Document q3jZevpGGpbpOJ8vB85qYb3gj

UNITED STATES AGENCY ENVIRONMENTA PROTECT REGION 1 NEW ENGLAND REGIONAL LABORATORY NORTH CHELMSFORD, MA 01863 MEMORANDUM July 1st, 2024 Subj:Concentrated Animal Feeding Operation (CAFO) Sampling at Veldman Farm From:Nafisah Ali, InspectorDigitally signed by Ali, Laboratory Services and Applied Science Division (LSASD)Ali, NaDfatie:s 2a0h24 .07.22 Field Services Branch (FSB2)Nafisah 12:07:01 -04'00 ' Reviewed By: Eric Magnan, ManagerERIC Digitally signed by ERIC MAGNAN LSASD - FSB2Date: 2024.07.22 MAGNAN 12:59:41 -04'00 ' To:Andrew Spejewski, Inspector Enforcement and Compliance Assurance Division (ECAD) Water Compliance Section 1 I. Facility Information A. Facility Name: Veldman Farm B. Facility Location: 6144 VT Route 22a. Vergennes, VT 05491 C. Facility Contact: N / A D. NPDES Permit ID: N / A II. Background Information A. Date / Start time of sampling inspection: 5/21/2024 12:41, 5/22/2024 08:25 B. Weather conditions: Sunny, 85 F C. USEPA Representatives: Andrew Spejewski, Eleanor Horvath, Nafisah Ali, Anna Hatke D. State / Local Representatives: Nate Sands, VT DEC E. Federally Enforceable Requirements Investigated: N / A III. Disclaimer: Unless otherwise noted, this report describes conditions at the facility / property as observed by EPA inspector(s), and / or through records provided to and / or information reported to EPA inspector(s) by facility representatives and as understood by the inspector(s). This report may not capture all operations or activities ongoing at the time of the inspection. This report does not make final determinations on ED_019088A_00004946-00001 EPA CAFO Compliance Sampling Inspection potential areas of concern. Nothing in this report affects EPA's authorities under federal statutes and regulations to pursue further investigation or action. IV. Type and Purpose of Sampling Inspection(s) The purpose of the compliance sampling inspection was to assess applicability of and compliance with the CAFO requirements under the Clean Water Act at 40 C.F.R. Part 122.23. V. Sampling Inspection Summary On Tuesday, May 21st and Wednesday, May 22nd, 2024, Nafisah Ali, and Anna Hatke of FSB 2 (" the EPA Sampling Team "), conducted wastewater sampling of three locations at Veldman Farm (see photos and descriptions included as Attachment 1). The sampling activities were conducted in accordance with the Standard Operating Procedure for the Collection of Chemical and Biological NPDES Water and Wastewater Samples FSBSOP - NPDES Water Revision 2, 2020. On Tuesday May 21st, 2024, the EPA sampling team collected one grab sample (" VF01 ") for Total Suspended Solids (TSS), Source Tracking Analysis ($ STA), Ammonia (AMMO), Total Nitrogen (TN) and Total Phophorous (TP). Total Suspended Solids sample were collected in a 1-liter (L) High Density Polyethylene (HDPE) bottle. Source Tracking Analysis sample was collected in a 1-L Amber glass teflon, lined cap bottle. Ammonia, Total Nitogen and Total Phophorous were collected in one 500 mL HDPE bottle with preservative Sulfuric Acid to attain a pH of < 2. Samples were split with the facility; the EPA sampling team provided the sample bottles. The samples collected by the EPA sampling team were then placed in coolers with ice to maintain an internal temperature of less than 4 degrees Celsius. On Wednesday May 22nd, 2024, the EPA sampling team collected two in - stream samples (VF02 and VF03) for E. coli (Colilert - MPN), Total Suspended Solids (TSS), Source Tracking Analysis ($ STA), Ammonia (AMMO), Total Nitrogen (TN) and Total Phophorous (TP). The E. coli samples were collected in 120mL UV Sterile, pre - preserved bottle with Sodium Thiosulfate. Total Suspended Solids samples were collected in a 1-liter (L) High Density Polyethylene (HDPE) bottle. Source Tracking Analysis samples were collected in a 1-L Amber glass teflon, lined cap bottle. Ammonia, Total Nitrogen and Total Phophorous were collected in one 500 mL HDPE bottle with preservative Sulfuric Acid to attain a pH of < 2. There were no split samples with the facility. The samples collected by the EPA sampling team were then placed in coolers with ice to maintain an internal temperature of less than 4 degrees Celsius. Samples will be analyzed for E. Coli, at Endyne Laboratory, 56 Etna Road Lebanon, NH 03766. Samples for TSS, $ STA, TN, and TP will be analyzed at the EPA New England Regional Laboratory in North Chelmsford, MA. VI. Description of Sampling Location (in order of collection time) Photographs depicting each sample location are included in Attachment 1 of this report. VF01: Grab sample from a stagnant deposition of water immediately downhill of a feed bunker with a concrete floor and no roof was collected at Veldman Farm (6146 VT-22A). The sample was collected approximately 3 feet from the Southern bank of the leachate run- off water as indicated by red X. A sweet, fermented odor was detected. Color was clear- yellow, and turbidity was minimal. Organic floatables were observed floating in the water. Specks of white foam was observed on Page 2 of 8 Sampling Inspection Date: 5 / 21 / 2024-5 / 22 / 2024 ED_019088A_00004946-00002 EPA CAFO Compliance Sampling Inspection top of the water. Grasses and small juvenile trees were observed growing in and around the stagnant water. VF02: In - stream sample of unnamed Brook discharging into Lake Champlain collected at 537-7 Basin Harbor Road. The sample was collected approximately 1 foot from the Southern bank of the stream approximately 50 feet upstream of a 2.5 foot - diameter PVC culvert as indicated with red X. No notable odor was detected. Color was clear - brown, and turbidity was low. Yellowish- white foam was observed collecting approximately 50 feet downstream before the culvert pipe. VF03: In - stream sample of an unnamed brook discharging into wetlands surrounding Lake Champlain collected at the intersection of Basin Harbor Road and Town Line Road. The sample was collected approximately half a foot from the Northern bank of the approximately 10 feet downstream of a PVC culvert pipe 1 foot in diameter as indicated by red X. No notable odor was detected. Color was clear - yellow, and turbidity was low. Organic matter including leaves and grass was observed floating on top of the water directly downstream of the culvert. VII. EPA Sampling and Analytical Results The table below provides a summary of the laboratory analytical results collected during the inspection. For a full list of compounds analyzed for each parameter and their results, refer to the laboratory reports that are attached to this inspection report (Attachment 2). Page 3 of 8 Sampling Inspection Date: 5 / 21 / 2024-5 / 22 / 2024 ED_019088A_00004946-00003 EPA CAFO Compliance Sampling Inspection Table 1: Summary of Veldman Farm (VF01, VF02 VF03) May 21ST - May 22nd, 2024, analytical results. Location Site ID VF01 VF02 VF03 Bacteria Sample date 5/21/24 5/22/24 5/22/24 Sample time 12:41 08:25 12:00 (MPN / 100mL) E. coliNC 920 420 Ammonia AMMO NA NA NA (mg / L) Total Nitrogen (ug / L) TN 1100 140 380 Total Phosphorous TP 3000 70 1100 (ug / L) TSS (mg / L) TSS45 5.3 37 PPCPs (ng / L) CotinineNC 0.56 3.4 Acetaminophen NC ND ND Paraxanthine NC ND 12 Atenolol NC ND 2.5 Caffeine NC ND 130 Metoprolol NC ND ND Diphenhydramine NC ND ND Carbamazepine NC ND ND * NC = Not Collected * NA Not Analyzed * ND = Not Detected Attachments: Attachment 1: Photo of sampling locations (VF01, VF02, F03) and arial image at Veldman Farm. Attachment 2: Laboratory Reports for Analysis (E. coli, TSS, $ STA, TN, TP) Note: Pages of Laboratory Reports have been redacted, the information that has been redacted does not pertain to this facility. Page 4 of 8Sampling Inspection Date: 5 / 21 / 2024-5 / 22 / 2024 ED_019088A_00004946-00004 EPA CAFO Compliance Sampling Inspection Attachment 1: Photo of sampling location VF01 and arial image at Veldman Farm. Page 5 of 8 Sampling Inspection Date: 5 / 21 / 2024-5 / 22 / 2024 ED_019088A_00004946-00005 EPA CAFO Compliance Sampling Inspection Photo of sampling location VF02 and arial image at Veldman Farm. Page 6 of 8 Sampling Inspection Date: 5 / 21 / 2024-5 / 22 / 2024 ED_019088A_00004946-00006 EPA CAFO Compliance Sampling Inspection Photo of sampling location VF03 and arial image at Veldman Farm. Page 7 of 8 Sampling Inspection Date: 5 / 21 / 2024-5 / 22 / 2024 ED_019088A_00004946-00007 EPA CAFO Compliance Sampling Inspection Attachment 2: Laboratory Reports for Analysis (E. coli, TSS, $ STA, TN, TP) Note: Pages of Laboratory Reports have been redacted, the information that has been redacted does not pertain to this facility. Redacted Information E. coli: Total of 4 pages TSS: Total of 7 pages TN: Total of 18 pages, pages 1,2,4,10-18 pertains to this facility. $ STA: Total of 15 pages, pages 1,2,7,8,9,10-15 pertains to this facility. TP: Total of 8 pages Page 8 of 8 Sampling Inspection Date: 5 / 21 / 2024-5 / 22 / 2024 ED_019088A_00004946-00008 ENDYNE Inc. Environmental Laboratories Page 1 of 2 Laboratory Report EPA 089621 11 Technology Drive Chelmsford, MA 01863. PROJECT: E. coli Analysis WORK ORDER: 2405-14444 DATE RECEIVED: May 22, 2024 DATE REPORTED: May 28, 2024 SAMPLER:Nafisah Ali Enclosed please find the results of the analyses performed for the samples referenced on the attached chain of custody located at the end of this report. The column labeled Lab / Tech in the accompanying report denotes the laboratory facility where the testing was performed and the technician who conducted the assay. A " W " designates the Williston, VT lab under NELAC certification ELAP 11263; " R " designates the Lebanon, NH facility under certification NH 2037 and " N " the Plattsburgh, NY lab under certification ELAP 11892. " Sub " indicates the testing was performed by a subcontracted laboratory. The accreditation status of the subcontracted lab is referenced in the corresponding NELAC and Qual fields. This NELAC column also denotes the accreditation status of each laboratory for each reported parameter. " A " indicates the referenced laboratory is NELAC accredited for the parameter reported. " N " indicates the laboratory is not accredited. " U " indicates that NELAC does not offer accreditation for that parameter in that specific matrix. Test results denoted with an " A " meet all National Environmental Laboratory Accreditation Program requirements except where denoted by pertinent data qualifiers. Endyne, Inc. warrants, to the best of its knowledge and belief, the accuracy of the analytical test results contained in this report, but makes no other warranty, expressed or implied, especially no warranties of merchantability or fitness for a particular purpose. Reviewed by: Alley Hart Alexander J Rakotz Laboratory Director Lebanon, NH www.endynelabs.com NELA ACCREDITEDCCREDI REDITRO 160 James Brown Dr., Williston, VT 0549556 Etna Road, Lebanon, NH 03766 Ph 802-879-4333Fax 802-879-7103Ph 603-678-4891 Fax 603-678-4893 RABORATOR KABORATORY ELAP 11263NH2037 ED_019088A_00004946-00009 Page 2 of 2 Laboratory ReportDATE REPORTED: 05/28/2024 CLIENT: EPAWORK ORDER: 2405-14444 PROJECT: E. coli AnalysisDATE RECEIVED:05/22/2024 001Site: VF 02Date Sampled: 5/22/24 Time: 8:25 ParameterResult Units Method Analysis Date / Time Lab / Tech NELAC Qualfdpijaj ajajajajajajajar E. coli920MPN / 100 mL SM20 9223B QuantiTray5/22/2415:54 RAJRA 002Site: VF 03Date Sampled: 5/22/24 Time: 12:00 ParameterResult Units Method Analysis Date / Time Lab / Tech NELAC Qual. E. coli420MPN / 100 mL SM20 9223B QuantiTray5/22/2415:54 RAJRA ENDYNE Inc. www.endynelabs.com ED_019088A_00004946-00010 E. coli AnalysisEndyne inc. COCIsh Ilse WO # Prepared: 1/31/23 2405-14444 Bill To:Report To: Cust # EPANafisah Ali 2405-14444 EPA 11 Technology Drive, Chelmsford 01863E. coli Analysis Ph:Email: ali. nafisah@epa.gov LOCATION VF 02Sampled Date / Time:5 / 22 / 24 @ 8: 25 Am Sampler: Nafisah Ali E. coli1-120ml Sterile Plastic< 10C, Na2S2O3 LOCATION VF 03Sampled Date / Time:5/22/24 @ 12:00 Sampler:NA E. coli1-120ml Sterile Plastic< 10C, Na2S2O3 $ 25.00 Per Sample * Samples MUST be received within 6 hours of sample collection *** ' Samples must be filled to the bottom of the bottle's cap where the lid screws on. Relinquished by:Accepted by: Date TimeDate Time Relinquished by:Received by: Sites / Parameters correct as listed. Client initials Date TimeDate Time Client Authorization to use Subcontract lab Client Initials Delv:Tmpl CkLab use Only Temp C:Log by Sample origin: VT NH NY OtherComment: Special reporting instructions:(PO #) Requested Turnaround Time: Routine: Rush Due Date 160 James Brown Dr.56 Etna Road315 New York Rd. ENDYNE Inc. Williston, VT 05495Lebanon N 03766Plattsburgh, NY 12903 Ph 802-879-4333Ph 603-678-4891Ph 518-563-1720 www.endynelabs.comFax 802-879-7103Fax 603-678-4893Fax 518-563-0052 Loft ED 019088A 00004946-00011 E. coli AnalysisEndyne Inc. COCLab Use WO # Bill To:Report To: Prepared: 1/31/23 Cust # 089621 GEN Ph:Email: ECOL! Page 1 of 1 LOCATIONSampled Date / Time:Sampler: E. coli1-120ml Sterile Plastic< 10C, Na2S203 LOCATIONSampled Date / Time:Sampler: E. coli1-120ml Sterile Plastic< 10C, Na2S203 LOCATION Sampled Date / Time:@Sampler: E. coli1-120ml Sterile Plastic< 10C, Na2S203 LOCATION Sampled Date / Time:@Sampler: E. coli1-120ml Sterile Plastic< 10C, Na2S203 $ 25.00 Per Sample ** Samples MUST be received within 6 hours of sample collection *** Samples must be filled to the bottom of the bottle's cap where the lid screws on. Relinquished by:Accepted by: Date TimeDate Time Relinquished by:Received by:AS 5/27/24 1530 Sites / Parameters correct as listed. Client Initials Date TimeDate Time Delv:Tmpl CkLab use Only Chant Authorization to use Subcontract fab Client Initials Temp C:6.ScaLog by Sample origins: VT NH NY Other Comment: Spacial reporting instructions:(PO #) Requested Turnaround Time: Routine: Rush Due Date 160 James Brown Dr.56 Eina Road315 New York Rd. Williston, VT 05495Lebanon, Ni: 03766Plattsburgh, NY 12003 ENDYNE Inc.Ph 802F-a8x7 980 -24-383739- 7P1h0 36F 0a3x- 667083--647889-1489P3h F5ax1 85-1586-536-31-7020502 www.endynelabs.com20f2 ED_019088A_00004946-00012 United States Environmental Protection Agency EPALaboratory Services and Applied Science DivisionPage 1 of 7 Region 1, New England11 Technology Drive North Chelmsford, MA 01863-2431 June 10, 2024 Laboratory Results Nafisah Ali US EPA Region 1 Project No:24050033 Project:Concentrated Animal Feeding Operation Sampling Inspectio Analysis:Total Suspended Solids in Water EPA Chemist:Holly Westbrook Date Samples Received by the Laboratory: 05/23/2024 Analytical Procedure: All samples were received and logged in by the laboratory according to the USEPA New England Laboratory SOP for Sample Log - in. Sample preparation and analysis was done following the EPA Region I SOP, LSBSOP - TSS - TDS7. The SOP is based on SM 2540 D. Results relate only to the items tested or to the samples as received by the Laboratory. This analytical report shall not be reproduced except in full, without written approval of the laboratory. Data were reviewed in accordance with the internal verification procedures described in the EPA New England Quality Manual for NERL. If you have any questions please call me at 617-918-8340. Sincerely, DANIEL Digitally signed by DANIEL BOUDREAU BOUDREAU D1a3t:e21:: 2220 2-404.'0060. 1' 0 24050033TSS ED_019088A_00004946-00013 Qualifiers: Page 2 of 7 RL = Reporting limit ND = Not Detected above Reporting limit NA = Not Applicable due to high sample dilutions or sample interferences NC = Not calculated since analyte concentration is ND. J = Estimated value J1 = Estimated value due to MS recovery outside accceptance criteria J2 = Estimated value due to LFB result outside acceptance criteria J3 = Estimated value due to RPD result outside acceptance criteria J4 = Estimated value due to LCS result outside acceptance criteria E-Estimated value exceeds the calibration range L = Estimated value is below the calibration range B = Analyte is associated with the lab blank or trip blank contamination. Values are qualified when the observed concentration of the contamination in the sample extract is less than 10 times the concentration in the blank. R = No recovery was calculated since the analyte concentration is greater than four times the spike level. P = The confirmation value exceeded 35% difference and is less than 100%. The lower value is reported. C = The identification has been confirmed by GC / MS. A = Suspected Aldol condensation product. N Tentatively identified compound. ANR Analysis not required. 24050033TSS ED_019088A_00004946-00014 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORY Page 3 of 7 Concentrated Animal Feeding Operation Sampling Inspection Matrix: Wastewater Total Suspended Solids in Water Sample Number Lab ID Collected Analysis Concentration RL mg / L mg / L Qualifier VF01 AC13045 05/22/2024 12:41 05/29/2024 45 2.5 Comments: VF02 AC13046 05/22/2024 8:25 05/28/2024 5.3 2.5 Comments: VF03 AC13047 05/22/2024 12:00 05/29/2024 37 2.5 Comments: Blank Comments: 05/28/2024 ND 2.5 24050033TSS ED_019088A_00004946-00015 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORY Page 4 of 7 Concentrated Animal Feeding Operation Sampling Inspection 24050033TSS ED_019088A_00004946-00016 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORY Page 5 of 7 Concentrated Animal Feeding Operation Sampling Inspection Laboratory Duplicate Results SAMPLE SAMPLE DUP PRECISION QC RESULT RESULT RPD LIMITS SAMPLE ID PARAMETER mg / L mg / L% (% RPD) AC13039 Total Suspended Solids in Water 7.0 6.5 7.4 25 24050033TSS ED_019088A_00004946-00017 Page 6 of 7 US EPA Region 1CHAIN OF CUSTODY RECORDPage: of 11 Technology Drive, N. Chelmsford, MA 01863 PROTECTION EPA Project No:Project Name & Location:[Sampler's Name and Signature: 24050033. Concentrated Animal Feeding Operation (CAFO) Sampling Inspection Nafisah Ali / Anna Hatke Matrix (insert letter in column below)Preservative (insert # in box to the right) - -2 sejdwes For EPA Staff Only: If after A Drinking waterF. Soil / Sediment / Solid 1. Ice6. NaOH p# h 12Analysis Requestedhours or nobody is available, B. Ground / Well water G. Oil / Solvent / Liquid 2.F50pH <of 2 7. Ascorbic Acid.leave cooler(s) and signed COC C. Storm waterH. Soil Gas3. HNO3, PH>2: 8. MeOHin the walk - in fridge (Room 190A) NO2N03 D. Surface water1. Air Canister4. HCl, pH>29. Otherand notify appropriate staff for E. WasStaemwpaltee rIDJDa5t.e NTai2mSe2 OCo3mp1 0G.r aObt hMaetrriCxonAtmamio nSeT$r As T/SSneSaxmtp ldea yS pcehceifcikc -C oimnm.e nts of Containers VF01 05/21/2024 12:41 2 VF02 05/22/2024 08:25 E VF03 05/22/2024 12:00. E Relinquished by: (Signature)Received by: (Signature)DateTimeRemaAq rks Ali, Nafisah DigDaite:t 2a024l05l.2y2 1 8:s57i:23g -n04e'00d ' by Ali, Nafisah Laura Glowacko (ESAT) 5/23/24 8:58 CAFO samples collected at Relinquished by: (Signature)Received by: (Signature) DateTime different farms. Distribution: Original Accompanies Shipment. Copy to Coordinator Field Files,FSBFORM CHAINCUSTODY1 Revision 1 ED_019088A_00004946-00018Relinquished by: (Signature)Received for Laboratory by: (Signature)[DateTime 05/16/2022 ED_019088A_00004946-00018 Page 7 of 7 US EPA REGION 1 SAMPLE RECEIPT CHECKLIST PROJ #: 24050033RECEIPT DATE: 5/23/24 LSOUCRAVTEIYO NN:A ME: Concentrated Animal Feeding OperationREC'D BYL:a ura Glowacki (ESAT). OSC / PO: Nafisah AliSITE ID:SUPERFUND: N - WERE SAMPLES SHIPPED?COMMENTS: TRACKING #: DATE / SENTWastewater samples NO Hand Delivered COOLER TEMPERATURE UPON ARRIVAL 21 C / NA 9 $ TNL CHAIN OF CUSTODY PRESENT? Y9 AMMO COMPLETE? Y 9 TP CUSTODY SEALS PRESENT ON COOLER?N SAMPLES? N WERE SAMPLE CONTAINERS INTACT? 9 TSS 8. $ STA WAS SAMPLE PRESERVATION DOCUMENTED?YSamples relinquished to walk in COCSample Containeron 5/22/24 by Nafisah Ali and recieved by Laura Glowacki on APPROPRIATE SAMPLES VOLUME5/23/24 FOR REQUESTED ANALYSIS?Y SAMPLES AND COC MATCH?Y IF ANY PROBLEMS WAS PROJECT MANAGER NOTIFIED? BY WHOM? NA Y APPROPRIATE SAMPLE CONTAINERS? : SAMPLES WITHIN HOLDING TIMES?Y ALL ANALYSIS SPECIFIED ON COC?Y DATE / TIME OF COLLECTION ON COCY TURN - AROUND TIME: 4 weeks ED_019088A_00004946-00019 United States Environmental Protection Agency EPALaboratory Services and Applied Science DivisionPage 1 of 18 Region 1, New England11 Technology Drive North Chelmsford, MA 01863-2431 June 24, 2024 Laboratory Report Nafisah Ali US EPA Region 1 Project Number: 24050033 Project: Concentrated Animal Feeding Operation Sampling Inspectic Analysis: Total Nitrogen EPA Chemist: Paul Toompas Date Samples Received by the Laboratory: 05/23/2024 Analytical Procedure: All samples were received and logged in by the laboratory according to the USEPA New England Laboratory SOP for Sample Log - in. Sample preparation and analysis was done following the EPA Region I SOP, LSBSOP - NO2 - NO34. Combined nitrate and nitrite will be reported as nitrogen. Total nitrogen (the sum of nitrate - nitrogen, nitrite - nitrogen, ammonia - nitrogen and organically bonded nitrogen) will be reported as nitrogen. Samples were analyzed according to LSBSOP - NO2 - NO34, which is currently under review. Samples were analyzed on a SEAL AQ300 Discrete Analyzer. Data were reviewed in accordance with the internal verification procedures described in the EPA New England Quality Manual for NERL. Results relate only to the items tested or to the samples as received by the Laboratory. This analytical report shall not be reproduced except in full, without written approval of the laboratory. If you have any questions please call me at 617-918-8340. Sincerely, DANIEL Digitally signed by DANIEL BOUDREAU BOUDREAU Date: 2024.06.2424050033 $ TNL 10:42:43 -04'00 ' ED_019088A_00004946-00020 Qualifiers: RL = Reporting limit ND = Not Detected above Reporting limit NA = Not Applicable due to high sample dilutions or sample interferences NC = Not calculated since analyte concentration is ND. J = Estimated value J1 = Estimated value due to MS recovery outside accceptance criteria J2 = Estimated value due to LFB result outside acceptance criteria J3 = Estimated value due to RPD result outside acceptance criteria J4 = Estimated value due to LCS result outside acceptance criteria E = Estimated value exceeds the calibration range L = Estimated value is below the calibration range B = Analyte is associated with the lab blank or trip blank contamination. Values are qualified when the observed concentration of the contamination in the sample extract is less than 10 times the concentration in the blank. R = No recovery was calculated since the analyte concentration is greater than four times the spike level. P = The confirmation value exceeded 35% difference and is less than 100%. The lower value is reported. C = The identification has been confirmed by GC / MS. A = Suspected Aldol condensation product. N = Tentatively identified compound. ANR = Analysis not required. Page 2 of 18 24050033 $ TNL ED_019088A_00004946-00021 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORYPage 10 of 18 Concentrated Animal Feeding Operation Sampling Inspection Total Nitrogen Client Sample ID:VF01Lab Sample ID:AC13045 Date of Collection:5/22/2024Matrix:Wastewater Date of Preparation:6/12/2024Amount Prepared: N / A Date of Analysis:6/12/2024Percent Solids:N / A Dry Weight Prepared:N / AExtract Dilution:I Wet Weight Prepared:N / ApH:S2 Volume Extracted:N / AGPC Factor:N / A Final Volume:N / A Concentration RL CAS Number Compoundug / L ug / L Qualifier Combined Nitrate & Nitrite110040J Comments: Sample not preserved to a pH less than 2. 24050033 $ TNL ED_019088A_00004946-00022 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORYPage 11 of 18 Concentrated Animal Feeding Operation Sampling Inspection Total Nitrogen Client Sample ID:VF02Lab Sample ID:AC13046 Date of Collection:5/22/2024Matrix:Wastewater Date of Preparation:6/12/2024Amount Prepared: N / A. Date of Analysis:6/12/2024Percent Solids:N / A Dry Weight Prepared:N / AExtract Dilution:I Wet Weight Prepared:N / ApH:> 2 Volume Extracted:N / AGPC Factor:N / A Final Volume:N / A Concentration RL CAS Number Compoundug / L ug / L Qualifier Combined Nitrate & Nitrite14040J Comments: Sample not preserved to a pH less than 2. 24050033 $ TNL ED_019088A_00004946-00023 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORYPage 12 of 18 Concentrated Animal Feeding Operation Sampling Inspection Total Nitrogen Client Sample ID:VF03Lab Sample ID:AC13047 Date of Collection:5/22/2024Matrix:Wastewater Date of Preparation:6/12/2024Amount Prepared: N / A Date of Analysis:6/12/2024Percent Solids:N / A Dry Weight Prepared:N / AExtract Dilution:I Wet Weight Prepared:N / ApH:> 2 Volume Extracted:N / AGPC Factor:N / A Final Volume:N / A Concentration RL CAS Number Compound ug / L ug / L Qualifier Combined Nitrate & Nitrite38040J Comments: Sample not preserved to a pH less than 2. 24050033 $ TNL ED_019088A_00004946-00024 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORYPage 4 of 18 Concentrated Animal Feeding Operation Sampling Inspection Laboratory Blank for Nitrogen Client Sample ID:N / ALab Sample ID:N / A Date of Collection:N / AMatrix:Wastewater Date of Preparation:6/12/2024Amount Prepared: N / A Date of Analysis:6/12/2024Percent Solids:N / A Dry Weight Prepared:N / AExtract Dilution:N / A Wet Weight Prepared:N / ApH:n / a Volume Extracted:N / AGPC Factor:N / A Final Volume:N / A Concentration RL CAS Number Compound ug / L ug / L Qualifier Combined Nitrate & NitriteND40 ** 24050033 $ TNL ED_019088A_00004946-00025 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORYPage 13 of 18 Concentrated Animal Feeding Operation Sampling Inspection MATRIX SPIKE (MS) RECOVERY Sample ID: AC13039 SPIKE SAMPLE MS MS QC ADDED CONCENTRATION CONCENTRATION% LIMITS PARAMETER ug / L ug / L ug / L REC (% REC) Combined Nitrate & Nitrite40034404010R80-120 24050033 $ TNL ED_019088A_00004946-00026 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORY Page 14 of 18 Concentrated Animal Feeding Operation Sampling Inspection MATRIX SPIKE DUPLICATE (MSD) RECOVERY Sample ID: AC13039 MSD MSD MSD RPD QC SPIKE CONCENTRATION%% LIMITS PARAMETER ADDED ug / L REC RPD Combined Nitrate & Nitrite 400 4440 R 10.2 20 24050033 $ TNL ED_019088A_00004946-00027 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORY Page 15 of 18 Concentrated Animal Feeding Operation Sampling Inspection Laboratory Duplicate Results Sample ID: AC13039 SAMPLE SAMPLE DUPLICATE PRECISION RESULT RESULT RPD QC PARAMETER ug / L ug / L% LIMITS Combined Nitrate & Nitrite 3440 3280 S 20 24050033 $ TNL ED_019088A_00004946-00028 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORYPage 16 of 18 Concentrated Animal Feeding Operation Sampling Inspection Laboratory Fortified Blank (LFB) Results LFB AMOUNT LFB LFB QC SPIKED RESULT RECOVERY LIMITS PARAMETER ug / L ug / L%% Combined Nitrate & Nitrite4003939890-110 Comments: Samples in Batch:AC13039, AC13040, AC13041, AC13042, AC13043, AC13044, AC13045, AC13046, AC13047 24050033 $ TNL ED_019088A_00004946-00029 S . TATE #Page 17 of 18 US EPA Region 1CHAIN OF CUSTODY RECORDPage: of 11 Technology Orive, N. Chelmsford, MA 01863 EPA Profect No:Project Name & Location:Sampler's Name and Signature: 24050033 Concentrated Animal Feeding Operation (CAFO) Sampling Inspection Nafisah Ali / Anna Hatke Matrix (insert letter in column below)Preservative (insert # in box to the right) - >2 For EPA Staff Only: If after A. Drinking waterF. Soil / Sediment / Solid1. Ice6. Nach, #p hi 12Analysis Requestedhours or nobody is available, B. Ground / Well waterG. Oil / Solvent / Liquid2. HSO,, pH < 27. Ascorbic Acidofleave cooler(s) and signed COC C. Storm waterH. Soil Gas3. HNO3, pH < 28. MeOHin the walk - in fridge (Room 190A) D. Surface water1. Air Canister4. HCl, pH < 29. Otherand notify appropriate staff for NO2N03 / E. WastewaterJ.5. NaSO10. Other Containersnext day check - in. Sample IDDateTime CompGrabMaAtmmor TSSi SSxTA Sample Specific Comments VF0105/21/2024 12:41 VVFF0023 050/52/2/2220/224 00284: 251 2w:w0w 0E. 2E00 33 Relinquished by: (Signature}Received by: (Signature)DateTimeRemarks Ali, NafisahDigDaite:t 2a024l.0l5.2y2 18:s 5i7: g23n-04e'0d0 " by Ali, NafisahLaura Glowacks (ESAT) 5/23/24 8:58 CAFO samples collected at Relinquished by: (Signature)Received by: (Signature) Date different farms. Relinquished by: (Signature)Received for Laboratory by: (Signature)DateTime Distribution: Original Accompanies Shipment. Copy to Coordinator Field Files.ESBFORM - CHAINCUSTODY1 Revision 1 05/16/2022 ED_019088A_00004946-00030 US EPA REGION 1 SAMPLE RECEIPT CHECKLIST PROJ #: 24050033receipt date: 5/23/24 SURVEY NAM LOCATION: E: Co nce nt rat ed An im al Fe eding Opera tion REC'D BY: Laura Glowacki (ESAT) OSC / PO: Nafisah AliSITE ID:SUPERFUND: N WERE SAMPLES SHIPPED?COMMENTS: TRACKING # DATE / SENT:Wastewater samples NO. Hand Delivered COOLER TEMPERATURE UPON ARRIVAL 2 C / NA 9 $ TNL CHAIN OF CUSTODY PRESENT? Y9 AMMO COMPLETE? Y 9 TP CUSTODY SEALS PRESENT ON COOLER? SAMPLES? WERE SAMPLE CONTAINERS INTACT?> 9 TSS 8. $ STA WAS SAMPLE PRESERVATION DOCUMENTED?Samples relinquished to walk in COCSample Containeron 5/22/24 by Nafisah Ali and recieved by Laura Glowacki on APPROPRIATE SAMPLES VOLUME5/23/24 FOR REQUESTED ANALYSIS? SAMPLES AND COC MATCH? IF ANY PROBLEMS WAS PROJECT MANAGER NOTIFIED? BY WHOM? Y APPROPRIATE SAMPLE CONTAINERS? SAMPLES WITHIN HOLDING TIMES?Y ALL ANALYSIS SPECIFIED ON COC?Y DATE / TIME OF COLLECTION ON COCY TURN - AROUND TIME: 4 weeks Page 18 of 18 ED_019088A_00004946-00031 United States Environmental Protection Agency RegiEoPnA 1L,a bNoerwa tEonrgyl aSnedrv1i1c eTse cahnndo lAoppglyi eDdr iSvcei ence DivisionPage 1 of 15 North Chelmsford, MA 01863-2431 June 10, 2024 Laboratory Report Nafisah Ali US EPA Region 1 Project Number: 24050033 Project:Concentrated Animal Feeding Operation Sampling Inspection Analysis: HPLC / MS / MS Source Tracking Analysis EPA Chemist: Peter Philbrook Date Samples Received by the Laboratory: 05/23/2024 Analytical Procedure: All samples were received and logged in by the laboratory according to the USEPA New England Laboratory SOP for Sample Log - in. Sample preparation and analysis was done following the EPA Region I SOP, LSBSOP - LCMS_STA3. The SOP is based on an EPA Regional Analytical Method developed at the EPA New England Laboratory. Data were reviewed in accordance with the internal verification procedures described in the EPA New England Quality Manual for NERL. Results relate only to the items tested or to the samples as received by the Laboratory. This analytical report shall not be reproduced except in full, without written approval of the laboratory. If you have any questions please call me at 617-918-8340. Sincerely, DANIEL Digitally signed by DANIEL BOUDREAU BOUDREAU Date: 2024.06.1024050033 $ STA 09: 08: 18-04'00 ' ED_019088A_00004946-00032 Qualifiers: RL = Reporting limit ND = Not Detected above Reporting limit NA = Not Applicable due to high sample dilutions or sample interferences NC = Not calculated since analyte concentration is ND. J = Estimated value JI = Estimated value due to MS recovery outside accceptance criteria J2 = Estimated value due to LFB result outside acceptance criteria J3 = Estimated value due to RPD result outside acceptance criteria J4 = Estimated value due to LCS result outside acceptance criteria E = Estimated value exceeds the calibration range L = Estimated value is below the calibration range B = Analyte is associated with the lab blank or trip blank contamination. Values are qualified when the observed concentration of the contamination in the sample extract is less than 10 times the concentration in the blank. R = No recovery was calculated since the analyte concentration is greater than four times the spike level. P The confirmation value exceeded 35% difference and is less than 100%. The lower value is reported. C = The identification has been confirmed by GC / MS. A = Suspected Aldol condensation product. N = Tentatively identified compound. Page 2 of 15 24050033 $ STA ED_019088A_00004946-00033 U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION I LABORATORY SERVICES BRANCHPage 7 of 15 Concentrated Animal Feeding Operation Sampling Inspection HPLC / MS / MS Source Tracking Analysis Client Sample ID:VF02Lab Sample ID:AC13046 Date of Collection:5/22/2024Matrix:Wastewater Date of Preparation:5/28/2024Amount Prepared: 500 mL Date of Analysis:6/04/2024Percent Solids:N / A Dry Weight Prepared:N / AExtract Dilution:I Wet Weight Prepared:N / ApH:N / A Concentration RL CAS Number Compound ng / L Dilution ng / L Qualifier 486-56-6 Cotinine 0.56 0.40 103-90-2 Acetaminophen ND 2.0 611-59-6 Paraxanthine ND 2.0 29122-68-7 Atenolol ND 2.0 58-08-2 Caffeine ND 1 4.0 56392-17-7 Metoprolol ND 1 2.0 58-73-1 Diphenhydramine ND 1 0.40 298-46-4 Carbamazepine ND 0.40 Surrogate CompoundsRecoveries (%)QC Ranges Phentermine - DS73 37-113 Diphenhydramine - D365 8-114 Comments: 24050033 $ STA ED_019088A_00004946-00034 U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION I LABORATORY SERVICES BRANCHPage 9 of 15 Concentrated Animal Feeding Operation Sampling Inspection HPLC / MS / MS Source Tracking Analysis Client Sample ID:VF03Lab Sample ID:AC13047 Date of Collection:5/22/2024Matrix:Wastewater Date of Preparation:5/28/2024Amount Prepared: 500 mL Date of Analysis:6/04/2024Percent Solids:N / A Dry Weight Prepared:N / AExtract Dilution: Wet Weight Prepared:N / ApH:N / A Concentration RL CAS Number Compound ng / L Dilution ng / L Qualifier 486-56-6 Cotinine 3.4 I 0.40 103-90-2 Acetaminophen ND 1 2.0 611-59-6 Paraxanthine 12 1 2.0 29122-68-7 Atenolol 2.5 1 2.0 58-08-2 Caffeine 130 4.0 56392-17-7 Metoprolol ND 2.0 58-73-1 Diphenhydramine ND 0.40 298-46-4 Carbamazepine ND 0.40 Surrogate CompoundsRecoveries (%)QC Ranges Phentermine - D568 37-113 Diphenhydramine - D357 8-114 Comments: 24050033SSTA ED_019088A_00004946-00035 U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION I LABORATORY SERVICES BRANCHPage 8 of 15 Concentrated Animal Feeding Operation Sampling Inspection Laboratory Blank Client Sample ID:N / ALab Sample ID:N / A Date of Collection:N / AMatrix:Wastewater Date of Preparation:5/28/2024Amount Prepared: 500 mL Date of Analysis:6/04/2024Percent Solids:N / A Dry Weight Prepared:N / AExtract Dilution: Wet Weight Prepared:N / ApH:N / A Concentration RL CAS Number Compound ng / L Dilution ng / L Qualifier 486-56-6 Cotinine ND 1 0.40 103-90-2 Acetaminophen ND 1 2.0 611-59-6 Paraxanthine ND 1 2.0 29122-68-7 Atenolol ND 1 2.0 58-08-2 Caffeine ND 4.0 56392-17-7 Metoprolol ND 2.0 58-73-1 Diphenhydramine ND 0.40 298-46-4 Carbamazepine ND 0.40 Surrogate CompoundsRecoveries (%)QC Ranges Phentermine - D510037-113 Diphenhydramine - D383 8-114 Comments: 24050033 $ STA ED_019088A_00004946-00036 U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION I LABORATORY SERVICES BRANCH Concentrated Animal Feeding Operation Sampling Inspection MATRIX SPIKE (MS) RECOVERY Sample ID: AC13047 Page 10 of 15 SPIKE SAMPLE MS MS QC ADDED CONCENTRATION CONCENTRATION% LIMITS PARAMETER ng / L ng / L ng / L REC (% REC) Acetaminophen 120 ND 99.3 * 83 55-99 Atenolol 120 2.5 100 81 * 24-160 Caffeine 240 129 337 87 * 34-131 Carbamazepine 24.0 ND 21.7 90 8 61-107 Cotinine 24.0 3.4 22.9 81 * 48-112 Diphenhydramine 24.0 ND 16.6 8 69 17-112 Metoprolol 120 ND 94.2 3 79 31-137 Paraxanthine 120 12.0 84.5 60 8 41-132 24050033 $ STA ED_019088A_00004946-00037 U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCHPage 11 of 15 Concentrated Animal Feeding Operation Sampling Inspection Laboratory Duplicate Results Sample ID: AC13046 SAMPLE SAMPLE DUPLICATE PRECISION RESULT RESULT RPD QC PARAMETER ng / L ng / L% LIMITS Acetaminophen ND ND NC 50 Atenolol ND ND NC 50 Caffeine ND ND NC 50 Carbamazepine ND ND NC 50. Cotinine 0.55994 0.662 17 50 Diphenhydramine ND ND NC 50 MPeatroa px ra on lt oh li n Ne D NNDD NN CD 8N 8C 8 5 50 0 24050033 $ STA ED_019088A_00004946-00038 U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION I LABORATORY SERVICES BRANCHPage 12 of 15 Concentrated Animal Feeding Operation Sampling Inspection Laboratory Fortified Blank (LFB) Results LFB AMOUNT LFB LFB QC SPIKED RESULT RECOVERY LIMITS PARAMETER ng / L ng / L%% Acetaminophen 120 92.2 77 45-106 Atenolol 120 105 88 33-129 Caffeine 240 189 79 41-120 Carbamazepine 24 20.4 85 63-108 Cotinine 24 18.5 77 55-106 Diphenhydramine 24 19.0 79 15-113 Metoprolol 120 110 92. 41-122 Paraxanthine 120 103 86 52-115 Comments: Samples in Batch:AC13039, AC13040, AC13041, AC13044, AC13046, AC13047 24050033 $ STA ED_019088A_00004946-00039 Page 14 of 15 US EPA Region 1CHAIN OF CUSTODY RECORDPage:of TAL 11 Technology Drive, N. Chelmsford, MA 01863 EPA Project No:[Project Name & Location:Sampler's Name and Signature: 24050033 Concentrated Animal Feeding Operation (CAFO) Sampling Inspection Nafisah Ali / Anna Hatke Matrix (insert letter in column below)Preservative (insert # in box to the right)2 For EPA Staff Only: If after A. Drinking waterF. Soll / Sediment / Solid1. Ice6. NaOH p# hi 12Analysis Requestedhours or nobody is available, B. Ground / Well waterG. Oil / Solvent / Liquid2. HSO,of pH < 27. Ascorbic Acidleave cooler(s) and signed COC C. Storm waterH. Soll Gas3. HNO3. pH < 28. MeOHin the walk - in fridge (Room 190A) D. Surface water1. Air Canister4. HCl, pH>29. Otherand notify appropriate staff for NO2N03 E. WastewaterJ.5. Na2S2O310. Other/ Containersnext day check - in. Sample IDDateTime Comp Grab MatrixAmmo S$T ATSSSample Specific Comments VF0105/21/2024 12:41 VVFF0032 0055/2/22/22/02204 2142 :0080: 25 E 200 3 Relinquished by: (Signature)Received by: (Signature)DateTimeRemarks Ali, NafisahDigDaite:t 2a02l4.l05y.22 1s8:i57g:2n3 -e04d00 by Ali, NafisahLaura Glowacks (ESAT) 5/23/24 8:58 CAFO samples collected at Relinquished by: (Signature)Received by: (Signature)DateTime different farms. Relinquished by: (Signature)Received for Laboratory by: (Signature)DateTime Distribution: Original Accompanies Shipment Copy to Coordinator Field Files.FSBFORM - CHAINCUSTODY1 Revision 1 05/16/2022 ED_019088A_00004946-00040 US EPA REGION 1 SAMPLE RECEIPT CHECKLIST PROJ #: 24050033RECEIPT DATE: 5/23/24 SURVEY NAME: Concentrated Animal Feeding OperationREC'D BY: LOCATION: Laura Glowacki (ESAT) osc / Po: Nafisah AliSITE ID:SUPERFUND: N WERE SAMPLES SHIPPED?COMMENTS: TRACKING #: DATE SENT:Wastewater samples NO. Hand Delivered COOLER TEMPERATURE UPON ARRIVAL 2C / NA 9 $ TNL CHAIN OF CUSTODY PRESENT? 9 AMMO COMPLETE?9 TP CUSTODY SEALS PRESENT ON COOLER?N SAMPLES?N WERE SAMPLE CONTAINERS INTACT? 9 TSS 8. $ STA WAS SAMPLE PRESERVATION DOCUMENTED?YSamples relinquished to walk in COCSample Containeron 5/22/24 by Nafisah Ali and recieved by Laura Glowacki on APPROPRIATE SAMPLES VOLUME5/23/24 FOR REQUESTED ANALYSIS?Y SAMPLES AND COC MATCH?Y IF ANY PROBLEMS WAS PROJECT MANAGER NOTIFIED? BY WHOM? Y APPROPRIATE SAMPLE CONTAINERS? SAMPLES WITHIN HOLDING TIMES?Y ALL ANALYSIS SPECIFIED ON COC?Y DATE TIME OF COLLECTION ON COCY TURN - AROUND TIME: 4 weeks Page 15 of 15 ED_019088A_00004946-00041 United States Environmental Protection Agency EPALaboratory Services and Applied Science DivisionPage 1 of 8 Region 1, New England11 Technology Drive North Chelmsford, MA 01863-2431 June 24, 2024 Laboratory Results Nafisah Ali US EPA Region 1 Project No:24050033 Project:Concentrated Animal Feeding Operation Sampling Inspectic Analysis:Total Phosphorus in Water as P EPA Chemist:Holly Westbrook Date Samples Received by the Laboratory: 05/23/2024 Analytical Procedure: All samples were received and logged in by the laboratory according to the USEPA New England Laboratory SOP for Sample Log - in. Sample preparation and analysis was done following the EPA Region I SOP, LSBSOP - OPHOS - TP13. The SOP is based on the AQ300 Method EPA-119-D Rev. 3A " Phosphorus - P, Total, in Surface and Saline Waters, Domestic and Industrial Wastes " and EPA method 365.1 " Determination of Phosphorous by Semi - Automated Colorimetry ", Revision 2.0, August 1993. Samples were analyzed on a SEAL AQ300 Discrete Analyzer. Results relate only to the items tested or to the samples as received by the Laboratory. This analytical report shall not be reproduced except in full, without written approval of the laboratory. Data were reviewed in accordance with the internal verification procedures described in the EPA New England Quality Manual for NERL. If you have any questions please call me at 617-918-8340. Sincerely, DANIEL Digitally signed by DANIEL BOUDREAU BOUDREAU Date: 2024.06.2424050033TP 11: 19: 07-04'00 ' ED_019088A_00004946-00042 Qualifiers: Page 2 of 8 RL = Reporting limit ND = Not Detected above Reporting limit NA = Not Applicable due to high sample dilutions or sample interferences NC = Not calculated since analyte concentration is ND. J = Estimated value J1 = Estimated value due to MS recovery outside accceptance criteria J2 = Estimated value due to LFB result outside acceptance criteria J3 = Estimated value due to RPD result outside acceptance criteria J4 = Estimated value due to LCS result outside acceptance criteria E = Estimated value exceeds the calibration range L Estimated value is below the calibration range B = Analyte is associated with the lab blank or trip blank contamination. Values are qualified when the observed concentration of the contamination in the sample extract is less than 10 times the concentration in the blank. R = No recovery was calculated since the analyte concentration is greater than four times the spike level. P The confirmation value exceeded 35% difference and is less than 100%. The lower value is reported. C = The identification has been confirmed by GC / MS. A = Suspected Aldol condensation product. N = Tentatively identified compound. ANR Analysis not required. 24050033TP ED_019088A_00004946-00043 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORY Concentrated Animal Feeding Operation Sampling Inspection Page 3 of 8 Total Phosphorus in Water as P Matrix:Wastewater Concentration RL Sample Number Lab ID Collected Extracted Analysisug / L ug / L Qualifier VF01AC13045 05/22/2024 12:41 06/17/2024 06/17/20243000 360 J Comments: Result determined from 30x dilution, reporting limit raised accordingly. Sample not preserved, results qualified. VF02AC13046 05/22/2024 8:25 06/17/2024 06/18/202470 12 J Comments: Sample not preserved, results qualified. VF03AC13047 05/22/2024 12:00 06/17/2024 06/17/20241100 120 J Comments: Result determined from 10x dilution, reporting limit raised accordingly. Sample not preserved, results qualified. Blank06/17/2024 06/17/2024 ND 12 Comments: 24050033TP: ED_019088A_00004946-00044 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORY Concentrated Animal Feeding Operation Sampling Inspection MATRIX SPIKE (MS) Results Page 4 of 8 SPIKE SAMPLE MS MS OC ADDED CONCENTRATION CONCENTRATION% LIMITS SAMPLE ID PARAMETERug / Lug / Lug / L REC (% REC) AC13040Total Phosphorus in Water as P8001551233497.983-119 Comments: MATRIX SPIKE DUPE (MSD) RESULTS MSD MSD MSD RPD QC SPIKE CONCENTRATION%% LIMITS SAMPLE ID PARAMETER ADDED ug / L REC RPD AC13040Total Phosphorus in Water as P800224086.120 Comments: 24050033TP ED_019088A_00004946-00045 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORY Page 5 of 8 Concentrated Animal Feeding Operation Sampling Inspection Laboratory Duplicate Results SAMPLE SAMPLE DUP PRECISION QC RESULT RESULT RPD LIMITS SAMPLE ID PARAMETER ug / L ug / L% (% RPD) AC13040 Total Phosphorus in Water as P 1551 1662 7 20 24050033TP ED_019088A_00004946-00046 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORYPage 6 of 8 Concentrated Animal Feeding Operation Sampling Inspection Laboratory Fortified Blank (LFB) Results LFB AMOUNT LFB LFB QC SPIKED RESULT RECOVERY LIMITS PARAMETER ug / L ug / L%% Total Phosphorus in Water as P8082.8103.590-110 Samples in Batch: AC13039, AC13040, AC13041, AC13042, AC13043, AC13044, AC13045, AC13046, AC13047 24050033TP ED_019088A_00004946-00047 Page 7 of 8 US EPA Region 1CHAIN OF CUSTODY RECORDPage:of 11 Technology Drive, N. Chelmsford, MA 01863 CPA Project No:Project Name & Location:Sampler's Name and Signature: 24050033 Concentrated Animal Feeding Operation (CAFO) Sampling Inspection Nafisah Ali / Anna Hatke Matrix (insert letter in column below)Preservative (insert # in box to the right) -2 For EPA Staff Only: If after A. Drinking waterF. Soil / Sediment / Solid1. Ice6 NaOH p# r 12Analysis Requestedhours or nobody is available, B. Ground / Well waterG. OV / Solvent / Liquid2. HSO, pH < 27. Ascorbic Acidofleave cooler(s) and signed COC C. Storm Water:H. Soil Gas3. HNO3. pH < 28. MeOHin the walk - in fridge (Room 190A) NO2N03 D. Surface water1. Air Canister4. HCl, pH < 29. Otherand notify appropriate staff for E. Wastewater5. NaS010. Other/ Containersnext day check - in. Ammo Sample (DDateTime Comp Grab MatrixSample Specific Comments VF01 05/21/2024 12:41 E 2 VF02 05/22/2024 08:25 E 3 VF03 05/22/2024 12:00 E 3 Relinquished by: (Signature)Received by: (Signature)DateTimeRemarks Ali, NafisahDigDatie:t 2a024l.0l5.y22 18s:5i7:2g3 n04'e00d ' by Ali, NafisahLaura Glowack (ESAT) 5/23/24 8:58 Relinquished by: (Signature)Received by: (Signature)DateTime CAFO samples collected at different farms. Relinquished by: (Signature)Received for Laboratory by: (Signature)DateTime Distribution: Original Accompanies Shipment. Copy to Coordinator Field Files:FSBFORM CHAINCUSTODY! Revision 1 05/6/2022 ED_019088A_00004946-00048 US EPA REGION 1 SAMPLE RECEIPT CHECKLIST PROJ #: 24050033Receipt date: 5/23/24 SURVEY NAME: Concentrated Animal Feeding OperationREC'D BY: LOCATION: Laura Glowacki (ESAT). Osc / Po: Nafisah AliSITE ID:SUPERFUND: N WERE SAMPLES SHIPPED?COMMENTS: TRACKING #: DATE / SENT:Wastewater samples NO. Hand Delivered COOLER TEMPERATURE UPON ARRIVAL 20 C / NA 9 STNL CHAIN OF CUSTODY PRESENT? 9 AMMO COMPLETE? Y 9 TP CUSTODY SEALS PRESENT ON COOLER?N SAMPLES? WERE SAMPLE CONTAINERS INTACT?Y 9 TSS 8 $ STA WAS SAMPLE PRESERVATION DOCUMENTED?YSamples relinquished to walk in COCSample Containeron 5/22/24 by Nafisah Ali and APPROPRIATE SAMPLES VOLUME recieved by Laura Glowacki on 5/23/24 FOR REQUESTED ANALYSIS?Y SAMPLES AND COC MATCH?Y IF ANY PROBLEMS WAS PROJECT MANAGER NOTIFIED? BY WHOM? Y APPROPRIATE SAMPLE CONTAINERS? SAMPLES WITHIN HOLDING TIMES? ALL ANALYSIS SPECIFIED ON COC7Y DATE / TIME OF COLLECTION ON COCY TURN - AROUND TIME: 4 weeks Page 8 of 8 ED_019088A_00004946-00049 End of Report ED_019088A_00004946-00050