Document q4qnEgKDw1jyMg2daVRqo1Vq

fiAX.9>G_ 430 TRADE SECRET M A M -& U l DuPont-6818 Study Title H-24616: Static, Acute, 96-Hour Screening Test to Pim ephales prom etas Laboratory Project ED: DuPont-6818 A u th o r : Daniel J. Peterson, B.S. St u d y C o m pleted o n : July 11,2001 Pe r fo r m in g L a bo r a to r y : E.I. du Pont de Nemours and Company Haskell Laboratory for Health and Environmental Sciences Elkton Road, P.O. Box 50 Newark, Delaware 19714-0050 Company Sanitized. Does net contain TSCA CBI H-24616: Static, Acute, 96-Hour Screening Test to Pimephalespromelas DuPont-6818 C E R T IF IC A T IO N We, the undersigned, declare that this report provides an accurate evaluation o f data obtained from this study. Work Completed by: c'T)amel J. Peterson, B.S. Associate Scientist if - iT# / y -- , 1 ^ / ' fiate Issued by Study Director: As6u/- / 1. Sfakt Robert A. Hoke, PLD. Senior Research Ecotoxicologist / / tZco/ Date Comoany Sanitized. Dees not contain TSCA CBf -2- H -24616: Static, Acute, 96-Hour Screening Test to Pimephalespromelas STUDY INFORM ATION Substance Tested:! DuPont-6818 Physical Characteristics: Opaque tan liquid Sponsor: E. I. du Pont de Nemours and Company Wilmington, Delaware 19898 U.S.A. Study Initiated/Completed : June 25,2001 / July 11,2001 In-Life Initiated/Completed: June 25,2001 / June 29,2001 Company Sanitized. Does not contain TSCA CB1 H -24616: Static, Acute, 96-Hour Screening T est to Pimephalespromelas DuPont-6818 SUMMARY The acute toxicity o f H-24616 to the fathead minnow, Pimephales promelas, was determined in an unaerated, 96-hour, static test. The study was conducted with 4 concentrations o f H-24616 and a dilution water control at a mean temperature o f 20.7C (range of20.7-20.8C). One test chamber was used per test concentration with 10 test organisms in each chamber. Based on visual observations, the water control and 1,10, and 100 mg/L test concentrations were clear and colorless at test start. The 1000 mg/L concentration was cloudy at test start. All water quality parameters were within acceptable limits during the exposure. Exposure o f fathead minnows to the water control and nominal H-24616 concentrations o f 1,10,100, and 1000 mg/L resulted in 0 ,0 ,0 ,0 , and 100% mortality, respectively, at the end o f 96 hours. No m ortality or sublethal effects were seen in the water control test organisms. The highest nominal concentration causing no mortality at test end was 100 mg/L. The lowest nominal concentration causing 100% mortality at test end was 1000 mg/L. Nominal H-24616 concentrations were used for calculation o f LC50 values. The results are summarized as follows: Nominal concentrations o f H-24616, mg/L 96-hour LC50 (95% confidence interval) for H-24616, based on nominal concentrations, mg/La dilution water control, 1,10,100, and 1000 316 (100 to 1000) H-24616 exhibited low concern for aquatic hazardb in an unaerated, 96-hour, static, acute test using the fathead minnow, Pimephales promelas. * Stephan, C.E. (1997). Methods for calculating an LC50. Aquatic Toxicology and Hazard Evaluation, Proceedings o fthe First Annual Symposium on Aquatic Toxicology, ASTM Special Publication 634 (F.L. Mayer and J.L. Hamelink, Eds.), pp 65-84. Philadelphia. b Smrchek, J., Clements, R., Morcock, R., and Rabert, W. (1993). Assessing ecological hazard under TSCA: methods and evaluation o f data. Environmental Toxicology and Risk Assessment, ASTM ST P 1179. (W.G. Landis, J.S. Hughes, and M.A. Lewis, Eds.), pp 22-39. American Society for Testing and Materials, Philadelphia, PA. Company Sanitized. Does not contain TSCA CBI -4 - H-24616: Static, Acute, 96-Hour Screening Test to Pimepkalespromelas D u P on t-6818 TABLE 1 DISSOLVED OXYGEN AND pH OF TEST AND CONTROL SOLUTION SAMPLES Nominal H-24616 Concentration (m g/Q Test Initiation (0 hours) Dissolved Oxygen (mg/L) pH Test Completion (96 hours) Dissolved Oxygen (mg/U)____ PH_____ W ater Control 9.0 7.4 7.7 7.4 1 9.0 7.3 6.9 7.3 10 9.0 7.3 7.2 7.3 100 9.0 7.2 7.3 7.3 1000 8.9 7.2 7.9s 7.2a a Measurement taken at 24 hours due to total mortality TABLE 2 MORTALITY OF Pimephales promelas IN AN UNAERATED, STATIC, ACUTE, 96-HOUR TEST WITH H-24616 N om inal H-24616 Concentration (mg/L) W ater Control 1 10 100 1000 24 Hours 0/10 0/10 0/10 0/10 10/10 Number Dead / Number at Study Start 48 Hours 72 Hours 0/10 0/10 0/10 0/10 10/10 0/10 0/10 0/10 0/10 10/10 96 Hours 0/10 0/10 0/10 0/10 10/10 Company Sanitized. Does not contain TSCA CB1 -5 -