Document pmBJnxRdeyK21YOb3Ev9a0odw
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AR 226 _ 3408
Validation of Determination of Total Fluorine in Blood by Wickbold "Torch with Direct Potentiometric Detection.
cPWorioricnnkdcnbi.iopllnedgF:uTsoourrcmihdp(WisIcc)oonimdbeaunnsstaeendadlaybtnyicyaadlbrssoaogmrpeblneiindoiyggeoesntnaiolmnmmeeothavonddsowmohvreerettheeTl0huoseimneeen.nisc
s(oFlIuStEio)ntioscthheinantaslyczoendcbnyidriaeisonpot(heenmieoamsenyedwisnogonM.oTrhids Ciomoesnmtsonsoeculeiyc
`proportional to the total amountoffluorine beingcombustedby WT.
Procedure:
Vs1.aplitVdaaltiidoantoifondoef
terminationofFluorine by WT/F-ISE in blood was
potentiometric determination of uid by FISE
perfo
rmyd
in
two
2. Validationofdeterminationoffluorine in blood by WT/F-ISE.
A. Validation of potentiometric determination of fluoride by FISE.
T1h1beCefatollwblreoawnittnoghnmceFutrIhvSoeEdOpEwotevessn.tsioeadlgofcfor2wd0apisembtepsorrteeefnedtrieaonmlceetyrsiwcohdeoerteenrOmnFindatttihonhFoefsomnisdd: asCoalllutlbiaoani.on2n0soptpanbnddraerafdes,rceonsncteahsionlicuntogino3cn0o,wra3a0s,tmo1endsosf10e0dnptiphmme=ecdwiuarrtlseyob,sesrerc.o ees
1.2 Performanceofthe F-ISE was cvaluated daily based on the deviatoiftohne calibration curve slope from the theoretical slope value (S). Considering that the F-
IaSlElowwaeds.operatedin the non-linear portion of its response,thetolerance of S + 5was
Where S- =Hat0
Wchoenrter.R universalgs constant, is empersorinKelvin, and Fis Frady's 1.3 aVpaplriodpartiiaotnesmampolesuofofnrdFi-tcIdSENmaeasurMeImKenQt0w.erhrieeaprerdibmy dsitsoscoklvwinng tshe was
sseorliuatliloyndsiiluntcedhiwintghwtahtoers roopmrosdaucmepalpeprwoproiaatemcionnceendtraatnidosnsoomfF inpwoamte:r. All F~
(HDPE) bottles.
1.4 shTaoemeeuvvnaalcuyeatraettahhoeeicfnaoctntteyurailfbiWuntaTiloWbnolTfoFao-dlIsStsEmepmelesaswrueorsuesml,enbict.maElnxtdceetpdhet,a`tsmhamepplbleeommarensgitppwulraotienontstoes nroatpnesrffroremdetd'.Tlhaatts,b1.o5lgeo0fswahmipclhet(hceeacoprpdredowpietthmanoalnytticoafl psrecsisimon)ewaas.d
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spe ot dd, The han of hon soe eed 50 GQ war (to simulate the final volumeafiertheWT burn). Theamount ofF" spike added ee es fn ef be WT) Testor it p1p50bg(,tthheescaomnetraisbutthieocnoonfcFe"ntsrpaitkieontooftthheefirneafleFr"ecnocnecesonlturtaitoinomnewaosuulrdedbebeefxoarcetleyac2h0 oE eptp innetge einus Aehmrenoehbm v msmShe1esemsgsbeeeamssheesnmngdeyoiivttsoeieoocnigenTdnemistasgertwsntdeeiyrmGeceedtof constant stirring rate. Aftereach 10-min measurement the electrode was dipped for 6
J TT. Results and Discussion
|
s rT n tise Srimiimatianghe
`Rmuletipplreiodendtiucaclwliayspberveiaplaluraietdetsdaaymtp1,le5s,a(nnd= 1100)0.ppmconcentrationlevels ; byanalysisof
`Operator tooperatorvariabilitywasevaluated byhavingtwooperperafortmtohe arbosve
`accuracy and reproducibility analyses independently.
Ae EteAEH Fyesi
eT keer|De concentration concentration ) concentration )
(ppm)
(ppm)
(ppm)
"suetesetbyetregone15 trdt mt (on 5
523
48
5.10
20
-
20
27
84
199
04
100
109
92
902
98
text)
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000175
Tables.
Reproducibility evaluationofdetoepremriantaortsi.onofF" by F-ISE at ppm by two
Actual F DeterminOepdeFratorrelative error| DelermineOdperatorreIlTative error
concentration concentration (opm) (opm)
(%) | concentration (opm)
(%)
55 5
355379
7i]7
459150
075
531
62
192
| 16
55
552675
15249
448950
2209
55
5s2i46
4al7
458046
3i1l
555
55025
a1a
44889
2S1a
517
39
485
29
D
wst.edevg.
03381
37T
[0209
%ev
347
1%
2032 :
dvioslpuomseatbrliecgglraasdsuawtaerdeugslaesdsfwoarrveoulsuemeotranvsofleurmse: transfers
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Table IIL.
Reprodauibily
evaluationof
determination
operators.
of
F
by
F-ISE
at
00
pa
by
two
Actual F con(cpepntmr)ation
100 110000 110000 110000 110000 100 ste.rdaevg.e %CV
Operator Determined F relative
error
|
Operator IT Determined relative
error
concentration )
(%) | concentration (opm)
)
1 97.1
52 29
110054
4332
110018
8133
112083"
232
110069 103
527 ] 33
91105
"8572
108
78
11003
29%5
110035
2si8
105
si
105
58
107 04
73 35
338646
29
44970
20
a*mveearsaugreset,m.ednvt.inafnludenScCedYbcyaexltelnsaivete(ixopoeosntureseo)fF-ISE to wate, result excluded from
srtelaantdiavredidtdecevaviniaabttieiosonneafernnodfmo%trChmVet'nasobmloiefnsa1rle,p1cl,oincacanetdnetm1reaattsihouanrtetvhmaeelaunect)csu)arnoafdctpyrhee(casFisAiiSnoEdnid(ceaatsteeirdnmbdiiyntaa%ttieodnbyof . tFhienmweatthAeosrdidcnoadnincobatetesidembepymrot1hv0eebdreeasopuppletrrsoaxstiuomrma-mtdaeerplieyzntdewedon-tif,noTladbwlehIe, tmhoeraecaccucruarcayteanvdolpurmeeciirsiicon of `TgcolhnaecsesaawctacrruaertaiicsoyncsaawnpedlropeyraeencdailsfyioorznesodoflautthiieonndtipecrcaehtpneaidrqbauteyitiohndeasnntodatnvidomalprurdmodeveevtriwaahntseifnoernhsaidngudhreirCnFgY-thevaanlauleyssiisn. `ftTioanbtallhesciIamcluilbaortaetdmioWnpcTutarsovoTerlaurbtaeilnoegneds.1r.TeThshuiilstsiandigdsidftuoiortnmaolt1h0s0eefppapacmndtshmaamottprhleeessFah*mcpaoltednomcbaeenni2pt0urxlaadttiiliiounntoethndhsoeso it acodnvceernstereaftfieocntsoanmtphless.tandard deviations and 96CV's one would expect fom higher (isneTeaPbrlo"ecT,ehdeIur,reeasun1ldt6s)I.sfhfwohewreneieonlbetTcaatibrnloeeddeIwiIshieanlacllowuwadteeedrotnowearesssitgunsiinefdiwcaaatsnetwrao(ustehlgii.enrdg.uTsrohilenut.rieosnulftosrsJhSowFn
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`memnietnaesreiuqnrugeidltihibenraeptaxiiotnsst(ai2mm0pelpwepiblslorlneuoftteirobenenpcsoeulnfairdicziaeendtatfocouvraetlrhyastahsmiopgllhuetpi!oostnte.natnSidiaanlrscd.e,Caoiltnidsaeutqsauuseanoltlhyliytohnhese2s0r1c0.
tdphipenbganrnoeafsleyersbeinyscwceaomsmepadasorunirenegwmieatlnlhttahwnehyi2oc0bhspegprebtvsmedeaaf2fs0eucrpteepdmb.enTouhtteslrifeeofrorTtehh,eistdhaseiytpuaranotdbiolcneoramrciecsluacrtarisenydgftotohreownae.y
SdsioaslmrupetlgiaeorndineodrT.asbIulnbesocIradnqeudren0otnemexipnseiarmmiipmzleeenttih.niTsafbeee,1.10BoptphboFf*tshoelsuetrieosnulwtassshsoeudldasbewash
iesxdtuenet1)0ftotirhsetnfheaecc2et0stshpaaprtby1tr0oe0fpneoprtebenscttehaamntedaaarssdumriehlmiaeghneetfsftmecectaoswnucaresnetodrbrasitegirhovtnesfdtae(n10da1r0md0)uppcprhbodssmutaaclnedlsaerrd. This
fTreolhraWteiTvoelraynalhloeywseinslettchtasrlotduheeipo2on0tempniptgibahlrtseufceorrmepnecaersecdogrhfrolestyhpeolnoednsigtnegrofc ttohtehestsvaynsdtaermdsfbmilean.ndkFfososmrpcicss.almple
svsotaliluuteionanon)td.iTancofclauuvreoaniccdyedothbfiytshtseihtmsueactafisofunc,rtehbmeeecnsattuoasnfedsalyrlsdstthesemhorbeulsluadlntkbsef(oamrnetdahsteusrs2ea0dmppilnpebsdreetfsedcreeepnnedcniedngon the
omonneddaeesrru,,rttehdtabheifseo2r02e,0c1ap0cp0h,2rs0et,faen5rd0ea,nrcade,nsdceo2er0e,psa3rpt0onpAdpib1n.1(g)w.thoWe5rhe0epnthptebh2es0stapntpdaridas wthhaneirrceehdmfeweriaaelsnucgrereetsdolidlingthistohlniys
afwfaeyc.Ttehdenefdfnecottotnh Ss0ypsptebmmebslasnkurrerfemreennictsem.inMiomriezaecdcubryatmeeWanTsdoaftlaicnaeanrbreepgrreosdsuicone;dths
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c(`oefRpficrieenetrosvqnaolfafulueulsotrehhcrairlegniehbsemrttraattnielhdoaanrnycddus.r0v.Te9h9s9if9siwiseoarmlemsouoblssteuirppvpleoedrd)taeydsbwyetrheehfiagchtetrhtahttahne0c.o9r9r9elation
lreevseullst.s TiSnhieTnacrbeelsteuhle,tseIff,freoactmnsdtahIneldsewoeebrssetersovbaatrtaeiiosnneusdm,dmiaasdrcdiuizstesideondiasnlbTtoaevbsetliecnog1uw.ladsInnpottehribfseotmrabmaleded,ea0tu.n51i5lpsptahmmepFl-e
cphspainbegewssaoslduuitssiecoduns(stueosdesaidambusolwvaeatwesethhresoeilxumttpeilnoteno)m.fenditleudti(osntafondcaradlsWmeTasubrleodoidnsdaemspcleensd,inagndovteer, 10 .
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Table IV.
Roped cratonofdtrninton oF by F151 pn 05g sample size
TO T AdalFp Determie ned reln ative error
concentration concentration
(opm)
(opm)
)
110
L3i
52
10 10
098 101
1076
1100
11.0010
09 02
|
10 10
093 092
+Ex0
1100
008874
1a63
Twenge st dev.
000m9
670
-- %CV
s96e4
8. Validation of determinationoffluorine in blood by WT. "tTehremsmoeftahcocdufroarcyde,treerpmrionduactiiboinloiftyf,luaonrdincearirnywovheorle blood by WT/F-ISE was evaluated in rAebcslcuoulotrdsapscraymoopdfluetcshesddpbeiytkeetrdhmweiimnteahttikhonondowwcaonsmeapvmaaorlueundatttsoeodtfbfhlaeutsoaerrdigoneent.etdhfel%uorreilnaetciovnetdeenvtisaitinotnhoebfltahnek. pRateeprtrfhoeodrsumcaeimdbeialfsiltwuyeo/lrplirneaecsciaosniconennwtaarsaoltefivoyiandleeusvnaettliee.cdalMsbluaylstpeirdpelopenaramenldaltsypasmlepseoleafnstaohlynesdseisafmofefesrsaeamnmppdlaleyeos.wperreepared Cdaabusmroiylnuogtvesefyrlsuetoveramilunbaeltaaimnooknudnwettasesrdmdeiotnneaertimboiynnce(obdmeifpmoamrreeidtnighasttheaelryaabofsfeoarlnutythesfaalnumaoplrlyiesnieasanoamlfoyuehniitog)shwdfieltuthoerrinmei:ned sacmonptlaienwiangsbsaumrpnleedsa(nadptprreos.i2d5thaensda1m0e0wpapmy)a.sFiof rittwheasse.derteearlmbilnoatoidosnasm,p0l,e0.000g af
`
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Aulolrtihneabtleododdifeto. FtlhueorWinTevsapliikdeadtiootnowbaslfrowomamsaoilnetdrhaetsfnoortmorfesatqeudeowus isaonlyusttipoenchioafl `awmemreowneiiugmhepderafnlduodriososcotlavnecdatinew(aabtbere0viaptreoddausceC8t)h.eAtpaprgreotperdiscotnecaemntoruanttisoonsfosfolfilduoCr8ine in ``awteeirg/hbtl/owoedi.ghAtlblatshie isnamorpdleerptroeepanrsautrieotnhne dbessttapnodsasridblCe8acpcruerpaacrya.tiEovnewraysfidnoalnebloonoda ``ssaammpplleespcornotcaeisnseedd9t0h%rooufgbhlWoTodwmaastr0i.x5a3n(dme1a0s%uroefsdpiwkiet.h aSnaalmyptliceaslipzreecoifsailoln)b,lood
Table V. Accuracy evaluationofWIF-cIoSncEendtertaetrimoinnaletvieolsno.f Fin whole rat blo"od at low
TO: ActualF concentration (ppm) 01o7 015052 418012 Apparatus: WTL
Determined F concentrafion (0p1p3m) 020 017007 a084
Temi(v2e)error )= 4297 "277
lo
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Acme andcaro ronsTfabWleIVLS. Edein hee
blood at high concentration levels.
TT
AdualF
concentration (ppm)
Determined F concentration
velaiive error 4)
System Blank F-- content
Tosa
(ppm)
8) am
o
-003
-
10.18
1048
29
sys.2b5l4a2nk 2
23.64
0
0769
101.36 sys. blank 3
96.04
52
| 0879
Amy by: Operior Apparatus: WT1
1
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Reprodacbilty
eva
l
u
a
t
io
n
of
WE
Table VIL.
IS detemiosfsFtaiwhooln
r
t
blooad
high concentration levels.
F (TAIoT cponmc)enr tration cDoa entceremnmetdration m0e mer----
a B2m34w6 m (2o4pm0) p 43
TT
246 wes
2160 ms
a9 ph ------
st. d%eCviVat.ion Amal by Operant
308867
37 \
|
Apparatus: WTI
2m56e0
2w5m0
2560
2610
2a1g 20
TT ostw, deevsiastion
x10n7
%ey
an
25
~~
RAupaplayrsaitusb:y:WOTp2eron 1
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radu
clotioof
Table vilL
WISE determin
ofi
whol
ood
Tow concentration levels.
ACAI concentration (ppm) Determined(Fpmco)ncentration Teme4)srr
4759%6
145875
2or3
449966 496
44971
3a3
478
356
Ta T st.wd%eeCviVaet.ion m 015098) a is
TT LLoont
093
a8 --
099
24
TT st.wdeeviastion
0I0w8
531
|
w %CV i 786 m --
|>
101 L1o0t1
095
55
009952
2618
TTT
101 wes
st. deviation
096 ow
----4
009
34
| |
T w0e52 m 05w42e93 er --
:
003522 052
004562
028
037
2
J
Tw T st.wd%eevCinaVtsion p 1020064 n
BEApparatus: WTI -- - --
2
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000183
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a)
=
"The results in Table V1 indicate tht theWTmethod for determination ofF in
ovbbalsloeuoerdsvedmdo:eeshonwoaetvseasurff,tfueewrr2efr5dr.oo4me2nsaoidtngbndeifli1icvane0tthcpaa1rptrtmy.hosevaedm3rpalise6scf.anAebes.laejirugedhnstgiegicndnicffreirecoaassmnetsiynesntvoaeulmugbehsl(ai0nsk
impact our results since the
containi"Tnhgleeascsctuhraanc2yo5fptphem
method is intended o
omefthfloudoriwnae.s evaluated
be used primarily for samples
across the concentration rans.
f
rom
e0cl.o1tm7il1va0ete1rd0o1w.ri3t6vhpatlphumesraesipnrciorndeduaicscieabtiweliditbthyydrdeeacsrauelsatshsioinnwgnTFaincbolTneacbeVlnatenraVdtIiVo1nn.,dTrheVeaIscIeh,dianAgtss2se7hx%poeu1ctt0e.db4,c5thpe m
l"deTivhfeeflr.reenptroodpuecriabtiolirtsyuosfitnhgte moetdhiofdfraetnatpWprsox.imTatheelyre2su5ltpspfmrowmastheevsaeleuxatpeedrtboystewuoare
summarized in Table VIL. It canbe seen that t these concentration vets both oparstors
`aaatccchciuoernvcaeecdnyta(rpcaptarilooixnvisemeoartfre5olrpysptomhfeasnad4.m3l:e+or3we.eprr7oa%rduacnsidbhio3l.wi4ntyi(n2%.TC5a%Vb)'i.soVTIfhTe3T.r8ae6pcraohnddudcaibti4.l1s1ie%tt)yiaosvhaweetlisloaanes
`ewirnaecssxruaenaspsaiilnnygdezieccadoarntbtceyehitnithtghrthemabstcoiaoteonmhnc.epneoArpplietttrhsiaoootsunongrsh(((tO2ip5s4epromapCt)oearurV1nsa)diot'nnniogctddrushirfenafos)ectaryCemon(cntddeaauapcyptsva.ehrAeaesrreorxspseefcoteppdrh,eototvheeesseith
acchoienvecacecenpttabrhleerarsetusluissoiunsniTnasgbtl,heeVcIuIrIraenndtapVpIariantduiscaattethihghteretshhaonu3lpd esnf be bie vo
concentrtaitsioinnst.erestingt0 notthat hisoperatorachieved worse reproducibanldy
(TalaactcbeurlretaiVcmyIeaInt.d2T5ht.hi60spmoppaemyrabtuesoirndghuaeWdtTtoth2h(eoTpfapabocrltetuVhnIiaIt)ythtoh4a.gnha9i6enpampcohmrieseavemexpdpsleetrsi4wn.ec9e6rpewpiatmnhalWuysrzi.endgaWt aT
r`oepcpeorrmoabdtuuiscntigiboiWnliTmteyoatpfhpotadhreaitnmuesgteihnoedor.nae.ToThfhtiihsseiwiaomlupsloodrstsuaupngptgoerfstatcetdtohrbasytittnfhhloufpeaencrtcaittnhogarttthteehceahocntcihuqeruraecoywpeharinaldteor
was not ble to achieve accurate and reproducible results at concentrations of ppm and
locHiownnekcerlduuss0Oiipoanengrscapbateoncotirbhfei|WcwmToaa'psdesrehabottywhoeratvheuaesrtti,lnioagmwbealctesohptneeoccdeiganfettinacreaarWtbaitoHoevneaqpulwpaeeavnretrlaiesttuagtsteh.ineveserelayvtaevldai.ldiTadthdieoranetfdoaurtsesi,nsgahoWuTld?baeta
aptcapthmeireFtvieimdDnea.ebtulTaoihoivednas.lTeeTadnbiatlstepariVaserIcieiInsdsiaiuoncmnadmtsaeIndrXdibizaynedcsdiiucmiairtleTaaatrbhvalatetlsOhvpe1eescoroafntXsoctreIaiUnrdaWair?odndaeovnideaOtipooefnrsap,tporro1t/mWaTc1ly 5
h(pirsecciosnicoen)n,traantdio%n rleevlealt.ive rors (accuracy)ofthei respective replicate mesaescuurreements at.
2D
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000184
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er Reproducilycalusion ofWIESEdtrminofaFitwohons ot bon a5 ppm"
ST (ppm)
(%)
------ 8 -- asa
* samples separated by lines were replicate analyosftehse same: sample within a particular day
d*ivfaflerueenntottaursgeetdfFcoornacveenrtargaet,iosnt.andarddeviation,and %CV calculation because of 7
2
:
000185
|
Ev
en
of
Table X.
backgfuroronulvnlsd
nbs
ra
t
bloodbyW
IFI"
a
Sm(pipmt)e
------ (ppm)
`blank blood
0.10
blank blood
037
e `bilnnankkkbbloloooododd" e 0o0.n%10
beg --------
`blank blood
029
||
TT
Sank ood blank blood
lank blood
0%024
026
hblYeasnekoebwlooond
aJ0.1y)5 ----
D
TT: pe
Apparatus: WT2
|
|
* pis epi les we pict ly oftesmesupe iin
particular day
|
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000156
|
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RepradciiclsofWIT1T5abldeXLi. noaf whl at bond
ppm*
"cACoTFvpmc) eatrDeacotnecretnmtirnaietioFnon
-- 104
om)
106
T 02 em 120 ----
111.000000
L11i11s96
|
or a |
1L1.o00o0
111.000956
T 104 e i12m4 ----
|
T 102 m T12e9 p-- --
Jan
wim
|
ToT
122
|
i|
1LLo0oi1t
1112268
101
12
|
mi
hLLi1az2
111122336
T st. deLvizationes i0L0wi6o9 --
`Aapptaraitubs%y;:CWOTYp2ie]
59
*samplesseparatedbylineswerereplicateanalysesof the samesamplewithin a.
particular day
000187
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}e D
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|
Tem.
Reproducibility evaluationof WI/F-ISE determination oFfin wholeratblood at
0.5 ppm*
ATS bi e r De T
----=------G(o-- ppmem) --
--_-- 2055
on079
-- --F05H_ _ 2 -- --406-- -- 4 '
-- -- eoee
=Fi
Tpeili Gp
----
*samplesseparatedbylineswerereplicateanalyses particular day
ofthesame
samplewithing
|
drenpettenCsthautpteoii:chTaeomy hooienaafspeae daat rl eascone hX TteragolntXdr1dlaaedogtX.eel [saohtvobtwsietbsonhfekeear m etsBe e odnd.t te hesSterreidtoreoffe `erneboanconkdignoroTuenoawdesDeFtlmelevsedlAf2ot3er5e tpheestbeesamTpe hlees,blaos nkabloepordsMravmspllrleonsedwsSere=omawontoMah1lalyrz.ke1mebdlt uhoesnridngtelhcreoesiamasramre a Ta onutmst ri aa o lecnbe deel eXdeL concentrationisabove LOD ofthemetthhevo aludec, anbe deemedtobe dueto
background F are shown in Table XIII.
5
000188
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8
Tol
bod
sme
12nd
Table X11.
0.5py ns
hor
abe Xl comets
for background F.
concentrAavetraigeaocinual mAevaesruargeed
pm) concentration
lark (v= 13) (p0p2m4)
103 (0-27) 052(=9)
118 070
`ApparatuRsus:by:WOTpe2rator?
Sdeavnidatairodn (ppm) 009 0069 0061
coFrroectoedlfor background
(opm)
[x 046
Vierrrore. n
+9 13 )
bpoascskigbrloe"uTonanbdllyeFXbienIcbalusashneoktwbhsleotaohvdae,ratafhgeteerbetaschukscgorsrorhueoncwtdiaFocnocceofpnttchaebneltaerpapatlciicocunarabiclnyeb.lraeTsnhukilstrsactoobrmleotcohiedownawsaast
tdsheutebetLrrOamcDitneode.dfb1tlhasenhmokebutlldhobooeddwcmoientnchteitnhotenrOeapdteihrooanwtceoavrenrtW,heTrhe?aftotr(hesebeseetTadatebeeldemvXeaIldutVeofb(osercedLuTOcaDbtldoeFaXa)an1dTcohalenybaen
ibenslttohioimdsadtbeeetcebareumcsiaenuoasfteitothnhe.eFvIatclosumreepwaolasisisttuiincotdnoeorfettxhhpeeeiLcrtOsdiQoetm.(eTTabhbaelcekFgX-IrIVSo)Eu.nmdeHletenhvcoeedl,odftehsecirgirnebbaetdlviaanrnrihaaikbtsility
recfpaoconiraltiitnwyeawdsatu0.esretpdhpatmoeivagleIunnactareatdlhynefc,eodnc1tehensttthreaanatditaosnriodnfrtohodeneHinaztsakdbleieltli1Lsanbocroaotnortryag.ipnTsahtehFiennwahneieirmmcanlge of
tb8ha-ec15kFpgcrpoomnut(neddnatFtiacnostnhcuiesnptdriacpttbi(loyanlPi.muoWrcseitndh1aa0Md0i%nlolv,aidrniidaeibtcisalutiiptoypn)litneohtra.atlBlheetchbaeluobsoledooofudtsewhidelflogcreoadnttavaniangretinhaeertsaiatoemsfne
lbeyvOelpse.rTahteobrlIo,oadnudseOpdebryatOoprerLaatto5r same baoftanicmalhs.
p/pWmTe2vefolrcbolnatnaki,ne0.d5
aannydde1tpecptmalbelveeblascckagmroeufnrdoFm
the
dDeteermitnateionoorfftlomtailiFofmidnnebtlieoacottdiobtny(WiLIO/DFo)-aInSndE.limitof quantitation (LOQ) of
dmiesatsiubruetAmosensn,utmtshioenfgLcoOtnhDcateanthtnrdarLteisOounQlltescvapenlrsobdeculecosestdeimbtayotteWhdTe/fLFr-OoImQtShE(nbsaetha0anvdxeacracdcecdeodvrid1ai0tnxigoLtnoOo fGno)erpm0iatl.ate wbalsooedswtiaLmsiaemtsiettodabfalsiqsu3ahxneidhteaaktaiivonengra(igLnetOosaQtc)acnoodfuanrWdtI/tdheFev-aiIactSciEuornmaaectyhlnooedwefFdoercdoannfcoaerlnyttshrieasmtoaifojtnootrvaleyrfoleofcrine in
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u6xptchoemianvgertaogxeicsotlaongdiacradl dstudeiesvfaortiwlhoiwacFhctthoinsciemnettorhaotdnioins ilnetveelnsd.edL.OLDOaQndwaLsOeQstdiamtaatefdoras totalF determination in blood by WT/F-ISE by both operators are shown in Table XIV
TableXIV. LOD and LOQofdeterminationoftotal F in blood by WT/F-ISE.
TT "Operator UWIZ
"Operator TOWTT
Average a(ctouaplmc)oncentration AverFadgeetSerumnindaatrioSn (eppmv) ST
052B (n=9)akG- 0006%1D)
150032@--257))
00009639
TaOvDerCaxgae votgde.v)
0081 023
LOQ(6xave st.dev) 052-3)
0.49 7055
419061((aa==58))
0000787s
averay OD (3x avg. st. dev)
0m 022
LOQ (6x avg. st. dev)
oa
toatnadl LiOnDQawtwhiaotlshehtbohlweoinordiraesTipsa0ebc.lt2ei4vXpeIWmVT'wisni.dtiTchhabteoertetfhoaorpebe,orLtathOoorDp/eWorTfattchooermsbWaiTcn/haFite-ivIoeSndEse.dqeuAtinevyramlrieennstautlLtisoOnDof dtTehotawenecortretedhq"a.unaLtlhOi1sQ0ov0af.l2ut4ehepwpimelmtbwhieoldlrebipseor0r.5teepdopriptnemtdhaeansfdu*at<unr0ye.d5sa"ttuiadniodeiwsceaatsri"ntNghDatnh"a,0t.sF5tpapnrpdemisnebgnucfteohrwi"agnshoesr daectceucrtaecdy.but ts concentration was 100 ow to quantitate it ith the required degree of Aa,scetc."Td1uh.a2et)arpabiiesalrrttytfocfaeolaruftmooomafhante-eIscduSFecE-oIessSsEmhoefaWbosTeu/erFuv-eaImllSeuEnattdmeoedfdthaFolidyn.bWAasTsemsedanmbtypiltoehnseetddeocvpioartoeidounc(eaorft Yciitasmliritebsropafotinitossnep,ceturhrfevoserlsmolaponepcveeafalrsuoesmapegtchrieefietehmedeobrneyttdmiocaacnlsunsfloaotcptgeuuvraaerlauanent.edSewitinhtceheitsnchctuhFer-aIneSoyEnofifstnohcpeaerrepaaatlrettdyia,onAtoshfe oaSwrEeasmsuelbstsa,usmreedremoneanatquwseaosuuosufsraeNadFe.QT.uaThmlheieQteyCtCaosnrnagrmetotplelde("wQcaCos)nscpearnemtppralarteeiwoainnsidneiptenhendQteCnrtslwoymwhpdbelynueewvchaeisnredtp1hderpyp7. "Thissamplewastreatedthesame wayassamplesfromWT (samplesize .Sgweed,
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Ntmhaartorcuixga.hnAWdT`F,Q*sCtpsiskycaeslatcedumdlaebtldiaonnnkcd'awnfianlosletdpbreepb1aa5rs0eged)di.onSsnitneWeaTd t(shsyeasmtQpelCmessaibmlpalnek0.dw0ua0e0st0no5outtssepddri,ofcNfesasesndesdand `wFi*QtCshpaiinnkael5yas%didsebadasaienndddiofcinaltl"eedQd Ctboys1yt5hs0eter)mesabunlldtasnukss"hesodhweonxuclildnusTmiaavbtelclehyItfVho.erIQOfCChictsaalrccgrueilttaeetrdiiocononnicsAennrottorfasutaliolfneads, `aQmCceoartsrouerctehtmeivepenrtaoccitseidaouunrtesomhiaotmuelpdda.sbseHottwhaeekvesnearmt,oplehenisgtuhhrerecotuchgohnpfauictdceuonrncalecyyiosnlftihgFht-ldIyaStbEaedccaaaun,sbeIeanlorlbottdahuiecnFtei-do1an5n5di canalsobe usedas agrea troubleshootingaidwhentheresultsare not3sexpected. Conclusions
`EloemcbtrioadVtealohinadsawtbiietohennodipfrederecfttoerprmometiden.natitItoimohenatorfibtceoeftnlaulforofiluduneodrditenhteaetcitn-ibIolSnoEwoiddtehbtyeFrlWmuiiocrnkiabdtoeilolndoonTfoYfreculhcstiineve in
spsoalmueptlieocsn psciraouncsbsedsesiafecodrhottihherevnoeeuldgehcfWthrToedFie-srIionSpseEe.riaItnnodoritintxdipeopones,ncdcdeanlttiob.wraBatetiseotrnapsntodasnbdsauertdasecscdhuoru1at0cpybae.npdF-
`cwmoheneacsneuenrvtereadrtfpirooosnmssihabislgewhfeeoslrtvatnooldloutwmheesettlraecncostnfrceoresdn.etrpTaehtreifoocnrl,meacantndrcovedoeclhusehmoceuktlradiccndgoltsbatosersawegaxerpseososnhedodiuotlodnhbsieguh sF-ed
IoiSuttsEloimpneeeradasituinroentmsienmntahtneuafliQancCetausrapemsopmtlaienonwuaaoslfstmhheeoauselludercnetodrtoadbneedfretoshlpelovnoaswleei.ddibTteoycoeafnusistuesrtrehesteuhyleatsrcceoicmnutpreanadreoetdffFdoo.r
tthvheeatlthaeuroegreedttceicdaacclohnsclieomnpterivatatwliuaoen.vSuaslhuoerbwataseseradseoslneesctstheerdodddaeeilsvyt.iorTaahgtoeeifeslhtoehonceutlrcdobdaeedpleorniftoibrcnmuarr2nvcaeepstwlroayipseoronm
trheefemreetWnhceoTdsocdloutcisoonn.otmsufmfbeertfhooumdwsas isgneivftaiclaunati tceadfmyooorvbelrn.ooRdesparmodpulceisbailnidtyitawnadsafcocuunradctyhaatt
hrtiehgsahutlchtosenwrceeesrnuetlrtgsaetgnireoeranatllllyvyeedlqesupiwevaansldeenovtna.olpHueoarwtaeetdvoewrriW,thTatdilafopfpwraecrnoatntcuoespnectroramatbtoiirnosanitliteovhne,ldsi,wfhfiierwcehanstsuWogbgiseessrtvseTdhe
ttwhheeireiranepfaplsluciotencdacetaoorftaehnfyeulmoepcaeorsnaustiroderemrteaentctihsonnisrqehuqoueuiorlindngtbhaeedgjaiucvcseutnrmaiecfnytthsaenidndattpahreepcoripesseieornnttoeofd teihncehtmnhiesqtruhenop.a,rTthFiosr
hidiinfsfcveaellnit(dauaptipoasnrnaroteuptsolrtithmiaisntvetadhlei1yd)ouaneydnfoartgloeotnfyaedlriasfftlfeiueorterhsnietnvemadaletitrdeiarctiemosinnaandtaitotnhiinnotpbhleiororsedtpoosrsnt.dssTnhgpateis,
rmeoasfaptneycitdiasvtueacWhoIaff'supstlubaryswmshati,ucrhedtdsbheilytoigosedancselsralutm)eeadndttdhhaeftsoetrvhatelhieodppaaertrtiaiotcnourdlsaatrpaoe.prTefnroaratmdodrihsteviaoinnn,afgloytshvealidity
Wahcetnlymhoreesadamteabmeacnomiecsasvtahielyabiledfowrhdeifnftehreeyntgmeantcreitceeds `combinations, attachments o this report will be writen.
dat
and
presentedint epot
diffrentoperatorWT
"
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