Document q3QaO84vXm9bMDzDNZJ98M27E
i4l.-2fc-03T5
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE
Identify: Perfluorooctanesulfonyl fluoride; may also be referred to as POSF, or FX-8. (1-Octanesulfonyl fluoride,
1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluoro-, CAS #30735-7).
Remarks: The test substance is a clear liquid of uncharacterized purity. It is described as FX-8 (cc842-2) washed sample.
METHOD
Method: Protocol Reference EAR/WAS 5/9/84 Test type: Not noted. GLP: No Year completed: 1984 Analytical monitoring: Dissolved oxygen concentrations. Stock and test solution preparation: Test substance (0.309 g) weighed directly into BOD bottle, 50 mL deionized water was added, and the bottle mixed on a magnetic mixer for ~ 30-minutes. Bottle was capped during mixing. Inoculum source: Activated sludge mixed liquor from the Metro wastewater treatment plant - St. Paul/Minneapolis, MN. Inoculum holding: None - used the day it was obtained. Element basis: Respiration inhibition as determined by oxygen consumption.
Method remarks: After mixing in the BOD bottle, it was noted that the sample settled to the bottom, forming small droplets or beads. Some of the sample appeared to emulsify.
RESULTS
Nominal concentrations: 1000 mg/L test material solution.
Element value:
6.75-min IC50 = > 1000 mg/L
Control response: Adequate - 6.5% difference in respiration rate of the two controls.
CONCLUSIONS
The test material exhibited no inhibitory effect at 1000 mg/L over a 6.75minute exposure.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
006617
DATA QUALITY Reliability: Klimisch ranking 3. The method used was not agency approved. This study lacks sufficient documentation, characterization of the test substance purity, and analytical measurement of test concentrations. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number B1229. OTHER Last changed: 5/18/00
006618
3M Environmental Laboratory
Route Toxlcltu of a Subetance to Rctlvated Sludge htxed Liquor (SLUDSE"TOX) Protocol References EflR/WflS 5/09/84, Lab Request No. B1229
STOX.DRT
006619
3M Environmental Laboratory Acute Toxicity of a Compound (Substance) to.Actlvated Sludge Mixed Liquor
/ ^ i / ^ rtOC01 ReferenC6i EAR/WAS 5-09-84
Lab request no.:
Date
Analyst:'
The activated sludge mixed liquor was collected from the St. Paut/Minneapolis
Metro Wastewater Plant on
-f h c !&</
Sample d e s c r i p t i o n : C & ll
p y -? /
U A S t* i>
Preparation of stock solution:
grams of sample were dtluted to
^ w l t h deionized reagent water. Date of preparation;
ml
" 'T ime" " ' "
:ntration OF TEST MATERIAL,,IN ACTIVATEO SLUOGE MIXTURE
600yyu\tf A%'0iMimin. sec. c f t j f f /
?lnW>l
f
7-rr 7i(,o
'
1^0 7. n /
?./r/ ho
l.nZ n. 'T
2- (e\Hf
3 ^,/<o y/)o
t/,oo
yr )-sr
o 0
, l S S'.00
3. ?o
l*tcV
L.I'T Co<r 7.9o
72r" ,
Cp /3 / * * "
/. 3 0 .
/. V 7 j"
o . f r, 7
, 7 f. i'o j)- - 6 . 7 ^ /WCv - \
>
I % B ES T! OPYAII I't ll inMuCu
% 7f 1 T^
crnZ-U-4- = . f T7-
--
3MMENTS:
df
. y p 0 p -tTtCz. 30 ^ *
I'Vu-y tr>^ 4. fV^X<yr^/'-4r
u, r
JL ^ Mta-SXr *iL
.
r^
: o.? f
. s,
;"T v
b'O n-. L C'P ie ^c n ~ .^> V /
3 v' , ^c c^7*
jj \ A / ' t-M-jC/ /
+zrrz `^ T r .
4^
r v ,~ '7
3-27-84
ENVIRONMENTAL LABORATORY STATUS REPORT ALL RESULTS TENTATIVE
LAB REQUEST NO. B1229
REQUESTER NAME: NH/EAR )EPT: 0535 JROJECT NO! 9970012600 DATE RECEIVED: 2-21-84 DESC: FX-8 (CC 842-2)
CONTRACT LAB:
CONTRACT LAB COST:
0
3M E-L HOURS:
62
EST CMPLT DATE: 5-01-84
DATE COMPLETED:
3AMPLE NUMBER TWO HILL BE GENERATED FROM TWO EQUAL VOLUME HASHES OF SAMPLE DUMBER ONE.
SAMPLE
DATE
CODE
DESCRIPTION
TEST
RESULT
H 010
1
SHPL-PREP
COMPLETE
2
HASHED SAMPLE
96-HR-LC50
C-B0D-05
SEE REPORT
C-BOD-IO
C-BOD+20
SOLUBILITY
BIOSORP
JEG-SPEC
SLUDGE-TOX #
# - UPDATED DATA *= CONTRACT LAB
006621
4-11-94
ENVIRONMENTAL LABORATORY STATUS REPORT ALL RESULTS TENTATIVE
LAB REQUEST NO. B1229
REQUESTER NAME: AMW/EAR DEPT: 0535 PROJECT NO: 9970012500 DATE RECEIVED: 2-21-84 DESC: FX-9 (CC 942-2)
CONTRACT LA3:
c o n t r a c t LAB COST:
0
3M E-L HOURS:
47
EST CMPLT DATE: 5-01-84
DATE COMPLETED:
SAMPLE NUMBER TWO WILL BE GENERATED FROM TWO EQUAL VOLUME WASHES OF SAMPLE NUMBER ONE.
SAMPLE
DATE
CODE
DESCRIPTION
TEST
RESULT
1 FX-8
SMPL-PREP
COMPLETE
2
WASHED SAMPLE
96-HR-LC50
C-8 0D-05
SEE REPORT
C-BOD-IO
SEE REPORT
C-BOD-20
fSEE REPORT
SOLUBILITY
BIOSORP
SLUDGE-TOX
# = UPDATED DATA * = CONTRACT LAB
006622
