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ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA)
TEST SUBSTANCE______________________________________________ __
Identity: A mixture containing perfluorooctanesulfonate; may also be referred to as PFOS, FC-95, or as a component of FC-206CF or FC-206CF-X. (1-Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was 101. The test sample is FC206CF. Current information indicates it is a mixture of 0.5% PFOS, 10% diethylene glycol monobutyl ether, 78.08% water, 1.375% acrylic foamer, 1.5% sodium octyl sulfate, 1% sulfonated alkyl ether salts, 6.0% urea, 0.5% triethanolamine, and 0.05% tolyltriazole.
The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect the toxicity of the fluorochemical component of the test sample.
METHOD:_________________________________________________________
Method: OECD Guideline 202 Type: Acute static GLP: No Year completed: 1991 Species: Daphnia magna Supplier: In-house cultures maintained at EnviroSystems Division, Resource Analysts, Inc., Hampton, NH. Analytical monitoring: Temperature, pH, conductivity, and dissolved oxygen. Exposure period: 48-hours Test organism age: <24 hours old Statistical methods: No calculations performed, based on study results. Test conditions:
Dilution water: Filtered natural well water Dilution water chemistry:
Conductivity: 840 pmhos/cm pH: 7.6
Lighting: Cool-white fluorescent lights at 20 pEin/sec/m2. A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. Stock and test solution preparation: A primary 1000 mg/L stock solution was prepared and added directly to create test solutions. Exposure vessels: 250 mL beakers containing 200 mL exposure solution at a water depth of approximately 6 cm. Number of replicates: 4 Number of daphnids per replicate: 5
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Number of concentrations: five plus a blank control Water chemistry during the study:
pH range (0-48 hours): 7.6 - 8.3 (control exposure) 7.7 - 8.3 (1000 mg/L exposure)
Temperature range (0-48 hours): 19.0 - 20.2 C (control exposure) 19.1 - 20.3 C (1000 mg/L exposure)
Conductivity range (0-48 hours): 840 - 880 pmhos/cm (control exposure) 850 - 880 pmhos/cm (1000 mg/L exposure)
Dissolved oxygen range (0-48 hours): 7.6 - 8.3 mg/L (control exposure) 7.7 - 8.3 mg/L (1000 mg/L exposure)
RESULTS____________________________________________________
Nominal concentrations: Blank control, 150, 250, 400, 600, and 1000 mg/L.
Element values: 48-hour EC50 = >1000 mg/L
Element values based on nominal concentrations.
Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
CONCLUSIONS___________________________________________________
The test substance 48-hour EC50 was determined to be >1000 mg/L. No mortality or sublethal effects were observed.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY___________________________________________________
Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution.
REFERENCES____________________________ ________________________
This test was conducted by EnviroSystems Division, Resource Analysts, Incorporated, of Hampton, NH at the request of the 3M Company, St. Paul, MN, Lab Request number J1884-4, 1991.
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i. s u h h a r y
The acute toxicity of FC-206CF to the daphnid, Daphaia magaa, is described in this final report. The test was conducted for 3N Conpany for 48 hours during June 10 to 12, 1991, at the EnviroSysteas Division of Resource Analysts, Inc. in Haapton, Hew Haapshire. It was conducted by Jeanne Magazu, Peter Kowalski, Ellen Stanford, Robert Boeri, and Tiaothy Ward.
The test was performed under static conditions with five concentrations of test substance and a dilution water control at a temperature of 20 1C. The dilution water was filtered natural well water collected at Haapton, New Haapshire. Aeration was not eaployed to aaintain dissolved oxygen concentrations above an acceptable level. Noainal concentrations of FC-206CF were: 0 ag/I> (control), 150 ag/L, 250 ag/L, 400 ag/L, 600 ag/L, and 1,000 ag/L. Noainal concentrations were used for all calculations.
Daphnids used in the test were produced froa an in-house culture and were less than 24 hours old at the start of the test. After 48 hours of exposure the control daphnids had an average wet weight (blotted dry) of 0.0006 g, resulting in a loading rate of 0.015 g/L. All daphnids were in good condition at the beginning of the study.
The test substance was not toxic to daphnids. Exposure of daphnids to the test substance resulted in a 48 hour EC50 greater than 1,000 ag/L FC-206CF.
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SECTIOW:
f. , II.
Sunaary Table bf Contents
III. Index of1Tab' les'' :>*'
IV. Introduction
V. Methods and Materials
VI. Results
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III. TOSE or TABLES AED flCOTES
Table 1.
Cheeical characterization of a representative
sanple of natural well water used as dilution water for toxicity test ,.
'Table 2. Survival data fro toxicity test
'-- Table 3. '-A
Hedan effective concentrations (EC50s) fron toxicity test -
Table A.l. Conductivity, pH, temperature, and dissolved
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This study was sponsored by 3M Company, St. Paul, Minnesota. The objective of the study was to determine theacute toxicity of TC-206CF to
the dapbnid, a freshwater invertebrate. ifThe report contains sections that
describe the methods and materials employed in the study, and the results
of the `investigation. The report also contains an appendix that presents 'the water quality data collected during the test.
A.V rt TEST SOBSTANCE:
METHODS JUR) MATEXXUS
T' - i FC-206CP;(EnviroSystems Sample Humber 2938E) was delivered to
EnviroSystems< on May 23, 1991. It was contained in a 4 ox. plastic bottle
that was .-labelled with the following information: "J1884-4, FC206CP".
FC-206CF (a .light brown liquid) was supplied by 3M Company, 935 Bush
iAvenue, St; .Paul/Minnesota. Prior to use the test material was stored in
the dark at room temperature. A reserve sample (approximately 1.0 grams)
will be.retained.-at EnviroSystems for a minimum of 10 years.
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" DILOTION WATER: i'- .
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ior acclimation of test organisms and for all toxicity
testing-;:,',was?; collected from wells at EnviroSystems in Hampton, New
Hampshiretv^iHater'^was stored in 500-gallon polyethylene tanks where it was
aerated;ii/t,ie R. eV' ults^of chemic___a^l analysi.s o. cf ^a represe___n_t__a__t__i__v__e_____s__a__m__p_ "lI .e .oaf uw.ait. _e__r_
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Tabla 1. Cheaical characterii-ion of representativa saaple of natural ell atar usad as dilution atar for toxicity tast
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TOXICITY TBSTIHG:
The definitive toxicity test was performed during June 10 ..to 12, 1991. It is based on procedures of the OXCD (1984). The test was
conducted at a target teaperature of 20 1C with five concentrations
of test substance and a dilution water control. X 1,000 mg/L stock solution was femulated without the use of a solvent. Noainal concentrations of the test aaterial were: 0 ag/L (control), 150 ag/L, 250 ag/L, 400 ag/L, 600 ag/L, and 1,000 ag/L.
Twenty daphnids were randoaly and equally distributed aaong four replicates of each treatment. The test was perforaed in 250 al glass beakers that contained 200 al of test solution (water depth was approximately 6 ca). Test vessels were randoaly arranged in an incubator during the 48 hour test (a randoa numbers table was used to select the location of each vessel). A 16 hour light and 8 hour dark photoperiod was autoaatically aaintained with cool-white fluorescent
lights that provided a light intensity of 20 uEs-1a-x . Aeration was not required to maintain dissolved oxygen concentrations above acceptable levels. Daphnids were not fed during the test.
The nuaber of surviving organises and the occurrence of
iaaobilization (inability to swim within 15 seconds after gentle
agitation) or other sublethal effects (loss of equilibriua, erratic
swiaaing, loss of reflex, excitability, discoloration, or change in
behavior) were determined visually and recorded initially and after 24
and 48 hours. Dissolved oxygen (YSI Model 57 aeter; instrument nuaber
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PRL-3), pH (Beckaan model pHI 12 meter; instrument nuaber PRL-51), conductivity (Cole; Parmer model .nuaber 1481-60, instrument nuaber
PRL-50), -iand teaperature (ASTM mercury thermometer; thermometer nuaber
2265) ;?',were :measured and recorded daily 'in each test chaaber that
contained live animals.. J'.............
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STATISTICAL HKTHODS:
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standard &'statistical techniques,(Stephan ,1983) because 50% survival or - :
, greater, occurred.in ,all:.tested,concentrations.
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M l test vessels containing FC-206CF re initially clear and reaained clear throughout the test. Biological and water quality data generated by the acute toxicity test are presented in Table 2 and Appendix A, respectively. One hundred percent survival occurred in the control exposure. Control daphnids had an average wet weight (blotted dry) of 0.0006 g at the end of the test. Loading rate during the toxicity test was approxixately 0.015 g/L.
The 24 and 48 hour EC50s for daphnids exposed to FC-206CF are presented in Table 3. The 48 hour EC50 was greater than 1,000 ag/L FC-206CF.
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Table 2. Survival data froi toxicity test
Moeinal
Concentration .` (ej/L) - rep.
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T*bl 3. Radian effectiveconcentrations (ECSOs) fron toxicity test
Exposure " '
pariod
EC50
''-95 percent
confidence Units
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V' 'Y-' ..a ; VII. REFERENCES
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OECD. 1981. Decision of the Council Concerning the Hutual Acceptance of Data in the Assessment of Chemicals. Annex 2. OECD Principles of Good Laboratory PracticeC-jC (81) 30 (Pinal).
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'OECD. 1984. Guidelines for Testing of Chemicals. Section 2: Effects on Biotic Systems. ^ M e t h o d 202, Daphnid sp.. Acute Immobilization Test and
^ Reproduction Test.fi Adopted April 4, 1984.
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T M A.l. Conductivity, pH, teape-ature, and diaaolved oxygen concentration aeasured during toxicity teat
Hoainal
Conductivity (ueho/ce)
PH
concentration (eg/U ' Rep.
0 hr
24 48 hr , hr
0 24 hr hr
0 control. 1 840 870 880
7.6 7.7
2 3
840 840
870 870
880 7.6 880 - 7.6
7.7 7.7
4 840 870 880
7.6 7.7
150
1 840 860 900 2 840 860 900 3 840 860 900 4 840 860 900
7.6 7.7 7.6 7.7 7.6 7.7 7.6 7.7
250 1 840 860 880 2 840 860 880 3 840 860 880 4 840 860 880
7.6 7.7 7.6 7.7 7.6 7.7 7.6 7.7
400
. 1 840 860 870
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840 840
860 860
870 870. '
7.6 7.6
7.7 7.7
600
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48 hr
8.3 8.3 8.3 8.3
8.3 8.3 8.3 8.3
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