Document ppyVv8vzde2QV9558vVmmbEVE

AR126-03%3 ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: Perfluorodecanesulfonate, ammonium salt; mayalso be referred to as PFDS, FC-120,or 3M Sample J2905-1. (1-Decanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10heneicosafluoro-, ammonium salt, CAS # 67906-42-7) Remarks: Test sample is a mixture of the test substance in water and butyl cellosolve (approximately 25% test substance, 37.5%butyl cellosolve and 37.5% water). All values reported relate to this mixture. No calculations were madeto adjust for the actual concentrationofthe test substance in the test sample. Test sample was from 3Mproduction Lot 164. METHOD Method: OECD 203 Type: Static acute GLP: No Year completed: 1992 Species: Pimephales promelas Supplier:Aquatic Research Organisms, Hampton, New Hampshire Analytical monitoring: pH, dissolved oxygen and conductivity and temperature only. Test substance concentration was not measured. Exposure period: 96-hours Statistical methods: Probit analysis. Test fish age: Not noted other than "juvenile". Length and weight: Average length = 26.5 mm. Average weight = 0.14 g Loading: 0.09 g fish /L Pretreatment: None Test conditions: Dilution water: Aerated, filtered natural well water Dilution water chemistry hardness: (from a 160 representative mg/L as CaCO, sample): pH: 7.0 `Conductivity: 640 ymhos/cm Stock and test solution preparation: The test concentrations were prepared by transferring appropriate weights of FC-120 0a120 dbiarsecetdlyotnottohtealtseastmpclhea,mbneortso.nTpeesrtcecnotncceonntcreanttiroantsiowneroef tphreeptaesrted swuebrsetianniciaelliyn ctlheearteasntdsarmepmlaei.neAdlctleesatr vtehsrsoeulgshocuotnttahienisntgudFyC. -120 CStoanbclelinttyroaftitohnestedsotsichnegmriactae:lOsnolcuetions: Not noted sEoxlputoisonu,rweavteesrsedlesp:th2~01l8itecrmg.lass aquaria containing 15L of test NNuummbbeerrooff friesphlipcearterse:pltiwcoate: ten WaNtumebrecrheofmicsotnrcyendturraitnigontsh:efsitveudpyl:us a negative control Dissol6v.e8d-o8.x5ygmegn/Lra(ncognetr(o0l --ex9p6oshuorures)): pH ran5g7e -(08-69m6gh/oLur(s1)5 mg/L exposure) 77..008-.802 ((c1o5ntmrogl exepxopsousruer)e) RESULTS Test temperature: Mean temperature stated as 23 +2C ENloemmiennatl vcaolnucee:ntra2t4i-ohnosu:rBLkCscoon=tr>ol1,52.m5g,/4L.0, 6.0, 10, 15mg/L 4782--hhoouurr CLCaepo => 15 mglL = 11 (9.7 - 12.6) mglL 86-hour LCeo = 4.8 (4.0 -- 5.7)mglL. All element values based on nominal concentrations SPtraotbiisttaincaallyEsvisalwuaastiuosneodf Mortality: to calculate LCso values and the corresponding confidence limits. Cumulative percent mortality: TesmtaCto.ne. Tans boon] 0|%| 5 | 4| Le ToTw[os| wo] 000121 ACOOMNLCLLUUSSIoOnNSs 0000 T4.h8emtge/stLswaimthplae9956%-hcoounrfLiCdseoncfeorifnatetrhveaaldomfi4.n0n--ow5.w7amsgd/eLt.ermined to be. wTaestteirn(g 2w5as%csounbdjueccttemdatoerniatlh)e. "aTsh-esovlad"lumeisxtruerpeorotfetdhaepspulbyjetcotthmaattemriixatlurine and not directly to the subject material. Submitter: 3M Company, St. Paul, Minnesota, 55133 Environmental Laboratory, P.O. Box 33331, P0 DAAmTAAQ0 QuUAAL0 TITYY 0000000000 aRnealliyatbiiclailtyd:atKaiifmorisdcehterramnikniantgi2o.n oTfhtihsestteusdty,suwbhsitlaenwceellcocnocnednutcrtaetdi,onlaicnktshe tweasst stoelsutteido,nscaarnedmduestterbmeintaatkieonnwohfetnheinstaemrpprleetipnugritthye. rBeseuclatsu.seThaemisxotluvernet in the mixture toxicity of the could have enhanced or restricted fluorochemical component. biological uptake and/or REREFFEERRENCCEESS~~~ 0000000000 ITnhciosrspotruadtyewd,asHacmopntdounc,teNdebwy HEanmvpirsohSiyrsetoenmsbeDhiavlisfoiofntRhees3ouMrCcoemApnaalnyys.ts, o0 OmTHeE0 rR 00000000000 Last changed: 5/18/00 000122 | T2904-| | StaFtaitcheaAdcuMtienSnTtoowux,idcyPiTitynietpolhfeatFees-1p2r0omteoletshe authors RToibmeortthy1.3. Baoerrai Study completed Mazen 1952 Performing Laboratory ResouErnc`veoinreAonLssayifsyatayetensts,teDIinRvcoioasrdipoonrated Haspton, New Hampohire 03842 | Page 1 of 18 000123 1. sueany pLroormeTlh3eeMs,CoAmcpuiatsneydetfsoocrxriic9di6etdyhouroisfn EnviroSystens Division of dutPrhCii-ns1g20fFineabtlrouarretyphoar2t7f.attohTeMhaaedrctmhiensnt2,owv,a19s9P%ic.moemuipbehsaselenenes Resourc SHtaannpfeohridz,e. RoberItE BWooArt, coanndducTtiemdothbyy JeeanAnnaelyMsatgsa,ra, Ward. InPce.ter inK'owaHlaemppit,on.EllNeenw jCIaoecnmecpreeTnrhtearctoaultzlieoeoLScn0tso%efd ov2f3aatst:es2tHapCmes.prutfboosnTrt,haseneNcdeedwilaHunuatdnidoaenrdwialstutetaritoiwncasvectofenirdlitcteorineodtnrsonlatwuairttahsa mfeirvee ers il4,oo0pinMaPaloilnlt.caoinnc6e.0ndtirs0as9to/ilLov,nesd1o0o.xf0ygmenF/Cl-c,1o2n0acnednwmtepr1rsa5eht.:i0iroemn.as0lia.mAbeeo/rvNkaeotmia(ioncnnoaalncwtcacseroponrn)caoptl2ee.5mprmleoyvyiie,.d were used for all calculations. entoasioms u{nAdqeuraFtiiochtestReeesdecaorncdihintiOotrnhgseanitfseomrsst, `mvoHeraremepttpohunar,nchaNs1ee5wd xposure. the control fish had dfaHryaosmm:psahicroeAm)fmteearrncdia9l6accmsoluwiprmpsaltieeedfr lobfeygeirnaangpiepnrgoxwoieftmatwtheeali.yghsttu0d.(y0b.9lotGt/eLd. arya)nAoLafLve0r.af1ig4sehgt,woetrarelesiulnletngigontoghdoinfco2an6d.il5ceiaomsnimngaotnaceanreesn 236 42 DE5h.oxo7upurorsmu/rL`CeLaSn.Od ofoofnOen4fo.a8toohffmeagtd/thhLeemFiteCni-hng1r2hoe0twe,s swuirttvohiviatnhge95%tfeicssothnefsxiupdboessntecadencteiontr1eer5sv.ua0lltmeeodfrii4n.a0ta af7o2u,lmJhio0nu-gr0.s|mge/xLhNoiboaitttheedr3e6rshruobaultreistchaeslxwhieimfbmfiisentugcer.tdvsiavviTenhlrgeoesstfohiborssfheeereevqxseupudiorlsviieinvbdirtnnigueym1fal5na.dn0 mevaarsreeadt errecie during the 96 hour exposure. test seecss Page 2 of 14 000124 | II. nex op TAsLEs | ace Table 1. Cshaemmpilceaolfchnaartaurcatleriwezlaltiwoanteorf uaserdeparsesdeinltuattiiovne B water for toxicity test Table 2. Survival data from toxicity test 5 Table 3. Mteodxiiacnitylettehsatl concentrations (LCSO8) from 10 Table A.1. oCxoyngdeuncticvointcye,ntrpha,tiotnempmeeraastuurreed,duarnidngdistsoxoilcvietdy 1 test Page 4 of 14 000125 -- w. vRooucTION otbhjeectfiavTtehhiesadof omtitunhdneyow,wsatsaudyfsrpevosanhssuoarttoeedrdebftyisehr3.m%inCeTomhpetahnerye,paocruSttt.e cotPnaoutxlai,icnistMyinsnoeefcstoiFtoEan-.si20tThahtreo dOtfheesctrwahitebeeriqtnuhvaeelsittimygeattdhiaootdnas.caonldTlhaemcattreeedproiradtlusrianelgssoptlhcoeoynettdeasiitnn.stahne Astpupdeyn,diandthattheprreesmeinetss mest suBstaxce: Vv. xemHoDs i waTERINS EnvizosyFsCt1e2n0s bottle that woasn(EnNlvoaivbreeoslsblyeesrdtes1sw3,ith19S9a1mt.pheleItfMoulwalaobsweircnogntSa1ii0nn3ef)dormiawntaisaon2.5d0elailv~e3rp3el9da0sat-it1co FsCu-p1p2l0i,ed ume the Lobtteyst J1n64m,at`Ceor2mi-paBalunyt,owxays935EsttBhouarsnehodlA.veinnueF,tEh-e1St2.d0 aP(kaaulaL,iighMrtiononmbersotowetnmap.elriagtuPoirrdieys.ewatsao rfoerseraveminismaummpleof (1a0ppyreoaxrism.ately 1.3 grams) will be retained at pavireSyeteas DrLuTIon wzen: THaemspteihnigrMea.twears usceodllecftoerd Water was ascftcrolormiemdawteiilonlnsSo0f0a-tgtaelstlEonnovripgroaolnsyiyessttmhesyalsaenndeinfotraHnaaklm,lpthtooenxrieciNteiywt wvaatseraaercaotepdr.esenteRdesuilntsTaboflech1.emical analysis of a representative sample of est orcas: sThienygleweJrusovoeunrcipeluerchaanfsdaetdhweaedrferommiindaneonwtscifoimeeedmrpcliuoasyliendgsauspanplitaeepsrptropo(rrtihgeaatnAeiqsumtasatxiowcnecraReiecsferaKoremyc.ha oarcgcalniineamtsed fish vere Diatvisitohne weighed ofBatnvRiersthooeSuyrsccteoeAnmncsallufysasictoisnl,ityoIfncf.o,rthmeHoamrptetooxtnih,caintNye15wtdeHasaytzs.p.ahiPrCreoi)notrraosnlod tctoronetdaiitntegid,onsfofrisidhnisewaanesreealalnmdaigtnhlteaaysisnweedraqeuianfrrie1ue0s0.o%fdDaipulpruatirineognntacacstlieicrkmnaeutsnisdo,enrifnifjsluhorwieeCrshe,esasnnoodtn 2a142bn.o5dr9am,yaslitainpedrsiorthaettotdhtiehsesboetlgevisetndniinngoixtyiogfaetnitohneCottnehscete.ntteraaDptueirroianntgurwetahseraanmgcaecidlnitmfaaritonimeodn21p.e1a+rbioovtdeo 8TH.O03)BGo/nLc.e orFitswhiceverdeailfyodbeafocroemmetrhceisaelctf.ish food (EnviroSystems lot nummer . Page 5 of 14 C)0012 ww TOBle 1. nCahteumciaclalwelclhaaratcetreriuszeadtiaosn doiflutiaonrweaptreersenfotratiSvoevicimesympleestof P_ar--ameter --_-- wi HoUnaisturoefont|ReMpLoiermtieitng #0 unses - VVVeaalre uuezed i) Conductivity umhos/cn - sso Hacdnass ool as coco, 160 opezagtaincoicdheisorine wart os wo Oporngtaincoipdheossphorus ug/L os wo Pobliypchehnlyolrsinated ssn os wo H_ot--es: 21.. pNHD, =hancodtnedsest,ectaendd actonodructaibvoivteysvheererempeoarstuirnegdLiimnicd.ilution vcIaontltelrreocdtcuecodtlildoeuncrtoiefndgofrrrgooaumntiitnehesescbaioannntdnruopalelsttwieascttiedrveesaqnsudearliFvpeyeblodcaescsesb.ienLgo. Page 6 of 10 000125 11000ToorTerg. a"atceax'3e6Thouoeesrchaersaevtas.onsucevcbievneeeat 200 bn 000 maps 15 nolL 38 sat, 40 sore 55 nafs ans 105 ark. ne cone eas ropes race 31107 accoreins to pricebores ose ones (90eL- oe She COoncaIntiasionsea"rGE, SLhoet.o mcaasrt,saterial sere 0not. (coments 2.5 mah coapcehoxiCrnToaentetannlyiynelf2i0ah TCh2veere1xSenetwquiaclahlk,y 45ipesereebeoirbveneeedTeedngiinnaemsooangibtew3s5oopcaroopstbenicaostaennc)oif PS2Er0rEoIpif0rmoeontpneeiornriathctase"l"aylvLeaoits3nh6guisoucTnhht"arc'.asofe5i6seaaTTicoolhusytvceauLsanisstmesiel1st)sei"rncaeadsnet3r5oens.ihomonucbracvaoiTsoiringothoyeTnaivovheseeserceS5umhsghoiooeareeseidanrcm1myn3n prior errr SSsonottfoortinneiidnT"eedaevtrifnsecmucbattelsrliy(cs1h0oaf0saCiosck"uooccervosiicsvositnaigiinouinot,trigaaeionyrisamnseShheireecasvnhtid"eeeito2ha4ei.mbio0ocbnecgsu,ic3os0re,nhcaomevseer35os50f SSSCooiAnrsRdeauucEcriEtyvievcsihStahytno"exnTyioolnmeoaoenttasai"(rn5)e5a1FawTrHeisaneestsrsmmmo5ie7dnsentsuitrmeeeadrte)r'anphhHbL(-rseeScchmromradnsannaidreStoyebpeos1eht1ac5cancm:hesaSooonnt,e csLae6ta5hc0oida,t"iwTacoinnmuleeinntat"eC"couohrafneriecaauutntehtcseeuslLt(eoseMctaioLcmhinatSynye,lionuetiaBoBsoteiiiaom)nwsearlewoheneseionpvSshieesorrgpoarrseymtoeerss3s2,BsybeaynhoeaesteaDenoodcncanohransdse 00012 . --E--E ------------ ------ ------ -- ---------------------------------- vi. Resurs somainedAllcletaerst thvreosusgelhsoutcotnhetaitensitn.g BFiCo-l1o2g0icawlereandiwnaitteiralqlyualcilteayrdaatnad gApopneenrdaitxed A,byrestpheectiavceultye. tOonxeicihtunydretdestpercaernet control exposure. Control fish had an average psurrevsievnatledocicnurTraebdlei2n total length of 26.5 atnhde mm, 0tedst.an aLvoeardaigneg 0.09 g/1. wretateweidguhrting (btlhoettedtodxriyc)ityof 0t.e1s4t gwaast tahpeproondximoaftetlhye The pcornefsiednetnecde 24, 48, 72, in `Table 3. and T96heho9ur6 limits) is 4.8 mg/L LhCoSuOrs LfoSr0fviaslhueexp(oasneddastsoocPiCa-t1e2d0 FC-120 (4.0 - 5.7 mo/L). One of a95r%e the ithgrheto ssuurrvviivviinngg suimming. The fifsihshexpeoxspeodsedto t1o5.015m.g0/Lmg/aLt a7t2 4h8ouhrosuresxhiabnidteodneerorfattihce threa surviving fish exposed to 10.0 mg/L at 96 hours osxuhbilbetihtaeld eaffelcotsss owfereeqoubisleirbvreiduminaandny etersrtatviecsseslwimdmuirnign.g theN9o6othhoeurr exposure. Page 8 of 14 000129 Ttble 2. survival data from toxicity test _-- coNnocaenttnraaltion --_--(ng/L) ep. _-- Number Alive Number Affected Ohr 24he 48hr 72h 96hr r OhT24hrT 48he T 72he S6hc Ofeontrol) 21 1100 1100 11 110 110 00 00 oo oo oo 2.5 211010 110 110 1100 180 00 0o oo oo oo "0 2101010 11 11 110 105s 00 o0 oo oo oo 6.0 2i0101 1100 1100 110 03 00 00 oo oo oo 10.0 21 110 1100 110 5s 21 00 0o oo oo 12 15.0 211010 1100 88 21 0o 00 oo o1 o1 -- ---- Note: 9A6llhouarfsfeacltoeod efxihsihbietxehdibiateldosserroaftiecquislwiibmrmiiunmg. and affected fish at Page 3 of 14 000130 . | Tika 3. Basia Leth concnssions i500 from Sonic Sone i y -p_rromss wr coe ontaoTe cntone=ts mene wre som win sem -~ mare 26 sou nom saemsmn Corn oe sami mes mee eee eee eee | 3 i ! i Page 10 of 14 i 000131 VIL RereRences FCO: oi1i98,4. Aprile, YGautledosl.ines 1984. MfeotrhoTdasti20n3g, ofFCihshemiAccaultse.ToxSieccittiyonT2e:stEffmeacctmseoonn Stephan,U-S--B.EPA1.983D.uluCtohm,pumtye.r PPerrosgornaaml fCoormmtuhneicCaatlicounl.ation of LS values, i Page 11 of 14 000132 AependixA. WATER QUALITYDATA PROMTOXICITYTEST | | | || ` Page 12 of 14 000133 | Table A.1. cCoonndcuecnttirvaittiyo,n mphe,asutreemdpedruatruirneg,toaxndicidtiyssotlevsted oxygen --_-- concNeonattrnaatlion --_--(09/1) Rep. _co--nductivity (umho/ca) oO hr 2 hr 48 he 72 hr 96 he on hoe h2ee 4h8e h7e2 h9e (control) 21 664400 666400 665700 666500 663200 77..00 77..88 77..33 88..00 88..12 25 21 665500 667700 661200 666600 666700 77..00 77..99 77..33 88..00 88..22 a0 21 666600 667700 667700 666600 61700 77..00 77..59 77..33 77..95 88..12 6.0 21 666600 666600 66700 6es2o0 66700 77..00 77..99 77..33 77..93 88..11 10.0 12 666600 667700 667700 667700 666600 77..00 77..99 77..33 77..39 88..10 15.0 2167600 66700 667700 662100 600 77..00 77..95 77..33 77..88 88..00 --_---- i Page 13 of 14 000134 - wt | em conan, || -_-- Tr cNooWmmiEnamle we h--_me-- MwORmEwE -- seL-- m-- msE----meETnmeRiaE) ||| Clemeol 3Domm imie mimin n s ewsmeae srm0o1s sseas 1J : 25 . 12s 20;ena20s PDoomaaammsimnenmsinms 86 J 7.9 2.6 7.4 7.0 meeoonsmoem7sa0 wo PDoommsimmssmmoimmaanne meeorsmrsmosaes eo Bo DDoommimsmmaemmnmemeaormemrama cann ese D1 ommsmimmomnmeesemrueamnanaeoaonn --_-- ene34 10 000135