Document qNBaZV7Ln4qee9dELEq54rYn

Material Tested r----yj E. I. du Font de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine J -- -- n I -HASKELL LABORATi:)RY REF'ORT NO. 737-77 Sample Ready Haskel1 Nu. For Testing Other Codes 11,336 6-23-77 ------J Mat Cht'"'!cals Jacki^'n L PRIMARY SKIN IRRITATION AND SENSITIZATION TESTS ON GUINEA PIGS Procedure: The test for primary irritation was-conducted by applying, and lightly rubbing in, I dro 60% and 6% solution (vol/vol) of the test material 'n 0.9^ saline on the shaved intact shoulder skin guinea pigs. To test for the sensi+izatlon potential, a series' of four sacral in+radermal injection week over a three-week period, which consisted of O.I ml of a \% solution (vol/vol) of test material Following a two-week rest period, the test animals were challenged for sensi+lza+lon by applying, an I drop (=0.05 ml) of a 60% and 6% solution (vol/vol) of test material in 0.951 saline on the shaved i A group of 10 previously unexposed guinea pigs received similar applications at the time of challeng comparison of the challenge reactions on skin of similar age. Results: Concentration in 0.9% saline Primary Irritation Test-24 Hours -48 Hours Cha11enge Test-24 Hours -48 Hours Sensi+lzation Response Ratio** Reactions^ on Intact guinea Pig Skin Test Areas (10 Animals - In. Avg. W+. Final Avg. Wt. 498 g 729 g) Control Areas for (10 New Animals - Av ^ 60? 1+, 9 neg. 10 neg. 10 neg. 10 neg. 10 neg. 10 peg. 10 neg. 10 neg. 0/10 10 neg. 10 neg. . Docs if AR226-2862 -2- ^yiMiBry.: I ^|BIB|^^^^^IB^^^III|HIHIIHlBI^^B(caused slight to no irritation at 24 hrs a In 0.911 SSlTne.N^l^M'a+lor^Ia^obse^e^^^DS^oncen'Tra+lon and no ijcnsltiza+lon observed at ch denna+l+ls hazard Is expected from this material if flood Industrial hygiene (-''"ac+ices are followed. * Reaction: +, ++, +++ = mild, moderate, strong erythema; ++-+ = erythema plus edema; +++++ = ne ** Sensi+lza+ion Is defined as a significant score increase at challenge over the response expecte amount applied initially or on the Iconcu'-rent controls. Composition: TM^^--J JDT:ms Report No. 757-77 Date Issued: September 25, 1977 Report by: Approved by; ^ff^w^A^ /i^-nrL. James D. ffitylor lexicologist i ^ ^ W ^ ^ aymond W. Morrow Chief, Dermal and Ocular Toxi