Document pmYJMRGmQR44X796d5Kg1aXwX

M S.A6 - 0 7 6 0 ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE________________________________________________ Identity: Mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203CF-X or FC-203CF. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 101. The test sample is FC203CF. Current information indicates it is a mixture of 1% PFOS, 20% diethylene glycol monobutyl ether, 70.2% water, 2.75% Acrylic foamer, 3% sodium octyl sulfate, 1% triethanolamine, 2% sulfonated alkyl ether salts, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component o f the test sample. METHOD:_________________________________________________________ Method: OECD Guideline 203. Type: Acute static GLP: No Year completed: 1991 Species: Pimephales promelas Supplier: Aquatic Research Organisms, Division of Resource Analysts, Inc., Hampton, NH. Analytical monitoring: Temperature, pH, conductivity, and dissolved oxygen. Exposure period: 96-hours Statistical methods: No calculations performed, based on study results. Test fish age: Juveniles Length and weight: Average length = 34 mm Average weight = 0.23 g (wet) Loading: 0.15 g/L Pretreatment: None Test conditions: Dilution water: Filtered natural well water Dilution water chemistry: Hardness: 80 mg/L as C a C 0 3 Conductivity: 830 pmhos/cm pH: 7.7 Lighting: Cool-white fluorescent lights. A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. Stock and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis. 001217 Concentrations dosing rate: Once. Stability of the test chemical solutions: Not noted. Exposure vessels: 20 L glass aquaria (40x20x25 cm) containing 15 L of test solution and an approximate water depth of 18 cm. Number of replicates: 2 Number of fish per replicate: 10. Number of concentrations: five plus a blank control Water chemistry during the study: pH range (0-96 hours): 7.1 - 7.7 (control exposure) 7.5 - 7.7 (1000 mg/L exposure) Temperature range (0-96 hours): 2 1 .8 -2 2 .7 C Conductivity range (0-96 hours): 830 - 850 pmhos/cm (control exposure) 850 - 910 pmhos/cm (1000 mg/L exposure) Dissolved oxygen range (0-96 hours): 6.8 - 8.1 mg/L (control exposure) 2.5 - 8.5 mg/L (1000 mg/L exposure) RESULTS_________________________________________________________ Nominal concentrations: Blank control, 150, 250, 400, 600, and 1000 mg/L Element values: 96-hour LC50 = >1000 mg/L Element values based on nominal concentrations Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS___________________________________________________ The test sample 96-hour LC50 for fathead minnow was determined to be >1000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY____________________________________________________ Reliability: Klimisch ranking = 2. Testing meets the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. 001218 REFERENCES_____________________________________________ This test was conducted by EnviroSystems Division, Resource Analysts, Incorporated, of Hampton, NH at the request of the 3M Company, St. Paul, MN, Lab Request number J 1884-3,1991. O T H E R ___________________________________________________________ Last changed: 6/28/00 001219 A V A IL A B L E " 6. f# The acute toxicity of FC-203CF to the fathead ainnow, Pimephalea proaelaa, is described in this final report. The test was conducted for 3M Conpany for 96 hours during June 3 to 7, 1991, at the KnviroSysteas Division of Resource Analysts, Inc. in Haapton, Hew Haapshire. It was conducted by Jeanne Hagazu, Peter Kowalski, Ellen Stanford, Robert Boeri, and Tiaothy Hard . The test was perforaed under static conditions with five concentrations of test substance and a dilution water control at a aean teaperature of 22 1C. The dilution water was filtered natural well water collected at Raapton, Hew Haapshire. Aeration was not enployed to aaintain dissolved oxygen concentrations above an acceptable level. Hoainal concentrations of FC-203CF were: 0 ag/L (control), 150 ag/L, 250 ag/L, 400 ag/L, 600 ag/L, and 1,000 ag/L. Hoainal concentrations were used for all calculations. Fish used in the test were purchased froa a coaaercial supplier .(Aquatic Research Organises, Haapton, Hew Haapshire) and accliaated under test conditions for nore than 15 days. After 96 hours of exposure the control fish had an average total length of 34 aa and an average wet weight (blotted dry) of 0.23 g, resulting in a loading rate of approxisately 0.15 g/L. All fish were in good condition at the beginning of the study. . Exposure of fathead ai.nnows to the test substance resulted in a 96 Vi-hour LC50 greater than ';.1,000^ ag/L FC-203CF. Two of the twenty fish ^.exposed to 600 ag/L-.at^2~hours: and all of the fish exposed to 1,000 , %ag/L froa 24 to 96 .hours hiwere lethargic and exhibited a loss of equilibrium. Ho other j sublethal effects were observed in any test .v vvessel during the 96 hour exposure^;. :f> >h4<v: : wr-v.jeV'r V-'- - ^ `Vs yx1-:/ ' 'w ' *' `yl'l/s*! v-i> tf.E lo f-1 4 *%(*?> ~5i SubMoyolMIUPOnE : pr%VA- 001221 BEST COPy AVAILABLE III. MDBC Of TABLES Table 1. Cheaical characterization of a representative saaple of natural well water used as dilution water for toxicity test Table 2. Survival data froa toxicity test Table 3. Hedian lethal concentrations (LC50s) froa toxicity test Table A.l. Conductivity, pH, teaperature, and dissolved oxygen concentration neasured during toxicity test PAGE 6 9 10 13 BEST COPY AVAILABLE i v . ihtrowjctiom This study was sponsored by 3H Cospany, St. Paul, Minnesota. The objective of the study was to deteraine the acute toxicity of FC-203CF to the fathead ainnow, a freshwater fish. The report contains sections that describe the eethods and aaterials enployed in the study, and the results of the investigation. The report also contains an appendix that presents the water quality data collected during the test. V. METHODS AMD MATHI ALS TEST SUBSTAHCE: FC-203CF (EnviroSysteas Saaple Ruaber 2937E) was delivered to EnviroSysteas on May 23, 1991. It was contained in a 4 or plastic bottle that was labelled with the following inforaation: "J1884-3, FC206CF". FC-203CF (an orange-brown liquid} was supplied by 3M Coapany, 935 Bush Avenue, St. Paul, Minnesota. Prior to use the test naterial was stored in the dark at rooa teaperature. A reserve saaple (approxiaately 1.0 graas) will be archived at EnviroSysteas for a ainiaua of 10 years. DIL0TI0M WATER: Water used for accliaation of test organises and for all toxicity testing was collected froa wells at EnviroSysteas in Haapton, Mew Hampshire. Water was stored in 500-gallon polyethylene tanks, where it was aerated. Results of cheaical analysis of a representative saaple of water are presented in Table 1. TEST ORGAMISM: I;?>!:^V:`-.WVwJJ.-.V-vv' .",<v-V-- .-' t'.-;"t - , ' V , ' *' v->. /c:i ' '. , ' ' :' . . V '-J. .. * . .: f -.- BEST C O P t AVAILABLE tK.>S%'. ' Tahit i. Chemical character'ration of a representative sample of natural tall water used aa dilution.water for toxicity teat Parameter - 'V' Unit of Measurement Reporting Limit Measured Value Hardness mg/L as CaCOs 80 Organochlorine ug/L 0.5 HO pesticides 'J>i* 1 Organophosphorus pesticides V . - V `-r^Fv' " ug/L .. 1*V. - 'j HD Polychlorinated biphenyls- vm ^ ug/L 0.5 -, /ili HD j MD. npt' detected 'at.'b r'ra b o v e 0 h ^ ^ r , t i u g ^ ; l i i i i t V *i^,, tphi'ihadess^and .coirutivl^^ iv ater .collected fro k lth eic o n tro Iitte st.v esselD rio r tai2trC ; ; ! '- '& & & .V '.v',.*'.. -V> . I ^' V - v y; - , 1?' / IJ&Av* '* BEST COPY AVAILABLE TOXTCITT TESTING: r A screening test with the test substance was conducted during Hay 24 to 28, 1991. The test was conducted at 1, 10, 100, and 1,000 ag/li and after 96 hours there was 100% survival at all concentrations. The definitive toxicity test was performed during June 3 to 7, 1991. It is based on procedures of the OKCD (1984). The test was conducted at a target temperature of 22 t 1C with five concentrations of test substance and a dilution water control. Ho stock solution was prepared as test aaterial was added directly to dilution water contained in the test vessels without the usp of a solvent. Noeinal concentrations of the test eaterial were: 0 mg/L (control), 150 mg/L, 250 eg/L, 400 eg/L, 600 eg/L, and 1,000 eg/L. Twenty fish were randomly and equally distributed among two replicates of each treatment. The test was performed in 20 liter glass aquaria (approximately 20 cm in width, 40 cm in length, and 25 cm in height) that contained 15 liters of test solution (water depth was approximately 18 cm). Test vessels were randomly arranged in an incubator during the 96 hour test (a random numbers table was used to select the location of each vessel). A 16 hour light and 8 hour dark photoperiod was automatically maintained with cool-white fluorescent lights. Aeration was not employed to maintain dissolved oxygen concentrations above acceptable levels, fish were not fed during the test. The number of surviving organisms and the occurrence of sublethal effects (loss of equilibrium, erratic swimming, loss of reflex, ' excitability, discoloration, or-'change in behavior) were determined visually and recorded initially'and after 24, 48, 72, and 96 hours. Dissolved oxygen (YSI Model 57 meter;' instrument number PRL-3), pH r(Beckman model pHI 12 meter;, instrument number PRL-51), conductivity , (Cole;;.Parmer model .number .1481-60,'. instrument number PRL-50), and temperature ;,(ASTH mercury thermometer).thermometer number 1686) were measured .and recorded 'daily in each test 'chamber that contained live lAnotynl 00 YI. XKSULTS All teat vessels containing FC-203Cr were initially clear and reaained clear throughout the test. Biological and water quality data generated by t he. acute toxicity test are presented in Table 2 and Appendix A, respectively. One hundred percent survival occurred in the control exposure. Control fish had an average total length of 34 an, and an average wet weight (blotted dry) of 0.23 g at the end of the test. Loading rate during the toxicity test was approximately 0.15 g/L. .. s." > The 24. 48, 72, and 96 hour LC50s for fish exposed to FC-203CF are presented in Table 3. The 96 hour LC50 value is greater than 1,000 ag/L FC-203CT. Two of the twenty fish exposed to 600 ag/L at 72 hours and all of the fish exposed to 1,000 ag/L fron 24 to 96 hours were lethargic and exhibited a loss of equilibriua. No other sublethal effects were observed .in any test vessel during the 96 hour exposure. : m . *- " *v. Tbl* 2. Survival data fro toxicity test Noainal Concentration (ag/b) rep. Wueber Alive Ohr 24hr 48hr 72hr 96hr Nuaber Affected Obr 24hr 48hr 72hr 96hr 0 (control) 1 ,- ,\ 2 10 10 10 10 10 10 10 io 10 10 0000 0 0000 0 150 1 10 10 10 10 10 1 0 0 0 0 0 2 10 10 10 10 10 00000 250 1 10 10 10 10 10 2 10 10 10 10 10 00000 00000 400 r x / 1 10 10 10 10 10 " 0 0 0 0 0 - ." 'V'"J 2 10 10 10 10 10 00000 600 ' ', 1 10 10 10 10 10 2 10 10 10 10 10 0 0 01 0 00010 1 ,0 0 0 . tc?:-' 1 10 10 9 9 9 . 0 10 9 9 9 2 10 10 9 9 9 - 0 10 9 9 9 *>; m v i Note:' Affected fish were lethargic and,exhibited a loss of ^.^equilibrium. <; ' ->V-;''i!~<-&bj ,* vr i *4*' - '-nVae *A .t.\ -i '-'Vi, * fe*?**A'&&sc>'li ' i & w > i l{ ,r v:/Vj>**/5':7v*a:.V IS 8S&. : % # | *.Fsi ?V->V' M;' vfc ti &% 001228 t . ' '.Vv; -C ' V-.-' '* ' .Vi' ; '* tfc* .' i*Vr T S .'*-; $ 0 ru ,Exposure period LC50 24 hours >1,000 ag/Ii 48 hours >1,000 *g/L 72 bours >1,000 ng/L '96 bours >1,000 ng/I vV" ': 95 percent confidence U n i t `r. c-.-v - . y ;; j ..v . --f.i-'.'y :- -*; ` ' - 5 ` ; :LC50 calculation Method -- -- -- -- "V': :0 X 'sM v^ i ' - ' ; r .. ~,g r/Shp'K k* * *i> y ;.f> 001229 BEST. COPY AVAILABLE vii. RErancss OECD. 1984. Guidelines tor Testing of Chemicals. Section 2: Effects on Biotic Systens. Method 203, fish Acute Toxicity Test. Adopted April 4, : ^. I* BEST COPY AVAIL, Tbl* A.l. Conductivity, pH, tenpenture, and dissolved oxygen concentration neasured during toxicity test U jr, ,, Iv-' |jf.' 'c v \';frV> -1, Mp *, , concentration {g/U Rep. Conductivity (uaho/ce) 0 24 48 72 96 hr hr hr hr hr PH 0 24 48 72 96 hr hr hr hr hr 0 (control) 1 2 150 1 2 250 1 2 400 1 2 600 1 2 1,000 1 2 830 830 840 840 850 830 840 840 850 850 840 840 850 850 860 840 840 850 850 860 840 840 850 850 870 840 840 850 850 860 840 840 850 860 860 840 840 850 850 860 840 840 850 860 860 840 840 850 860 870 860 850 900 860 870 880 850 900 910 910 7.7 7.3 7.1 7.1 7.4 " 7.7 7.4 7.1 7.1 7.4 7.7 7.5 7.3 7.2 7.4 7.7 7.5 7.3 7.2 7.4 7.7 7.5 7.3 7.3 7.5 7.7 7.5 7.3 7.3 7.6 7.7 7.5 7.3 7.4 7.6 7.7 7.5 7.4 7.4 7.5 7.7 7.6 7.4 7.5 7.5 7.7 7.6 7.5 7.5 7.6 7.7 7.7 7.7 7.5 7.6 7.7 7.7 7.7 7.5 7.5 * - >:JC-rr&rr~*- . Ilifyii.- l / Tabi* A.l. Continuad. Nominal concentration (g/I<) Rep. Temperatura (C) 0 24 48 72 96 hr hr h r ,;hr hr Dissolved Oxygen (mg/hi 0 24 48 72 96 hr hr hr hr hr hi,.-', a IIfSNf,vf^tAiVC.s-,*.. 0 (control) 1 2 150 1 2 250 1 2 400 1 2 600 1 2 1,000 1 2 22.2 22.2 22.1 22.2 22.4 21.8 22.0 22.0 22.1 22.4 22.4 22.0 21.9 22.0 22.4 22.4 22.1 22.1 22.1 22.5 22.0 22.7 21.8 22.2 22.3 22.4 22.2 22.1 22.4 22.5 22.3 22.0 22.0 22.1 22.5 22.3 22.1 22.2 22.3 22.5 22.0 22.0 22.1 22.2 22.5 21.9 22.2 22.3 22.4 22.6 22.1 21.9 21.9 22.0 22.4 22.3 22.4 22.3 22.5 22.7 `^ ^ i 8.1 8.1 7.2 7.0 6.8 8.1 8.0 7.3 7.2 6.8 8.4 8.0 7.0 6.8 6.3 8.4 8.0 7.0 6.8 5.2 8.7 8.0 7.0 6.8 6.2 8.7 8.0 7.0 6.8 6.2 8.5 8.0 7.0 6.4 5.6 8.5 8.0 7.0 6.5 5.6 8.5 8.0 7.0 6.2 4.4 8.5 8.0 7.0 6.3 5.5 8.5 8.0 7.0 6.0 2.5 8.5 8.0 7.0 6.2 2.6 ,A'; ; ' -- ';--fe fcW, f j g %;.v *"S^VWsS^* ' &fVWMSiilu'M 1 ^V `- , .*,' ^Y4<`-A . r- J . p k y i V.. ..-. * $ iiS l &X'. l4P s.*t v S t f * J i tM ' i* '4 - i.'/* - W i v--' T-'S" ':->iy ' "??' ` V r ' S , >'> S&tf.: '?,. ;_t- % iiy ^ s * t $ ' I t ^ k ' *ynk$?tt: ^ _,->?" < *?!&*;.. .-'