Document pmdYMaq2K1nq80DNOOw15B2vj
Unit Code 8308 1/14/89 2:54PM UROBILINOGEN, 48-HOUR, FECES
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Seneral Information
FEE: $122.70 LABORATORY AREA: Porphyrin32/HC25 ADNAYA(LSY)TTIECSTTIMSEE:T 1UPd:ayMonday through Saturday TIME OF DAY: Continuously LABORATORY HOURS: MAXIMUM LAB TIME: 3 days COMMENTS AND OTHER REJECTION: CV 6%. If thawed but cold, note to laboratory. If RANDOM SAMPLE is rec'd, REJECT.If client nsistsontesting: 1. Consuitation with Dr. McConnell must occur beforelabwill accept
specimen and perform the assay.
2. We have NO NORMALS for a RANDOM specimen. 3.Iftestis run, the report will be as gramsof specimen. Should be a 24, 48 or 72 hour collection. Lab will NOT do on anything ther than the above listed timed collections. Please do not call the routine laboratory for approval of specimen not meeting collection duration criteria. SPECIMEN RETENTION TIME: Until reported LAB CONSULTANT TO CONTACT 1st: Dr. McConnell 47437 LAB CONSULTANT TO CONTACT 2nd: LAB CONSULTANT TO CONTACT 3rd: CLINICAL CONSULTANT: CLINICAL SERVICE: TECH TO CONTACT fst: Paul Chezick 4-8081 TECH TO CONTACT 2nd: Chuck Kroll 4-3232 LABORATORY: Porphyrin 4-3232
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"Unit Code 8308 1/14/99 2:56PM
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UROBILINOGEN, 48-HOUR, FECES
"dethod and Reference
METHODOLOGY: Colorimetric
METHOD: Urobilin in the feces is reduced ta urobilinogen by treatment with alkaline ferrous hydroxide. The urobilinogen is then allowed to react with Ehriich's reagenttoforma red color which can be measured `spectrophotometrically.
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Unit Code 8308 1/14/99 2:56PM
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UROBILINOGEN, 48-HOUR, FECES
tinical Information
CLINICAL INFORMATION: - Urobilinogen consistsofthe colorless end productsofbilirubin metabolism which are oxidized by intestinal microorganisms to brown pigments (stercabilin). - Normally, fecal urobilinogen approximates the total excretion of bile pigments (the breakdown productsof heme).
USEFUL FOR - Diagnosisofdisorders involving hemolysis and hepatic obstructive conditions
INTERPRETATION - Increased destructionofred blood cells, such as in hemolytic anemia, increases the amount of urobilinogen excreted. - Liver diseases in general lessen the flow of bilirubin to the intestine and thus decrease fecal excretionof uroblinogen. - Complete obstruction of the bile duct reduces the urobliinogen of the feces to verylow levels. Clay-colored feces in obstructive Jaundice resus from the exclusion of bile pigments from the intestine. -Oral administration of broad-spectrum antlbiotics diminishes conversion of the bilirubin to urobilinogen in the Intestines.
CAUTIONS - Information about oral broad spectrum antibiotics taken recently by the patient must be accurate. Oral broad spectrum antibiotics decrease urabiinogen measurements by diminishing bacterial conversion of bilirubin to urobilinogen in the feces, leading to faisely low values. IMMEDIATE fixationofspecimen by the prescribed preservative (sodium carbonate/petroleum ether) in an opaque container is essential, since urobilinogen is light sensitive. Poor fixation gives a falsely low reading.
REFERENCE ~ Schwartz S,
Sborov
V,
Watson
CJ:
Studies
of
urobilinogen:
quantitative determination of urobilinogen by meansofthe Evelyn
photoelectric colorimeter. Am J Ciin Pathl 14:508-804, 19944
.
.
.
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