Document pmk2OQQOBVd1pw89Dz8X0X3pd

Monsanto MONSANTO RESEARCH CORPORATION / DAYTON LABORATORY / DAYTON. OHIO 45407 MRC - Dayton IDfPT /SECTION) Analysis type Of report REPORT REPORT NO.: JOB PROJECT NO.: DATE: PERIOD COVERED: TITLE: MDA-041 409.3010 25 February 1983 March 1978 - April 1979 PCB CONTENT OF MONKEY TISSUE authors: Beverly J. Warner x ABSTRACT: A subacute feeding study to determine the effects of polychlorinated biphenyls (?C3s) Cz on reproduction of adult Rhesus monkeys was carried out by Monsanto Industrial Chemicals u5 Company. Monsanto Research Corporation, Dayton Laboratory, carried out the analysis of various biological samples (fat, skin, milk, placenta, blood) for PCBs. This report documents the analytical techniques utilized and the data obtained on over seven hundred individual samples. \s \ 6 jj ^^ t^ 2Q Environmental Analytical Sciences Center Approval Date: 5- V--7 ? RESTRICTED DISTRIBUTION (see over) COMPANY CONFIDENTIAL Th> docomtm >s rt pfoparry el Monaante Aaaaareh Caporai.<jn and tha pa*0'" m iniw*h as ulMMpnf and diapotaon R eontamf CONFIDENTIAL INFORMATION <*a pa -mrmduead rwaatad *d"awth#ttad Oartoio o aam eutaida Monsanto Maaareh Corearattor* iNMiewp'* * without dp0*' authorialon HONS 000466 DISTRIBUTION COPY NUMBER -3 P. E. Berteau - A2SC 4 w. C. Hammann - T3A 5 0 . Hicks - T2F 6 R. G. Kaley - T3D 7 J . P. Kieure - T3D 8 J. C. Weber - B2SK 9 D. Wood - B2SD 10 D. J. Dahm 11 D. G. Glasgow 12 R. L. Maute 13 F. N. Hodgson 14 B. J. Warner 15 Central riles 16 Technical Reports Library St. Louis -- St. Louis St. Louis St. Louis St. Louis St. Louis St. Louis Dayton Davton Dayton Dayton Dayton Dayton St. Louis ABSTRACT ONLY R- K. Flrtcraft/Davto: H. L. wiliiams/Dayton D. B. Nelson/Dayton' MOTE: Distribution of this report is restricted. Requests for additional copies should be made through the principal contact at the Monsanto location involved or the Manager, Environmental Analytical Sciences Center, Dayton Laboratory, Dayton, Ohio. ACKNOWLEDGEMENT Contributions of the following KRC personnel to this project are gratefully acknowledgedt Analyses: G. E. Lowe A. J. Wright tOMiO COMPANY CONFIDENTIAL When no longer needed, or upon request, return this report to Central Files. Dayton. HONS 008*67 CONTENTS Section Page 1 INTRODUCTION................................................................................................................. I 2 ANALYSIS OF SAMPLES FOR PCB's ..................................................................... 2 2.1 Collection of Samples.....................................................................................2 2.2 Experimental Procedures................................................................................4 2.2.1 Summary of the Analytical Procedure ........................ 4 2.2.2 Modifications to the Procedure............................................. 6 2.2.2.1 Modifications Required for Baby Skin, Fat, and Muscle Samples........................................ 6 2.2.2.2 Modifications Required for Milk (Colo strum) and Blood SerumSamples .... 6 2. 2.2.3 Modifications Required._for Monkey. Chow Samples................................................................. 7 3 ANALYSIS AND RESULTS..................................................................................................... 3 3.1 Analysis by Gas Chromatography of the Extracted Tissue Samples........................................................................................................................ 3 3.2 Results Obtained in the Study................................................................. S 3.2.1 Adult Rhesus Monkey Fat Samples .................................... 8 3.2.2 Infant Rhesus Monkey Skin and Fat Samples ... 34 3.2.3 Liver Biopsy Samples....................................................................34 3.2.4 Miscellaneous Samples Analyzed on an Intermittent Basis.................... :............................................34 Appendices A. ANALYSIS OF CORN OIL FORAROCLOR 1254 ........................................... 4 3 B. ANALYSIS OF RALSTONPURINAMONKEY CHOW..................................................46 C. STATISTICAL ANALYSIS OF PCB CONTENT OF MONKEY TISSUE. . 47 iii MONSANTO A ESC ARCH CORPORATION . MOSS 008468 LIST OF FIGURES Number Face 1 Chrorr.atogran of 0.216 mg/ Aroclor 1254 standard solution............................................................................................................ 9 2 Chromatogram of 1.360 mg/ Aroclor 1254 standard solution............................................................................................................ 10 3 Chromatogram obtained from the analysis of a control dose adult fat tissue extract ...................................................... 4 Chromatogram obtained from the analysis cf a low dose adult fat tissue extract..................................................................... 12 5 Chromatogram obtained from the analysis of a medium dose adult fat tissue extract...................................................... 13 6 Chromatogram obtained from the analysis of a high dose adult fat tissue extract ............................................ iv monsamto "ESEAWCh co*oaat.o~ HONS 000469 LIST OF TABLES Number Pace 1 Sample Identifications Designatedfor Rhesus Monkeys Used in the Feeding Program.................................................. 3 2 Sample Designations for Infant Monkeys and PCS Dose Levels of Their Mothers................................................................................4 3 Gas Chromatographic Conditions ...................................................... 5 4 Cumulative PC3 Values for Adult Fat Samples on a Lipid Weight Basis ................................................................................................... IE 5 Cumulative PC3 Values for Adult Fat Samples on a Wet Weight Basis ................................................................................................... 19 6 PCS Content of Adult Fat Samples Received with Orig inal Shipment Shipped 27 March 1975 (Pre-Drug Samples) 20 7 FOB Content of Adult Fat Samples Received with Orig inal Shipment Shipped 27 March 1978 ............................................ 21 S PC3 Content of Adult Samples Processed by MIC and Chromatographed by MRC: Shipped 27 March 1978 .... 22 9 PCB Content of Adult Fat Samples Received with Orig inal Shipment Shipped 27 March 1978............................................ 23 10 PC3 Content of Adult Fat and Omentum Samples Received with Original Shipment Shipped 27 March 1978 .................... 24 11 PCB Content of Adult Fat and Omentum Samples Received with Original Shipment Shipped 27 March 1978 .................... 25 12 PCB Content of Adult Fat and Omentum Samples Received with Original Shipment Shipped 27 March 1978 .................... 26 13 PCB Content of Adult Fat and Omentum Samples Received with Original Shipment Shipped 27 March 1978 .................... 27 14 PC3 Content of Adult Fat and Omentum Samoles Shicped 4 May 1978 ...................................................................... '...............................29 15 Content of Acult Fat Samples Shipped 15 June 1978. . . 29 16 PCB Content of Adult Fat Samples Shicoed 24 August 1978 ....................................................................................................................... 30 v MONSANTO RESEARCH CORRORATION HONS 008470 LIST OF TABLES (continued) 17 PC3 Content of Adult Skin, Omentum, and Fat Samples Shipped 24 August 1973 ......................................................................... 31 18 PCS Content of Adult Fat and Omentum Samples Shipped 1 November 1978......................... . , . . ; --........................ . 32 19 PCS Content of Adult Fat Samples Shipped 6 February 1979 ...................................................................................................................... 33 20 PCS Content of Adult Placenta and Saby Skm and Fat Samples Shipped 4 Kay 197 8 ................................................................ 3 5 21 PCS Content of Baby Skin and Fat Samoles Shiooed 23 Kay 1978............................................................................... "..... 35 22 PCS Content of 3aby Skin Samples Shipped 15 June 1978. 35 23 PCB Content of Baby Skin and Fat Samples Shicped 24 August 1978 ..................................................................... '.........................35 24 PCB Content of Baby Skin and Fat Samples Shicped 24 August 1978 ....................................................................`.........................37 25 PCB Content of Adult Skin and Fat Samples and 3abv Skin and Fat Samples Shipped 1 November 1978 .................... 38 26 PCB Content of Baby Skin and Fat Samples Shipped 6 February 1979 .............................................................................................. 38 27 PCB Content of Adult Liver Samples Shipoed 7 .March 1979 ....................................................................................................................... 39 28 PCB Content of Baby Liver Samples Shiooed 7 March 1979 ...........................................................................V......................................40 29 PCB Content of Baby and Adult Fat and Muscle Tissue of Terminal Animals Shipped 18 April 1978.............................. 40 30 PCB Content of Milk Samples Shipped 2 May and 23 May 1978 ....................................................................................................................... 41 31 PCB Content of Milk and Blood Serum Samples Shipped 7 March 1979 ....................................................................................................... 41 32 PCB Content of Flacenta Samples Shipped 23 May 1978. . 42 vi MONSANTO NESEANCM COeeONATtON HONS 008471 1. INTRODUCTION This study is designed to determine the effects of polychlorinated biphenyl (PCS) compounds on the reproductive abilities of adult Rhesus monkeys and to determine effects on infants. In the course of that study, analyses were conducted for PCS residues in a series of materials. An existing procedure for detecting PCBs in animal lipids [Kaley, et al. , Bull. Env. Cont. & Tox., 115(6)1976] provided the basic methodology for PCB tissue analyses in this study. However, the following must be considered in reviewing and interpreting the reported tissue PCB concentrations. Although the original methodology had been used extensively for tissue analysis, it can not be considered fully validated for the present application. It was necessary tc modify extraction procedures for the variety of monkey tissues involved and the small sample sizes precluded sta tistical evaluation of the accuracy (recovery) and precision of the PCB determinations in the various matrixes- Thus, it is extremely difficult to determine whether variations in PCB tissue concentrations for an individual animal, or between different animals, are significant differences or the result of analytical variability. The limited size of the samples introdices its own difficulties. On a relative basis, it tends to increase analyti cal variability, particularly as the concentration of the material sought decreases. Small sample sizes also increase the probabil ity of inhomogeneity, which contributes more variability. 1 HONS 008472 2 . ANALYSIS OF SAMPLES FOR FOB'S 2.1 COLLECTION Or SAMPLES Forty-eight adult Rhesus monkeys were involved in a feeding study m which they were fed Aroclor 1254 (a PCS compound composed of 12 carbon atoms and 54% chlorine per molecule) for a period of one year. Fat biopsies were analyzed for PCB content approximately every two months during the study, which included both the oneyear feeding program, and an eight-month relaxation period. The initial samples in the study, approximately one hundred adult fat samples, were analyzed by the Monsanto Industrial Chemicals (MIC) Company in St. Louis, Missouri. All subsequent analyses were performed at Monsanto Research Corporation (MRC), Dayton Labora tory. The feeding studies, including excision of tissue samples, were carried out by Litton Bionetics, Kensington, Maryland. The animals were divided into four groups consisting of twelve monkeys each (eight females and four males) for the purpose of this study. One group of animals was used as a control group, and received no PCB's. The remaining three groups were fed low, medium, and high dosages of PCB (5 pg PCB per kg of body weight per day, 25 pg/kg per day, and 100 pg/kg per day, respectively). The animal sample identifications for the individual animals within each group are presented in Table 1. The PCB's admin istered to the animals were fed as a dietary supplement. Corn oil spiked with Aroclor 1254 was mixed with applesauce and then fed to the monkeys in addition to their normal daily diet of mon key chow. MIC has analyzed portions of each, individual batch of corn oil used; this data is presented in Table A-l in Appendix A. A representative sample of the monkey chow fed as a regular dietary staple was also analyzed for any background PCB levels present. These data are presented in Table B-i in Appendix B. 2 HONS 008473 TABLE X. SAMPLE IDENTIFICATIONS DESIGNATED FOR RHESUS MONKEYS USED IN THE FEEDING PROGRAM Control Group 7 64 -E 618-1 604-1 578-H 27 4 -F 569-H 200-F 67-F 227-G 739-F 426-G 4 18-J Female Female Female Female Female Female Female Female Male Male Kale Male Low Group 309-1 483-F 646-E 701-E 839-H 284-1 605-F 624-1 516-G 119-H 432-J 428-J Female Female F emale Female F ema1e Female Female Female Male Male Male Male Medium Grout 27 3-1 5s C-K 8 4 1-H 2S1-G 7 63-E 307-1 757-1 762-E 599-1 20-1 46-1 595-1 Female Female Female Female Female Female Female Female Kale Male Male Male Hich Grouo 152-F 486-H 226-F 269-1 312-1 287-1 770-1 338-1 706-E 425-J 427-J 600-1 Female Female Female Female Female Female Female Female Male Male Kale Male In addition to the adult fat samples analyzed for PC3 content, numerous infant Rhesus monkeys born to the female adults were biopsied and analyzed for PCB's they may have accumulated both during gestation, and after birth as a result of nursing. These baby monkeys (enumerated in Table 2) were biopsied on an inter mittent basis, and in most cases, skin samples were analyzed rather than fat tissue. 3 MONSANTO RESEARCH CORPORATION HONS 008474 TABLE 2. SAMPLE DESIGNATIONS FOR INFANT MONKEYS AND PCB DOSE LEVELS OF THEIR MOTHERS Animal Designation Mother Dose Level B-8483 B-8518 B-8532 B-8533 3-8557 B-8574 B-8621 B-8527 3-8562 B-8604 B-8754 3-8815 3-6 511 B-8546 B-8559 3-8560 B-3592 3-3603 B-8860 3-8542 Control Control Control Control Control Control Control Low Low Low _ Low Low Medium Medium Medium Medium Medium Medium Medium High Throughout the program, various types of additional samples were analyzed on an "as provided" basis. Several milk (colostrum.) samples taken from nursing mothers, placental samples, and blood serum samples were submitted on an irregular basis. Many of these samples required special processing techniques due to small sample sizes and/or matrix effects. All modifications developed to the established procedure are outlined in the Experimental Procedures section. 2.2 EXPERIMENTAL PROCEDURES 2.2.1 Summary of the Analytical Procedure The procedure used to analyze monkey tissue is taken primarily from the article entitled "Tissue Residues from Subacute Oral Feeding of Polychlorinated Biphenyl Dielectric Fluids," by Xaley, 4 MONSANTO RESEARCH CORPORATION HONS 008475 et al, appearing in the Bulletin of Environmental Contamination and Toxicology# Vol. 15, No. 6, 1976. Briefly, the method is as follows: the entire sample (the sample size for an adult fat sample averages 0.5 to 2 grams instead of 10-gram sample size suggested in the procedure) received is accurately weighed and homogenized three times with three 25-ml portions of hexane. The combined supernatants and washings are filtered through a bed of anhydrous sodium sulfate (that has previously been dried at 400"C) , and diluted to 100 mt in a volumetric flask. A 25 ml aliquot of the solution is evaporated to determine the lipid weight of the sample. A separate 25 mt portion of the sample is pipetted onto an adsorption column prepared by placing a plug of glass wool (previously dried at 400*0 in a chromatography column followed by a 13-20 cm bed of alumina (dried at 400*C and then deadivaoed to 54 with distilled water) and then a 2-3 cm bed of anhydrous sodium sulfate. The PCS's are eluted with approxi mately 152 ml of pesticide grade hexane. The eluate is trans ferred to a 5C0 mi Kuderna-Da.-iish evaporative concentrator fitted with a three-ball Snyder column and reduced on a steam bath to approximately 5 mi. The resulting concentrate is analyzed by gas chromatography utilizing an electron capture detector. The gas chromatographic conditions are listed in Table 3. TABLE 3. GAS CHROMATOGRAPHIC CONDITIONS Instrument: Column: Injection Port Temp: Detector Temperature: Column Temperature: Carrier Gas: Carrier Gas Flow Rate: Varian Model 3700 Gas Chromatograph with Ni-63 Electron Capture Detector 2 m x 1/4 in. glass column packed with 4% XE-60 on 80/100 mesh Chromosorb W-HP 220*C 350*C 200C isothermal High Purity Nitrogen 40 mi/rain 5 MONSANTO HeSEAKCH COB3AT I ON HONS 008476 2.2.2 Modifications to the Procedure The procedure, as written, can be easily applied to the analysis of adult fat, skin, omentum, liver, and placento, and to baby liver samples due to the relatively large sample sizes supplied. However, several modifications to the procedure are necessary to accommodate the reduced sample sizes of the baby monkey skin fat, and muscle samples, and the special handling problems encountered with the minute sample sizes of the milk and blood serum samples. In addition, the project requires the development of an analysis scheme for the determination of PCB in the monkey chow samples. 2.2.1.1 Modifications Recujred for Baby Skin, Fat, and Muscle Samples -- Baby monkey samples submitted for analysis are analyzed following the procedure by Kaley, et al, with volumes reduced when possible due to the smaller sample sizes received. The entire sample received is homogenized three times with separate 5 ml portions of hexane. Mo attempt is made to remove hair or other extraneous matter from the skin samples. The combined supernatants and washings are filtered through sodium sulfate and diluted to 25 ml in a volumetric flask. A 10 ml portion of the resulting solution is evaporated to determine the lipid weight of the sample. A separate 10 ml portion is placed through an adsorption column composed of approximately 7 cm of alumina and 2 cm of anhydrous sodium sulfate. The PCB's are eluted with about 100 ml of hexane. The column eluate is collected and placed in a Kuderna-Danish evaporative concentrator and reduced to a volume of approximately 3-5 ml. The resulting concentrate is then analyzed by gas chromatography. __ _____ . 2.2.2.2 Modifications Required for Milk (Colostrum) and Blood Serum Samples-- Due to the minute milk and blood serum samples received, micro techniques for PCB analysis are required. A 100 ul syringe is 6 MONSANTO RESEARCH CORPORATION HONS 008477 accurately weighed containing approximately 100 ul of monkey milk or blood serum. The sample is then discharged to the top of an adsorption column prepared by placing a small glass wool plug in a disposable, graduated, 10 mi pyrex pipette cut to approximately 150 mm in length, followed by 60 cm of alumina and 20 cm of anhydrous sodium sulfate. The syringe is weighed again to determine the weight of sample by difference. The sample is washed through the column by 7 mi of hexane and collected in a vial. The hexane eluate is then placed under a gentle stream of purified dry nitrogen and reduced to approximately 100 ul. The resulting solution is analyzed by gas chromatography. 2.2.2.3 Modifications Required for Monkey Chow Samples-- A special handling scheme was devised for the analysis of the monkey chow pellets. Approximately 50 grams (10-15 pellets) of monkey chow is finely ground with a mortar and pestle. The mon key chow is thoroughly mixed, and a 10-gram sample is accurately weighed and placed in a 33 cm x 94 mm single thickness cellulose extraction thimble inside a medium sized (85 ml capacity of the tube to the top of the siphon) Soxhlet extraction apparatus. Two hundred ml of hexane is added to a 300-ml round-bottomed flask which is fitted to the Soxhlet extractor. The solution is allowed to reflux for 12 hours, and is then cooled to room tem perature. The resulting solution is reduced to approximately 10 ml in a Kuderna-Danish evaporative concentrator. This con centrate is then eluted through the alumina/sodium sulfate chromatography column system with 200 ml of hexane. The hexane eluate is then again reduced in volume in a Kuderna-Danish con centrator to approximately 5 ml. The resulting concentrate is subsequently analyzed by gas chromatography. 7 MONSANTO RESEARCH CORPORATION HONS 008478 3. ANALYSIS AND RESULTS 3. 1 ANALYSIS BY GAS CHROMATOGRAPHY OF THE EXTRACTED TISSUE SAMPLES All sample extracts are analyzed by electron capture gas chroma tography utilizing the Varian Model 3700 GC with a Ni-63 detector. Peak area integration is accomplished by the Hewlett-Packard Model 3350 Laboratory Automation Computer Data System. Aroclcr 1254 standards are prepared monthly, and five to seven standards are analyzed daily. From these standards (Figures 1 ar.d 2 give two representative standard chromatograms), a daily calibration curve is prepared. All sample extracts analyzed are compared to the daily curves. Figures 3 through 6 are typical chromatograms obtained for a control, low, medium, and high dose adult animal fat tissue extract, respectively. For purposes cf quantification, only eight characteristic Aroclcr 1254 peaks are used. These eight peaks, which have been marked in Figures 1 and 2, were chosen because they give good linearity over the working concentration range, and little, if any, inter ference was found in this retention time window. Interferences do occur earlier in the chromatograms of the monkey tissue sam ples. These interferences most likely result from DC active substances found in tissue that are not removed by the alumina column clean-up procedure. 3.2 RESULTS OBTAINED It! THE STUDY 3.2.1 Adult Rhesus Monkey Fat Samples Due to the extremely large number of adult Rhesus monkey fat samples analyzed, a large volume of data has been generated. In order to present the data in a manageable fashion, two tables compiling the final PCB values for the entire project are included. These tables include the PCB values obtained by bet.-. 8 MONSANTO eeSEANCH CONOORATION HONS 008479 Figure 1. Chromatogram of 0.216 mg/. Aroclor 1254 standard solution. 9 MONSANTO RESEARCH CORPORATION HONS 008480 Figure 2. Chromatogram of 1.360 nc/1 Aroclor 12 54 standard solution_____ 10 Monsanto eseCH co*oe HONS 008481 =5}R- Figure 3. Chromatogram obtained from the analysis of a control dose adult fat tissue extract. 11 MONSANTO RESEARCH CORORATiQN MONS 008482 Figure 4. Chromatogram obtained from the analysis of a low dose adult fat tissue extract. 12 MONSANTO RESEARCH COBBOAATiOn HONS 008483 Figure 5. Chromatogram obtained from the analysis of a medium dose adult fat tissue extract. 13 MONSANTO RESEARCH CORPORATION HONS 008484 Figure 6. Chromatogram obtained from the analysis of a high dose adult fat tissue extract. 14 HONS 008485 MONSANTO AESEANCM COORA-ION MIC and by MRC. Table 4 tabulates the PCB values found in the monkey fat tissue samples on a lipid weight basis. Table 5 lists those values on a wet weight basis. The values presented in both tables are in terms of ug of PCS per g of weight (lipid or wet). For a more thorough review of the data generated during this project. Tables 6 through 19 can be found to give complete, data for each individual sampling date. Data entries of each sample included in these tables are sample identification and type, the date the sample was shipped, the date it was excised from the animal, the dose level (control, low, medium, or high), the num ber of r.g of PCS in the sample analyzed, the wet weight of the sample, the lipid weight, the percent of the total weight repre sented by the lipid weight, and the ug/g of PCB on both a wet weight and lipid weight basis. Sampling dates in the study include a predrug sample (Table 6), samples taken during the feeding portion of the study (Tables 7 through 13), and those samples taken after the oral administra tion of PCS had ceased (Tables 14 through 19). Feeding dates for the adult animals commenced on 4 April 1977 for all animals except 483-F, for which the first day of drug administration was 19 April 1977. Feeding of PCB's to the animals continued through April 1978. The animals were then monitored through December 1978 to examine the rate at which PCB's are expelled from the tissue. The following key can be used to identify sample types in all subsequent tables in this report. B - Baby Skin Sample Bf Baby Fat Sample B1 Baby Liver Sample Bm " Baby Muscle Sample C " Monkey Chow Sample f Female F * Fat Sample (Adult Animal) L = Liver Sample (Adult Animal) m Male M Milk (Colostrum) Sample 0 Omentum Sample (Adult Animal) P - Placenta Sample S Skin Sample (Adult Animal) Se = Blood Serum Sample 15 MONSANTO NESEANCh CONDONATION HONS 008486 TABLE A CUMULATIVE PCtl VALUES I OR AOULT I AT 5TAMEEK on A /.HMD WEIGHT BASIS (iqAj) IkKf Plf'Dltll i.o.L*vrii/))4/?lljn _ h/n__________________4/u H/> _ i/n _ _ i/i" _ *./mi,/; / m________ u^jn 7 I ni .U1K. 1M-C I fofitiol 0.44 0.44 0.71 I.O* tm-i t com mi a. it 0.46 4.42 0.24 1.04-1 f Coiitml Mil'll I Cunirnl I (Ml 1 . 44 0.14 0.14 1.44 2.14 0.44 I.UU 7)4-f f Comrol 0.44 I . II 1. 2D 0. 21 oc Hl-H ( Cool i'll 0.12 O.bl 0. 10 O. 10 joo-r r commi 0.4* 0.)b 0.1b 0.20 l-r I Cnnliol J27-C Coiilcol 0. 24 loot* but!4 0. II 0.14 0.W 0. II 11.2) 714-f m Contml 0.41 0.40 l.lil I. II 0 Oli>C Coni ml 14.0 0.12 2.12 7.41 B 4IA-J Conliol n. 74 0.40 1.2b 0.111 C*l m 104* 1 r Urn H> 4A)-r r Uow 0.44 0.47 0.11 l-bl 2. (4 4.14 0.1) 0.47* nB 1 01 *4b-e t um 701 -IS t Ur* 14-M I Low 0.14 7)1 0.74 0.22 12* 0.4 1.14 I .A4 0.1.1 0.1) 0. II .b r> 204-1 f low 2.72 4.11 1.1) I4.lt* o a o o iOW I 424-1 I llb-G Low low low 0.14 I . 10 0.1) 0.40 0.1) 2.44 1.A4 1.14 I.A) O.'ll n. 12 0.74 a 114-H m low 1.12 0.02 2.44 2.4) 417-J m low 1.b 7 4.74 J.10 0.4| Q 4 70- J * low n.m I.i9 1. |4 II. 12 0.14 0. 74 0.4* 0.14 0.21 A.74 0. I I 0.11 0.20 0.4H 0.24 1. 14* I.M l.'ll 1.40 ). )b 1.7b 4.AS 1.4? |.bS 1.11 ).4| |b) I. 1.4 VU II. 20 0. 10 O.S4 7.n <) 42 II. 7b n. 14 O.B0 4.IH I. I I. II I - lb I. Ill 1.40 7.70 1.14 2.9b 1.41 M.H9 III. I S. 17 7. If* II 7H U. *>2 11.71 0 , 2 O il. ll IS 0.1. (1.41 11 11 i>.bi Id.2* l.ll 2.91 II.HI* 2.1) 7.`)b 2 . b) b.4 I 7.44 7. II I 1.0 2(1.2 I M 10. II * * * * * 0 JO * 0.2)7 0.20 0.1.7 2.41* 1.1.4* 2.7b | J.ll* 2.)) * 114 S.b2 * * IH I A. 7) A. 01* 10 1 0.lb 0 O0 0 110 1 III 0.1) O.Ob * 0.07 0.44 2. lb 0.49 7.27 * 2.A! 0 2.b0 I II 7.94 7.04 7.1) 141 0.1; 0.IIH 0 411 0.41 0. It 0.72 0.21 0.41 0.)I 0.71 0.41 2.2) ).l) |b * 1.4 I 1M 1.1 A 1.14 1.b) 1 . 74 1.42 I .00 0.'ib 1. . I I (2. 40* 0.1 I b.ai 0.)) 2. ib A. 40 0.40 4.11 1.2* s.i; 7.1 ).i: 4.2' 4.0 2.91 I .01 5. II 4 . II 4 01 0.bl 0. )0 0.11 1. IA 0.41 0. 14 0.1) 047 0.04 b).2 0.1b 2.90 1.44 2.4) 2.04 2.10 4.41 1.4) 1.bO 111 4.08 7.17 ). 14 M|.||>|d wrt>|M ilpl'ilnUnnii lull Ii>i ('* n.<|.|c. tHti M*|<U Ifff l*nl. 4A)l*lymtl l.p til . tTctnlnbl miMl . 4w|ilei) only on w*t w*l<|l(t lao'ln l>V Ml) c T t/> o CD TABI.n A (rout i nurd) iC 'X V to O tt0 3 H 3 w *a $ 3 W Iwplt Ohm 1.0.l**ml)/?l M-Drui 5/17 1/71 9/71 4/17 \l/1i1/10 J/70 s/1*4/70 flMl 9/ i* n/u n/9 1U-J f IfrdluB 0.71 4.1* 12.1 l<nat 4* !V4 10.9 11.9 * 12 4 11.4 14.7 119 540-H ( HhIIm I.14 1.02 14.0 lnat 11.9 71.0 70.J * 79.1 70.4 44.7 n.o 41-N r rtrriiun Mt ).5* 19.f Ln*t 4* 42.2* 71.) 19.1 * 41.4 J9.2 40.4 * s 2II-C f Mil* 0.49 UlHl 10.4 Lnat14.4 11.4 71.) 14.9 14.0 71.1 * 10) o 741-C ( ItediiM )IM f 0.7| 0. 41 2.99 0.49 0.70 Ut44 4.40 ImIM 0.44 4.79 0.? 7 10.0 17.0 11.4 * 14.1 10.) 10-7 74.4 19.1 71.0 24.7 11.4 10.4 >z -i HH t Hl'l t S99-1 m Nrilltfi Mlia Pb'diyai J.09 0.14 1.74 4.24 1.41 4.J4 0.49 4.2 J 10.4 ImIM lM( 4 21.4 12.7 7.27 4). 10.7 )0.1 20.(1 )1.2 J1.4 * 4 24.0 44.9 41.1 )4.1 70.) 4.07* 20.1 41.4* 141* 11.4 M.9 9.99 10.1 o 10-I a N*<ll<* 0.49 1.04 4.44 11.4 10.2 71.4 >9.4 71.9 Jl.4 42.1 12.) 44-! a nrdtuB 0.14 0.42 4.22 4.14 4.04 10.4 10.4 11.1 9.44 9.44 4.99 595-1 b HrdtuB 1.20 7.20 14.) 0.74 41.0 71.1 94.4 97.9 11.4 41.9 >9.1 l2-r f 494-0 ( 224-F t 249-1 1 112-1 I 207-1 f 770-f t no-1 ( 709-C b 424-J a 477-J 400-1 * lllfh Hlqii NlqO Milk Ml* Hloti 0190 nidi Nlqte Hlqh HI9I1 Oil* 0.44 1.72 1.4) 0. ) 0.24 0.94 9.44 0.94 0.44 2.14 1.44 0.44 I i* I 140 4 45 ** ** 20.4 7# >0.4 24.2 ( 7# 12.0 74.94 47.2* 17.44 40.0 21.4 01.0 25.54 00.0 41.04 40.04 1) .0 74. ) 170.44 49.1 24.44 14.14 10.) 42.9* 41.9 24.0 boat 17.44 22.94 24.4 474* 51.0 41 0 244* 70.4 94.0 192 91.9 144 74.4 41.4 119 444f 144* 51.1 149* Jfl.O 90.4 297 49.5 141 14.0 111* 100 * 7)47 70.4 114 7 190 4741 14) no* DO* 97.1 114 42.7 144 71.7 144 144 147 97.4 50.4 144 99.) 59.4 79.1 44.9 9.57 72) )0.7 14.) 77.) 299* 70.) 1)1 97.0* 1.401 * 49.9* 29.) 701 >44 59.) 1.2 50.7 9 7 29.) >0.7 79.? 7#Mp(<4 7Bn taaplf datMaliiaUoM lut for tl**ao aoBjitra. t4aai|iil Of MIC. ObpMub itaflai. iTaiatnil inlaol. g OtfWttrd 0*If on a - *tol|ht tooolo b OIC. SC + ftft, TAIH.i: 5. CUMUI.AT I Vr fCll V A LIU 'S I'Oll AIHII.T TAT rAMIM.I'.S ON A WLT WI'.IOIIT BASIS .Mo n s a n t o r e s e a r c h C o r p o r a t i o n splc 1.0. Dose Level Pre-Oruq V77 m-c i iii-i r *04-i r 17 - II f 77-r r )t-H r 200-r t 7-r f 221-G U9-f m 4 24-g * 4 10-J m Cont rol Control Control Control Conirol Control Control Control Control Control Control Cunt rol 0*4 0.47 0.70 0.59 Loot** 0. 74 1.21 309-1 f 4ij-r r 444-C r 101-i r 39-H t 2#4-i r 405-r t *24-1 1 Low Low Low Low IaOW 119-11 42t-J m Low 0.35 0.44 0.11 0.51 0.10 1.14 0. 37 0.41 0.51 1.17 0. 70 0. 20 5/77 0. 54 0 44 0.54 0.57 0.04 0. 49 0. 24 Lout 0. 12 0.13 0.23 0. 34 0.42 0.10 0. 31 0.4 3 0.59 0.95 7/77 0.51 3. * 0.01 2.7) 0.9) 0.40 0.44 0.4) 0.47 0.75 0.74 0.92 1.41 1.17 0.57 1.30 1.24 0.43 1/17 2.02 0.01 0.11 0.14 0.22 0.24 0.13 0. 30* 0. 10 0.24 2.90 4.42* 0.74 0. 21 0. 20 2.39 0.27 0.09 4/77 11/71 I.Oft( (.ONt I.O'll UlMl * 1.0191 I.OHt 4 0.4* 1 . 14 1.0* 1. M 0. 1 I 0. 20 0. 11 0. 11 0.24 0. 29 1.14 7.3*4 1.0* 4.2* l.0 5. 2** 2.3* 2.2* 5.1* 1.5* 2.4* 4. 4* 1.42 3.01 1.41 2. 04 1.03 2.02 1.94 2. 15 3.40 4.40 1.27 7. 14 Ri>|iortH only on lipid w*lqht 1***1* l*y MIC. *N* awiple recnlvetl. 'OMAtU* SAMple*. An*lytnl t>y NIC. |T*rtn*i nlMl . 1/JA 4.51 0. 24 0. it) 0. 44 !.<<> 0. 14 0.45 0.29 0. 72 1.47 0. 55 1.94 1.51 5. 17* 2.00 2.42 1.94 2.5 J 2.41 1.74 4. 30 5.01 1.09 5.17 1/7 8 0.22 0.44 0.21 0.25 0. 54 0. 20 0.52 0. 32 0.41 0. 31 0.75* 0.44 2.42 5.41* 1.15 2.20 2.20 4.71 1.90 4.17 4.40 9.17 5.01* 5.57 5/70 * * * * 0.17 * 0.191 0.14 0.12 0.14* 0.4.H* 1.80 5.54* 1.74 0 2.49 3.42 * 0 7.42 1.07 4.01* 4.00 4/70 0.1 2 0.00 0.42 0.4 3 0.12 0.04 0 0.02 0.13 0.37 0.25 0 * 1.02 0 2.10 2.04 1.0) 3.5) 2.72 1.00 3.03 8/70 0.12 0.03 0. 14 0.17 0. 17 0.07 0.11 0.13 0. 14 0.04 0.14 0 0 1.14 2.25 1.47 * 2.10 3.00 3. 20 3.51 1.03 4.23 l 1/70 Fin**! 12/71 0.01 0.09 0.9) 0. 40* 0. 17* 0. 70 0. 40 - 0.74 1.44 0. 54 0.11 0.50 0.27 0. )7 0. 77 0.21 0. JO 0.51 - 0.54 0.7) 0.49 0.44 ). 24 3 09 4.0) 2.24* 4.19 2.75 3.10 2.44 3.09 ) 59 1.47 3. 00 2.24 2.44 1.15 1.51 1.9) 2.01 4.94 1.44 7.4) 3.05 1.42 2.72 HONS 0 0 8 4 0 9 TABLE 5 (continued) (liq/q) MONSANTO RESEARCH CO RPO RATIO N Sample 1.0. Doee Level J7J-1 f S49-K 1 41-M t 291-43 t tl-B l 397-1 t 1JT-I t 70-1 ( Stt-I 79-1 49-1 a 393-1 a Rediua Rediua Rediua Redtun Rediua Rediua Redlu Med1UR Rediua Rediua Rediua Rediua 153-f t 419-H t 329-r l 299-1 t 312-1 t 297-1 f 779-1 ( 3)9-1 ( 794-C 42S-J a 427-J m 999-1 a Hiqh Hiqh lliqh Hiqh Hiqh Hiqh lltqh lliqh Hiqh lliqh lliqh Hiqh Pre-Druq i/n 9.39 9.39 9.99 9.31 9.34 9.43 1.99 9.21 9.94 9.4) 9.11 1.34 9.39 1.99 1.13 0. 33 0.37 1.37 3/77 n 2.74 Lost** 9.49 9.74 1.34 0.44 4.91 17.3 14.4 13.1 2.43 3. 33 19.4 9.44 12.1 14.1 10.3 13.7 10.4 7/77 9/77 4.22 19.1 7.12 3.49 4.13 4.41 34.0 42.1 14.4 13.4 17.4 13.) 19. 3 39.9 37.3 11.4 9.47 14.7 10.3 10.9 12.4 7.34 7.49 12.7 4.40 4.47 7.92 3.44 2.74 11.9 27.9 19.3 32.1 31.1 11. 3 22.1 Prpoc ted only on o ilpid n.l.ht boolo #No Maple received. 'ttitntw eple. Anelyted tTeralnat nlaai. uv 9.3 14.) 19.9 12.4 4.9 4.0 22.7 4.7 14.) 7.) 3.4 13.9 47.7 27.3 21.0 29.9 24.3 24.3 )9. 34.4 34.1 12.1 10.1 21.1 1 1/77 13.0 10.7 72.4* 10.4 7.09 4.90 19.9 4.79 3.12 9.03 3.21 4.94 32.9 9.94 33.3 19.3 20.1 1/79 9.73 14.0 1 ). 4 7.44 7.19 9.19 33.2 13. ) 17.7 13.4 4.92 24.0 41.1* 22.4 31.9 52.7* 24.0 47.) 79. ) 47 ] 37.1 29.9 17.) H.l 3/79 i/i 9.4) 14.3 14.0 22.9 10.3 14.0 20.4 21.5 25.0 20.0 4.40 24.3 29.7 13.2 # t 19.9 11.5 7.9) 37.9 4.041 41.9* j IS.1 ! 77.4* )0 3 54.1 )5. 9 10 1 41-4 3.15 44.9* 1)4 ' 9.971 54.4 115 * 125 132t 122 225* 2.9* 53.3 125 */! 9.49 22.2 39.9 20.9 1)0 14.9 29.2 21.4 21.4 19.2 10.4 3.32 42.9 43.) 55.0 17.7 7.0 23.1 47.) 33.9 74.9 9/79 11/19 11.7 9.42 14. ) 21.3 19.1 14.2 5.94 2.04* 11.1 13.9 9. 30 27.9 13.9 21.9 13.7 15.7 20.9 39.1* 22. J* 5.34 20.9 1.77 12.3 13.7 27.4 39.9 3.47 1.97 172 12.7 4.01 13.1 199* 55.5* 71.9 35.4* 103 24. 11.9 41.4 l nut 12/79 9.9) 19.2 t 9.09 10.4 19.1 24.9 3.39 14.3 27.9 4.27 29.0 . 41.9 39.0 31.) 30.0 - 21.0 21.0 50.4 C z o oOD o o TAIll.E f. <'D CONTENT OF AOIH.T FAT SAMI'l.i::: If I A I: IVFI WITH Oil IGF MAI. fill I I'MI-'NT .SIIIPI'RI) 27 MAIHJII F *l 7 H .`.AMPl.liS) Sample 1 O. Tj|.o flat* MiilUKil U4tf Sanpln Taknn Do Uv i (I Amc lor 1754___ M.-I Nri i|l>l - `J` . I'I'M on HM Wl . Il-lfi 1 *1 I.MJ/Jl . Ll|* d `J FI M on 1. ip i d hi . !>! l a . 404-1 t* 579-H ( 541-M f 419-1 1 7ji-r 424-C r r r r r r 1/27/79 1/27/79 1/27/79 1/27/79 1/27/79 1/27/79 1/29/77 1/29/77 1/24/77 1/74/77 1/10/7 7 1/10/77 Control Control Control Control Cont ml Control 400. 2 24 7.1 < 2U5.1 271.1 411. 1 <211.2 0.5191 0. II 74 0 . )4H5 0.4 154 0.5541 0.1111 0.47 ' U . 74 <0.51 0.44 0.74 1.21 0. HM4 0. 11.1.4 0.2Hf.0 0.11.49 0. C.liH 0. Odl.H 1.00 `1.41 <0.77 077 0.11 <14.0 214-1 r r tow f r 49 l-F f r J01-I f r 444-K ( r 9 11-M l r 424-1 t r 701 - R t r 429-.I n F 412-J r kj o 119-11 m S14-C F F 20-1 545-1 44-1 m $99-1 291-C f 757-1 t 742-C 1 107-1 1 741-E ( 9 41 - M r 211-1 1 F F F F F r F F r r F F F F F 4 7 5- J m r 1/27/79 1/27/79 1/27/79 1/21/79 1/27/79 1/27/79 1/27/79 1/27/79 1/27/79 1/27/79 1/27/79 1/27/19 1/27/79 1/27/79 1/27/79 1/27/79 1/21/79 1/27/79 1/27/79 1/27/79 1/27/79 1/27/74 1/27/74 1/27/74 1/21/79 1/27/79 1/27/14 1/27/74 1/27/79 1/27/74 1/24/77 1/29/77 4/14/7 7* 1/24/17 1/24/71 1/24/71 1/24/77 1/24/77 1/10/77 1/10/77 1/10/77 1/10/7 7 1/10/77 1/10/77 1/10/77 1/10/7 7 1/27/77 1/27/71 1/21/77 1/27/77 1/21/77 1/21/77 1/21/77 1/21/77 1/14/77 1/21/77 1/21/77 1/27/7 1 1/27/71 1/ 10/11 Low Uiw Low Low Low Low Low Low Uw liOM U>W Low | Hd 1 uja Madlura Madia* Hod1u* Ncdlua Medio* Medio* Modia* Hi'dlu* Modi up Nnillut Modlow Ml<yh Hlih M !< Ili<|h Ml<|ll 14 1 <|ll <211.1 <201.2 101.1 144.5 <212.4 <224.5 <2)1.4 <221.4 <275.0 <245.2 445.4 1 15.1 1.022 451.4 <241.1 <2 15.1 441.7 <774.1 <229.1 2)3.9 <254.4 <242.4 <251.4 42.2 444.7 0.1514 0.4 191 0.2271 0.4000 0.7519 0.7414 0. 1794 0.4402 1.0404 0. 15 20 0.5501 1.0217 2.1742 0.4)47 0.7144 0.1451 0.944$ 0. 1900 1. 1040 0.5)04 0.471$ 0 2447 0.4114 0.4491 1.90)1 0.4454 0 4 141 0.4 124 0.121$ 0.41.44 <1.14 <0.12 0.44 0. 1$ <0. 11 <0.10 <0.41 <0.51 <0.24 < l). 70 1.17 0.51 0.4) 1 .14 <0.11 <0.44 0.51 <1.11 <0.21 0.4 1 <0.54 <0.10 <0.50 0.59 0.17 1.1) 1 .04 <0.15 0.50 1 . 17 0.0156 0.SI54 0.2440 0. 2140 0.4929 0.4212 0.2109 0.0141 0.722 0.1449 0.4252 1.2912 2.0774 0. 2449 0.4440 0.0429 0.7100 0.2512 0.0744 0.4404 0.1404 0.JI49 0.2149 0. J104 1.5052 0.4424 0.1100 0.5004 0.7174 0.2140 <2.2 2 <0.11 0. 42 0.41 <0.14 <0.14 <1.10 <2.11 <0. 10 <1.47 1.52 0.7) 0.41 1.20 <0. 14 < J. 74 0.41 <1.01 <0.24 0. 51 <0.71 <0.01 <1.14 0.71 0.44 1.41 1.72 <0.44 0 44 7 . 14 Mol Wl. . ,004 m 47 57 7 94 92 1.5 40 91 109 7) 11 95 54 22 41 42 77 71 99 42 94 lJ 74 44 71 95 74 110 41 74 9) HONS 0 0 8 4 9 1 'M ir ki ll 4lll*'.l 11 Ml on |i.ick.M|r. TABLE 7. PCn CONTENT OF ADULT FAT SAMPLES RECEIVED WITH ORIGINAL SHIPMENT SHIPPED 21 MARCH 197B tMpl 1.0. 719-F m>c 22 7-G m 410-J t?p* r r r t Oat* Shipped 1/27/10 1/21/70 1/27/70 1/27/70 Date Saiaple Taken 5/2/17 5/2/17 5/2/17 3/2/17 Doe* lavrl Ing Aroclor 1254 Control Control Cont rol Control 100.4 <115.5 <101.4 <104.0 Wrt Hp I gilt PH 0.5055 0. <741 1 . 45)5 0.4772 Hf-I f oos-f f 119-H 420-J m 424-1 t lot- t r r r r r r 3/21/70 1/27/10 3/27/70 3/27/70 1/27/10 1/27/70 3/3/77 3/1/11 Vl/H 3/1/17 3/1/17 3/1/77 Low Low Low Low Low Low 125. 420.0 037.0 1.021 451.7 444.7 0.4090 1.1727 1.1042 1.1124 1.0507 1.0540 101-1 f 757-1 t 40-1 747-0 t S40-H f 545-1 m r F |f 1f F F 1/27/70 1/27/70 3/27/70 1/27/70 3/27/70 1/27/70 5/1/17 3/3/77 3/4/17 3/1/77 5/1/77 5/4/77 NeiUw Mediate Mediwai Medium Medline Medium 001.2 700.0 527.2 2,030 4.244 2.400 1.1450 0.2794 1.2044 1.5172 1.5490 0.409) 770-1 f 000-1 m 700-1 f 700-0 425-J 220-r ( 4J7-J m i52-r i 1)0-1 1 207-1 1 ii2-i t 410-N f F F F F |r lr F F F F 3/27/70 3/27/70 3/27/70 3/27/70 1/27/70 1/27/70 3/27/70 1/27/70 1/27/70 1/27/70 1/27/70 1/27/70 3/4/77 3/4/17 5/4/77 3/4/77 5/4/17 5/4/17 3/4/77 5/4/77 5/4/77 3/4/77 5/4/77 5/4/71 High High Mlc|h Hl<jh High High High High High High High High 7.490 12.300 2.492 11.070 7.411 11.540 7.490 11.120 13.100 11.120 0.420 21.500 0.0417 1.1740 0.0750 0.4010 0.7)00 1.0)31 0.5404 0.4441 1.20)) 0.4159 1.4097 1.4)50 Lipid weight deterwlnatlone for thee# a|>U were destroyed. Hc*t Wt. 0.19 i a l"l/g> 0. )) `0.2) `0.12 <0.34 0.10 0.31 0.59 0.95 0.4) 0.42 0.40 0.74 0.44 1.14 2.74 4.91 0.44 10.4 2.05 U.l 10.5 1). 1 11.7 17.5 12.0 10.4 5.15 14.4 Lipid Weight 0.2400 0.2214 1.2414 0.1)00 0.5494 1.0500 0.00)2 1.1474 0.0540 0.0410 l.1470 0.0124 1.02)2 1.1)40 1.1124 0. )))2 0.7204 0.9740 0.5410 0. )4)2 0.29)2 0.0044 * 444 Lipid Mt. ftaala 0.50 <0,52 <0.15 <0.50 0.22 0. 40 0.02 1.19 0.3) 0.51 0.49 4.14 0.52 1.51 1.02 7.20 10.4 12.0 4.55 )0. 5 24. 2 14.0 " " " wet wt. " m 51 43 14 49 0) 77 71 00 01 02 04 12 05 00 72 40 01 0) 41 5) 40 70 ~ ?6*BOO SNOM tabu: fl. ITU CONTENT Ol* ADULT .SAMIU.ir. photi::; r;n> by MIC AND CllMOMATO(;KAI*m:i) IlY MRC: SMIl'Pia) 2? MARCH 197 a o{7 s#p|r 1.1>. rviif nte Shipiwd |n<) Date Onae Arnclor Sample TakenlevcII1S_4 . PPM on Wot Wot Wt WPiqht n.-\nin .....isL.__Ijlj/'jL l.i pi it Hoiijht I*PH on Lipid wt Basis *iiUvj/uJ_ l.ipici Wt Wet' STT a 100% _______ m 200-r i 274 -T t 547-0 r 5/i-o r KN>J 404-1 f 1.11-1 t 744-C f VIVM 3/27/71 1/27/71 3/22/71 3/27/71 3/27/21 3/27/11 S/2/77 S/2/77 S/1/17 S/2/17 S/2/77 S/2/77 S/2/71 Contioi Contra! Control Cont roi Control Cont rol Contrnl 1)7.1 114.2 511.7 241.7 452.1 411.1 41). I 1.154** 0.44/7 1.0)54 0.4270 0.1210 1.4440 0. !<> 0.27 0.14 0.44 0.57 0.54 0.44 0.54 0.4201 0.1464 00..17S40704 0.5412 1.4055 0.755) 0.14 111 00..41 0.14 0.45 0.45 i-r ( n <J>D U `Sample type not indicated on vials received from NIC. `Instructions fnw HiC statud to disc.wd this nample ilue to p.utial apillaje. oJt 0 11 II 41 47 44f 151 HONS 0 0 8 4 9 3 MONSANTO RESEARCH CO RPO RATIO N TABLE 9. PCD CONTENT OF ADULT FAT SAMPLES RECF.IVED WITH 0R1GINAI. SHIPMENT SHI m:n 27 MARCH 197B Staple 1.0. m-c 744-E t 227-C m 410-J m 719-F a SIM ( 2o-f t *4-1 f 274-f t 57*-N f )H-H f 7-F t 0J9-H ( 701-E t 7*4-1 1 4iJ-r f Sll-C a 432-J 2IH! ( 4i-H r 713-S ( 213-1 ( 599-1 * 20- 1 m 4M-N r 330-1 f 7*7-1 f 770-1 1 152-F f 724-r f 312-1 r 2*7-1 1 475-J Tfpe r r t r r r F F F F r r F r F F F F F F F F F F f F F F r r F F F F F oat Skipped 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/2t/7* 3/27/7* 3/17/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* 3/27/7* Date Sample Taken 7/7/77 7/5/77 7/7/77 7/7/77 7/7/77 7/5/77 7/5/77 7/3/77 7/1/77 7/5/77 7/3/77 7/5/77 7/5/77 7/3/77 7/5/77 7/3/77 7/7/77 7/7/77 7/4/77 7/4/77 7/4/77 7/4/77 * 7/4/77 7/4/77 7/4/77 7/4/77 7/4/77 7/4/77 7/4/77 7/4/77 * Done Level Inq hroclor 1254 Control Control Control Control Control Control Control Control Control Control Control Control 443.4 271.5 324.9 341.9 359.0 2,*01 314.9 3*4.3 312.4 1,141 3*1.3 22*. 1 Low Low Low Low Low Low 341.3 42*.7 330. 3 1.21* 430.* 144.2 Medium Median Median Median Medium Medium 3.9*2 5,094 1,332 2,777 4,759 3,327 Hlqh Hlqh lliqh lllqh lliqh lllqh lllqh Hlqh Hlqh lllqh lllqh Hlqh 24,*10 23,540 4,151 10,790 23,410 12,2*0 9,4*1 4,77* 30,5*0 33,420 7,771 0,4*9 M** 1 Nei<|ht Iqi 0.4*09 0.4447 1.1713 0.5491 0.4794 0.7404 0.4929 0.3773 0.3370 0.5173' 0.5*7* 0.342* 0.5991 0.3473 0.2537 0.7344 0.499* *. 3M7 *.3094 0.5030 0.3*20 0.4440 0.7741 0.75)7 0.4749 0.5901 0.5153 *.5344 0.4554 0.742* 0.5514 *.4333 *.*174 2.0000 *.1971 0.7557 FPM on Net Wt. an a iu`j/q) 0.74 0.50 0 47 4.92 0.73 3.*9 0.44 o.ai 0.93 2.23 0.40 0,4) 0.57 1.17 1.3* 1.24 { 0.4) 7.*2 10.1 3.41 4.22 4.1) 4.41 ,39.9 1)9. 34.0 14.4 17.4 15.4 37.5 14.7 11.4 l.lpid Met qht 0.14)4 0.3700 0.9)00 0.4)12 0.3400 0.4)4* 0.411* 0.2112 0.4240 0.4092 0.4)04 0.2972 0.5040 0.7))2 0.09*1 0.2972 0.34)2 0.1100 0.4097 0.373* 0.3077 0.35*0 0.4144 0.4*00 0.4100 0.34IO 0.0992 0.3710 0.4144 0.444* 0.347* 0.4)0* 1.494* FFH on Lipid wt. Basts 2.77 0.7) 0.37 1.24 1.0) 4.52 0.74 1.45 1.20 2.79 4.70 0.77 0.47 1.(4 3.55 4.74 1.03 1.50 9.73 13.7 4.31 7.74 7.75 4.19 45.4 4).l 49.1 77.2 Lipid Mt. Wat Nt. a 1*01 19) 27 79 02 7) 71 *4 5 54 77 10 04 02 03 4) 39 34 4* 29 10 44 0 0 79 90 45 42 19 71 ftO no arlilon dale Mated on aample pnckaqe. + r TAIII.C 10 pen CONTI'.NT OK ADUI.T FAT AND OMIiNTUM r.AMPl.HS IlF.CKIVI'O with oiur.iriAi. siiiphkmt snii*pi;i> n march mo S*m1 tint* .D Typ* Sh lowed 0te Semple Taken DOH Level |nq Aroclor 1254 Wot P I'M on wot wt Han in WJ^iL pim on l.ipid wt HcKjht Rah m _*3l________ !u'J/ilL- 1.11> i d_ *`1 Wt 410-J 227-0 200-r I 509-M r 274-f f 570-11 t r r r r r t 3/27/70 3/17/70 3/27/70 3/27/70 3/27/70 3/27/7# 1/4/71 0/4/1? 0/1/71 0/1/77 0/1/77 0/1/77 Control Control Control Control Control Control 114.1 209. 1 310.1 425.2 175.0 1,179 0.4709 0.9095 2.0117 2.1071 2.0102 0.5051 0.24 0.27 0.16 0.15 0.09 2.02 0.1412 0.7112 1.4100 2.1)40 0.1500 0.1020 0.11 0.2? 0.20 0.10 0.21 1.00 514-C m r 3/27/70 //77 Low 100.0 0.1921 0.20 0.5540 0.29 t 119-H m r 3/27/70 0/4/77 1.0* 2,740 1.1477 2.19 0.9244 2.97 o 420-J m r 3/27/70 0/0/7? Low 05.05 0.9)19 0.09 0.7144 0.12 412-4 m r i/ti/n 0/0/77 low 109.3 0.1109 0.27 0.1040 0.92 405-r t r 3/27/70 0/1/17 Low 1,009 1.4141 0.70 1.1744 0.91 204-1 i 0 3/27/70 0/1/17 low 10,450 1.0271 0.42 0.1000 14.0 o 039-11 t 440-E f r r 3/27/70 3/27/70 0/1/17 0/1/7? low Low 4,020 03.93 1. 1500 0.0102 2.90 0.10 1.1052 0.0504 1.04 0.1) m (A 4#3-r i 109-1 ( 701-C r o r r 3/27/7# 3/27/70 3/27/7# 0/1/17 0/1/71 0/1/7? ; Low low Low 100.1 204.1 100.3 0.4190 1.5710 0.0000 0.30 0.1) 0.24 0.1744 1.1240 0.4504 0.92 0.15 0.15 n9 024-1 ( r 3/27/70 0/1/1? I Low 150.1 0.5039 0.27 0.4000 0.32 2 73-1 r 1/27/7# 0/2/17 Medium 0.100 0.5090 10.5 0.5000 12.1 141-H r r J/27/70 0/2/77 Medium 7,210 0.5017 12.4 0.3072 19.7 no 540-11 ( r 3/27/71 0/2/77 Med 1 um 0. 3)5 0.174) 10.0 0.59)0 14.0 9 oV f 757-1 t 703-E r f r r 3/27/70 1/27/70 1/27/70 3/27/7# 0/2/77 0/2/77 0/2/77 0/2/17 Medium Medium Medium Medium 0.009 1.024 4.902 5,507 1.0700 0.3529 0.00)1 0.4410 4.00 12.7 0.7720 0.1000 0.5040 1.2400 10.4 0.69 0.70 4.50 t 599-1 m r r r r f 3/27/70 3/27/70 3/27/70 1/J 7/70 1/27/7# 0/2/71 0/4/1? 0/0/17 0/4/17 0/4/1? i i 1 Medium Medium Medium Med1um Medium 5.10) 1.06 J 12,590 0.595 1,527 1.1405 1. 1)45 1.0000 11.0 5.00 0.0100 1.1)60 0.0004 1.1100 0.1404 0.2) 1.22 14.) 0.50 10.4 r 000-1 m r 1/27/70 1/27/70 1/27/70 1/27/70 1/27/70 1/21/70 0/2/17 0/4/17 0/2/17 0/2/17 0/2/17 0/4/17 lllqlt lliqll ll i<ih II t-llt lllqll 111 <|tl 24,050 10,050 10,090 10,0)0 20.190 10.2)0 1.1000 0.1052 10.5 1.1120 1.2092 0.2060 0.4400 0.5052 1.1400 21.0 11.0 01.0 00.0 40.0 20.3 HONS 0 0 8 4 9 5 TABLE 11 PCB CONTENT OF ADULT FAT AND OMENTUM SAMPLES RECEIVED WITH ORIGINAL SHIPMENT SHIPPED 27 MARCH H7B Sawpia Dai* l.D. Typo Shipped Dai* SaApia Taken Doae Level ** Aroclor 1254 w*t MMijht tql fW on WPt wt. Imo/oI Lipid WCi'iht <q PPH on Lipid Wt. Aaa i g Lipid *>t. MPt Wt. * IMS m 7-r f m-r f 209-r ( 7IM t 227-G A 119-r a f r r r r r 3/27/7* 1/27/7* 1/27/7* 1/27/7* 1/27/7* 1/27/7* 11/1/77 11/1/77 11/1/77 11/1/77 10/11/7? l*/Jl/77 Control Control Control Control Control Control 90.29 140.1 7*. *2 Hi.* <194.4 210.2 0.4014 0.4*74 4.4324 1.0144 4 .*245 0.7257 4.11 0.20 0.11 0.11 <0.24 0.29 4.7044 0.5140 0.5)12 0.7944 0.4444 0.4404 0.11 0.27 0.1) 0.15 <0. 29 0.40 10 75 14 7* 3 41 MONSANTO RESEARCH CO RPO RATIO N 421-J a r 1/27/7* 514-G A r J/2T/7* )9-l f r 3/27/7* (H-r f r 1/27/7* ii-i r r 1/27/7* 119*11 A r 3/27/7* 4J2-J A p 1/27/7* ii-r r r 1/27/7* 244-1 r r 1/27/7* 444-E ( r 1/27/7* 741-E ( r 1/27/7* ro 1)9-11 1 r 3/27/7* Ln 301-1 t 540-11 f 757-1 f 743-E I 273-1 1 201-G f 595-1 * 549-1 a r r r a r w T t T r r F 1/27/7* 1/27/7* 1/27/7* J/27/7* 3/27/7* 1/27/7* 1/27/7* 1/27/71 3/27/7* 1/27/7* 1/27/74 1/27/7* 19/11/77 10/71/7* U/l/77 n/i/n U/l/77 10/11/77 10/31/77 U/l/77 U/l/77 U/l/77 U/l/77 U/l/77 11/2/77 11/2/77 11/2/77 U/2/77 11/2/77 11/2/77 11/2/77 10/31/77 10/31/77 10/31/77 10/31/77 Low Low Low Low Low Low Low Low Low Low Low Low 5.95* 2,591 1.119 2,409 2,124 1,995 1,0)1 1,494 1,542 1,222 1,444 2,424 H*dJu NodlllA Hedlua HPlIlUR NadiuA HediUA N*dlUM Hedtu* HediUA Medtun Hedlun HediUA 3,0*0 4,472 10,720 13,100 4.355 *,509 4,721 5,099 1,(09 1,930 3.407 4,409 0.4J17 0.7424 0.*014 1.2414 1.11*5 0.90*2 0. f151 0.5421 0.5511 0.7250 0.5117 1.4151 0,4419 0.4254 0.5194 0.4144 0.4943 0.4570 0.4343 0.5799 1.1*44 0.94*4 0.7414 0.4414 7.14 3.40 1.42 1.94 2.15 4.40 1.27 3.01 2.12 1.49 2.*4 1.41 4.40 10.1 19.9 22.4 7.09 13.0 10.4 1.79 3.21 9.03 4.44 5.12 0.1472 0.4504 0.1554 1.4440 1.7132 0.1)** 0.432* 0.24 14 0.3220 0.0744 0.4340 2.0414 0.5)24 0.5400 0.3400 0.4)14 0.1494 0.5444 0.5444 0.4004 0.0774 0.1412 4.44 5.15 1.51 1.47 1.45 5.41 1.4) 5.97 4.45 1.40 3. 34 1.24 5.79 11.9 31.5 0.4* 15* 15.5 #00-1 A V 1/27/7* 10/31/71 Hlqh 17,14* 0.4524 20. 1 0.1714 25. 5 ^ oX r 1/77/7* J/27/7* 10/11/77 11/2/77 II i <|h It t |l ** 11,000 - 0.5079 ** 2 3/27/7* 11/2/77 II 1 iflt 9,954 1.1242 4.44 0.9700 is* 3/27/7* U/l/77 Hiqh 11.4 74 0.7294 11.5 414-11 f r V77/7* 11/2/77 23,450 0.7194 32.9 Od * o- Ho er19Ion data IllUd on <>! (tackle. *SaM||e l*iroped. 104 59 94 1)2 130 1 11 50 5* 121 15 145 1) 90 43 04 19 !i MONSANTO WESEAUCH C O *p O AT NJ Ol 3 V o oa *%> TABLE 12 _ l .0.__ Tjl< UI4 jn-c o-j ii*-r ni-r 1*4-0 4*4-1 4*9-14 i#o-r >-r 4i*-i su-m O r t r tr (f ff It tr tr tr rr i*i- r 7*4-1 i 4*w r 47 9 -1 I 1*9-1 f 44*-e i 4*i-r t 19-0 ( 470-J *14-6 IM-I 417-J )oi-i r i*i-t i 10-1 m 041-H i IM-I I i4-i 777-1 * 994-1 747-f i 499 -t 7*i-r. t 440-n r t r r r f r o f r r r t r r f r f r r r r r r r 149-1 t 9141-r f 147-r I 101-1 I 7P7il4l--Ir II 111-1 I 474 .7 4/t-J m 4 4*- It I f**-. a 4*0-1 a tP - r I1 t t t I at* SMOpo# 1/21/70 i/ji/71 )/3?/7* 1/11/1* 1/27/11 1/11/7* i/n/7* 1/11/it i/n/7* 1/17/7* j/77/7* l/11/i* 1/71/1* l/n/l* 1/11/1* J/ii/i* )/ii/i* 1/71/1* 1/71/1* 1/71/7* 1/11/1* 1/71/1# J/71/10 1/31/70 1/77/70 1/11/70 I/I 7/70 1/11/10 1/71/70 1/77/10 1/31/10 J/37/10 1/21/1# 1/11/1# 1/71/7* 1/21/70 1/21/1* 1/11/1* 11//1117//770* 1/11/10 1/17/10 1/17/70 1/11/7# 1/71/70 1/17/1* PCB 1'ftMTF.HT OF AfHH.T FAT AH1) OMF.NTIIM SAHl'I.F.f* HF.C'I'.IVF.D WITH OIUCINAl. SHIPMENT SHIPPED 27 MAIICII 1978 Data Sa*la Tafcrn 1/4/1* 1/4/1* 1/19/7* 1/4/1* l/l/l* 1/1/1* 1/1/7* 1/1/71 l/l/l* 1/1/1* 1/1/1* 1/1/1* 1/1/1* 11//11//11** 1/1/1* 11//11//111* 1/1/1* l/l/l* 1/10/7* l/S/1* 1/4/1* 1/4/1* 1/4/1* l//7# 1/4/7* 1/4/1* 1/4/1* 1/4/1* 1/4/7# 1/10/1# 1/4/1* I/VI* 1/4/7* 1/4/1* 1/4/7* 1/4/7* 1/4/1* 1/4/1* 1/4/7* 1/1/7* 1/1/7* I /!*/ ! 1/4/7* 1/4/70 1/4/1* 1/4/1* Dour Laval Control Condo I Control Condol Control Control Control Control Control Control Control Control Lm LI.OoWw low Il<.OOWW Com Low Lam Lam Low Lom NrdliM HtdiuA MrdliiA MnlluM HPtroidl ItuuMm Ptrillun H*.l I >Mk Hr<t t Hnlliia Ptrdtnn IMlIilN Mlqh HI,,It tit.lt Hl.fll Itflll HI'IH HI.,It HI,til Hl'Mt Hull ltl.,1, Aiorlor Mfi-ilil I i'll_____ t.jl 1,99* llV 11* *41. 7 1*. 1.11* 7 17 111 417 177 777 1*9 1.4*9* 9. IN* I 0- 1**4 0-SI l 0.4411 1.MI1 a.7*A0 0.* 14 7 0.7147 1.7017 #.4901 0.11*1 1.909 1,440 1,940 7,100 1.410 7.410 4.10* 1.M7 7,99* 2,717 1,402 1,30) 0.1711 0.44*4 O.fSlO 1.1)14 I* 701 0**** 1.0114 0-947* 04*07 0.4147 0.S14 0-4)71 9)1 1.7174 0*1 0.9111 4*1 0.49 17 741 0. 1914 040 9. *4 *,* 14* 1.1*91 10 4 0-9410 990 9.4*1* 490 7.4104 740 0-**71 794 0.40*4 110 0-4944 410 0.4191 7 10 0.7490 900 2.4100 410 *.!! J 10 1.0140 1*0 * . 194 I 490 1 . > 7** 700 0 47*1 4 I* .t)71 94 1 *. 19* t ** *1914 9 10 t.fcIV, n-w - Ml . Ju'j/'l). A 44 0 )7 1.94 I.*7 I.*fc t.M 0. 10 0.1* 0*4 0.79 g.7* 0.44 7.47 1.41 7.41 1.74 l.SI 7.0* 4. 17 1.94 4. I P 4. 10 4.*) 1.09 *19 7 19 1)4 11.4 II 1 4.91 9 74 74.0 14 1 17.7 7.4* 14.U 47.7 *7. 1 41. I 41. 1 11 9 74 I 74.0 19. 17. I 77.4 17.0 040 1.1 ( 1.1 Me.,1,1 I .4**4 0.11*0 0.71 17 0.77** 0.794* 0.4047 0.494* 0.7444 0.479* 1.1000 0.7904 0.71*0 0.4404 0.4400 0.4*70 7.14*0 0.9100 0.7*11 0. WOO 0.1*00 0.417* 0.7400 0.1191 0.1740 0.9970 0. 0.419* 0.74*4 4400 9.9*04 *.*971 *.1447 1.1*44 0.41UO 0. 1 1)4 0.41*0 .440# 0 2 1/0 I 4*04 01 ,7 0. 1411 1.014* *.lll *.744* *.174/ a. i*74 U.40 12 Pm Ai Ml . IMai Wrl W| . . 1 10*1 JaH/jlj i )I 0191 40 )7 *7 4 74 41 74 II 9 47t 49 44 74 t\ II I 4* l 41 4 4* 14 14 74 4I tj MONSANTO RESEARCH CO RPO RATIO N TABLE 13. PCD CONTENT OP ADULT FAT AND OMENTUM SAMPLES RECEIVED WITH ORIGINAL SHIPMENT SHIPPED 21 MARCH 1078 llftftpl ft 1.0. DtK Oat* Typ* SkliftH (iftlf Tak** (too U*l H Nrocinr 1)4 PI'M Oft Oft Wat W*l Wt. tlpld tlpld wt. Mr in lit tor* l<|lit _`9`_____ J'."J/9*_ ____ !)!_____ I..9/3I Ur.1' 100, 131 414-1 t 214-r ( u-e f 494-1 | i-r t 341-M 1 314-H ( 100-F ( 414 *J ft 111-C ni-r m m-c r r r r r r F F F F r 0 1/11/79 1/11/10 1/11/10 1/21/10 1/19/10 l/lt/10 1/19/1* 1/19/14 3/19/1* 3/19/1* // 3/19/1# )/i/ia 1/4/19 1/4/79 1/4/19 1/4/19 1/4/19 1/4/19 1/4/19 1/9/19 1/9/19 1/9/19 1/9/19 Control Control Control Control Control Control Control Control Com ml Control Control <ai.a <!- 114.4 It). 1 <111.4 <114.4 111.9 <)}.) 199. 9 419. 4 112.9 <499.4 loin 4. 7 >41 1.fan 1.1174 0.4444 4.MII 9.1401 0.019) 4.91)2 9.4417 1.9429 9.3120 <0.44 <0.34 9.11 0 11 <0.11 <9.24 9.13 <0.31 0. 44 0.41 0. 11 <0.13 0 4411 0 3432 1.13 12 0.1444 0.7404 0. 7144 4.4414 0.4412 0.2417 0.1414 0 34W 0.0)92 <0.32 <0. 74 4. 20 0.27 <0.4| <0. 13 4.12 <4.4$ i. 1$ 4.3$ 4.41 <14.2 03 It 74 11 11 0 11 71 14 3 $ 7.4 141-9 1 F 3/19/7# 1/4/19 toa* 2.944 0.9440 2.20 0.10)1 2.1* 14 104-1 1 r vv 4)1-11 f F J/n/n 1/9/19 1/4/1* 108 low 1,114 1.411 9.1942 9.44)1 4.71 2.20 0.31)4 0 4344 *.41 ).*$ T) 0) 43-r r F i/n/ii 1/4/19 tow 1,199 1.4131 1.94 1.4*44 2.44 41 K> Nj 44i-r r 4)4-1 r 44-e ( t lll-N ft 4ll-J ft me * 414-J ft o F F r r o F F )/i/'* 3/11/9# v/ 3/21/19 3/11/9* 1/11/1* V/i* V>V?9 1/4/19 1/4/19 1/4/19 1/4/19 1/9/19 1/4/19 1/9/14 3/9/1* tow law low low tow law tow tow 4.944 1,244 1.91$ 4.294 3.4)4 4.7141 0.4717 1.2440 4. 1119 9.7441 4.1)44 4.3449 1.0)44 3.41 4 11 1.13 2.4] 1.71 3.1) 4.10 $.51 9.4*1* 1.4192 1.0*31 0.3934 9.1414 0.2309 4.2049 0.31*4 1.41 2.71 1.1) 2.91 20.2 11.1 17.4 10.0 44 210 47 4) 44 14 11 92 i*2-e f 301-1 f 14 1 -H t 311-1 t 141-K 1 S4o-h r 449-1 a 494-1 44- 1 ft J41-I t 499-1 ft 14 j - r t F F F F F F F F F F O f F r r o F F O F F F v/ 1/11/1# J/H/14 J/11/T# 1/21/1# J/IVU 1/21/1# )/9/l* l/91/* ]// J/J2/1# 1/11/19 i/11/19 1/11/19 1/91/10 1/1V19 1/11/1* 1/11/19 1/11/lf l/H/19 1/H/ It 1/11/19 1/11/19 1/4/19 1/1/14 1/1/1* 1/1/14 1/1/1* 1/1/1* 1/1/19 1/4/79 1/4/14 1/1/14 1/9/19 1/9/1* 1/1/19 1/1/19 1/1/19 1/9/19 1/9/19 1/4/14 1/1/19 1/9/19 1/1/19 1/9/14 1/4/14 1/11/14 Tt NftOlun MftOlun Hftillwft HrdlMM Nrdlua Hfttlluft Hfttllun ffodluft Nrdlua Mftillwft MndllW Mdluft Mlqft Nlqh Itlqh Hlqh Hlqh Klqll Hlqh Htqh NlqH Nlqtl Mlqh 19.4)4 11.449 1. 919 19. 110 11.119 23.319 2).190 11,414 4. J13 9.4044 4.4119 4.4110 1.0113 1.J2I2 l.lll) 4.9441 I.IM4 1. 1340 4.4179 o.om 1.0114 0.3431 0.100} 1.4403 0.4411 4.1401 4.3411 1.1104 9.4444 4.3314 1.1111 0 7411 0.3971 21.3 14.4 14.4 1.41 10.3 14.3 20.4 22.1 10.0 23.0 24.3 0. 40 41.9 13.1 40.1 34.1 43.9 77.4 70. 1 3.15 *4.9 1 74 4.04 9.3111 0.1012 0.4000 0.43)4 1.1*04 4.4012 0.1040 0.9)40 1.4144 0.1740 0.1040 0.4724 4.2134 4.0404 0-0740 4.4220 *.9I2 0.2440 13214 .042 0. 1240 *.$340 4.2944 4.0032 >11 17.4 19.) 11.1 12.0 20.2 24.0 27.) 27.4 ) 1. * 111 10.3 13* 117 11.3 10.* 51.1 144 14.4 14.0 111 10* 141 tat 4$ 37 12 41 19 41 11 94 43 71 40 40 l 1.0 HONS 0 0 8 4 9 8 TAni.r. H PCD CONTF.NT OF ADULT FAT AND OMF.NTUM SAMPI.F.S SIIIPPFD 4 MAY 1978 Dtr Shimwd 0*1* SMftlc taken Dob* Levc 1 t IK) Aroclor 1254 M*t M* l/jht mi I'I'H on H*t Mt . li.t a i a li'9ta! l.ipi.l W*M|llt ____U> (I'M on HI . li t a t n <M4/`1 Lipid W*l m S69-M t n9-r m 211-G m 4lt-J 426-C r F F O 5/4/11 5/4/11 5/4/11 5/4/11 5/4/7* 5/1/1* 4/2*/7* 4/7N/7I 4/2S/7R 4/2*/7* Control Control Control Control Control <21* . 8 <157.2 162.4 100.Q < 252.4 1.2701 0.4981 0.9901 0.5714 0.6912 <0.17 <0. 12 0.16 0.6* <0.16 1.066* 0. 2 156 0.794* 0.2216 0. 1012 <0.20 <0.67 0.20 1 . 69 < 2.45 4 *0 40 IS 19-H 1 109-1 f 446-C r 420-J 4 12 -J m SI6-G a 24-i r 4U1-F f F F F F 0 F F F O 5/4/7* 5/4/7* 5/4/7* 5/4/7* 5/4/7* 5/4/7* 5/4/71 5/4/7* 5/4/jl* 5/1/7* 5/1/1* 5/1/1* 4/2*/7* 4/2*/7* 4/2*/7* 4/2*/7* 5/1/7* 5/1/7* Low Low Low Low IfOw I.OW liow IjOW Low 1,797 1,450 1,004 1,506 2,*67 4,608 2, 171 1.2*2 1,655 1.4089 0,9167 0.5729 0.5046 0.715* 0.6212 0.5155 0.174 7 0.29H6 2.69 l.0 1.76 6.00 4.01 7.42 1.42 5.54 1.1904 0.7296 0.4252 0.146* 0.1540 0.2516 0.2210 0.1196 1.19 2.26 2. 17 10. 1 IB. 1 5.6/ 11.8 4 S9S-I a jo2-1 r F O 241-1 1 1 5/4/7* 5/4/1* 5/4/7* 5/4/7* 5/4/7* 5/4/7* 5/4/7* 5/4/7* 5/4/J7* 5/4/0* 5/4/7* 5/4/7* 5/4/7* 5/4/7* 5/1/7* 4/21/7* 4/27/7* 4/27/ /* 4/27/7* 5/1/7* 4/27/78 4/27/7* 4/27/1.* 4/7 1/ Ml 4/27/78 4/27/7* 4/2 7/7* 4/2 7/ 7H Heriiua Kc.IIum NtMlilW Ni'il 1 ua K**l l tIM H*tl i UIB llM|h IIm<I> lll<(h II M|lt II Mill II M|lt II M|lt II M|ll 11,600 10,800 18,950 7. 105 I4,llil 15,400 111,400 77,410 46,91.0 127,400 81,21.0 106, 100 74, r.'iu 49,050 1.1296 0.571* 0.500* 0.9 1 10 1.0*15 l . 16 10 1.051* 0.415* 0.5166 1.5 156 0. bla 7 5 0.117 IN 1 . J1 `Mi 0.4/71 29.7 i*.* 17.* 7.8 1 n. 5 112 125 1/2 0.9156 0.4028 0.*652 0.1756 15.9 150 1) 16 HI) 66 HONS 0 0 8 4 9 9 TABLE 15 . CONTENT OF ADULT FAT SAMPLES SHIPPED 15 JUNE 1978 MONSANTO NC5CANCH CONVOCATION tiapli 1.0 404-1 t 414-1 1 144-E t J74-F t 549-H 1 434-C n 139-F m 4IE-J m 221-G m 405-F f 444-C f 434-1 1 39-H f S14-C 119-H 4J2-J 420-J 743-E 211-G 41-H 747-1 347-1 f r f r f r f r 415-1 30-1 r r r r 704-K Typm r r r r r t r r F r F F r t F F F F F F F F F r F r r r r r Oats Shipped 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 */*/ 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 4/15/74 D*t Semple Taken 5/34/71 4/4/71 5/34/71 5/34/71 4/4/71 4/4/74 4/4/71 4/4/74 4/4/14 4/4/74 4/1/74 5/34/74 4/4/74 4/4/74 4/4/74 4/4/74 4/4/71 4/5/74 4/5/74 4/5/74 5/34/74 4/5/74 4/5/74 4/5/74 4/5/74 4/4/74 4/4/74 4/4/74 4/4/74 4/5/74 4/5/74 4/5/74 4/5/74 4/5/74 4/5/74 4/5/74 4/5/74 4/5/74 Doee Level nq Aroclor 1354 Control Control Control Control Control Control Control Control Control 330 40.4 44.4 44.3 44.0 73.1 34.4 10.0 34.0 Low Low Low Low Low Low Low Low 1.054 i.m 450.4 2.134 1.292 1.433 4.l 1.443 Median Median Medlue Median Med Ion Median Median Median Median Median Median Medlun 7,135 4.947 4,474 19,410 14.530 13,470 12,040 0,500 4,053 743.2 20.410 13.200 Hlqh High High High Nigh Nigh Nigh High High 35.404 21,410 4,375 14,470 33.000 14.220 20,030 39,730 7.500 Met weight l9t 0.5140 ).0414 0.5404 0.5475 1.1524 0.1940 4.3021 0.3210 1.4447 0.9093 9.7244 4.9142 1.0491 0.3455 0.4730 0.3457 0.4444 0.5447 0.5700 0.4049 0.5)05 0.4977 0.0542 0.5419 0.30)7 0.7749 0.32)7 0.9445 0.7243 0.4441 0.4947 0.3411 0.4)14 0.3044 0.1431 0.5900 0.5)05 0.324) ITM on Met wr. ft.mli (./si 0.43 0.04 0.12 0.12 0.04 0.17 0.13 0.25 0.02 2.04 1.02 1.0) 2.10 3.53 2.72 1.40 3.1) no 4.44 34.4 34.4 29.2 14.1 21.4 22.2 10.4 3.32 21.4 10.2 55.0 43.3 17. 7 42.0 07.0 47. 3 33.9 74.9 23.1 Lipid Weight 191 0.3412 0.1 M4 0.43)2 0.4044 1.1044 0.0)44 0.07)4 0.1444 1.54)4 0.1120 0.5792 0.72)2 0.7474 0.1420 0.23)4 0.0040 0.3444 0.3094 0.3920 0.2)44 0.3494 0.3240 0.4792 0.2044 0.2924 0.4154 0.0000 0.5094 0.4940 0.0)04 0.20)4 PPM on Lipid wt. nsin l.g/.l 0.09 0.09 0.14 0.17 0.04 2.34 0.49 0.49 0.02 2.40 2.27 i.31 2.0$ 7.94 7.04 1.7 3 5.41 11.) 12.4 35.0 53.4 44.9 10.7 50.1 39. 1 13.1 35.1 Lipid Wt. Met Wt. " m 70 4 77 70 94 14 74 51 94 70 00 79 74 45 35 24 71 11 49 50 72 45 71.7 77 5. 14 HONS 0 0 8 5 0 0 TABLE 16. PCH CONTENT OF ADULT FAT SAMPI.F S Sill 1*1 'i:n 2 4 AUGUST 107 8 M O N S A N TO R E S E A R C H C O R P O R A TIO N Staple i .to. sii-u r 764-E r S7*-m r (04-1 t (ll-I f 569-11 t 2U-r f aoo-r f 719-F 227-G * 411-J a 426-G 271-1 1 757-1 f 761-E f 540-11 r 211-c r 107-1 1 46-1 R ?n?-i # 112-1 1 2(9-1 i 22(1 f j/o-7 t 4 27-J (00-1 42S-J TYP r F r r F F F F F F F F F F F F F F F F F F 7 1 F F D*t Shipped /i/io 1/24/70 0/24/70 /24/70 0/24/70 0/24/70 0/24/70 0/24/70 0/24/70 0/24/70 0/24/70 0/24/70 0/24/70 0/24/70 0/24/70 0/24/7* 0/24/70 0/24/70 0/24/70 0/24/70 0/24/70 0/24/70 0/24/70 0/24/70 0/24/70 0/24/70 0/24/70 0/24/70 0/24/70 0/24/70 0/24/70 nte s**ple Token 4/14/70 7/11/70 7/31/70 7/11/70 7/11/70 7/11/70 7/11/70 1/11/20 0/1/70 0/1/70 0/1/70 0/1/70 0/1/70 0/1/70 7/11/70 0/1/70 0/1/70 0/1/70 0/1/70 0/2/70 0/2/70 0/2/70 0/2/70 0/6/70 M/1/ / H H/I/7H 0/1/70 0/1/70 i/2/70 0/2/7 M 0/2/70 Done* Uv> 1 lU| Aroclor 1254 Cont Mil Cuntrol Cunt ri>l Control Cont mi Control Control Conirul Cont rol Cont rol Control Cont rol 106.0 71.2 56.0 50.0 27.0 69.2 59.6 90.4 75.6 09.2 62.4 17.6 Mod ill* Medin* Med t ii* Med in* Medihr Medium Mol i ti* Medium Medium Medium Mediuh 9.632 1,771 10,000 5,220 1,7)0 15,940 1 1,420 4,090 1,910 (.680 8, 1)0 l i |li llnili II Kill M Kll IlKltl IIK|il II i>|ll 1,144 11, 4 0 15, 100 1.90 1 4U0.0 7,051 7,62h 5,050 Met He i <}tit J'j) . 0.244 1 0.6101 0.1256 0.42)7 0. 7790 0.9992 0.147 1 0.0250 0.4742 0.6710 0.10)4 0.2642 0.0217 0.2900 0.5970 0. 1646 0.4 127 0.7411 0.7069 0.1600 0.7190 0.4097 1.01)4 2 I'l'K on Met Hi. Ii.irir 0.4 1 0.1 2 0.1 7 0.14 0.01 0.07 0.17 O.li 0.16 0.11 0.16 0.06 11.7 5.94 10.1 14.1 0.62 21.5 14.2 11 1 27.9 11.6 0. 101 0 , 7 09 5 0,55110 0.2411 0.7442 0.4/4 7 ft.5020 0.4591 1-47 ) 9.6 27 . 4 ) 1.7 1.97 6.01 1 1. 1 12.7 I.ii.id Me t >|h t (J> 0.0940 0.27H0 0. 1240 0.1220 0.2764 0.1194 0.1640 0.1604 0.2714 0.1996 0.1172 0.0044 0.6971 0.0252 0.4)44 0. 11)2 0.2 700 0.0244 U. 1 4 1 0.76/0 0.0444 0.051.0 0.2000 0 2 J 2 0.1900 I'CM oil Li |> id Wt Haul r lvi/l) 11 0. 12 0.45 0.40 0.00 0.22 0.16 0.25 0.1) 0.45 0.53 0.21 11.1 701 19.2 k*P`.d . mat Ni-l wt. * 1 * m 19 ) ) 29 15 12 47 44 40 30 31 12 05 44.9 79 ft 5 ft. 4 19. 7 57 14. 1 37. 1 10.7 . 7.4 50 47 15 21 42 40 42 HONS 0 0 8 5 0 1 TABLE 17. PCD CONTENT OF ADULT SKIN, OMENTUM, AND FAT SAMPLES SHIPPED 24 AUGUST 1978 s oz Sawple 1 .0. Typ* Dltt Shipped Oat* Saiapla Taken Ooa* Level Ing Aroclor 12S4 Met Weight ___Lai____ PPM on Met Mt. Lipid Ilaale Height <pa/al__ ___ ia____ PPM on Lipid Mt. Rani* Ivi/l) Lipid Mt. ____ "MeTTlt. * ,#0% m z H o 701-C f r */24/7* 7/31/71 $14-G M r 0/24/7* 1/2/71 4 2R-J m r */24/7i */2/7l Low Low Low 1.394 1.374 2,414 0.4204 0.4911 0.S11I 2.2$ 3.20 4.21 0.4400 0.3040 0.41)2 3.11 $.11 $. 74 71 42 74 PI 119-M r */24/7* /2/71 Low 1.192 0. 394$ 3.51 0.2700 $.14 4* 432-J m T */24/7* */2/71 Low 333.4 0.7244 1.03 0.2014 42 cos-r l p */24/7* 7/11/71 , 1-ow X n M (4(-l t r */24/7* /1/7I ! Low * J9-N f T 1/24/7* 7/31/71 Low 2.349. 1,049 1.09$ 0.1237 0.$704 0.7441 2.00 1.04 1.47 0.49)2 0.4714 0.4492 1.49 79 3 424-1 C t 0/24/7* 7/31/71 Low 2.442 0.1447 3.00 1.4000 o 742-B 0 */24/7* 7/11/71 Nedlua 473.7 0.3301 2.04 0.0140 1.02 2$ T a> 704-K r 1/24/7* 7/19/71 704-E s */24/7* 7/14/71 High High $47,100 2.221 3.1010 0.170) 172 2.$$ 0.7974 72) 24 o * c 2to Oo OD o hi taihj: in Pen CONTENT OF ADULT FAT AND OHKNTUM r.AMIM.F.S r.llIPPICI) 1 NOVFMOF.R 197fl M O N S A N TO ftC S C A flC H C O R P O R A T IO N Sample I.O. _TyCt Dal e Sli ii'iwd Date Sample TaIpd Dosp l,evel lift Arorlor _____ 1254 Wet We |f|*il .'j!____ PPM on Wt I WL. *.*; i a l.i |>i d We i i|hl 'I'j/uL _____i`j> PPM on Lipid Wt. |)<ms irj/gi Lipid wt.. IAnm Wet Wt. * 1001 (41 *04-1 t 200-r i Slt-H ( 569-11 I 744-e r 274-F t tii-i r 739-f m 426-G * 217-G m 4li-J F F O F F 0 F F F F F 11/1/71 11/1/7* 11/1/74 11/1/74 11/1/7* 11/1/7* 11/1/7* I1/1/7* 11/1/7* 11/1/7* 11/1/7* 10/4/7* 10/4//M 10/4/MI 10/4/?* 10/4/7* 10/4/7* 10/4/7* 10/4/78 10/4/7* 10/4/7* 10/4/7* Control Cnnl roi Conirol Control Control Cunt rol Control Control Control Control Control 353. 3 <337. 1 <281.7 511.4 612.6 257.6 <205.5 735. 3 195.9 400.6 153.2 0. 1191 0.5662 0.4642 0.7260 0.7574 1.4951 2.4073 0.504 I 0.34*6 1.5158 0.9027 0.9) 0.60 '0.60 0. 70 o.ai 0. 17 <0.09 1.46 0.56 0.26 0.17 0.7677 0.0496 0.0420 0.54*0 9.4300 0.5400 2.0700 0.3112 0.0220 1.2456 0.30 32 0.96 <6.81 < 6. 71 0.9) 1 . 42 0.40 <1.00 2. 36 B. 90 0.32 0.40 97 l.l 9.0 75 57 34 4 62 4.3 *2 42 4n-r r 309-1 t 424-1 ( 444-E f 119-11 ( 605-F C 2*4-1 ( 701-C f 514-G m 412-J 119-H m 421-J F F F F F F .F |o F F F F 11/1/7* 11/1/7* 11/1/7* 11/1/7* n/i/7* l1/1/7* 11/1/7* 11/1/7* 11/1/7* 11/1/7* 11/1/7* n/i/7* 10/4/7* 10/4/7* 10/4/7* 10/4/7* 10/4/7* 10/4/1* 10/4/7* 10/4/11 10/4/7* 10/4/7* 10/4/7* 10/4/7* Low Low Low Low Low Low Ixw Low t*ow l*OW la>w Low 1*117 2.322 2.43* 3,587 9,*14 5,702 1,77* 1,221 1,435 59 J. 3 1,321 3,942 0.3620 0.7158 1.0744 0.8906 2.3452 1.740* 0.6471 8.5402 0.4440 0.3543 0.3676 1.0431 3.09 3.24 2.46 4.03 4.19 3.18 2.75 2.26 3.09 1.67 3.59 3.80 0.1540 0.5452 0.9064 0.474* 1.912* 1.4144 0.41*0 0.15*0 0.2020 0.1354 0.228* 0.7*16 7.25 4.11 2.91 5.32 5.1) 4.0) 4.75 7.73 7.0* 4.3* 5.7* 5.07 4) 75 4 74 2 19 45 29 44 31 42 75 757-1 f 27j-i r O F 11/1/71 11/1/7* 10/4/7* 10/5/7* Medium Medium 24,4*0 9.565 0.7332 0.7179 36.1 13.0 0.4596 0.6100 57.6 15.7 76 3-F f 307-1 r 540-11 ( .F 'r if* 11/1/7* 11/1/7* 11/1/7* 11/1/7* 10/5/7* 10/5/7* 10/5/7* 10/5/7* Medium Medium Medium Medium 9,224 18,790 8,806 10,110 0.5*65 0.8971 0.4030 0.45)2 35.7 20.9 21.9 22. 3 0.7040 0. 1940 23.0 76.7 F 11/1/7* 10/4/78 Medium 2,063 0.3*62 5. 34 0. 1100 F 11/1/7* 11/1/7* 10/4/7* 10/4/7* Medium Med 1 urn 5,020 10,060 0.4094 1.2946 12. 3 9.66 D 20-1 F 11/1/7* 10/6/78 Medium 14,730 0.7054 20.9 2 ts> 11/1/7* 11/1/7* 10/5/7* 10/5/18 H i <|h 46,220 10,54(1 0.626* 0.5505 106 55.5 0. 2714 249 30. 3 oo a> 1 1/1/7* n/i/7* 11/1/7* 10/5/1* 10/5/78 10/1 1/1* lll'jh lll'lO Hi |li H 1.1'iO 16,420 M, 2 00 1.50*J 8.SUM Q.19)1 S5.6 71.1 4 1.6 t* o u> 4 2 * - J F O 11/1/7* 11/1/7* 10/6/1* 10/6/ 1* III Mil III'|li 4.0 16 M. 3110 *. 187 1 1. 34/7 7r.* <6.0 4) IS 54 TABLE 19 PCB CONTENT OF ADULT FAT SAMPLES SHIPPED 6 FEBRUARY 1979 1.0. f p aoo-r f tc*-i f 7*4-* f in*: f 2J7- p 2'-r l S7|-M r 7)9-f p <24-0 p p I4(*t f j-r i:**: r. 139-M 5 119-N i 4?5-r * 412-J 7:>i ; 3:*-: # 424-: f 2*4-: t 44-1 |* 7J7-: t 211-C t HM t 54:-H t )! 9 30-: tB S9S-i a 273-1 f 743-t f J07-I f 244-: 437-J a 425-J a m-r f 217-J { 400-1 a 312-1 f TVP* r r r r T r r r r r r r r r r r r r T r r r r r T r T T T t f T F T r r r r r T T Mti Shipped 2/4/79 2/4/7* 2/4/7* 2/4/7* 2/4/7* 2/4/7* 2/4/7* 3/4/7* 2/4/7* 2/4/7* 2/4/7* 2/4/7* 2/4/7* 2/4/7* 3/4/7* 2/4/7* 2/4/7* 2/4/7* 2/4/7* 2/4/7* 2/4/7* 2/4/79 2/4/7* 2/4/79 2/4/7* 2/4/7* 2/4/7* 2/4/7* 2/4/7* 2/4/7* 2/4/7* 3/4/7* 2/4/7* 2/4/7* 2/4/7* 2/4/7* 2/4/7* 3/4/7* 2/4/7* 3/4/7* 2/4/7* Date (apple Taken 13/4/7* 12/4/7* 12/4/7* 12/1/7* 12/5/7* 12/1/7* 12/11/7* 12/7/7* 12/4/7* 12/4/7* 12/7/7* Cnee Level CMtrel Central Control Control Central Central Central Central Central Central Central n* fcroeler 1354 PPn on Met Met vt. Weight Ulil _!i!____ lira/g) 250.4 355.3 <215.3 244.3 <297.* 233.3 <244.1 257.5 <241.* 405.3 <227.4 0.S272 0.5**1 0.4220 0.707* 0.514* 0.1704 0.4715 1.2314 0.3490 0.5534 0.4470 0.30 0.44 <0.51 0.37 <0.50 C. 27 <0.5* 0. 21 <0.77 0.73 <0.49 12/S/7* Lev 12/4/7* Lav 12/11/71 lev 12/7/7* Lev 12/1/7* Lev 12/7/7* lev 12/4/7* lev 12/11/7* lev 12/*/7* Lav 12/7/7* lev 12/5/7* lev 13/4/7* lev 1,170 1.T04 1,014 2,0*4 1.54* 3.454 4.4*9 1.347 434.1 743.3 1.237 1.2*5 0.4144 0.7*34 0.4170 0.795S 0.*124 0.9507 0.9059 o.am 0.2*79 0.3315 0.*371 0.*395 2.73 3.15 2.44 2.43 1.93 3.*5 4.94 1.43 1.51 2.34 1.44 3.01 12/7/7* 12/7/7* 12/11/7* 12/7/7* 12/1/7* 12/7/7* 12/5/7* 12/11/7* 13/4/7* 12/4/7* 12/5/7* Mdla Media Media Media Media Media Media Media Media Media Nadia 5,543 12.590 3,90* 5,033 10.*72 12.410 7,304 17,570 5,431 2,319 24,000 0.M3* 0.4490 0.429* 0.4*2* 0.5571 0.7504 0.2705 0.4047 0.5449 0.41(0 1.3237 4.27 2*. 0 9.09 10.4 19.2 14.5 27.0 29.0 9.93 5.55 l.l 13/4/7* 12/7/7* 12/*/7* 12/4/7* 12/4/7* 12/5/7* 13/7/7* lh Rifta m*h Kifh Uh il*h Mifb 2*,200 14,900 14,040 34,5*0 2S.470 35.390 24.7*0 0.C720 0.4*45 0.4444 0.*751 0.9230 0.7024 0.954* 39.0 21.* 21.1 41.* 30.* 50.4 31.3 pm on Lipid Upld Mt. eignt Peel* <> (Md/dl C.7344 0.4541 0.3792 0.5134 0.4104 0.7*3* 0.42*0 0.4201 0.0444 0.4772 O.OC34 0.34 0.54 <0.57 0.51 <0.43 0.30 <0.42 0.41 <3.1* 0.15 <43.2 0.5400 0.7012 e.371* 0.473* 0.7441 0.7300 0.1124 0.4393 C.2C74 0.294* 0.7540 0.2914 3.34 2.43 3.44 3.11 2.10 5.0* 5.53 2.17 2.09 2.53 1.40 4.41 0.792* 0.3240 0.3*04 0.3*** 0.4414 0.M12 0.2273 0.4(12 0.5104 0.2324 1.17** 4.99 3*. 9 10.3 12.4 31.0 14.2 32.3 39.1 11.0 9.9* 20.4 0.450* 0.4IS3 0.4794 C.1054 0.5414 C.4440 0.4492 5*. 1 30.7 29.3 34.* 50.7 79.7 41.2 Upid at. V.. t 100 1%) 9 7* 0 73 0 90 91 51 24 0.* *2 M 47 5 92 74 90 75 72 90 90 44 90 72 9 3 *3 91 4 74 90 54 9 47 71 74 12 41 43 74 33 MONSANTO RESEARCH CORPORATION HONS 008504 3.:.: ant Rhesus Monkey Skin and Fat Samples Saby Rhesus nonkey tissue samples were taken on an intermittent basis to determine the PC3 levels the monkeys may have accumu lated both during gestation, and after birth as a result of nurs ing. Since the babies were not sampled on a regular basis, presentation c: the data in a cumulative manner would not facili tate interpretation. Therefore, the data entries for the baby m.cr.key tissue samples are given in Tables 20 through 26, in which complete data histories are enumerated. 3.2.3 Liver Siccsv Samples Liver biopsy samples were obtained during the final sacrifice of the Rhesus monkeys in December 1978. Levels of PCB's in the liver tissue were determined to examine whether PCS's accumulate in the liver, an organ that commonly detoxifies the body of many foreign subsoa.-.ces. Table 27 itsos the FC3 levels found in the adult Rhesus monkey liver tissues, and Table 28 gives the PCS values observed in the infant liver tissue samples. 3.2.4 Miscellaneous Samples Analyzed on an Intermittent Basis Several additional samples were analyzed on an intermittent basis. These samples include milk, blood serum, and tissue samples from terminal animals. Ar.v modifications necessary for analysis (i.e., for milk and blood serum samples) are outlined in the section or. "Experimental Procedures." Table 29 lists the PCS content found in fat and muscle tissue of several terminal animals. Tables 30 and 31 present the data obtained for milk samples collected from nursing mothers and one blood serum sample taken from an adult male. Table 32 gives the PC3 values found in the placenta of several female adults. 34 MONSANTO SESEARC-* CORPORATION HONS 008505 TABLE 20. PCB CONTENT OF ABUI.T PLACENTA AMI) BABY SKIN AND FAT SAMPLES .SHIPPED 4 MAY 1970 764-E r MMI -5)1 I MS)) f 1*1521 f p 0 0 0 or 0-0542 r 0 Date 5/4/10 5/4/10 5/4/70 5/4/10 5/4/70 5/4/71 Date Sample Taken Done Level 4/25/70 Cont rol 4/19/70 4/25/70 4/26/70 Cont rol Cnntro) Control 4/25/70 Low 5/2/70 High Int) Aroclor 1254 2.2AO * Wet We i gilt `Ji 4.2315 --- 442.2 1. 465 0.1415 0.1007 We f wt. haata 0.54 -' l.lpl<l Lipid Wt. Weight Ilaala ly) ______ l*3ll 1 0.0144 150 -- -*' L1r!.d_2!i-P im wet wt. m 9.34 -* 3.11 14.6 0.0205 0.0070 21.4 119 14 7.7 `Sample# loat due to contamination problem* encountered. M O N SAN TO R E S E A R C H C O R P O R A TIO N TABLE 21. PCD CONTENT OF BABY SKIN AND FAT SAMPLES SHIPPED 23 MAY 1978 Date Shipped Date Saaipla Taken Dose Level "9 Aroclor 1254 Wet Weight PPM on Wet Wt. Ilaala Lipid Weight i-u/jJ_____ ___ is* PPM on Lipid Wt. Baala 1*4/4) LlEidl... , (tl 0-1574 0-0542 r 0 0 5/2J/10 5/2J/70 5/21/70 5/0/70 5/15/70 5/12/70 Control Control Low 4.405 s. ;oi 31.44 0.1677 0. 1*97 0. 16)5 0.01 0.03 Oil 0.0105 0.0261 0.0350 0.15 0.90 10 21 C 2 i/i 0-0592 r or 5/23/70 5/21/10 5/21/70 5/1/70 5/12/70 5/21/70 Medium Medium Medium 131.9 20.11 51.94 0.2200 0.11)0 0.1111 0.30 0.25 0.47 0.0209 6.30 9.1 O o a> Ui o O' Sapl 1 -0. B-05II r l-ISII B-IS)) i B-0551 m A-9421 r 0-0577 r -404 a -542 1 B-0546 1 -SSI -ISI0 -542 f TM\U: 2 2. ven CONTI ;nt or llAIW S KJN r.AMI'`i.i-::; : niPiT.o \ rt .nmi: I97n Data Shipped Data Sai>lc taken Doan Love 1 Inq roe lor 1254 Met We i ijht I'll 1`1'H on Wet Wt . ft.ia t a Uy/yl I.ipld We i >|ht `Ji A 4/15/21 5/24/7* A 4/15/2B 5/24/11 A 4/15/11 B 4/15/11 5/24/7* 4/4/7B A 4/15/IB 9/9/19 Control Con)ro1 Control Control Control n. l. 24.24 4.14 14.9# 25.50 0.1171 o. mi 0.2)41 0.2 120 0.1414 M.ll. 0.2| 0.02 0.07 0.16 0.0151 0.01)5 0.041) 0.0541 0.042 1 B 4/15/7B B 4/15/11 A 4/15/71 5/24/71 5/1B/7B 4/I/7B IXM laM Lew 14.11 1.12 5.54 0.1 121 o. mi 0.2114 0.1 ) 0.04 0.0) 0.0105 0.0095 0.050) B 4/15/IB 5/3B/1B B 4/15/7B 4/5/71 B 4/1 5/IB 4/9/70 B 4/15/li 4/9/7# Medium Medium Medium Medium It) 4)1.2 199.1 172.1 0.1500 0.1412 0.095# 0.1201 0.55 2.49 2.09 1.44 0.004) 0.017) 0.014) 0.005) B 4/15/7B 4/5/7B lllqh 271.2 0.1510 1.12 0.0045 P(`N on Liptd wt. ``I'*!*. l.uYlJ_____ n. d. 1.10 - 0.10 0.11 0.40 . loot It) 11 11 IB 71 24 0.55 0.40 0.12 21 24 19. ) 25.0 14.0 )2.9 2.9 11 15 41 . 4 ).0 TABLE 23. PCB CONTENT OF BABY SKIN AND FAT SAMPLES SHIPPED 24 AUGUST 197B SMpI* 1.0, -til f Msn r A-isjj r | 0-05)2 f 1 fl-0510 a H-0510 a B-0604 a B-0527 1 B-0527 ( A-0511 a M 051 l a H-0401 A 0517 1 Date Shipped B/24/1B 0/24/7# 0/24/70 0/24/7# I/24/7B I/74/7B 9/24/19 9/24/19 B/24/7# 9/24/19 9/24/19 4/24/19 9/24/19 9/24/14 Date Sample Taken 4/24/71 4/24/7* 4/24/7# 4/24/7B 4/19/7# 4/19/7# 4/27/7# 4/21/7# 6/2J/7N 6/19/70 6/11/1* 6/77/)* 6/21/iH 6/ 10/7* none Leva 1 Iliq Aroelor 1254 Cont rol Control Cunt rol Control Control Cont rot 1, 4B0 419.6 942.4 1 J4.I 917.4 2 20.0 (aim Iaiv )0I .4 1.002 2, 114 lieil i um Metl i im Ned i oat Ned 1 um 72)4 0 0 1 . 1*1 1. 10/ II f|l. 2. it'.* Wet We 1 cjht <n 1.4)00 0.9700 0.4125 0.4052 1.0404 0.4909 0.2100 0.007) 0.5001 0.71#) 0.1154 0.2001 O 1671 0 1416 We* Wt. Iiaala 1.41 0.90 0.44 1.11 4.6) ill M. * Lipid Welqht 0.0544 0.7764 0.0 If.0 0.07 12 Pl'M on Lipia wt. Masts __tMy/q)__ I . ) l.H 2.44 1.4) I SO 4.04 wt. * ____________ (I) 10.1 ). 12 . J 45 55 141 1.4) 44. I It* . 2 R. I OS BOO SNOWI TABLE 24 PCD CONTENT OF BABY SKIN AND FAT SAMPLES SHIPPED 24 AUGUST 197B M O N S A N TO R E S E A R C H C O R P O R A TIO N I .0. Data Shipped Data Staple taken Deie Level 9nq hrocinr 1254 Wet Weight __ LaJ___ PPM on Wet Mt. Rattle li.Vgl PPM on Lipid Li pi it Mt. Weight latli _tj}i______ ipq/qi Lipid t. Wet Mt. * 1001 _______ m -514 -514 -557 -557 Mt -421 f r ( */24/9* */24/7* /J4/7* */24/7* /24/1* 7/U/1* 7/27/7* V4/1* 7/4/7* 7/10/7* Control Control Control Control Control 04*. 4 52*. <147.2 392.4 <241.2 0.4519 0.5410 0.2994 0.7307 0.2050 <0.77 0.92 <0.54 0.54 <1.30 0.0)74 0.2234 0.01)2 0.SI 40 0.0720 <9.27 2.U <2.20 4.70 0.71 i.) 40 24 10 )S -404 /24/7I 4/24/70 -542 r 4/24/7B Ui -542 ( nr /24/70 7/27/1* 7/27/1* 7/10/7* 7/10/7* Low Low 1ow Low 1.124 *.734 14.740 0.4951 1.1*09 1.42 . 40 0.0740 0.S4I4 0.0700 0.7112 IS.2 IS.4 IS.5 20.7 11 S2)I 60 -544 t 4/24/1* n-1544 r n 1/24/74 -544 r of 1/24/7* -40) 8 1/24/7* B-140 J Bf 4/24/7* 1559 B /24/n nr /2 4/70 rB / 2 4/7# /24/7* -540 r n /24/7 -542 r n 4/24/7* 7/5/71 1/2*/74 7/2B/7I 7/27/7* 7/27/7* 1/10/7* 7/10/7* 7/25/11 7/25/74 1/10/7* 7/)1/7* Medium Med ii<a Medium Hfdlua Medium Med um Medium Medium Medium Medium High 1. 394 30,410 17,774 72,440 14,150 60.540 17,720 97. 310 1,270 4, 397 0.2010 0. 1410 0.4*95 0.4512 0.5905 4.94 )0.0 0.040) 109 0.0114 0.0244 0.04S2 0.0)24 0.2)70 0.1424 0.4944 0.2042 1.1714 0.0012 0.0000 171 Ml 444 549 )!) 99.4 07.2 44.7 l).0 1.051 5.494 5.1 )9 6.6 SI 25 70 )5 6) 5.1 2.0 HONS 0 0 8 5 0 8 l MONSANTO RESEARCH C O BORAT TABLE 25. PCD CONTENT OF ADULT SKIN AMD FAT SAMPLES AND DADY SKIN AND FAT SAMPLES SHIPPED 1 NOVEMHEH 1970 Sample Date I .0. Tyre Shi |ii>c<l 8'1(21 1 B -1(21 1 of 11/1/78 11/1/7# B-87S4 8-8754 B-8754 B-8754 ( f 1 f A B B( 11/1/78 1 1/1/71 11/1/71 11/1/7* B-8510 ( 8-8540 i B-8540 ( B 0 B 1/1/71 11/1/7* M/I/71 B-IS42 Bf 11/1/71 Ml-ll f Mi-ti r S r 11/1/7* 11/1/2# nt-i r F 11/1/78 Date Saawde Taken //78 8/8/78 8/10/78 8/11/78 10/10/78 10/10/78 8/10/78 8/11/78 10/10/78 I/V78 i/11/78 8/11/78 8/21/78 Doan Leve 1 nq Aroclor 1254 Control Control 244.2 71*.2 Low l.nw |,nw Low 2,410 1,484 1,154 11,280 Medium Wedltua Medium 8.512 2,748 550.2 Hl<|h 4,128 Med 1 urn HOiiluM Ml <|U 1. 257 28,000 84,00 wet Wi<fht _<a! 0. 1810 1 . 11 J5 0.1708 0.2101 0.2818 1.2588 0.0882 ft. mi 0.1882 0. 1052 0.4807 2.84 77 0.8202 PPM on Hot Wt. iiirl a 0.45 0.41 14.2 5.48 4. in 8.04 84.0 8.79 2.91 19.2 4 . *4 1 1.7 101 Lipid We ijht MJ* 0. 1244 1.0740 PPM on Lipid Mt. Baal a Iu9/ll 1.88 0.74 0.0454 0.0484 0.07)2 0.7480 5 1. 2 21. 2 14.8 14.1 0.0020 0.0)82 0.00(0 4, 270 1 44 81.7 o.ooot 0.0484 0.1028 5. 160 47. 7 48.6 0.0404 1.401 MLiept idWl-- `a 1001 m 12 2 27 26 77 41 2.0 4. 1 1.2 0.1 7.2 28 7.4 TAI1I.R 26. PCD CONTENT OF DADY SKIN AND FAT S AMPLES SHIPPED (, FEBRUARY 1979 Sample I.D. Date Type Shipped Date Sample Taken Dose Level Inq Aroelot 1254 Wet w* i <jli t 9 PPM on Wet Wt . Basis Lipid Wclqht (119/91 _______l3> PPM on Lipid wt. (las is (uq/'i) Lipid Wt. Wet Wt. x 100 [\) n-oois 0 2/6/70 10/27/70 ls>w <232.1 0.1194 < 1.94 0.0104 <12.6 15 n-uei5 u 2/6/79 11/27/711 ) <ow <272.6 0.2270 <1.20 0.0736 < ). 70 32 X B-8B15 or 2/6/79 12/6/70 Low 333.1 0.4389 0.76 0.3492 0.95 00 5 z to 11-0060 m ii 2/6/79 11/10/70 Med i um 1,053 0.00)1 12.7 O.0060 175 7.2 11-0060 m n 2/6/79 12/11/70 Medium 4,623 0.2914 15.9 0.0956 40.4 3) o o 11-11560 f n 2/6/79 11/9/711 Med i tun 1,2)11 0.1790 6.00 0.0104 67 . 1 10 O) H-0560 r nr 2/6/79 12/11/70 Med Hun 7,990 0.4423 111.1 O.1400 57. 1 12 OsO TABLE 27. PCB CONTENT OF ADULT LIVER SAMPLES SHIPPED 7 MARCH 1979 J.C. Tva Date Shipped Date Saeplt Taftan Daaa Laval nf Aroclor 1254 Mat Maight U> PPM on Mat Mt. Util Lipid Maifht PPM on lipid Mt. Saili -<*___ ___'"a/ii___ Lipid wt. Mat Mt. a JOO <%> m 2U-T f 221-C m 7)9-f m 1M-C t die-1 f f :co-r r JIM- ' *:<: f 414-J r L L L 1 L L 1 1 L 1 L 1/7/74 3/7/74 3/7/7* 3/7/7* 3/7/7* 1/7/7* 3/7/7* 3/7/7* 3/7/7* 1/7/7* 3/7/7* 13/7/74 12/7/74 12/11/7* 12/7/7* 13/5/74 12/4/7* 12/4/74 12/4/74 12/4/74 12/4/7* 12/5/7* Control Control Control Control Control Control Control Control Control Centrex Control >41.7 443.* 2,311 449.4 33.95 49.24 4*. 44 1.014 44.97 44.41 224.0 3.4094 S.0S14 3.3934 1.4402 1.40*0 1.7371 3.2057 3.5207 2.0941 3.0*42 1.71*3 0.10 0.09 0.70 0.24 0.03 0.03 0.02 0.29 0.02 0.02 0.13 0.1540 0.2524 0.2730 0.0454 0.0392 0.0429 0.0432 0.0794 0.0574 0.0740 0.04*4 0.94 1.74 *.75 9.44 1.34 1.17 C.77 12.7 0.42 0.44 4.59 10 5.0 9.0 2.5 2.4 2.4 2.0 2.3 2.1 3.5 2.1 !16-3 . 'ci* r f j:-i f - - <r3-.r f fc*4- i:9-w it. 4J9-P ; :s-r t 1 L 1 L m 1 1 1 1 - 1/7/7* 3/7/7* J/7/7* 3/7/7* 3 '7/7* 3 '7/7* 1/7/7* 3/7/7* 3/7/7* 3/7/7* 3/7/7* 3/7/7* 12/7/7* 12/4/7* 12/7/7* 12/5/7* 12/5/7* 12/11/74 12/11/7* 12/4/7* 12/7/7* 12/1/7* 12/4/7* 12/4/7* Lev Lev low Low Lev low low Lew Low low low Low 290.0 20*. 4 309.4 212.3 471.1 337.3 471.2 437.2 3.017 1.493 2.291 1.520 0.55*0 0.5097 0.7773 0.7100 1.40*8 0.7444 0.4035 0.9414 4.4045 5.0535 5.7247 4.1997 0.52 0.41 C. 42 C. 30 0.33 0.45 0.7* C. 44 0.44 C. 30 C. 40 0.22 0.01*4 0.0234 0.C332 0.0300 0.0776 0.0552 0.0320 C.0392 0.2272 C.2794 0.1344 0.1494 15.8 *< 9.31 7.04 4.07 4.11 14.7 11.2 13.3 5.34 14.7 10.2 3.3 4.4 4.3 4.2 5.5 7.4 5.3 4.2 3.4 5.5 2.4 2.2 3'J-X i J55-I t 23-1 1 S45-* * 'H f 44-1 & 595-1 241-C ( 307-1 ( 7)*I f 7S7-I r 403-1 * 42S-J 344-1 t 217-1 l 224-r t 312-1 t 437-2 L L 1 L 1 t 1 1 1 1 L 1 L 1 L L L 3/7/7* 3/7/7* 3/7/7* 3/7/7* 3/7/7* 3/7/7* 3/7/7* 3/7/7* 3/7/7* 3/7/7* 3/7/7* 3/7/7* 3/7/7* 3/7/7* 3/7/7* 3/7/7* 3/7/7* 3/7/7* 12/4/7* 12/7/7* 12/5/7* 12/*/7* 12/4/7* 12/7/7* 12/11/7* 12/11/7* 12/5/7* 12/7/7* 12/7/7* Madivfti Madim Hadiia Madiiss Mad itot Had1 tan Madita Madina Madtuft Madivs Modiwo 2,174 2.159 4,424 5,730 5,507 2.994 491.0 593.4 14.730 900.5 1,434 1.5*41 1.4454 1.1*50 4.7500 4.3*17 4.*0*7 4.2944 4.9419 5.3315 1.5024 2.0240 12/5/7* 12/*/7* 12/4/7* 12/4/7* 12/5/7* 12/7/7* 12/7/7* Nifh Bifh i*h Bifh ifh Mifh lifh 19,340 5,410 14,150 15,230 44,010 17,470 -- 704.1 3.4173 5.39*4 5.9440 4.*0*1 4.4220 5.5443 2.0254 1.11 1.55 3.73 1.21 0.44 0.42 0.11 0.09 2.7* 0.49 0.91 5.07 1.04 2.71 3.17 9.94 3.19 0.35 0.038* 0.07*4 0.0592 C.110* 0.2C74 0.1*00 0.144* 0.2760 0.3500 0.0420 0.C500 74.1 34.5 74.7 51.7 24.5 14.4 4.71 2.11 42.1 14.5 34.7 0.1348 0.1134 0.14*4 0.17*0 0.1474 rl412 0.042* 134 49.4 95.9 *5.4 312 92.4 *.53 2.4 4.2 5.0 33 3.2 3.7 2.3 4.0 4.4 4.1 2.5 3.7 2.1 2.1 3.7 3.2 3.4 4.1 39 MONSANTO RESEARCH CORPORA! ION a HONS 009510 h TAM.E 20 pen content op iiahy liver sample: shipped 7 march 1979 Sample J.D. Typo Date Shipped Date Sampl e Taken Dost* Ia*ve l D-aeis ni 1/7/79 12/6/78 Low B-0860 B-8560 m 1/7/79 12/11/10 Mnl i urn Ml 1/7/79 12/11/711 Medium Inq Amdoi 1254 273.7 Hot Woirjhi <yi PPM on Wet ML. Li f*i<l n.nis Wo iqht (p'l/'ll ___ L9> PPM on 1.11> 1(1 wt. has i s I UM/91 3.5904 o.on 0.1400 1.95 Lipid wtwt wt. x 100 It) 3.9 3,093 1,233 1.2517 i.sum 2.47 0.70 0.0216 0.0344 131 75.0 1.9 2.2 TABLE 29 pen CONTENT OF BABY AND ADULT FAT AND MUSCLE TISSUE OF TERMINAL ANIMALS SHIPPED 18 APRIL 1978 i .0. n-MU ( It-Mil ( ii-r t 414-H 1 770-1 1 Tyi*c. II* Ilf r Dm * Sli 4/lb//I 4/10/70 4/10/71 r 4/10/70 r 4/1R/78 Dtr f>at>lc Taken 1/3 4/70 J/74/0 1/11/70 Inq _ 1 2 4Hiini! moo lor S Cunt r<l S14.I Coni fu1 < 4 S 7 .; Coni ini < ill.; 1/23/70 4/1/10 Itfllt llf(lt t. 440 140.700 Wot W<* i jhl 1 4 0. r,H 0. 4 1117. 1.4410 I). 7442 2.3401 PPM on 1W-l HI. It Ail s hfi/oi 0.40 < 1.04 <0.14 4 . 47 142 l.ipitt Muitjht l*l> 0.01*8 0.0044 1 . 1440 0.02 74 Q. 7140 PPM on 1 8 Mt . Il.l t 6 3.4 <71.4 <0.27 214 474 m 1 .0 i *> 70 1.0 12 HONS 0 0 8 5 1 1 TABLE 30 PCB CONTENT OF MILK SAMPLES SHIPPED 2 MAY AND 23 MAY 1978 Sample I .D. 404-1 618-1 764 - E 27 4 -F f f f f 624-1 f 540-H f 273-1 f 757-1 f 312-1 f Type M K K K K K M M M Date Shipped 5/2/78 5/23/58 5/23/78 5/23/78 5/23/78 5/23/78 5/23/78 S/23/78 5/23/78 Dose Level Control Control Control Control Low Medium Medium Medium High ng Aroclor 1254 8.0 0.6 ....' 3.1 21.1 22.5 43.6 60.9 49.0 100.1 Weight of Milk Analyzed to) 0.0992 0.1039 0.1041 0.1075 0.1037 0.1048 0.1063 0.1074 0.1055 PPM on Weight Satis (uq/o) 0.08 0.01 0.03 0.20 0.22 0.42 0.51 0.46 0.95 Saapl. I.D. 605-r f 200-F f 701-E l 483-F f 763-E i 600-1 m TABLE 31. PCB CONTENT OF MILK AND BLOOD SERUM SAMPLES SKIPPED 7 MARCH 1979 Type Date Shipped Date Staple Taken Dose Level ng Aroclor 1254 Weight oj Staple Analyzed (9) PPM OR Weight Basis (U9/9) M 3/7/79 5/9/78 Control <1.1 M 3/7/79 6/8/78 Control 3.1 0.0622 0.1006 <0.018 0.031 M 3/7/79 5/27/78 Low K 3/7/79 9/28/78 Low <1.1 i.i 0.1028 0.1029 <0.010 0.011 K 3/7/79 5/23/78 Medium 44.3 0.0984 0. 450 S* 3/7/79 9/27/78 High <1.2 0.0998 <0.012 41 . MONSANTO RESEARCH CORPORATION MOMS 008512 TABLE 32. PCD CONTENT OF PLACENTA SAMPLES SHIPPED 23 MAY 1978 .M O N S A N T O N M E A N C M C O R O N A T IO N Sanple Date 571-H r P 5/23/7* low f 757-1 t 271-1 t 7()-E 1 P P P P 5/21/71 5/21/7* 5/21/7* 5/21/7* n.d. not detected Rate 5/15/7* 5/*/7* 5/3/7* 5/9/7* 5/21/7* Done Level log Wet Arm: lor Wi. t|ht 1254 ____ !Sl Control 17.14 3.24(4 Low n.d. 14.2450 Meritu Moil i im Medio* 14.(2 2 J. #* 22.51; 1 (.4)75 7.1219 5.4291 PPM on Mot Wl. Paata lvg/ql 0.0053 n.d. 0.0024 4.0034 *.0042 1.1 f> iri Weight _Ja!_____ 0.017* 0.0144 0.0374 0.0)40 0.0244 PPM on Liotd Wt. n*ata 01/31____ 0.97 n.d. 0.44 0.70 0.44 l.t_pid wt. wet Wt. 14) 0.54 0.0* 0.5* 0.47 0.44 HONS OOB513 APPENDIX A ANALYSIS OF CORN OIL FOR AROCLOR 1254 Several individual batches of corn oil were spiked with Aroclor 1254 by MIC in St. Louis, Missouri. Each batch was analyzed for PCS content prior to shipment to Litton Bionetics for dosing. Litton then returned any unused portions of corn oil to MIC for subsequent reanalysis. MIC analyzed the corn oil by dilution with Nanograde# hexanes followed by analysis by electron capture gas chromatography. Dilution factors used for the analysis of the oils are 1:100 for T III, 1:25 for T II, and 1:10 for T I and the controls. Results of the analyses are presented in Table A-l. 43 HONS 008514 TABLE A-1. LEVELS OF AROCLOR 1254 FOR CORN OIL (ppr.) Date 2/25/77* Feed Code T III T II TI Analysis of Analysis of Spiked Oil Returned Oil (ppm) (ppm) 219.2 46.7 n.i 215.4 52.9 11.9 Month re- April 1977 May 1977 A / Zz ~ 7 III T II 7I 199.1 52.5 10.7 t June 1977 a 6/25, T III 7 II TT Control 211 46.7 9.2 <0.6 214 45.4 12.0 0.1 July 1977 August 1977 7 '*s *7 7 T III 7 II 7z Control 236 - 49 11.1 1.1 245.5 1 12.8 a September 197 5 '24/77 9/27/77 7 III 7 II t: Control m T~- 7 4a 4 Control Ar.alvsis Data Lost 218 55. 7 12.5 0.1 192.9 54.9 0.6 207.0 49.6 0.2 October 15~7 November 157) 10/27/77 T III T II 7I Control 209.4 46.4 9.8 0.6 228.8 56.4 10.2 0.2 December 197' 11/21/77 T III T II TI Control 179.2 46.6 13.0 0.1 154.4 36.5 9.8 <0.1 January 1978 12/20/77 T III T II TI Control 180.3 65.5 10.3 <0.2 178.0 45.7 8.8 February 137 44 MONSANTO HESEAWCh (continued: HONS 008515 Date 1/25/78 2/24/7B 3/22/78 4 '20/7 S 5/25/78 TABLE A-l (continued) Feed Code Analysis of Spiked Oil (ppm) Analysis Returned 1 (com) T III T IX TI Control 201.5 60.1 14.5 0.2 196 60.6 17.1 0.9 T III T II TI Control 161 48.3 9.6 <0.7 176 46.5 10.5 0.1 T III T II TI Control 180 61 14.7 0.2 226.0 56.0 13.0 0.3 T HI 7 II -a 7 ::i T II 7I Control 195.1 3.8 16.5 196 50.1 12.1 0.2 189 59.1 11.8 t r t t Double batch prepared Batch completely used up; none r eturned for tBatch returned unused h Fed March 1978 Aoril 1973 45 MONSANTO OESEAWCH CO=Oa*TlON MOMS 008516 APPENDIX B ANALYSIS OF RALSTON PURINA MONKEY CHOW Three individual portions of monkey chow, fed as a daily dietarystable, were analyzed to determine any background ?C3 levels the monkeys may have received. The analysis methodology is delineated in the "Experimental Procedures" section of this report. Values obtained fcr PCS's in the monkey chow are given in Table 3-1. In ail three samples, the PCB level was less than Q.C5 ug ?C3 par era- of monkey chow. T.A3LE o-I. AROCLOR 1254 ANALYSIS Or RALSTON PURINA MONKEY CHOW SAMPLES t'jO S&r.ple I. D______Sanple Weight (g) Aroclor 12 54 ,,? ?C3 'z key Chew i .key Chow- *2 key Chow *3 10.0804 10.0042 10.0196 <0.50 <0.50 <0.50 <0.05 <0.03 <0.05 46 MONSANTO RESEARCH COR^ORAT-Os HONS 008517 APPENDIX C STATISTICAL ANALYSIS OF PCB CONTENT OF MONKEY TISSUE 47 HONS 008518 Statistical Analysis of PCB Content of Monkey Tissue IHTROPUCTIOK This report covers the statistical analysis of analytical data collected from a subacute feeding study on the concentrative effects of polychlori nated biphenyls on adult Rhesus monkeys and their offspring. (Report MDA-041, "PCB Content of Monkey Tissue" - Beverly J. Warner, 7 May 1975). The following description of the study is the introduction from that report. "This study is designed to determine the concentrative effects of poly chlorinated biphenyl (PCB) compounds in a subacute feeding program in volving forty-eight adult Rhesus monkeys and their subsequent offspring. The Rhesus monkeys were fed Aroclor 1254 (a PCB compound composed of 12 carbon atoms and 54% chlorine per PCB molecule) as a supplement to their diet. The feeding study was conducted by Litton Bionetics, Kensington, Maryland, for a period of one year. Fat tissue biopsies of the adult animals were taken by the Litton Laboratory and shipped to MP.C for the analysis of PCB content periodically during the feeding study (approxi mately every two months) and during an eight-month relaxation period following the feeding study. In addition to the adult fat samples analyzed, numerous infant skin and fat samples, and colostrum (milk), placenta, and blood serum samples were analyzed on an intermittent basis. At the termination of the project, liver biopsies were also provided for all adult animals and several infants. In all, in excess of seven hundred individual analyses were performed for PCB by various extraction techniques followed by electron capture gas chromatography (EC-GC) . The analysis techni ques utilized and the data accumulated are presented in the following sections of this report." The statistical analysis centers primarily on the adult fat and adult liver samples which had relatively complete and well-balanced data. No samples were excluded. Data were not complete for the baby skin, baby fat and milk samples, but these are presented for inspection, with statistical analyses where applicable. All the data treated were assumed to be log-normally distributed, and averages and confidence limits were calculated on this basis and converted back to the original scale. SUMMARY OF RESULTS Adult Fat Samples (male and female) PCB level in adult fat samples for animals fed the highest amount of PCE is significantly higher than control from one month through 20 months. Average PCB in tissues for this period is 28.2 PPM (wet weight basis) and 53.5 PPM (lipid weight basis). 48 HONS 008519 S-81-SS-Z Page 2 PCB level in adult fat samples for animals fed the medium level of PCB is significantly higher than control from 4 months through 20 months. Average PCB in tissues for this period is 13.2 PPM (wet weight basis) and 20.5 PPM (lipid weight basis). PCB level in adult fat samples for animals fed the low level of PCB is significantly higher than control from 7 months through 20 months. Average PCB in tissues for this period is 2.8 PPM (wet weight basis) and 4.2 PPM (1 ipid weight basis). PCB level in adult fat samples for control animals (no PCB fed) averaged 0.30 PPM (wet weight basis) and 0.55 PPM (lipid weight basis) for the period from 1 to 20 months. Adult Liver Samples - 20 Months (male and female) PCB level in adult liver samples increased in proportion to the amount fed. On a wet weight basis, the control samples averaged.QJ176 PPM, low level samples averaged 0.4 PPM, medium level samples 0.81 PFM and high level samples 2.43 PPM. On a lipid weight basis, these results were 2.2 ppm, 10 PPM, 23 PPM and 75 PPM respectively. Eaby Skin Samol es PCB levels found in baby skin samples Increased with increasing PCB in parents' diet, and with sampling time up to 7/31/78 (insufficient data for statistical analyses). Baby Fat Samples PCB levels found in baby fat samples increased with increasing PCS in parents' diet, and with sampling time up to 7/31/78 (insufficient data for statistical analysis). Milk Samples There was a significant increase of PCBs in milk as a function of the PCB level in the feed. The data were too widely scattered to determine what kind of model best fit the data. The linear model gave the following equation: Amt. PCB in milk 0.094 + 0.0097 (amt. PCB fed). The correlation coefficient was 0.92, significant at 99.91 P<O.OC1. DESCRIPTION OF STATISTICAL APPROACH Adult Fat Samples - Wet Height and Lipid Height Basis Table I shows the output from a computer program which calculates the 951 confidence limits of the mean of the amount PCB found in the adult fat samples. The program converts the data to logarithms, calculates the mean 49 HONS 008520 S-81-SS-2 Page 3 and standard deviation of PCB found for each level fed at each sampling time. then calculates 95% confidence limits of the means of the logarithms. This information is converted back to PPM, and 95% confidence limits of the mean are reported in PPM and as percent of the mean. It Table II is a summary of the averages by month for each level of PCB fed. Table III gives specific statistical conclusions by month. Figures la and lb are composite plots of the means and 95% confidence limits of PCB found for each level fed vs. sampling time. The test for significance was based on the degree to which the 95% confidence limits of the means overlapped. The least significant difference (95% level) between the means is approximately 2.828 standard deviations. When there is no overlap of the 95% confidence limits, the means are approximately 4 standard deviations apart. The individual comparisons can be confirmed using Students' t test. Adult Liver Samples - End of Study The procedure for calculating confidence limits of the means is the sane as in Adult rat Samples. Figures 2a and 2b show the means and confidence limits of the means for PCEs found at each level of PCB fed. Least squares lines were determined for the average PCEs found vs. amount PCB fed. For lipid weight samples PPM found (lipid) = 4.6 + 0.71 (PPM in feed). For wet weight samples PPM found (wet) = 0.20 + 0.022 (PPM in feed). Both these equations were significant at 99.9% (p<0.001) Baby Skin Samples Table IV shows average PPM found on wet weight basis in baby skin as related to level fed to parents, and sampling time. Lipid weight results are not shown because of extreme variation in lipid weight to sample weight ratio. Babv Fat Samples Table IV shows average PPM on wet basis, presented as indicated in paragraph under Adult Fat Samples. Milk Samples Table V shows the average PPM found in milk samples, presented as related to PCBs consumed. 50 MONS 008521 S-81-SS-2 Page < DISCUSSION The foregoing analysis of the data is an attempt to describe the effects of various levels of PCB in the diet on the resulting amount of PCB found in the animal tissues. No attempt was made to fit any sort, of theoretical model, but the adult fat data may be sufficiently consistent to support such an approach. In addition, we have not tried to relate PCB levels to toxicology data, but recommend that this be done when the latter are avai1 able. ACKNOWCCDSNEM The assistance of J. L. Davis (Environmental Sciences) in running the computer calculations of means and confidence limits is gratefully appreciated. 51 HONS 008522 TABLE I Calculation of Confidence limits M* H 7CO9**nT*O4 l WT t2 SICA> CONFIDENCE 'INITS Ar LIMITS AS X OF MEAN ARE 76 49,- S-BMS-Z Page S TftKE 6 ton WET hf4M . 495098 9Q5. <? S1CMA) CONFIDENCE LIMITS ARE 3S34 Tn 693611 9SX LIMITS AS X OF MEAN ARE 71.3797 X TO 140 09b X TAKE 6 MEDIUM WET MAN .56504? 9SZ (2 SIGMA) CONFIDENCE 95/. LIMITS AS X OF MEAN t TAKE 6 MICH UFT 6882*5 SIGMA) C 30336 X 6 controi lipid m* an t 71628 5/. (2 SIGMA) CONFIDENCE LIMITS arc 361069 TO B lSPO 95'. LIMITS AS X OF MEAN ARE 21 0378 X TO 47S.33S X TAKE 6 LOU LIPIP 7 6 31S 9S7. <? SIGMA) CONFIDENCE LIMITS AR .471052 TO l. 13428 957. LIMITS AS X OF MEAN ARs 61 6296 X TO 161 735 X take MEAN MEDIUM LIPID 91S273 X (2 STCMA) CONFIDENCE LIMITS 9SX LIMITS AS X OF MEAN ARE S7 1 59674 X TAKE 6 HIGH LXPIO nfan 9S32S5 ASX <2 SIGMA) CONFIDENCE LIMITS ARE 44339* TO 9SX LIMITS AS X OF MEAN ARE 46.5116 X TO 215 X 2 .0495 TAKE 7 CONTROL WET mean - .235699 S7. <? SIGMA) CONFIDENCE LIMITS ARF. 9SX LIMITS AS X OF MEAN ARE 46 273 1091S7 TO S09798 TO 216 109 X TAKE 7 LOW WET MEAN .422009 9X (2 S1CMA) CONFIDENCE LIMITS ARE 233391 Ti 763059 9SX LIMITS AS X OF MEAN ARE SS.3049 X TQ ISO. I 16 Z TAKE 7 MEDIUM wet f*F an i 23347 5X <2 SICMA) CONFIDENCE LIMITS ABF 9S7 LIMITS AS X OF MEAN ARE 37.378? TAKE 7 HIGH UFT MEAN 10.9164 ASX <2 SICMA) CONfIDgNCE lIHITS 9SX LIMITS AS X OF MbAN AR 70 be 7.71151 TO 15 4S3* 417 X TO 141 559 X 52 MONS 008523 Tftj>tE 7 CONTROL LIPID hCAN 397814 957 <2 SIGMA) CONFIDENCE LIMITS ARP 140902 TO 1 0674 957 LIMITS AS X OF hEAN ARE 36 332S X TO 275 236 X a?l 7 LOU LIA I (r AN S34Q? -S:; (2 SIGMA) CONFIDENCE LIMITS aRF V. LIMITS AS 7. OF MEAN ARE 54.2442 289713 TO 98460 : TO 104.351 TAKE 7 MEDIUM LIPID 2 13993 ,,?J2 S-BMS-Z Pij 6 TAKE 7 MI CM L IP ID man 14 1806 957 (Z SIGMA) CONFIDENCE I.IMITS ARE 6 8624 TO 29 3C3 9S>. LIMITS A3 X OF MEAN ARE 48.3929 2 TO 206 642 % T?iE e COnT*Qi. WET mean - 515025 9*7 <2 5ICMA1 CONFIDENCE LIMITS APE 30C366 TO 697355 95/. Llrirs A* Z O' MtAN ARE 73 654 X TO 135 402 X Tiv, r9.Rr 2A9S-; A CONTROL lIPID > 654*61 ? 5ICMA) CONFIDENCE VrIMITS ARE IrtITS As X OF MEAN ARE 70 029 ^934557. TAKE 9 CONTROL NET H-AN 801472 *57. <2 R1C.MA) CONFIDENCE LIMITS AF 5996 TO l 29722 ?SX LIMITS AS X OF MEAN are 67 951 X To 147 165 X TAKE 9 LOW WET MEAN * 964053 957. (2 SIGMA) CONFIDENCE I.IMITS 95X LIMITS AS X OF MEAN ARE 57 1 68950 X TAKE 9 MEDIUM WET ME on 5 99341 957. (2 SIGMA) CONFIDENCE LIMITS ARF 4.0US8 TO 8.9S433 9SX LIMITS AS Z OF MEAN ARE 66 9331 X TO 149.403 X TA8LC 9 HIGH UET HFan 20 0999 9SX (2 SIGMA) CONFIDENCE LIMITS ARE 14 3207 TO 20.2113 9SX LIMITS AS X OF MEAN ARE 71.2475 X TO 140 356 X M9T5EAMAKNE(2 * S1 CIG2OM3N9TAR9) Oj LCOLNIFPIDIDENCE Wn 957 LIMITS AS X OF MEAN ARE TO 1 07713 take 9 c.Ou lipid __ _ _ 1IMITS Apr 95*96 TO 4 04372 957 LIMITS AS X OF MEAN ARE 4 5936 X TO 205 708 X 53 HUNS 0085i4 TAKE 9 MEDIUM LlPID hC*M > 7*0867 o'/. <2 SICnA) confidence limits a*f 6StS to t2 4450 952 LIMITS AS 2 OF MEAN ARE 61.1143 X TO 163 574 7. S-8KSS Fiji 7 TAKE HIGH LIPID MEAN - 3? 033? ?SZ (2 SIGMA) CONFIDENCE I 1H1TS ARE 21.8312 TO 47.0DS 952 omits AS x OF MEAN ARE 66 1501 2 TO J 46 73S X TAKE 10 CONTROL WET HFAN 24?S?4 9SX 12 SICnA) CONFIDENCE lIHJTS ARF 0792726 TO 7733tS ?S2 Linns AS 2 OF MEAN ARE 32 0172 X TG 3l2 332 X TAKE 10 LOU WET neAN - . *4096? 9*2 (2 SICNA) CONFIDENCE LIHJTS ARE .180674 TO 1.07627 957. LiniTS AS X OF WEAN are 40 972 X TO 244 36* 2 TAf-.S 10 nFDI'JM WET h = i.N a 7 4SPS2 */ (2 ?iCna> CONFIDENCE IMlTS Afeff 95`. LiniTS AS X OF MtAN ARE 73 4rH9 Ttv.: 10 mICn UET ?4 3932 52 (? SICnA) CONFIDENCE UITS ARF 14 231? TO 41 809S 9 5/. LIMITS AS 2 OF MEAN ARE 58 3437 2 TO 171.398 2 Ta'-lS 10 CONTROL LIPID H-4N 412?67 S2 ! 2 SICma > CONFIDENCE 1ImJTS ARF 1270S2 TO 1 3339 ?S2 LIMITS AS X OF MEAN ARE 30.9S9S X TO 323 003 X TAKE 10 LOU LIPID HE AN s 677S86 9SX <2 SICMA) CONFIDENCE LIMITS ARF 952 LIMITS AS X OF MEAN ARE 38 734? 262462 TO 1.7492? : TO 2S9 16S X TAKE 10 MEDIUM LIPID ME an 0 85754 X <2 SICMA) CONFIDENCE LIMITS ARF 6.3633? T 952 LIMITS AS 2 OF MEAN ARE 73.0673 X TO 138 TAKE to HIGH LIPID ----------- MEAN 3S.A48S 9SX <2 SICMA) CONFIDENCE LIMITS ARF 16 7222 TO^ 75 952 LIMITS AS X OF MEAN ARE 46 9085 2 TO 213 iei 2 mTAaKnE 11 1CnO2oN6T8ROL UET 9SZ (2 SICMA) CONFIDENCE LIMITS ARF 102031 TO 2S934? 952 LIMITS AS X OF MSAN ARE 62 7234 X TO 1S9.43 X TAKE 11 LOU UFT MEAN 9SX 12 2.S0306 SICMA) CONFIDENCE UNITS ARE 1.62661 TC! 3 430C7 9S2 LIMITS AS X OF MEAN ARE 72 9746 X TO 137 034 X 54 HONS 008525 taf-lE ii COIUH WET nAN B *61*6 9X <2 SIGMA) CONFIDENCE tsx LIMITS AS X OF MEAN A TAFLE 11 MICH UT ME an 20 S02B 957. <2 SIGMA) CONFIDENCE LIMITS ARF! 10 *36* TO *0.27*6 9SX LIMITS AS X OF MEAN ARE 50.9023 X TO 19$ *55 X TAFLE 11 CONTROL LIE ID MEAN 213713 957. (? SIGMA) CONFIDENCE LIMITS ARE 9S;.' LIMITS AS X OF MEAN ARE 57.13** 1t253103175T.0O26 37*053 X TaFlS 1l LOU LIPID mean 2 77641 9*7. <2 SIGMA) CONFIDENCE LIMITS AF 1 80326 TO * 2A089 9SX LIMITS AS X OF MEAN ARE 6*.9027 X TO IS* 077 X TAt-LE 11 MF0IIJM LIPID n-.an ) 1 56** (2 srCMA? CONFIDENCE LIMITS 7 85**2 TO 17 0)62 9 57. LIMITS as X OF MEAN ARE 67.9533 X TO 1*7.16 X 11 HIGH LIPID 30 038* 2 SIGMA) CONFIDENCE LIMITS ARE U BS3* TO 76 09 IMITS AS 7. OF MEAN ARE 39 *77S 7. TO 253 309 X SP-S9T-S8S *2 STCMA) CONFIDENCE LIMITS aRf *7622 TO 1 30222 5>: LIMITS AS X OF MEAN ARE 5? 9*82 X TO 178.737 X TAFLE 12 LOW WFT MFan ? 86636 957. (2 SIGMA) CONFIDENCE l 1MJT5 ARF 2. 1**02 TO 3 86577 9SX LIMITS AS X OF mean ARt 74.;80S X TO 1?* 624 X TAFLE 12 LOU UF.T MF an 2 88638 9*2 <2 SIGMA) CONFIDENCE LIMITS ARF 2 1**02 TO 3 66577 9SX LIMITS AS Z OF MEAN ARE 74.2808 X TO 13* 62* X TAFLE !2 MF0IUM UFT MFan 12 *316 SX (? SIGMA) CONFIDENCE I IMITS ARF 95Z LIMITS AS v Ar *" TAKE 12 MJGN UFT man 19 936* I95* 9S>. <2 SIGMA) LIMITS AS CONFIDENCE I X OF MEAN AS MITS 2 APF 1757 29 X 82*5 TO TO i385 51 55 7. 112* TAFLE 12 CONTROL LIPI0 MEAN 1 038* 95X <2 SIGMA) CONFIDENCE LIMITS ARE .530677 TO 2.03229 9S2 LIMITS AS x OF MEAN ARE St 1002 X TO 195 6* X 55 008526 o?sr>:.. L<2IMrITiSc*Sp c2 oOnFfidMeEnAcNe Alliitmit6s6 TAWlE 12 HFDIUM LIPID mean 17 3316 9ST' < 2 SICN4) CONFIDENCE LIMITS A#F 11 319* TO 26 S38 9SZ LIMITS AS X OF MEAN APE AS 3096 X TO 153 117 X S-8KSS-2 P*9 9 TA&lE 12 MICH l IP ID MF AN 97 9947 SZ (3 MGrtA) CONFIDENCE LIMITS ARF SB.0866 TO 165 322 95\ LIMIT*. X OF MEAN ARE S9 27S2 * TO 168 70S 2 T&fc_C '3 cnh.T&0' u-T r- Am * 37J&32 9 ;> < *V: L XA70911 tav.E 13 LOiJ wfT <=?: <2 SIC*'*) CnN^lDE^cS l IrlTS A3' 2 707aa TO S SIS*? 5*: LIMITS AS T G* H3 Am A* 70 0*32 :: TO 142.728 X ` ) 3 M- Dl'jn LIFT i * `6 3S21 ? S7THA' CONFI PENCE ' INITS af LIMITS as X t/F nAN fiPE 76 ! 776 TQ 20 92*A TC1 127 99? X f M.* '3 HjCM UrT nMi>**. '1 (2 3SbIC9M2t5i 5 CONFIDENCE LIMITS AM \9 S^Ol TO 77 r?A* 9SI; LIMITS AS X OF mean *ftc 50 S3.-7 X TO tv? 8*2 X TAliui 13 CONTROL LIP ID *:in 632762 <? S'OMA. CONFIDENCE LIMITS ARF 95* LIMITS AS X OF hEAN AP S3.2947 1 3 LOU -UeT A: ; 9SX 95* 5 6A38 <2 SIGMA) LIMITS AS CONFIDENCE LIMITS Z OF M?AN ARE 56 ARF 8682 3 X 20953 TO 9 ??OS To 175 MS * TMLE 13 MEDIUM LIPID MFAN 23 5652 9SX (2 SIGMA) CONFIDENCE UNITS ARE 16 611) TO 33 4*06 95X LIMITS AS X OF MEAN ARE 70 4099 X TO 141 864 X TAfruE <3 HIGH LIPID 9S?W<2 SIGMA) CONFIDENCE LIMITS ARF 62 6862 TO 908.357 9SZ LIMITS AS z OF MEAN ARE 54 8507 X TO 182 313 Z TAk>LC 3 4 CONTROL UET mean 292317 SX (2 SIGMA) CONFIDENCE 1 INITS ARE 9SX LIMITS AS X OF MEAN ARC 47.70 TAKE 14 LOU UET MEAN - 3.35533 9SZ 9SZ <? SIGMA) LIMITS AS CONFIDENCE LIMITS ARF Z OF MEAN ARE 65.3748 2 X 1 5 1324? Z 56 HONS 008527 TaKE M MFDIUH UET WfAN 20 2384 SV. <2 SIGMA) CONFIDENCE LIMITS ARF 10 7382 TO 38 1434 9SX LIMITS AS X OF MEAN ARE S3.QS87 X TO 188.471 X TAKE 1 * HTCh UET hr&n * 95 82*5 ?S7. <? MCHA> CONFIDENCE lIMITS ARE 70.9561 TO 129.414 9SX LIMITS AS X OF MEAN ARE 74 04*5 X TO 135 OS X TAKE 1* CONTROL LIPID MEAN - *44225 957. (2 SIGMA) CONFIDENCE LIMITS A*? 957. LIMITS AS X OF MEAN ARE 23 4*90 lSll9e TO 2 74491 ; TO 43&.079 X TAKE ) 4 LOU HMD MEan *.32*52 95V. (2 SIGMA) CONFIDENCE LIMITS ARE 3.53561 TO 1 i .3712 ASX LIMITS AS X OF MEAN ARE S58S*1 X TO 179 022 X TAKE 14 MEDIUM LIPID M'AM - 28 A) 38 05*. <? SIC-*) CONFIDENCE I ImITS *3r 12 e9l7 TO 64 8S7 9?V LIMITS AS 4 OF ME.4* ARE 44 5532 X TO 224 456 X TAKE 1 4 HIGH LIPID man * i6? S3 5t. (3 SIGMA) CONFIDENCE I.I 954 LIMITS AS Z OF man ARE ,3S A6R91 103 773 TO 264 04 7. TO 159.512 X TAKE 15 CONTROL UET HFftM 120589 95X (2 SIGMA) CONFIDENCE LIMITS a 954 LIMITS AS X 0? MEAN ARE 45.6799 283*8* X TAKE IS LOU UCT mean > 2.20332 5X (J SIGMA) COMFIDgNCE IIM 9s4 LIMITS AS X OF MEAN ARC . 553*3 TO 14 TAKE 15 MEDIUM UET 95X**< 2 SICMA)"CONFlDENCE LIMITS ARE 10 Si4 TO 23 893 95X LIMITS AS X OF MEAN ARE 66 3305 X TO ISO 742 X TAKE 15 MICM UPT MEAN 42 3203 95X (2 SIGMA) CONFIDENCE LIMITS ARE 9SX LIMITS AS x OF MEAN ARE 67.431 62 7609 X 5-8MS-2 P9 10 HFan 22506 657. * 7 s!5mA) COnf ID jWCE i IMITS AF .07'73|5 TO 69418* 9SX LlMifb AS X OF MtAn APt 32 327* X TO 309 333 4 T**Ar V-NLE * <15 L9O3U295LIPID 94*/. < 2 SICMA > CONFIDENCE I IMITS 95;: LIMITS AS X OF nEAf* ARC S4 2SS. 2 213C5 TO 6 99304 TO 177 761 X 57 MOMS 000528 Tau. *S M9ED*nIUfM LIP ID *.>. (? SICn* CONFIDENCE UMITS ARP 20 871P TO 4S 6?4 LlnlTS AS 2 OF MEAN ARE 67 b\+t> 7. TQ 1 47 897 X TAKE IS HI CM LIPID . ("an - Mb 7S * S7. (2 SIGMA) CONFIDENCE l IHITS ARE 71 1216 TO 189 767 95X LIMITS AS X OF MEAN ARE 61 2196 X TO 165 347 X TAKE 16 CONTROL WET HE AN 120773 9S:: C2 SIGMA) CONFIDENCE LIMITS ARE 9S:; LIMITS AS X OF nEAN ARE 6S 6ldl 183494 X TAKE 16 LOW.WtT Hf AN 13 0617 *V/ (2 S1GMA\ CONFIDENCE LIMITS ARE 9 65429 TO 17 6717 9 S': LIMITS AS X OF MEAN ARE 73 913 X TO 135 294 y. TAKE 16 WICW WET MEAN ic 0199 9SZ (2 SIGMA) CONFIDENCE LIMITS ARf 4 30186 TO 23 3385 9SV. LIMITS A5 X OF MEAN ARE 42 933 X TO 332 9*1 X TAKE !i CONTROL LIPID MrAN 3349 (2 SIGMA) CONFIDENCE LIMITS AR 223398 TO .S020S3 9 5*'. LIMITS AS X OF MEAN ARE 66 7061 X TO 1*9 ll X Th;A*KnE 1624 HFDIUM 9736 LIFID SX (2 S---I-G---M---A--? CONFIDENCE lUMITS Apr 17 394? TQ 35 957. LIMITS AS X OF M*AN ARE 69 6Sl3 X TO 143.57 X TAfc'.E 16 HTCN LIP ID H"AN 34 S144 9SX <2 SIGMA) CONFIDENCE UMITS ARF 95X LIMITS AS X OF MEA" 6X8 8134 Take 1 8 CONTROL MET rFAN 434785 9X <2 S1CMA) CONFIDENCE UMITS ARF 243576 TO 776094 957- LIMITS AS X OF MEAN ARE 56 0222 X TO 173 501 X TAKE ) LOU UET HFan 3 02133 SX <2 SIGMA) CONFIDENCE LIMITS ARE 2 55449 TO 3 S7349 9SX LIMITS AS X OF MEAN ARE 84.5404 X TO 118.275 X TAKE 10 MEDIUM UET NfAN 15 5034 9x 12 SIGMA) CONFIDENCE LIMITS arf 9SX LIMITS AS X OF MEAN ARE-- 66 0994 TAKE 18 HIGH UET MFAN 43.2059 95X <2 SIGMA) CONFIDENCE LIMITS 9SX LIMITS AS X OF MEAN ARE SO APF 7413 21 X TQ23:2197 TOQ78 85Z 1468 MTAAKwE -181.4C0O0N2T4ROL LIPID 9S* (2 SIGMA) CONFIDENCE t IMIT5 ARF 669F TH 3 27131 9S7. LIMITS AS X OF MEAN ARE 45.2*93 : TO 22 0 99p 7. S-3MS-2 P;; )1 58 HONS 008529 Tah-.E >0 LOU LIPID 5 06448 SV, <? SlGhA) CONFIDENCE ITM1TS A4 9S*: Linns AS X Of HEAN ARE S3 4*64 T *S _S 18 a*-DION LIPID mFan a 38 584 !-. <2 S1C*A' CONCIO^nCI IMITS APF IS SO27 TO 90 2344 9S>. Linns aS X OF nEAN are 42.76S3 X TO 233 835 2 S'tMS.Z P*5I u ol>:: (2 SIGmai CONFIDENCE tiMITS ae 4S $477 TO 206 *4 4S:; LIMITS AS 2 OF A 46.9144 X TO 213.152 X SIC**) CONeiDrNe LIMITS APE 332376, TO S72S88 mis AS X Or HcAN A?* 7b 189,? X TO 13i 252 2 T r.v- r i i rg w-T n--** 2 3-0 1 Sr "=*- (2 S IGnA > CONFIDENCE JtMITS B? 1.63315 TO 2 90364 S*: LIMITS AS CF hAr* AP 79 2174 Z TQ 12b 235 X i i.s nFDIUn u?T . 1* (M7 >2 =IGnA) LinITs AS C0N?iriNC X 0 MEAN Tn:T APE b ? 9 40519 TO 20 827 Z TO t49.044 X A' HTCH UCT . 4 w<2 S . j nl' limits ap? . 74.420: Tt*.' F 1 C0NT&nL LIPIO r?i>. * o-rp-7^ cr-. ,2 CONFIDENCE LIMITS AF 332693 TO 2 604\3 9j`. Linn? Ai x O' n*N Jr> 7*^1 X TO 379.78 X t*Vl 19 LOU LIPID -* ? 949S8 4sx 95X <2 IGma> LIMITS AS Confidence x CF nlAN ^nTT7?f a94--V3 TAKE 19 HFDUm LIPID MS AN 17 2633 > 5X (2 SIGNAL CONFIDENCE i IMITS AF tl 6183 TO 25 651 9 5*; LIMIT* AS \ Of MEAN APE 67.3007 X TO 140 587 X T - *> _ ' I II5 w**" s*C>a>9C Oh? ]&?*(.* 'InITS APS 31 CS2? TM 61 2473 *52 LlnlTS AS X v* nfcAN A-i 7i 2-..-' X TO 140 373 X 59 HONS 008530 Ct *6,ii i i ionr i. HONS 0 0 0 5 3 1 riGUtt ik PCI In Adult fit Saplt - lipid Wright Bails i i ' t j jM S m p>"n - Naa rpn Wet Wt. Oatts Control low Ned turn High Table 6 Initial A*erajei_(log TAflf II Trf4tmrntf 4nH Control Table 7 1 No. Table 9 3 No. Table 10 4 Ho. 1l.ihlr n Mo. Table 1? table I) Table 14 9 Hn. _ _ H.No __ Table 15 14 Ho. Table 16 16 Ho Table I* Table 19 11 Ho. 20 Ho. 0.749 0.495 0 .585 0.688 0.236 0.42? 1.733 10.916 o.sai 0.964 5.993 70.100 0.748 0.441 7.459 74.393 0.161 2.50) 8.4191 70.501 0.771 2.RI16 12.43? J9.9J7 0.177 3.064 16.352 30.976 0.217 1.355 20.730 95.*?/ 0 UO 7 201 15.050 42.3?0 0.171 -- 13.061 10.020 0.43$ 3.071 15.503 43.210 0.436 2.300 14.01* 32.420 y pid w. * y} Control!i low Medium High 1.716 0.753 0.915 0.953 0.3BB 0.534 2.140 14. mi 1.240 1.965 7.609 32.034 0.413 0.67B 8.*5* 35.649 0.714 7.778 11.564 30.038 1.039 4.700 17.33? 97.995 0.63) 5.(44 23.565 114.285 0.644 (.330 78.914 165.530 0.755 3.934 30.869 116.175 0.335 -74.974 34.514 1.480 5.065 38.589 97.086 0.931 2.950 17.211 43.61? HONS 0 0 8 5 3 3 S-81-SS-2 Page 16 TABLE III Individual Statistical Comparisons Adult Fat Samples PCB Found - Vfet Height Basis 1. Pre Drug 2. 1 Month 3. 3 Month 4. 4 Month 5. 7 Months 6. 9 Months to 20 Months No significant differences found. Control significantly lower than pre drug control. High level significantly higher than all others. Control significantly higher than 1 month control. High level significantly higher than all others. High level significantly higher than all others. Medium level significantly higher than low and control. Medium level significantly higher than 1 and 3 months medium. All levels differ signi ficantly from one another. Low level significantly higher than 4 months low. Levels remain significantly different except for sharp drop in 16 Month high level data. No further increase in amount found. PCB Found - Lipid Weight Basis 1. Pre Drug 2. 1 Month 3. 3 Month 4. 4 Month 5. 7 Months 6. 9 Months 7. 9 Months to 20 Months - No significant differences found. - High level significantly higher than low and control. - High, medium and low significantly different from one another. High and medium significantly higher than control - Same as 3 Month. - High and medium significantly higher than low and control Low significantly higher than control. - All levels significantly different from one another. - High and medium differ significantly from low and control - High significantly higher than medium except 18 and 20 months. - Low significantly higher than control except 20 months. 63 HONS 008534 64 HOMS 008535 !I 65 MQNS 008536 S-81-SS-2 Page 19 ^arjle Taken 5/24-6/9/78 5/19-6/30/78 7/6-7/31/76 8/8-10/10/78 10/27-12/11/78 TABLE IV EBby Skin - itt Meloht ftsis - Averaoes (PPM) Control Level Fed Low Medium Hiqh .083 .396 .826 .650 -- .175 2.26 2.42 6.84 1.53 1.45 2.92 16.60 13.84 12.59 1.82 13.80 -- Saiwle Taken 6/19-6/30/78 7/6-7/31/78 8/8-10/10/78 10/27-12/11/78 Baby Fat - Met Ueioht Basis - Averaqes (PPM) Level Fed Control Low Mediurn Hiqh 1.04 0.70 0.61 -- 2.03 10.69 9.04 0.76 8.32 98.17 18.11 108.89 38.90 -- 66 HONS 008537 TAELE V PPM PCB Found in-Hi 1 k Samples Level Fed Control (0.00) Low (5 PPM) Medium (25 PPM) High (100 PPM) PC E Found .08 .01 .03 .20 .02 .03 0.22 0.01 0.01 0.42 0.51 0.46 0.45 0.95 S-81-SS-2 Page 20 67 l HONS 008538 S-81 -SS-2 Page 21 PRcPARcD 3Y : Oij^w* J. b. Hinchen __ Senior Research Specialist Date: / Applted Sciences Computer Sciences/ Statistics Group /bjs k HONS 008539