l P i p a g e .9,,of 25 t-fr,-3^1'$.> -CJ'> ,Jt ' . x ,, ;tk.- 400i BEST COPV AY The original culture vaa obtained on June 1, 1994 iron Aquatic Research organiana, Hampton, New Hampahira. Kaaauramanta made during tha ' 7 daya before the atart of the teat with N2803-3 indicated thati:thajf&%4-'V culture temperature ranged from 19.5 to 20.5*c and the diaaolved oxygen'^i^!';?;;. concentration waa alwaya at leaat 9.1 mg/L and meaauremante made during-`U * 'i'"' the 7 daya before the atart of the teat with iaopropanol indicated that.Vj&<,.. the culture temperature ranged from 20.1 to 20.9*C and the diaaolved oxygen concentration waa alwaya at leaat 8.3 mg/L. The culturea each - produced young prior to day 12 and a eubaample of adults produced at leaat 3 young/daphnid/day for 7 daya before the start of the teats. During acclimation daphnids wars not treated for disease, they were ' free of apparent sickness, injuries, and abnormalities at the beginning of the teat and there was no mortality during the 48 hours preceding the start of the teat. The culture waa supplied with a yeast/trout chow'mix (Loti DC02+- and the freshwater alga, Selanaatrum capclcornuCum, daily during acclimation. Daphnids were not fed during the test. Control daphnids, blotted and weighed at the conclusion of the toxicity test with r s- N2803-3, averaged 0.53 mg and the loading rate during the definitive' toxicity test was approximately 10.4 mg/L and 40 daphnids/L. . i j, TOXICITX TESTING l A static screening test was conducted from August 23 to 25, 199S with five concentrations of test substance and a dilution water control. Nominal concentrations of N2B03-3 were 0.050, 0.50, 5.0, 50, and 500 mg/L. After 48 hours of exposure there was at least 95% survival in the control > and at 0.050, 0.50, 5.0, and 50 mg/L and 15% survival at 500 mg/L ' (surviving daphnids exposed to 500 mg/L were immobilized). ' ^ The definitive toxicity test was performed from August 30 to September 1, 1995, according to T.R. Wilbury Protocol 892-TH (Acute' Toxicity of N2803-3 To The Daphnid, Daphnla magna). The protocol, which : was signed by the study director on August 16, 1995, is based \ on procedures of the U.S. Environmental Protection Agency (1993). A Bummaryfe. of the test system is presented in Table 2. S' - i The test was conducted at a target temperature of 20 t l"c with ive| concentrations of test substance and a dilution water control. * Nominal| concentrations of the test substancewere: 0 mg/L (control), ;1 3 0 ^ 2 0 0 ^ 360, 600, and 1,000 mg/L (concentrations of H2803-3 were 65, 110,`i3qo|and b y - combining ;2.0j:g .of test .fsubstance^(50:50 ;mixture;-of. N2803- isopropanol) and wr w adjusting,the'^fInal's tock'soiutionlMiume" to 2, OOg'^'MilS^BtocVSi vae'l'^xi% ahd'ithehi>ppropSat^aiTOunts|bfi},thiB^stiIt o^dilutioi0," "H' '' aolvent'. BESTCOP 4`, Z Species: ' -'V Age/siza of Test Organisms: Tast Duration: Test Vessels: Test Media Volume: Depth of Test -Media: Number of Replicate Test Vessels: Organisms per Replicate: Temperature Range: Minimum Dissolved Oxygen: Photoperiod: Light Intensity: -s Water Quality Measurements: : Dissolved oxygen, pH, temperature, and conductivity daily in each test . vessel. Hardness, total organic carbon, and alkalinity once in the - control. Residual chlorine daily. Dilution water analysis is summarized ; in Table 1. j' End Points 24 and 48 hour LC50 and EC50 (based on A ; :* S t sfc.X S t e m .4,;- S .s m k f 04007 BEST COPYAYAIlilBLE Twenty daphnids war* indiscriminately and equally distributed among two raplicataa of aach treatment. The teat waa performed in 300 ml glaaa beakers that contained 250 ml of teat aolution (water depth waa approximately 9 c m ) . Teat veaaels were randomly arranged in an incubator during the 48 hour teat (a random numbers table waa uaed to aelect the location of each vessel) and they were loosely covered. A 16 hour light and 8 hour dark photoperiod with a 15 minute transition period waa automatically maintained with cool-white fluorescent lights that provided a light intensity of 58 footcandlea. Aeration was not required to maintain dissolved oxygen concentrations above acceptable levels and daphnids were not fel during the test. The number of surviving organisms and the occurrence of sublethal effects (immobilization, loss of equilibrium, loss of reflex, excitability, discoloration, or change in behavior) ware determined visually and recorded initially and after 24 and 48 hours. Dead test organisms were removed when first observed. Dissolved oxygen (YSI Model 57 meter; instrument number 1), pH (Beckman model pHl 12 meter; instrument number 124), conductivity (Cole Parmer meter, instrument number 94), and temperature (Beckman model pKI 12 meter; instrument number 124, probe pBT4) were measured and recorded daily in each test chamber that contained live animals. The temperature in a beaker of water incubated among tae test vessels was recorded continuously during the test. A static test was conducted from October 4 to 6, 1995 with 390 mg/L isopropanol (0.125 ml isopropanol brought up to 250 ml with dilution water) and a dilution water control. The test waa established with 2 replicates of each concentration and 10 daphnids per replicate and it was conducted in a manner similar to the teat with N2803-3. Test vessels were randomly arranged in a water bath and the light intensity was 13 to 25 footcandles. STATISTICAL METHODS; Results of the toxicity test were interpreted by standard statistical techniques. Computer methods (Stephan, 1983) were used to calculate LC50s and EC50s. All calculations were performed by using f. nominal concentrations of both the test substance (as tested) and N2B03-3 , (as received) and the number of live or unaffected daphnids. The no* I observed effect concentrationT-is';? the highest concentration 'of itest* substance that did not cause, any ^toxicant-related mortality or sublethal" ^ .v.'-'1''//.S' effects. . . . . ,v \ ` ^ ,,k. M m m M M % 1 . *V . ; i m m x a A sf.'fS ; '.V ' Psge 12 of/25t)%'*V ... . fi# B . , .. bestcopy m nm XX. RE8ULT8 Ho insoluble material was notsd during the tsst. Biological and watar quality data ganaratad by tba acuta toxicity tast ara presented in Tabla 3 and Appendix A, respectively. During the teat with N2B03-3 the *5 neaaured concentrationa of diasolved oxygen ranged from 8.7 to 8.8 mg/L, the temperature ranged from 19.4 to 20.7'C, conductivity ranged from 570 to (10 pmhoa/cm, and pH ranged from 8.3 to 8.5. During the test with iaopropanol the measured concantrations of diasolved oxygen ranged from 8.1 to 8.5 mg/L, the temperature ranged from 20.4 to 20.9*C, conductivity ranged from 530 to 540 pmhos/cm, and pH ranged from 8.3 to 8.5. one hundred percent survival occurred in the control exposures and these daphnids did not exhibit any sublethal effects. During the toxicity tast with 390 mg/L iaopropanol no mortality or sublethal affects were observed and the 48 hour EC50 and LC50 values ara greater than 390 mg/L (Table 4). The 24 and 48 hour LC50a and EC50s for daphnids exposed to N2803-3 are presented in Table 5. Exposure of daphnids to N2803-3 resulted in a 24 hour median lethal concentration (LC50) of 500 mg/L N2803-3, a 48 hour LC50 of 400 mg/L H2803-3, a 24 hour siedian effective concentration (EC50) of 420 mg/L, and a 48 hour EC50 of 360 mg/L N2803-3, based on the N2803-3 concentration in the test substance. The 48 hour no observed effect concentration (NOEC) is 180 mg/L, based on the N2803-3 concentration in the test substance. The 24 hour LC50 is 1,000 mg/L, the 48 hour LC50 is 800 mg/L, the 24 hour EC50 is 840 mg/L, the 48 hour EC50 is 720 mg/L, and the 48 hour HOEC is 360 mg/L, based on test substance as-tested (50% N2803-3 and 50% iaopropanol). The slope of the 48 hour.dose response curve (based on mortality and immobilization) is 10.5. s' 8 *' m m tm > fPage 13.of 25.- -my>- <Mr 004009 xsrcopf aMfc`tr-. -. Tbl* 3. survival and aublethal effect data fro tha toxicity taat with daphnida, Daphnla magna, and N2803-3. nominal of N2803-3 (9/L) Rep. Number aliva 0 24 48 hr hr hr 0 (control) 65 ' no iso 300 500 1 10 10 2 11 10 1 10 10 2 10 10 1 10 10 2 10 10 1 10 10 2 10 10 1 10 . 9 2 10 : 9 1 10 2 10 5 5 10 10 10 10 10 10 10 10 8 9 2 2 Rota: Affected daphnida were immobilized. Number affected 0 24 48 hr hr hr 000 000 000 000 00 0 000 000 000 000 000 03 2 022 jrtf BEST COPY AVA1I & $ I COPY AVAH ABLE 004014 trtopjf KTCOPyj The protocol waa amended to pacify tha nominal concantrationa for tha definitive taat, to indicata that tha teat aubatance waa comblnad in a S0>50 ratio with iaopropanol, and to nota that a toxicity taat would also bo conductad with a aingle concentration of iaopropanol. PROTOCOL DEVIATIONS! The nominal iaopropanol concentration waa 390 mg/L rather than 500 mg/L. Thia deviation did not affect the outcome of the teat and no other daviationa were made from tha protocol. f i t W F Y A V AllABLE BEST COPY AVAU ftBLi Table h. 1. Dissolved oxygsn concentration, conductivity, pa, and temperature measured during tha toxicity test with daphnida, Daphnia magna, and N2803-3. Nominal Concentration of N2803-3 (mg/L) 0 (control) 65 110 180 300 500 Dissolved Oxygen (mg/L) 0 24 48 Rap. hr hr * hr Conductivity (pmhoa/cm) 0 24 hr hr 48 hr 1 8.7 8.8 8.7 2 8.7 8.8 .8.7 590 600 610 590 600 610 1 8.7 8.8 "8.7 ' ; 590 600 610 2 8.7 8.8 8.7 590 600 610 1 8.7 8.8 8.7 2 8.7 8.8 8.7 590 600 610 590 600 610 1 8.7 8.8 ' 8.7 I2 8.7 8.8 8.7 1 8.7 8.8 , 8.7 2 8.7 8.8 " 8.7 590 600 600 590 600 600 580 590 600 580 590 600 L 8.7 8.8 ; 8.7 2 8.7 8.8 8.7 570 590 590 . 570 590 590 ' \ v J`i' .V.,- Tabi* A.l. , continuad . i >* i cl" Nominai . Concontration of N2803-3 (ag/L) Rop. 0 br pH 24 hr 48 hr paratura 0 24 hr ' hr 48 hr . , rv, 'f ?V V 0 (control) 1 8.0 7.9 8.4 20.2 19.6 19.5 2 8.0 7.9 8.4 20.2 19.6 19.5 65 ,.;'V no 1 7.8 7.9 8.4 2 7.8 7.9 8.4 1 7.7 7.8 8.4 2 7.7 7.8 8.4 20.2 20.2 20.2 20.2 19.5 -19.5 19.6 ' 19.5 19.6 19.6 19.5 19.6 . 180 . - 1 7.6 7.7 8.3 2 7.6 7.7 8.3 20.1 ; 19.6 ' *19.5 '' 20.1 '19.5 19.4 300 1 7.6 7.7 8.2 20.1 19.6 19.4 / 2 7.6 7.7 8.2 20.1 . 19.6 19.4 !. > , 500 1 7.5 7.6 B.O 20.2 19.6 19.4 2 7.5 7.6 7.9 ** 20.2 > 19.5 19.4 , * Table A.2. Dissolved oxygen concentration, conductivity, pH, and temperature measured during the toxicity test with daphnids, : Daphnla jugna, and isopropanol. / sjv*- Nominal Concentration of.Isopropanol (mg/L) Rep. Dissolved Oxygen (mg/L} ---------- ------------- 0 24 48 hr hr ,... hr .: Conductivity (pmhos/cm) ------------------- - 0 24 48 hr hr hr 0 (control) 390 1 8.1 8.5 8.4 2 8.1 8.5 8.4 1 8.1 8.5 2 8.1 8.5 8.4 540 530 530 540 530 S30 ' 540 530 530 540 530 530 BiST COPY AVftllABLE Tabi A. 2. continuad. Temperatura (*C) 0 24 48 hr he hr 0 (control) 390 1 r.s 8.4 8.3 2 8.5 8.4 8.3 20.4 20.4 20.9 20.8 20.6 20.6 1 8.5 8.4 8.3 ' . T '' 20.4 20.8 20.6 2 8.5 8.4 8.3 20.4 20.9 20.6 A i Ki 1 ,< V ** `jv .v.w K" , '.' -'..'`.4r.-r.*-.v'..**r'--.iVi.t.JVVAy-i*,r-.jk<**.'.i-',V , -.O<s,.1 d 8L& 1 **i * 1*t.f i r * v cfr^i \ { ; * V '4 Y - - - * :A X r '\ f^ rz w ^ -3 ' v i i V./*3S,,' a&i vr-j/x