Document ppgB5r5ZzYp5MLaVnL2KgY0aE
MICROBICS MICROTOXS TOXICITY TEST
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, oras a component ofFC-206CE. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was 502. The testsample isFC206CE. Currentinformatioinndicatesitisa mixtureof0.92% PFOS, 15% diethyleneglycolbutylether,76.09% water,4% urea,1.28% Sultonefoamer, 1.55% sodium octylsulfate1,.05% polyoxyethylenemonooctylphenylether, 0.06% sodium laurylsulfatea,nd 0.05% tolytriazole.
The followingsummary applies toa mixture with incompletely characteilzedconcentrations of impurities.Data may not accurately reflectthe toxicityof the fluorochemical component of the testsample.
METHOD:
Method: MicrobicsMicrotox@ Procedure Test type: Staticacute GLP: No Year Completed: 1989 Species: Photobactefiumphosphoreum Analyticalmonitoring:pH and osmoticcondition. Exposure period: 30-minutes Statisticamlethods: EC5o valuescalculatebdy statisticlailnearegression program providedforMicrotox@ Test organism source: MicrobicsR'eagent. Test conditions:
Diluent:Microbics2% NaCl solution. Test temperature:Not noted. Stock and testsolutionpre.paration:A primarystocksolutonwas prepared inMilliporMeilli-Qwater. The pH ofthestocksolutiownas 6.7and an osmoticadjustmentwas made using200 mg NaCl dissolvedin10 mL stocksolution.Appearance oftestsolutionwsas noted as "apparently miscibleinwater,no colorapparent".Alltestsolutionswere made by proportionadlilubons. Exposure vessels: 4 mL cuveftes. Number of replicates:two Number of concentrations: Four plusa blankcontrol Element basis: Percentlightloss
RESULTS
Nominal concentrations: Blank control,125, 250, 500, and 1000 mg/L
Element value:
5-minuteEC5o = 650 (590- 710) mg/L 15-minuteEC50 = 450 (420 - 490) mg/L 30-minuteEC50 = 420 (390 - 440) mg/L
Element values based on nominalconcentrations.
Remarks: Tesbng was conducted on a mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The FC-206CE 30-minute EC50 forPhotobacteriumphosphoreum was determined tobe 420 mg/L witha 95% confidenceintervaolf390 to440 mg/L.
Submifter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,MN, 55133
DATA QUALITY
ReliabilityK:limischranking2. Testingmeets allcriterifaorqualitytesting.
However, sample pudtywas notproperlycharacterizedand ithestudylacks analyticaclonfirmationoftheamount offluorochemicalproportioninthe solution.
REFERENCES
This studywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number G1358-2, 1989.
OTHER
Last changed: 6/26/00.
ENVIRONMENTAL LABORATORY MICROBICS MICROTOXR SYSTEM
RAW DATA
LR it-IV P<%
TEST SUBSTANCE: TEST DATE ANALYST:
SAMPLE CUVETTE
Bl
ci
B2 C2 83 C3
&ac4
r
FINAL ASSAY CONCENTRATION Mia Itcl-3-
NITIAL7 READING 1(0)
FINAL KEAUINGS
5-MIN. 1(5)
15-MIN. 1(15)
30-MIN. 1(30)
BLANK CONTROL DUPLICATE
A 57
I@)uPII'Ca@e
77
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LR GlZ58-2 FC-2o6CE
111CRC)TOX(t-) DATA SHEEI-
5-MIN EXPOSURE
6/16/89 SAD
PAIR
1
7;
5
7 Fi
CONC.
125.OC)O 125. (:)00 2 5 0. C) 250. c--)Oo 5oO. CC)O 500. C)O(') %1 OOC). C)OI) 1,/.10000.00
BLANK Bo/Bt= 95 BLAN@*, RATIO= 0.8105
IQ/It
9().()6:/7,.0 96.()/69.() 1 OC). 59.(-.) 9E3.0/ 59.o 97.()/44. C) 1()1.0/47. C)
96.0/ 31.() 92.C)/ 29.0
77
G-OBS
0.158
(-.).:374 C).346 o.787
742 1.510 1.571
G-EST
o.151 ().151 ().333 o. 3:7. (-).735 c).77..5
1.621 1.621
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A7
All
EC 50
651,251
R =0.99557
SLOPE
593.503 Til 714.617
0.8691
INTERCEF,T
450
+6.4789
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RES.
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MICROTOX (t,) DATA SHEE'!'
15-MIN EXPOSURE
6/16/89 SAB
PAIR 1
4 5
COI\IC. rnA,)L
125. o(-)() 125.Ooo @:L50.OoC) 250. 04)1) 5i)()O.oi-) 5(')0O.(J(,.) % IOOC). oO(*) %10()()(.)C)()
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72
BLANi-%' RATIO= o.7579
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53o 1.113
2.3-9 2.339
Permr* Loss(13 A 19 .97
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EC Ojo
451.134
41%@i. 191 TO 490.189
R =().99476 SLOPE
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MICROI"OX(r) DAT@H SFIEET
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6/16/89 SAB
PAIR
CONC.
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125. C)o(.)
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69
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G-OBS
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547 7
1.150 67o
2.712
G-EST
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