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ACUTE TO XICITY TO AQUATIC INVERTEBRATES (E.G..DAPHNIA)
TEST SUBSTANCE___________________________________________ __
Identity: Perfluorooctanesulfonate, Lithium salt; may also be referred to as PFOS Li salt, FC-94, or FC-94-X. (1-Octanesulfonic acid,
CAS # 29457-72-5)
Rem arks: The test sample is a mixture of the test substance in water (approximately 24.5% test substance and 75.5% water). No calculations were made to adjust for the actual concentration of the test substance in the test sample-.
METHOD________________________ ______________________________
Method: Not noted. Test type: Static acute GLP: No Y ear Com pleted: 1994 Species: Daphnia magna A nalytical m onitoring: pH and DO content Statistical m ethods: EC50 values calculated using Trimmed Spearman-
Karber method Test daphnid source: Obtained from U.S. EPA-NETAC, Duluth, Minnesota. Test daphnid age at study initiation: < 24-hours
Test conditions Dilution water: Carbon-filtered well water Dilution w ater chem istry: 8.4 8.6 mg/L Stock and test solutions preparation: A primary stock solution was prepared in dilution water to yield a test sample concentration of 1000 mg/L. All test solutions were made by diluting the appropriate amount of stock solution with dilution water to make 50 mL of solution per concentration. Exposure vessels: 100 mL glass beakers containing 50 mL of test solution. Num ber o f replicates: 4 Num ber o f daphnids per replicate: 5 Num ber o f concentrations: five plus a negative control W ater chem istry during the study: Dissolved oxygen at test term ination: 7.0 mg/L (control exposure)
001535
7.8 mg/L (1000 mg/L exposure) pH at test term ination:
8.6 (control exposure) 8.6 (1000 mg/L exposure) Test tem perature range ( 0 - 4 8 hours) 20.1-21.0C Elem ent basis: mortality and immobilization
RESULTS
Nom inal concentrations: Bk control, 100,180, 320, 56 0,1 0 0 0 mg/L Elem ent value: 24-hour EC50 = 330 (290-370) mg/L
48-hour EC50 = 210 (190-230) mg/L v 48-hour NOEC = 100 mg/L S tatistical Evaluation: The EC50 values and 95% confidence intervals were calculated using the Trimmed Spearman-Karber method with trim set to 0%. M ortality o f controls: None Rem arks: Values reported are for the test sample. No calculations were made to adjust for the concentration of the test substance in the test sample.
CONCLUSIONS_____________________________________________ .
The test sample containing 24.5% Perfluorooctanesulfonate, Lithium salt exhibited a 48-hour EC50for Daphnia magna of 210 mg/L.
Subm itter: 3M Company, Environmental Laboratory, P .O : Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY ___________________________
R eliability: Klimisch ranking 2. This study, while well conducted, lacks analytical data for: determination of the test substance concentration in the test solutions; and determination of the sample purity.
REFERENCES_____________________________
This study was conducted by the 3M Company, Environmental Laboratory, Lab Request number M 1 018 ,2/10/94.
O T H E R ________________________________________________
Last changed: 5/2/00
001536
ENVIRONMENTAL LABORATORY 48-HR ACUTE TOXICITY TO DAPHNIA, DAPHNIA MAGNA
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TYPE TEST: -
STATIC X ___ STATIC RENEWAL_________
TEST SUBSTANCE:
DILUTION WATER:
CARBON-FILTERED WELL WATER (3M WELL #2. ST. PAUL. MN1______________ TEMP. <* *C, pH &.H O.O. *.<b mg/L
TEST DAPHNID SOURCE: U.S.EPA-NETAC. Duluth. Mn___________ DATE RECEIVED: Co
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DAPHNIDAGE: <24 HR OLD NEONATES
NO. OF DAPHNIDS/TEST CHAMBER:
5
LOADING RATE: DATE INITIATED:
TEST SOLUTION VOLUME: ft ^\
50 mL SIZE OF TEST VESSELS: DATE TERMINATED:
lOOmL . 5-
TEST" INITIAL TOTAL
VESSEL CONC. AMOUNT mg/L ADDED*
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ENVIRONMENTAL LABORATORY
LR: Date: Analyst:
M1018 2 /8 /9 4 JBD
Daphnia magna 48-hour Toxicity Test Data Summary
SAMPLE: FC-94-X
Sample Cone. No. Organisms
(m g/L)
Exposed
100 %
20
180 20
320 20
560 20
1000
20
% Immo xlized @
24 hours
48 hours
00
0 25
45 100
100 100
100 . 100
24 h EC50 (95% C.l.) = 48 h EC50 (95% C.I.) =
330 mg/L (290-370) 210 mg/L (190-230)
EC50 Estimation Method: Trimmed Spearman-Karber 24 h: 0% trim 48 h: 0% trim
001538
.ISLINGTON RESEARCH. INC. TRIMMED SPEARMAN-KAE3ER METHOD FOR CALCULATION OF
EC50 AND LC50 VALUES IN BIOASSAYS
W V Q
FOR REFERENCE. CITE M.A. HAMILTON. R,,C. RUSSO. AND R.V. THURSTON. 1977. TRIMMED''S P E A R M A N - K A R B E R M E T H O D 'F OR ESTIM A T I N G 'M E DIAN LETHAL CONCENTRATIONS IN TOXICITY BIOASSAYS. ENVIRON. SCI. TECHNQL. 11(7) 714-719 CORRECTION 12(4) 417 (1973).
DATE TEST *
2/S/94 M101S
BEST COPY AVAILABLE
CHEMICAL
FC-94-X
SPECIES
D. ma a n a
DURATION
24 hours
RAW DATA
C O N C E N T R A T I O N (m q / L ) 1 0 0 . 0 0 130.00 320.00 5 6 0 . 0 0 1 0 0 0 .0 0
NUMBER EXPOSED
2 0 20 20 20 -20
MORTALITIES
0 0 9 20 20
rs 9 o
SPEARMAN-KARBER TRIM
SPEARMAN-KARBER ESTIMATES
eC50
327
957. L O W E R C O N F I D E N C E
289.02
957. U P P E R C O N F I D E N C E
372.05
001539
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m COPY AVAILABLE
BURLINGTON RESEARCH. INC,
6 .907E .
Trimmed SDearman-Karber Method for Calculation of EC50 and LC50 Values in Bioassavs
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LC10
LC25
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LC100
MORTALITIES
001540
iirtTE
2/8/94
TEST #
M101S
CHEMICAL
FC-94-X
SPECIES
D. maana
DURATION
.48^hours'>
RAW DATA
C O N C E N T R A T I O N (m a / L ) 1 0 0 . 0 0 130.00 3 2 0 . 0 0 5 6 0 . 0 0 1000.00
M IM
NUMBER EXPOSED
20
20 .
20
20
MORTALITIES
0 5 20 20
SPEARMAN-KARBER TRIM
0.00
SPEARMAN-KAR3ER ESTIMATES
eC50
207 .5236053
957. L O W E R C O N F I D E N C E .
1S5.42
957. U P P E R C O N F I D E N C E
232.26
BEST COPY AVAILABLE
001541
