Document ppR8GeaXm3G2a475BVKk2brxE

ACUTE TOXICITY TO FISM (MUMMICHOG) TEST S[JRqTAMr'P Identity:A mixturecontainingperfluorooctanesulfonawthei,ch may alsobe referredtoas PFOS, FC-95, oras a component ofFC-206 or L3243. (1-Octanesulfoniacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was not noted. The testsample isFC-206, identifiebdy the laboratorays "Sample C". Currentinformation indicatesitisa mixtureof0.67% PFOS, 17.5% diethyleneglycolbutylether, 78.91% water,1.33% Sultonefoamer, 1% sodium octylsulfate0,.04% sodium laurylsulfate0,.5% polyoxyethylenemonooctylphenylether,and 0.05% benzotriazole. The following summary appliesto a mixture with incompletely characterized concentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample. MET-ion. Method: Standard Methods (APHA) 1971 Edition Type: Acute static GLP: No Year completed: 1974 Species: Fundulus heteroclitus Supplier: FieldcollecteldocalliynMA. Analyticalmonitoring: Temperature,and dissolvedoxygen. Exposure period: 96-hours Statisticamlethods: TL50(mediantolerancelimitv)aluescalculateudsinga linearregressionequation. Test fishage: Juveniles. Length and weight: Average lenoth 35 mm Loading: Not given. Average wel@g'ht 1.1g (wet) Pretreatment:None Test conditions: Dilutionwater: Synthetiscea water Dilutionwater chemistry: pH Salinity 8.0 25 ppt Lighting:Not given. Stock and testsolutionpreparation:Not given. Concentrationsdosing rate:Once Stabilitoyf the testchemicalsolutions:Not noted Exposure vessels:5 gallonglassvesselscontainin1g5 L oftestsolution. Number ofreplicates1: Number offishperreplicate1:0 Number ofconcentrationsf:ivpelusa blankcontrol Water chemistryduringthestudy: Temperaturerange(0-96hours):20 1 *C Dissolvedoxygen range(0-96liours)4:.2- 8.6mg/L RESULTS Nominal concentrations: Blank control,1000, 1400,1800, 2400, and 3200 mg/L Element values: 96-hour TL50 = 1820 (1360 - 2420) mg/L 96-hour NOEL = 1400 mg/L Element values based on nominal concentrations. Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-206 96-hourLC50 formummichog was determined tobe 1820 mg/L witha 95% confidenceintervaolf1360 to2420 mg/L. The 96-hour no observed effectlevel(NOEL) was 1400 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilitKyl:imischranking2. Testingmeets the criterifaorqualitytesting. However, the sample puritywas notproperlycharacterizedand the study lacksanalyticaclonfirmationoftheamount offluorochemicalproportioninthe solution. REFERENCES Test was conducted by Bionomics,EG&G, Inc.,ofWareham, MA atthe requestofthe3M Company, St.Paul,MN, 1974. OTHER Last changed: 6/28/00 C,@ BIOASSAY REPORT SUBMITTED TO 3,M COMPANY SAINT PAUL, MINNESOTA ACUTE TOXICITY OF SAMPLE C TO MUMMICHTG (Fundulus heteroclitus), GRASS SHRIMP (Palaemonetes vulgaris), AND FIDDLER CRAB (Uca pugilator). Bionomics E G & G, Inc. 790 Main Street Wareham, Massachusetts May, 1974 METHODS AND MATERIALS These investigations.were performed at the aquatic toxicology laboratory of-Bionomics, E G & G, Inc., Wareham, Massachusetts. The susceptibility of mummichog (Fundulus heteroclitus), grass shrimp (Palaemonetes nLIZaris), and fiddler crab (,Ucapugilator) to Sample C, a brown liquid, tested as 100% active, was evaluated under static bioassay conditions for a 96 hour period. The results were reported as the median tolerance limit (TL 50)' the concentration of the sample in water causing 50 percent mortality after 24 and 96 hours. The predicted TL 50 value and its 95% confidence intervals were arrived at by converting the concentrations tested and the corresponding observed percent mortalities to logs and probits, respectively. These values were then used to calculate a linear regression equation. Test procedures for the static bioassays are those described for Fish Bioassay Procedures in the 1971 edition of-Standard Methods (APHA). -The mummichog, grass shrimp, and fiddler crabs used in these tests were collected locally by laboratory personnel. The mummichog had a mean weight and length of 1.1 g and 35 mm,respectively. The grass shrimp had a mean length of 30 mm. width of 20 mm. The fiddler crabs had a me Page two The test species were cibserved in the laboratory hatchery facility for at least 30 days prior to testing. These species were acclimated over a 10 day period,prior to testing,to laboratory test diluent water quality and temperature. During that period mortality in the test population was.43% and these animals were judged to be in excellent physical condition. The bioassays were conducted in 5 gallon glass vessels containing 15 1 of water at 200 c ( 1.0), and there was a single introduction of the sample into each test vessel. The test containers were not aerated. The test diluent consisted of synthetic sea water 1 with a salinity of 25 O/oo, a pH of 8,0 and a temperature 0+ of 20 C ( 1.0). Dissolved oxygen levels for the tests ranged from 8.6 at the beginning of the tests to 4.2 at the end of testing. Ten specimens were introduced into each test vessel 24 hours prior to the start of the assay. RESULTS The predicted TL 50 values and 95% confidence intervals are presented in Table 1. Table 2 presents a summary 1 Laroche, G. E. Eisler', and C. R. Tarzwell, 1970. Bioassay Procedures for Oil and Oil Dispersant Toxicity Evaluations. J. W. Poll. Cont. Fed. 42 (11): 1982-1989. Page three of observed mortality -for each individual concentration after 24 and 96 hours of exposure. The mortality syndrome in all tests'was similar. The mummichog and grass shrimp generally became dark and lethargic, lost equilibrium, and expired. The fiddler crabs became lethargic before expiring. SUBMITTED BY: PREPARED BY: APPROVED BY: Bionomics E G & G, Inc. 790 liain Street Wareham, Massachusetts May, 1974 Robert E. Bentley Aquatic Biologist Bevier Hasbrouck Sleight, III A Director, Freshwater Programs Table 1 Acute toxicity of Sample C to mummichog a (Fundulus heteroclitus), grass shrimpb (Palaemonetes vulgaris), and fiddler crabc (Uca pugilator). These data are based on results of bioassays conducted at the fish toxicology of Bionomics, E G & G, Inc., Wareham, Massachusetts. Species mummichog grass shrimp fiddler crab TL 50 - milligram/liter 24 hour 96 hour .,;@1,800<2,400 1,820(1,360-2,420) d No Effect Level (mg/1) 1,400 >11000.0 280 (230-342) 180 > 10,000 3,260(2,760-3,860) 2,400 a 0 Assays conducted at 20 C (t 1.0), mean weight of the mummichog 1.1 b Assays conducted at 200 C (+ 1.0), mean length of the grass shrimp 30 mm. CAssays conducted at 20 0 C (+ 1.0), mean carapace width'of the fiddle crabs 20 mm. d 95% confidence intervals. Table 2 Concentrations tested and corresponding observed percent.mortalities of mummichog (Fundulus heteroclitus), grass shrimp (Palaemonetes vul,,aris), and fiddler crab (Uca pugilator) after 24 and 96 hours of exposure to Sample C. Species mummichog grass shrimp Concentration (mg/1) 3,200 2,400 1,800 1,400 1,000 control 1,000 420 320 240 180 140 control % mortalitv observed 24-ho-ur 96 Fi6-ur- 100 100 100 100 0 30 0 0 0 0 0 0 0 100 0 100 0 100 0 40 0 20 0 0 0 0 Table 2 Continued Species fiddler crab Concentration (mg/1) 10,000 6,500 4,900 3,700 2,800 2,400 2,100 control % mortality observed 24 96 hour 100 100 0 100 0 100. 0 70 0 30 0 0 0 0 0 0