Document pm97rkn0QMb94DvMb7yVQONq7

ARA.G _ 0 0 5 ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, may also be referred to as PFOS, FC-95, or as a component of FC-3072. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-3072. Current information indicates it is a mixture of 1.67% test substance, 33.33% diethylene glycol butyl ether, 29.23% water, 20.83% urea, 5% sodium octyl sulfate, 3.33% sulfonated alkyl ether sulfate salts, 4.60% acrylic foamer, 1.67% triethanolamine, and 0.33% tolyl triazole. The following summary applies to the test sample as a mixture of the test substance in water solution with incompletely characterized concentrations of impurities. Data may not accurately relate toxicity of the test sample with that of the fluorochemical component. METHOD: Method: OECD Guideline 203 Type: Acute static GLP: No Year completed: 1995 Species: Pimephales promelas Supplier: Environmental Consulting & Testing, Superior, Wl. Analytical monitoring: Temperature, pH, conductivity, and dissolved oxygen. Exposure period: 96-hours Statistical methods: LC50 values were calculated using the Trimmed Spearman-Karber Method and the U.S. EPA Computer Program for the Probit Method, Version 1.4. Test fish age: Juveniles Length and weight: Average length = 25.6 mm Average weight = 0.139 g (wet) Loading: 0.77 g/L Pretreatment: None. Test conditions: Dilution water: Dechlorinated City of Duluth, MN tap water, post carbon filter. Dilution water chemistry: Hardness: 47 mg/L as CaC03 Alkalinity: 45 mg/L as CaC03 pH: 7.96 Lighting: Cool-white fluorescent lights. A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. 002921 Stock and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis. Concentrations dosing rate: Once. Stability of the test chemical solutions: Not noted. Exposure vessels: 2 L glass beakers containing 1.8 L test solution. Number of replicates: 2 Number of fish per replicate: 10 Number of concentrations: one plus a blank control Water chemistry during the study: pH range (0-96 hours): 7.61 - 7.96 (control exposure) 7.25 - 7.81 (1000 mg/L exposure) Temperature range (0-96 hours): 20.0 - 20.4 C Conductivity range (0-96 hours): 1 1 6 -1 2 3 pmhos/cm (control exposure) 1 2 5 -1 7 5 pmhos/cm (1000 mg/L exposure) Dissolved oxygen range (0-96 hours): 6.2 - 8.8 mg/L (control exposure) 4.7 - 8.8 mg/L (1000 mg/L exposure) RESULTS Nominal concentrations: Blank control and 1000 mg/L Element values: 96-hour LC50 = >1000 mg/L 96-hour NOEC = 1000 mg/L Element values based on nominal concentrations Remarks: Testing was conducted on the mixture of the test substance as described in the test substance remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS The test sample 96-hour LC50 for fathead minnow was determined to be >1000 mg/L, the highest concentration tested. No mortality was observed. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY ________________________________ Reliability: Klimisch ranking 2. Testing meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. 002922 REFERENCES Test was conducted by AScI Corporation, AScl-Duluth Environmental Testing Division, of Duluth, MN at the request of the 3M Company, Lab Request number M3597,1995. OTHER Last changed: 6/25/00 002923 BEST COPY AVAILABLE ASct Corporab<m/Acl-DuluCb E a v im w a ia ] T n ia f D w AS cI Study ID#5010-0061 STUDY TITLE ACUTE TOXICITY OF FC-3072 TO FATHEAD MINNOW (PIHEPHALES PJROMELAS) DATA STANDARD OECD GUIDELINE 203 STUDY COMPLETED March 20, 1995 TESTING FACILITY AScI Corporation AScI-Duluth Environmental Testing Division 112 East Second Street Duluth, MN 55805 Tel. No. (218) 722-4040 b e s t c o p y a v a il a b l e AScl Cafporvtkm/AScl-DuMMh I1vir-- r i l l Testing Dwicn AScl S h Jy IO SOIO-OOM 1.0 GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Request# Testing Facility Principal Investigator Project Director Testing Facility Director Definitive Test Dates Data Validation and Report Review Acute Toxicity of FC-3072 to 1 Fathead Minnow ( Pimephales promelas) B OECD Guideline 203. 1 3M Environmental Laboratory, Building i 2-3E-09, 935 Bush Avenue, St. Paul, I MN 55106. - I Susan A. Beach, 3M Environmental Laboratory, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55106; Tel No. (612) 778-7452. M3 597. AScl Corporation/AScI-Duluth Environmental Testing Division, 112 East Second Street, Duluth, MN 55805; Tel. No. (218) 722-4040. Joseph Dierkes Joe Amato Donald Mount March 13-17, 1995. Alan Mozol fL:y<y^~Report Signature: Retention of Raw Data and Final Report Location of Raw Data and Final Report Two years from the date study is completed. AScl Corporation/AScir-buluth * ' v Environmental Testing Division, 112 East Second Street; ;,Duluth, MN 55805; Tel. No. (218):722-4040. | j f *'" .s ' &'v'' 'V :%I1- V * Spauor: 3M Caapaay SpoMor Study 0>* M3597 Ti, v;.- * i <-,:;a W k im$k w$' i ' >. &A < -> .u*.' v -4 .* . . r '-'-l&-t- ** OfjL.g 002925 V BEST COPY AVAILABLE AScI Corpanbcm'AScl-OuJMk E a iin M lilT a M i D iv i AScI ID # SOI0-0061 2.0 OBJECTIVE(S) To determine the 96-h LC50 and 96-h NOEC (no observed effect concentration) of the test substance, FC-3072, for fathead minnow (Pimephales promelas) under static test conditions. This study was conducted according to the AScI study plan Test Substance Test Organisms 3.0 TEST METHODS Properties: FC-3072, amber colored liquid, specific gravity = 1.1 (water = 1), stable, and soluble in water. Test concentrations: 0.0 (dilution water control) and 1,000 mg/L. Test solutions prepared by direct addition to each replicate test chamber. 1 1 1 8 Source: Healthy, juvenile fathead minnows (Pimephales promelas) obtained from a commercial supplier. Holding/acclimation: Held and acclimated for 12 days in the test dilution water. 0% mortality occurred during holding/acclimation period. At test termination, they were 2.2-3.0 cm in length and 0.108-0.193 g in weight with a mean of 0.139 g. Use in test: Did not feed 24 h before test , initiation. Used 10 organisms/replicate and 2 replicates/test concentration. .v . 002926 BEST COPY AVAILABLE AScI C a p o n o a i/A S cl-M u ifc Envirn-- f U l T a iia f Dtvtaica AScl Study ID # S0I0-006* Dilution Water Source: Dechlorinated City of Duluth, MN, tap water. The most recent chemical analysis is in Raw Data Package. Exposure Chambers Incubation Characteristics: At test time, the water had a hardness of 47 mg/L (as CaCO,) and a pH of 7.96. Aerated for more than 24 h prior to using in the test. Type: Pyrex 2-L glass beakers. Use in test: 1.8 L of dilution water or test solution/chamber. During the test, kept covered, except when experimental observations were made. Solutions were not aerated during the exposures. Duration: 96 h. I ] 1 | | | Daily photoperiod: 16 h light and 8 h dark, I maintained using cool white fluorescent lamps. | Observations Temperature: 20.0C. | Biological: Daily- Test organisms with abnormal behavior and signs of stress, and dead test organisms. Endpoint Calculations rj ' l : - ' . " ` Water chemistry: (1) At test initiation- total hardness and alkalinity, (2) dailytemperature, DO, and pH, and specific conductivity. All determinations were made according to APHA methods (1989). 24-, 48-, 72-, and 96-h median lethal concentrations (LC50's), and 96-h no observable effect concentration (NOEC) m tf: BEST COPY AVAILABLE A id CocporaUW ASc1-D uiudl Pmrin-- r n l T c a tit V rrim m A M SMdy tD # 50100161 3.0 RESULTS Test Substance Toxicity 24-h LC50: >1000 mg/L. (Pertinent data are in Table 1) 48-h LCS0: >1000 mg/L. 72-h LCS0: >1000 mg/L. 96-h LCSOr >1000 mg/L. Ranges of Water Chemistry Parameters 96-h NOEC: 1000 mg/L. Total hardness (as CaCOs mg/L): 47. Alkalinity (as CaCOj mg/L): 45. Temperature (C) : 20.0-20.4. DO (mg/L): 4.7-8.8. pH: 7.25-7.96. Specific conductivity (nmhos/cm): 116-175. 4.0 TEST QA/QC QA/QC Criteria Mortality in control exposure must not be more than 10%. The lowest DO concentration at test termination must be at least 60% of the air saturation 1 value at test temperature. Test duration must be 96 h. Data | 0% mortality. 'i-j V S/.V'--.- ' The lowest DO .concentrationV was '52% of the air saturation value at 20.0oC.^ Although the DO concentration was below; 60%$J. saturation u n ithe'5l0,00.jimgyL# test concentration thetest- 4 appears valid due to the"''X-fc" lack of effect. --*>, '> The test duration Viras f9 6 :i 002928 BEST COPY AVAILABLE A S cl C erpoM tooe/AS el-IX iM b E a vtro M ca ta l Testing D m iia a A S cl Stetfy ID # JO10 0 0 6 * Reference Substance Toxicity Test (this test is optional). Final report for the most recent test with sodium chloride is in Raw Data Package. BEST COPY AVAILABLE AScI CnpoMa/AScl-Dutuih fill irn-- ralil Teujpc Diviaiaa AScI Study ID# 5010-COM 5.0 REFERENCES American Public Health Association (APHA). 1989. Standard (Methods for the Examination of Water and Wastewater. APHA, Washington, D.C. Organization for Economic Cooperation and Development (OECD). 1981/1984. OECD Guidelines for Testing of Chemicals. OECD Publication Information Center, Washington, D.C. Hamilton, M.A., R.C. Russo, and R.V. Thurston, 1977. Trimmed Spearman-Karber Method for Estimating Median Lethal Concentrations in Toxicity Bioassays. Environ. Sci. Technol. 11(7): 714-719; Correction 12 (4): 417 (1978). US EPA EMSL Cincinnati, Ohio. Computer Program for the Probit Method, LC50 Estimate and 95% Confidence Interval. Version 1.4. BEST COPY AVAILABLE ASc! Corpomno/AScI-Dulutfl Bavirn--if te l Tcitim Dmcioa ASc! Study ID# 501D006S Tabi 1. Acute toxicity of FC-3072 to fathead minnow (J>. proaolta) Test substance nominal concn (mg/L) 0.0 (control) 1000 Cumulative number of dead organisms and % mortality* 24 h 0 (0) 0 (0) 48 h 0 (0) 0 (0) 72 h 0 (0) 0 (0) 96 h 0 (0) 0 (0) `Percentages are in parenthesis. LC50 and NOEC 24-h LC50 48-h LC50 72-h LC50 96-h LC50 96-h NOEC Effect and no-effect concentrations with corresponding 95% confidence intervals Data (mg/L) >1000 >1000 >1000 >1000 1000 95% Confidence interval Not calculable Not calculable Not calculable Not calculable Not applicable |