Document qd8Od0ddRBa2RkkR3kbLoJqnE

M AS 6 -ZHS ACUTE TO XIC ITY TO AQUATIC INVERTEBRATES (ACARTIA TONSA) TEST SUBSTANCE_____________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-602. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was not noted. The test sample is FC-602. Current information indicates it is a mixture of 1% PFOS, 12% diethylene glycol butyl ether, 61.05% water, 20% ethylene glycol, 2% Sultone foamer, 1% sodium octyl sulfate, 1% sodium decyl sulfate, 1% magnesium sulfate, 0.7% xanthan gum, 0.1% starch, 0.1% n-(3-chloroallyl)hexaminium chloride, and 0.05% tolytriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. METHOD:____________________________ ___________________________ M ethod: ISO/DIS 14669-1997: W ater quality - Determination of acute lethal toxicity to marine copepods (Copepoda, Crustacea). Type: Acute static GLP: No Y ear com pleted: 2000 Species: Acartia tonsa Supplier: Test organisms cultivated from eggs, supplied by Marine Biological Laboratory, University of Copenhagen, Denmark. A nalytical m onitoring: Dissolved oxygen, and pH Exposure period: 48-hours T est organism age: Large copepodites and adults. S tatistical m ethod: LC50 values calculated by PROBIT Analysis, National Swedish Environmental Protection Board (Version 2.3,1990). Calculation of NOEC is by single factor ANOVA followed by Bonferronis procedure. Test conditions: Dilution w ater: Filtered natural seawater (34 ppt salinity) from an unpolluted site in Byfjord, Norway. Dilution w ater chem istry: Not given. Lighting: A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. Stock and test solution preparation: A 1000 mg/L primary stock solution was prepared by diluting 0.9998 g of test substance in 1 L of water. Aliquots were then added directly to water in test vessels to create test solutions. Exposure vessels: 28 mL rolled rim vials containing 25 mL of test solution 002125 Num ber o f replicates: 4 Num ber o f organism s per replicate: 5 Num ber o f concentrations: five plus a blank control W ater chem istry during the study: pH range (0-48 hours): 8.2 - 8.2 (control exposure) 8.0 - 8.2 (1000 mg/L exposure) Tem perature range: 20 1 C Dissolved oxygen range (0-48 hours): 7.3 - 7.4 mg/L (control exposure) 5.7 - 7.4 mg/L (1000 mg/L exposure) RESULTS__________________________________ ______________________ Nom inal concentrations: Bk control, 10, 3 2 ,10_0, 320, and 1000 mg/L. Reference substance (3,5-dichlorophenol) at 1.0 mg/L Elem ent values: 24-hour LC50 = 350 (250 - 490) mg/L 48-hour LC50 = 200 (140 - 270) mg/L 48-hour NOEC = 100 mg/L Element values based on nominal concentrations Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS__________________________________ _________________ The FC-602 48-hour LC50was determined to be 200 mg/L with a 95% confidence interval of 140 to 270 mg/L. The 48-hour No Observed Effect Concentration (NOEC) was 100 mg/L. Validity criteria fulfilled. Reference substance in normal range. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, the study lacks information on purity of the test substance and it lacks analytical confirmation of the amount of fluorochemical proportion^ in the solution. REFERENCES______________________ _____________________________ 002126 Test was conducted by RF Rogaland Research, of Stavanger, Norway at the request of 3M Norge, Lab reference number 99312-3, 2000. OTHER______________________________________________________ ___ Last changed: 6/26/00 002127 L9s9u3u1.r2e-/3.no,; Page I o f31 IS ) NORSK AKKREDfTERiNG ROGALAND RESEARCH "FC-602 Light Water Brand ATC plus" Toxicity tests Report RF-2000/003 Lab. ref. no.: 99312-3 No. of pages: 31 ISBN: Author: Elin Horve Client(s): 3M Norge Distribution restriction: Version No. / date: Vers. 1 / 07.01 2000 Research Program: Open from (date): Summary: The following test results were obtained with "FC-602 Light Water Brand ATC plus": Toxicity: Skeletonema costatum Acartia tonsa Corophium volutator 72 h EC* 48 h LCM 10 d LCM (mg/l): -78 (mg/l): 200 >3070(mg/kg dry weight): The results relate to the product as supplied by Novatech. Key-words: Toxicity, Skeletonema costatum, Acartia tonsa, Corophium volutator. No part ot this document may be reproduced in any form without permission in writing by RF Rogaland Research . -/ 4 a . (Study director J1 Inger Lisa Andersen 0 0 z z 8for RF Rogaland Research Kre Netland ANGER; ProfessorO iciv HonssensveBi 1E5R,GPE.ON'.IBtovxv2m50r3iiUUO^anMdhaWug,W4091 Stavwon--gs~r,~NoXrwJ ay*..ATtel.: +k4a7 -5s1o 875e--0 00^.aFtax: +s4a7 t5o1 A8n752 00. Enterprise number.: 950884800. L9a9b3.1r2e-f3.no.: Page 2 o f31 CONTENTS: 1. Preface................................................................................................................................................. 3 2. TEST FOR TOXICITY WITH SKELETONEMA COSTATUM......................... 4 3. Test for toxicity with acartia tonsa.......................... r.................................................................... 1o 4. Test for toxicity with Corophium volutator................................................................................ 15 5. Data sheet(s).................... 20 L99ab3.1r2e-f3.no.- Page 3 o f31 PREFACE; NEcoovtaotxeiccho.loTghiceaslatmesptlsewiseraecpoenrcfoernmtreadteotnha"Ft Cn-o6r0m2 aLlilgyhwt Willatbeer uBsreanddinATaC3%pluAs"F in agreement FF/6% AR with solution. Tests were conducted in accordance with the "Harmonised Offshore Chemical Notification Format" (OSPARCOM1995/S F T 1998). pTehrefoprrmojeedctbwyaRsFca- Mrriieljdeloaubt.aSt tRudFy-dRiroegcatolarnwdaRseSseenairocrhEfonrgi3nMeeNr Ionrggeer. LAisllatAhendteesrtssewn.ere bTyheNtoevsattreecshu.lts arepresented in separate sections. The results relate to theproduct as supplied This test report is issued by a laboratory accredited by Norwegian Accreditation (NA). The accreditation states that the laboratory meets the NA requirements concerning competence, Ietqaulispomsetantteasntdhawtotrhkeinlagbporroacteodruyrheassfoarsaatlilstfhaecttoersyt mquetahloitdysacsosunrtaainnceedsiynsttehme,awcchriecdhitiantcilound.es internal quality controlschemes, participation in interlaboratory comparisons, and the use o f certified reference materials and secondary reference materials. The testsperformed are covered by the accreditation. wThriistintegstbryeRpoFr-t mMailyjenlaobt .be reproduced in anyform other than infull, without thepermission in 002130 L99a3b1.r2e-f3.no. Page 4 o f31 T est for T oxicity Marine alga: Skeletonema costatum 002131 L99a3b1.r2e-f3.no Page 5 of31 TEST REPORT Grow alga tShkienlheitbointieomn taecstoswtaitthuttnh e m a r in e (0 ) NORSK AKKREDITERING . pu ROGALAND RESEARCH TEST SUBSTANCE: "FC -602 Light W ater Brand ATC plus" TEST RESULT: The 72 hours ECjowas calculated to ~78 mg/1. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: NwSit-hESNkeIlSeOton1e0m25a3c,o1s9ta9t8u:mWaantderPqhuaaeloitdya-cMtylaurminetraiclgoaml ugtruomw.th inhibition test TEST ORGANISM: Skeletonema costatum, strain NIVA BAC 1. A stock culture of the test ^ organism is kept at RF - Rogaland Research. PREPARATIONS: Natural seawater (salinity: 34 %o) from an unpolluted site in Byfjord was used as basis for the growth medium. The water was filtered through a GF/C filter,, and briefly heated to about nCuotmriemntisssaiocnco. rding to ISO 17052C53p, rainodr to use. The seawater was enriched recommendations from the Paris with ` ^' Test solutions were prepared from a stock solution. A stock solution o f 102.4 mmge/d1iuwmas. pOrethpearrecdonbcyensttirrartiinogn0s.w10e2re4pgrreapmarseodfftrhoemprthodisuscttoicnk1solilturteioonf.growth INCUBATION: Test cultures o f 50 ml were incubated in 100 ml flat-bottomed flasks on an orbital shaker, 3 replicates for each concentration. Test cultures were inocu Tlahteedtetostawbaosutru2n00u0ndceerllsc/omntlinfruoomusanligehxtp(o>ne5n0tipaElly/ngrr-soewki)nfgroinmocfululuomresccuelntut re. tubes o f universal natural white type, at a temperature of 20 2C. Tthheetetessttfwlaaskssruenacfhordaabyofuotr7g2rohwotuhrsm, aenasdusruebmseanmtsp.lAeslgoafl5gmrolwwtherweataskmeneafsruormed by fluorescence. pH measurements were performed as indicated on data sheet. ANGER: Professor Olay Hanssensv 15. PO Box 2503 Ullondhqug. 4091 Stavanaer. Norwav W * A 7 i ~ 002132 L9a9b3.1r2e-f3.no. Page 6 o f31 DATA ANALYSIS: Calculations: Growth rates were calculated by log linear regression on biomass (fluorescence) versus time, as described in NS-EN ISO 10253. ErC50: Tceofhfneeccietnnchtoirbnaicttieoonrnytroeafftfiaoepnctrwoodanustcchtaeglcgivuriolnawtgethd50bo%yf Sirnkeetdeluertpcotoniloaentmiooanfcgboresottwwatteuhemnra3itse2em(xEgprC/rle5as0ns).eddTahsisthe 102.4mg/l.. NOEC: CfaaclctourlaAtiNonOoVfANOfoEllCow(NedobOybBseornvfeedrrEonffiescptrCocoendcuenretrfaotriocno)mispdaroinnegbeyacshingleconcentration mean with the control mean. TEST RESULTS: Table 1. EC and NOEC values obtained with Skeletonema costatum. Test substance "FC-602 Light Water Brand ATC plus" ErCio - EfCso ~78 ErCo >102.4 Comments: Validation: NOEC: 32 GVVaarllauupeehssPainarddeiIccnaaSltcetauctloa3nt.e0cde. nbtyraintitoenrspogliavtiinogn.10N%O. E50C%araencda9lc0u%laitnedhibbyitiuosninogftghreocwotmh prauttee.rpUrongitrsaimn mmeg/l. The results are based on growth rates for the time span 0 - 72 h, the period d(suereinggrowwhtihchcuthrveegsr).owth is considered to be exponential in the control cultures Tcehlel dveanlisditityyicnrcirteeraisaedgibvyenaifnacthtoertoesftmporroetotchoalnar1e6fiunltfhileletde,sitnptehraiotdthe control v(3ca,o5rr-ydrbeicsyhpmloonordoriepnhtghetanonoal1wg,0raouswnintihtsthrdaeutreraionngfgmethnoeroteremtshtaaalnlnyd0f,ot9huden'd71).2, thhoeucrosntetrsot lrpesHuldtsidwnitoht Lab. ref. no.: TRSaeemsptopprlteerdriaoetdcee::ived.: 99312-3 2027-22.0051..01.2910..990990 Enclosed: Datasheets Elin Horve Engineer Analytical technician SenQiAourgaRelieMtyseoAalvrsceshursrSmacnyicerentist Tfhoirsmtoetshterreptohratniisnifsuslul,edwibtyhoaultatbhoerapteorrmyaiscsciroendiintedwrbiytinNgobrwy eRgFia-nMAiclcjraeldaibt.ation(NA). The reportmay notbereproduced inany 002133 L9a9b3.1r2e-f3.no.- Page 7 of31 PRIMARY DATA: Growth inhibition test with Skeletonema costatum Test substance: "FC-602 Light Water Brand ATC plus" Lab-ref.: 99312-3 Start date: 22.11.99 Cone, unit: mg/l Measurement: The biomass was measured by fluorescence; values in fluorescence units Time: 22.11.99 23.11.99 24.11.99 25.11.99 Concentration 13:40 11:50 13:20 14:45 Growth pH pH rate Corre at start at end day -1 lation of test of test 0 0.06 0.32 1.5 8.2 1.61 0.999 0 0.06 0.23 1.7 9.3 1.66 0.999 0 0.06 0.46 3 11 1.71 0.992 8.4 8.3 0 0.07 0.46 2.15 11 1.66 0.997 0 0.07 0.35 1.9 9 1.59 0.999 0 0.12 0.4 2.35 11.5 1.50 0.999 0.32 0.07 0.42 2.4 11.5 0.32 0.09 0.55 2.5 12.5 0.32 0.07 0.5 2.65 12 1.68 0.998 1.62 0.998 1.69 0.996 8.4 8.2 1 0.08 0.66 3.4 14.5 1.71 0.993 1 0.07 0.64 3 13 1.72 0.991 8.4 8.2 1 0.09 0.5 2.7 12.5 1.62 0.999 3.2 0.08 0.58 3.4 15 3.2 0.09 0.45 3 13 3.2 0.09 0.38 2.95 13 1.72 0.996 1.63 0.998 1.63 0.998 8.4 8.2 10 0.1 0.35 3.1 15 1.65 0.997 10 0.1 0.43 3.1 14.5 1.63 0.999 8.4 8.2 10 0.07 0.43 2.6 14 1.74 0.999 32 32 32 102.4 102.4 102.4 0.09 0.5 2.7 14.5 0.08- 0.46 2.1 11 0.09 0.43 2.9 13 20.1 0.13 0 0.43 0.07 0.08 0.18 0.43 0.09 0.1 0.2 0.73 1.67 1.62 1.63 0.48 0.60 0.69 0.999 0.998 0.999 0.979 0.971 0.944 8.4 8.4 8.3 8.3 Reference substa nee (3,5-dk:hlofODhenol): 1.5 mg/l 0.1 0.16 0.48 1.5 mg/l 0.07 0.18 0.44 1.5 nriQ/l 0.1 0.15 0.38 Mean values: 0 0.32 1 3.2 10 32 102.4 0.07 0.08 0.08 0.09 0.09 0.09 0.09 Reference subst. 0.09 0.4 0.5 0.6 0.5 0.4 0.5 0.1 0.16 2 3 3 3 3 3 0 0.4 1.1 1.4 1.15 10 12 13 14 15 13 1 1.2 0.79 0.98 0.80 0.993 0.999 0.987 8.3 8.2 1.62 0.999 1.66 0.997 1.68 0.995 1.66 1.67 1.64 0.998 0.999 0.999 The result ol the reference substance (53% 0.59 0.964 inhibition) is within the range 0.86 0.996 (2o-8o%),oundO213 4 L99a3b1.r2e-f3.no.: Page 8 of31 GROWTH CURVES: Growth Inhibition test with Skeletonema costatum I Test substance: "FC-602 Ught Water Brand ATC plus" Lab. ref.: 99312-3 I 1 002135 L9a9b3.1r2e-f3.no.: Page 9 o f31 Inhibition of the growth of Skeletonema costatum with: mg/1 (FC-602 Light Water Brand ATC plus) Experimental data: Concentration in Growth rate FC-602 Light Water Brand ATC plus Control 1 Control 2 Control 3 Control 4 Control 5 Control 6 Control mean : 0.32 0.32 0.32 1.00 1.00 1.00 3.20 3.20 3.20 10.00 10.00 10.00 32.00 32.00 32.00 102.40 102.40 102.40 1.61 1.66 1.71 1.66 1.59 1.50 1.62 1.68 1.62 1.69 1.71 1.72 1.62 1.72 1.63 1.63 1.65 1.63 1.74 1.67 1.62 1.63 0.48 0.60 0.69 Inhibition in percent 0 0.0 0.1 0.0 000...010 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.1 0.0 70.4 63.0 57.5 002136 L99a3b1.r2e-f3.no Page 0 o f31 '' t. M Hi (' -i*. T est for T oxicity Marine herbivore:\ O'-'r`..H.V \>y,_VV:. Acartia tonsa 002137 L99a3b1.r2e-f3.no.: Page 11 o f 31 TEST REPORT ACUTE TOXICITY TEST WITH THE MARINE COPEPOD A c a V titt tOHStt ! (S i NORSK AKKREDITERING Nr.POI ROGALAND RESEARCH TEST SUBSTANCE: "FC-602 Light W ater Brand ATC plus" TEST RESULT: The 48 hours LC50was calculated to 200 mg/1. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ItSoOm/DarIiSne1c4o6p6e9p-od19s9(7C:oWpeaptoedraq,uCalriutyst-acDeeat)e.rmination o f acute lethal toxicity TEST ORGANISM: AUcnaivrteirasittoynosaf C(Doapnenah),asguepnp. lied by the Marine Biological Laboratory, Cultivation: The test organisms were supplied as eggs, and were cultivated in accordance with the "Paris Commission - Ring Test Protocol" (VKI). Large copepodites and adults were used in the test. PREPARATIONS: Natural seawater (salinity: 34 %o) from an unpolluted site in Byfjord was used as basis for the dilution water. The water was filtered through a GF/C filter prior to use. Test solutions were prepared from a stock solution. A stock solution o f 1000 mg/1 was prepared by stirring 0.9998 grams of the product in 1 litre o f dilution water. Other concentrations were prepared from this stock solution. INCUBATION: The test was run in glass containers (28 ml rolled rim vials) each containing 25 ml of test solution and 5 animals, 4 replicates for each concentration. The- v2di0aatlas sw1heeCreet.. kpeHptaunnddoerxyag1e6nhm/8ehalsiughret/mdaenrktspwheorteoppeerrifoodrmatead temperature as indicated of on the 002138 L9a9b3.1r2e-f3.no.: Page 12 o f31 DATA ANALYSIS: CALCULATIONS: The effect on the survival o fAcartia tonsa is expressed as the concentration of paeprrtoedsut cctolnecthenatlrtaoti5o0n%weorfethuesetdesftoarnciamlcaulsla(tLioCn5.0)T. hTeh2e4chomoubrisnaenddm4o8rhtaoluitrys LstCan50davradlupersocaenddutrheeP9R5%OBcIoTnAfidneanlycseisli,mNiat twioenrael cSawlceudlaisthedEbnyviurosinnmg etnhteal Protection Board (Version 2.3,1990). This programme also calculate test concentrations lethal to 10% and 90% of the test animals. NOEC: CfcaoacnltccouerlnaAttriNaotnOiooVnfAmNfOeoaElnloCww(ietNhdotbhOyebBcsooennrvtfreeordrl oEmnfeifasencp.t_rCocoendcuernetrfoartiocnom) ipsadroinnge beaychsingle Test RESULTS: O O Table 1. LC and NOEC values obtained with "FC-602 Light Water Brand ATC plus" Acartia tonsa 24 hours 48 hours LC10 120 (56-190) 87 (42-130) 350 (250-490) 200 (140- 270) LC90 970 (650 - 2200) 450 (320 - 900) NOEC: 100 100 ctVhaaellcucueolsamitnepdudtibecyraptPerRo1gO0r%BamI,T5m0ae%naClayrnsadips,h9Pa0%naddl9Ien5thS%atalcittoy3n.a0fif.dteern2ce4ianntedr4va8lhsoaurres.indUicnaittsedin. mNgO/lE. CVaarlueecsaalcreulated by VALIDATION: The validity criteria given in the test protocol are fulfilled, in that the dissolved oxygen concentration at the end o f the test was greater than or equal to 4 mg/l, the percentage mortality in the controls was less than 10%, and die 48 hours LC50of 3,5-dichlorophenol (reference) was in the range normally found. LTSaaembst.pprleeefr.rionedco:e.:ived.: Report date: 22-229059..3011912..99-399 07.01.2000 Enclosed: Datasheets EElinngHinoerevre Analytical technician SenQiAourgaRelieMtyseoAalvrsceshrusrSmacnyiecren--tist Thistestreportisissuedby a laboratoryaccreditedby Norwegian Accreditation(NA). The reportmay notbereproduced inany form otherthaninfull,withoutthepermissioninwritingby RF -Miljolab. 002139 L9a9b3.1r2e-f3.no.: Page 13 o f31 PRIMARY DATA: Acute to xicity te s t w ith Acartia tonsa Test substance: "FC-602 Light W ater Brand ATC plus" Lab. ref.: Start date: Measurement: 99312-3 10.11.99 Number of dead Concentration (mg/1) Control 10 32 100 320 1000 Total number Number of specimen pr. vessel Specimen A C E pr. cone. B D F 556 30 4 5 5 5 55 20 5 5 55 20 5 5 55 20 5 6 54 20 5 5 64 20 5 NUMBER OF DEAD 24 hours ACE B DF Total 48 hours ACE BDF Total 0 00 0 00 100 0 001 0 000 100 10 1 1 0 00 0 02 0 00 2 0 10 020 1 21 3 4 10 2 5 54 5 443 74 5 64 19 5 15 20 Reference substance (3,5-dichlorophenol): 1.0 mg/l 5 55 1 10 331 30 6 4 5 o o 3 0 11 The result with the reference substance is within the range normally found. 9 002140 L9a9b3.1r2e-f3.no.: Page 14 o f31 Test substance: "FC-602 Light Water Brand ATC plus" Lab. ref.: Start date: 99312-3 10.11.99 Measurements o f oxygen and pH. Start 24 hours 4 hours Concentration (mg/l) Oxygen (mg/l) pH Oxygen pH Oxygen (mg/l) (mg/l) Control 10 7.3 8.2 7.6 8.2 ^ . 7.4 7.3 32 7.6 8.2 7.2 100 7.6 8.2 7.1 320 7.6 8.2 6.7 1000 7.4 8.2 5.7 pH 8.2 8.2 8.2 8.2 8.1 8.0 Reference substance (3,5-dichlorophenol): 1.0 mg/l 7.3 8.2 7.4 8.2 L99a3b1.r2e-f3.no.: Page 15 of31 T est for toxicity Marine Amphipode: Corophium volutator 002142 L99a3b1.r2e-f3.no.: Page 16 o f 31 TEST REPORT ACUTE TOXICITY m arine am phipo SdEDCIMorEoNpThTEiuSTmWvIToHluTHtaEtor (3 ) NORSK AKKREOITERING Nr.POIS ROGALAND RESEARCH TEST SUBSTANCE: "FC-602 Light W ater Brand ATC plus" TEST RESULT: The lOd LC50was calculated to >3070 mg kg' 1dry weight sediment. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ATSoxTiMcityET13e6st7s-9w0it,hStManadrainrde aGnudidEestFuoarriCneonSdeudcimtinegnt1s.0 day Static Sediment TESTorganism: Corophium volutator, from an unpolluted site near Trondheim. CULTIVATION: The test organisms were supplied as adults (4-8 mm in size) and were held in afeeerdatinedg.seawater at 15C. A small amount of natural seaweed was used for PREPARATION: NStaatvuarnagl eserawwaasteurse(sdalfionritdyi:lu3t4io%now) afrtoerm. aSneduinmpeonlltucteodllescitteedinaBt aynfjourndpeonlluoutetdsidsiete was used for preparing test sediments. The sediment is a muddy sand and was analysed for organic content and proportion silt for each test. Each test concentration was prepared by adding the product in 600 grams of dbaumckpetsewdaims sehnatkinenafporla3sthicoburusc.kTeth. e2s0e0dimmleonft seawater was added and die was divided into three beakers, 4n0ig0hmt. l of 10 seawater was animals were added to each, then randomly and the beakers were aerated over selected for each beaker. A sample o f cthaelcdualamtepdsaeds immgenktgw' 1assedainmaelynsteddrfyorwweiagthetr. content, and all concentrations are INCUBATION: The test was run in glass containers (600 ml) each containing 200 g o f test sceodnicmenetnrta,taiopnp.. 4T0h0embelaokfesresawwearteerkaenpdt u1n0daenricmonaltsin, 3uoruesplaicearatetisofnorateaach tinemdipceartaedturoenothfe15data1shCe.etp.H and oxygen measurements were performed as 002143 ^SGR: Professor Ofav Hanssensvei 15, P.O. Box 2503 Ullandhaog, 4091 Stavanger, Norway. Tel.: +47 51 8 7 5 0 00 . Fax: +47 51 8 7 52 00 . Enterprise number.: 950884800. ______ ____________________ BERGEN: Thormehlensgt. 5 5 .5 0 0 8 Bergen. Norway. Tel.: + 47 55 54 38 50. Fax: + 47 55 54 38 0 L99a3b1.r2e-f3.no.: Page 17 o f31 DATA ANALYSIS: CALCULATIONS: The effect on the survival o f Corophium volutator is expressed as the concen mvtraaolturioteanaliontydf patheprerto9ed5s%tucctocnloecntehfnaidtlretanotci5oe0nl%iwmeoirtfeatrhueesectdaeslfctouarlnacitameldcaublslya(tLuiosCnin5.og)T.thhTeehs1et0acndodamayrbdLinCe5d0 PROBIT Analysis, National Swedish Environmental Protection Board (Version 2.3,1990). This programme also calculate test concentrations lethal to 10% and 90% of the test animals. NOEC: Calculation of NOEC (No Observed Effect Concentration) is done by single fcaocntcoernAtrNatOioVnAmfeoalnlowwiethd tbhyeBcoonntfreorlromneisanp.rocedure for comparing each Test RESULTS: Table 1. LC and NOEC values obtained with "FC-602 Light Water Brand ATCplus". Corophium volutator 1 0 days LCjo 1750 LC5o >3070 LC90 >3070 NOEC: 3070 GVVaaralluupeehssPainarddeiIccnaaStlectau1tl0a3%t.e0,d.5b0y%PRanOdB9I0T%anlaetlhysailsi.tyNaOfteErC0ardeacyasl.cuUlantitesdinbymthgekcgo'1msepduitmerepnrtodgrryawmemigeht. VALIDATION: The validity criteria given in the test protocol are fulfilled, in that the dissolved otFrihsexleucyfooopgmureeanrnmncdtceeetonnonntdeabcege(edrenwehtmfrieiatgorhtheriiontneancrlaei.atc)ytcwteihnpaestteheidnnedlctihmooenfittrtrsahonfelogstreewsonetrorgwreamanlseiascglslrcyetohafnoatteunernn1tdth5.b%aunTtahotnrehdeesqeasduitliaetmlsfttreoawnct6itt0uihos%nedi,s Table 2. Characteristics of the sediment used for testing Organic content (%) 0.7 Silt fraction (%) 0 .8 Lab. ref. no.: TSRaeemsptopprleterdrioaetdce:e:ived.: 2909.30192.9-39 03-12.12.99 07.01.2000 Enclosed: Data sheets Elin Horve Age Molversmyr AnalyEticnaglinteecehrnician SenQiouraRlietyseAarscshurSacniceentist Thistestreportisissuedby alaboratoryaccreditedbyNorwegian Accreditation(NA). The reportmay notbereproduced inany form otherthaninfull,withoutthepermissioninwritingby RF -Miljalab. 002144 L9a9b31.r2e-f3.no.: Page 18 o f31 PRIMARY DATA: Acute toxicity test with Corophium volutator Test substance: "FC-602 Light Water Brand ATC plus" Lab. ref.: Start date: Measurement: 99312-3 03.12.99 Number of dead Total number Number of specimen pr. vessel Concentration Specimen A C E (mg/kg dry weight) pr. cone. B D F Control 10 10 10 60 10 10 10 10 10 10 257 30 10 10 10 478 30 1050 10 10 10 30 3070 10 10 10 30 NUMBER OF DEAD 5 days 10 days ACE BDF Total A C BDF Total '0 0 0 0 00 0 100 1 0 10 1 3 10 4 201 3 Reference substance (fluoranthene): 20.3 10 10 10 30 3 22 The result with the reference substance is within the range normally found. 7 002145 L99a3b1.r2e-f3.no.: Page 19 of31 Test substance: "FC-602 Light Water Brand ATC plus" Lab. ref.: Start date: 99312-3 03.12.99 Measurements o f oxygen and pH. Start Concentration Oxygen (mg/kg dry weight) (%) pH 5 days Oxygen (%) pH Control 94 8.1 91 8.1 257 93 8.1 92 8.1 478 93 8.1 91 8.1 1050 95 8.1 92 8.0 3070 96 8.1 88 7.9 10 days Oxygen (%) pH 91 8.0 82 8.1 81 8.1 90 8.1 89 8.1 Reference substance (fluoranthene): 20.3 95 8.1 90 8.1 90 8.1 I 002146 Data Sheets 002147 99312-3 HMS-DATA3LAD (Heise-, Miljo- og Sikkerhetsdatablad) Dokumentr.: Erstatter : 07-2573-9 Utgitt : 10/11/93 Versjon : 05.00 08/10/97 Skrevet ut : 11/11/98 Side 1 av n HMS-datablacet er utarbeidet av Miljeavdelingen ved 3M Norge A/S. Telefon: 63 84 75 00 / Telefax: 63 84 17 88 1 IDENTIFIERSJON AV KJEMIXALIST OG ANSVARLIG FIRMA. Importer: 3M Norge A/S Hnde lsr.avr. : FC-201A? LIGHT WATER (TM) 1% Ar FF (FREEZE PROTECTED) Deklaras jor.snummer, Produktregister et : Telefon, Giftinformasjonssentralen: 22 59 13 00 2 OPPLYSNINGZR OM KJEMISX SAMMENSETNING. Informasjon om enkeltkompcnenter, faresymbol(er) og risikosetninger STOFFNAVN CAS-nr VSKT-PROSENT VANN 7732-18-5 2-(2-3UTOESYZTOESY)-ETANOL / 112-34-5 (DIETYLZNGLYXOLMONOBUTYLETSR) Faresymboler/risikosetninger: Xi; R:36 1,2-ETANDIOL 107-21-1 Faresymboler/risikosetninger: Xn; R:22 AMFOTER FLUOROALXYLAMID-DERIVAT Trade Secret ALKYL-SULFAT-SALTER Trade Secret TRIETANQLAMIN 102-71-6 PERFLUOROALEYL-SULFONAT-SALTER Trade Secret RESTER AV ORGANISES FLUORKJEMIKALIER Trade Secret 35.0 - 39.0 17.0 - 21.0 * 14.0 5.0 - 10.0 14.0 - 16.0 SB 3.0 1 . 0 - 5.0 Ikke kjent 99312-3 FC-201AF LIGHT WATER (TM) It AFFF (FREEZE PROTECTED) 3 VIXTIGSTE FARSMOMENTER. Vikzigste skadevirkninger Helseiara: IRRITERENDE (Xi) Irrigerer a y n e n e . Sikkerhet: Miljafare: 4 F0RSTEKJSLPSTILTAX. Tiltak ayr.e: Skyll szraks ayner.e grundig med rikeliga mengder vann i minst 15 minutter. Konzakt lege umiddelbart. Skylling under transport. Tiltak hud:Skyll straks huder. med spe og rikelige mengder vann. Fjern tilsalt tay. Hvis symptomer oppstir, kontakt lege. Vask tilsalt toy for det brukes igjen og kst tilsalt skotay. Tiltak intending: Hvis symptomer oppstir, bring vedkommende bcrt fra eksponeringskilden. Sarg for frisk luft. Hvis Symptome ne vedvarer, kor.rakr lege. Tiltak svelging: Kontakt lege umiddelbart. Fremkall breknir.ger kun i samrid med medisinsk personell. Gi aldri noe gjennom munnen til en bevisstlos person. 5 TILTAK VED BRANNSLUKNING. Egnet brannslukningsmiddel: Produktet er et brannslukkingsmiddel. Ikke egnet brannslukningsmiddel: Eksponeringsrisiko ved brann: 002149 99312-3 FC-201AF LIGHT WATER (TM) U AFFF (FREEZE PROTECTED) Forbrenr.ingsprodukter ved brann: Prosecyrer ved slukking av brann: 6 TILTAX VED UTILSIKTST UTSLIPP. Personlige vernetiltak: Se vernetiltak under andre punkter i dette HMS-databladet. Tiitak ved utilsiktet utslipp: Ventiler omrdet. Spill avgrenses umiddelbart. Dekk til med et absorberende materiale. Spill samles ,opp umiddelbart. Rengjor omrdet med vann. Plasseres i en egnet beholder med lokk. Fjerning av kjemikalieavfall: Se pur.kt 13, Fjerning av kjemikalieavfall. 7 HNDTERING OC- 0PP3EVARING. Betingelser for sikker oppbevaring: Ml ikke lagres i omrider hvor produktet kan komme i kor.takt med mat eiler legemidier. Lagres ved temperatur under 49 grader celsius. Lagres ved. temperaturer over 0 grader Celsius. Hold beholceren tatt lukket n&r produktet ikke er i bruk. Uforenelige materialer: Ventilasjon: Oppbevar emballasjen pa et godt ventilert sted. Sikkerhetstiltak for k forhindre brann: Sikkerhetstiltak for k forhindre eksplosjon: Ikkebrennbar. Sikkerhetstiltak for k forhindre statisk elektrisitet: ForhoIdsregier ved bruk: Hold emballasjen tett lukket. Rayking forbudt: Under bruk av produktet kan reyking forrsake forurensning av tobakken og/eller rayken og farlige dekomponeringsprodukter kan dannes. Se punkt 10, stabilitet og reaktivitet, skadelige dekomponenter. c9a9o3.1r2e-j3.no.: I uge wj ->. FC-201AF LIGHT WATER (TM) 1% AFFF (FREEZE PROTECTED) 8 EXSPONERINGSKONTROLL OG PERSONLIG VSRNEUTSTYR. t Oyevern: Unr.gi i fk dan?, spray eller tike i cynene. Bruk tette vernebriller med ventiler. Hir.dvern: 3ruk egnede verr.ehar.sker av hensiktsmessig materiale ved hir.dtering av produkzet. Vernehansker av felgende materiale(r) anbefales: Butylgummi. Annet hudvern enn hindverr.: Unngi hudkbntakt. Andedrettsvern: Unr.gi inninding av luftbiret materiale. Basert pi konsentrasjonen av lufcforurensr.inger i arbeidsatmosfaren velges en avfelgende type (r ) godkjer.t indedrettsvern: halvmaske med filter mot orgar.iske damper og vaskeformige partikler: A/P2SL. Sveiging: Det mi ikke spises, drikkes eller reykes under arbeid med produktet. Vask utsatte omrider grundig med sipe og vann. Vask hendene etter hindtering og fer spising. Anbefalt ventilasjor.: Kvis utsugningsventilasjon ikke er tilstrekkelig, mi egnet godkjent indedrettsvern benyttes. Serg for tilstrekkelig ventilasjon slik at dampkonsentrasjonene holdes under anbefalte administrative normet og/eller kontroller spray eller tike. Adra. normer for forurensning i arbeidsatmosfare, Februar 1996: VANN (7732-18-5) Anmerkninger.: Ingen adm. norm. 2-(2-3UTOKSYETOKSYJ-ETANOL / (DISTYLENGLYXOLMONOBUTYLETER) (112-34-5) 8-timers verdi ppm: 35 Anmerkninger: Amerikansk grenseverdianbefalt av produsenten. (Ingen norsk adm. norm). 1/2-ETANDIOL (107-21-1) 8-timers verdi ppm: - (stav) 8-timers verdi m g / m 3 : 10 (stav) Takverdi ppm: 25 (damp) Takverdi mg/m3: - (damp) AMFOTER FLUOROALKYLAMID-DERIVAT (Trade Secret) Anmerkninger: Ingen adm. norm. ALKYL-SULFAT-SALTER (Trade Secret) Anmerkninger: Ingen adm. norm. j ! j i ' t ]i j ; i ; j ij t | ! | ! t j | j j i | j ; j j 99312-3 FC-201AF LIGHT WATER (TM) 1% AFFF (FREEZE PROTECTED) ----- rrer--r.v;oiAM:i ( --------------------------------------------- - 8-timers verdi ppm: - 8-timers verdi mg/ra3: 5 PEXFLUOROALKYL-SULFONAT-SALTSA (Trade Secrec) 3-timers verdi m g / m 3 : 0,1 Anmerkninger: Amerikansk grenseverdi anbefalt av produsenten. (Ingen norsk adra. norm). RESTER AV ORGANISXS FLUORXJEMIKALIER (Trade Secret) 8-timers verdi mg/m3: 0,1 Grenseverdi anbefalt av 3M. 9 FYSISXE OG XJEMISXS EGENSKAPER. Tilstandsform, farge, lukt: PH: Xlar ravfarget vaske. 7.5 - 8.5 Xokepunkt/-intervail: 100 C (Segynnende kokepunkt) Smeltepunkt/-intervall: Ikka relevant Flammepunkt: > 100 C (Miniflash) Seivantennelighet: Ikke relevant Eksplosjonsgrenser, nedre: Ikke relevant Eksplosjonsgrenser, p-vre: Ikke relevant Damptrykk: 16 mmKg (Xalkulert v/20 C) Loselighet i vann: Blar.dbar Spesifikk vekt: 1.10 (vann=l) Damptetthet: r 1 . 0 (luft-1) (Xalkulert v/20 C) Flyktige organiske forbindelser (VOC): 359 g/1 SCAQMD (g/1 av produktet) Fordampningshastighet: < 1 (butylacetatl) Viskositet: 18 centipoise 002152 L9a9b3.1re2J-3.no.: ra g e v vj vi FC-201A? LIGHT WATER (TM) It AFFF (FREEZE PROTECTED) 10 STA3ILITET OG REAXTIVITET. Forhold som skal ur.ngis : Materialer som skal unngis: Ikke kjent. Farlige spaltningsprodukter: Karbonmonoksid og karbondioksid. Hydrogenfluorid. Giftige damper, gasser eller partikler. Tennisk dekomponering av bruksferdig blanding forvenues ikke reprsentera noen risiko. Stabilitet og reakrivitet: S-abilr. Farlig polymerises jor. vii ikke forekomme. 11 OPPLYSNINGER OM HELSEFARS. Effekter ayne: Moderat ayeirritasjon: symptomer kar. vare radhet, hevelse, smerter, trer og uklart syn. Effekter hud: Moderat hudirritasjon (etter langvarig eller gjentatt kontakt): symptomer kan vare radhet, hevelse, klae og tarrhet.. Langvarig eller gjentatt eksponering kan fere til: Allergisk hudreaksjon: symptomer kan vare radhet, hevelse, blemmer og klae. Effekter innnding: En enkelt overeksponering som overstiger anbefalte administrative normer, kan fare tils Plvirkning av sentralnervesystemet : symptomer kan vare hodepine, arhet, savnighet, manglende koordinasjonsevne, nedsatt reaksjonsevne, slaret tale, svimmelhet og i ekstreme tilfeller bevisstlashet. Irritasjon (avre luftveier): symptomer kan vare srhet i nese og svelg, hosting og nysing. Effekter svelging: Dyreforsak utfart med organiske fluorkjemikalier, som finns i dette produktet, viser effekter som leverforstyrrelser, vekttap, manglende appetitt, slavhet samt effekter som pivirker neurologi, bukspytt, binyre og hematologi. Det foreligger ingen kjente helseeffekter for mennesker fra eksponering overfor organiske fluorkjemikalier nir de brukes som forutsatt og i henhold til 00215 L9a9b3.1r2e-f3.no.: Page 27 of 31 FC-201AF LIGHT WATER (TM) It AFFF (FREEZE PROTECTED) veilednir.g. Svelging kan fore til: Pavirkning av nyrer.e: symptomer kar. vare redusert urinmengde, blod i crinen og ryggsmerter. Aspirasjonspneumoni (Lungebetenneise som skyldes at fremmedlegemer kommer ned i lungene. En alminnelig rsak til tilstanden er oppkast.): symptomer kan vare hosting, pustevansker, gisping, hoste opp blod og lungebetenneise som kan vare livstruende. Informasjon om allergi, overfalsomhet: Krefrfremkallende egenskaper: II Arvestoffskadelige egenskaper: Reproduksjonsskadelige egenskaper: -- Andre skadevirkninger, helse: Dette produktet inneholder en eller flere organiske fluorkjemikalier, som muligens kan absorberes og lagres i kroppen over lengre tid, enter, som utgangsmolekylet eller som nedbrytningsstoffer (metabolicter), og kan akkumuleres ved gjentatte eksponeringer. Det foreligger ingen kjente helseeffekter for mennesker fra eksponering overfor organiske fluorkjemikalier nr de brakes som forutsatt og i her.hold til veiledning. Forekomsten av organiske fluorkjemikalier i blodet tilden generelle befolkningen og underpopuiasjonersom arbeidere, har vart publisert siden 1970 rene. 3Ms epidemiologiske studier av egne arbeidere viser ingen skadelige effekter. j St j j i j j j j ii 12 OPPLYSNINGER OM MILJ0FARZ. Persistens/Biologisk nedbrytbarhet: Dette produktet inneholder en eller flere organiske fluorkjemikalier som muligens ikke er nedbrytbare og forblir i miljeet (persistente). Andre skadevirkninger, milje: Nedbrytning: COD (Chemical Oxygen Demand) 1 710 000 mg/kg; BOD 20 dagers (Biochemical Oxygen Demand) ** 494 000 mg/kg; BOD20/COD - 29t. Akvatisk giftighet; 99312-3 FC-201AF LIGHT WATER (TM) 1 \ AFFF (FREEZE PROTECTED) 95 timers LC50 for fisk (Fathead minnow (Pimephales promelas)) 310 mg/1; 13 timers EC50 for daphnia (Water flea (Daphnia magna) ) - > 1000 mg/1; 95 timers IC50 for alge * > 1000 mg/1; 30 minutters EC50, Microtox (Photobacterium phosphoreum) * 63 mg/1; 3 timers EC50, Activated Sludge Respiration Inhibition > 1000 mg/1. Serg for avfallshar.dtering iht. gjeldende forskrifter. 13 FJERNING AV KJEMIKALIEAVFALL. Metoder for fjerning av kjemikalieavfall: Spesialavfall. Lever avfall til en offentlig godkjent innsamler, mottaks- eller behandiingsanlegg for spesialavfall. Anbefalte avfailsgrupper: KOVEDKATEGORI 07: AVFALL FRA ORGANISKE PROSESSER. UNDERXATEGORI 07 05: Avfall fra PBDB av fettstoffer, rengjaringsmidler, desinfeksjonsmidler og kosmetikk. EAX-koce 07 06 03: Halogenerte organiske lasemidler, og moderlut. Avfallstoffnummer: 7151 Organisk avfall med halogen. sipe, vaskevasker Spesieile komponenter: fluorforbindelser. 14 OPPLYSNINGER OM TRANSPORT. ADR-klasse: siffer: bokstav: margnummer: CEFIC-kort: UN-nummer: ICAO-klasse: IMDG-klasse: L99a3b1.r2e-f3.no.: Page 29 o f31 FC-201AF LIGHT WATER (TM) 1% AFFF (FREEZE PROTECTED) IMDG-side: EMS-rvr.: Mr AG: Ovrige opplysninger: Ikke transporzfarlig gods. 15 OPPLYSNINGER OM LOVER OG FORSXRIFTSR. Merkeetikett: S y m b o l (e r ) : KELSSSXADELIG (Xn) Inneholder: 2-(2-butoksyetoksy)-ezar.ol 19 % Etyler.glykol 14 % R-setninger: Farlig ved svelging. Irriterer aynene. . S-setninger: Unr.gi inninding av damp/sproytetike. Unngi konzakt med ayr.ene og langvarig kontakt med huden. Fir man stoffet i oynene; skyll straks grundig med szore mengder vann og kontakt lege. Etter hudkontakt, vask .med sipe og vann. Ved svelging, kontakt lege omgiende og vis der.r.e beholderen eller etiketten. YL-gruppe: 00 -- I I Xorr. yrkeshvg. l.uftbehov: 0-30 m3/l Ovrige opplysninger om merkeetiketten: Ovennevnte merking er basert pi tidligere merkeforskrifter og vil vsre i finne pi produktet en tid fremover. Merkingen vil imidlertid erstattes av folgende merking, som er basert p reviderte merkeforskrifter av januar 1998. Symbol: IRRITER2NDE (Xi) Inneholder: 2-(2-butoksyetoksy)-etanol, 1,2-etandiol Irriterer eynene. Ur.r.gi inninding av damp/sproytetike. Unngi kontakz med oynene. Unngi langvarig eller gjentatt kontakt med . huden. Fir man stoffet i oynene; skyll straks grundig med stre mengder vann og konzakt lege. Etter hudkontakt, vask med sipe og vann. Ved svelging, drikk to glass vann og kontakt lege umiddelbart. 0 j9L9u3u1.r2u-j3.nv.; u g c 'y y j 1 FC-201AF LIGHT WATER (TM) U AFFF (FREEZE PROTECTED) YL-gruppe: 00 Spesielle lover og bestemnelser: 1. Forskrift om utarbeidelse og distribusjon av Heise-, Miljo- og Sikkerhetsdatablad for farlige kjemikalier. 2. Forskrift ora klassifisering og merking av farlige kjemikalier. 3. Forskrift om klassifisering, merking ra.v. av stoff som kan mediare miljafare. 4. Forskrift om stoffkartotek. 5. Forskrift om spesialavfali. 6. Etc. 1 16 ANDRE OPP1YSNNGER AV 3ETYDNING FOR BRUKERNSS SIXXERH2T OG HELSE. Anvendelsesomrider: Filmdannende skumkor.sentrat for slukking av brann i hydrokarboner. Viktigst kilde(r) ved utarbeidelsen av HMS-databladet: Datablad fra 3M General Offices, St. Paul, Minnesota, USA. i i Arsak til oppdatering: Hele databladet er revidert. Tilpasset nye merke- og HMS-databladforskrifter, fastsatt i januar 1998. Etc. Egne opplysninger: Informasjonen p dette HMS-databladet er i henhold til vire nvarence opplysninger og beste oppfatning angende riktig bruk og hndteringen av dette produktet under normale forhold. Enhver bruk av produktet som ikke er i samsvar med dette HMS-databladet, eller som medforer bruk av produktet i kombinasjon med andre produkter eller enhver annen prosess er brukerens ansvar. i i i I SI I -f I I !i i I 99312-3irfuc/./ cy./(C/.. rage n oj it FC-201AF LIGHT WATER (TM) It AFFF (FREEZE PROTECTED) FOLGENDE PRODUXTER DEXXZS AV DETTE HMS-DATA3LADET: 3M ID-nr : ZF-0002-0842-9 3M Light Water 1% AFFF Fire Fighting Foam, FC-201AF, 200 L 3M ID-nr : ZF-0002-0341-1 3M Light Water 1% AFFF Fire Fighting Foam, FC-201AF,1000 L 3M ID-nr : XF-0008-020S-8 3M Light Water 1% AFFF Fire Fighting Foam, FC-201AF, Bulk 3M ID-nr : XF-0008-0203-3 3M Light Water 1% AFFF Fire Fighting Foam, FC-201AF,1000 L 3M ID-nr : XF-0008-0202-5 3M Light Water 1% AFFF Fire Fighting Foam, FC-201AF, 200 L 3M ID-nr : XF-0008-0200-9 3M Light Water 1% AFFF Fire Fighting Foam, FC-201AF, 20 L 2158 BEST COPY AVAILAB RF- Rogaland Research RO.Box 2503 Ullandhaug 4004 Stavanger Norway Telephone: +47 51 87 50 00 Telefax: +47 51 87 52 00 Visiting address: Professor Olav Hanssensvei 15 Bergen: Thormohlensgt. 55 5008 Bergen Norway Telephone: +47 55 54 38 50 Telefax: +47 55 54 38 60 Internet: http : / / www.rf.no