Document pmkJ79EqjvzadOxy9aE09B5Nw

NITRIFICATION INHIBITION TEST SUBSTANCE Identity:Mixturecontainingperfluorooctanesulfonatweh,ich may also be referredto as PFOS, FC-95, or as a component of FC-203CF-X or FC-203CF. (1-Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 501. The testsample isFC203CF-X. Currentinformationindicatesitisa mixtureof 1% PFOS, 20% diethyleneglycolmonobutyl ether,70.2% water,2.75% Acrylicfoamer, 3% sodium octylsulfate,1% triethanolmaine,2% sulfonatedalkylethersalts,and 0.05% tolyltriazole. The followingsummary applies to a mixture with incompletely characterized concentrations of impurities.Data may not accurately reflectinhibitionby the fluorochemical component of the testsample. METHOD Method: NitroseedTM -ST ToxicityTest Test type: Microbialinhibition GLP: No Year Completed: 1995 Analyticalmonitoring: Oxidationofammonia, pH and temperature. Statisticamlethods: Not given. Test organism source: PolybacCorporationB,ethlehem,PA. Received 8/2/95.NitroseedTm Lot G315. Test condition: Dilutionwater: MilliporMeilli_QTMwater Medium solutions: Per method. (pH 7-9 BiochemicalBufferand Ammonia solution1.0mL equivalentto 10.0 mg NH3-N/L). Test solutionpreparation: Test substance was added directltyotest vessels. Test vessels: 500 mL glass Erlenmeyer flaskswitha totaltestvolume of250 mL. Number of concentrations: fourand blankcontrol Temperature: 20.1 - 21.2 'C Total suspended solids and pH on day of testing: InitiTaSlS 1.153 g/L. Initialnd finalpH based on the blankcontrolwas 7.9 forboth time periods. Element Basis: Reduction innitrificataitonpH 7.8. RESULTS Nominal concentrations: Blank control,83, 116, 252, and 498 mg/L Elementvalues: 4-houErC5o= >498mg/L Element valuesbased on nominalconcentrations. Remarks: Testingwas conductedon ttiemixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicaplroportioanlone. CONCLUSIONS The testsubstance4-hourEC50 forNitroseeTdM -ST ToxicitTyestwas >498 mg/L,the highestconcentratiotnested.Thisconcentratiocnaused a 19% reductioninnitrificataifotnerfourhoursof exposure. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking2. Testingmeets allcdteriaforqualitytesting. However, the sample puritwyas notpropedy characterizedand thestudy lacksanalyticaclonfirmatioonftheamount offluorochemicaplroportioninthe solution. REFERENCES Thisstudywas conductedby the3M EnvironmentalLaboratory,St Paul,MN, Lab Request number N2977, 1995. OTHER Last changed: 6/28/00 sample Descriptlon er Environmental Lab - Work Sheet Lab Rgquest No. Page of Date: Analyst: L 4. .24 @k"i, .......... Nil ------------- .97, 7 L) 10 ENVIRONNE@ITAL LABORATORY 46e@NITRIFICATION INHIBITION TESTINO(ISO/DIS9509) RAW DATA TEST SUBSTANCE. LR A/-7'1'7 DATE A@IALYST @@4 P, Pg of READINGS IN TEST SOLUTION' ;pAT EXPa5URE TIMES - OXID.ATION OF ANNO@IIA TEST SUBSTANCE CONCENTRATION mg/L 0-H:tS NH3 Temp. mg/L pH or EXPOSIJRE TIME - HOIJF<S 2-HRS NH3 Temp. mg/L VH OC NH!, mg,,L 3-HR5 Terrp. PH OC 4-HR5 NH3 Temp, mg/L pH OC BLANK CONTROL Z 7,@l t, 7.1 @o,P, #V@.,R 7--1 7. '1 44, 114 7. ENVlRON4iE.VTAL LABORATORY MITROSEED R_ST TOXICITY TEST DATA REDUCTION LR /V ",,117 DATE sLttl3-,zA@JALYST PAGE.I o6 TEST SUBSTANCE- NH @ initiamtgll(l) 3 NH L32 Howtzmgll 3 A NH 3 @.1, HowLs mgll A kH 3 0 1 HouA mgll t LN 7.8 (2) T B&nk ConUct t, TEST SUITANCE CON,EN"TIRA.@T;I?O,AI'Z,MGIL 11 'S :@ ) I L, Iq 11 '[ 3zs I,@ i 11) ReSeA to AmmojUA P,%obe CatibiLation CL#Ave. (2) PeAcettt Lo.6.6oi t4itiiiication ( % LN ): I LN 7.8 T VH 3-N NH N 3- Hou)L (TeAt) HouA (ContAot) x 100 Whe.,Le: Sub,6c4.Zpt 7.8 de,6ignatu the pH o6 the teat. Sub,6c,tipt T de-6.ignate,6the time o6 expo.6uAe to the nUAi6ieu. q,-Iqrnev EC 195% Con6ideoice L@mit6) o-V 50 ENVIRONMENTAL LABORATORY N171ZOSEEDR_ST TOXICITY TEST DATA REDUCTION LR '77 DA.TE A.'4LAYS T PAGE 1 06 TEST SUBSTANCE: NH .3inaiat mglL(1) 3 NH3 @ tj Houu mgll 6 NH3 @14 Hou&6 mgll &H3 @ I Houx mgll 7.8 (2) LN T Btank conuot TEST SU TANCE CON ENMTIOHS, mgll p 3 1 /31) cl 77 /;.1 b (1) Re6ek to AmmopiiaPAobe CatiE@kationCuAve. (2) PeAcent Lo.6.6 06 Nittification % LN 7*8 S LNT _N!HH33_NV HOoUu&A (1CTOer6ttAt)ol I]x loo whe,te: Sub-6c,tipt1.8 deaignata the pH o6 the te,6t. Sub,6ekiptT de6ignate4the time od expo,6uAteo the nitki6ivL6. ECSO (95$ Con6.idenceLimit6) 1),.i, U,sing_