Document qd8KxRzO9GRoaB1ydBM1G0rMR

Wildlife International, Ltd.__________________________ Project Number 454A-103A - 1- PFOS: A 96-HOUR TOXICITY TEST WITH THE FRESHWATER ALGA (Selenastrum capricornutum) ADDENDUM WILDLIFE INTERNATIONAL, LTD. PROJECT NUMBER: 454A-I03A 3M LAB REQUEST NO. U2723 U.S. Environmental Protection Agency Series 850 - Ecological Effects Test Guidelines OPPTS Number 850.5400 and OECD Guideline 201 This study was reported using a test substance purity of 90.49%. The test substance characterization was revised on 9/7/00 and the purity was determined to be 86.9%. The Sponsor requested that the results be revised based on the most recent purity analyses. The results previously presented were simply corrected by the ratio of the two purities or a factor of 0.9603. No attempt was made to recalculate analytical standard concentrations or measured concentrations from original raw data. As such, minor errors due to rounding may be present. Attached is the revised summary page from the final report and the most recent certificate o f analysis. Nj om j fV] Hi HO Wildlife International. Ltd. 2- - Project Number 454A-103A REVISED STUDY SUMMARY BASED ON A PURITY OF 86.9% SPONSOR: SPONSOR'S REPRESENTAUVE: LOCATION OF STUDY, RAW DATA AND A COPY OF THE FINAL REPORT: 3M Corporation Ms. Susan A. Beach Wildlife International Ltd. Easton, MD 21601 WILDLIFE INTERNATIONAL LTD. PROJECT NUMBER: TEST SUBSTANCE: STUDY: MEAN MEASURED TEST CONCENTRATIONS: TEST DATES: LENGTH OF EXPOSURE: TEST ORGANISM: SOURCE OF TEST ORGANISMS: 454A-103A PFOS (Perfluoroocatane Sulfonic Acid Potassium Salt) PFOS: A 96-Hour Toxicity Test with the Freshwater Alga {Selenastrum capricomutum) Negative Control, 5.3, 11, 20, 42, 83 and 172 mg a.i./L Experimental Start - April 1-2, 1999 Exposure Termination - April 16,1999 Experimental Termination -April 21, 1999 96Hours Freshwater Alga {Selenastrum capricomutum) Wildlife International Ltd. Easton, Maryland 21601 CELL DENSITY 72-HOUR EC50: 95% CONFIDENCE LIMITS: 96-HOUR EC10: 95% CONFIDENCE LIMITS: 96-HOUR EC50: 95% CONFIDENCE LIMITS: 96-HOUR EC90: 95% CONFIDENCE LIMITS: 72-HOUR NOAEC: 96-HOUR NOAEC: 67 mg a.i./L 42 and 75 mg a.i./L 47 mg a.i./L 41 and 48 mg a.i./L 68 mg a.i./L 63 and 70 mg a.i./L 132 mg a.i./L 101 and 147 mg a.i./L 42 mg a.i./L 42 mg a.i./L Wildlife International, Ltd. REVISED SUMMARY (Continued) AREA UNDER THE GROWTH CURVE 72-HOUR EC50: 95% CONFIDENCE LIMITS: 96-HOUR EC 10: 95% CONFIDENCE LIMITS: 96-HOUR EC50: 95% CONFIDENCE LIMITS: 96-HOUR EC90: 95% CONFIDENCE LIMITS: 72-HOUR NOAEC: 96-HOUR NOAEC: 71mga.i./L 53 and 79 mg a.i./L 47 mg a.i./L 38 and 48 mg a.i./L 68 mg a.i./L 64 and 71 mg a.i./L 139 mg a.i./L 120 and 149 mg a.i./L 42 mg a.i./L 42 mg a.i./L GROWTH RATE 72-HOUR EC50: 95% CONFIDENCE LIMITS: 96-HOUR EC 10: 95% CONFIDENCE LIMITS: 96-HOUR EC50: 95% CONFIDENCE LIMITS: 96-HOUR EC90: 95% CONFIDENCE LIMITS: 72-HOUR NOAEC: 96-HOUR NOAEC: 115 m ga.i./L 99 and 127 mg a.i./L 57 mg a,i./L 52 and 60 mg a.i./L 121 mg a.i./L 110 and 133 mg a.i./L >172 m g a.i./L N ot Calculable 42 mg a.i./L 42 mg a.i./L Project Number 434A-1Q3A Wildlife International. Ltd. -4 - CERTIFICATE OF ANALYSIS Project Number 454A-103A INTERIM CERTIFICATE OF ANALYSIS Revision 1(9/7/00) Centre Analytical Laboratories COA Reference #: 023-018A 3M Product: PFOS, Lot 217 Reference #: SD-018 Purity: 86.9%_____________ Test Name Result Inorganic Anions (IC) 1. Chloride 2. Fluoride 3. Bromide 4. Nitrate 5. Nitrite 6. Phosphate 7. Sulfate4 Organic A cids5(IC) 1. TFA 2. PFPA 3. HFBA 4. NFPA Elemental Analysis': 1. Carbon 2. Hydrogen 3. Nitrogen 4. Sulfur 5. Fluorine ::: if:i: y:=:::::: ..:.i..... ........ ='=^:..::.U.:...;:: ; s - S i ' u H i i Sxi:i i s e s; sis;;::S;s i- :: :;:i; ;;ij; :; . :T:f?:fKTTT?--" !:=: V?=-- -T":"=T::^T;-"] ... - - - - - : : ^ i ir:i ::: 5::: ::1;::ii::r!! :. ... SiSlsS-siSili : ;-?::- j; H ??=iStiFilir ?iSi='E g-x :iE:fS-:rf:'!':->: . ... 1. Theoretical Value = 17.8% 2. Theoretical Value = 0% 3. Theoretical Value = 0% 4. Theoretical Value = 5.95% 5. Theoretical Value = 60% <0.015 wt./\vt.% 0.59 wt./wt.% <0.040 wt./wt.% <0.009 wt.Avt.% <0.006 wt./wt.% <0.007 wt./wt.% 8.76 wt./wt.% 1. <0.1 wt./wt.% 2. <0.1 wt./wt.% 3. 0.10wt./wt.% 4. 0.28 wt./vvt.% 1. 12.48 wt./wt.% 2. 0.244 wt./wt.% 3. 1.74 wt./wt.% 4. 8.84 wt./wt.% 5. 54.1 vvt./wt.% COA023-018A Page 1 o f 3 INTERIM CERTIFICATE OFANALYSIS Centre Analytical Laboratories COA Reference #: 023-018A Date o f Last Analysis: 08/31/00 Expiration Date: 08/31/01 Storage Conditions: Frozen -10C Re-assessment Date: 08/31/01 1Purity = 100% - (sum o f metal impurities, 1.45% +LC/MS impurities, 8.41%+Inorganic Fluoride, 0.59%+NMR impurities, 1.93%+organic acid impurities, 0.38%+POAA, 0.33%) Total impurity from all tests = 13.09% Purity = 100% - 13.09% = 86.9% 2Potassium is expected in this salt form and is therefore not considered an impurity. 3Purity by DSC is generally not applicable to materials o f low purity. N o endotherm was observed for this sample. 4Sulfur in the sample appears to be converted to SO4 and hence detected using the inorganic anion method conditions. The anion result agrees well with the sulfur determination in the elemental analysis, lending confidence to this interpretation. Based on the results, the SO4 is not considered an impurity. 5T F A HFBA NFPA PFPA Trifluoroacetic acid Heptafluorobutyric acid Nonofluoropentanoic acid Pentafluoropropanoic acid 6Theoretical value calculations based on the empirical formula, CsFnSCVK* (MW=538) This w ork was conducted under EPA G ood Laboratory Practice Standards (40 CFR 160). COA023-018A Page 2 of 3 INTERIM CERTIFICATE OFANALYSIS Centre Analytical Laboratories COA Reference #: 023-018A LC/MS Purity Profile: Im purity C4 C5 C6 C7 Total wt./wt. % 1.22 ' 1.33 4.72 1.14 8.41 Note: The C4 and C6 values were calculated using the C4 and C6 standard calibration curves, respectively. The C5 value was calculated using the average response factors from the C4 and C6 standard curves. Likewise, the C7 value was calculated using the average response factors from the C6 and C8 standard curves. Prepared By: ________________________________ ____ David S. Bell Date Scientist, Centre Analytical Laboratories Reviewed By: ________________________________ ____ John Flaherty Date Laboratory Manager, Centre Analytical Laboratories COA023-018A Page 3 of 3 ildlife International, Ltd. ECOTOXICOLOGY & ANALYTICAL TESTING SERVICES September 27,2000 Ms. Susan A. Beach 3M Corporation Environmental Laboratory 935 Bush Avenue 3M Building 2-3E-09 P.O.Box 33331 St. Paul, Minnesota 55133 Dear Susan: Enclosed please find Addendums for Wildlife International, Ltd. Project Numbers 454A-101, 454A-102,454A-103A, 54A-104,454A-105,454A-106,454A-107,454A-108 and 454A-109 If you have any questions, please let me know. KRD/cyw8/3m Enclosures Senior Biologist 8598 Com m erce Drive Easton. Maryland 21601 -Tel.410-822-8600 Fax 4 10-822-0632 E-mail ecotox@wildlifeintemational.com European Office: Overscheenseweg 11A 1412 AA Naarden-The Netherlands-Tel. 31(0)356949261 Fax 3 1(0)356948229 E-mail aleopold@worldonline.nl