Document pmZ7vGmE2JrKy1bLqeJRY75jB

ACTIVATED SLUDGE RESPIRATION INHIBITION TEST EEX TESTSUBSTANCE -- _-- - Identity: PFCe-r8f5l.uor(o1o-cOtacnteasnuelsfuolnfaotnei;c macaidy,a1l,s1o,2b.e2.r3e,f3e.r4r,e4d.5t,o5,a6s.6P.7F.O7.S8.o8r.8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: from 3M lot nuTmhbeetres2t17s.ubsStaamnpcleeiswaaswhsittoerepdowudnedre.r Sample ambient was taken condifions, pNrMioRr taontdesteilnegm.enPturailtyandaeltyesremsinteedchtnoiqbuees9.0.49% by LC/MS, 'H-HMR, '*F- METHOD a EE Method: OECD 203 Test type: Stalic acute GYeLaPr: CYoemspleted: Study completed 1999. Report completed 2000 Analytical Statistical monitoring: Dissolved oxygen concentrations. methods: Probit analysis using the computer software of C.E. Stephan Test organism source: Activated sludge collected from the Prospect Bay Wastewater Treatment Facility, Grasonville, Maryland. Test conditions. Dilution water: NANOpure Synthet1ilcitSeremwuangieci:pal water 16.0 g peptone 11.0 g meat extract 30g urea 0.7 g NaCl 0.4 CaCl, 2H.0 0.2 g MgSO: 7TH,0 28g KHPO, Rreeffeerreenncecesuabnsdtatnecset,s3o,l5u-tdiiocnhiporreoppahreantoil,onw:aAs stock solution prepared by of the dNiAsNsoOlpviunrge500wamtegr,inth1e0nmbLrooufghItNtNoatOheH,podiinltutoefdintcoi3pi0enmtL with precipitation with water. The pH of 1N the H;SO,, and diluted to 1 reference solution was L with NANOpure measured (0 be 7.18. 0086 PFOS was added directly to test vessels rather than volumetric addition of a stock solution. This method was deemed appropriate based on the observed solubility of the test substance in water. Test vessels: Mixtures were prepared and aerated in 500 mL Erlenmeyer flasks and then transferred into 300 mL Biochemical Oxygen Demand (BOD) bollles Number of concentrations: 7 plus 3 reference controls and 2 Blank controls Temperature: 19-21C Total Suspended Solids and pH for sludge on dayof testing: 4380 mg/L and 7.87 respectively. Element Basis: Respiration inhibition as determined by dissolved oxygen concentration. Method Remarks: Stock solutions of PFOS that were prepared at nominal concentrationof approximately 500 and 1000 mg/L in NANOpure water contained test material that was not in solution after 20-minutes of sonication. Therefore, direct weight addition was employed to administer PFOS to the test system. Test mixtures were prepared at 15-minute intervals and aerated unt the contact time of the test substance with the activated sludge was three hours. After 3-hours of contact time, dissolved oxygen was measured over a period of up to 10-minutes. RESULTS oo oo } Nominal concentrations: Two blank controls, three reference substance controls, 0.90, 2.7, 9.0, 27, 90, 271, 905 mglL test material solutions Statistical Analyses: ECso values were calculated for the reference material by probit analysis using the computer software of C.E. Stephan. An ECs value could not be calculated for the test substance. Analytical Methodology: Analysis of DO concentrations in all test solutions were performed at Wildiife International Ltd. using a YSI Model 508 Dissolved Oxygen Meter. Dissolved oxygen readings were recorded every 10 seconds for 10 minutes or until the dissolved oxygen dropped below 1.0 mg/L 00865 Respiration Rates and Percent Inhibitions CTT reawenLot [GoTomori [[53.d55diethlolroopnrenoopl ShTsemmnaooll T3.e5s-tdsicuhblsotraonpcheeno0l 9500mmlg Test substance 27mglL TTeessttssuubbssttaannccee 92.70mmogllL TTeessttssuubbssttaannccee 02071mgmai_ Test substance 605mal |Re"mg sCpiuahtioonuRrate || Pericnhiebitniont || C"aSes-- NNATA eTa e mGme 8591 -- 35 - m1 Bo - "3a5ro 327 r6o 1 | wo : 2a0r1 m30s4 Control response: satisfactory CONCLUSIONS rTehsepitreasttiosnuabtstaannocmeienxahlibtietsetdsaubmsataxnicmeucmonicnheinbtirtaotriyoenffoefct90of5 3mg8/%L.upTohne tEeCsts(urbesstpainrcaet.ion inhibition) is therefore greater than the solubility of the SStu.bmPiautlt,erM:inne3sMotCao,m5p5a1n3y3, Environmental Laboratory, P.O. Box 33331, DATAQUALTY Reliability: Kiimisch ranking 1 REFERENCES "rTehqiusessttudofy twhaes3cMonCdoumcptaendy.at Wildlife Intemational, Ltd. Easton, MD at the OTWER Last changed: 5/3/00 00SG6