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ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity:[2-(N-Ethylperfluorooctanesulfonamiedtoh)ylacrylate;may also be referredtoas B1228, D-1, ETFOSEA, or FX-13.(2-Propenoicacid,2[ethyl[heptadecafluorooctyl)sulfonyl]aminoe]settehry,ClAS # 423-82- 5) Remarks:MateriiaslanambersolidS.amplepuritaynd notrecorded3.M lot number 13. METHOD Method: Not given Type: Staticacute range-finder GLP: No Year completed: 1984 Species: Pimphalespromelas Supplier:Brady,Neb. Analyticalmonitoring:Nominal concentrationosnly. Temperature,pH and dissolvedoxygen recorded. Exposure period:96-hours Statisticamlethods: LC5o could not be calculatedas therewas insufficient toxicitaytconcentrationsabove thewater solubility. Test fishage: Not given Length and weight:: Length: 3.79 cm Weight: 0.359 g Loading: 0.6 g/L Test conditions: Dilutionwater: Carbon-filterewdellwater Dilutionwater chemistry during the study: DO: 6.5 - 9.3 mg/L pH: 7.9-8.2 Test temp. range: 21-220C Stock and testsolutionpreparation:Test solutionsprepared by mass additionoftestsubstance to3L of dilutiownater. Concentrations dosing rate:Once Stabilityof the testchemical solutions:Not recorded Exposure vessels:Wide mouth one gallonglassjars Exposure volume: 3 liters Number of replicates:one Number of fishper replicatef:ive Number of concentrations:Four plusa negativecontrol RESULTS Nominal loadingconcentrations:Bk control1,0, 100, 500, 1000 mg/L Element value: 96-hour LC5o > 1000 mg/L PercentMo lity NominalTest Concentration,24-Hours 48-Hours mg/L I NegafivCeontrol 0 0 10 0 0 100 0 0 500 0 0 1000 0 20 72-Hours 0 0 0 0 20 96-Flours 0 0 0 0 20 Remarks: Allvalues reportedare fornominalconcentrations.No measurement oftestconcentrationswas conducted. CONCLUSIONS The Et FOSEA 96-hour LCBOforfatheadminnow was determined to be > 1000 mg/L DATA QUALITY ReliabilityW:imisch ranking2.This study,whileconducted usingthe methodology acceptableatthe time,lacksanalyticadlata fordeterminationof the testsubstance concentrationinthetestsolutionsand determinationofthe sample purity.Testingwas alsodone wellabove the solubilitlyimiotf the test substance. REFERENCES This study was conducted by the 3M Company, EnvironmentalLaboratory,Lab Request number B1228, 4/l/84 OTHER Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul, Minnesota,55133 Last changed: 5/22/00 ENVIRONMENTAL CHEMICAL ENVIRONMENTAL TOXICITY TEST: LABORATORY EFFECTS EVALUATION SUMMARY REPORT Chemical Tested )_Ap7-/_5 Test Typ EC50/ICSO/GRSO (95% C.L.):a(&(z Test Species: Aqhc#--A-41"AJA,10a) 14zoone izw& zaw Test Date::5-.2-9-pv Analyst(s): A..r - qczr--14 Exposure Type: L.R. (E:4sure/Test Conditions) mg/l Static_ Static.RecirculatoryDiluent: giatic/Continuous Orbital Shaking Static Renewal Flow-Through Aerated Well'.!t4ate(rInitial D.O._t_5mg/ly . Water Hardness: -A-7-WI) pm. Hardnes Clasification: Dissolved Oxygen t Saturation: pH_OZ.Z; Temp. ;Z.-/ec(mop)) Control(s) Lowest Experimental Dose Highest Experimental Doselooo pH Levels: 48-hr 96-hr 7 Extended Enosure Control(s) Lowest Experimental Dose 10045tf Highest Experimental Dose 1000 *@SIP Test Temperature OC (OF): 48-hr 96-hr Extended Exposure Photoperiod: 48-hr. 96-hr Extended Exposure ?-IOC(16) ZZOC(IZ) oc( Sixte_enhours light with a 15-30 minute transition period. ENVIRONMENTAL IABORATORY AOUATIC TOXICITY DATA SHEET EXPLORATORY (RANGE-.FINDING TEST) Type Tes Material Tested Test Organismjtm-=-i4/A49W-)so a Avg. Wt. _40,ffL:V &raw Avg. Size,3!ECM Date Recld No. Organisms per.dosre-7- LO Batch N o 5 2-rL-H Test Solution Volume 3 Liters/All-GlassTank Tank Dimensions:WIDE-MOUTH I GALLON GLASS OR Time Started_ZZZ4:@@ Date Started Date Complete d Dilution Water Carbon-filtered-wellwater (Temp.-Zll D. 0. ppm, pH_e,2j Involitiga (FullSign Init Total -tk Conc: Amount MALI- Added 2N-4 urs Total D.0 No.' Dead PH IDom so (9S% Confidence Limits) 48 Hours 7Z HLoHurPs.0, Totald H ID.O. Total No.De om NO.Dead I vom o9t6alHo 0. @NoN.Dead- N Lin Contrvoi IL-) -1 -.aso inn LOI. Test Temp. oc. Data Effects other than Mortality Tank No. Symptoms or Reactions Control. 24-Hr. ./ EC so Total No. Affected 48 Hr. 72-Mr. /I //I 96-Mr. CtvmmLnts: